CN106006655B - The method of Ostracode microfossil is extracted from black shale - Google Patents

The method of Ostracode microfossil is extracted from black shale Download PDF

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CN106006655B
CN106006655B CN201610320977.0A CN201610320977A CN106006655B CN 106006655 B CN106006655 B CN 106006655B CN 201610320977 A CN201610320977 A CN 201610320977A CN 106006655 B CN106006655 B CN 106006655B
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sample
deposit
black shale
volume
abundance
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CN106006655A (en
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陈亮
唐振平
刘江
谢焱石
王正庆
刘珊
黄伟
胡杨
冯志刚
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University of South China
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University of South China
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    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
    • C01B33/00—Silicon; Compounds thereof
    • C01B33/20—Silicates
    • C01B33/36—Silicates having base-exchange properties but not having molecular sieve properties
    • C01B33/38—Layered base-exchange silicates, e.g. clays, micas or alkali metal silicates of kenyaite or magadiite type
    • C01B33/40—Clays

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The method that Ostracode microfossil is extracted from black shale, specific steps are as follows: black shale is ground into particle, particle volume is 0.2~0.5 cm3The black shale for being ground into particle is put into container, first pre-processed with hydrogen peroxide and sodium carbonate mixture, after filtering plus water heats, after object to be deposited scatters, be sieved with sieve, then with tap water repeated flushing sieve in deposit, then deposit sample after acquisition wet screening dried, dry screen, choose sample, finally identified and counted.Statistics is converted by volume number, i.e. 1 volume, 10 individuals, entire 4 volume of sample, 40/sample of abundance, if 1/16 volume contains 10 individuals, then entire sample abundance is 160/sample, and the total abundance of deposit sample is equal to fine granularity deposit sample abundance and adds coarseness deposit sample abundance.

Description

The method of Ostracode microfossil is extracted from black shale
Technical field
The present invention relates to a kind of method for extracting Ostracode microfossil, especially one kind, and Jie's shape is extracted from black shale The method of class microfossil.
Background technique
Black shale is a kind of sedimentary rock rich in organic matter, and distribution is wide, is the good of reconstruction paleoclimate and paleoenvironment Material.The rocks are generally formed in the depositional environment of anoxic, and therefore, most of scholar thinks wherein seldom containing zoolite. Currently, more and more researchers have found that there is also many layer positions for being rich in zoolite in black shale, such as microbody animal Fossil --- Ostracode etc..Ostracods are the good index for rebuilding paleoclimate and paleoenvironment, thus the section from black shale It learns, effectively extract the quasi-fossil and seem particularly significant.Though forefathers have done some explorations in this regard, it is stranded, is started to walk by understanding It is later, it makes slow progress, fails to propose so far to extract Ostracods method in a set of practicable black shale.
Summary of the invention
One kind is provided and extracts Ostracode from black shale the purpose of the present invention is overcoming the above-mentioned insufficient of the prior art The method of microfossil.
The technical scheme is that the method that Ostracode microfossil is extracted from black shale, including sampling, pre- place Reason, drying, dry screen, chooses sample and identification and statistics at wet screening, specific steps are as follows:
A, it samples, black shale is ground into particle, particle volume is 0.2~0.5 cm3。
B, it pre-processes, black shale particle is placed in container, addition concentration is 8~10% hydrogen peroxide solutions, black shale The volume ratio of particle and hydrogen peroxide is 1:10~25, and wherein black shale particle is no less than 50 cm3, in order not to destroy fossil, also 0.05~0.1 mol/L sodium carbonate liquor be must be added to as buffer solution, so that soak pH value is maintained at 8~9, solution Temperature maintains 30~40 DEG C, and the reaction time is 24~48h, removes organic matter, so that black shale particle is tentatively scattered, then mistake Filter, obtains pretreated black shale sample.
C, pretreated black shale sample is placed in container by wet screening, adds water, and the volume ratio of sample and water is 1:10 ~25, then ebuillition of heated, after object to be deposited scatters, is sieved with 200 mesh screens, then is deposited in tap water repeated flushing sieve Then deposit in sieve is transferred in complementary tank by object, to be dried.
D, it dries, by the sample after wet screening in baking oven in 40~60 DEG C, toasts 40~50 h.
E, dry screen, the deposit sample after drying first crosses 40 mesh scalpings and obtains coarseness deposit sample, thin after 120 mesh Sieve obtains fine granularity deposit sample.
F, sample is chosen, Ostracods are concentrated mainly in fine granularity deposit sample, but coarseness deposit sample sometimes On also have a large amount of distributions, therefore two granularities are intended to choose sample, when choosing sample, by fine granularity deposit sample and coarseness deposit sample This piles the strip of an identical thickness respectively, is divided into N parts by length, wherein N >=2, choose sample under Stereo microscope into It goes, amplification factor is 20~80 times under mirror.
G, identification and statistics, when identifying amplification factor be 60 times either 120 times or 180 times, statistics by volume number into Row conversion, i.e. 1 volume, 10 individuals, entire 4 volume of sample, 40/sample of abundance, such as 1/16 volume contain 10 individuals, then Entire sample abundance is 160/sample, and it is heavy plus coarseness that the total abundance of deposit sample is equal to fine granularity deposit sample abundance Product object sample abundance.
Compared with the prior art, the invention has the following features:
1, method flow provided by the invention is simple, easy to implement.
2, Ostracode microfossil is extracted using method provided by the invention, the shell-breaking rate of fossil is low, and shell-breaking rate is no more than 15%。
3, more more scientific than the conversion of conventionally employed mass number using sample abundance is calculated by volume number translation method.
Below in conjunction with specific embodiment, the invention will be further described.
Specific embodiment
Embodiment one, the method that Ostracode microfossil is extracted from black shale, including sampling, pretreatment, wet screening, baking Dry, dry screen chooses sample and identification and statistics, specific steps are as follows:
A, it samples, black shale is ground into particle, particle volume is 0.2 cm3。
B, it pre-processes, black shale particle is placed in container, addition concentration is 8% hydrogen peroxide solution, black shale particle Volume ratio with hydrogen peroxide is 1:10, and wherein black shale particle is 50 cm3, in order not to destroy fossil, it is necessary to be added 0.05 Mol/L sodium carbonate liquor is as buffer solution, so that soak pH value is maintained at 8, solution temperature maintains 30 DEG C, when reaction Between be 48h, remove organic matter, so that black shale particle is tentatively scattered, then filter, obtain pretreated black shale sample This.
C, pretreated black shale sample is placed in container by wet screening, adds water, and the volume ratio of sample and water is 1: 10, then ebuillition of heated after object to be deposited scatters, is sieved with 200 mesh screens, then with tap water repeated flushing sieve in deposit, Then deposit in sieve is transferred in complementary tank, it is to be dried.
D, it dries, by the sample after wet screening in baking oven in 40 DEG C, toasts 50 h.
E, dry screen, the deposit sample after drying first crosses 40 mesh scalpings and obtains coarseness deposit sample, thin after 120 mesh Sieve obtains fine granularity deposit sample.
F, sample is chosen, Ostracods are concentrated mainly in fine granularity deposit sample, but coarseness deposit sample sometimes On also have a large amount of distributions, therefore two granularities are intended to choose sample, when choosing sample, by fine granularity deposit sample and coarseness deposit sample This piles the strip of an identical thickness respectively, is divided into 4 parts by length, chooses sample and carries out under Stereo microscope, mirror decentralization Big multiple is 20 times.
G, identification and statistics, amplification factor is 60 times when identifying, statistics is converted by volume number, i.e. 1 volume 10 Body, entire 4 volume of sample, 40/sample of abundance, such as 1/16 volume contain 10 individuals, then entire sample abundance be 160/ Sample, the total abundance of deposit sample are equal to fine granularity deposit sample abundance and add coarseness deposit sample abundance.
Embodiment two, the method that Ostracode microfossil is extracted from black shale, including sampling, pretreatment, wet screening, baking Dry, dry screen chooses sample and identification and statistics, specific steps are as follows:
A, it samples, black shale is ground into particle, particle volume is 0.35 cm3。
B, it pre-processes, black shale particle is placed in container, addition concentration is 9% hydrogen peroxide solution, black shale particle Volume ratio with hydrogen peroxide is 1:18, and wherein black shale particle is 100 cm3, in order not to destroy fossil, it is necessary to be added 0.08 mol/L sodium carbonate liquor is as buffer solution, so that soak pH value is maintained at 8.5, solution temperature maintains 35 DEG C, reaction time 36h removes organic matter, so that black shale particle is tentatively scattered, then filter, and obtains pretreated black Color shale sample.
C, pretreated black shale sample is placed in container by wet screening, adds water, and the volume ratio of sample and water is 1: 18, then ebuillition of heated after object to be deposited scatters, is sieved with 200 mesh screens, then with tap water repeated flushing sieve in deposit, Then deposit in sieve is transferred in complementary tank, it is to be dried.
D, it dries, by the sample after wet screening in baking oven in 50 DEG C, toasts 45 h.
E, dry screen, the deposit sample after drying first crosses 40 mesh scalpings and obtains coarseness deposit sample, thin after 120 mesh Sieve obtains fine granularity deposit sample.
F, sample is chosen, Ostracods are concentrated mainly in fine granularity deposit sample, but coarseness deposit sample sometimes On also have a large amount of distributions, therefore two granularities are intended to choose sample, when choosing sample, by fine granularity deposit sample and coarseness deposit sample This piles the strip of an identical thickness respectively, is divided into 8 parts by length, chooses sample and carries out under Stereo microscope, mirror decentralization Big multiple is 40 times.
G, identification and statistics, amplification factor is 120 times when identifying, statistics is converted by volume number, i.e. 1 volume 10 Body, entire 4 volume of sample, 40/sample of abundance, such as 1/16 volume contain 10 individuals, then entire sample abundance be 160/ Sample, the total abundance of deposit sample are equal to fine granularity deposit sample abundance and add coarseness deposit sample abundance.
Embodiment three, the method that Ostracode microfossil is extracted from black shale, including sampling, pretreatment, wet screening, baking Dry, dry screen chooses sample and identification and statistics, specific steps are as follows:
A, it samples, black shale is ground into particle, particle volume is 0.5 cm3。
B, it pre-processes, black shale particle is placed in container, addition concentration is 10% hydrogen peroxide solution, black shale The volume ratio of grain and hydrogen peroxide is 1:25, and wherein black shale particle is 150 cm3, in order not to destroy fossil, it is necessary to be added 0.1 mol/L sodium carbonate liquor is as buffer solution, so that soak pH value is maintained at 9, solution temperature maintains 40 DEG C, instead For for 24 hours, removal organic matter makes black shale particle tentatively scatter, then filters, obtains pretreated black shale between seasonable Sample.
C, pretreated black shale sample is placed in container by wet screening, adds water, and the volume ratio of sample and water is 1: 25, then ebuillition of heated after object to be deposited scatters, is sieved with 200 mesh screens, then with tap water repeated flushing sieve in deposit, Then deposit in sieve is transferred in complementary tank, it is to be dried.
D, it dries, by the sample after wet screening in baking oven in 60 DEG C, toasts 40 h.
E, dry screen, the deposit sample after drying first crosses 40 mesh scalpings and obtains coarseness deposit sample, thin after 120 mesh Sieve obtains fine granularity deposit sample.
F, sample is chosen, Ostracods are concentrated mainly in fine granularity deposit sample, but coarseness deposit sample sometimes On also have a large amount of distributions, therefore two granularities are intended to choose sample, when choosing sample, by fine granularity deposit sample and coarseness deposit sample This piles the strip of an identical thickness respectively, is divided into 16 parts by length, chooses sample and carries out under Stereo microscope, mirror decentralization Big multiple is 80 times.
G, identification and statistics, amplification factor is 180 times when identifying, statistics is converted by volume number, i.e. 1 volume 10 Body, entire 4 volume of sample, 40/sample of abundance, such as 1/16 volume contain 10 individuals, then entire sample abundance be 160/ Sample, the total abundance of deposit sample are equal to fine granularity deposit sample abundance and add coarseness deposit sample abundance.

Claims (2)

1. the method for extracting Ostracode microfossil from black shale, it is characterized in that: including sampling, pretreatment, wet screening, baking Dry, dry screen chooses sample and identification and statistics, specific steps are as follows:
A, it samples, black shale is ground into particle, particle volume is 0.2~0.5 cm3；
B, it pre-processes, black shale particle is placed in container, addition concentration is 8~10% hydrogen peroxide solutions, black shale particle Volume ratio with hydrogen peroxide is 1:10~25, in order not to destroy fossil, it is necessary to 0.05~0.1 mol/L sodium carbonate liquor be added As buffer solution, so that soak pH value is maintained at 8~9, solution temperature maintains 30~40 DEG C, and the reaction time is 24~ 48h removes organic matter, so that black shale particle is tentatively scattered, then filter, obtain pretreated black shale sample；
C, pretreated black shale sample is placed in container by wet screening, adds water, the volume ratio of sample and water be 1:10~ 25, then ebuillition of heated after object to be deposited scatters, is sieved with 200 mesh screens, then with tap water repeated flushing sieve in deposit, Then deposit in sieve is transferred in complementary tank, it is to be dried；
D, it dries, by the sample after wet screening in baking oven in 40~60 DEG C, toasts 40~50 h；
E, dry screen, the deposit sample after drying first cross 40 mesh scalpings and obtain coarseness deposit sample, obtain after 120 mesh dusting covers Obtain fine granularity deposit sample；
F, sample is chosen, Ostracods are concentrated mainly in fine granularity deposit sample, but sometimes on coarseness deposit sample There are a large amount of distributions, therefore two granularities are intended to choose sample, when choosing sample, by fine granularity deposit sample and coarseness deposit sample point The strip for not piling an identical thickness is divided into N parts by length, wherein N >=2, is chosen sample and is carried out under Stereo microscope, mirror Lower amplification factor is 20~80 times；
G, identification and statistics, amplification factor is 60 times either 120 times or 180 times when identifying, statistics is rolled over by volume number It calculates, i.e. 1 volume, 10 individuals, entire 4 volume of sample, 40/sample of abundance, the total abundance of deposit sample is deposited equal to fine granularity Object sample abundance adds coarseness deposit sample abundance.
2. the method for extracting Ostracode microfossil from black shale as described in claim 1, it is characterized in that: in step B In, black shale particle is no less than 50 cm3。
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CN109946134A (en) * 2019-03-22 2019-06-28 贵州大学 The separating and extracting process of organic clay complex in a kind of black shale

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CN110164512B (en) * 2019-05-28 2022-03-29 南华大学 Statistic method for abundance of mesomorphic fossil
CN113321529B (en) * 2021-05-12 2022-03-29 中国地质大学（武汉） Purification method and screening purification device for marine diatom fossil

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CN103293020A (en) * 2012-03-05 2013-09-11 中国石油化工股份有限公司 Step-by-step grading sampling method suitable for carbonatite sample
CN104897444A (en) * 2015-05-21 2015-09-09 桂林理工大学 Pretreatment method used before test of total rock components of basalt
CN104897704A (en) * 2015-06-23 2015-09-09 中国石油化工股份有限公司江汉油田分公司勘探开发研究院 Method for quantitatively analyzing components in shale

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109946134A (en) * 2019-03-22 2019-06-28 贵州大学 The separating and extracting process of organic clay complex in a kind of black shale
CN109946134B (en) * 2019-03-22 2021-11-30 贵州大学 Separation and extraction method of organic clay complex in black shale

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