CN105974093A - Method for determining total nitration of soil in non-rhizosphere zone - Google Patents
Method for determining total nitration of soil in non-rhizosphere zone Download PDFInfo
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- CN105974093A CN105974093A CN201610563696.8A CN201610563696A CN105974093A CN 105974093 A CN105974093 A CN 105974093A CN 201610563696 A CN201610563696 A CN 201610563696A CN 105974093 A CN105974093 A CN 105974093A
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Abstract
The invention provides a method for determining total nitration of soil in a non-rhizosphere zone. In an ecological system, a 20m*20m sample land is selected; in the sample land, 10 small units are selected according to a wild quincunx-shaped sampling method; secondly, sampling points with plants are selected from the small units, and soil samples are taken in north, south, east, west, northeast, northwest, southeast and southwest eight directions from the horizontal plane to plants and are mixed; a soil body with a root system is carefully dug from each direction, and large-particle soil attached on the root system is slightly removed by a sterilized soil cutter; non-rhizosphere soil is added into a sterilized self-sealing bag by the sterilized soil cutter; the taken soil samples are put into a heat-preservation box with ice blocks to be stored as soon as possible and are transported back to a laboratory; and finally, a BaPS soil nitrogen circulating monitoring system is used for determining the total nitration speed of the soil of the non-rhizosphere zone. The method provided by the invention has important effects on further knowing that a whole soil microorganism zone system participates in global biochemical nitrogen circulation.
Description
Technical field
The present invention relates to biogeochemistry field, specifically, relate to a kind of total nitrification of bulk soil soil that measures
Method.
Background technology
Soil nitrification is the important step of biogeochemistry Nitrogen Cycling, under the effect of soil microorganism, and soil
Ammoniacal nitrogen in earth is oxidized to nitrous acid, and is oxidized to the process of nitrate further；Its Biochemical processes are produced
Raw various nitrogen oxides such as NO, N2O, is not only a main path of the loss of nitrogen, and atmosphere pollution.It addition, N2O is
A kind of greenhouse gases, after entering air, can make O3Protective layer is destroyed, and directly jeopardizes atmosphere and biosphere.Meanwhile, N2O
Can also produce secondary chemical reaction with the organic chemical composition in air and become the main source of haze, atmosphere pollution, to people
Class life brings harm.And point out, from 1970 to 2004 terms according to Intergovernmental Panel on Climate Change (IPCC) research
Between greenhouse gas emission add 70%.N2O is a kind of metastable greenhouse gases, and its global warming potential is CO2296
Times, between 1970 to 2004 years, its concentration 270ppm before the industrial revolution rises to the 319ppm of 2005.
Soil microorganism is as the Primary Actor (Fig. 1) of soil nitrification (Gross nitrification), non-
Rhizosphere district soil microbial activities has the characteristic of uniqueness, thus the assay method of bulk soil soil Gross nitrification is really
Fixed, carbon nitrogen balance, the N slowing down in air are being regulated for understanding bulk soil soil ecosystem2O isothermal chamber gas concentration
Rise and safeguard that Global climate change has irreplaceable effect.
The shallow soil being commonly considered as distance root surface l-5mm is rhizosphere soil, thus bulk soil soil refers to directly
Receive that a part of soil scope relatively weak of root system of plant impact.Affected by root system of plant vital movement, non-rhizosphere
District's root exudates is the most active on the impact of the material of soil ecosystem, energy exchange, thus bulk soil environment has
The physics of uniqueness, chemistry, biological characteristic, the soil denitrification process in this region there is also non-rhizosphere effect.Therefore, bulk soil
The mensuration of soil denitrification effect is to further appreciating that soil ecosystem especially bulk soil soil nitrogen biogeochemical
Learn cyclic process and there is important function.
In existing technology, only measure soil release N2O greenhouse gases study soil ecosystem at regulation carbon nitrogen
Balance, and the determination of the assay method of the total nitrifying process of bulk soil soil has not yet to see report.
Summary of the invention
It is an object of the invention to provide a kind of method measuring the total nitrification of bulk soil soil.
In order to realize the object of the invention, present invention firstly provides the general of bulk soil soil total nitrifying process assay method
Read, it is desirable to provide a kind of quantitative determination bulk soil soil Gross nitrification method.
A kind of method measuring bulk soil soil Gross nitrification of the present invention, first, chooses 20m in ecosystem
The sample ground of × 20m, in sample ground, by field quincunx sampling methods, chooses the junior unit of 10 1m × 1m；Secondly, at little list
Choosing the sample point with plant in unit, at horizontal range plant 20,80cm, soil sampling mixing, picks with sterilized soil cutter
Except the bulky grain soil being attached on root system, with sterilized soil cutter, non-rhizosphere soils is loaded sterilizing valve bag, will take
Obtain soil sample and be immediately placed in the couveuse preservation with ice cube, transport laboratory back；Finally, BaPS Soil Nitrogen cycle monitoring system is utilized
Measure the bulk soil total rate of nitrification of soil.
Aforesaid method, fetches Non-rhizosphere soil sample and is measured in 24h.
Aforesaid method, the time utilizing BaPS Soil Nitrogen cycle monitoring system to measure is 12h.
Aforesaid method, the earth that fetches earth at described horizontal range plant 20,80cm refers in east, south, west, north, the southeast, east
North, southwest, orientation, 8, northwest take pedotheque.
Aforesaid method, described valve bag a size of: wide 17cm × long 24cm.
Aforesaid method, described in take pedotheque volume and be: long 20cm × wide 20cm × deep 50cm is with root system
The soil body.
Described ecosystem includes forest, meadow or farmland.
Advantages of the present invention effect is as follows:
The total nitrifying process of soil is biogeochemistry nitrogen cycle and N2The main process of O isothermal chamber gas release, existing
Technology in, only measure whole soil and include root system-microorganism-the litter hybrid system release N2O isothermal chamber gas is studied
Soil ecosystem is regulating ecosystem carbon, nitrogen balance, and the determination for bulk soil soil Gross nitrification is current
The method that there is no measures intuitively.Thus, the present invention, by rejecting non-bulk soil soil, extracts the bulk soil non-rhizosphere of soil test
District's soil Gross nitrification, the bulk soil enlivened for the whole material of understanding, energy exchange participates in geobiochemistry nitrogen
Circulation has important effect, can be that research soil microorganism is being tackled by Intergovernmental Panel on Climate Change (IPCC)
The impact of Global Emissions of Greenhouse Gas provides basic data to support；It addition, put down at carbon, nitrogen for understanding bulk soil biotic environment
The directive significance that the pivotal role played in weighing apparatus is important.
Accompanying drawing explanation
Fig. 1 soil microorganism participates in the schematic diagram of soil nitrification.
Fig. 2 is the schematic diagram of Semen Tritici aestivi bulk soil soil Gross nitrification.
Fig. 3 is the schematic diagram of Semen Maydis bulk soil soil Gross nitrification.
Fig. 4 is the quincunx point sampling method used in the embodiment of the present invention.
Fig. 5 is that 8 orientation used in the embodiment of the present invention fetch earth the method for sample.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.If not specializing, embodiment
In the conventional means that is well known to those skilled in the art of technological means used, raw materials used be commercial goods.
The air pressure process related in following example separates (BaPS) technology and utilizes BaPS Soil Nitrogen cycle monitoring system to enter
The mensuration of the total rate of nitrification of row soil, this system is purchased from UMS company of Germany.
Embodiment measures the method for bulk soil, farmland soil total nitre effect
First, Semen Tritici aestivi, milpa are chosen the sample ground of 20m × 20m, in sample ground, by field quincunx sampling methods (figure
3) junior unit of 10 1m × 1m, is chosen；Secondly, in junior unit, the sample point with plant is chosen, horizontal range plant
20, east, south, west, north at 80cm, the southeast, northeast, southwest, the orientation soil sampling mixing of 8, northwest, every orientation carefully takes length
20cm × wide 20cm × deep 50cm, with the soil body of root system, rejects gently with sterilized soil cutter and is attached on root system
Bulky grain soil, loads wide 17cm × long 24cm sterilizing valve bag with sterilized soil cutter by non-rhizosphere soils, will obtain
Soil sample is immediately placed in the couveuse preservation with ice cube, transports laboratory back；Advanced air pressure process is utilized to separate (BaPS) technology
Measure the bulk soil total rate of nitrification of soil.
Aforesaid method, fetches Non-rhizosphere soil sample and is measured in 24h.
Aforesaid method, the time utilizing BaPS Soil Nitrogen cycle monitoring system to measure is 12h.
Determination step is as follows:
1) before the assay, bulk soil soil sample put into sensing chamber；
2), when measuring, keep indoor temperature consistent with temperature during soil sampling, and measure 12 hours；
3), at the end of measuring, software BaPSSoftware Version 2.2.4 is utilized to calculate the total rate of nitrification of soil.
By calculating, Semen Tritici aestivi bulk soil soil Gross nitrification is respectively as follows: March: 18.33 ± 3.07 μ gN/kg
SDW h；April: 26.77 ± 3.38 μ gN/kg SDW h；May: 39.77 ± 4.18 μ gN/kg SDW h；June:
52.71±5.20 μgN/kg SDW h；July: 58.60 ± 8.18 μ gN/kg SDW h；August: 49.56 ± 7.04 μ
gN/kg SDW h；JIUYUE: 40.49 ± 4.07 μ gN/kg SDW h；October: 23.21 ± 2.18 μ gN/kg SDW h；11
Month: 13.69 ± 2.14 μ gN/kg SDW h；Wheatland annual bulk soil soil Gross nitrification is 35.91 ±
16.07 μgN/kg SDW h。
Milpa bulk soil earth Gross nitrification is respectively as follows: March: 6.96 ± 2.22 μ gN/kg SDW h；April:
13.96±2.53 μgN/kg SDW h；May: 32.86 ± 3.33 μ gN/kg SDW h；June: 41.59 ± 4.35 μ
gN/kg SDW h；July: 50.86 ± 7.33 μ gN/kg SDW h；August: 41.60 ± 6.19 μ gN/kg SDW h；JIUYUE
Part: 24.57 ± 3.22 μ gN/kg SDW h；October: 14.71 ± 1.33 μ gN/kg SDW h；November: 3.38 ± 1.29
μgN/kg SDW h；Milpa annual bulk soil soil Gross nitrification is 25.61 ± 16.95 μ gN/kg SDW h.
It is suitable for meadow or forest land, although, the most right
The present invention has made detailed description, but on the basis of the present invention, can make some modifications or improvements it, and this is to art technology
It is apparent from for personnel.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to
In the scope of protection of present invention.
Claims (7)
1. the method measuring bulk soil soil Gross nitrification, it is characterised in that first, chooses 20m in ecosystem
The sample ground of × 20m, in sample ground, by field quincunx sampling methods, chooses the junior unit of 10 1m × 1m；Secondly, at little list
Choosing the sample point with plant in unit, at horizontal range plant 20,80cm, soil sampling mixing, picks with sterilized soil cutter
Except the bulky grain soil being attached on root system, with sterilized soil cutter, non-rhizosphere soils is loaded sterilizing valve bag, will take
Obtain soil sample and be immediately placed in the couveuse preservation with ice cube, transport laboratory back；Finally, BaPS Soil Nitrogen cycle monitoring system is utilized
Measure the bulk soil total rate of nitrification of soil.
A kind of method measuring bulk soil soil Gross nitrification the most according to claim 1, it is characterised in that fetch
Non-rhizosphere soil sample is measured in 24 h.
A kind of method measuring bulk soil soil Gross nitrification the most according to claim 1, it is characterised in that utilize
The time that BaPS Soil Nitrogen cycle monitoring system measures is 12h.
A kind of method measuring bulk soil soil Gross nitrification the most according to claim 1, it is characterised in that described
The earth that fetches earth at horizontal range plant 20,80cm refers in east, south, west, north, the southeast, northeast, southwest, orientation, 8, northwest fetch earth earth
Sample.
A kind of method measuring bulk soil soil Gross nitrification the most according to claim 1, it is characterised in that described
Valve bag is a size of: wide 17cm × long 24cm.
A kind of method measuring bulk soil soil Gross nitrification the most according to claim 1, it is characterised in that described
Taking pedotheque volume is: long 20cm × wide 20cm × deep 50cm is with the soil body of root system.
A kind of method measuring bulk soil soil Gross nitrification the most according to claim 1, it is characterised in that described
Ecosystem include forest, meadow or farmland.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202903750U (en) * | 2012-12-04 | 2013-04-24 | 重庆大学 | Soil nitrification and denitrification in-situ measurement device |
| CN103383389A (en) * | 2013-06-26 | 2013-11-06 | 北京林业大学 | Airtight measuring chamber for BaPS soil nitrogen cycle monitoring system and application method thereof |
| CN103728433A (en) * | 2013-12-19 | 2014-04-16 | 北京林业大学 | Method for measuring contribution rate of plant root system to nitrification and denitrification of soil |
| CN103728434A (en) * | 2013-12-19 | 2014-04-16 | 北京林业大学 | Method for measuring contribution rate of forest litters to nitrification and denitrification of soil |
| RU2013129190A (en) * | 2013-06-25 | 2014-12-27 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Ижевская государственная сельскохозяйственная академия" | LABORATORY METHOD FOR DETERMINING NITRIFICATION SOIL ABILITY |
| DE102015013574A1 (en) * | 2014-10-16 | 2016-04-21 | Clemens Erbacher | PLATFORM FOR THE BACTERIAL NITRIFICATION OF WATER AND / OR FOR THE GROWING OF NON-ORGANIC PLANT CULTURES OVER WATERPROOF AND WET-WATER AREAS |
-
2016
- 2016-07-18 CN CN201610563696.8A patent/CN105974093A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202903750U (en) * | 2012-12-04 | 2013-04-24 | 重庆大学 | Soil nitrification and denitrification in-situ measurement device |
| RU2013129190A (en) * | 2013-06-25 | 2014-12-27 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Ижевская государственная сельскохозяйственная академия" | LABORATORY METHOD FOR DETERMINING NITRIFICATION SOIL ABILITY |
| CN103383389A (en) * | 2013-06-26 | 2013-11-06 | 北京林业大学 | Airtight measuring chamber for BaPS soil nitrogen cycle monitoring system and application method thereof |
| CN103728433A (en) * | 2013-12-19 | 2014-04-16 | 北京林业大学 | Method for measuring contribution rate of plant root system to nitrification and denitrification of soil |
| CN103728434A (en) * | 2013-12-19 | 2014-04-16 | 北京林业大学 | Method for measuring contribution rate of forest litters to nitrification and denitrification of soil |
| DE102015013574A1 (en) * | 2014-10-16 | 2016-04-21 | Clemens Erbacher | PLATFORM FOR THE BACTERIAL NITRIFICATION OF WATER AND / OR FOR THE GROWING OF NON-ORGANIC PLANT CULTURES OVER WATERPROOF AND WET-WATER AREAS |
Non-Patent Citations (1)
| Title |
|---|
| 高阳等: "间作群体内土壤呼吸和硝化-反硝化作用研究", 《灌溉排水学报》 * |
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