CN105970191B - A kind of method for preparing anticoagulation zinc-oxide film on copper surface - Google Patents

A kind of method for preparing anticoagulation zinc-oxide film on copper surface Download PDF

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CN105970191B
CN105970191B CN201610321992.7A CN201610321992A CN105970191B CN 105970191 B CN105970191 B CN 105970191B CN 201610321992 A CN201610321992 A CN 201610321992A CN 105970191 B CN105970191 B CN 105970191B
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zinc
oxide film
solution
copper sheet
sample
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CN105970191A (en
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彭思博
薛名山
王法军
欧军飞
李长全
李文
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Nanchang Hangkong University
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    • C—CHEMISTRY; METALLURGY
    • C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
    • C23C—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
    • C23C18/00—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating
    • C23C18/02—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by thermal decomposition
    • C23C18/12—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by thermal decomposition characterised by the deposition of inorganic material other than metallic material
    • C23C18/1204—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by thermal decomposition characterised by the deposition of inorganic material other than metallic material inorganic material, e.g. non-oxide and non-metallic such as sulfides, nitrides based compounds
    • C23C18/1208—Oxides, e.g. ceramics
    • C23C18/1216—Metal oxides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L33/00—Antithrombogenic treatment of surgical articles, e.g. sutures, catheters, prostheses, or of articles for the manipulation or conditioning of blood; Materials for such treatment
    • A61L33/0005—Use of materials characterised by their function or physical properties
    • A61L33/0011—Anticoagulant, e.g. heparin, platelet aggregation inhibitor, fibrinolytic agent, other than enzymes, attached to the substrate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L33/00—Antithrombogenic treatment of surgical articles, e.g. sutures, catheters, prostheses, or of articles for the manipulation or conditioning of blood; Materials for such treatment
    • A61L33/18—Use of ingredients of undetermined constitution or reaction products thereof
    • C—CHEMISTRY; METALLURGY
    • C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
    • C23C—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
    • C23C18/00—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating
    • C23C18/02—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by thermal decomposition
    • C23C18/12—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by thermal decomposition characterised by the deposition of inorganic material other than metallic material
    • C23C18/1229—Composition of the substrate
    • C23C18/1241—Metallic substrates
    • C—CHEMISTRY; METALLURGY
    • C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
    • C23C—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
    • C23C18/00—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating
    • C23C18/02—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by thermal decomposition
    • C23C18/12—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by thermal decomposition characterised by the deposition of inorganic material other than metallic material
    • C23C18/125—Process of deposition of the inorganic material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents

Abstract

The invention discloses a kind of method for preparing anticoagulation zinc-oxide film on copper sheet surface, by preparing the Nano zinc oxide film with ultra-hydrophobicity on copper sheet surface, blood platelet can be effectively reduced in the adhesion amount of film surface and copper sheet is showed good anticoagulation function.The present invention prepares zinc-oxide film using electro-deposition with the technique that hydro-thermal method is combined, copper sheet is fixed on to one end of electrode, insert precursor solution and carry out electro-deposition, zinc oxide seed layer is prepared on copper sheet surface, hydro-thermal method is used again, certain temperature, time are controlled, after sintering at a certain temperature, tests its anticoagulation function.Production cost of the present invention is low, and preparation method is simple, requires low to consersion unit, reaction condition is gentle, non-environmental-pollution, can be mass-produced.

Description

A kind of method for preparing anticoagulation zinc-oxide film on copper surface
Technical field
The present invention relates to a kind of method for preparing anticoagulation zinc-oxide film on copper surface.
Background technology
Biomaterial is a kind of reparation that can be used for animal organ and tissue and replacement, the Clinics and Practices of disease, with moving Thing bio-compatible, the material with property or function.Anticoagulant biomaterial is the important component of biomaterial, quilt Be widely used in on human blood and the medical material that is in contact of tissue, such as hemodialysis system, extracorporeal circulation system, artificial Cardiac valves, pacemaker, artificial blood vessel, intravascular stent, surgical cable and conduit etc..With coronary artery and peripheral painstaking effort Atherosclerotic cardiovascular disease based on pipe disease, it is one of death rate highest disease in the world.2010, only in U.S. State, the expense for angiocardiopathy are just up to 448,500,000,000 dollars.The market of anticoagulant material is very huge, and with annual 10% -20% speed increases.Therefore the anticoagulant property of research zinc oxide makes great sense.
The content of the invention
In order to study the anticoagulation function on zinc-oxide film surface, anti-freezing is prepared on copper sheet surface the invention provides one kind The method of blood zinc-oxide film.
The technical solution used in the present invention is：A kind of method for preparing anticoagulation zinc-oxide film on copper sheet surface, its It is characterised by：By preparing the zinc nitrate solution of certain concentration, the copper sheet cleaned with constant voltage dc source to polishing carries out electricity Deposition, prepare Seed Layer, sample is put into reactor, pour into equimolar than Zn (NO3）2·6H2O and NH3 .H2O mixing Solution, take out, calcined after reacting a period of time under given conditions, then measure its surface static contact angle, choose super thin Water/super hydrophilic sample is put into platelet solution and centrifuged, and makes platelet adhesion reaction on film, is observed under ESEM.
The present invention to prepare feature process step as follows：
First, compound concentration is 0.01 mol/L to 0.05 mol/L Zn (NO3）2·6H2O solution, clean copper sheet is consolidated One end of electrode is scheduled on, electro-deposition, sedimentation time 10 are carried out in 50 DEG C to 90 DEG C of electrodeposit liquid with 2 V dc source min；
2nd, configuration concentration is 0.02 mol/L to 0.08 mol/L Zn (NO3）2·6H2O solution, instilled into solution etc. The NH of mol ratio3 .H2O, stir；
3rd, the copper sheet for having Seed Layer is put into reactor, then the solution that step 2 has been configured is poured into reactor, so The reactor of good seal is inserted in insulating box afterwards.2 h are reacted in 70 DEG C to 90 DEG C of atmospheric atmosphere to 4 h；
4th, reactor is taken out after it is down to room temperature, and sample is taken out, Muffle furnace is put into after being washed with deionized.Will Temperature setting is 200 DEG C, and soaking time is 2 h；
5th, the static contact angle that sample measures surface of nanometer zinc oxide after its cooling with contact angle measurement is taken out；
6th, the fresh blood of human body of 5 mL is taken, is shaken up, is centrifuged（3650 r/min）10 min.Draw whole supernatants extremely Another centrifuge tube, is centrifuged again（3000 r/min）10 min.The 3/4 of abandoning supernatant, remaining liq are high concentration blood platelet Blood plasma；
7th, take the phosphate buffer of 20 uL high concentration thrombocyte plasmas PH=7.4 to dilute 20 times, it is molten to obtain blood platelet Liquid；
8th, sample is immersed in platelet solution, then centrifuges 1 min with 3500 r/min rotating speeds, use phosphate Buffer solution rinses, and 2.0% glutaraldehyde fixes 1 h, then is dehydrated by ethanol series, acetone dealcoholysis；
9th, situations such as platelet PLA2, accumulation, deformation are observed under ESEM, to each sample, randomly selects 3 Taken pictures in individual equally distributed region.Observe and hematoblastic stick quantity and hematoblastic form.
Further, Seed Layer is prepared by electro-deposition in the step 1.
Further, NH is added in the step 23 .H2O amount is that solution is changed into clarification by muddiness just.
Further, reactor cools down in the step 4, with a large amount of cold water fast coolings, takes out sample and is placed on 200 DEG C At a temperature of, carry out in atmosphere, do not contain other protective gas.
Further, prepared in the step 5 with super hydrophilic or super-hydrophobic sample.
Further, centrifugal blood is obtained into high concentration thrombocyte plasma in the step 6.
Further, platelet adhesion reaction on copper sheet, protects its microscopic pattern in the step 8.
Further, hematoblastic form is observed in the step 9, counts hematoblastic quantity.
It is an advantage of the invention that：(1) preparation of Seed Layer so that the adhesion between zinc oxide and copper sheet is fine, overcomes The zinc oxide of generally existing holds flaky problem；(2) zinc oxide is prepared on copper sheet surface with hydro-thermal method, zinc oxide distribution is equal It is even, Stability Analysis of Structures；(3) zinc oxide is a kind of wide bandgap semiconductor materials, and has good biocompatibility, its hydrophobic table Face causes device to be not easy to pollute, sterilization easy to clean, is very promising anticoagulant material.(4) nitric acid that the present invention uses Zinc, ammoniacal liquor etc. are all conventional raw materials, and production cost is low, require low to consersion unit, and reaction condition is gentle, and preparation method is simple, Non-environmental-pollution.
Brief description of the drawings
Fig. 1 is the field emission scanning electron microscope figure of zinc-oxide film of the present invention；
Fig. 2 is SEM figure of the platelet adhesion reaction of the present invention on zinc-oxide film；
Fig. 3 is schematic diagram of the platelet adhesion reaction quantity of the present invention with contacting angular dependence.
Embodiment
Provide embodiments of the invention and combine the embodiment provided and the present invention is illustrated, but given embodiment The present invention is not limited in any way：
Embodiment one：Compound concentration is 0.01 mol/L Zn (NO3）2·6H2O solution, clean copper sheet is consolidated One end of electrode is scheduled on, electro-deposition is carried out in 50 DEG C of electrodeposit liquid with 2 V dc source, sedimentation time is 10 min； Configuration concentration is 0.05 mol/L Zn (NO3）2·6H2O solution, into solution instill equimolar than NH3 .H2O, it is sufficiently stirred Uniformly；The copper sheet for having Seed Layer is put into reactor, then the solution that step 2 has been configured is poured into reactor, then close The reactor sealed is inserted in insulating box.4 h are reacted in 90 DEG C of atmospheric atmosphere；Reactor is taken out after it is down to room temperature, Sample is taken out, Muffle furnace is put into after being washed with deionized.It it is 200 DEG C by temperature setting, soaking time is 2 h；
The static contact angle that sample measures surface of nanometer zinc oxide after its cooling with contact angle measurement is taken out, measures this The static contact angle of sample surfaces is 167.7 °, and roll angle is 6.7 °；The fresh blood of human body of 5 mL is taken, is shaken up, is centrifuged（3650 r/min）10 min.Whole supernatants are drawn to another centrifuge tube, are centrifuged again（3000 r/min）10 min.Abandoning supernatant 3/4, remaining liq is high concentration thrombocyte plasma；Take the phosphate-buffered of 20 uL high concentration thrombocyte plasmas PH=7.4 Liquid dilutes 20 times, obtains platelet solution；Sample is immersed in platelet solution, then centrifuges 1 with 3500 r/min rotating speeds Min, rinsed with phosphate buffer, 2.0% glutaraldehyde fixes 1 h, then is dehydrated by ethanol series, acetone dealcoholysis.
Situations such as platelet PLA2, accumulation, deformation are observed under ESEM, to each sample, randomly select 3 uniformly Taken pictures in the region of distribution.Observe it is hematoblastic stick quantity and hematoblastic form, blood platelet is estimated by SEM pictures Stick quantity for 0.74 × 104Individual/cm2Hematoblastic form does not also change, illustrates that blood platelet is not activated, the material Material has good anticoagulant functions.
Embodiment two：Compound concentration is 0.01 mol/L Zn (NO3）2·6H2O solution, clean copper sheet is consolidated One end of electrode is scheduled on, electro-deposition is carried out in 90 DEG C of electrodeposit liquid with 2 V dc source, sedimentation time is 10 min； Configuration concentration is 0.05 mol/L Zn (NO3）2·6H2O solution, into solution instill equimolar than NH3 .H2O, it is sufficiently stirred Uniformly；The copper sheet for having Seed Layer is put into reactor, then the solution that step 2 has been configured is poured into reactor, then close The reactor sealed is inserted in insulating box.4 h are reacted in 90 DEG C of atmospheric atmosphere；Reactor is taken out after it is down to room temperature, Sample is taken out, Muffle furnace is put into after being washed with deionized.It it is 200 DEG C by temperature setting, soaking time is 2 h；
The static contact angle that sample measures surface of nanometer zinc oxide after its cooling with contact angle measurement is taken out, measures this The static contact angle of sample surfaces is 3.7 °；The fresh blood of human body of 5 mL is taken, is shaken up, is centrifuged（3650 r/min）10 min. Whole supernatants are drawn to another centrifuge tube, are centrifuged again（3000 r/min）10 min.The 3/4 of abandoning supernatant, raffinate Body is high concentration thrombocyte plasma；Take the phosphate buffer of 20 uL high concentration thrombocyte plasmas PH=7.4 to dilute 20 times, obtain To platelet solution；Sample is immersed in platelet solution, then 1 min is centrifuged with 3500 r/min rotating speeds, uses phosphate Buffer solution rinses, and 2.0% glutaraldehyde fixes 1h, then is dehydrated by ethanol series, acetone dealcoholysis.
Situations such as platelet PLA2, accumulation, deformation are observed under ESEM, to each sample, randomly select 3 Taken pictures in the region of even distribution.Observe it is hematoblastic stick quantity and hematoblastic form, by SEM pictures estimate bleeding it is small Plate sticks quantity for 1.36 × 104Individual/cm2Hematoblastic form does not also change, illustrates that blood platelet is not activated, should The anticoagulant functions of material are general.
Embodiment three：Compound concentration is 0.05 mol/L Zn (NO3）2·6H2O solution, clean copper sheet is consolidated One end of electrode is scheduled on, electro-deposition is carried out in 70 DEG C of electrodeposit liquid with 2 V dc source, sedimentation time is 10 min； Configuration concentration is 0.05 mol/L Zn (NO3）2·6H2O solution, into solution instill equimolar than NH3 .H2O, it is sufficiently stirred Uniformly；The copper sheet for having Seed Layer is put into reactor, then the solution that step 2 has been configured is poured into reactor, then close The reactor sealed is inserted in insulating box.4 h are reacted in 90 DEG C of atmospheric atmosphere；Reactor is taken out after it is down to room temperature, Sample is taken out, Muffle furnace is put into after being washed with deionized.It it is 200 DEG C by temperature setting, soaking time is 2 h；
The static contact angle that sample measures surface of nanometer zinc oxide after its cooling with contact angle measurement is taken out, measures this The static contact angle of sample surfaces is 4.8 °；The fresh blood of human body of 5 mL is taken, is shaken up, is centrifuged（3650 r/min）10 min. Whole supernatants are drawn to another centrifuge tube, are centrifuged again（3000 r/min）10 min.The 3/4 of abandoning supernatant, raffinate Body is high concentration thrombocyte plasma；Take the phosphate buffer of 20 uL high concentration thrombocyte plasmas PH=7.4 to dilute 20 times, obtain To platelet solution；Sample is immersed in platelet solution, then 1 min is centrifuged with 3500 r/min rotating speeds, uses phosphorus Phthalate buffer rinses, and 2.0% glutaraldehyde fixes 1 h, then is dehydrated by ethanol series, acetone dealcoholysis.
Situations such as platelet PLA2, accumulation, deformation are observed under ESEM, to each sample, randomly select 3 Taken pictures in the region of even distribution.Observe it is hematoblastic stick quantity and hematoblastic form, by SEM pictures estimate bleeding it is small Plate sticks quantity for 1.48 × 104Individual/cm2Hematoblastic form does not also change, illustrates that blood platelet is not activated, should The anticoagulant functions of material are general.
Embodiment four：Compound concentration is 0.01 mol/L Zn (NO3）2·6H2O solution, clean copper sheet is consolidated One end of electrode is scheduled on, electro-deposition is carried out in 70 DEG C of electrodeposit liquid with 2 V dc source, sedimentation time is 10 min； Configuration concentration is 0.05 mol/L Zn (NO3）2·6H2O solution, into solution instill equimolar than NH3 .H2O, it is sufficiently stirred Uniformly；The copper sheet for having Seed Layer is put into reactor, then the solution that step 2 has been configured is poured into reactor, then close The reactor sealed is inserted in insulating box.4 h are reacted in 70 DEG C of atmospheric atmosphere；Reactor is taken out after it is down to room temperature, Sample is taken out, Muffle furnace is put into after being washed with deionized.It it is 200 DEG C by temperature setting, soaking time is 2 h；
The static contact angle that sample measures surface of nanometer zinc oxide after its cooling with contact angle measurement is taken out, measures this The static contact angle of sample surfaces is 25.4 °；The fresh blood of human body of 5 mL is taken, is shaken up, is centrifuged（3650 r/min）10 min. Whole supernatants are drawn to another centrifuge tube, are centrifuged again（3000 r/min）10 min.The 3/4 of abandoning supernatant, raffinate Body is high concentration thrombocyte plasma；Take the phosphate buffer of 20 uL high concentration thrombocyte plasmas PH=7.4 to dilute 20 times, obtain To platelet solution；Sample is immersed in platelet solution, then 1 min is centrifuged with 3500 r/min rotating speeds, uses phosphorus Phthalate buffer rinses, and 2.0% glutaraldehyde fixes 1 h, then is dehydrated by ethanol series, acetone dealcoholysis.
Situations such as platelet PLA2, accumulation, deformation are observed under ESEM, to each sample, randomly select 3 Taken pictures in the region of even distribution.Observe it is hematoblastic stick quantity and hematoblastic form, by SEM pictures estimate bleeding it is small Plate sticks quantity for 2.03 × 104Individual/cm2Hematoblastic form does not also change, illustrates that blood platelet is not activated, should The anticoagulant functions of material are general.

Claims (7)

  1. A kind of 1. method for preparing anticoagulation zinc-oxide film on copper surface, it is characterised in that：Prepared and provided by electrodeposition process There are specific array density and the zinc oxide seed layer of thickness, then prepared with hydro-thermal method with the super-hydrophobic of specific microscopic appearance Zinc-oxide film, it was demonstrated that super-hydrophobic zinc-oxide film has anticoagulant property, carries out according to the following steps：
    First, compound concentration is 0.01 mol/L to 0.05 mol/L Zn (NO3)2·6H2O solution, clean copper sheet is fixed on One end of electrode, electro-deposition, sedimentation time 10 are carried out in 50 DEG C to 90 DEG C of electrodeposit liquid with 2 V dc source min；
    2nd, configuration concentration is 0.02 mol/L to 0.08 mol/L Zn (NO3）2·6H2O solution, equimolar is instilled into solution The NH of ratio3 .H2O, stir；
    3rd, the copper sheet for having Seed Layer is put into reactor, then the solution that step 2 has been configured is poured into reactor, then The reactor of good seal is inserted in insulating box；2 h are reacted under conditions of 70 DEG C to 90 DEG C to 4 h；
    4th, reactor is taken out after it is down to room temperature, and sample is taken out, Muffle furnace is put into after being washed with deionized；By temperature 200 DEG C are arranged to, soaking time is 2 h；
    5th, the static contact angle that sample measures surface of nanometer zinc oxide after its cooling with contact angle measurement is taken out；
    6th, the fresh blood of human body of 5 mL is taken, is shaken up, new blood is centrifuged into 10 min with 3650 r/min rotating speeds；
    Whole supernatants are drawn to another centrifuge tube, supernatant is centrifuged into 10 min with 3000 r/min rotating speeds again；Discard The 3/4 of clear liquid, remaining liq are high concentration thrombocyte plasma；
    7th, take the phosphate buffer of 20 μ L high concentration thrombocyte plasmas pH=7.4 to dilute 20 times, obtain platelet solution；
    8th, sample is immersed in platelet solution, then centrifuges 1 min with 3500 r/min rotating speeds, use phosphate buffer Rinsing, 1 h is fixed with the glutaraldehyde that mass percent concentration is 2.0%, then be dehydrated by ethanol series, acetone dealcoholysis；
    9th, situations such as platelet PLA2, accumulation, deformation are observed under ESEM, to each sample, 3 are randomly selected uniformly The region of distribution is taken pictures, it is found that hematoblastic quantity of sticking is seldom, shown obvious anticoagulant property.
  2. A kind of 2. method for preparing anticoagulation zinc-oxide film on copper surface according to claim 1, it is characterised in that：Institute State in step 1 and Seed Layer is prepared by electro-deposition.
  3. A kind of 3. method for preparing anticoagulation zinc-oxide film on copper surface according to claim 1, it is characterised in that：Institute State and NH is added in step 23 .H2O amount is that solution is changed into clarification by muddiness just.
  4. A kind of 4. method for preparing anticoagulation zinc-oxide film on copper surface according to claim 1, it is characterised in that：Institute State reactor in step 4 to cool down, with a large amount of cold water fast coolings, take out sample and be placed at a temperature of 200 DEG C, enter in atmosphere OK, other protective gas are not contained.
  5. A kind of 5. method for preparing anticoagulation zinc-oxide film on copper surface according to claim 1, it is characterised in that：Institute State and prepared in step 5 with super-hydrophobic zinc-oxide film.
  6. A kind of 6. method for preparing anticoagulation zinc-oxide film on copper surface according to claim 1, it is characterised in that：Institute State centrifugal blood in step 6, obtain high concentration thrombocyte plasma.
  7. A kind of 7. method for preparing anticoagulation zinc-oxide film on copper surface according to claim 1, it is characterised in that：Institute It is seldom to state quantity of the platelet adhesion reaction on copper sheet in step 8.
CN201610321992.7A 2016-05-16 2016-05-16 A kind of method for preparing anticoagulation zinc-oxide film on copper surface Expired - Fee Related CN105970191B (en)

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CN103157590A (en) * 2013-03-14 2013-06-19 许昌学院 Super-hydrophobic surface based on zinc and preparation method thereof
CN104357827A (en) * 2014-11-03 2015-02-18 中国石油大学(华东) Preparation method for super-hydrophobic corrosion-resistant copper-based surface
CN104674197A (en) * 2015-01-31 2015-06-03 南昌航空大学 Method for preparing ice-coating resistant zinc oxide coating on copper surface
CN105463564A (en) * 2015-11-30 2016-04-06 东南大学 ZnO nanorod and ZnO cluster composite structure and preparation method thereof

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