CN105954512A - Detection strip for Zika virus detection by means of fast immunochromatography method - Google Patents
Detection strip for Zika virus detection by means of fast immunochromatography method Download PDFInfo
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- CN105954512A CN105954512A CN201610384076.8A CN201610384076A CN105954512A CN 105954512 A CN105954512 A CN 105954512A CN 201610384076 A CN201610384076 A CN 201610384076A CN 105954512 A CN105954512 A CN 105954512A
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- Prior art keywords
- district
- detection
- virus
- antibody
- detector bar
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
Abstract
The invention provides a detection strip or kit for Zika virus detection by means of the fast immunochromatography method. The immunodetection strip has six areas. The first area is a sample adding pad; the second area is an immobilized Zika antigen marker area or anti-Zika virus antigen specific antibody marker; the third area is a detection area T and a coated antibody spray area, and the area T is matched with the second area; the fourth area is a contrast area used for immobilization of non-specific antibodies; the fifth area is a water absorber; the sixth area is a nitro fiber chromatography film. According to the detection strip or kit, only a small number of samples to be tested are needed, no equipment is required, a detection result can be obtained within ten minutes, detection is easy and fast, and the detection strip or kit can be purchased from a pharmacy so that patients can conduct detection by themselves.
Description
Technical field
The present invention relates to field of immunodetection, particularly relate to a kind of fast immune chromatographic method detection fork clip virus
Detector bar or detection box.
Background technology
In May, 2015 rises, and fork clip virus (Zika virus) epidemic situation is in Brazil, and South America and Latin America are big
The most popular, and oriented whole world diffusion tendency, existing more than 150 ten thousand people of Brazil infect so far.Infection symptoms
The symptoms such as gentle such as fever, erythra, arthralgia or conjunctivitis, and microcephalus baby can be caused with big
Brain injury, also can cause adult neural to damage, even dead.
Fork clip virus (Zika virus) is propagated by mosquito bite, also can be propagated by Placenta Hominis, current city
On Chang, specific Therapeutic Method be there is no for fork clip virus, for the detection of this virus, mostly use PCR
Method, but this method need distinctive PCR instrument, in addition it is also necessary to the operation that professional and technical personnel is fine, and
And expensive, detection is the longest.Therefore, once state of an illness outburst or popular, can be fast and convenient
Detect this virus and become the key factor that can effectively control epidemic situation development.
Summary of the invention
In view of this, it is an object of the invention to provide a kind of fast immune chromatographic method detection fork clip virus
Detector bar or detection box.
In order to realize foregoing invention purpose, the present invention provides techniques below scheme:
The invention provides detector bar or the detection of a kind of fast immune chromatographic detection fork clip virus Zika virus
Box, described immunoassay strip is divided into 6 districts: the firstth district is sample-adding pad；Secondth district is that immobilization fork clip resists
Former label district or the specific antibody label of anti-fork clip virus antigen；3rd district is that detection zone T is for being coated
Antibody spraying area, detection zone T and the secondth district match；4th district is check plot, and immobilization is non-specific anti-
Body；5th district is water aspirator；6th district is nitrocellulose chromatographic film；
Add and drip after testing sample, by or do not make testing liquid up along chromatographic film, at T by buffer
District forms immune complex, and its intensity is directly proportional to determinand content.As T district occurs that colour band is that fork clip is sick
Poison infects positive findings.As T district does not has colour band to occur, then do not infected by fork clip virus for object to be measured.
Preferably, at detector bar detection zone T district spraying human body immunoglobulin IgG or IgM, second
District's label is fork clip Viral extract or fork clip viral gene albumen, when testing result, such as virus sense
There is " human body immunoglobulin IgG or IgM antiviral antibody virus signature thing " in dye detection zone T district
Immune complex precipitation line, testing sample containing human body through fork clip virus infect thus produce antibody.
Preferably, in detector bar detection zone T district, spraying is coated specificity animal anti-fork clip antiviral antibody, the
Two district's labels are another specificity animal anti-fork clip antiviral antibody matched with T district coated antibody,
During testing result, as virus infects, detection zone T district occurs " being coated antiviral antibody virus antigen to be measured
Labeled virus antibody " immune complex precipitation line, testing sample virus antigen Han fork clip.
Preferably, described detector bar is placed in a detection box.
Preferably, testing sample is in vitro blood sample, serum, blood plasma, urine, nose liquid or finger blood.
Preferably, after testing sample adds the firstth district, available buffer testing sample is launched up or
Without buffer, directly launch with sample.
Beneficial effects of the present invention: detector bar of the present invention or detection box, it is possible to the most special inspection
Survey fork clip virus, the most only needs a small amount of testing sample, within ten minutes, can obtain testing result,
Need not by any equipment, and pharmacy purchase can be gone up, but patient self detection.
Detailed description of the invention
The invention provides the detector bar of a kind of fast immune chromatographic detection fork clip virus Zika virus, described
Immunoassay strip is divided into 6 districts: the firstth district is sample-adding pad；Secondth district is immobilization fork clip antigenic label
District or the specific antibody label of anti-fork clip virus antigen；3rd district be detection zone T be coated antibody spraying
District, detection zone T and the secondth district match；4th district is check plot, immobilization nonspecific antibody；5th
District is water aspirator；6th district is nitrocellulose chromatographic film.
The detection of the detector bar of fast immune chromatographic of the present invention detection fork clip virus Zika virus antibody
The reaction principle of antiviral antibody and process: dropping testing sample rises according to chromatographic theory in sample-adding pad, liquid,
There is antiviral antibody as in sample, walk to the secondth district, form " antibody-marker antigen " again with traget antibody
Compound.Described complex persistently rises to detection zone T, forms " insolubilized antibody-anti-at detection zone T
Antiviral antibody-labelled antigen " complex, developed band is existing in detection zone T, and its result is antiviral antibody detection
Being positive, as detection zone T manifests without colour band, its result infects, for virus, the result that is negative.
The detection of the detector bar of fast immune chromatographic of the present invention detection fork clip virus Zika virus antigen
The reaction principle of virus antigen and process: dropping testing sample is in sample-adding pad；Liquid is according to chromatography spreading principle
Up.As there is antigen in sample, forming " antigen-labelling is anti-" complex, this complex is persistently gone up
Rise to detection zone T, at detection zone T, form " solid matrix antibody-antigen-traget antibody " complex.As for
Gold label test strip, developed band is revealed in detection zone T, and its result is that virus is positive in Detection of antigen.Such as detection
District T manifests without colour band, and its result is that virus is negative in Detection of antigen.
Fast immune chromatographic of the present invention detects the concrete of the detector bar of fork clip virus Zika virus antibody
Structure preferably as it is shown in figure 1, be arranged in order from the bottom of detector bar the firstth district, the secondth district, the 6th district,
3rd district, the 4th district, the 5th district.Wherein the firstth district is positioned at the bottom of detector bar, for sample-adding pad, uses
During testing sample added drip in the sample pad in the firstth district；Secondth district is close to the firstth district, for labelling
Virus antigen solid phase small pieces, the label on the solid phase small pieces in the secondth district is fork clip Viral extract or fork clip
Viral gene albumen；6th district is positioned in the middle of the secondth district and the 3rd district, for nitrocellulose chromatographic film district,
It is fixed with celluloid chromatographic film the 3rd district on it and is positioned in the middle of the 6th district and the 4th district, for detection zone T,
Coated antibody spraying zone, the most preferably includes that T1 detection line and T2 detect line, described T1 inspection
Fixing exempting from for animal is anti-human for fix on animal human immunoglobulins IgG, T2 detection line on survey line
Epidemic disease globulin IgM；4th district is positioned in the middle of the 3rd district and the 5th district, for check plot, in check plot solid phase
Change nonspecific antibody；5th district is suction zones, and the 5th district is fixed with paper fibre plate, is used for absorbing moisture.
Heretofore described human body immunoglobulin IgG, IgM and fork clip Viral extract or fork clip are sick
Virus gene albumen is preferably from U.S. JAJ international, Inc.San Diego, in manufacture method reference
State patent ZL 00118669.8, ZL98122892.5, ZL03142460.0, wherein said fork clip virus
Extract or the preferred material of fork clip viral gene albumen are from U.S. JAJ international, Inc.San
Diego。
Fast immune chromatographic of the present invention detects the concrete of the detector bar of fork clip virus Zika virus antigen
Structure preferably as in figure 2 it is shown, be arranged in order from the bottom of detector bar the firstth district, the secondth district, the 6th district,
3rd district, the 4th district, the 5th district.Wherein the firstth district is positioned at the bottom of detector bar, for sample-adding pad, uses
During testing sample added drip in the sample pad in the firstth district；Secondth district is close to the firstth district, for solid phase
Traget antibody, preferably gold mark monoclonal antibody；6th district is positioned in the middle of the secondth district and the 3rd district, for nitre
Base fiber chromatographic film district, the 3rd district is positioned in the middle of the 6th district and the 4th district, sprays for detection zone T: coated antibody
Being coated with zone, detection zone T coated antibody and second district's traget antibody match. and the 4th district is positioned at the 3rd district and the
In the middle of 5th district, for check plot, at check plot immobilization nonspecific antibody；5th district is suction zones, the 5th
It is fixed with paper fibre plate in district, is used for absorbing moisture.
The fast immune chromatographic provided the present invention below in conjunction with embodiment detects fork clip virus Zika virus's
Detector bar is described in detail, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1
The detector bar of fast immune chromatographic detection fork clip virus Zika virus antibody or detection box, described immunity
Detector bar is divided into 6 districts: wherein the firstth district is positioned at one end of detector bar, pads for sample-adding, during use
Testing sample is added and drips in the sample pad in the firstth district；Secondth district is close to the firstth district, resists for labeled virus
Former solid phase small pieces, the label on the solid phase small pieces in the 3rd district be labelled antigen be gene protein numbering 1248；
6th district is positioned in the middle of the secondth district and the 3rd district, for nitrocellulose chromatographic film district, is fixed with nitric acid thereon
Cellulose chromatographic film；3rd district is positioned in the middle of the 6th district and the 4th district, and for detection zone T, coated antibody sprays
Zone, the most preferably includes that T1 detection line and T2 detect line, described T1 detection line is fixed
For fixing for animal human immunoglobulins IgM on animal human immunoglobulins IgG, T2 detection line；
T1 district spraying human immunoglobulins's IgG numbering 701-2 mg/ml, T2 district spraying anti-human immunity ball
Protein I gM numbering 704-2 mg/ml, the 4th district is positioned in the middle of the 3rd district and the 5th district, for check plot,
At check plot immobilization nonspecific antibody；5th district is suction zones, and the 5th district is fixed with paper fibre plate,
It is used for absorbing moisture.
By specification accompanying drawing Fig. 1 assembles chromatography strip, puts and gives in detection box, and during detection, dropping 1-2 drips expansion
Liquid, observed testing result in 10 minutes, and result is as shown in Figure of description Fig. 3, and wherein Fig. 3 A is T
District sprays the positive findings schematic diagram of IgG and IgM simultaneously, and Fig. 3 B is the positive knot of T district spraying IgG
Really schematic diagram, Fig. 3 C is the positive findings schematic diagram of spraying IgM, and Fig. 3 D is negative findings schematic diagram.
Embodiment 2
The detector bar of fast immune chromatographic detection fork clip virus Zika virus antigen, described immunoassay strip is altogether
Divide 6 districts: wherein the firstth district is positioned at one end of detector bar, for sample-adding pad, test sample during use, will be treated
Product add drip in the sample pad in the firstth district；Secondth district is close to the firstth district, little for labeled virus antibody solid phase
Sheet, the label on the solid phase small pieces in the 3rd district is coated antibody: T district spraying fork clip viral monoclonal antibodies
Numbering 084-2 mg/ml；6th district is positioned in the middle of the secondth district and the 3rd district, for nitrocellulose chromatographic film
District, is fixed with celluloid chromatographic film thereon；3rd district is positioned in the middle of the 6th district and the 4th district, for
Detection zone T, traget antibody is that gold marks another monoclonal antibody numbering 024-2 mg/ml matched,
4th district is positioned in the middle of the 3rd district and the 5th district, for check plot, at check plot immobilization nonspecific antibody；
5th district is suction zones, and the 5th district is fixed with paper fibre plate, is used for absorbing moisture.
By specification accompanying drawing Fig. 2 assembles chromatography strip, puts and gives in detection box, during detection, instills 1 finger
Blood blood sample is in first district's well, and 1 developping solution of dropping immediately after, in 10 minutes observation detection knots
Really, result is as shown in Figure of description Fig. 4, and wherein Fig. 4 A is positive findings schematic diagram, and Fig. 4 B is cloudy
Property result schematic diagram.
As seen from the above embodiment, detector bar of the present invention or detection box, it is possible to the most special inspection
Survey fork clip virus, the most only needs a small amount of testing sample, within ten minutes, can obtain testing result,
It is not required to by any equipment, and pharmacy purchase can be gone up, but patient self detection.
The above is only the preferred embodiment of the present invention, it is noted that general for the art
For logical technical staff, under the premise without departing from the principles of the invention, it is also possible to make some improvement and profit
Decorations, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (6)
1. the detector bar of a fast immune chromatographic detection fork clip virus Zika virus, it is characterised in that immunity
Detector bar is divided into 6 districts: the firstth district is sample-adding pad；Secondth district be immobilization fork clip antigenic label district or
The specific antibody label of anti-fork clip virus antigen；3rd district is detection zone T coated antibody spraying area, inspection
Survey district T and the secondth district matches；4th district is check plot, and it is non-specific that described check plot is provided with immobilization
Antibody；5th district is water aspirator；6th district is nitrocellulose chromatographic film；
2. according to the detector bar described in claim 1, it is characterised in that in detector bar detection zone T district, spraying is anti-
Human immunoglobulin IgG or IgM, second district's label is fork clip Viral extract or fork clip virus base
Because of albumen, when testing result, as virus infects, there is " human body immunoglobulin IgG in detection zone T district
Or IgM antiviral antibody virus signature thing " immune complex precipitation line, testing sample contains human body through fork clip
The viral antibody infected thus produce.
3. according to the detector bar described in claim 1, it is characterised in that at detector bar detection zone T district spraying bag
By specificity animal anti-fork clip antiviral antibody, second district's label is another with what T district coated antibody matched
One specificity animal anti-fork clip antiviral antibody, when testing result, as virus infects, detection zone T district goes out
Existing " being coated antiviral antibody virus antigen to be measured labeled virus antibody " immune complex precipitation line, to be measured
Sample virus antigen Han fork clip.
4. according to the detector bar described in claim 1, it is characterised in that described detector bar is placed in a detection
In box.
5. according to the detector bar described in claim 1, it is characterised in that described testing sample is in vitro blood,
Urine, nose liquid or finger blood.
6., according to the detector bar described in claim 1, it is characterised in that after testing sample adds the firstth district, use
Testing sample is launched up or without buffer by buffer, directly launches with sample.
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| CN201610384076.8A CN105954512A (en) | 2016-06-01 | 2016-06-01 | Detection strip for Zika virus detection by means of fast immunochromatography method |
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| CN201610384076.8A CN105954512A (en) | 2016-06-01 | 2016-06-01 | Detection strip for Zika virus detection by means of fast immunochromatography method |
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