CN105982979B - Tender leaf extract of prinsepia utilis royle and preparation method and application thereof - Google Patents
Tender leaf extract of prinsepia utilis royle and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a tender leaf extract of prinsepia utilis royle and a preparation method and application thereof. The preparation method comprises any one of the following two methods: the method I comprises the following steps: immersing the crushed tender leaves of the prinsepia utilis royle in an ethanol solution, then extracting for 0.2-1.5 hours, carrying out solid-liquid separation to obtain filtrate, and carrying out reduced pressure rotary steaming on the filtrate until the filtrate is dried to obtain crude extracts of the tender leaves of the prinsepia utilis royle; method II: (1) preparing the crude extract of the tender leaves of the prinsepia utilis royle of the method I; (2) dissolving the crude extract with deionized water, loading onto macroporous resin packed column, sequentially eluting with solvent I, solvent II and solvent III, respectively collecting and removing solvents to obtain elution part A, elution part B and elution part C. The preparation method is simple and convenient, easy to operate, high in yield and suitable for popularization and application; the extract has various components which are mutually synergistic, and has better effects of anti-aging, anti-oxidation and anti-aging activity.
Description
Technical Field
The invention relates to a tender leaf extract of prinsepia utilis royle and a preparation method and application thereof.
Background
Prinsepia utilis (Prinsepia utilis), Prinsepia utilis, Rosaceae, Prinsepia genus plant, and other name such as Cannabis fruit, milk hammer, egg cake, plum blossom thorn, pistachio, anacis, Mongolia semen. The prinsepia utilis royle seeds are rich in oil, the oil yield is about 30 percent generally, the oil is dark brown yellow, clear and transparent, and is white like lard oil after solidification; the oil can be used for edible use, soap making, and lighting. The tender tip can be eaten as vegetable. The prinsepia utilis royle grows in hillside, stream side or bush with elevation of 1000-2650m and is mainly distributed in provinces such as Yunnan, Guizhou, Sichuan, Taiwan, Tibet and the like. Qingci Jian recorded in Dian nan Ben Cao, the day: cold in property and bitter in taste. All sores and carbuncles with pus discharging and pus disappearing immediately are attacked; taking after drinking wine for dispersing tuberculosis and chewing; the root, stem, leaf and fruit of the Chinese medicinal composition can be used as medicines, and the Chinese medicinal composition has been applied to the national minority of the nationalities of Naxi, Bai nationality, Yi nationality, Mozuo and the like for thousands of years. According to the record of modern data, the stem and leaf of the prinsepia utilis royle are mainly used for treating carbuncle, deep-rooted carbuncle, toxic sore, toothache due to wind-fire evil, gunshot wound, fracture and snake bite; the root is mainly used for treating deficiency cough, chronic cough, food retention and rheumatoid arthritis; the fruit is mainly used for treating nebula hyperdacryosis, dyspepsia and the like. Medical workers at home and abroad separate and identify a flavonoid component, namely, levo-epicatechin, a lignan component, namely, echinitol, and fatty acid components, namely, palmitic acid, stearic acid, arachidic acid, oleic acid, linoleic acid, linolenic acid and the like from the plant; the pharmacological research improves cell metabolism, enhances organism immunity, and has the functions of regulating human body blood lipid, promoting blood vessel softening, and delaying aging.
The research of the prinsepia utilis royle is mainly focused on the research of the prinsepia utilis royle oil, and the research on the tender leaves of the prinsepia utilis royle is less. The tender leaves of the prinsepia utilis royle are rich in resources, and the development of the tender leaves of the prinsepia utilis royle is necessary for further research and effective and reasonable development and utilization of the prinsepia utilis royle. At present, the civic uses the tender leaf tips of the prinsepia utilis royle to decoct and can repeatedly rinse the mouth with the decoction to treat damp-heat erosion of the oral cavity and fever caused by faucitis of children; parching herba Prinsepiae with Mel, and decocting with water for treating external hemorrhoid hemorrhage; the external application of the tender leaf tips of the prinsepia utilis royle is used for treating sores, carbuncles and pyogenic infections; the root of Prinsepia utilis is decocted with water, mixed with rice flour, decocted with water and raked for treating food retention. At present, in the prior art, the research on the extract of the prinsepia utilis royle leaves is only used for screening the anti-tumor effect of the prinsepia utilis royle leaf extract, and the application and report of the prinsepia utilis royle leaf extract in the field of cosmetics, particularly anti-aging, anti-oxidation activity and anti-aging are not found, and in the prior art, in the specific extraction process of the prinsepia utilis royle leaf extract, the preparation method of the extracted active ingredients is not easy to reproduce, the yield is low, and the method is not suitable for popularization and application.
Disclosure of Invention
The invention aims to overcome the defects that in the specific extraction process of the prinsepia utilis royle leaf extract in the prior art, the method for extracting active ingredients is not easy to reproduce, the yield is low, the prinsepia utilis royle leaf extract is not suitable for popularization and application, and the antitumor effect is only researched, and innovatively provides the prinsepia utilis royle leaf extract and the preparation method and application thereof. The preparation method of the tender leaf extract of prinsepia utilis royle is simple and convenient, easy to operate, high in yield and suitable for popularization and application; the prinsepia utilis royle tender leaf extract has multiple components, and the mutual synergistic effect of the components plays a role in improving the anti-aging effect of the extract, and has better anti-aging, anti-oxidation and anti-aging activity.
The invention provides a preparation method of a tender leaf extract of prinsepia utilis royle, which comprises any one of the following two methods:
the method I comprises the following steps:
immersing the crushed tender leaves of the prinsepia utilis royle in an ethanol solution with the volume concentration of 50-80%, then extracting for 0.2-1.5 hours, carrying out solid-liquid separation to obtain a filtrate, and carrying out reduced pressure rotary steaming on the filtrate until the filtrate is dried to obtain a crude extract of the tender leaves of the prinsepia utilis royle; wherein the mass-volume ratio of the tender leaves of the prinsepia utilis royle to the ethanol solution is (1: 8) - (1: 15) g/mL;
method II:
(1) immersing the crushed tender leaves of the prinsepia utilis royle in an ethanol solution with the volume concentration of 50-80%, then extracting for 0.2-1.5 hours, carrying out solid-liquid separation to obtain a filtrate, and carrying out reduced pressure rotary steaming on the filtrate until the filtrate is dried to obtain a crude extract of the tender leaves of the prinsepia utilis royle; wherein the mass-volume ratio of the tender leaves of the prinsepia utilis royle to the ethanol solution is (1: 8) - (1: 15) g/mL;
(2) dissolving the crude extract of the tender leaves of the prinsepia utilis royle by using deionized water, loading the extract into a macroporous resin packed column, eluting by using a solvent I, a solvent II and a solvent III in sequence, respectively collecting eluent, and correspondingly obtaining an elution part A, an elution part B and an elution part C after removing the solvent; wherein the solvent I is an ethanol solution with the volume concentration of 25-35%, the solvent II is an ethanol solution with the volume concentration of 45-55%, and the solvent III is an ethanol solution with the volume concentration of 65-75%.
In method I and method II, step (1), the immersion is conventional in the art and is generally carried out at room temperature.
In method I and method II, step (1), the extraction is a routine procedure in the art, preferably ultrasonic extraction. The temperature of the ultrasonic extraction is preferably at room temperature. The room temperature is conventional in the art and is typically 15-25 ℃. The time for the ultrasonic extraction is preferably 0.5 hour. The number of times of the ultrasonic extraction is preferably 2.
In process I and process II, step (1), the reduced pressure rotary evaporation is conventional in the art.
In the method I and method II, step (1), the ethanol solution preferably has a concentration of 65 to 75% by volume, more preferably 70% by volume.
In the step (1) of the methods I and II, the mass-to-volume ratio of the young leaf of prinsepia utilis royle to the ethanol solution is preferably (1: 9) - (1: 11), more preferably 1: 10.
in the step (2) of the method II, the macroporous resin packed column is a macroporous resin packed column which is conventional in the field, and is generally an AB-8 resin packed column.
In the step (2) of the method II, the solvent I is preferably a 30 vol% ethanol solution. The solvent II is preferably ethanol solution with the volume concentration of 50%. The solvent III is preferably ethanol solution with the volume concentration of 70%.
Method II in step (2), the solvent removal method is conventional in the art, and preferably reduced pressure rotary evaporation.
The invention also provides the tender leaf extract of the prinsepia utilis royle prepared by the preparation method.
In the invention, the prinsepia utilis royle tender leaf extract is one or more of the prinsepia utilis royle tender leaf crude extract, the elution part A, the elution part B and the elution part C, and is preferably the elution part C.
The invention also provides application of the prinsepia utilis royle tender leaf extract as an anti-aging active ingredient or an anti-oxidation active ingredient in preparation of a skin external preparation.
Preferably, the tender leaf extract of prinsepia utilis royle is used as a free radical scavenger, an elastase inhibitor, a fibroblast proliferation promoter or a collagen type I generation promoter when preparing a skin external preparation.
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
The reagents and starting materials used in the present invention are commercially available.
The positive progress effects of the invention are as follows:
1. the tender leaf extract of the prinsepia utilis has multiple component types, and the mutual synergistic effect of various components plays a role in improving the anti-aging effect of the extract, and has better anti-aging, anti-oxidation and anti-aging effects.
2. The preparation method of the tender leaf extract of prinsepia utilis royle is simple and convenient, easy to operate, high in yield and suitable for popularization and application.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.
Example 1
20g of the dry powder of the tender leaves of the prinsepia utilis royle, immersing the tender leaves of the prinsepia utilis royle in 200mL of ethanol solution with the volume concentration of 50% at room temperature, namely the mass-to-volume ratio (material-to-liquid ratio) of the dry powder of the prinsepia utilis royle to the ethanol solution is 1:10, ultrasonic extracting for 2 times, each time 30min (500W), combining filtrates, decompressing and rotary steaming to dry to obtain 2.05g of the crude extract of the tender leaf of the prinsepia utilis royle, wherein the yield is 10.25%.
Example 2
20g of the prinsepia utilis royle dry powder, immersing the prinsepia utilis royle dry powder in 200mL of ethanol solution with the volume concentration of 60% at room temperature, namely, the mass-to-volume ratio (material-to-liquid ratio) of the prinsepia utilis royle dry powder to the ethanol solution is 1:10, ultrasonic extracting for 2 times, each time 30min (500W), combining filtrates, decompressing and rotary steaming to dry to obtain 2.12g of the crude extract of the tender leaf of the prinsepia utilis royle, wherein the yield is 10.60%.
Example 3
20g of the prinsepia utilis royle dry powder, immersing the prinsepia utilis royle dry powder in 200mL of ethanol solution with the volume concentration of 70% at room temperature, namely, the mass-to-volume ratio (material-to-liquid ratio) of the prinsepia utilis royle dry powder to the ethanol solution is 1:10, ultrasonic extracting for 2 times, each time 30min (500W), combining filtrates, decompressing and rotary steaming to dry to obtain 2.24g of the crude extract of the tender leaf of the prinsepia utilis royle, wherein the yield is 11.20%.
Example 4
20g of the prinsepia utilis royle dry powder, immersing the prinsepia utilis royle dry powder in 200mL of ethanol solution with the volume concentration of 80% at room temperature, namely, the mass-to-volume ratio (material-to-liquid ratio) of the prinsepia utilis royle dry powder to the ethanol solution is 1:10, ultrasonic extracting for 2 times, each time 30min (500W), combining filtrates, decompressing and rotary steaming to dry to obtain 2.28g of crude extract of tender leaves of prinsepia utilis royle, wherein the yield is 11.40%.
Example 5
20g of the prinsepia utilis royle dry powder, immersing the prinsepia utilis royle dry powder in 160mL of ethanol solution with the volume concentration of 70% at room temperature, namely, the mass-to-volume ratio (material-to-liquid ratio) of the prinsepia utilis royle dry powder to the ethanol solution is 1: 8, carrying out ultrasonic extraction for 2 times, each time for 30min (500W), combining filtrates, carrying out rotary steaming under reduced pressure until the filtrates are dried to obtain 2.02g of the crude extract of the tender leaves of the prinsepia utilis royle, wherein the yield is 10.10%.
Example 6
20g of the prinsepia utilis royle dry powder, immersing the prinsepia utilis royle dry powder in 240mL of ethanol solution with the volume concentration of 70% at room temperature, wherein the mass-to-volume ratio (material-to-liquid ratio) of the prinsepia utilis royle dry powder to the ethanol solution is 1:12, ultrasonic extracting for 2 times, each time 30min (500W), combining filtrates, and rotary steaming under reduced pressure to dry to obtain 2.27g of crude extract of tender leaf of Prinsepia utilis Royle, with yield of 11.35%.
Example 7
20g of the prinsepia utilis royle dry powder, and immersing the prinsepia utilis royle dry powder into 300mL of ethanol solution with the volume concentration of 70% at room temperature, namely the mass-volume ratio (material-liquid ratio) of the prinsepia utilis royle dry powder to the ethanol solution is 1: 15, ultrasonic extracting for 2 times, each time 30min (500W), combining filtrates, decompressing and rotary steaming to dry to obtain 2.29g of the crude extract of the tender leaf of the prinsepia utilis royle, wherein the yield is 11.45%.
Example 8
Immersing the dry powder of the prinsepia utilis royle 50g in 500mL of ethanol solution with the volume concentration of 70% at room temperature, ultrasonically extracting for 2 times, 30min (500W) each time, combining about 900mL of filtrate, and carrying out reduced pressure rotary steaming until the filtrate is dried to obtain 5.63g of the crude extract of the tender leaves of the prinsepia utilis royle, wherein the yield is 11.25%.
Dissolving the crude extract of the tender leaves of the prinsepia utilis royle by using deionized water, loading the extract into an AB-8 resin packed column, eluting by using 30% ethanol solution, 50% ethanol solution and 70% ethanol solution in sequence, collecting eluates (about 400mL each), removing the solvent, and respectively obtaining 1.85g of the elution part of the 30% ethanol solution of the tender leaves of the prinsepia utilis royle extract, wherein the yield is 30.85%, 1.05g of the elution part of the 50% ethanol solution is 18.65%, 0.78g of the elution part of the 70% ethanol solution is 13.85%.
Example 9
The extraction of the crude extract of young leaves of prinsepia utilis was the same as that of example 8.
Dissolving the crude extract of the tender leaves of the prinsepia utilis royle by using deionized water, loading the extract into an AB-8 resin packed column, eluting by using an ethanol solution with the volume concentration of 25%, an ethanol solution with the volume concentration of 55% and an ethanol solution with the volume concentration of 75% in sequence, respectively collecting eluates, and after removing a solvent, respectively obtaining an elution part of the 25% ethanol solution, an elution part of the 55% ethanol solution and an elution part of the 75% ethanol solution of the extract of the tender leaves of the prinsepia utilis royle.
Example 10
The extraction of the crude extract of young leaves of prinsepia utilis was the same as that of example 8.
Dissolving the crude extract of the tender leaves of the prinsepia utilis royle by using deionized water, loading the extract into an AB-8 resin packed column, eluting by using 35% ethanol solution, 45% ethanol solution and 65% ethanol solution in sequence, collecting eluates respectively, and obtaining the elution part of the 35% ethanol solution, the elution part of the 45% ethanol solution and the elution part of the 65% ethanol solution of the prinsepia utilis royle extract after removing the solvent.
Effect example 1
1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH) is a stable nitrogen-centered organic radical. The DPPH method was proposed in 1958 and widely used to quantitatively determine the anti-aging ability of biological samples, classified substances and foods. The method is based on the characteristic that DPPH free radical has single electron, strong absorption is provided at 517nm, and alcoholic solution is purple. When the free radical scavenger exists, the free radical scavenger is absorbed and disappears gradually due to the pairing with a single electron of the free radical scavenger, and the fading degree and the number of the received electrons form a quantitative relation, so that a spectrophotometer can be used for carrying out rapid quantitative analysis to detect the free radical scavenging condition, and the antioxidant activity and the anti-aging capability of a sample are evaluated.
The determination of DPPH free radical scavenging ability comprises the following steps: the crude extracts of the tender leaves of the prinsepia utilis royle of the examples 1 to 7 are respectively prepared into water solutions by deionized water, 2mL of the water solutions are transferred into a 10mL test tube with a plug, and 2mL of ethanol solution (2 multiplied by 10) of LDPPH is added-4mol/L), mixing well, and keeping the temperature at room temperatureStanding, measuring absorbance A at wavelength of 517nm with spectrophotometer after 30min517(ii) a Simultaneously measuring the absorbance A of 2mL of extract solution mixed with 2mL of ethanol0Absorbance C after 2mL of water mixed with 2mL of DPPH ethanol solution and absorbance C after 2mL of water mixed with 2mL of ethanol solution0. The measurement is carried out in three times in parallel, the average value is taken, the free radical clearance rate is calculated according to the following formula, and the larger the clearance rate is, the stronger the antioxidant capacity of the extract is. Examples 1-7 the results of measuring the radical scavenging activity of the crude extract of young leaves of prinsepia utilis royle are shown in table 1.
Radical scavenging rate (%) - (1- (a)517-A0)/(C-C0)]×100％。
TABLE 1 measurement results of radical scavenging Activity of examples 1 to 7
As can be seen from Table 1, when the volume concentration of ethanol in the tender leaves of prinsepia utilis royle is 70% and the ratio of the ethanol to the material to the liquid is 1: 10-1: 12, the crude extract of the tender leaves of the prinsepia utilis royle, which is obtained by extraction, has excellent free radical scavenging capability.
Effect example 2
The extract of the prinsepia utilis royle prepared in example 8 was taken and tested for its ability to scavenge DPPH radicals. The determination steps are as follows: preparing the tender leaf extract of Prinsepia utilis Royle with deionized water to obtain water solution, transferring 2mL into 10mL test tube with plug, adding 2mL of alcohol solution of LDPPH (2 × 10)-4mol/L), mixing, standing at room temperature, measuring absorbance A at wavelength of 517nm with spectrophotometer after 30min517(ii) a Simultaneously measuring the absorbance A of 2mL of extract solution mixed with 2mL of ethanol0Absorbance C after 2mL of water mixed with 2mL of DPPH ethanol solution and absorbance C after 2mL of water mixed with 2mL of ethanol solution0. The measurement is carried out in three times in parallel, the average value is taken, the free radical clearance rate is calculated according to the following formula, and the larger the clearance rate is, the stronger the antioxidant capacity of the extract is. Example 8 the results of measuring the radical scavenging activity of the young leaf extract of prinsepia utilis are shown in table 2.
Radical scavenging rate (%) - (1- (a)517-A0)/(C-C0)]×100％。
TABLE 2 measurement results of radical scavenging activity of example 8
As can be seen from Table 2, the extract of Prinsepia utilis has a strong ability to scavenge free radicals, and the 70% ethanol-eluted part of Prinsepia utilis, which is obtained by separation with AB-8 resin, has the strongest activity to scavenge free radicals.
Effect example 3
The present effect example examined the anti-aging activity of the tender leaf extract of prinsepia utilis royle. The tender leaf extract of prinsepia utilis as prepared in example 8 was dissolved in deionized water to prepare an aqueous solution for assaying elastase inhibitory activity. The determination method is carried out according to a literature method (am.j.pharmacol.toxicola, 2009,4, 127-: 10uL of the sample solution and 130uL of a 0.1M Tris-Cl buffer solution (pH 8.0) containing 1.015mM of the reaction substrate Succ-Ala-Ala-Ala-p-nitroanilide were added to a 96-well plate, incubated at 25 ℃ for 5 minutes, 15uL of an elastase solution (0.5U/mL) was added, incubation at 25 ℃ was continued for 30 minutes, and then the absorbance A at a wavelength of 410nm was measured with a microplate reader410. The absorbance was also measured as a reference solution by replacing the sample aqueous solution with deionized water, and the results are shown in Table 3. The inhibition rate is calculated, and the higher the inhibition rate is, the better the activity is.
The calculation formula of the elastase activity inhibition rate is as follows: inhibition ratio (%) ═ a0-(A410-B))/A0X 100% where A0Is the absorbance of a reference solution, A410Is the absorbance of the sample solution, and B is the absorbance of the sample solution diluted 3 times.
TABLE 3 Elastase inhibition results of tender leaf extract of Prinsepia utilis Royle of EXAMPLE 8
As is clear from table 3, the extracts of young leaves of prinsepia utilis royle used in the present invention all had an elastase inhibitory effect, and among them, the extract of young leaves of prinsepia utilis royle, which had a 70% ethanol elution portion with the highest elastase inhibitory effect, was able to be incorporated into an external preparation and used as an anti-aging agent for preventing skin aging and maintaining a young and healthy skin state.
Effect example 4
The present examples examine the effect of the prinsepia utilis royle extract on the proliferation of fibroblasts and the generation of type I collagen. The tender leaf extract of prinsepia utilis as prepared in example 8 was prepared into a 0.02% strength solution with deionized water, and added to human fibroblast culture medium with deionized water containing no sample as a blank control. After culturing for 48 hours, after staining the cells by MTT method, measuring absorbance A550 at 550nm by a microplate reader, evaluating the proliferation effect on human fibroblasts, wherein the proliferation rate of the blank control is 100%, and the proliferation rate higher than 120% is the proliferation effect on the cells. Taking cell supernatant sample, and determining collagen production with type I collagen determination kit, wherein the increase rate of blank control is 100%, and the increase rate is more than 100% for promoting collagen production.
TABLE 4 example 8 Effect of tender leaf extract of Prinsepia utilis Royle on fibroblast proliferation and type I collagen production
As can be seen from table 4, the extracts of prinsepia utilis royle used in the present invention all had an effect of promoting fibroblast proliferation and collagen production, and among them, the 70% ethanol portion of prinsepia utilis royle had the strongest effect of promoting fibroblast proliferation and collagen production.
Claims (10)
1. A preparation method of an anti-aging or anti-oxidation tender leaf extract of prinsepia utilis royle is characterized by comprising any one of the following two methods:
the method I comprises the following steps:
immersing the crushed tender leaves of the prinsepia utilis royle in an ethanol solution with the volume concentration of 65-80%, then extracting for 0.2-1.5 hours, carrying out solid-liquid separation to obtain a filtrate, and carrying out reduced pressure rotary steaming on the filtrate until the filtrate is dried to obtain a crude extract of the tender leaves of the prinsepia utilis royle; wherein the mass-volume ratio of the tender leaves of the prinsepia utilis royle to the ethanol solution is 1: 9-1: 15 g/mL;
method II:
(1) immersing the crushed tender leaves of the prinsepia utilis royle in an ethanol solution with the volume concentration of 65-80%, then extracting for 0.2-1.5 hours, carrying out solid-liquid separation to obtain a filtrate, and carrying out reduced pressure rotary steaming on the filtrate until the filtrate is dried to obtain a crude extract of the tender leaves of the prinsepia utilis royle; wherein the mass-volume ratio of the tender leaves of the prinsepia utilis royle to the ethanol solution is 1: 9-1: 15 g/mL;
(2) dissolving the crude extract of the tender leaves of the prinsepia utilis royle by using deionized water, loading the extract into a macroporous resin packed column, eluting by using a solvent I, a solvent II and a solvent III in sequence, respectively collecting eluent, and correspondingly obtaining an elution part B corresponding to the solvent II and an elution part C corresponding to the solvent III after removing the solvent; wherein the solvent I is an ethanol solution with the volume concentration of 25-35%, the solvent II is an ethanol solution with the volume concentration of 45-55%, and the solvent III is an ethanol solution with the volume concentration of 65-75%;
in the step (1) of the method I and the method II, the extraction is ultrasonic extraction and is carried out at room temperature.
2. The method of claim 1, wherein the time of the ultrasonic extraction is 0.5 hours;
and/or the number of times of ultrasonic extraction is 2.
3. The method of claim 1, wherein the ethanol solution has a concentration of 65 to 75% by volume in step (1) of methods I and II.
4. The method according to claim 1, wherein in the step (1) of the methods I and II, the mass-to-volume ratio of the young leaves of the prinsepia utilis royle to the ethanol solution is 1: 9-1: 11.
5. the process according to claim 1, wherein in step (2) of the process II, the macroporous resin packed column is an AB-8 resin packed column;
and/or, in the step (2) of the method II, the method for removing the solvent is reduced pressure rotary evaporation.
6. The method according to claim 1, wherein in the step (2), the solvent II is a 50% ethanol solution, and the solvent III is a 70% ethanol solution.
7. An extract of young leaves of Prinsepia utilis Royle obtained by the method according to any one of claims 1 to 6.
8. The prinsepia utilis royle leaf extract as claimed in claim 7, wherein the prinsepia utilis royle leaf extract is one or more of the prinsepia utilis royle leaf crude extract, the elution part B and the elution part C.
9. Use of the tender leaf extract of prinsepia utilis royle according to claim 7 or 8 as an anti-aging active ingredient, or, an anti-oxidation active ingredient for the preparation of an external preparation for skin.
10. The use of the extract of young leaves of prinsepia utilis royle as a free radical scavenger, an elastase inhibitor, a fibroblast proliferation promoter, or a collagen type I production promoter in the preparation of an external preparation for skin as claimed in claim 9.
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