CN105985912A - Alternaria alternata and application thereof - Google Patents
Alternaria alternata and application thereof Download PDFInfo
- Publication number
- CN105985912A CN105985912A CN201510061555.1A CN201510061555A CN105985912A CN 105985912 A CN105985912 A CN 105985912A CN 201510061555 A CN201510061555 A CN 201510061555A CN 105985912 A CN105985912 A CN 105985912A
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- CN
- China
- Prior art keywords
- tobacco leaf
- microbial inoculum
- application
- alternaria alternata
- tobacco
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- Granted
Links
- 241000223602 Alternaria alternata Species 0.000 title claims abstract description 52
- 241000208125 Nicotiana Species 0.000 claims abstract description 121
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 121
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims abstract description 26
- 150000001875 compounds Chemical class 0.000 claims abstract description 22
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 claims abstract description 13
- 229960002715 nicotine Drugs 0.000 claims abstract description 13
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims description 94
- 239000002068 microbial inoculum Substances 0.000 claims description 57
- 230000008569 process Effects 0.000 claims description 25
- IGFHQQFPSIBGKE-UHFFFAOYSA-N 4-nonylphenol Chemical compound CCCCCCCCCC1=CC=C(O)C=C1 IGFHQQFPSIBGKE-UHFFFAOYSA-N 0.000 claims description 24
- -1 1-(2-furyl) Ethyl Chemical group 0.000 claims description 23
- 241000894006 Bacteria Species 0.000 claims description 23
- FDPIMTJIUBPUKL-UHFFFAOYSA-N pentan-3-one Chemical compound CCC(=O)CC FDPIMTJIUBPUKL-UHFFFAOYSA-N 0.000 claims description 21
- MOQGCGNUWBPGTQ-UHFFFAOYSA-N 2,6,6-trimethyl-1-cyclohexene-1-carboxaldehyde Chemical compound CC1=C(C=O)C(C)(C)CCC1 MOQGCGNUWBPGTQ-UHFFFAOYSA-N 0.000 claims description 16
- 239000003205 fragrance Substances 0.000 claims description 16
- LWWOWQZGAGVVEU-UHFFFAOYSA-N 2,3,6-trimethylnaphthalene-1,4-dione Chemical compound CC1=CC=C2C(=O)C(C)=C(C)C(=O)C2=C1 LWWOWQZGAGVVEU-UHFFFAOYSA-N 0.000 claims description 14
- FLIACVVOZYBSBS-UHFFFAOYSA-N Methyl palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC FLIACVVOZYBSBS-UHFFFAOYSA-N 0.000 claims description 14
- AMQJEAYHLZJPGS-UHFFFAOYSA-N N-Pentanol Chemical compound CCCCCO AMQJEAYHLZJPGS-UHFFFAOYSA-N 0.000 claims description 14
- KSMVZQYAVGTKIV-UHFFFAOYSA-N decanal Chemical compound CCCCCCCCCC=O KSMVZQYAVGTKIV-UHFFFAOYSA-N 0.000 claims description 14
- XIRNKXNNONJFQO-UHFFFAOYSA-N ethyl hexadecanoate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC XIRNKXNNONJFQO-UHFFFAOYSA-N 0.000 claims description 14
- JARKCYVAAOWBJS-UHFFFAOYSA-N hexanal Chemical compound CCCCCC=O JARKCYVAAOWBJS-UHFFFAOYSA-N 0.000 claims description 14
- PQDRXUSSKFWCFA-UHFFFAOYSA-N solanone Natural products CC(=O)CCC(C(C)C)C=CC(C)=C PQDRXUSSKFWCFA-UHFFFAOYSA-N 0.000 claims description 14
- HUMNYLRZRPPJDN-UHFFFAOYSA-N benzaldehyde Chemical compound O=CC1=CC=CC=C1 HUMNYLRZRPPJDN-UHFFFAOYSA-N 0.000 claims description 12
- DOIRQSBPFJWKBE-UHFFFAOYSA-N dibutyl phthalate Chemical compound CCCCOC(=O)C1=CC=CC=C1C(=O)OCCCC DOIRQSBPFJWKBE-UHFFFAOYSA-N 0.000 claims description 12
- 150000001491 aromatic compounds Chemical class 0.000 claims description 11
- MGWAVDBGNNKXQV-UHFFFAOYSA-N diisobutyl phthalate Chemical compound CC(C)COC(=O)C1=CC=CC=C1C(=O)OCC(C)C MGWAVDBGNNKXQV-UHFFFAOYSA-N 0.000 claims description 10
- 239000000725 suspension Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 244000005700 microbiome Species 0.000 claims description 9
- 150000002148 esters Chemical class 0.000 claims description 8
- HZYHMHHBBBSGHB-UHFFFAOYSA-N (2E,6E)-2,6-Nonadienal Natural products CCC=CCCC=CC=O HZYHMHHBBBSGHB-UHFFFAOYSA-N 0.000 claims description 7
- HZYHMHHBBBSGHB-DYWGDJMRSA-N (2e,6e)-nona-2,6-dienal Chemical compound CC\C=C\CC\C=C\C=O HZYHMHHBBBSGHB-DYWGDJMRSA-N 0.000 claims description 7
- NMRPBPVERJPACX-UHFFFAOYSA-N (3S)-octan-3-ol Natural products CCCCCC(O)CC NMRPBPVERJPACX-UHFFFAOYSA-N 0.000 claims description 7
- 239000001674 (E)-1-(2,6,6-trimethyl-1-cyclohexenyl)but-2-en-1-one Substances 0.000 claims description 7
- WOFPPJOZXUTRAU-UHFFFAOYSA-N 2-Ethyl-1-hexanol Natural products CCCCC(O)CCC WOFPPJOZXUTRAU-UHFFFAOYSA-N 0.000 claims description 7
- YIWUKEYIRIRTPP-UHFFFAOYSA-N 2-ethylhexan-1-ol Chemical compound CCCCC(CC)CO YIWUKEYIRIRTPP-UHFFFAOYSA-N 0.000 claims description 7
- AZVSIHIBYRHSLB-UHFFFAOYSA-N 3-furaldehyde Chemical compound O=CC=1C=COC=1 AZVSIHIBYRHSLB-UHFFFAOYSA-N 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
- 229940067592 ethyl palmitate Drugs 0.000 claims description 7
- HNZUNIKWNYHEJJ-FMIVXFBMSA-N geranyl acetone Chemical compound CC(C)=CCC\C(C)=C\CCC(C)=O HNZUNIKWNYHEJJ-FMIVXFBMSA-N 0.000 claims description 7
- HNZUNIKWNYHEJJ-UHFFFAOYSA-N geranyl acetone Natural products CC(C)=CCCC(C)=CCCC(C)=O HNZUNIKWNYHEJJ-UHFFFAOYSA-N 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 7
- GYHFUZHODSMOHU-UHFFFAOYSA-N nonanal Chemical compound CCCCCCCCC=O GYHFUZHODSMOHU-UHFFFAOYSA-N 0.000 claims description 7
- PQDRXUSSKFWCFA-CFNZNRNTSA-N solanone Chemical compound CC(=O)CC[C@@H](C(C)C)\C=C\C(C)=C PQDRXUSSKFWCFA-CFNZNRNTSA-N 0.000 claims description 7
- BKOOMYPCSUNDGP-UHFFFAOYSA-N trimethyl-ethylene Natural products CC=C(C)C BKOOMYPCSUNDGP-UHFFFAOYSA-N 0.000 claims description 7
- WRMNZCZEMHIOCP-UHFFFAOYSA-N 2-phenylethanol Chemical compound OCCC1=CC=CC=C1 WRMNZCZEMHIOCP-UHFFFAOYSA-N 0.000 claims description 6
- QKSLBKHGKROAFN-UHFFFAOYSA-N 3,4-dihydropyrrole Chemical compound [CH]1CCC=N1 QKSLBKHGKROAFN-UHFFFAOYSA-N 0.000 claims description 6
- ISAVYTVYFVQUDY-UHFFFAOYSA-N 4-tert-Octylphenol Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(O)C=C1 ISAVYTVYFVQUDY-UHFFFAOYSA-N 0.000 claims description 6
- 150000001299 aldehydes Chemical class 0.000 claims description 6
- QNGNSVIICDLXHT-UHFFFAOYSA-N para-ethylbenzaldehyde Natural products CCC1=CC=C(C=O)C=C1 QNGNSVIICDLXHT-UHFFFAOYSA-N 0.000 claims description 6
- WSFSSNUMVMOOMR-UHFFFAOYSA-N formaldehyde Substances O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 5
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Natural products C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 230000032683 aging Effects 0.000 abstract description 11
- 239000004615 ingredient Substances 0.000 abstract description 3
- 150000002989 phenols Chemical class 0.000 abstract description 3
- 238000009629 microbiological culture Methods 0.000 abstract description 2
- 238000000855 fermentation Methods 0.000 description 12
- 230000004151 fermentation Effects 0.000 description 12
- 235000019504 cigarettes Nutrition 0.000 description 8
- 239000000203 mixture Substances 0.000 description 7
- 238000000926 separation method Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- MVOSYKNQRRHGKX-UHFFFAOYSA-N 11-Undecanolactone Chemical compound O=C1CCCCCCCCCCO1 MVOSYKNQRRHGKX-UHFFFAOYSA-N 0.000 description 3
- 241000223600 Alternaria Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
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- 239000002609 medium Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241000186221 Cellulosimicrobium cellulans Species 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 150000001555 benzenes Chemical class 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
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- DIOQZVSQGTUSAI-UHFFFAOYSA-N decane Chemical compound CCCCCCCCCC DIOQZVSQGTUSAI-UHFFFAOYSA-N 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- SNRUBQQJIBEYMU-UHFFFAOYSA-N dodecane Chemical compound CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 2
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- 239000003960 organic solvent Substances 0.000 description 2
- XNGIFLGASWRNHJ-UHFFFAOYSA-N phthalic acid Chemical compound OC(=O)C1=CC=CC=C1C(O)=O XNGIFLGASWRNHJ-UHFFFAOYSA-N 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000004904 shortening Methods 0.000 description 2
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- 239000005418 vegetable material Substances 0.000 description 2
- CKDOLMXYCOTPEK-UHFFFAOYSA-N 3,4-dihydro-2h-pyrrolo[1,2-a]pyrazin-1-one Chemical compound O=C1NCCN2C=CC=C12 CKDOLMXYCOTPEK-UHFFFAOYSA-N 0.000 description 1
- ACZGCWSMSTYWDQ-UHFFFAOYSA-N 3h-1-benzofuran-2-one Chemical compound C1=CC=C2OC(=O)CC2=C1 ACZGCWSMSTYWDQ-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- WVDDGKGOMKODPV-UHFFFAOYSA-N Benzyl alcohol Chemical group OCC1=CC=CC=C1 WVDDGKGOMKODPV-UHFFFAOYSA-N 0.000 description 1
- POAXRNROGRXJKW-UHFFFAOYSA-N C(CCC)OC(=O)CCCC.C1=CC=CC=C1 Chemical group C(CCC)OC(=O)CCCC.C1=CC=CC=C1 POAXRNROGRXJKW-UHFFFAOYSA-N 0.000 description 1
- 241000120652 Cellulomonas sp. Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000191938 Micrococcus luteus Species 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
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- 229920002472 Starch Polymers 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
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- 239000003963 antioxidant agent Substances 0.000 description 1
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- RHDGNLCLDBVESU-UHFFFAOYSA-N but-3-en-4-olide Chemical compound O=C1CC=CO1 RHDGNLCLDBVESU-UHFFFAOYSA-N 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
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- MGNZXYYWBUKAII-UHFFFAOYSA-N cyclohexa-1,3-diene Chemical compound C1CC=CC=C1 MGNZXYYWBUKAII-UHFFFAOYSA-N 0.000 description 1
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- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
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- LHGVFZTZFXWLCP-UHFFFAOYSA-N guaiacol Chemical compound COC1=CC=CC=C1O LHGVFZTZFXWLCP-UHFFFAOYSA-N 0.000 description 1
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- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
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Landscapes
- Manufacture Of Tobacco Products (AREA)
Abstract
The invention discloses alternaria alternata and an application thereof. The alternaria alternata HaoJ003 provided by the invention is preserved in China General Microbiological Culture Collection Center, with registration number of CGMCC No. 9852. Twenty eight tobacco related aroma components are detected from tobacco leaves which are processed by the alternaria alternata HaoJ003 CGMCC No. 9852 strain, wherein fourteen aroma compounds are not detected out from a control group, and the contents of other fourteen aroma components are improved in comparison with the control group, a nicotine content is reduced by 10.26% in comparison with the control group and the contents of harmful ingredients including one benzene, four phenols and the like are significantly declined in comparison with the control group; with the application of the alternaria alternata, tobacco aroma is enhanced, and meanwhile the contents of the harmful ingredients in the tobacco leaves are reduced; and the alternaria alternata can shorten a natural aging time and improve tobacco quality.
Description
Technical field
The present invention relates to a strain alternaric bacteria and application thereof in microbial technology field.
Background technology
The harmful substances such as the nicotine in tobacco leaf, nitrous Ammonia have had a strong impact on quality of tobacco, particularly upper tobacco leaf.On
In portion's tobacco leaf, dry circularity is high, and blade is thick, and weight per leaf is big, and the content of ethers extract is high, and fragrance is more sufficient,
But the content of total reducing sugar and reducing sugar is relatively low, the content of nicotine and protein is higher, in tobacco leaf various chemical compositions it
Between ratio inharmonious, miscellaneous gas and excitant are heavier, restriction tobacco leaf in intrinsic fragrance appear, have a strong impact on upper tobacco leaf
Practicality, causes huge economic loss.Additionally, this type of tobacco natural alcoholization speed is slow, storage period is longer,
Place take greatly, funds tied up are many thus cause production cost to increase, and are susceptible to insect pest in long term storage
With go mouldy, therefore how to process and apply this type of tobacco leaf to become a pendulum difficult problem urgently to be resolved hurrily in face of cigarette enterprise.
Microorganism species is to acceleration tobacco mellowing, raising quality of tobacco, increase cigarette flavor and reduces harmful matter in cigarette
Aspect has considerable effect.Developed country takes the lead in having carried out microorganism and reduces tobacco leaf harmful substance, increases tobacco
The research of fragrance, domestic many research teams did a large amount of similar research equally.V.L.Geiss(Control and
use of microbes in tobacco product manufacturing[J].Recent Advance in Tobacco
Science, 1989,15:182-209.) report micrococcus luteus and cellulomonas cartae (Cellulomonas sp.) can drop
Nitrate in low fermenting tobacco leaf, and cellulomonas cartae can also reduce the content of nicotine in tobacco leaf thus improve tobacco leaf
Quality.Zhu great Heng et al. (Zhu great Heng, Han Jinfeng drive wind etc. in week. utilize aroma-producing microbe fermenting and producing tobacco aromatics technology
And application [J]. tobacco science and technology, 1997,1:30-31.) from tobacco leaf, separate and purified a strain aroma former, utilize it
Tunning promotes tabacco fragrance as spices (TMF), and result cigarette quality is obviously improved, smoke alcohol and and full,
And cigarette foreign gas and excitant can be mitigated.Han Jinfeng et al. (Han Jinfeng, Zhu great Heng, Liu Weiqun. flue-cured tobacco during ageing fermentation
Phylloplane organisms activity and application study [J]. Chinese Tobacco Science, 1997,4:13-14.) be separated to from tobacco leaf several
Strain dominant bacteria, be hybridly prepared into biologic product (TFA) be used for tobacco fermentation, result show TFA can accelerating fermentation of leaf tobacco by use,
Improve tobacco fermentation quality, there is the mouldy effect of suppression tobacco leaf simultaneously.Zhao Mingqin et al. (Zhao Mingqin, Qi Weicheng, Qiu Li
Friend. tobacco fermentation increases the impact on flue-cured tobacco fermented quality for the preparation. Henan Agricultural Sciences, 1998,12:13-14.) utilize advantage
The amylase of flavouring bacterium and high bioactivity, protease are configured to tobacco fermentation increasing matter agent and are applied in tobacco fermentation, knot
Fruit discovery fermentation increases matter agent and has the internal organic material decomposition of promotion tobacco leaf and conversion, makes the compositions such as sugar nitrogen base in tobacco leaf
Content and ratio tend to balance, and tobacco aroma quality is improved, and perfume quantity increases, and intrinsic miscellaneous gas and excitant mitigate, and
Accelerating fermentation of leaf tobacco by use process, shortens fermentation period.But, can be used in micro-life of the quick flavouring of flue-cured tobacco and ageing at present
Reporting or fewer of thing bacterial strain, there is also flavouring DeGrain, fermentation time is long simultaneously, and technology application is multiple
Miscellaneous, microbial strains is difficult to the problems such as cultivation, far from the actual demand meeting cigarette industry development, therefore seeks one
Kind can significantly improve quality of tobacco, and accelerated ageing can be realized, it is simple to large-scale production and popularization and application, to the mankind and
Environment is all harmless, processes tobacco leaf safe and stable, does not cause the microbial strains going mouldy to be those skilled in the art's institute's phases
Hope.
Content of the invention
The technical problem to be solved is how to increase tabacco fragrance, reduces the Harmful ingredient content in tobacco leaf,
Shorten nature digestion time, improve quality of tobacco.
For solving above-mentioned technical problem, present invention firstly provides a strain alternaric bacteria.
Alternaric bacteria provided by the present invention is rod method (Alternaria alternata) HaoJ003, and it is in China
The numbered CGMCC No.9852 that registers on the books at Microbiological Culture Collection administration committee common micro-organisms center.This bacterium
Plant China Committee for Culture Collection of Microorganisms's common micro-organisms center that has been preserved in (letter on October 28th, 2014
Claim CGMCC).
Rod method (Alternaria alternata) the HaoJ003CGMCC No.9852 of the present invention cultivates at PDA
On base, colony growth is rapid, and the initial stage is canescence, after gradually present grey black concentric colyliform bacterium colony, bacterium colony carpet shape,
Smooth.Mycelia has separation, diameter 3-6 μm；Conidiophore tip flexion is irregular, brown, on it raw brown
Conidium, catenation；Spore rod capitate, has 1-3 mediastinum, 3-7 tabula, sometimes bends, spore
The conidium of subchain end is less, oval, has 1 separation.
Described rod method (Alternaria alternata) HaoJ003CGMCC No.9852 is producing conidium
And/or the application in mycelium falls within protection scope of the present invention.
For solving above-mentioned technical problem, present invention also offers a kind of microbial inoculum.
Microbial inoculum provided by the present invention, its active component is described rod method (Alternaria alternata)
HaoJ003CGMCC No.9852。
In above-mentioned microbial inoculum, HaoJ003CGMCC No.9852 can for described rod method (Alternaria alternata)
For conidium and/or mycelium.
Described microbial inoculum has following 1)-5) at least one application:
1) application in tabacco fragrance is increased；
2) application causing in aroma compounds is increased in tobacco leaf；Described cause aroma compounds be in following 14 kinds of compounds extremely
Few one: 2-ethyl-1-hexanol, 1-amylalcohol, very little visit alcohol, 1-(1H-pyrroles's-2-base) ethyl ketone, 1-(2-furyl)
Ethyl ketone, 3,4-dihydropyrrole simultaneously [1,2-a] pyrazine-1 (2H)-one, solanone, 3-furfural, capraldehyde, hexanal, 14
Aldehyde, diisobutyl phthalate, O-phthalic ester di-isooctyl and ethyl palmitate；
3) application in the content of aroma component in tobacco leaf is improved；Described aroma component is in following 14 kinds of aroma components
At least one: benzyl carbinol, 3,7-dimethyl-1,6-octadiene-3-alcohol, 2,3,6-trimethyl-1,4-naphthalenedione, 2,3-
Dihydrobenzofuranes ketone, 3-hydroxyl-BETA-damascone, 3-hydroxyl solanone, geranyl acetone, 2,6,6-front three
Base-1-cyclohexene-1-formaldehyde (cyclocitral), 2,6-nonadienal, 3-methyl-2-butene aldehyde, benzaldehyde, aldehyde C-9,
Dibutyl phthalate and methyl palmitate；
4) application in nicotine content in tobacco leaf is reduced；
5) application in the content of aromatic compound in tobacco leaf is reduced；Described aromatic compound is following 5 kinds of fragrance
At least one in compounds of group: ethylo benzene, 4-(1,1,3,3-tetramethyl butyl) phenol, 4-nonyl phenol, 2-first
Epoxide-4-Vinyl phenol and nonyl phenol.
Described microbial inoculum can also include carrier.Described carrier can be solid carrier or liquid-carrier.Described solid carrier can
For mineral material, vegetable material or macromolecular compound；Described mineral material can be clay, talcum, kaolin, illiteracy
At least one in de-stone, white carbon, zeolite, silica and diatomite；Described vegetable material can for corn flour, bean powder and
At least one in starch；Described macromolecular compound can be polyvinyl alcohol and/or polyglycols.Described liquid-carrier can be
Organic solvent, vegetable oil, mineral oil or water；Described organic solvent can be decane and/or dodecane.In described microbial inoculum,
Described active component can with the living cells, the zymotic fluid of living cells, the filtrate of cell culture or the cell that are cultured with
Presented in the mixture of filtrate.The formulation of described microbial inoculum can be multiple formulations, as liquor, emulsion, suspending agent,
Pulvis, granule, wettable powder or water dispersible granules.
As required, described microbial inoculum also can add surfactant (such as polysorbas20, Tween 80 etc.), adhesive,
Stabilizer (such as antioxidant), pH adjusting agent etc..
Described rod method (Alternaria alternata) HaoJ003CGMCC No.9852 or with described rod method
(Alternaria alternata) HaoJ003CGMCC No.9852 is following the 1 of the microbial inoculum of active component)-5)
In any one application fall within protection scope of the present invention:
1) application in tabacco fragrance is increased；
2) application causing in aroma compounds is increased in tobacco leaf；Described cause aroma compounds be in following 14 kinds of compounds extremely
Few one: 2-ethyl-1-hexanol, 1-amylalcohol, very little visit alcohol, 1-(1H-pyrroles's-2-base) ethyl ketone, 1-(2-furyl)
Ethyl ketone, 3,4-dihydropyrrole simultaneously [1,2-a] pyrazine-1 (2H)-one, solanone, 3-furfural, capraldehyde, hexanal, 14
Aldehyde, diisobutyl phthalate, O-phthalic ester di-isooctyl and ethyl palmitate；
3) application in the content of aroma component in tobacco leaf is improved；Described aroma component is in following 14 kinds of aroma components
At least one: benzyl carbinol, 3,7-dimethyl-1,6-octadiene-3-alcohol, 2,3,6-trimethyl-1,4-naphthalenedione, 2,3-
Dihydrobenzofuranes ketone, 3-hydroxyl-BETA-damascone, 3-hydroxyl solanone, geranyl acetone, 2,6,6-front three
Base-1-cyclohexene-1-formaldehyde (cyclocitral), 2,6-nonadienal, 3-methyl-2-butene aldehyde, benzaldehyde, aldehyde C-9,
Dibutyl phthalate and methyl palmitate；
4) application in nicotine content in tobacco leaf is reduced；
5) application in the content of aromatic compound in tobacco leaf is reduced；Described aromatic compound is following 5 kinds of fragrance
At least one in compounds of group: ethylo benzene, 4-(1,1,3,3-tetramethyl butyl) phenol, 4-nonyl phenol, 2-first
Epoxide-4-Vinyl phenol and nonyl phenol.
In order to solve above-mentioned technical problem, present invention also offers the described rod method (Alternaria of cultivation
Alternata) method of HaoJ003CGMCC No.9852.
Described rod method (Alternaria alternata) the HaoJ003CGMCC No.9852 of cultivation provided by the present invention
Method, including by described rod method (Alternaria alternata) HaoJ003CGMCC No.9852 with
The step cultivated in the culture medium cultivating alternaric bacteria.
For solving above-mentioned technical problem, present invention also offers the method preparing described microbial inoculum.
The method preparing above-mentioned microbial inoculum provided by the present invention, including by described rod method (Alternaria
Alternata) HaoJ003CGMCC No.9852 is as active component, obtains the step of described microbial inoculum.
The described method preparing microbial inoculum, it may include cultivate described rod method (Alternaria in liquid medium within
Alternata) step of HaoJ003CGMCC No.9852；Described fluid nutrient medium concretely PD culture medium.
For solving above-mentioned technical problem, present invention also offers the method improving quality of tobacco.
The method improving quality of tobacco provided by the present invention, hangs including use the described microbial inoculum of sterilized water dilution to obtain microbial inoculum
Supernatant liquid, sprays described microbial inoculum suspension in pending tobacco leaf surface, is aged, and obtains microbial inoculum and processes tobacco leaf.
In said method, described microbial inoculum processes tobacco leaf and processes compared with tobacco leaf with sterilized water, has following 1)-5) in
At least one characteristic:
1) tabacco fragrance is increased；
2) increase in tobacco leaf and cause aroma compounds；Described cause aroma compounds is at least one in following 14 kinds of compounds:
2-ethyl-1-hexanol, 1-amylalcohol, very little visit alcohol, 1-(1H-pyrroles's-2-base) ethyl ketone, 1-(2-furyl) ethyl ketone, 3,4-
Dihydropyrrole simultaneously [1,2-a] pyrazine-1 (2H)-one, solanone, 3-furfural, capraldehyde, hexanal, undecalactone, adjacent benzene two
Diisobutyl formate, O-phthalic ester di-isooctyl and ethyl palmitate；
3) content of aroma component in tobacco leaf is improved；Described aroma component is at least in following 14 kinds of aroma components
Kind: benzyl carbinol, 3,7-dimethyl-1,6-octadiene-3-alcohol, 2,3,6-trimethyl-1,4-naphthalenedione, 2,3-dihydrobenzene
And furanone, 3-hydroxyl-BETA-damascone, 3-hydroxyl solanone, geranyl acetone, 2,6,6-trimethyl-1-
Cyclohexene-1-formaldehyde (cyclocitral), 2,6-nonadienal, 3-methyl-2-butene aldehyde, benzaldehyde, aldehyde C-9, adjacent benzene
Dibutyl carboxylic acid and methyl palmitate；
4) nicotine content in tobacco leaf is reduced；
5) content of aromatic compound in tobacco leaf is reduced；Described aromatic compound is following 5 kinds of aromatic compounds
In at least one: ethylo benzene, 4-(1,1,3,3-tetramethyl butyl) phenol, 4-nonyl phenol, 2-methoxyl group-4-
Vinylphenol and nonyl phenol.
Wherein, described sterilized water processes the preparation method of tobacco leaf except by the described bacterium in the above-mentioned method improving quality of tobacco
Outside the sterilized water of the quality such as agent suspension replaces with, other are all identical.
In the above-mentioned method improving quality of tobacco, rod method (Alternaria described in described microbial inoculum suspension
Alternata) content of HaoJ003CGMCC No.9852 can be 1 × 106cfu/mL-8×106Cfu/mL, specifically
Can be 1 × 106cfu/mL。
In the above-mentioned method improving quality of tobacco, described ageing can be 20 ± 2% at relative air humidity, and temperature is 60
The environment of ± 1 DEG C is carried out.
In the above-mentioned method improving quality of tobacco, described ageing can be carried out 90 days.
The microbial inoculum obtaining according to the above-mentioned method improving quality of tobacco processes tobacco leaf and falls within protection scope of the present invention.
Rod method (Alternaria alternata) the HaoJ003CGMCC No.9852 of the present invention, to the mankind and ring
Border is all harmless, uses safety；Process tobacco leaf safe and stable, do not cause and go mouldy.Use the rod method of the present invention
(Alternaria alternata) HaoJ003CGMCC No.9852 microbial inoculum processes stock top, bottom cigarette
Leaf, is substantially shorter the fermentation digestion time of tobacco leaf, reduces a large amount of tobacco leaf warehousing pressure.Use the rod method of the present invention
The tobacco leaf that (Alternaria alternata) HaoJ003CGMCC No.9852 microbial inoculum is processed, detects 28 kinds of cigarettes
Leaf causes fragrant Related Component, and wherein 14 kinds of cause aroma compounds do not detect in control group, remaining 14 kinds of aroma component content
All improving than control group, nicotine content is than control group reduction the 10.26%th, the harmful components such as a kind of benzene class and 4 kinds of phenols
Content is all remarkably decreased than control group.Rod method (Alternaria alternata) HaoJ003CGMCC of the present invention
No.9852 microbial inoculum not only adds tabacco fragrance but also reduce the content of harmful components in tobacco leaf, when shortening nature ageing
Between, improve quality of tobacco.
Preservation explanation
Strain name: rod method
Latin name: Alternaria alternata
Strain number: HaoJ003
Preservation mechanism: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation mechanism is called for short: CGMCC
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Preservation date: on October 28th, 2014
Register on the books numbering in preservation center: CGMCC No.9852
Brief description
Fig. 1 is the bacterium colony of rod method (Alternaria alternata) HaoJ003CGMCC No.9852.
Fig. 2 is the thalli morphology of rod method (Alternaria alternata) HaoJ003CGMCC No.9852.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is further described in detail, the embodiment being given only for
Illustrate the present invention, rather than in order to limit the scope of the present invention.
Experimental technique in following embodiment, if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all commercially obtain.
The separation of embodiment the 1st, rod method (Alternaria alternata) HaoJ003CGMCC No.9852
And identify
1st, the separation of rod method (Alternaria alternata) HaoJ003CGMCC No.9852
Gather warehouse storage (ageing) tobacco leaf and field period tobacco leaf sample, under 40 × stereoscope, directly observe tobacco leaf
Surface microorganism, separates single cell culture in an aseptic environment, cultivates one week at 25 DEG C, rich according to colonial morphology, mycelia
The rich representational single bacterium colony of the feature picking such as degree and sporulation quantity purifies, and as test strains, is separated to 1000 altogether
Strain microbial strains.1000 strain test strains of isolated are configured to spray bacterium solution carry out spraying place to tobacco leaf respectively
Reason, be 20% in relative humidity, temperature be 60 DEG C under the conditions of ageing 90 days after to process after tobacco leaf in chemical composition
The content of (soluble sugar, nicotine, total nitrogen and carotenoid etc.) and change thereof are measured, and smoke panel test in conjunction with expert
The good and bad technical indicator of quality of tobacco, screening obtains rod method (Alternaria alternata) HaoJ003CGMCC
No.9852。
2nd, the qualification of rod method (Alternaria alternata) HaoJ003CGMCC No.9852
(1) Morphological Identification
Rod method (Alternaria alternata) HaoJ003CGMCC No.9852 bacterium colony on PDA culture medium
Rapidly, the initial stage is canescence in growth, after gradually present grey black concentric colyliform bacterium colony, bacterium colony carpet shape, smooth.Bacterium
Silk has separation, diameter 3-6 μm；Conidiophore tip flexion is irregular, brown, on it the mitogenetic spore of raw brown
Son, catenation；Spore rod capitate, has 1-3 mediastinum, 3-7 tabula, sometimes bends, spore chain end
Conidium less, oval, have 1 separation.
(2) Molecular Identification
DNA is extracted after rod method (Alternaria alternata) HaoJ003CGMCC No.9852 is cultivated,
Identify that this bacterial strain is rod method (Alternaria alternata) by fungi rDNA-ITS sequence alignment analysis.
The rDNA-ITS sequence of rod method (Alternaria alternata) HaoJ003CGMCC No.9852 is sequence
RDNA sequence shown in SEQ ID No.1 in list.
Rod method (Alternaria alternata) HaoJ003CGMCC No.9852 is in October, 2014
Within 28th, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (be called for short CGMCC), its
The numbered CGMCC No.9852 that registers on the books at China Committee for Culture Collection of Microorganisms's common micro-organisms center.
Embodiment the 2nd, rod method (Alternaria alternata) HaoJ003CGMCC No.9852 microbial inoculum is used for
Improve quality of tobacco
1st, the preparation of rod method (Alternaria alternata) HaoJ003CGMCC No.9852 microbial inoculum
Rod method (Alternaria alternata) HaoJ003CGMCC No.9852 is used PD Liquid Culture
Base is cultivated, and the preparation method of PD culture medium is as follows: potato is cleaned peeling, weighs 200g, be cut into small pieces,
Add water and boil 20-30min, by filtered through gauze, add 20g glucose, stir, after slightly cooling down, supply moisture again
To 1000mL, regulate pH value to 5, be distributed into three shape bottles, 121 DEG C of autoclaving about 20min, store after cooling
Deposit standby.
By rod method (Alternaria alternata) HaoJ003CGMCC No.9852 with PDA culture medium (
PD culture medium adds the solid medium that agar obtains) in 25 DEG C of activation culture, beating with diameter 8mm after activation
Hole device makes bacterium cake in colony edge punching, moves into bacterium cake in the 500mL triangular flask containing 200mL PD nutrient solution,
Each triangular flask moves into 4 ferfas cakes, is placed in 25 DEG C, 10d cultivated by the shaking table of 120rpm/min.Cultivation terminates
After utilize sonicator to crush mycelium and spore, ultrasonic power is 800w, and each ultrasonic time is 3s,
Interval 3s, ultrasonic 45 times altogether, is prepared as rod method (Alternaria alternata) HaoJ003CGMCC No.9852
Microbial inoculum.Rod method (Alternaria alternata) HaoJ003CGMCC No.9852 microbial inoculum is sub-packed in 500mL
In Brown Glass Brown glass bottles and jars only, 4 DEG C save backup.
2nd, tobacco leaf is improved with rod method (Alternaria alternata) HaoJ003CGMCC No.9852 microbial inoculum
Quality
Gather after the tobacco leaf of Yun yan85 is ripe, take the top that water content is 11-13% and be randomly divided into two groups, real
Test and be divided into microbial inoculum process group and control group.The tobacco leaf of microbial inoculum process group is handled as follows: use sterilized water dilution step
Rod method (Alternaria alternata) the HaoJ003CGMCC No.9852 microbial inoculum of rapid 1 obtains microbial inoculum and hangs
Supernatant liquid, rod method (Alternaria alternata) the HaoJ003CGMCC No.9852 in this microbial inoculum suspension
Content be 1 × 106cfu/mL.Spray this microbial inoculum suspension in pending tobacco leaf surface, the spray of this microbial inoculum suspension
The amount of executing is the 1% of pending quality of tobacco, is 20% at relative air humidity, and temperature is ageing 90 in the environment of 60 DEG C
My god, obtain microbial inoculum and process tobacco leaf.
Except by addition to the sterilized water of the quality such as microbial inoculum suspension replaces with, other are all identical in the tobacco leaf processing method of control group,
Obtain control treatment tobacco leaf.
The microbial inoculum quality such as taking respectively processes tobacco leaf and control treatment tobacco leaf, according to document (Xiao Xiezhong, Wu Guoying, king
Precious China etc. tobacco chemistry [M]. Beijing: Chinese agriculture publishing house, 1997:50,65,165 194.) survey of described method
Determine the nicotine content in tobacco leaf；The microbial inoculum quality such as taking respectively processes tobacco leaf and the automatic gas phase look of control treatment tobacco leaf picking
Spectrum-mass spectrometry instrument (GC/MS), uses Wiley and NIST spectrum storehouse to carry out collection of illustrative plates retrieval and analyze, determines bacterium respectively
Agent processes species and the title of the Related Component containing in tobacco leaf and control treatment tobacco leaf；Use standard items according to standard
Curve method (external standard method) carries out quantitative analysis and measures the phase containing in microbial inoculum process tobacco leaf and control treatment tobacco leaf respectively
Close the content of composition.Each sample is repeated three times averages, and measuring the results are shown in Table 1.
Table the 1st, rod method microbial inoculum processes tobacco leaf Related Component assay result
Note: "×" represents without this material in comparison, "-" represents that in process tobacco leaf, this content of material reduces, and "--" represents should
Numerical value cannot calculate.
From table 1 it follows that use rod method (Alternaria alternata) HaoJ003CGMCC No.9852
Detecting 34 kinds of Related Components in tobacco leaf after microbial inoculum process altogether, wherein, 28 kinds is that tobacco leaf causes fragrant Related Component, bag
Include 5 kinds of alcohols and (2-ethyl-1-hexanol, 1-amylalcohol, very little visit alcohol, benzyl carbinol, 3,7-dimethyl-1,6-octadiene-3-
Alcohol), 9 kinds of ketones (1-(1H-pyrroles's-2-base) ethyl ketone, 1-(2-furyl) ethyl ketone, 3,4-dihydropyrrole simultaneously [1,2-a]
Pyrazine-1 (2H)-one, solanone, 2,3,6-trimethyl-1,4-naphthalenedione, 2,3-Dihydrobenzofuranes ketone, 3-hydroxyl-BETA-
Damascone, 3-hydroxyl solanone, geranyl acetone), 9 kinds of aldehydes (3-furfural, capraldehyde, hexanal, undecalactone,
2,6,6-trimethyl-1-cyclohexene-1-formaldehyde (cyclocitral), 2,6-nonadienal, 3-methyl-2-butene aldehyde, benzene first
Aldehyde, aldehyde C-9) and 5 kinds of esters (diisobutyl phthalate, O-phthalic ester di-isooctyl, ethyl palmitate,
Dibutyl phthalate, methyl palmitate).In 28 kinds of aroma components, 14 kinds are had to cause aroma compounds (2-ethyl
-1-hexanol, 1-amylalcohol, very little visit alcohol, 1-(1H-pyrroles's-2-base) ethyl ketone, 1-(2-furyl) ethyl ketone, 3,4-dihydro
Pyrrolo-[1,2-a] pyrazine-1 (2H)-one, solanone, 3-furfural, capraldehyde, hexanal, undecalactone, phthalic acid two
Isobutyl ester, O-phthalic ester di-isooctyl and ethyl palmitate) do not detect in control group；Remaining 14 kinds of aroma component
(benzyl carbinol, 3,7-dimethyl-1,6-octadiene-3-alcohol, 2,3,6-trimethyl-1,4-naphthalenedione, 2,3-dihydrobenzo
Furanone, 3-hydroxyl-BETA-damascone, 3-hydroxyl solanone, geranyl acetone, 2,6,6-trimethyl-1-hexamethylene
Alkene-1-formaldehyde (cyclocitral), 2,6-nonadienal, 3-methyl-2-butene aldehyde, benzaldehyde, aldehyde C-9, O-phthalic
Dibutyl phthalate and methyl palmitate) content all than control group improve；Nicotine content comparison in microbial inoculum process group tobacco leaf
According to group reduction by 10.26%, a kind of benzene class (ethylo benzene) in microbial inoculum process group tobacco leaf and 4 kinds of phenols (4-(1,1,3,3-
Tetramethyl butyl) phenol, 4-nonyl phenol, 2-methoxyl-4-vinyl phenol and nonyl phenol) etc. harmful components
Content be all remarkably decreased than control group.Rod method (Alternaria alternata) HaoJ003CGMCC of the present invention
No.9852 microbial inoculum not only adds tabacco fragrance but also reduce the content of harmful components in tobacco leaf, when shortening nature ageing
Between, improve quality of tobacco.
Claims (10)
1. rod method (Alternaria alternata), it is characterised in that: described rod method (Alternaria
Alternata) bacterial strain number is HaoJ003, and it is in China Committee for Culture Collection of Microorganisms's common micro-organisms
The numbered CGMCC No.9852 that registers on the books of the heart.
2. the rod method described in claim 1 (Alternaria alternata) is producing conidium and/or bacterium
Application in filament.
3. a microbial inoculum, its active component is the rod method (Alternaria alternata) described in claim 1.
4. microbial inoculum according to claim 3, it is characterised in that: described microbial inoculum has following 1)-5) and at least
A kind of application:
1) application in tabacco fragrance is increased；
2) application causing in aroma compounds is increased in tobacco leaf；Described cause aroma compounds be in following 14 kinds of compounds extremely
Few one: 2-ethyl-1-hexanol, 1-amylalcohol, very little visit alcohol, 1-(1H-pyrroles's-2-base) ethyl ketone, 1-(2-furyl)
Ethyl ketone, 3,4-dihydropyrrole simultaneously [1,2-a] pyrazine-1 (2H)-one, solanone, 3-furfural, capraldehyde, hexanal, 14
Aldehyde, diisobutyl phthalate, O-phthalic ester di-isooctyl and ethyl palmitate；
3) application in the content of aroma component in tobacco leaf is improved；Described aroma component is in following 14 kinds of aroma components
At least one: benzyl carbinol, 3,7-dimethyl-1,6-octadiene-3-alcohol, 2,3,6-trimethyl-1,4-naphthalenedione, 2,3-
Dihydrobenzofuranes ketone, 3-hydroxyl-BETA-damascone, 3-hydroxyl solanone, geranyl acetone, 2,6,6-front three
Base-1-cyclohexene-1-formaldehyde (cyclocitral), 2,6-nonadienal, 3-methyl-2-butene aldehyde, benzaldehyde, aldehyde C-9,
Dibutyl phthalate and methyl palmitate；
4) application in nicotine content in tobacco leaf is reduced；
5) application in the content of aromatic compound in tobacco leaf is reduced；Described aromatic compound is following 5 kinds of fragrance
At least one in compounds of group: ethylo benzene, 4-(1,1,3,3-tetramethyl butyl) phenol, 4-nonyl phenol, 2-first
Epoxide-4-Vinyl phenol and nonyl phenol.
5. rod method described in claim 1 (Alternaria alternata), or, described in claim 3 or 4
Following the 1 of microbial inoculum)-5) in any one application:
1) application in tabacco fragrance is increased；
2) application causing in aroma compounds is increased in tobacco leaf；Described cause aroma compounds be in following 14 kinds of compounds extremely
Few one: 2-ethyl-1-hexanol, 1-amylalcohol, very little visit alcohol, 1-(1H-pyrroles's-2-base) ethyl ketone, 1-(2-furyl)
Ethyl ketone, 3,4-dihydropyrrole simultaneously [1,2-a] pyrazine-1 (2H)-one, solanone, 3-furfural, capraldehyde, hexanal, 14
Aldehyde, diisobutyl phthalate, O-phthalic ester di-isooctyl and ethyl palmitate；
3) application in the content of aroma component in tobacco leaf is improved；Described aroma component is in following 14 kinds of aroma components
At least one: benzyl carbinol, 3,7-dimethyl-1,6-octadiene-3-alcohol, 2,3,6-trimethyl-1,4-naphthalenedione, 2,3-
Dihydrobenzofuranes ketone, 3-hydroxyl-BETA-damascone, 3-hydroxyl solanone, geranyl acetone, 2,6,6-front three
Base-1-cyclohexene-1-formaldehyde (cyclocitral), 2,6-nonadienal, 3-methyl-2-butene aldehyde, benzaldehyde, aldehyde C-9,
Dibutyl phthalate and methyl palmitate；
4) application in nicotine content in tobacco leaf is reduced；
5) application in the content of aromatic compound in tobacco leaf is reduced；Described aromatic compound is following 5 kinds of fragrance
At least one in compounds of group: ethylo benzene, 4-(1,1,3,3-tetramethyl butyl) phenol, 4-nonyl phenol, 2-first
Epoxide-4-Vinyl phenol and nonyl phenol.
6. the method cultivating rod method (Alternaria alternata) described in claim 1, including by described chain
The step that lattice spore (Alternaria alternata) is cultivated in the culture medium for cultivating alternaric bacteria.
7. the method preparing microbial inoculum described in claim 3 or 4, including by described rod method (Alternaria
Alternata) as active component, the step of described microbial inoculum is obtained.
8. the method improving quality of tobacco, including use microbial inoculum described in sterilized water dilution claim 3 or 4 to obtain microbial inoculum
Suspension, sprays described microbial inoculum suspension in pending tobacco leaf surface, is aged, and obtains microbial inoculum and processes tobacco leaf.
9. method according to claim 8, it is characterised in that: in described microbial inoculum suspension described in claim 1
The content of rod method (Alternaria alternata) is 1 × 106cfu/mL-8×106cfu/mL。
10. the microbial inoculum obtaining according to method described in claim 8 or 9 processes tobacco leaf.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10405571B2 (en) | 2015-06-26 | 2019-09-10 | Altria Client Services Llc | Compositions and methods for producing tobacco plants and products having altered alkaloid levels |
| CN113243391A (en) * | 2021-05-21 | 2021-08-13 | 山东农业大学 | Sterilization composition containing Taishan mycin |
| CN114907990A (en) * | 2022-05-02 | 2022-08-16 | 中国海洋大学 | Alternaria alternata and application thereof in removing protein in waste diatomite |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102851217A (en) * | 2012-08-30 | 2013-01-02 | 云南省烟草农业科学研究院 | Culture medium for Alternaria alternata and preparation method thereof |
| CN104207324A (en) * | 2013-05-30 | 2014-12-17 | 贵州中烟工业有限责任公司 | Tobacco leaf processing method |
-
2015
- 2015-02-05 CN CN201510061555.1A patent/CN105985912B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102851217A (en) * | 2012-08-30 | 2013-01-02 | 云南省烟草农业科学研究院 | Culture medium for Alternaria alternata and preparation method thereof |
| CN104207324A (en) * | 2013-05-30 | 2014-12-17 | 贵州中烟工业有限责任公司 | Tobacco leaf processing method |
Non-Patent Citations (4)
| Title |
|---|
| ELENA T. YAKIMOVA ET AL: "Alternaria alternata AT Toxin Induces Programmed Cell Death in Tobacco", 《J PHYTOPATHOL》 * |
| S. SLAVOV ET AL: "Toxin Production of Alternaria Alternata Tobacco", 《BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT》 * |
| 徐慧 等: "云南烟草叶片内生及叶际细菌、真菌多样性研究", 《云南农业大学学报》 * |
| 杨欣: "链格孢霉毒素研究进展", 《国外医学 卫生学分册》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10405571B2 (en) | 2015-06-26 | 2019-09-10 | Altria Client Services Llc | Compositions and methods for producing tobacco plants and products having altered alkaloid levels |
| CN113243391A (en) * | 2021-05-21 | 2021-08-13 | 山东农业大学 | Sterilization composition containing Taishan mycin |
| CN114907990A (en) * | 2022-05-02 | 2022-08-16 | 中国海洋大学 | Alternaria alternata and application thereof in removing protein in waste diatomite |
| CN114907990B (en) * | 2022-05-02 | 2023-07-14 | 中国海洋大学 | Alternaria alternata and application thereof in removal of protein in waste diatomite |
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