CN105944154A - Composite structure small-caliber tubular porous scaffold and manufacturing method thereof - Google Patents
Composite structure small-caliber tubular porous scaffold and manufacturing method thereof Download PDFInfo
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- CN105944154A CN105944154A CN201610396911.XA CN201610396911A CN105944154A CN 105944154 A CN105944154 A CN 105944154A CN 201610396911 A CN201610396911 A CN 201610396911A CN 105944154 A CN105944154 A CN 105944154A
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- small
- caliber tubular
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/146—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/048—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/08—Materials for coatings
- A61L31/10—Macromolecular materials
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
- D01D5/0084—Coating by electro-spinning, i.e. the electro-spun fibres are not removed from the collecting device but remain integral with it, e.g. coating of prostheses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/18—Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2420/00—Materials or methods for coatings medical devices
- A61L2420/02—Methods for coating medical devices
Abstract
The invention relates to a composite structure small-caliber tubular porous scaffold and a manufacturing method thereof. An inner layer of the small-caliber tubular scaffold is of a porous structure, and an outer layer is an electrostatic spinning nanometer fiber layer. The preparation method comprises the steps that a mold tube material is filled with a pore-forming agent, and a pore-forming agent layer is attached to the inner wall of the tube material; a thermosetting polyester material solution is injected into the pore-forming agent layer, a solvent is volatilized, and crosslinking is performed under the temperature of 120-150 DEG C, the temperature is decreased to the room temperature, then, a supporting bar is moved in, the outer layer mold tube material is removed, and the inner layer structure of the small-caliber tubular scaffold is obtained; electrostatic spinning cladding is performed on the small-caliber tubular scaffold, the pore-forming agent is removed, freezing and drying are performed, the supporting bar is removed, and the small-caliber tubular scaffold on the basis of the composite material and the composite structure is obtained. The preparation method is easy, convenient and efficient to implement; a layer of electrostatic spinning is attached to the outer side of the obtained small-caliber tubular scaffold, errhysis of the inner layer porous structure is avoided, and the mechanical characteristic of the tubular scaffold is enhanced.
Description
Technical field
The invention belongs to tubular bracket and preparation field thereof, particularly to a kind of composite construction small-caliber tubular porous support and system thereof
Preparation Method.
Background technology
At present, clinical medicine needs substantial amounts of blood vessel graft or sub to carry out artificial blood vessel in the operations such as coronary bypass
The transplanting of support；Therefore, small-bore artificial blood vessel is more and more paid attention to.And for small-caliber vascular stent, biological
Bad being easier to of the compatibility causes thrombosis and restenosis, so the restenosis caused by thrombosis is small-caliber vascular group
One significant challenge of weaver's journey.Therefore, in order to meet Treatment need, researchers are devoted to be prepared the side of support by improvement
Method.There is loose structure preparation complexity, the problems such as row yielding is low in the research currently for small-caliber tubular support.
Electrostatic spinning technique is the common method preparing support in organizational project, and support specific surface area prepared by this kind of method is big, hole
Gap rate is high, and nanofiber diameter is suitable with internal many cell sizes, it is possible to growth factor-loaded inducing cell sticks, breed and
Differentiation, has peculiar advantage for Cell culture invitro, analog cell epimatrix structure.Therefore, electrostatic spinning technique gradually becomes
The common method of support is prepared for organizational project in applying.
Summary of the invention
The technical problem to be solved is to provide a kind of composite construction small-caliber tubular porous support and preparation method thereof, should
Method is easy, efficient, simplifies the preparation method of composite construction internal layer loose structure, improves the preparation of small-caliber tubular support
Success rate.
The invention provides a kind of simplicity, the method efficiently preparing the small-caliber tubular support of Material cladding, structure composite.In it
Layer loose structure uses salting out method to be processed molding, and material therefor is thermosetting polyester material.Outer layer Static Spinning material therefor is
The material of electrostatic spinning can be carried out.Two kinds of different methods, process the small-caliber tubular support of material and structure composite.
A kind of composite construction small-caliber tubular porous support of the present invention, the internal layer of described small-caliber tubular support is loose structure,
Outer layer is electrostatic spinning nano fiber layer.
A kind of preparation method of the composite construction small-caliber tubular porous support of the present invention, including:
(1) porogen is filled in the mould tubing of .5mm-10mm, with metal bar, unnecessary porogen is removed outside pipe,
Adhere to one layer of closely knit pore oxidant layer at pipe material inner wall, remove metal bar；
(2) thermosetting polyester material solution syringe is injected into the pore oxidant layer in step (1), fully infiltrates, volatilization
Solvent, 120-150 DEG C cross-links, and is down to room temperature, then moves into support bar, and removing outer layer mould tubing (will outward with solvent
Layer mould tubing dissolves), obtain small-caliber tubular support endothecium structure；
(3) small-caliber tubular support in step (2) is carried out Static Spinning cladding, remove porogen and (use solubilized porogen
Solvent soaking support dissolve porogen), lyophilization, remove support bar, obtain based on composite and composite construction little
Bore tubular bracket；Wherein, the internal layer of small-caliber tubular support is loose structure, and outer layer is electrostatic spinning nano fiber structure.
In described step (1) porogen be NaCl or other can water-soluble, nontoxic, there is the granule of certain form.
In described step (1), porogen carries out pre-treatment before using, and obtains the porogen having partial miscibility and being bonded together；
Described pre-treatment is: by perforating agent through grinding or high-speed stirred, and is filtered out by the screen cloth of certain mesh number and has specific grain
The perforating agent of footpath size.Required aperture perforating agent is processed 0.5-5 hour under certain humidity (50-95%), it is ensured that porogen
Between have certain bonding force.
Described humidity is 85%.
The pretreatment mode of described NaCl is: NaCl blender is smashed, and sifts out the salt particle of diameter 20～300 μm with screen cloth,
It is placed on climatic chamber 0.5-5h, design temperature 25-40 DEG C, humidity 50 95%.
In described step (1), mould tubing is: PMMA pipe (internal diameter 2.0mm) of long 0.5-104cm, outer layer PMMA
Effective chloroform dissolves；Described metal bar is: the metal bar (external diameter 1.6～1.7mm) of long 6cm.
In described step (2) thermosetting polyester material be sebacic acid and propyl tri-alcohol ester PGS, other polyprotic acid and polyhydric alcohol thing,
Bakelite, epoxy resin, aminoplast, unsaturated polyester (UP) or alkyd plastics etc.；The solvent of thermosetting polyester material solution is
THF, acetone, ethyl acetate, methanol, trifluoroethanol or hexafluoroisopropanol etc..
In described step (2), the time of crosslinking is 24-72h.
Supporting bar in described step (2) and step (3) is metal bar.
In described step (3) spinning liquid of Static Spinning be Poly(D,L-lactide-co-glycolide PLGA, polycaprolactone (PCL), poly-
The electrostatic spinning solution of lactic acid PLA, gelatin, collagen protein etc..
In described step (3), the condition of Static Spinning is: voltage 5-15KV, feeding speed 0.5-2ml/h, receiving range 5-15cm,
The spinning time is 0.5-2h, rotating speed 100-500r/min.
In described step (3), internal diameter based on composite and the small-caliber tubular support of composite construction is 1mm, and wall thickness is 0.5
mm。
The mould that porogen used in the present invention is commercially available, used includes tubing and bar, the Yi Jixiang of available solvent dissolving
Answer the metal bar of size.
Beneficial effect
(1) small-caliber tubular support prepared by the present invention simplifies the preparation method of composite construction internal layer loose structure, improves osculum
Footpath tubular bracket be prepared as power；
(2) the small-caliber tubular support that prepared by the present invention uses the outer layer tubular die that can be dissolved by the solvent, it is to avoid additive method takes off
Damage during mould, finished product caused；
(3) with one layer of electrostatic spinning outside small-caliber tubular support prepared by the present invention, it is to avoid the oozing of blood of internal layer loose structure,
Enhance the engineering properties of tubular bracket.
Accompanying drawing explanation
Fig. 1 is the sectional view of the tubular bracket observed under the microscope in embodiment 1；
Fig. 2 is the SEM figure of composite construction small-caliber tubular porous support in embodiment 1；The corresponding diverse location of 2A with 2B.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further.Should be understood that these embodiments be merely to illustrate the present invention and not
For limiting the scope of the present invention.In addition, it is to be understood that after having read the content that the present invention lectures, those skilled in the art can
To make various changes or modifications the present invention, these equivalent form of values fall within the application appended claims limited range equally.
Embodiment 1
NaCl blender is smashed, sifts out the salt particle of diameter 20～300 μ with screen cloth, be placed on climatic chamber 1h,
Design temperature 37 DEG C, humidity 85%.
Intercepting PMMA pipe (internal diameter 2.0mm) of long 6cm, the metal bar (external diameter 1.6～1.7mm) of long 6cm, by salt
Granule inserts PMMA pipe under said temperature humidity, removes outside pipe with supporting bar by unnecessary salt particle, by true for PMMA pipe
Empty drying.
To the THF solution (100mg/ml, 1ml syringe) of salt mold-in injection PGS (prepolymer, self-control), by PMMA
Pipe standing is volatilized completely to solvent.Enter vacuum drying oven crosslinking 24h, design temperature 120 DEG C, obtain PGS-salt mold layer.
Effective for outer layer PMMA chloroform is dissolved, the PGS-salt mold layer obtained is stood and volatilizees completely to chloroform, enter oven for drying.
PGS-salt mold layer is carried out Static Spinning cladding, and spinning liquid is PLGA (Mn=60000) hexafluoroisopropanol solution (concentration
150mg/ml, 5ml syringe) Electrospinning parameters is: voltage 10KV, feeding speed 1ml/h, receiving range 15cm,
The spinning time is 0.5h, rotating speed 300r/min.
After Static Spinning terminates, salt mould need to be dissolved lyophilization with distilled water immersion 48h by support, removes support bar, must answer
Close structure small-caliber tubular porous support.
Claims (10)
1. a composite construction small-caliber tubular porous support, it is characterised in that the internal layer of described small-caliber tubular support is porous knot
Structure, outer layer is electrostatic spinning nano fiber layer.
2. a preparation method for composite construction small-caliber tubular porous support, including:
(1) porogen is filled in mould tubing, adheres to one layer of pore oxidant layer at pipe material inner wall；
(2) thermosetting polyester material solution is injected into the pore oxidant layer in step (1), solvent flashing, hands over for 120-150 DEG C
Connection, is down to room temperature, then moves into support bar, removes outer layer mould tubing, obtains small-caliber tubular support endothecium structure；
(3) small-caliber tubular support in step (2) is carried out Static Spinning cladding, remove porogen, lyophilization, remove and prop up
Support bar, obtains the small-caliber tubular support based on composite and composite construction；Wherein, the internal layer of small-caliber tubular support is
Loose structure, outer layer is electrostatic spinning nano fiber structure.
The preparation method of a kind of composite construction small-caliber tubular porous support the most according to claim 2, it is characterised in that institute
Stating porogen in step (1) is NaCl.
The preparation method of a kind of composite construction small-caliber tubular porous support the most according to claim 2, it is characterised in that institute
State porogen in step (1) and before using, carry out pre-treatment: by porogen through grinding or stirring, and screened by screen cloth,
Process 0.5-5 hour under humidity 50-95%.
The preparation method of a kind of composite construction small-caliber tubular porous support the most according to claim 2, it is characterised in that institute
State thermosetting polyester material in step (2) be sebacic acid and propyl tri-alcohol ester PGS, bakelite, epoxy plastics, aminoplast,
Unsaturated polyester (UP) or alkyd plastics；The solvent of thermosetting polyester material solution is THF, acetone, ethyl acetate, methanol, trifluoro
Ethanol or hexafluoroisopropanol.
The preparation method of a kind of composite construction small-caliber tubular porous support the most according to claim 2, it is characterised in that institute
The time of crosslinking in step (2) of stating is 24-72h.
The preparation method of a kind of composite construction small-caliber tubular porous support the most according to claim 2, it is characterised in that institute
Stating and supporting bar in step (2) and step (3) is metal bar.
The preparation method of a kind of composite construction small-caliber tubular porous support the most according to claim 2, it is characterised in that institute
State the composition of the spinning liquid of Static Spinning in step (3) be Poly(D,L-lactide-co-glycolide PLGA, polycaprolactone (PCL), poly-
Lactic acid PLA, gelatin or collagen protein.
The preparation method of a kind of composite construction small-caliber tubular porous support the most according to claim 2, it is characterised in that institute
The condition of Static Spinning in step (3) of stating is: voltage is 5-15KV, and feeding speed is 0.5-2ml/h, and receiving range is 5-15cm,
The spinning time is 0.5-2h, and rotating speed is 100-500r/min.
The preparation method of a kind of composite construction small-caliber tubular porous support the most according to claim 2, it is characterised in that institute
Stating internal diameter based on composite and the small-caliber tubular support of composite construction in step (3) is 1mm, and wall thickness is 0.5mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610396911.XA CN105944154A (en) | 2016-06-06 | 2016-06-06 | Composite structure small-caliber tubular porous scaffold and manufacturing method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610396911.XA CN105944154A (en) | 2016-06-06 | 2016-06-06 | Composite structure small-caliber tubular porous scaffold and manufacturing method thereof |
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| CN105944154A true CN105944154A (en) | 2016-09-21 |
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| CN201610396911.XA Pending CN105944154A (en) | 2016-06-06 | 2016-06-06 | Composite structure small-caliber tubular porous scaffold and manufacturing method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112043869A (en) * | 2020-08-19 | 2020-12-08 | 南方医科大学 | Preparation method of elastic and plastic polyester material and elastic and plastic polyester material |
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| CN103394125A (en) * | 2013-07-11 | 2013-11-20 | 东华大学 | Tissue engineering double-layered tubular support and preparation method thereof |
| CN105363076A (en) * | 2015-09-30 | 2016-03-02 | 东华大学 | Method for preparing polylactic acid caprolactone-collagen two-layer bionic vascular scaffold |
| CN105457101A (en) * | 2015-12-17 | 2016-04-06 | 华南理工大学 | Preparation method of small-caliber intravascular stent of three-layer structure |
-
2016
- 2016-06-06 CN CN201610396911.XA patent/CN105944154A/en active Pending
Patent Citations (4)
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|---|---|---|---|---|
| US20090012607A1 (en) * | 2007-07-06 | 2009-01-08 | Korea Institute Of Science And Technology | Method for the preparation of tube-type porous biodegradable scaffold having double-layered structure for vascular graft |
| CN103394125A (en) * | 2013-07-11 | 2013-11-20 | 东华大学 | Tissue engineering double-layered tubular support and preparation method thereof |
| CN105363076A (en) * | 2015-09-30 | 2016-03-02 | 东华大学 | Method for preparing polylactic acid caprolactone-collagen two-layer bionic vascular scaffold |
| CN105457101A (en) * | 2015-12-17 | 2016-04-06 | 华南理工大学 | Preparation method of small-caliber intravascular stent of three-layer structure |
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|---|
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112043869A (en) * | 2020-08-19 | 2020-12-08 | 南方医科大学 | Preparation method of elastic and plastic polyester material and elastic and plastic polyester material |
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