CN105973963B - Hair clip DNA supports construction method and the application of dual signal molecule sensing interface - Google Patents
Hair clip DNA supports construction method and the application of dual signal molecule sensing interface Download PDFInfo
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- 238000001514 detection method Methods 0.000 claims abstract description 36
- 239000000523 sample Substances 0.000 claims abstract description 34
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims abstract description 27
- 229960000907 methylthioninium chloride Drugs 0.000 claims abstract description 27
- KTWOOEGAPBSYNW-UHFFFAOYSA-N ferrocene Chemical compound [Fe+2].C=1C=C[CH-]C=1.C=1C=C[CH-]C=1 KTWOOEGAPBSYNW-UHFFFAOYSA-N 0.000 claims abstract description 22
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
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- SJUCACGNNJFHLB-UHFFFAOYSA-N O=C1N[ClH](=O)NC2=C1NC(=O)N2 Chemical compound O=C1N[ClH](=O)NC2=C1NC(=O)N2 SJUCACGNNJFHLB-UHFFFAOYSA-N 0.000 claims description 3
- 230000005518 electrochemistry Effects 0.000 claims description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims 2
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- PZBFGYYEXUXCOF-UHFFFAOYSA-N TCEP Chemical compound OC(=O)CCP(CCC(O)=O)CCC(O)=O PZBFGYYEXUXCOF-UHFFFAOYSA-N 0.000 description 2
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- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3276—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a hybridisation with immobilised receptors
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
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Abstract
The invention discloses a kind of construction method of hair clip DNA support dual signal molecule sensing interface and its applications, the construction method of sensing interface be will contain the tumor markers aptamer I and the probe containing ferrocene of methylene blue probe tumor markers aptamer II be incubated for jointly after, modification in surface modification has the electrode surface of nanogold to obtain the final product；The hair clip DNA support dual signal molecule sensing interface stability that this method obtains is good, it is at low cost, sensing interface can detect two kinds of tumor markers respectively, two kinds of tumor markers can also be detected simultaneously on same interface, and have the characteristics that detection is simpler, detection limit is low, high sensitivity, high specificity.
Description
Technical field
The present invention relates to a kind of construction method of hair clip DNA support dual signal molecule sensing interface and with sensing circle
The electrochemical method of two kinds of tumor markers of the synchronous detection in face；Belong to electrochemica biological sensor field.
Background technique
Cancer base antigens c EA is a kind of proteoglycan compound, can be widely present in the Alimentary System of endoderm origin, be
One broad spectrum activity tumor markers tentatively judges the presence of tumour according to the content of CEA in serum, evaluates curative effect, it is thin to estimate cancer
Intracellular growth situation；MUC1 mucoprotein is a kind of glycoprotein of macromolecule, it is expressed in canceration surface epithelial cell Height Anomalies,
It is a kind of common tumor markers, the serum levels of MUC1 are also related to the grade malignancy of tumour.Therefore to both tumour marks
The detection of will object has highly important effect to the detective of cancer cell and detection.But in existing detection method, detection
Sensitivity inspection and concentration range need to be improved, and are distributed that detection efficiency is low, in order to preferably assess the development of disease,
The detection of both markers is particularly important, especially if two kinds of tumor markers can be detected simultaneously, will be mentioned significantly
High detection efficiency and accuracy.
Chinese patent (application No. is 201410181470.2) discloses a kind of while detecting two kinds of acute leukemia marks
The preparation method of the electrochemical sensor of object is specifically disclosed lysozyme reporter probe and gamma interferon reporter probe difference
It is stood in the solution containing TCEP, forms double-strand with opened disulfide bond and respectively with lysozyme aptamer and gamma interferon aptamer
Structure；Gold electrode is pre-processed, the gold electrode handled well is immersed in pretreated lysozyme reporter probe-aptamer double-strand
In gamma interferon reporter probe-aptamer double-strand mixed liquor, it is stored at room temperature overnight, uses secondary distilled water and cleaning solution later
Cleaning；Then electrode is immersed in the solution containing MCH, with enclosed-electrode, is cleaned later with secondary distilled water and cleaning solution；It will
Above-mentioned steps treated electrode is as working electrode and reference electrode, be connected on chem workstation electrode, with obtain can
For detecting electrochemical sensor while two kinds of acute leukemia marker lysozymes and gamma interferon.But its building
There is also following obvious disadvantages for sensor and method: 1, there is competition when two kinds of probes form double-strand with corresponding aptamer respectively
Reaction；The two kinds of hairpin structures formed are connected on electrode again there are competitive reaction, complex steps, and sensor properties are unstable；
2, there are four kinds of forms for the molecule for being connected on electrode: two kinds of probe molecules and two kinds of hairpin structure molecules, what is connected every time has
The quantity and ratio of the hairpin structure of effect are uncertain, poor repeatability, and two kinds of probe molecules are on the electrode formed detection
Interference；3, four kinds of nucleic acid molecules chains are used, the at high cost of sensor is caused.
Summary of the invention
Defect existing for method for existing detection tumor markers, the purpose of the invention is to provide a kind of behaviour
Make the method for building hair clip DNA support dual signal molecule sensing interface simple, reproducible, at low cost, sensing circle of building
Face stability is good, signal is strong, specificity is high, high sensitivity, and can be used for detecting one kind or detect two kinds of tumor markers simultaneously
Object.
Second object of the present invention is to be to provide a kind of application of hair clip DNA support dual signal molecule sensing interface,
The biosensor may be respectively used for two kinds of tumor markers of detection, can also detect two kinds of tumours simultaneously on same interface
Marker, and have the characteristics that detection is simpler, detection limit is low, high sensitivity, high specificity.
In order to achieve the above technical purposes, the present invention provides a kind of hair clip DNA support dual signal molecule sensing interfaces
Construction method, method includes the following steps:
(1) pass through sedimentation modified nano gold in electrode surface；
(2) the tumor markers aptamer II of the tumor markers aptamer I of the probe containing methylene blue and the probe containing ferrocene exists
At a temperature of 25~40 DEG C, in light protected environment, mixing is incubated for 2~4h in the solution, obtains being incubated for mixed solution；
The tumor markers aptamer I of the probe containing methylene blue and swelling for the probe containing ferrocene in the incubation mixed solution
Tumor markers aptamer II concentration is respectively 1~10 μM and 1~10 μM；
(3) in light protected environment, the electrode of surface finish nano gold is placed in the incubation mixed solution, incubation 14~
After 16h, it is rinsed with water；
(4) electrode of (3) treated surface finish nano gold is placed in MCH solution closing to get hair clip DNA branch again
Support dual signal molecule sensing interface.
Preferred scheme, electrode described in (1) are placed in the chlorauric acid solution that concentration is 2~5mM, and it is heavy to carry out constant potential
Product, current potential are that -0.4V arrives -0.1V, and sedimentation time is 150~200s, obtains surface finish nano gold electrode.It deposits on the electrode
After one layer of nanogold particle, electrode specific surface increases, and is on the one hand conducive to combine aptamer, on the other hand can greatly enhance signal
Intensity has higher detection limit.
Preferred scheme, hair clip DNA support dual signal molecule sensing interface includes the tumour containing methylene blue (MB) probe
The marker aptamer I and tumor markers aptamer II for containing ferrocene (Fc) probe.
More preferably scheme, the tumor markers aptamer I of the probe containing methylene blue and the tumor-marker of the probe containing ferrocene
Object aptamer II modifies the electrode surface in surface finish nano gold after being incubated for jointly.
Further preferred scheme, tumor markers aptamer I are CEA aptamer.
Further preferred scheme, tumor markers aptamer II are MUC1 aptamer.
The hair clip DNA support dual signal molecule sensing interface of method building of the invention is acted on accordingly using aptamer
Albumen has higher specificity and discernment, the sensitivity of detection higher；Two kinds of signaling molecules are introduced simultaneously, are examined respectively
It is not interfere with each other when surveying two kinds of different tumor markers, while detecting, detection limit is low, high-efficient.
The present invention also provides the applications of the hair clip DNA support dual signal molecule sensing interface of the method building, by institute
It states hair clip DNA support dual signal molecule sensing interface and is applied to the corresponding tumor markers I of detection tumor markers aptamer I, or
Person detects the corresponding tumor markers II of tumor markers aptamer II, or to detect tumor markers aptamer I simultaneously corresponding swollen
The corresponding tumor markers II of tumor markers I and tumor markers aptamer II.Sensor of the invention can detect respectively CEA and
MUC1, and CEA and MUC1 can be detected simultaneously, and the detection of two kinds of tumor markers respectively corresponds two kinds of unlike signals
The variable quantity of molecule, specificity, independence and stability are higher.
Hair clip DNA support dual signal molecule sensing interface is used to detect tumor markers I by preferred scheme, according to tumour
The variable quantity of front and back methylene blue electrochemical signals is added to detect the content of tumor markers I in marker I；
Alternatively, being used to detect tumor markers II for hair clip DNA support dual signal molecule sensing interface, according to tumor-marker
The variable quantity of front and back ferrocene electrochemical signals is added to detect the content of tumor markers II in object II；
Alternatively, being used for hair clip DNA support dual signal molecule sensing interface to detect tumor markers I and tumor-marker simultaneously
Object II, tumor markers I and tumor markers II are added simultaneously, and front and back methylene blue electrochemistry is added according to tumor markers I
The variable quantity of signal detects the content of tumor markers I, according to tumor markers II front and back ferrocene electrochemical signals is added
Variable quantity detect the content of tumor markers II.
More preferably scheme, tumor markers I are CEA.
More preferably scheme, tumor markers II are MUC1.
The tumor markers aptamer I of the probe containing methylene blue of the invention and the tumor markers aptamer of the probe containing ferrocene
II can be obtained by existing conventional method screening technique.Or directly by being commercially available, such as raw work bioengineering Shanghai
(share) Co., Ltd.The tumor markers aptamer I for the probe containing methylene blue that the present invention uses and swelling for the probe containing ferrocene
The one end tumor markers aptamer II is modified with sulfydryl, the main nanometer gold bonding for passing through sulfydryl and electrode surface, by corresponding aptamer
It is fixed on electrode surface.
The molecular probe that the present invention uses, Fc are reducing substances, and square wave is measured in PBS, are had in 0.35V or so bright
The peak of aobvious Fc；It, can be with the amount of quantitative detection MUC1 according to the variable quantity of square wave current.MB is also reducing substances, in PBS
Square wave is measured, has the peak of apparent methylene blue in -0.27V or so；It, can be with quantitative detection according to the variable quantity of square wave current
The amount of CEA.
Compared with the prior art, technical solution of the present invention bring advantageous effects:
1) the construction method key of hair clip DNA of the invention support dual signal molecule sensing interface is two kinds containing difference
After the tumor markers aptamer of molecular probe is incubated for hybridization, then modify to the electrode surface of surface finish nano gold.This method can
To realize that two kinds of tumor markers aptamers exist by two kinds of tumor markers aptamer concentrations in simple adjustment incubation hybrid process
The modification ratio of electrode surface, two kinds of aptamers are hybridized, and competitive relation are not present, formation one is whole after two kinds of molecule hybridization
Body is connected on electrode also there is no competition, and controllability is strong, and the sensing interface modifier stable components constructed.Complete gram
It detects two kinds of tumor markers electrochemical sensors while clothes in the prior art there are stability and is poor, poor repeatability scarce
It falls into.
2) the hair clip DNA support dual signal molecule sensing interface constructed by means of the present invention, two kinds contain different molecular
The tumor markers aptamer of probe is hybridized by being incubated for jointly, then modifies the electrode surface in surface modification gold, is connected on electrode
Molecule there are two kinds of forms: individually detect a kind of aptamer of substance and support the aptamer of dual signal, the quantity ratio of two kinds of aptamers
Example be it is certain, the molecule connect on the electrode can detect marker, not interfere with substance, and detection limit is low, sensitive
Du Genggao.
3) hair clip DNA of the invention support dual signal molecule sensing interface construction method is simple, reproducible, and only with
Two kinds of nucleic acid molecules chains, cost are relatively lower.
4) probe molecule, that is, aptamer of hair clip DNA of the invention support dual signal molecule sensing interface modification, can directly use
To detect marker.
5) hair clip DNA of the invention support dual signal molecule sensing interface passes through modification tumor markers aptamer
(aptamer) to act on tumor markers, while tumor-marker is detected according to the size of the electrochemical signals of molecular probe
The content of object has the characteristics that high sensitivity, detection limit be low, high specificity.
6) it is suitable to have modified two kinds of different tumor markers simultaneously for hair clip DNA of the invention support dual signal molecule sensing interface
Body and two kinds of signaling molecules are introduced, can be used for detecting a kind of tumor markers can also detect two kinds of tumor-markers simultaneously
Object realizes detection while based on the same two kinds of tumor markers in interface, and with detecting, simpler, detection limit is lower, sensitive
The characteristics of degree is high, high specificity, and when detecting two kinds of tumor markers simultaneously, it does not interfere with each other, detection limit is low, high-efficient.
7) hair clip DNA support dual signal molecule sensing interface of the invention introduces aptamer, and stability is good, and aptamer and corresponding
Tumor markers can form stable condensate, high sensitivity, detection limit are low.And aptamer is compared to antibody, processes
Cheng Gengwei is simple, and is easily-synthesized, easily modification signaling molecule, it can be more readily detected and goes out required electrochemical signals.
8) two kinds of aptamer sequences that hair clip DNA support dual signal molecule sensing interface of the present invention introduces are variable, aptamer replacement
It can be generalized to other corresponding markers of detection after sequence, such as polypeptide, DNA, RNA enable sensor to obtain more extensively
Application, have certain generality.
Detailed description of the invention
[Fig. 1] is the process schematic for constructing sensing interface；
[Fig. 2] is the cv characterization of electrode before and after electro-deposition
[Fig. 3] is that the EIS of sensing interface building process is characterized；
[Fig. 4] is the SWV figure after a series of MUC1 of various concentrations is added in the sensing interface surface of building；[Fig. 5] is
The linear relationship chart of MUC1 in certain concentration range.
Specific embodiment
Following embodiment is intended to further illustrate the content of present invention, rather than limits the protection model of the claims in the present invention
It encloses.
(1) method of the biosensor constructed on the electrode, steps are as follows:
Two can be mixed at 25-40 DEG C into lower dark with 10 μM of DNA1-MB and 10 μM of DNA2-Fc of partial complementarity
It is incubated for 3h, obtains DNA mixed liquor.
Electrode carries out potentiostatic electrodeposition in the chlorauric acid solution of 0.5mM, and current potential is -0.25V, sedimentation time 180s,
Surface obtains one layer of nanogold particle, this layer of nanogold can fixed adaptation body in a subsequent step, while playing signal and putting
Big effect.At room temperature, it is modified on electrode in 10 μM of DNA mixture dark and is incubated for 15h, then rinsed, gone with secondary water
Except the remaining DNA being not decorated, be then placed in MCH by electrode, be added MCH purpose be in order on enclosed-electrode not
The site of reaction and the DNA for making modification are in rising structure.Due to the light-exposed easy decomposition of methylene blue, so all operations
Process is carried out under dark condition.
To the quantitative analysis of CEA, steps are as follows:
(2) CEA for preparing various concentration respectively, is sequentially added on the biosensor constructed above, due to
DNA1-MB Partial Fragment is the aptamer of CEA, it can form stable paradigmatic structure with CEA.So that DNA1-MB
Hairpin structure is opened, so that MB is farther apart from electrode, according to the variable quantity of methylene blue signal on DNA-MB come quantitative analysis
CEA。
To the quantitative analysis of MUC1, steps are as follows:
(3) MUC1 of various concentration is respectively configured, is sequentially added on the biosensor constructed above, due to
DNA2-Fc is the aptamer of MUC1, it can form stable paradigmatic structure to drop in solution, so that electric with MUC1
Extremely upper Fc signal reduces.According to the variable quantity of ferrocene signal come quantitative analysis MUC1.
(4) as follows the step of synchronous detection with MUC1's to CEA:
The methylene blue and ferrocene signal for detecting the sensor prepared are initial signal, while various concentration is added
CEA and MUC1 is quantified by the variable quantity of methylene blue signal come quantitative detection CEA by the variable quantity of ferrocene signal
Detect MUC1.
The reduction peak of the gold of 0.8-1V occurs after electro-deposition nanogold as can be seen from Figure 2, and, table bigger than naked gold electrode
Bright nanogold successful deposition has arrived on electrode.
As can be seen from Figure 3 with more, the impedance increase of electrode surface building, the successful structure of surface biological sensor
It builds.
From Fig. 4 as the amount of the tumor markers MUC1 of addition gradually increases, response signal is gradually increased.
MUC1 concentration is bigger in certain concentration range as can be seen from Figure 5, and the variable quantity of electric current is bigger, reaches certain
It can tend to balance after one concentration, there is its good linear relationship in the low concentration range.
Comparative example 1
The specific experiment of control group is as follows:, will according to the method for publication (application No. is 201410181470.2)
Two kinds of probes are stood in the solution containing TCEP respectively, form double-strand with opened disulfide bond and respectively with respective aptamer
Structure；Gold electrode is pre-processed, the gold electrode handled well is immersed in the mixing of pretreated two kinds of probes-aptamer double-strand
In liquid, it is stored at room temperature overnight, is cleaned later with secondary distilled water and cleaning solution；Then electrode is immersed in the solution containing MCH,
With enclosed-electrode, cleaned later with secondary distilled water and cleaning solution；Using above-mentioned steps treated electrode as working electrode, and
Reference electrode is connected on chem workstation electrode, to obtain can be used for the electrochemical sensing of two kinds of markers while detection
Device.It is added without any detectable substance, the methylene blue and ferrocene initial signal of the sensor prepared are detected with square wave voltammetry,
And the ratio of two kinds of signal magnitudes is calculated, 5 parallel laboratory tests are carried out respectively, obtain the relative standard deviation of 5 experiments.
The specific experiment of experimental group group is as follows: according to methods described herein, by the tumor markers of the probe containing methylene blue
The tumor markers aptamer II of aptamer I and the probe containing ferrocene in light protected environment, is mixed in the solution at a temperature of 25~40 DEG C
It closes and is incubated for 2~4h, obtain the tumor markers aptamer I of the probe containing methylene blue and the tumor markers aptamer of the probe containing ferrocene
The mixed solution of II；Gold electrode is pre-processed, and one layer of nanogold of electro-deposition, at room temperature, modifying DNA mixes on electrode
It is incubated for 15h in object dark, is rinsed with secondary water；Then electrode is immersed in the solution containing MCH, with enclosed-electrode, Zhi Houyong
Secondary distilled water cleaning；Using above-mentioned steps treated electrode as working electrode and reference electrode, chemistry is connected to electrode
On work station, to obtain can be used for the electrochemical sensor of two kinds of markers while detection.It is added without any detectable substance, uses square wave
The methylene blue and ferrocene initial signal for the sensor that voltammetry detection prepares, and the ratio of two kinds of signal magnitudes is calculated
Value carries out 5 parallel laboratory tests respectively, obtains the relative standard deviation of 5 experiments.
| Experiment number | 1 | 2 | 3 | 4 | 5 | RSD (%) |
| (control group) IMB/IFc | 1.18 | 0.91 | 1.13 | 1.08 | 0.99 | 10.25 |
| (experimental group) IMB/IFc | 1.64 | 1.6 | 1.62 | 1.59 | 1.63 | 1.28 |
It can be obtained by experimental result it is found that control group and the RSD of experimental group are respectively 10.25%, 1.28%, it follows that
The sensing interface modifier ingredient of this method building is more stable, and repeatability is higher.
Claims (8)
1. the construction method of hair clip DNA support dual signal molecule sensing interface, it is characterised in that: the following steps are included:
(1) pass through sedimentation modified nano gold in electrode surface；
(2) the tumor markers aptamer II of the tumor markers aptamer I of the probe containing methylene blue and the probe containing ferrocene 25~
At a temperature of 40 DEG C, in light protected environment, mixing is incubated for 2~4h in the solution, obtains being incubated for mixed solution；The incubation mixing
The tumor markers aptamer II concentration of the tumor markers aptamer I of the probe containing methylene blue and the probe containing ferrocene is distinguished in solution
For 1~10 μM and 1~10 μM；
(3) in light protected environment, the electrode of surface finish nano gold is placed in the incubation mixed solution, is incubated for 14~16h
Afterwards, it is rinsed with water；
(4) electrode of (3) treated surface finish nano gold is placed in MCH solution again close supported to get hair clip DNA it is double
Signaling molecule sensing interface.
2. the construction method of hair clip DNA support dual signal molecule sensing interface according to claim 1, it is characterised in that:
(1) electrode described in be placed in concentration be 2~5mM chlorauric acid solution in, carry out potentiostatic electrodeposition, current potential be -0.4V to -
0.1V, sedimentation time are 150~200s, obtain surface finish nano gold electrode.
3. the construction method of hair clip DNA support dual signal molecule sensing interface according to claim 1, it is characterised in that:
The described hair clip DNA support dual signal molecule sensing interface includes the tumor markers aptamer I of the probe containing methylene blue and containing two
The tumor markers aptamer II of luxuriant iron probe.
4. the construction method of hair clip DNA support dual signal molecule sensing interface according to claim 3, it is characterised in that:
The tumor markers aptamer I of the probe containing methylene blue and the tumor markers aptamer II of the probe containing ferrocene are by being incubated for
Electrode surface of the common modification in surface finish nano gold afterwards.
5. the construction method of hair clip DNA support dual signal molecule sensing interface according to claim 4, it is characterised in that:
The tumor markers aptamer I is CEA aptamer；The tumor markers aptamer II is MUC1 aptamer.
6. the application of the hair clip DNA support dual signal molecule sensing interface of any one of Claims 1 to 5 the method building,
It is characterized in that: being applied to the corresponding tumor markers I of detection tumor markers aptamer I, or detection tumor markers aptamer II
Corresponding tumor markers II, or to detect the corresponding tumor markers I of tumor markers aptamer I and tumor markers simultaneously suitable
The corresponding tumor markers II of body II.
7. the application of hair clip DNA support dual signal molecule sensing interface according to claim 6, it is characterised in that:
Hair clip DNA support dual signal molecule sensing interface is used to detect tumor markers I, before being added according to tumor markers I
The variable quantity of methylene blue electrochemical signals detects the content of tumor markers I afterwards；
Alternatively, being used to detect tumor markers II for hair clip DNA support dual signal molecule sensing interface, according to tumor markers II
The variable quantity of front and back ferrocene electrochemical signals is added to detect the content of tumor markers II；
Alternatively, being used for hair clip DNA support dual signal molecule sensing interface to detect tumor markers I and tumor markers simultaneously
II, tumor markers I and tumor markers II are added simultaneously, and front and back methylene blue electrochemistry is added according to tumor markers I and believes
Number variable quantity detect the content of tumor markers I, front and back ferrocene electrochemical signals are added according to tumor markers II
Variable quantity detects the content of tumor markers II.
8. the application of hair clip DNA support dual signal molecule sensing interface according to claim 7, it is characterised in that: described
Tumor markers I be CEA；The tumor markers II is MUC1.
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| CN106841346A (en) * | 2016-12-22 | 2017-06-13 | 山东理工大学 | A kind of palm aflatoxin fast detector based on Internet of Things |
| CN107290406B (en) * | 2017-06-20 | 2019-05-24 | 中南大学 | For detecting the ferrocene of miRNA and the magnetic ball nano-complex of methylene blue double labeling and preparation method thereof |
| CN108445067B (en) * | 2018-05-04 | 2020-07-03 | 安徽师范大学 | Double-signal enzyme-free signal amplification RNA nano biosensor, preparation method and application thereof |
| CN110873745A (en) * | 2018-09-04 | 2020-03-10 | 南京大学 | Novel method for detecting acute myelogenous leukemia marker Siglec-5 |
| CN109738500B (en) * | 2019-02-20 | 2019-08-06 | 青岛大学 | The preparation method of nano-complex and its label-free aptamer electrochemistry gamma interferon sensor |
| CN110849867B (en) * | 2019-12-06 | 2022-03-04 | 东北师范大学 | Electroluminescent sensor based on double-hairpin structure and construction method and application thereof |
| CN113219031A (en) * | 2021-03-15 | 2021-08-06 | 皖南医学院 | DNA biped walker signal amplifier, nano-electrode based biosensor and methods of use and applications thereof |
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