CN105995017A - Seed pumpkin and corn stalk fermented fodder and preparation method thereof - Google Patents
Seed pumpkin and corn stalk fermented fodder and preparation method thereof Download PDFInfo
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- CN105995017A CN105995017A CN201610487117.6A CN201610487117A CN105995017A CN 105995017 A CN105995017 A CN 105995017A CN 201610487117 A CN201610487117 A CN 201610487117A CN 105995017 A CN105995017 A CN 105995017A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses seed pumpkin and corn stalk fermented fodder and a preparation method thereof, belongs to the technical field of fermented fodder, and aims at solving the technical problem that seed pumpkins are high in moisture content and difficult to store. The fermented fodder is prepared from 4-9 parts by mass of filiform seed pumpkins and 6 parts by mass of stalk powder. The method comprises the following steps that seed pumpkin pulp is processed to be filiform to obtain the filiform seed pumpkins, the corn stalk is smashed into stalk powder, the filiform seed pumpkins are mixed with the stalk powder, water is added, mixing is conducted to be uniform, the mixture is rapidly put into a fermentation breathing bag provided with a one-way air valve for sealing and subjected to indoor dark storage for 45 d to 60 d, and a finished product is obtained, wherein the one-way air valve is used for discharging air out of the fermentation breathing bag. According to the seed pumpkin and corn stalk fermented fodder and the preparation method thereof, the high moisture content of the seed pumpkins is balanced through the corn stalk, the defect that the seed pumpkins are not suitable for storage is overcome, the waste pulp of the seed pumpkins and the corn stalk are turned into wealth, the resource utilization rate of the seed pumpkins and the corn stalk is increased, safe storage is achieved, the coarse fodder resource of ruminants is effectively developed, and the environment is protected.
Description
Technical field
The invention belongs to the technical field of fermented feed；Be specifically related to a kind of seed-use pumpkin and corn straw fermented feed and
Preparation method.
Background technology
Seed-use pumpkin is the plant that Cucurbitaceae seed-use pumpkin belongs to, and it belongs to annual herbaceous plant, is again seed-use pumpkin
Seed.The topmost effect of seed-use pumpkin is just to provide Seed Pumpkin seed, and seed is many, and single rate is the highest.
Owing to seed-use pumpkin is as other gourd, fruit and vegetables, in a period of time after harvesting, can keep original just
Being frequently grown state, so, as time goes by, the nutritional labeling in seed-use pumpkin can be along with moisture transpiration, respiratory metabolism
Process consumes a part, reduces the content of the nutrient substance such as the protein in seed-use pumpkin, saccharide.Therefore, adopt at seed-use pumpkin
After plucking, it has to the problem considering storage.
The main purpose of plantation seed-use pumpkin is to obtain Seed Pumpkin seed, and its sarcocarp major part is simply discarded, and
Seed-use pumpkin sarcocarp itself has the highest nutrition and medical value.Area, the Three Eastern Provinces, especially Heilongjiangdistrict belong to seed and use
One of important producing region of Fructus Cucurbitae moschatae, owing to seed-use pumpkin moisture is high, it is difficult to storage, utilizes extremely limited to sarcocarp resource,
And environment is also had a great impact.
Summary of the invention
The invention solves the problems that the high-leveled and difficult technical problem to store of seed-use pumpkin moisture；And provide a kind of seed-use pumpkin
With corn straw fermented feed and preparation method.
For solving above-mentioned technical problem, in the present invention fermented feed described in seed-use pumpkin and corn straw fermented feed be by
Seed-use pumpkin silk and powder of straw are by for (4～9): the mass ratio of 6 is made.
A diameter of (0.20～0.40) cm of described seed-use pumpkin silk.
A length of (2～3) cm of described corn stalk powder.
In the present invention, the preparation method of a kind of seed-use pumpkin and corn straw fermented feed is carried out in the steps below: will
Seed-use pumpkin melon meat is processed into thread acquisition seed-use pumpkin silk, and corn straw smashing obtains powder of straw, then by seed-use pumpkin silk
Add water after mixing with powder of straw mixing, is loaded on rapidly rear in being provided with the fermentation breathing bag of Non-return air valve sealing, keeps away as indoor
Light stores 45～60d, and described Non-return air valve is by the gas discharging in fermentation breathing bag.
The moisture adding water management solid content is 65%～75%.
Indoor temperature controls lucifuge at 20～25 DEG C and places.
Described fermentation breathing bag is long 20cm, wide 35cm, the feed fermentation breathing bag of diameter 15cm.
Seed-use pumpkin is mixed after microbial prepared by the fermented feed of the present invention with corn straw, is put down by corn straw
Weighing apparatus seed seed-use pumpkin high-moisture solves the shortcoming that seed-use pumpkin should not be stored, and seed-use pumpkin is discarded sarcocarp and corn straw
Turn waste into wealth, improve seed-use pumpkin and maize straw resource utilization rate, safe storage, effective exploitation ruminant roughage resource
With protection environment.
Under conditions of the fermented feed of the present invention ensures to seal, more than 5 years can be stored.
Accompanying drawing explanation
Fig. 1 is the impact that Fructus Cucurbitae moschatae and micro preservation of corn stalk pH are changed by different in moisture content；
Fig. 2 is the impact that Fructus Cucurbitae moschatae and micro preservation of corn stalk LAB are changed by different in moisture content；
Fig. 3 is the impact that Fructus Cucurbitae moschatae and micro preservation of corn stalk LA are changed by different in moisture content；
Fig. 4 is the impact that Fructus Cucurbitae moschatae and micro preservation of corn stalk AN are changed by different in moisture content；
Fig. 5 is the impact that Fructus Cucurbitae moschatae and micro preservation of corn stalk AA are changed by different in moisture content；
Fig. 6 is that different grain size Fructus Cucurbitae moschatae mixes, with corn straw, the impact store pH change；
Fig. 7 is that different grain size Fructus Cucurbitae moschatae mixes, with corn straw, the impact store LAB change；
Fig. 8 is that different grain size Fructus Cucurbitae moschatae mixes, with corn straw, the impact store LA change；
Fig. 9 is that different grain size Fructus Cucurbitae moschatae mixes, with corn straw, the impact store AN change；
Figure 10 is that different grain size Fructus Cucurbitae moschatae mixes, with corn straw, the impact store AA change.
Detailed description of the invention
Detailed description of the invention one: in present embodiment fermented feed described in seed-use pumpkin and corn straw fermented feed be by
Seed-use pumpkin silk and powder of straw are made by the mass ratio for 1:1, a diameter of 0.20cm of described seed-use pumpkin silk, described Semen Maydis
The a length of 2cm of powder of straw；Its preparation method is that Fructus Cucurbitae moschatae melon meat is processed into thread acquisition seed-use pumpkin silk, corn straw smashing
Obtaining powder of straw, add water after then seed-use pumpkin silk and powder of straw being mixed mixing, and the moisture controlling solid content is 70%,
Being loaded on rapidly in the fermentation breathing bag being provided with Non-return air valve, compacting also carries out application of vacuum with vacuum packing machine immediately and moulds
Envelope, stores 45d as the indoor lucifuge that room temperature is 25 DEG C, and the gas that earlier fermentation (3-5d) can be produced by described Non-return air valve is from sending out
Discharge in ferment breathing bag.
Described fermentation breathing bag is long 20cm, wide 35cm, the feed fermentation breathing bag of diameter 15cm.
Use following verification experimental verification invention effect:
Test one, different proportion seed-use pumpkin and the corn straw impact on mixed storage effect
The ratio of test totally 7 process groups, seed-use pumpkin and corn straw is respectively 6:4,5:5,4:6；Mixed storage raw water
Content is divided to be respectively 65%, 70% and 75%.By seed-use pumpkin (a diameter of 0.2cm filament), corn straw (a length of 2cm) and
The water added, according to the ratio mixing calculated, is loaded on rapidly after mix homogeneously in feed fermentation breathing bag, compacting, and uses immediately
Vacuum packing machine carries out application of vacuum and plastic packaging.20～25 DEG C disposed within of feed fermentation breathing bag keeps in Dark Place.Respectively 0d,
5d, 10d, 20d, 30d, 45d and 60d, 7 point in time sampling carry out subjective appreciation, measure pH, AN, LAB, LA and AA, and measure
NDF, ADF and CP content of the gathered sample of 60d.Each of the above processes 4 repetitions；EXPERIMENTAL DESIGN is shown in Table 1.
Table 1 EXPERIMENTAL DESIGN
Feedstuff as reference, is divided into one-level (excellent with " German agriculture association ensilage sensory evaluation scores standard " by sensory evaluation
Good) (20-16 divides), two grades (fair) (15-10 divides), three grades (medium) (9-5 divides) and level Four (corrupt) (4-0 divides) 4 grades.
Crude protein (CP)
CP is multiplied by 6.25 by total nitrogen and calculates, and total nitrogen uses FOSS full-automatic Kjeldahl determination device to measure.
Ammoniacal nitrogen (AN) measures and uses phenol-sodium hypochlorite colorimetry.
Neutral detergent fiber (NDF) uses ANKOM fibre analyser to measure.
The mensuration of acid detergent fiber (ADF) measures with NDF.
PH measures: weigh microbiological fermented feed 20g, loads in tool plug triangular flask, adds 180ml distilled water, at the refrigerator of 4 DEG C
Standing 24h, treat that it fully soaks, collect filtrate by four layers of filtered through gauze, filtrate measures with electronics acidometer.
The sum of lactic acid bacteria (LAB) measures and measures according to state food safety criterion (GB4789.2-2010) method.
Lactic acid (LA) uses high performance liquid chromatography Waters600 to measure.
Acetic acid (AA) uses Shimadzu GC-2010 gas chromatograph for determination.
Test data uses the general linear model in SPSS19.0 software to carry out 3 × 2 two-way analysis of variances, and uses
Duncan method carries out multiple comparisons, using P ＜ 0.05 as significance of difference criterion.Result is with mean+SD table
Show.
Different proportion seed-use pumpkin and corn straw mix the sensory evaluation of storage
After microbial terminates, the sense organ of microbial quality is evaluated, with reference to German agriculture association (Deutche
Landwirtschafts Gesellschaft) ensiling quality sense organ standards of grading carry out mark (being shown in Table 2).Gas from microbial material
Taste, color and luster, 3 outside indexs directly perceived of quality are marked.
Table 2 different proportion seed-use pumpkin and corn straw mix the subjective appreciation of storage
As shown in table 2, being 70% in moisture, the test group of 3 ratios belongs to first-class ensilage；75%
Under moisture, ratio be the sensory evaluation scores of the test group of 4:6 be 15, belong to second-class quality, remain two ratios and belong to first-class blue or green
Storage feedstuff.
Different proportion seed-use pumpkin and corn straw mix store on pH value to affect result as shown in table 3:
Table 3 different proportion seed-use pumpkin and corn straw mix the storage impact on pH value
Note: * represents 70% moisture group and the significant difference of 75% moisture group, P < 0.05 under same ratio.a-cDifferent letters
Represent the significant difference of different proportion in 70% moisture, P < 0.05.A-CIn different letter representation 75% moisture, different proportion is aobvious
Write difference, P < 0.05.
As shown in table 3, under 75% moisture of 0d, 5d, 10d and 45d time point, difference between the test group of different proportion
Significantly (P < 0.05).At 0d and 20d time point, the pH of 70% different proportion is below the moisture of the 75% of corresponding ratio.Except
5d time point, the test group significant difference (P < 0.05) of different proportion under 70% moisture of remaining time point.In 20d～the 60d phase
Between each time point, two corresponding ratios of ratio different in moisture of 4:6 and 5:5 the equal significant difference of pH (P <
0.05).It is also seen that the pH of each group is all gradually lowered along with the carrying out of microbial from table.Additionally, at 5d, 30d, 45d and 60d
Time point, seed-use pumpkin demonstrates obvious interaction effect (P < 0.05) with mixed proportion and the moisture of straw.
It is as shown in table 4 on the impact of LAB number that different proportion seed-use pumpkin and corn straw mix storage:
Table 4 different proportion seed-use pumpkin and corn straw mix the storage impact (cfu/ml) on LAB number
Note: * represents 70% moisture group and the significant difference of 75% moisture group, P < 0.05 under same ratio.a-cDifferent letters
Represent the significant difference of different proportion in 70% moisture, P < 0.05.A-CIn different letter representation 75% moisture, different proportion is aobvious
Write difference, P < 0.05.
As seen from Table 4, during whole microbial, respectively the LAB of group is all in first raising the trend reduced afterwards, and rear
Phase, decline tends towards stability.Under the moisture of 70%, except 0d and 10d time point, the test group difference of three ratios does not shows
Write (P > 0.05).At microbial later stage (after 30d), moisture be the different proportion of 75% test group significant difference (P <
0.05).At 0d time point, significant difference (P < 0.05) between 5:5 and 6:4 corresponding ratio different in moisture, at 60d time point,
Significant difference (P < 0.05) between 4:6 corresponding ratio different in moisture.At 10d and 60d time point, seed-use pumpkin is mixed with straw
Composition and division in a proportion example and moisture demonstrate obvious interaction effect (P < 0.05).Different proportion seed-use pumpkin and the mixed storage of corn straw are right
The impact of LA content is as shown in table 5:
Table 5 different proportion seed-use pumpkin and corn straw mix the storage impact (%) on LA content
Note: * represents 70% moisture group and the significant difference of 75% moisture group, P < 0.05 under same ratio.a-cDifferent letters
Represent the significant difference of different proportion in 70% moisture, P < 0.05.A-CIn different letter representation 75% moisture, different proportion is aobvious
Write difference, P < 0.05.
As shown in table 5, at 0d and 60d time point, the LA of two ratios of 5:5 and 6:4 and the different water of corresponding ratio
The significant difference (P < 0.05) divided.At 0d and 30d time point, the LA value significant difference of each test group of 70% moisture different proportion
(P<0.05).At 45d and 60d time point, the 4:6 of 70% moisture is notable (P < 0.05) with other two proportional difference, but its two
Between person, difference is not notable (P > 0.05).Under 20d, 45d and 60d time point, 75% moisture, each group difference of different proportion does not shows
Write (P > 0.05).Significant difference (P < 0.05) between the different in moisture under 0d, 30d, 45d and 60d time point, 5:5 ratio.By table
It is also seen that except 20d time point, with mixed proportion and the moisture of straw, seed-use pumpkin demonstrates that obvious interaction is made
With (P < 0.05).It is as shown in table 6 on the impact of CP and NDF, ADF content that different proportion seed-use pumpkin and corn straw mix storage:
Table 6 different proportion seed-use pumpkin and the mixed storage of corn straw are on CP and NDF, the impact of ADF content (air bells dry basis)
Note: * represents 70% moisture group and the significant difference of 75% moisture group, P < 0.05 under same ratio.a-cDifferent letters
Represent the significant difference of different proportion in 70% moisture, P < 0.05.A-CIn different letter representation 75% moisture, different proportion is aobvious
Write difference, P < 0.05.
As shown in table 6, under 70% and 75% moisture, the albumen between seed-use pumpkin and straw ratio 5:5 and 4:6,6:4 contains
Amount difference is not notable (P > 0.05), and under 4:6 and 5:5 ratio, between the test group of the different in moisture of corresponding ratio, protein content is poor
Different not notable (P > 0.05).Under 70% moisture, NDF and the ADF content difference of 4:6 and two other ratio notable (P <
, and be above other two groups 0.05).Under 5:5 ratio, NDF and the ADF content difference of different in moisture is notable (P < 0.05),
75% process group is higher than 70% process group.It is also seen that the mixed proportion of seed-use pumpkin and straw and water content are to ADF from table
Content has obvious interaction effect (P < 0.05).Different proportion seed-use pumpkin and corn straw mix stores the impact on AN content such as
Shown in table 7:
Table 7 different proportion seed-use pumpkin and corn straw mix the storage impact (%) on AN content
Note: * represents 70% moisture group and the significant difference of 75% moisture group, P < 0.05 under same ratio.a-cDifferent letters
Represent the significant difference of different proportion in 70% moisture, P < 0.05.A-CIn different letter representation 75% moisture, different proportion is aobvious
Write difference, P < 0.05.
As can be seen from Table 7,30d time point, each ratio under 70% moisture from test group difference notable (P >
0.05), at 0d and 60d time point, the AN of the test group of the corresponding ratio of different in moisture of tri-ratios of 4:6,5:5,6:4 contains
Amount significant difference (P < 0.05).4:6 Yu 5:5 under 60d time point, 75% moisture, the AN content difference of 6:4 notable (P <
, and AN content that ratio is 5:5 is less than two other ratio 0.05).By table 3-6 it is also seen that except 20d and the 30d time
Point, at remaining each time point, seed-use pumpkin all demonstrates obvious interaction effect with mixed proportion and the moisture of straw
(P<0.05)。
It is as shown in table 8 on the impact of AA content that different proportion seed-use pumpkin and corn straw mix storage
Table 8 different proportion seed-use pumpkin and corn straw mix the storage impact (%) on AA content
Note: * represents 70% moisture group and the significant difference of 75% moisture group, P < 0.05 under same ratio.a-cDifferent letters
Represent the significant difference of different proportion in 70% moisture, P < 0.05.A-CIn different letter representation 75% moisture, different proportion is aobvious
Write difference, P < 0.05.
As shown in table 8, under 30d time point, 70% moisture, ratio is significant difference (P between 6:4 and 5:5,4:6 test group
<0.05).In the whole microbial phase, the AA content of each group all in downward trend after first raising, declines in the microbial later stage (after 30d)
Trend is mild.From table 3-7 it is also seen that at each time point, seed-use pumpkin and the mixed proportion of straw and moisture are without bright
Aobvious interaction effect (P > 0.05).
Seed-use pumpkin and corn straw are mixed the impact of storage effect by test two, different in moisture
Test totally 3 process groups, microbial thing moisture is respectively 65%, 70% and 75%, each process 4 repetition.
Seed-use pumpkin (a diameter of 0.2cm filament) and corn straw (a length of 2cm) mix with the ratio of 1:1, after mix homogeneously, load
In feed fermentation breathing bag, vacuum, 20～25 DEG C disposed within keep in Dark Place.Respectively at 0d, 10d, 30d and 60d, 4 times
Point sampling carries out subjective appreciation, measures pH, AN, LAB, LA and AA, and measures NDF, ADF and CP of the gathered sample of 0d.Test
Design is shown in Table 9.
Table 9 EXPERIMENTAL DESIGN
Test data uses SPSS19.0 software that data carry out one factor analysis of variance, and carries out multiple by Duncan method
Relatively, using P ＜ 0.05 as significance of difference criterion.Result represents with mean+SD.
The impact that Fructus Cucurbitae moschatae and corn straw are mixed storage sensory evaluation by different in moisture is as shown in table 10:
Fructus Cucurbitae moschatae and corn straw are mixed the impact of storage subjective appreciation by table 10 different in moisture
As seen from Table 10 moisture be 70% the sensory evaluation scores of process group be up to 19 points, moisture is 75%
Process group sensory evaluation scores be 17 points, both of which belongs to one-level ensilage (excellent).And the process group that moisture is 65%
15 points, sensory evaluation scores position, belongs to 2 grades of ensilages (fair).
Fructus Cucurbitae moschatae and corn straw are mixed the impact of storage pH value as it is shown in figure 1, in whole process of the test by different in moisture, each
The pH of test group is all in the trend being gradually reduced, and along with the rising of moisture content, the pH of each test group is in reducing trend, respectively
Group difference is not notable (P > 0.05).The pH size order of microbial later stage each test group is followed successively by 65% > 70% > 75%.
Different in moisture Fructus Cucurbitae moschatae and corn straw are mixed storage LAB number impact as shown in table 11 and Fig. 2, at 10d time point, respectively
The LAB number of test group is higher than 0d time point, and the LAB number of the most each test group is in being gradually reduced trend, and tends to flat after 30d
Surely.At 0d time point, water content is the test group (P < 0.05) that the LAB number of the test group of 65% is substantially less than 70%, 75%.From
Table 11 it can also be seen that at 30d and 60d time point, moisture be the LAB number of the test group of 70% be all remarkably higher than 65%,
The test group (P < 0.05) of 75%
Fructus Cucurbitae moschatae and corn straw are mixed the impact (cfu/ml) of storage LAB number by table 11 different in moisture
Note: same column letter shoulder mark difference represents significant difference (P < 0.05).
Different in moisture Fructus Cucurbitae moschatae and corn straw are mixed storage LA content impact as shown in table 12 and Fig. 3,0d time point, aqueous
Amount be 65% test group LA content be significantly higher than 70% and 75% test group (P < 0.05).Along with the carrying out of microbial, each group
LA content in the trend gradually risen.From table 12 it can also be seen that at 10d time point, significant difference between each group (P <
0.05).At 30d and 60d time point, moisture is the test of the test group LA content all substantially less than 65% and 70% of 75%
Group (P < 0.05).
Fructus Cucurbitae moschatae and corn straw are mixed the impact (%) of storage LA content by table 12 different in moisture
Different in moisture Fructus Cucurbitae moschatae and corn straw are mixed storage CP and NDF, ADF content impact as shown in table 13:
Fructus Cucurbitae moschatae and corn straw are mixed storage CP and NDF, the impact of ADF content by table 13 different in moisture
As can be seen from Table 13, moisture be 70% the CP content of test group be significantly higher than 65% and 75% (P <
0.05), its NDF content is substantially less than 65% and 75% (P < 0.05).It is also seen that water content is the test group of 75% from table
ADF content the highest, the ADF content of 65% takes second place, and the ADF content of 70% is minimum.
Different in moisture Fructus Cucurbitae moschatae and corn straw are mixed storage AN content impact as shown in table 14 and Fig. 4, at 0d time point, contain
The water yield is that the AN content of the process group of 75% is substantially less than 65% and 70% (P < 0.05), at 10d, 30d, 60d time point, and each group
Between difference notable (P > 0.05).It is also seen that the AN content of each group is all in first raising the trend reduced afterwards from table 14.
Fructus Cucurbitae moschatae and corn straw are mixed the impact (%) of storage AN content by table 14 different in moisture
Note: same column letter shoulder mark difference represents significant difference (P < 0.05).
Different in moisture Fructus Cucurbitae moschatae and corn straw are mixed storage AA content impact as shown in table 15 and Fig. 5, in 0d and the 30d time
Point, water content be 65% test group AA content be all remarkably higher than 70% and 75% (P < 0.05).At 60d time point, respectively organize it
Between significant difference (P < 0.05), and each group AA content sequence is 65% 75% 70%.At 10d time point, between each group
Difference is not notable (P > 0.05).It is also seen that the AA content of each test group is all in downward trend after first raising from table, and under
Range of decrease degree relatively raises slow.
Fructus Cucurbitae moschatae and corn straw are mixed the impact (%) of storage AA content by table 15 different in moisture
Note: same column letter shoulder mark difference represents significant difference (P < 0.05).
Seed-use pumpkin and corn straw are mixed the impact of storage effect by different grain size
Employing single factor experiment designs, and seed-use pumpkin is divided into, homogenate state, a diameter of 0.2cm's block more than 5cm is thread
Three granularities, mix microbial with corn straw (a length of 2cm) with the ratio of 1:1 respectively, and moisture Control is 70%.Test totally 3
Individual process group, each process 4 repetition.EXPERIMENTAL DESIGN is shown in Table 16.By (a length of to varigrained seed-use pumpkin, corn straw
2cm) and the water that adds is according to the ratio mixing calculated, it is loaded on rapidly in feed fermentation breathing bag after mixing, and immediately with true
Empty package machine carries out application of vacuum and plastic packaging.20～25 DEG C disposed within of feed fermentation breathing bag keeps in Dark Place.Respectively 0d,
10d, 30d and 60d, 4 point in time sampling carry out subjective appreciation, measure pH, AN, LAB, LA and AA, and measure the gathered sample of 0d
NDF, ADF and CP content of product
Table 16 EXPERIMENTAL DESIGN
Test data uses SPSS19.0 software that data carry out one factor analysis of variance, and carries out multiple by Duncan method
Relatively, using P ＜ 0.05 as significance of difference criterion.Result represents with mean+SD.
The sensory evaluation that different grain size Fructus Cucurbitae moschatae and corn straw mix storage is as shown in table 17.As can be seen from Table 17, M1 and M2
Sensory evaluation scores the highest be 19 points, belong to one-level ensilage (excellent).And the sensory evaluation scores of M0 is 15 points, belong to seconds
Matter (fair).
Table 17 different grain size Fructus Cucurbitae moschatae and corn straw mix the subjective appreciation of storage
The impact that Fructus Cucurbitae moschatae and corn straw are mixed storage pH value by different grain size is as shown in table 18, at 0d and 10d time point, and each group
Between difference all notable (P<0.05), the most notable (P>0.05) in 30d time point, M1 and M0 and M2 difference, but between M0 and M2
Significant difference (P < 0.05).When 60d, each group difference is not notable (P > 0.05), and its pH value size order is M2 > M0 > M1.
As seen from Figure 6, during whole microbial, the pH value of each group is all in the trend being gradually reduced, at microbial later stage (after 30d),
Trend is the mildest.
Fructus Cucurbitae moschatae and corn straw are mixed the impact of storage pH value by table 18 different grain size
Note: same column letter shoulder mark difference represents significant difference (P < 0.05).
Different grain size Fructus Cucurbitae moschatae and corn straw are mixed storage LAB number impact as shown in table 19 and Fig. 7, in 0d and the 30d time
Point, the LAB number of M0 be all substantially less than M1 and M2 (P<0.05), and the equal difference of LAB number between M1 and M2 two groups notable (P>
0.05).At 60d time point, each group difference is notable (P<0.05), and its LAB number size order is M2>M1>M0.By table 19 and figure
7 it is also seen that along with the carrying out of microbial, the LAB number of each group all declines, in first rising, the trend finally tended towards stability afterwards.
Fructus Cucurbitae moschatae and corn straw are mixed the impact (cfu/ml) of storage LAB number by table 19 different grain size
Note: same column letter shoulder mark difference represents significant difference (P < 0.05).
Different grain size Fructus Cucurbitae moschatae and corn straw are mixed storage LA content impact as shown in table 20 and Fig. 8, in 0d and the 10d time
Point, equal significant difference (P < 0.05) between each group.At 30d time point, M1 Yu M0 and M2 significant difference (P < 0.05), and M0, M2 two
Between person, difference is not notable (P > 0.05).At 60d time point, each group difference is not notable (P > 0.05), and its LA content size
Order is followed successively by M1 > M2 > M0.By Fig. 8 it is also seen that in the whole microbial phase, the LA content of M0 and M2 is all in gradually rising trend.
Fructus Cucurbitae moschatae and corn straw are mixed the impact (%) of storage LA content by table 20 different grain size
Note: same column letter shoulder mark difference represents significant difference (P < 0.05).
Different grain size Fructus Cucurbitae moschatae and corn straw are mixed storage CP and NDF, ADF content impact as shown in table 21, between each group
Albumen, NDF, ADF content difference be not the most notable (P > 0.05).The sequence of protein content and NDF content is M0 > M2 > M1,
The sequence of ADF content is M1 > M2 > M0.
Fructus Cucurbitae moschatae and corn straw are mixed storage CP and NDF, the impact of ADF content by table 21 different grain size
Note: same column letter shoulder mark difference represents significant difference (P < 0.05).
Fructus Cucurbitae moschatae and corn straw are mixed the impact of storage AN content by different grain size
As shown in table 22, at 0d time point, M0 Yu M1, the AN significant difference (P < 0.05) of M2, each group of 10d, 30d, 60d
Between difference notable (P > 0.05).As seen from Figure 9, each group AN is gradually reduced subsequently along with the carrying out of microbial first rises,
60d time point, the AN content sequence of each group is M1 > M0 > M2.
Fructus Cucurbitae moschatae and corn straw are mixed the impact (%) of storage AN content by table 22 different grain size
Fructus Cucurbitae moschatae and corn straw are mixed the impact of storage AA content as shown in table 23 and Figure 10 by different grain size.
Fructus Cucurbitae moschatae and corn straw are mixed the impact (%) of storage AA content by table 23 different grain size
From table 23 and Figure 10, at 0d and 10d time point, equal significant difference (P < 0.05) between each group, at 30d and 60d
Time point, equal significant difference (P<0.05) between M0 and M2 group, in microbial latter stage, the AA content sequence of each group is M0>M1>
M2。
Claims (9)
1. a seed-use pumpkin and corn straw fermented feed, it is characterised in that described fermented feed is by seed-use pumpkin silk and straw
Stalk powder is by for (4～9): the mass ratio of 6 is made.
A kind of seed-use pumpkin and corn straw fermented feed, it is characterised in that described fermented feed
It is made up by the mass ratio for 1:1 of seed-use pumpkin silk and powder of straw.
A kind of seed-use pumpkin the most according to claim 1 or claim 2 and corn straw fermented feed, it is characterised in that described seed south
A diameter of (0.20～0.40) cm of melon silk.
A kind of seed-use pumpkin the most according to claim 1 or claim 2 and corn straw fermented feed, it is characterised in that described corn stalk
A length of (2～3) cm of stalk powder.
A kind of seed-use pumpkin the most as claimed in claim 1 and the preparation method of corn straw fermented feed, it is characterised in that the party
Method is carried out in the steps below: seed-use pumpkin melon meat is processed into thread acquisition seed-use pumpkin silk, and corn straw smashing obtains
Powder of straw, add water after then seed-use pumpkin silk and powder of straw being mixed mixing, is loaded on rapidly and is provided with the fermentation of Non-return air valve and exhales
Sealing after in suction bag, store 45～60d as indoor lucifuge, described Non-return air valve is by the gas discharging in fermentation breathing bag.
A kind of seed-use pumpkin and the preparation method of corn straw fermented feed, it is characterised in that add
The moisture of water management solid content is 65%～75%.
A kind of seed-use pumpkin and the preparation method of corn straw fermented feed, it is characterised in that add
The moisture of water management solid content is 70%.
A kind of seed-use pumpkin and the preparation method of corn straw fermented feed, it is characterised in that room
Interior temperature controls lucifuge at 20～25 DEG C and stores.
A kind of seed-use pumpkin and the preparation method of corn straw fermented feed, it is characterised in that institute
State fermentation breathing bag be long 20cm, wide 35cm, the feed fermentation breathing bag of diameter 15cm.
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| CN104814265A (en) * | 2015-05-13 | 2015-08-05 | 安徽禾源柴达建材科技有限公司 | Preparation method of novel highland barley feed |
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| CN205305963U (en) * | 2015-12-27 | 2016-06-15 | 黑龙江省兽医科学研究所 | Straw fermenting installation |
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