CN105973665A - Diarrhetic shellfish poison sample high-flux preprocessing device - Google Patents
Diarrhetic shellfish poison sample high-flux preprocessing device Download PDFInfo
- Publication number
- CN105973665A CN105973665A CN201610290180.0A CN201610290180A CN105973665A CN 105973665 A CN105973665 A CN 105973665A CN 201610290180 A CN201610290180 A CN 201610290180A CN 105973665 A CN105973665 A CN 105973665A
- Authority
- CN
- China
- Prior art keywords
- peristaltic pump
- power supply
- pure water
- methanol
- proportioning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a diarrhetic shellfish poison sample high-flux preprocessing device. The device comprises a control circuit, a switch power supply, a motor speed regulation plate, a peristaltic pump, a power supply interface, power supply sockets, a communication interface, a liquid tube interface, corresponding connecting tubes and a cabinet, wherein the power supply interface is connected with the switch power supply to input commercial power in order to supply power for the device; the power supply sockets are connected to the control circuit, and supply power for independent working devices; the communication interface is connected to the control circuit; the motor speed regulation plate and the control circuit are fixedly arranged above the switch power supply, and are connected with corresponding peristaltic pumps to control the working of the peristaltic pumps; the peristaltic pumps are fixedly arranged on the cabinet through a pedestal; the inner side of the front panel liquid tube interface is connected with the peristaltic pumps through Teflon tubes, and the interface is fixed through a band; and the outer side of the liquid tube interface leads out liquid flow through a Teflon tube. The device realizes high-flux preprocessing of a shellfish meat sample in the diarrhetic shellfish poison detection process, and improves the sample preprocessing efficiency.
Description
Technical field
The present invention relates to Sample Pretreatment Technique, particularly relate to a kind of for detecting the diarrhoeal poison of marine aquatic product
The shellfish meat sample high flux pretreating device of element.
Background technology
The diarrhoeal toxin of shellfish, is the fat-soluble multi-ring ethers bioactive substance produced by poisonous red tide algae.
In red tide environment, the toxic algae if shellfish ingests in a large number, toxin can be in its internal a large amount of enrichments.If people
Eat this shellfish and relevant food by mistake, have greater risk can cause poisoning, even result in death.At present, shellfish
During the diarrhoeal Mycotoxin identification of class, mostly by the pre-treatment completing shellfish meat sample manually.And artificial hand
Move and carry out needing professional to operate during pre-treatment, in addition it is also necessary to a large amount of professional equipments, operating environment is had
Particular/special requirements etc. limit.Meanwhile, once a sample can only be operated, limited efficacy.To sum up, people
The manual pre-treatment of work is inefficient, it is more difficult to adapt to the demand of Site Detection.So existing at marine aquatic product toxin
Field detection field is accomplished by a kind of operation and carries simplicity, and treatment effeciency is high, adapts to the shellfish of Site Detection demand
Class diarrhoeal toxin sample pretreating device.
Summary of the invention
Present invention aims to the deficiencies in the prior art, it is provided that a kind of diarrhoeal toxin sample of shellfish is high
Flux pretreating device.
The purpose of the present invention is realized by techniques below scheme: before a kind of shellfish diarrhoeal toxin sample high flux
Reason device, including: chassis base, case front panel, cabinet rear board and cabinet top cover, described cabinet top
The cross section of lid is U-shaped, and case front panel, cabinet rear board and cabinet top cover are each attached on chassis base；
It is characterized in that, described case front panel be fixed with filtration injection port group, filter outlet group, perfusion entrance,
Priming outlet, pure water inlet, pure water liquid outlet, methanol inlet and the interface of methanol liquid outlet；
Described cabinet rear board is fixed with two power supply bases, AC power interface, weighing communication interface and string
Mouth communication interface；Power supply base provides working power for external homogenizer and centrifuge；
Fixing perfusion peristaltic pump, filtration peristaltic pump group, pure water proportioning peristaltic pump, methanol on described chassis base
Proportioning peristaltic pump and Switching Power Supply；Electric machine speed regulation plate and control circuit is fixed in described Switching Power Supply；Described control
Circuit processed includes singlechip controller, relay group and power module；Three pins of AC power interface divide
The most corresponding with the live wire of Switching Power Supply, zero line and ground wire input connected；The outfan of Switching Power Supply connects electricity
Source module, power module is perfusion peristaltic pump, filters peristaltic pump group, pure water proportioning peristaltic pump, methanol proportioning
Peristaltic pump, singlechip controller, relay group and electric machine speed regulation plate are powered；Power module is defeated by Switching Power Supply
The high voltage gone out is reduced to the running voltage needed for singlechip controller and speed adjusting module, to support that it is normal
Work.Perfusion peristaltic pump, filtration peristaltic pump group, pure water proportioning peristaltic pump, methanol proportioning peristaltic pump and power supply
Socket is all connected with relay group, and pure water proportioning peristaltic pump and methanol proportioning peristaltic pump are connected with electric machine speed regulation plate；
Relay group, electric machine speed regulation plate, weighing communication interface and serial communication interface are all connected with singlechip controller；
The entrance correspondence of described filtration peristaltic pump group connects filtration injection port group, and outlet correspondence connection filters out sample
Mouth group；The entrance of described perfusion peristaltic pump connects perfusion entrance, and outlet connects priming outlet；Described pure water is joined
Connecting pure water inlet than the entrance of peristaltic pump, outlet connects pure water liquid outlet；Described methanol proportioning peristaltic pump
Entrance connect methanol inlet, outlet connect methanol liquid outlet；
Described singlechip controller accepts the instruction of host computer by serial communication interface, according to required extract
The rotating speed of ratio-dependent electric machine speed regulation plate, gather the weighing result of external electronic scale by weighing communication interface,
Determine pure water proportioning peristaltic pump and the working time of methanol proportioning peristaltic pump；Control the power supply being connected with homogenizer
The make-and-break time of socket, thus control the homogenizing time of sample, by perfusion peristaltic pump perfusion sample to centrifugal
Pipe；Control the make-and-break time of the power supply base being connected with centrifuge, thus control the centrifugation time of sample, logical
Filter the supernatant after peristaltic pump group filter centrifugation, complete pre-treatment.
Further, described filtration injection port group, filter outlet group, perfusion entrance, priming outlet, pure
Water inlet, pure water liquid outlet, methanol inlet and methanol liquid outlet by external teflon pipe, straight-through connect
Head, pagoda joint or filter carry out drain to liquid stream.
The present invention, relative to prior art, has the advantages that the diarrhoeal toxin sample of shellfish of the present invention
High flux pretreating device, it is achieved that locate before the diarrhoeal toxin sample of shellfish in aquatic products Mycotoxin identification field
The automatization of reason process and high pass quantify.Apparatus of the present invention solve the some shortcomings of pre-treatment manually,
Easy and simple to handle, only need to prepare the shellfish meat sample that need to detect and required reagent, high flux design simultaneously improves inspection
The efficiency surveyed, meets Site Detection requirements of one's work.Apparatus of the present invention can be widely used for the diarrhoeal toxin of shellfish
The association area of detection pre-treatment.
Accompanying drawing explanation
Fig. 1 is apparatus of the present invention cut-away views；
Fig. 2 is front end of the present invention external structure schematic diagram；
Fig. 3 is rear end of the present invention external structure schematic diagram；
In figure, chassis base 1, perfusion peristaltic pump 2, filter peristaltic pump group 3, pure water proportioning peristaltic pump 4,
Methanol proportioning peristaltic pump 5, Switching Power Supply 6, electric machine speed regulation plate 7, control circuit 8, relay group 9, power supply
Module 10, singlechip controller 11, cabinet rear board 12, power supply base 13, AC power interface 14,
Weigh communication interface 15, serial communication interface 16, case front panel 17, filter injection port group 18, filtration
Outlet group 19, perfusion entrance 20, priming outlet 21, pure water inlet 22, pure water liquid outlet 23, first
Alcohol inlet 24, methanol liquid outlet 25, cabinet top cover 26.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail, but is not to limit this
Invention.
As it is shown in figure 1, the shellfish of the present invention diarrhoeal toxin sample high flux pretreating device, including: machine
Bottom seat 1, perfusion peristaltic pump 2, filtration peristaltic pump group 3, pure water proportioning peristaltic pump 4, methanol proportioning are wriggled
Pump 5, Switching Power Supply 6, electric machine speed regulation plate 7, control circuit 8, relay group 9, power module 10, list
Sheet machine controller 11, cabinet rear board 12, power supply base 13, AC power interface 14, weighing communication connect
Mouthfuls 15, serial communication interface 16, case front panel 17, filter injection port group 18, filter outlet group 19,
Perfusion entrance 20, priming outlet 21, pure water inlet 22, pure water liquid outlet 23, methanol inlet 24,
Methanol liquid outlet 25 and cabinet top cover 26.
As in figure 2 it is shown, case front panel 17 be fixed with filtration injection port group 18, filter outlet group 19,
Perfusion entrance 20, priming outlet 21, pure water inlet 22, pure water liquid outlet 23, methanol inlet 24 and
The interface of methanol liquid outlet 25；Case front panel 17 and cabinet top cover 26 are screwed in chassis base
On 1.
As it is shown on figure 3, cabinet rear board 12 be fixed with two power supply bases 13, AC power interface 14,
Weigh communication interface 15 and serial communication interface 16；Cabinet rear board 12 and cabinet top cover 26 are solid by screw
On chassis base 1；Power supply base 13 provides working power for external homogenizer and centrifuge so that it is
Can be operated according to the working time set；AC power interface 14 is powered for device, it is ensured that each portion of device
Parallel circuit normally works；Weighing communication interface 15 can be by incoming for the weighing result of external electronic scale Single-chip Controlling
In device, the work further for system provides parameter reference.
Chassis base 1, case front panel 17, cabinet rear board 12 and cabinet top cover 26 are connected by screw,
The external structure of constituent apparatus；Perfusion peristaltic pump 2, filtration peristaltic pump group 3, pure water proportioning peristaltic pump 4 and first
Alcohol proportioning peristaltic pump 5 is fixed on chassis base 1 by base；Switching Power Supply 6 is bolted on machine
On bottom seat 1, electric machine speed regulation plate 7 and control circuit 8 are fixed on Switching Power Supply 6 by copper post；Hand over
Three pins of stream power interface 14 are respectively by the live wire of wire with Switching Power Supply 6, zero line and ground wire input
End correspondence is connected；Power supply base 13 live wire end relay group 9 on control circuit plate 8 and alternating current power supply
The live wire end of interface 14 is connected, and zero line is joined directly together by wire with the zero line side of AC power interface 14；
Weigh communication interface 15 to be connected with singlechip controller 11 by Du Pont's line with serial communication interface 16；Motor
Speed adjusting plate 7 obtains running voltage from power module 10, and input and singlechip controller 11 are by Du Pont's line
Being connected, outfan is connected with pure water proportioning peristaltic pump 4 and methanol proportioning peristaltic pump 5 respectively；Single-chip Controlling
Device 11 regulates the rotating speed of motor by the PWM ripple of output different duty.Perfusion peristaltic pump 2, filtration
Peristaltic pump group 3, pure water proportioning peristaltic pump 4 and methanol proportioning peristaltic pump 5 by straight coupling and teflon pipe with
Filter injection port group 18, filter outlet group 19, perfusion entrance 20, priming outlet 21, pure water inlet
22, pure water liquid outlet 23, methanol inlet 24 are connected with the inner side of the interface of methanol liquid outlet 25, wherein
Straight coupling needs band or glue to fix with the junction of peristaltic pump, by teflon pipe drain outside interface,
Straight coupling, pagoda joint and external filter can be connected when needing；Parts on chassis base 1 and two
After side panel has been fixed, cover cabinet top cover 26, the assembling of finishing device.
The workflow of the present invention is as follows:
Prepare to strip and remove the shellfish meat sample of chip tissue, prepare enough methanol reagent, pure water and cleanings
Liquid.Two power supply bases 13, AC power interface 14, weigh communication interface 15 and serial communication interface 16
It is connected with homogenizer, centrifuge, power supply, electronic scale and host computer respectively.After having connected, open friendship
Power supply on stream power interface 14, operates host computer, confirms to communicate successfully with slave computer.After communicating successfully,
Weigh shellfish meat weight, after host computer reads weighing result, set extract consumption.Need before proportioning joining
Carrying out rinse than pipeline, singlechip controller 11 controls pure water proportioning peristaltic pump 4 and methanol proportioning is wriggled afterwards
Pump 5 proportioning 80% methanol, during by the work that controls pure water proportioning peristaltic pump 4 and methanol proportioning peristaltic pump 5
Between control flow.After completing the proportioning of 80% methanol, PC control power supply base 13 connects homogenizer,
Shellfish meat sample and 80% methanol extraction liquid are rubbed and be sufficiently mixed.After having mixed, by external filter,
Singlechip controller 11 is used to control perfusion peristaltic pump 2 by shellfish meat suspension perfusion to 2mL centrifuge tube.Fill
After having noted, PC control power supply base 13 connects centrifuge, and shellfish meat sample perfusion completed is centrifuged
5min.After being centrifuged, the drainage tube being connected with filtration injection port group 18 is inserted the supernatant in centrifuge tube,
Singlechip controller 11 controls to filter peristaltic pump group 3 and extracts supernatant from filtering injection port group 18, is filtering
Outlet group 19 collects the extract obtained after connecting filter paper.After having extracted, use clear water to proportioning, filling
Note and filter pipeline are carried out.So far, shellfish meat sample pretreatment process completes in experiment.
Claims (2)
1. a shellfish diarrhoeal toxin sample high flux pretreating device, including: chassis base (1), machine
Case front panel (17), cabinet rear board (12) and cabinet top cover (26), described cabinet top cover (26)
Cross section is U-shaped, and case front panel (17), cabinet rear board (12) and cabinet top cover (26) are the most fixing
On chassis base (1)；It is characterized in that, described case front panel (17) is fixed with filtration injection port group
(18), filter outlet group (19), perfusion entrance (20), priming outlet (21), pure water inlet (22),
Pure water liquid outlet (23), methanol inlet (24) and the interface of methanol liquid outlet (25)；
Described cabinet rear board (12) be fixed with two power supply bases (13), AC power interface (14),
Weigh communication interface (15) and serial communication interface (16)；Power supply base (13) be external homogenizer and from
Scheming provides working power；
Upper fixing perfusion peristaltic pump (2) of described chassis base (1), filtration peristaltic pump group (3), pure water proportioning
Peristaltic pump (4), methanol proportioning peristaltic pump (5) and Switching Power Supply (6)；Described Switching Power Supply (6) is upper solid
Determine electric machine speed regulation plate (7) and control circuit (8)；Described control circuit (8) include singlechip controller (11),
Relay group (9) and power module (10)；Three pins of AC power interface (14) respectively with switch
The live wire of power supply (6), zero line are connected with ground wire input correspondence；The outfan of Switching Power Supply (6) connects
Power module (10), power module (10) is perfusion peristaltic pump (2), filters peristaltic pump group (3), pure water
Proportioning peristaltic pump (4), methanol proportioning peristaltic pump (5), singlechip controller (11), relay group (9)
Power with electric machine speed regulation plate (7)；Perfusion peristaltic pump (2), filtration peristaltic pump group (3), pure water proportioning are wriggled
Pump (4), methanol proportioning peristaltic pump (5) and power supply base (13) are all connected with relay group (9), pure
Water proportioning peristaltic pump (4) and methanol proportioning peristaltic pump (5) are connected with electric machine speed regulation plate (7)；Relay group
(9), electric machine speed regulation plate (7), weigh communication interface (15) and serial communication interface (16) is equal and monolithic
Machine controller (11) connects；
The entrance correspondence of described filtration peristaltic pump group (3) connects filtration injection port group (18), and outlet correspondence is even
Take over filter outlet group (19)；The entrance of described perfusion peristaltic pump (2) connects perfusion entrance (20), outlet
Connect priming outlet (21)；The entrance of described pure water proportioning peristaltic pump (4) connects pure water inlet (22),
Outlet connects pure water liquid outlet (23)；The entrance of described methanol proportioning peristaltic pump (5) connects methanol inlet
(24), outlet connects methanol liquid outlet (25)；
Described singlechip controller (11) accepts the instruction of host computer, root by serial communication interface (16)
According to the rotating speed of ratio-dependent electric machine speed regulation plate (7) of required extract, adopt by weighing communication interface (15)
Collect the weighing result of external electronic scale, determine pure water proportioning peristaltic pump (4) and methanol proportioning peristaltic pump (5)
Working time；Control the make-and-break time of the power supply base (13) being connected with homogenizer, thus control sample
Homogenizing time, by perfusion peristaltic pump (2) perfusion sample to centrifuge tube；Control the confession being connected with centrifuge
The make-and-break time of electrical socket (13), thus control the centrifugation time of sample, by filtering peristaltic pump group (3)
Supernatant after filter centrifugation, completes pre-treatment.
A kind of shellfish diarrhoeal toxin sample high flux pretreating device the most according to claim 1, its
It is characterised by, described filtration injection port group (18), filtration outlet group (19), perfusion entrance (20), filling
Outpour mouth (21), pure water inlet (22), pure water liquid outlet (23), methanol inlet (24) and methanol
Liquid stream is drawn by liquid outlet (25) by external teflon pipe, straight coupling, pagoda joint or filter
Stream.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610290180.0A CN105973665B (en) | 2016-05-04 | 2016-05-04 | The diarrhoeal toxin sample high throughput pretreating device of shellfish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610290180.0A CN105973665B (en) | 2016-05-04 | 2016-05-04 | The diarrhoeal toxin sample high throughput pretreating device of shellfish |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105973665A true CN105973665A (en) | 2016-09-28 |
| CN105973665B CN105973665B (en) | 2018-10-12 |
Family
ID=56993764
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610290180.0A Expired - Fee Related CN105973665B (en) | 2016-05-04 | 2016-05-04 | The diarrhoeal toxin sample high throughput pretreating device of shellfish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105973665B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112665946A (en) * | 2021-01-13 | 2021-04-16 | 浙江大学 | Portable integrated instrument for pretreatment and toxin detection of neurogenic shellfish sample |
| CN113295482A (en) * | 2021-04-01 | 2021-08-24 | 浙江大学 | Full-automatic toxin draws and detects integration instrument |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103278539A (en) * | 2013-06-07 | 2013-09-04 | 浙江大学 | Cell impedance sensor apparatus for detecting marine aquatic product diarrheal toxin, and method |
| CN103575893A (en) * | 2013-10-14 | 2014-02-12 | 广州市疾病预防控制中心 | Method for rapidly detecting shellfish toxin |
| WO2014181897A1 (en) * | 2013-05-06 | 2014-11-13 | 이도영 | Device for analyzing cells and monitoring cell culturing and method for analyzing cells and monitoring cell culturing using same |
| CN104777299A (en) * | 2015-03-13 | 2015-07-15 | 浙江大学 | Diarrhetic shellfish toxin high throughput detection device and method based on image analysis |
-
2016
- 2016-05-04 CN CN201610290180.0A patent/CN105973665B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014181897A1 (en) * | 2013-05-06 | 2014-11-13 | 이도영 | Device for analyzing cells and monitoring cell culturing and method for analyzing cells and monitoring cell culturing using same |
| CN103278539A (en) * | 2013-06-07 | 2013-09-04 | 浙江大学 | Cell impedance sensor apparatus for detecting marine aquatic product diarrheal toxin, and method |
| CN103575893A (en) * | 2013-10-14 | 2014-02-12 | 广州市疾病预防控制中心 | Method for rapidly detecting shellfish toxin |
| CN104777299A (en) * | 2015-03-13 | 2015-07-15 | 浙江大学 | Diarrhetic shellfish toxin high throughput detection device and method based on image analysis |
Non-Patent Citations (2)
| Title |
|---|
| HONGBO LI等: "Detection fo cardiovascular drugs and marine toxins using a multifunctional cell-based impedance biosensor sytstem", 《ANALYTICAL》 * |
| 苏凯麒等: "基于细胞阻抗传感器的腹泻型毒素检测系统设计与实现", 《传感技术学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112665946A (en) * | 2021-01-13 | 2021-04-16 | 浙江大学 | Portable integrated instrument for pretreatment and toxin detection of neurogenic shellfish sample |
| CN113295482A (en) * | 2021-04-01 | 2021-08-24 | 浙江大学 | Full-automatic toxin draws and detects integration instrument |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105973665B (en) | 2018-10-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105973665A (en) | Diarrhetic shellfish poison sample high-flux preprocessing device | |
| CN208626795U (en) | A kind of care product production natural plant protein extraction element | |
| CN206214490U (en) | A kind of dialysis concentrate of recycle heat prepares dialysis system | |
| CN207614657U (en) | A kind of fully-automatic thermostatic electrodialysis integrating device | |
| BR0202844A (en) | Method and apparatus as well as a connector and concentrate container unit for the preparation of solutions | |
| CN205974004U (en) | Water tank automatic reagent feeding system | |
| CN212067811U (en) | Traditional chinese medicine draws and transfers sour device | |
| CN108059204A (en) | A kind of industrial wastewater heats decontamination dosing device | |
| CN210410710U (en) | Clean phenate decomposition device | |
| CN207159223U (en) | A kind of bacteriologic test separator | |
| US10787376B2 (en) | Device for water disinfection by ultrafiltration | |
| CN108261924A (en) | A kind of preparation of the dehydration removing sodium device and hyponatremia slurry protein powder of animal blood plasma | |
| CN109851101A (en) | The low fluorine-containing mine water treatment system of one kind and equipment | |
| CN204897798U (en) | Black bean skin anthocyanidin intelligence extraction element | |
| CN109204826A (en) | A kind of plant protection drone convenient for spraying insecticide | |
| CN202762157U (en) | Filter device used between extraction tank and concentration tank | |
| CN214861757U (en) | A cryoconcentration equipment for oyster peptide draws | |
| CN204347017U (en) | In a kind of soy sauce, salinity on-line checkingi controls system | |
| CN203525365U (en) | Dendrobium candidum extracting device | |
| CN207342221U (en) | A kind of novel parallel concentration and evaporation instrument | |
| CN202446922U (en) | Full-automatic self-cleaning duplex filter | |
| CN214176469U (en) | Water collection type self-cleaning electric cabinet | |
| CN109307616A (en) | A kind of dialysis enrichment facility of antibody micro-example | |
| CN203373175U (en) | Extraction type domestic sewage treatment device | |
| CN207056019U (en) | A kind of traditional Chinese medicine extraction device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wang Ping Inventor after: Zhong Longjie Inventor after: Gu Chenlei Inventor after: Wan Zijian Inventor after: Su Kaiqi Inventor after: Zou Jichao Inventor after: Pan Yuxiang Inventor after: Qiu Xianxin Inventor after: Li Hongbo Inventor before: Wang Ping Inventor before: Wan Zijian Inventor before: Zhong Longjie Inventor before: Su Kaiqi Inventor before: Zou Jichao Inventor before: Pan Yuxiang Inventor before: Qiu Xianxin Inventor before: Li Hongbo |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181012 Termination date: 20190504 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |