CN105954331B - A kind of paper base electrode detection platform and preparation method thereof for biochemical analysis - Google Patents
A kind of paper base electrode detection platform and preparation method thereof for biochemical analysis Download PDFInfo
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- CN105954331B CN105954331B CN201610285084.7A CN201610285084A CN105954331B CN 105954331 B CN105954331 B CN 105954331B CN 201610285084 A CN201610285084 A CN 201610285084A CN 105954331 B CN105954331 B CN 105954331B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/308—Electrodes, e.g. test electrodes; Half-cells at least partially made of carbon
Abstract
The paper base electrode detection platform and preparation method thereof that the invention discloses a kind of for biochemical analysis, the paper base electrode detection platform for biochemical analysis, using degradable paper wood as base material, carbon electrode is printed on base material, in the fixed electron transfer mediator of carbon electrodes and identification molecular layer, the completely new paper base electrode detection platform of Wholly-degradable is obtained.Prepared degradable paper base electrode detection platform shows there is same good chemical property with its plastic basis material electrode by electro-chemical test.Paper base electrode detection platform based on Wholly-degradable has many advantages, such as that high sensitivity, detection time are short, otherness is small, stability is good for detecting glucose.
Description
Technical field
The invention belongs to biosensor technique field more particularly to a kind of paper base electrode detection platforms for biochemical analysis
And preparation method thereof.
Background technique
Currently, disposable plastics base bioelectrode test piece is in fields such as medical diagnosis, food inspection and environment measurings
There is important application.Especially when medical diagnostic field is used to detect the blood glucose of diabetic, since its is portable, side
Just, the advantages that cheap, is widely used.According to investigation in 2008, just there are about the examinations of 6,000,000,000 plastics base blood glucose within 1 year in the whole world
Piece is consumed.According to the investigation statistics of International Diabetes Federation the results show that by 2013 global diabetes illness quantity
There are about 3.82 hundred million numbers.It is measured daily at least with blood glucose test according to every diabetic of suggestion of International Diabetes Federation
Four blood glucose.This generates a large amount of Biohazard Waste needs are processed.And these current discardable biosensors are all
Be based on plastic-substrates (such as PC, PVC and PET) and preparation, the main processing of the Biohazard Waste after a large amount of uses are main
It is to be embedded in underground or burning processing, plastic-substrates are embedded in underground and are difficult to be degraded after burning processing, can also generate a large amount of dirt
Contaminate object.Cellulose is a kind of organic matter of natural degradable, from a wealth of sources, rich reserves in nature, it is estimated that, by photosynthetic
Act on the cellulose synthesized every year to 1011~1012Ton.Other than from a wealth of sources, rich reserves, it is most important that, fiber
Plain or a kind of natural biodegradable, good biocompatibility, a kind of material of recyclable regenerative.Moreover, also having appropriate
Mechanical mechanics property, light weight is soft, easy to carry, is very suitable for preparing analysis and testing equipment.
However paper base analytical equipments all at present is all based on greatly the test piece of colour generation method preparation, based on electrochemical principle
The full paper base test piece of electrochemistry belongs to technological gap.
Summary of the invention
The paper base electrode detection platform and preparation method thereof that the purpose of the present invention is to provide a kind of for biochemical analysis, purport
It is all based on test piece prepared by colour generation method, the electrochemistry based on electrochemical principle greatly solving paper base analytical equipments all at present
The problem of full paper base test piece belongs to technological gap.
The invention is realized in this way a kind of preparation method of the paper base electrode detection platform for biochemical analysis, described
The preparation method of paper base electrode detection platform for biochemical analysis is printed on base material using degradable paper wood as base material
There is carbon electrode, fixed upper electron transfer mediator and identification molecule, obtain degradable paper base electrode in the working region of carbon electrode
Detection platform.
Further, carbon electrode is prepared by screen printing technique, and moves into baking oven and is solidified, obtain paper base material carbon electricity
Pole.
Further, the solution of 20 ~ 50 μ L is added dropwise in carbon electricity in the potassium ferricyanide solution that compound concentration is 50 mMs every liter
Pole surface, and be placed in 25 DEG C ~ 30 DEG C of baking oven, it saves 10 ~ 30 minutes.
It further, is the glucose oxidase solution of 10 K international units by activity, supplying, which arrives, contains 0.05 gram per liter
In 50 mMs every liter of phosphate buffer of sodium carboxymethylcellulose, the solution of 20 ~ 50 μ L is added dropwise in carbon electrodes, and
It is placed in 25 DEG C ~ 30 DEG C of baking oven, saves 10 ~ 30 minutes, prepare paper base electrode test piece.
Paper base electrode detection platform of the present invention for biochemical analysis, is made of four parts from top to down, is respectively as follows:
Identify molecular layer, electron transfer mediator layer, carbon electrode, paper base material；
It is printed with carbon electrode in the paper base material substrate, carbon electrode is prepared by screen printing technique；Carbon electrode
One end is placed with electron transfer mediator layer, and electron transfer mediator is to be fixed on carbon electrodes, mesh by the effect of physical absorption
Be for transmit identification molecule and electrode between electronics transfer；It is placed with identification molecular layer on electron transfer mediator layer, knows
Other molecule is fixed on carbon electrodes also with the effect of physical absorption, for reacting with test substance.
Further, the material of the paper base material is cellulose paper.
Further, the paper base electrode detection platform is the pectination finger-fork type carbon electrode institute structure on the base material
At.
Further, the electron transfer mediator layer is the potassium ferricyanide, 1,4-benzoquinone, methylene blue or methyl ferrocene.
Further, the identification molecular layer is enzyme, antibody, DNA, aptamers or cell.
Another object of the present invention is to preparing one kind has green, environmental protection, and biodegradable paper base electrode detection is flat
Platform, the preparation method comprising the paper base electrode detection platform for biochemical analysis.
Provided by the present invention for the paper base electrode detection platform and preparation method thereof of biochemical analysis, using paper wood as substrate
Material prepares carbon electrode, and fixed suitable identification molecule by screen printing technique, prepares the completely new paper of Wholly-degradable
Base electrode detection platform；The paper base electrode detection platform of prepared Wholly-degradable is shown and its plastics by electro-chemical test
Electrode of the substrate has same good chemical property.The electrochemistry paper base test piece for developing Wholly-degradable, it is current for solving
The processing problem of the waste of commercial plastics base electrode test piece has great importance, to solve the useless of plastics base test strip
Gurry processing solves to provide new idea and method.The present invention is base material using paper wood, passes through screen printing technique energy
Enough prepare the paper base Electrochemical Detection platform of the Wholly-degradable with excellent electrochemical performance；Based on degradable paper base electricity
Chemical detection platform, the fixed suitable potassium ferricyanide and glucose oxidase prepare completely new glucose paper base test piece, and can be quasi-
True is used to detect the glucose solution within the scope of a certain concentration, and the paper base blood glucose test for preparing Wholly-degradable has weight
The meaning wanted also provides new idea and method to solve the waste disposal issues of plastics base blood glucose test.The present invention uses
When detecting glucose solution, there are high sensitivity (2.07 μ A cm-2), detection time is short (less than 40 s), the small (phase of otherness
To standard deviation less than 4.26 %, N=5) and stability it is good (be stored in 4 DEG C of environment, still have after 31 days 93.9% just
The response of beginning electric current) the advantages that；Full utilization paper wood exploitation prepares a kind of paper base electrode detection platform of Wholly-degradable, to solution
Certainly largely had great importance at present using the processing problem of waste caused by plastics base electrode test piece difficult to degrade.This
Inventive method preparation process is simple, at low cost, to solve the problems, such as that the waste pollution of plastics base electrode test piece difficult to degrade provides
New method and solution route.Of the invention is used to detect glucose based on paper base electrode detection platform, in a certain concentration model
Good linear relationship is presented in its response current and concentration of glucose in enclosing, and has high sensitivity, and detection time is short, otherness
The advantages that small and stability is good；Present invention demonstrates that paper base electrode detection platform can replace current plastics base test strip, it is
The offal treatment solution for solving plastics base test strip provides new method.
Detailed description of the invention
Fig. 1 is the preparation method process of the paper base electrode detection platform provided in an embodiment of the present invention for biochemical analysis
Figure.
Fig. 2 is the paper base electrode detection platform structure schematic diagram provided in an embodiment of the present invention for biochemical analysis；
In figure: 1, identifying molecular layer；2, electron transfer mediator layer；3, carbon electrode；4, paper base material.
Fig. 3 is provided in an embodiment of the present invention for detecting the range of linearity and sensitivity schematic diagram of glucose.
Fig. 4 is the Clarke error analysis that paper base electrode detection platform provided in an embodiment of the present invention is used to detect glucose
Figure.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Application principle of the invention is explained in detail with reference to the accompanying drawing.
As shown in Figure 1, the preparation method of the paper base electrode detection platform for biochemical analysis of the embodiment of the present invention includes
Following steps:
S101: using the paper wood of Wholly-degradable as base material, carbon electrode is prepared by screen printing technique, and move into baking
Solidified in case, obtains completely new paper base material carbon electrode；
S102: electron transfer mediator solution is prepared, and is added dropwise and is fixed in carbon electrodes；
S103: by identification molecule supplying into phosphate buffer, and being added dropwise the working region for being fixed on electrode surface,
Prepare the completely new paper base electrode detection platform of Wholly-degradable.
As shown in Fig. 2, the paper base electrode detection platform for biochemical analysis of the embodiment of the present invention mainly by: identification molecule
Layer 1, electron transfer mediator layer 2, carbon electrode 3, paper base material 4 form.
Carbon electrode 3 is printed on paper base material 4, one end of carbon electrode 3 is placed with electron transfer mediator layer 2, and electron transmission is situated between
Identification molecular layer 1 is placed on matter layer 2.
Application principle of the invention is further described combined with specific embodiments below.
Embodiment 1:
(1) using the cellulose paper of Wholly-degradable as base material, carbon electrode is prepared by screen printing technique, and move into
Solidified in baking oven, obtains completely new paper base material carbon electrode；
(2) solution of 20 ~ 50 μ L is added dropwise in carbon electrode table in the potassium ferricyanide solution that compound concentration is 50 mMs every liter
Face, and be placed in 25 DEG C ~ 30 DEG C of baking oven, it saves 10 ~ 30 minutes；
It (3) is the glucose oxidase solution of 10 K international units, supplying to the carboxylic first containing 0.05 gram per liter by activity
In 50 mMs every liter of phosphate buffer of base sodium cellulosate, the solution of 20 ~ 50 μ L is added dropwise in carbon electrodes, and be placed on
It in 25 DEG C ~ 30 DEG C of baking oven, saves 10 ~ 30 minutes, prepares the completely new paper base electrode examination of the detection glucose of Wholly-degradable
Piece.
The schematic diagram of prepared completely new paper base electrode detection platform, as shown in Figure 2.
Application effect of the invention is explained in detail below with reference to experiment.
(1) by the paper base electrode test piece of the completely new detection glucose prepared, the glucose solution of various concentration is added dropwise
In the working region of paper base electrode test piece, by 400 electrochemical workstation of CHI using chronoamperometry detect response current with
Relationship between concentration of glucose, as shown in Figure 3.Its result illustrates sensitivity with higher (2.07 μ A cm-2), and it is complete
New paper base electrode test piece is when for detecting glucose with shorter detection time (less than 40 s) and lesser difference
Property (relative standard deviation is less than 4.26 %, N=5), be stored in 4 DEG C of environment, there is preferable stability (after 31 days still
There is the response of 93.9% initial current).
(2) glucose solution of various concentration is added dropwise, according to linear relationship curve, makes detection glucose accuracy
Clarke error analysis figure, as shown in Figure 4.In Fig. 4, the sample point of 66 detection glucose is measured, all sample points are complete
Portion has all been fallen in clinical accurate area (area A), and the coefficient of variation illustrates that the paper base electrode test piece prepared is used for less than 11.4%
Detection glucose is very accurately, ISO 15197-2013 standard to be complied fully with when detecting glucose.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (2)
1. a kind of preparation method of the paper base electrode detection platform for biochemical analysis, which is characterized in that described for biochemical point
The paper base electrode detection platform of analysis: using degradable paper wood as base material, it is printed on carbon electrode on base material, electron transmission is situated between
Matter and identification molecule, are fixed on the surface of paper base material carbon electrode by way of drop-coating, obtain the paper base electricity of Wholly-degradable
Pole detection platform；
Carbon electrode is prepared by screen printing technique, and moves into baking oven and is solidified, obtains paper base material carbon electrode；
The potassium ferricyanide solution that compound concentration is 50 mMs every liter is added dropwise the solution of 20~50 μ L in carbon electrodes, and puts
In 25 DEG C~30 DEG C of baking oven, save 10~30 minutes；
It is the glucose oxidase solution of 10K international unit, supplying to the carboxymethyl cellulose containing 0.05 gram per liter by activity
In 50 mMs every liter of phosphate buffer of sodium, the solution of 20~50 μ L is added dropwise in carbon electrodes, and be placed on 25 DEG C~30
DEG C baking oven in, save 10~30 minutes, prepare paper base electrode test piece；
The paper base electrode detection platform for biochemical analysis preparation method preparation paper base electrode detection platform by: identification
Molecular layer, electron transfer mediator layer, carbon electrode, paper base material composition；
Carbon electrode is placed on the paper base material, one end of carbon electrode is placed with electron transfer mediator layer, electron transfer mediator layer
On be placed with identification molecular layer；
The material of the paper base material is cellulose paper；
The paper base electrode detection platform is that the pectination finger-fork type carbon electrode on the base material is constituted.
2. a kind of biochemical method for detecting glucose, which is characterized in that the paper base comprising being used for biochemical analysis described in claim 1
The preparation method of electrode detection platform.
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| CN114557694B (en) * | 2022-04-28 | 2022-09-23 | 中国科学院大学 | Noninvasive subcutaneous tissue fluid extraction-detection device and extraction-detection method |
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| CN102565153A (en) * | 2011-12-27 | 2012-07-11 | 桂林乐尔医疗器械有限公司 | Electrode type urine glucose testing strip with function of pretreating detected sample |
| CN103175872A (en) * | 2011-12-23 | 2013-06-26 | 长沙中生众捷生物技术有限公司 | Portable electrochemical detection test strip and preparation method thereof |
| CN104535626A (en) * | 2014-12-01 | 2015-04-22 | 济南大学 | Preparation method and application of paper-based self-power supply biosensor |
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| US9702839B2 (en) * | 2011-03-11 | 2017-07-11 | Mc10, Inc. | Integrated devices to facilitate quantitative assays and diagnostics |
| WO2013036617A1 (en) * | 2011-09-06 | 2013-03-14 | President And Fellows Of Harvard College | Microfluidic devices for multiplexed electrochemical detection |
| US9714912B2 (en) * | 2012-08-13 | 2017-07-25 | Achira Labs Pvt. Ltd. | Compositions for fabric based lateral flow assay device using electrochemical detection means, and devices therefrom |
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| CN103175872A (en) * | 2011-12-23 | 2013-06-26 | 长沙中生众捷生物技术有限公司 | Portable electrochemical detection test strip and preparation method thereof |
| CN102565153A (en) * | 2011-12-27 | 2012-07-11 | 桂林乐尔医疗器械有限公司 | Electrode type urine glucose testing strip with function of pretreating detected sample |
| CN104535626A (en) * | 2014-12-01 | 2015-04-22 | 济南大学 | Preparation method and application of paper-based self-power supply biosensor |
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