CN106000506A - Observing experimental bath tank for movement of in-vivo intestinal smooth muscle - Google Patents
Observing experimental bath tank for movement of in-vivo intestinal smooth muscle Download PDFInfo
- Publication number
- CN106000506A CN106000506A CN201610622901.3A CN201610622901A CN106000506A CN 106000506 A CN106000506 A CN 106000506A CN 201610622901 A CN201610622901 A CN 201610622901A CN 106000506 A CN106000506 A CN 106000506A
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- smooth muscle
- tank
- inside groove
- intestinal smooth
- bath
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/02—Water baths; Sand baths; Air baths
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1811—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using electromagnetic induction heating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Devices For Medical Bathing And Washing (AREA)
Abstract
The invention aims to provide an observing experimental bath tank for movement of in-vivo intestinal smooth muscle. The observing experimental bath tank comprises a tank body, a plank and a heating device, wherein the tank body is composed of an inner tank and an outer tank; the tank body is arranged on the heating device; the plank is arranged between the lower surface at the bottom of the inner tank and the upper surface at the bottom of the outer tank; two symmetrical frog nail holes are formed at the bottom of the inner tank; Locke solution is contained in the inner tank; running water is contained in the outer tank; the inner tank is communicated with the exterior of the tank body through a draining port pipe; an outward draining port pipe is arranged on the outer tank; a programming controller which is connected with the heating device is arranged on the outer side wall of the tank body and is used for controlling the power supply and temperature of the heating device. The observing experimental bath tank for movement of in-vivo intestinal smooth muscle has the advantages of simple structure, novel and unique design, easiness in manufacturing, low cost, easiness in operation, good use effect, no oxygen supply device, investment-saving effect, reduced cost and obvious economic and social benefits.
Description
Technical field
The present invention relates to medical experiment instruction equipment, particularly a kind of at body intestinal smooth muscle movement observation experiment bath.
Background technology
At medical domain, for the experimental teaching of intestinal smooth muscle movement observation, it is usually the method using constant temperature smooth muscle groove in vitro observation in vitro Intestine in Rabbits smooth muscle movement.But some colleges is because condition limits, and does not has constant temperature smooth muscle groove, does not more have oxygen supply equipment, so this instructional item cannot be carried out to student.Some experimental teaching materials or teaching unit are the exercise intensitys using macroscopic method to determine small intestinal, and this method is the most objective and accurate.
In scientific research and experimental teaching, using the motion at body observer's rabbit intestinal smooth muscle, pulled out by small intestinal external, be fixed on frog board, medicine and reagent directly drips on intestinal tube surface.So, the blood circulation making full use of rabbit itself maintains body temperature and the nutrition supply of small intestinal, has farthest ensured the physiological function of small intestinal.
But, due to the problem on experimental facilities, have the following disadvantages in actual experiment: 1, medicine and reagent drips on intestinal tube surface, and reagent is lost soon, the time being present in intestinal tube surface is shorter, so the change of intestinal motility curve typically only has about 2 waveforms.Although 2 have blood supply to ensure the relative constancy of small intestinal temperature, but small intestinal be after all be drawn out external, now the temperature of small intestinal can only be constant at about 30 DEG C ± 2 DEG C, certainly will cause certain impact relative to internal 38 DEG C of differences 8 DEG C, the so physiological function on small intestinal.3, small intestinal is exposed to external meeting for a long time affects its motor function to a certain extent because of being dried.
Summary of the invention
For above-mentioned situation, for overcoming the defect of existing equipment, the purpose of the present invention is just to provide a kind of at body intestinal smooth muscle movement observation experiment bath, can effectively solve: the blood circulation that can make full use of rabbit itself maintains body temperature and nutrition supply, the normal physiological function of rabbit can be ensured most possibly the most again by the temperature incubation function of experiment bath and the function accommodating locke solution；By experiment bath to the external fixing function at body small intestinal, make biological information acquisition system can the tension variation of perception intestinal smooth muscle delicately, avoid breathing the interference problem to intestinal motility curve record simultaneously.
The technical scheme that the present invention solves is, including cell body, plank and heater, cell body is to be made up of inside groove and water jacket, cell body is placed in above heater, below bottom inside groove and outer slot bottom above between be equipped with plank, two symmetrical frog nail are had bottom inside groove, inside groove is built with locke solution, water jacket is interlinked to the outside with cell body through leakage fluid dram pipe built with tap water, inside groove, and water jacket has outside discharge outlet pipe, equipped with the cyclelog being connected with heater on the lateral surface of cell body, for controlling power supply and the temperature of heater.
Present configuration is simple, novel unique, easily manufactures, low cost, easily operates, and using effect is good, it is not necessary to oxygen supply equipment, investment reduction, reduces cost, has significant economic and social benefit.
Accompanying drawing explanation
Fig. 1 is the structural front view of the present invention；
Fig. 2 is the top view of cell body part of the present invention.
Detailed description of the invention
Below in conjunction with accompanying drawing, the detailed description of the invention of the present invention is elaborated:
By Fig. 1, shown in Fig. 2, the present invention includes cell body, plank 7 and heater 8, cell body is to be made up of inside groove 5 and water jacket 4, cell body is placed in above heater 8, it is equipped with plank 7 between above below bottom inside groove 5 and bottom water jacket 4, two symmetrical frog nail 3 are had bottom inside groove 5, inside groove 5 is built with locke solution 11, water jacket 4 is built with tap water 12, inside groove 5 is interlinked to the outside with cell body through leakage fluid dram pipe 1, outside discharge outlet pipe 2 is had on water jacket 4, equipped with the cyclelog 6 being connected with heater 8 on the lateral surface of cell body, for controlling power supply and the temperature of heater 8.
In order to ensure using effect and easy to use, described heater 8 is electrical heating wire stove or electromagnetic oven.
Described cell body is long cube or ellipse, water jacket 4 length × width × heights=9cm × 6cm × 5cm, inside groove 5 length × width × heights=7cm × 4cm × 3cm.
Described plank 7 is identical with the shape and size bottom inside groove 5, and thickness is 1-2cm.
A diameter of 2 μm of described frog nail 3, the distance between two frog nail 3 is 5cm.
Described leakage fluid dram pipe 1, discharge outlet pipe 2 diameter are 0.5cm, the discharge opeing mouth of pipe 1 controls valve 1-1 equipped with first, drain pipe port 2 controls valve 2-1 equipped with second.
The service condition of the present invention is, first will fill tap water in water jacket, opens heater, by tap water heating to 38 DEG C.The small intestinal 15 of one section of about 5cm length is external from the pull-out of rabbit abdominal cavity, it is placed in inside groove, it is fixed on the plank bottom inside groove by two frog nail bottom inside groove with two frog nails, with about 20 centimeters of long connecting lines 10, spaced winding small intestinal 15 1 weeks at two frog nails, after ligation, small intestinal 15 is sling gently, be connected with the tonotransducer 9 on bracing frame.38 DEG C of locke solutions are injected in inside groove.Described bracing frame is to be made up of with bend pipe 14 base 13.
Tonotransducer accesses on 1 passage of BL-420F system.Opening computer, start BL-420F System of organism signal, click on " input signal " menu, 1 channel selecting " tension force " at menu bar, click tools hurdle " starts " icon, enters experimental project.
Trace autorhythmicity shrinkage curve and trace the autorhythmicity shrinkage curve of one section of intestinal smooth muscle, note baseline values.At the curve movement of body intestinal smooth muscle owing to being affected by breathing, so there is fist-order wave and secondary wave.The baseline of shrinkage curve raises, amplitude increases, and represents that intestinal smooth muscle tonicity raises；On the contrary, the baseline of shrinkage curve moves down, and amplitude declines, and represents that tonicity reduces.The rhythm and pace of moving things of shrinkage curve, waveform, frequency and amplitude should be observed simultaneously.
The present invention can be effectively used for doing following laboratory observation:
1 material and method
1.1 experiment material
1.1.1 laboratory animal
Rabbit.
1.1.2 experiment equipment BL-420F biological functional system (Chengdu TME Technology Co., Ltd.).Computer, mammal operating theater instruments 1 overlaps (surgical scissors, mosquito forceps, tweezers etc.), screw clamp, iron stand, glass minute hand, tonotransducer (9), beaker, thermometer, frog board, and the frog is followed closely, connecting line (10), tests bath.
1.1.3 reagent 20% urethanes, locke solution (11), 1:10000 epinephrine solution, 1:10000 acetylcholine solution, 1:10000 atropine.
1.2 experimental technique
1.2.1 anaesthetize and perform the operation 20% urethanes auricular vein injecting anesthetic rabbit, dorsal position is fixed on rabbit platform, open abdominal cavity, find one section of small intestinal of rear under stomach and pull it abdominal cavity, by the above-mentioned service condition be given, small intestinal tension is tested.
1.2.2 observation item
The first fixing means: bath need not be tested
1. the effect of temperature
With dropper to dripping 20 DEG C of locke solutions on body small intestinal surface, observation index is ibid；It is replaced with 40 DEG C of locke solutions again, observes the change of intestinal smooth muscle shrinkage curve.Finally it is replaced with 30 DEG C of locke solutions more normal to the shrinkage curve recovery of intestinal smooth muscle.
2. the effect of acetylcholine
With dropper at body small intestinal surface dropping 1:10000 acetylcholine solution 2～4, observation index is ibid.After observing obvious effect, immediately with normal saline dilution and wash the acetylcholine on body small intestinal surface.
The most atropinic effect
With dropper at body small intestinal surface dropping 1:10000 atropine 2～4, then dropping 1:10000 acetylcholine solution 2～4 rapidly, observation index is ibid.After observing obvious effect, immediately with normal saline dilution and wash the atropine on body small intestinal surface and acetylcholine.
The most adrenergic effect
With dropper at body small intestinal surface dropping 1:10000 epinephrine solution 2～4, observation index is ibid.After observing obvious effect, immediately with normal saline dilution and wash the epinephrine on body small intestinal surface.
The second fixing means: with experiment bath
1. the effect of temperature
Locke solution in bath is replaced with 20 DEG C of locke solutions, and observation index is ibid.It is replaced with 40 DEG C of locke solutions again, observes the change of intestinal smooth muscle shrinkage curve.Finally it is replaced with 38 DEG C of locke solutions more normal to the shrinkage curve recovery of intestinal smooth muscle.
2. the effect of acetylcholine
Dripping 1:10000 acetylcholine solution 2 in bath with dropper, observation index is ibid.After observing obvious effect, release the locke solution containing acetylcholine from bath outlet pipe immediately, add preprepared 38 DEG C of locke solutions.
The most atropinic effect
Repeat to change 2～3 38 DEG C of locke solutions, make the acetylcholine of residual reach concentration ineffective.When the shrinkage curve of intestinal smooth muscle recovers to control level, instilling 1:10000 atropine 2～4 with dropper in bath, observation index is ibid.After observing obvious effect, adding 1:10 000 acetylcholine solution 2, the shrinkage curve observing intestinal smooth muscle has unchanged.
The most adrenergic effect
Changing 2～3 38 DEG C of locke solutions, when the shrinkage curve of intestinal smooth muscle recovers to control level, add 1:10000 epinephrine solution 2 in bath, observation index is ibid.
2 experimental results
After dropping epinephrine, the baseline of shrinkage curve moves down, and the amplitude shunk reduces.On the contrary, after dropping acetylcholine, the baseline of shrinkage curve moves, and the amplitude shunk increases.Other observation item has been similarly obtained intended experimental result.
3 conclusions
Observe the experiment of intestinal smooth muscle motion at body, all observation items have obtained intended experimental result.
If but fix with the first and observational technique, there is problems in that and 1. directly reagent dropwise is lost soon at intestinal tube surface, reagent, the time being present in intestinal tube surface is shorter, so the change of curve movement typically only has about 2 waveforms.2. after small intestinal pull-out is external, because there being blood supply, so small intestinal temperature relative constancy (about at 30 DEG C ± 2 DEG C) can be maintained, but with small intestinal temperature difference about 8 DEG C in regular, necessarily disturbed so just having pulled out external intestinal motility function, needing certain wait adaptive time, small intestinal could recover proper motion function；3. small intestinal is exposed to external meeting for a long time affects its motor function to a certain extent because of being dried.
In order to eliminate or reduce above-mentioned drawback, can fix with the second and observational technique, be placed in by small intestinal in the experiment bath of present invention design, as long as medicine and reagent retains in experiment bath, experiment effect the most persistently occurs.Eliminate temperature the most simultaneously and be dried the interference to intestinal motility function, without oxygen supply equipment.
Experimental applications proves, the present invention has simple in construction, easy to operate, low cost, and experiment effect is good, accurately, improves experiment teaching effect and quality of instruction, objective, has good actual application value, and economic and social benefit is notable.
Claims (6)
1. test bath at body intestinal smooth muscle movement observation for one kind, including cell body, plank (7) and heater (8), it is characterized in that: cell body is to be made up of inside groove (5) and water jacket (4), cell body is placed in heater (8) above, inside groove (5) bottom below with water jacket (4) bottom above between be equipped with plank (7), two symmetrical frog nail (3) are arranged at inside groove (5) bottom, inside groove (5) is built with locke solution (11), water jacket (4) is built with tap water (12), inside groove (5) is interlinked to the outside with cell body through leakage fluid dram pipe (1), outside discharge outlet pipe (2) is had on water jacket (4), equipped with the cyclelog (6) being connected with heater (8) on the lateral surface of cell body.
The most according to claim 1 at body intestinal smooth muscle movement observation experiment bath, it is characterized in that: described heater (8) is electrical heating wire stove or electromagnetic oven.
The most according to claim 1 at body intestinal smooth muscle movement observation experiment bath, it is characterized in that: described cell body is long cube or ellipse, water jacket 4 length × width × heights=9cm × 6cm × 5cm, inside groove 5 length × width × heights=7cm × 4cm × 3cm.
The most according to claim 1 at body intestinal smooth muscle movement observation experiment bath, it is characterized in that: described plank (7) is identical with the shape and size of inside groove (5) bottom, and thickness is 1-2cm.
The most according to claim 1 at body intestinal smooth muscle movement observation experiment bath, it is characterized in that: described a diameter of 2 μm of frog nail (3), the distance between two frog nail (3) is 5cm.
The most according to claim 1 at body intestinal smooth muscle movement observation experiment bath, it is characterized in that: described leakage fluid dram pipe (1), discharge outlet pipe (2) diameter are 0.5cm, control valve (1-1) equipped with first on the discharge opeing mouth of pipe (1), drain pipe port (2) controls valve (2-1) equipped with second.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610622901.3A CN106000506A (en) | 2016-08-02 | 2016-08-02 | Observing experimental bath tank for movement of in-vivo intestinal smooth muscle |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610622901.3A CN106000506A (en) | 2016-08-02 | 2016-08-02 | Observing experimental bath tank for movement of in-vivo intestinal smooth muscle |
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| CN106000506A true CN106000506A (en) | 2016-10-12 |
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| CN201610622901.3A Pending CN106000506A (en) | 2016-08-02 | 2016-08-02 | Observing experimental bath tank for movement of in-vivo intestinal smooth muscle |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110186922A (en) * | 2019-06-25 | 2019-08-30 | 西安交通大学医学院第一附属医院 | A kind of experimental provision and method monitoring colon Migtating motor complex |
Citations (3)
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| US4054416A (en) * | 1976-08-11 | 1977-10-18 | Secretary Of State For Social Services | Apparatus for use in investigating specimens |
| CN103537331A (en) * | 2013-10-14 | 2014-01-29 | 北京工业大学 | High-polymer nano-grade indentation constant-temperature water-bath sample bench and related experiment method |
| CN104549598A (en) * | 2013-10-18 | 2015-04-29 | 西安敏海电子科技有限公司 | Thermostatic water bath provided with heating piece fixing device |
-
2016
- 2016-08-02 CN CN201610622901.3A patent/CN106000506A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4054416A (en) * | 1976-08-11 | 1977-10-18 | Secretary Of State For Social Services | Apparatus for use in investigating specimens |
| CN103537331A (en) * | 2013-10-14 | 2014-01-29 | 北京工业大学 | High-polymer nano-grade indentation constant-temperature water-bath sample bench and related experiment method |
| CN104549598A (en) * | 2013-10-18 | 2015-04-29 | 西安敏海电子科技有限公司 | Thermostatic water bath provided with heating piece fixing device |
Non-Patent Citations (3)
| Title |
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| 余良主等: "《医学机能学实验指导》", 31 January 2016, 武汉：华中科技大学出版社 * |
| 王丙云: "《动物机能学实验数据》", 31 December 2014, 广州：华南理工大学出版社 * |
| 解景田等: "《生理学实验》", 31 January 2009, 北京：高等教育出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110186922A (en) * | 2019-06-25 | 2019-08-30 | 西安交通大学医学院第一附属医院 | A kind of experimental provision and method monitoring colon Migtating motor complex |
| CN110186922B (en) * | 2019-06-25 | 2021-07-23 | 西安交通大学医学院第一附属医院 | Experimental device and method for monitoring colon transitional compound movement |
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Application publication date: 20161012 |
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