CN105943037A - Myoelectricity detection device combined with laparoscope and operating method thereof - Google Patents

Myoelectricity detection device combined with laparoscope and operating method thereof Download PDF

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Publication number
CN105943037A
CN105943037A CN201610342553.4A CN201610342553A CN105943037A CN 105943037 A CN105943037 A CN 105943037A CN 201610342553 A CN201610342553 A CN 201610342553A CN 105943037 A CN105943037 A CN 105943037A
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electrode
checking
emg method
epoxy resin
laparoscopically
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CN105943037B (en
Inventor
杜爱林
成竹影
姜洪波
张玉姣
严静
路承彪
王昊阳
张越
李文杰
代玄
张璐
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Third Affiliated Hospital of Xinxiang Medical University
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Third Affiliated Hospital of Xinxiang Medical University
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Measuring for diagnostic purposes; Identification of persons
    • A61B5/24Detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof
    • A61B5/316Modalities, i.e. specific diagnostic methods
    • A61B5/389Electromyography [EMG]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/012Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor characterised by internal passages or accessories therefor
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/31Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor for the rectum, e.g. proctoscopes, sigmoidoscopes, colonoscopes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/313Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor for introducing through surgical openings, e.g. laparoscopes
    • A61B1/3132Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor for introducing through surgical openings, e.g. laparoscopes for laparoscopy
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Measuring for diagnostic purposes; Identification of persons
    • A61B5/24Detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof
    • A61B5/25Bioelectric electrodes therefor
    • A61B5/279Bioelectric electrodes therefor specially adapted for particular uses
    • A61B5/296Bioelectric electrodes therefor specially adapted for particular uses for electromyography [EMG]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Measuring for diagnostic purposes; Identification of persons
    • A61B5/42Detecting, measuring or recording for evaluating the gastrointestinal, the endocrine or the exocrine systems
    • A61B5/4222Evaluating particular parts, e.g. particular organs
    • A61B5/4255Intestines, colon or appendix
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Measuring for diagnostic purposes; Identification of persons
    • A61B5/68Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
    • A61B5/6846Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive
    • A61B5/6847Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive mounted on an invasive device
    • A61B5/6848Needles
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Measuring for diagnostic purposes; Identification of persons
    • A61B5/68Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
    • A61B5/6846Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive
    • A61B5/6847Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive mounted on an invasive device
    • A61B5/6852Catheters
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Measuring for diagnostic purposes; Identification of persons
    • A61B5/68Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
    • A61B5/6846Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive
    • A61B5/6867Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive specially adapted to be attached or implanted in a specific body part
    • A61B5/6873Intestine
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B2562/00Details of sensors; Constructional details of sensor housings or probes; Accessories for sensors
    • A61B2562/02Details of sensors specially adapted for in-vivo measurements
    • A61B2562/0209Special features of electrodes classified in A61B5/24, A61B5/25, A61B5/283, A61B5/291, A61B5/296, A61B5/053
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B2562/00Details of sensors; Constructional details of sensor housings or probes; Accessories for sensors
    • A61B2562/12Manufacturing methods specially adapted for producing sensors for in-vivo measurements
    • A61B2562/125Manufacturing methods specially adapted for producing sensors for in-vivo measurements characterised by the manufacture of electrodes

Abstract

The invention discloses a myoelectricity detection device combined with a laparoscope and an operating method thereof. The myoelectricity detection device comprises a working mirror tube, the laparoscope and a myoelectricity detection electrode. The interior of the working mirror tube is provided with an imaging channel and an electrode channel, both of which are arranged in parallel. The laparoscope is arranged in the imaging channel. The myoelectricity detection electrode is arranged in the electrode channel and comprises a medical cannula needle head, an electrode and an inner cannula and an outer cannula. The inner cannula and the outer cannula differ in internal diameters and are sleeved with each other. The medical cannula needle head is fixed at one end of the outer cannula. The electrode is fixed in the inner cannula. An electrode on one side of the inner cannula is guided into the medical cannula needle head and the front end of the electrode is aligned with the needle point end of the medical cannula needle head. The tail end of an electrode of the inner cannula is connected with a biology signal acquisition system through a wire. The myoelectricity detection device combined with the laparoscope and the operating method thereof have following beneficial effects: an operation wound is small; patient's pain can be effectively mitigated; postoperative recovery is quick; and interference factors caused by an operation are reduced such that an experiment result is more convincing.

Description

One combines laparoscopically checking with EMG method device and operational approach thereof
Technical field
The invention belongs to digestive tract detection medical applications technical field, be specifically related to one and combine laparoscopically checking with EMG method device and operational approach thereof.
Background technology
The myoelectricity action potential of colon results from slow wave, consistent with smooth muscle contraction, is the major impetus of pushability motion, thus colonic muscle electrical activity is as an objective electrophysiological index, can indirectly reflect colonic activity situation.Slow wave is a kind of periodically electrical activity of relative rule, controls the contraction rhythm and pace of moving things of intestinal, no matter whether shrinks and exist all the time.At present, the record of myoelectrical activity is one of important method of colonic activity research.In some is tested, because normal bowel mucosa is contained within a lot of blood vessel, mirror taking on a red color, it is difficult to when trace is hemorrhage differentiate, and the generation of some digestive tract disease therefore by Electromyographic detection, can help the activity making a definite diagnosis and identifying intestinal tube of disease with the change of Intestinal myoelectric activity.The common Melanosis coli of digestive system is a kind of optimum large intestine disease of non-inflammation being characterized with colorectal mucosa pigmentation, can be found the pathological changes outward appearance of its color, morphological variation by laparoscopically inspection.Finding through correlational study, Melanosis coli also can cause the appearance of constipation symptom, and the generation of constipation can cause the change of Intestinal myoelectric activity, peritoneoscope and checking with EMG method to have certain meaning in Differential Diagnosis.Additionally, checking with EMG method also can help to differentiate the activity of intestinal tube, assisted laparoscope inspection.The visual inspections using peritoneoscope to carry out digestive system more, the precedent the most peritoneoscope not combined with myenteron electro-detection.
Common gastrointestinal myoelectricity electrode many employings tin electrode, tin electrode material is softer, the hardest, and fusing point is relatively low, is difficult to welding after broken string.In addition, on electrode, many aids such as the insulation tubes such as polrvinyl chloride or connection medical suture needle that can overlap are guided, insulation tube and medical suture needle can produce destructive bigger irreversibility impact to intestinal tube wall, cause that bigger interference ripple occurs on electromyogram, therefore can not obtain ideal experimental result.
Summary of the invention
Present invention solves the technical problem that there is provided a kind of simple in construction, reasonable in design and detection combines laparoscopically checking with EMG method device accurately.
nullThe present invention solves that above-mentioned technical problem adopts the following technical scheme that,One combines laparoscopically checking with EMG method device,It is characterized in that including work telescope、Peritoneoscope and checking with EMG method electrode,The inside of the telescope that wherein works is provided with the imaging band and electrode channel being parallel to each other,Peritoneoscope is arranged in imaging band,Checking with EMG method electrode is arranged in electrode channel,This checking with EMG method electrode includes medical trocar head、Electrode and different and mutually intussusceptions the inner sleeves of two internal diameters and trocar sheath,The material of this inner sleeve and trocar sheath is epoxy resin,Medical trocar head is fixed on one end of trocar sheath by medical adhesive,The pintle material of this medical trocar head is ambroin,The external electrical of its forward metal needle tubing is coated with insulating barrier,Electrode is fixed on inner sleeve,In the electrode importing medical trocar head of inner sleeve side and the front end of this electrode and the needle tip flush of medical trocar head,The electrode tail end of inner sleeve opposite side is connected with Biological Signal Collecting System by wire.
Further preferably, a diameter of 5-11mm of described work telescope, a length of 20-50cm, a diameter of 3.5-7.7mm of imaging band, a diameter of 1.5-3.3mm of electrode channel.
Further preferably, described electrode is epoxy resin/graphite alkene/Nanometer Copper composite electrode, silver electrode, silver-silver chloride electrode, silver-nickel electrode or copper alloy silver-coated electrode, electrode two ends for processing through insulating need to make its two ends be respectively provided with electric conductivity through exposed process, and the exposed length at electrode two ends is respectively 0.5-5cm.
Further preferably, the concrete preparation process of described epoxy resin/graphite alkene/Nanometer Copper composite electrode is: ultrasonic after being mixed with distilled water by graphene oxide obtain graphene oxide colloidal sol;Graphene oxide colloidal sol and copper sulfate solution are placed in reaction vessel in room temperature sonic oscillation 1h, it is subsequently placed in the water-bath of 70 DEG C and is sequentially added into hydrazine hydrate aqueous solution and sodium hydrate aqueous solution under conditions of stirring, be naturally down to room temperature after continuously stirred 45min and obtain loading the Graphene of copper nanoparticle;The graphene dispersion of load copper nanoparticle is obtained epoxy resin/graphite alkene/Nanometer Copper mixture in epoxy resin E-51;Utilize firming agent polyamide 6 51 that epoxy resin/graphite alkene/Nanometer Copper mixture is carried out solidification and obtain epoxy resin/graphite alkene/Nanometer Copper composite, this epoxy resin/graphite alkene/Nanometer Copper composite processes through insulating and obtains electrode, and the electrode two ends that insulating processes need to make its two ends be respectively provided with electric conductivity through exposed process.
Further preferably, described electrode diameter is 0.5mm, a diameter of 1mm of inner sleeve, a diameter of 3mm of trocar sheath, and the length of this inner sleeve and trocar sheath is all higher than the length of work telescope.
Further preferably, described Biological Signal Collecting System is PowerLab data acquisition and analysis system.
The operational approach of combination of the present invention laparoscopically checking with EMG method device, it is characterised in that concretely comprise the following steps: inserted by peritoneoscope in the imaging band of work telescope, inserts checking with EMG method electrode in the electrode channel of work telescope;Manufacture of intraocular pneumoperitoneum, sends in digestive tract to be checked by work telescope according to conventional methods, when arriving look-out station, chooses target area by laparoscopically guiding;Touch the end being trapped in external checking with EMG method electrode jacket pipe, make medical trocar head penetrate intestinal wall until sarcoplasm layer, under laparoscopically auxiliary, thrust the degree of depth according to what medical trocar head end scale adjusted medical trocar head;Fixing it be trapped in external checking with EMG method electrode inner sleeve end and do not move, outwards gently draw the end of checking with EMG method electrode jacket pipe, the electrode front end making medical trocar head withdraw from an organization and to be fixed on checking with EMG method electrode inner sleeve is detained in the tissue, records electromyogram by bio signal sampling system.
The present invention compared with prior art has the advantages that
The present invention uses epoxy resin/graphite alkene/Nanometer Copper composite to make electrode, its epoxy resin has excellent electrical insulating property, resistant to chemical media and cementability, Graphene has electric conductivity and the biocompatibility of excellence, it is the material that intensity known to the mankind is the highest, it is evenly dispersed in epoxy resin the toughness that can increase epoxy resin, Nanometer Copper has good electric conductivity and relatively low electron mobility, and it is evenly dispersed in epoxy resin the electric conductivity that can improve epoxy resin well.Epoxy resin/graphite alkene/Nanometer Copper composite summary advantage, it is made into electrode and is provided simultaneously with excellent electric conductivity and excellent mechanical performance, use medical trocar head auxiliary to be placed in by electrode and surveyed in tissue, destructiveness and interference are less, and myenteron electricity is effectively combined with peritoneoscope, achieving effective combination of Microscopic observation and two indexs of checking with EMG method, this will be a strategic structural in checking with EMG method electrode investigative technique evolution.Can be used for diagnosing Animal diseases in animal experiment, as experiment proves that, can put into clinic also will produce good economic benefit and social benefit.
The operation wound of the present invention is little, it is possible to effectively alleviate patient's misery, and post-operative recovery is very fast, reducing because performing the operation the interference factor caused, making experimental result have more cogency.
Accompanying drawing explanation
Fig. 1 is the horizontal schematic diagram of telescope of working in the present invention;
Fig. 2 is the longitudinal schematic diagram of telescope of working in the present invention;
Fig. 3 is the schematic diagram of checking with EMG method electrode in the present invention;
Fig. 4 is the structural representation of medical trocar head in the present invention.
In figure: 1, work telescope, 2, imaging band, 3, electrode channel, 4, medical trocar head, 5, electrode, 6, inner sleeve, 7, trocar sheath, 8, PowerLab data acquisition and analysis system, 9, metal needle tubing, 10, pintle.
Detailed description of the invention
In order to be more fully understood that the technique scheme of the present invention, with embodiment, the present invention is described in further detail below in conjunction with the accompanying drawings.
Embodiment
The subjects of the present embodiment is rabbit.
The preparation of epoxy resin/graphite alkene/Nanometer Copper composite electrode
1, preparing graphene oxide colloidal sol: prepare graphene oxide by traditional Hummers method, the ultrasonic graphene oxide colloidal sol that obtains after being mixed with distilled water by graphene oxide, in graphene oxide colloidal sol, the concentration of graphene oxide is 0.01g/mL;
2, graphene oxide-loaded nano copper powder: by CuSO4·5H2nullO adds and ultrasonic in distilled water obtains copper sulfate solution to being completely dissolved,Graphene oxide colloidal sol step 1 prepared and copper sulfate solution are poured in the there-necked flask of 250mL,Room temperature sonic oscillation 1h,It is then placed within low whipping speed in the water-bath of 70 DEG C and under conditions of being 300r/min, is sequentially added into hydrazine hydrate aqueous solution and sodium hydrate aqueous solution,Naturally it is down to room temperature after continuing to stir 45min under conditions of low whipping speed is 300r/min in the water-bath of 70 DEG C and obtains loading the Graphene of copper nanoparticle,The concentration of described copper sulfate solution is 0.0267g/mL,The concentration of sodium hydrate aqueous solution is 0.05g/mL,The volumetric concentration of hydrazine hydrate aqueous solution is 61.54%,The volume ratio of graphene oxide colloidal sol and copper sulfate solution is 1:2,Hydrazine hydrate aqueous solution is 1:2.3 with the volume ratio of copper sulfate solution,Sodium hydrate aqueous solution is 1:3 with the volume ratio of copper sulfate solution;
3, the Graphene of load copper nanoparticle dispersion in the epoxy: the Graphene of load copper nanoparticle step 2 obtained joins in epoxy resin, 5-7min is disperseed to obtain epoxy resin/graphite alkene/Nanometer Copper mixture under conditions of rotating speed is 7000r/min with mulser, described epoxy resin is E-51, and the Graphene of load copper nanoparticle is 1:10 with the mass ratio of epoxy resin;
null4、Solidification: the inner surface at Teflon mould coats fine vacuum silicone grease,Then inner surface is scribbled the Teflon mould of fine vacuum silicone grease、Epoxy resin/graphite alkene/Nanometer Copper mixture and firming agent that step 3 obtains are respectively put in the baking oven of 60 DEG C insulation 2h,Then under conditions of rotating speed is 100r/min, stirs 4-5min after preheated epoxy resin/graphite alkene/Nanometer Copper mixture and preheated firming agent being mixed obtains epoxy resin/graphite alkene/Nanometer Copper prepolymer,Epoxy resin/graphite alkene/Nanometer Copper prepolymer is poured in the Teflon mould that preheated inner surface scribbles fine vacuum silicone grease,2h it is incubated under conditions of temperature is 70 DEG C,It is warming up to 125 DEG C again and is incubated 2.5h,It is continuously heating to 150 DEG C and is incubated 1h,The most naturally it is down to room temperature and obtains epoxy resin/graphite alkene/Nanometer Copper composite,This epoxy resin/graphite alkene/Nanometer Copper composite processes through insulating and obtains electrode,The electrode two ends that insulating processes need to make its two ends be respectively provided with electric conductivity through exposed process,Described firming agent is polyamide 6 51,The mass ratio of epoxy resin used is 1:2 to firming agent with step 3.
Seeing Fig. 1-2, the inside of described work telescope 1 is provided with the imaging band 2 and electrode channel 3 being parallel to each other, a diameter of 5-11mm, a length of 20-50cm, a diameter of 3.5-7.7mm of imaging band 2, a diameter of 1.5-3.3mm of electrode channel 3 of work telescope 1.
See Fig. 3, described checking with EMG method electrode includes medical trocar head 4, electrode 5 and different and mutually intussusceptions the inner sleeves 6 of two internal diameters and trocar sheath 7, the material of this inner sleeve 6 and trocar sheath 7 is epoxy resin, medical trocar head 4 is fixed on one end of trocar sheath 7 by biogum, pintle 10 material of this medical trocar head 4 is ambroin, the external electrical of its forward metal needle tubing 9 is coated with insulating barrier, electrode 5 is fixed on inner sleeve 6, the two ends of this electrode 5 make its two ends be respectively provided with electric conductivity through exposed process, the electrode 5 of inner sleeve 6 side imports in medical trocar head 4 and the needle tip flush of front end and medical trocar head 4 of this electrode 5, electrode 5 tail end of inner sleeve 6 opposite side is connected with PowerLab data acquisition and analysis system 8 by wire.
In use, peritoneoscope is inserted in the imaging band 2 of work telescope 1, checking with EMG method electrode is inserted in the electrode channel 3 of work telescope 1;Manufacture of intraocular pneumoperitoneum, sends in digestive tract to be checked by work telescope 1 according to conventional methods, when arriving look-out station, chooses target area by laparoscopically guiding;Touch the end being trapped in external checking with EMG method electrode jacket pipe 7, make medical trocar head 4 penetrate intestinal wall until sarcoplasm layer, under laparoscopically auxiliary, thrust the degree of depth according to what the scale on medical trocar head adjusted medical trocar head 4;Fixing it be trapped in external checking with EMG method electrode inner sleeve 6 end and do not move, outwards gently draw the end of checking with EMG method electrode jacket pipe 7, the electrode front end making medical trocar head 4 withdraw from an organization and to be fixed on checking with EMG method electrode inner sleeve 6 is detained in the tissue, records electromyogram by PowerLab data acquisition and analysis system 8.
The simply explanation principle of the present invention, principal character and advantage described in above-described embodiment and description, under the scope without departing from the principle of the invention, the present invention also has various changes and modifications, and these changes and improvements each fall within the scope of protection of the invention.

Claims (7)

  1. null1. one kind combines laparoscopically checking with EMG method device,It is characterized in that including work telescope、Peritoneoscope and checking with EMG method electrode,The inside of the telescope that wherein works is provided with the imaging band and electrode channel being parallel to each other,Peritoneoscope is arranged in imaging band,Checking with EMG method electrode is arranged in electrode channel,This checking with EMG method electrode includes medical trocar head、Electrode and different and mutually intussusceptions the inner sleeves of two internal diameters and trocar sheath,The material of this inner sleeve and trocar sheath is epoxy resin,Medical trocar head is fixed on one end of trocar sheath by biogum,The pintle material of this medical trocar head is ambroin,The external electrical of its forward metal needle tubing is coated with insulating barrier,Electrode is fixed on inner sleeve,In the electrode importing medical trocar head of inner sleeve side and the front end of this electrode and the needle tip flush of medical trocar head,The electrode tail end of inner sleeve opposite side is connected with Biological Signal Collecting System by wire.
  2. Combination the most according to claim 1 laparoscopically checking with EMG method device, it is characterised in that: a diameter of 5-11mm of described work telescope, a length of 20-50cm, a diameter of 3.5-7.7mm of imaging band, a diameter of 1.5-3.3mm of electrode channel.
  3. Combination the most according to claim 1 laparoscopically checking with EMG method device, it is characterized in that: described electrode is epoxy resin/graphite alkene/Nanometer Copper composite electrode, silver electrode, silver-silver chloride electrode, silver-nickel electrode or copper alloy silver-coated electrode, electrode two ends for processing through insulating need to make its two ends be respectively provided with electric conductivity through exposed process, and the exposed length at electrode two ends is respectively 0.5-5cm.
  4. Combination the most according to claim 3 laparoscopically checking with EMG method device, it is characterised in that the concrete preparation process of described epoxy resin/graphite alkene/Nanometer Copper composite electrode is: ultrasonic after being mixed with distilled water by graphene oxide obtain graphene oxide colloidal sol;Graphene oxide colloidal sol and copper sulfate solution are placed in reaction vessel in room temperature sonic oscillation 1h, it is subsequently placed in the water-bath of 70 DEG C and is sequentially added into hydrazine hydrate aqueous solution and sodium hydrate aqueous solution under conditions of stirring, be naturally down to room temperature after continuously stirred 45min and obtain loading the Graphene of copper nanoparticle;The graphene dispersion of load copper nanoparticle is obtained epoxy resin/graphite alkene/Nanometer Copper mixture in epoxy resin E-51;Utilize firming agent polyamide 6 51 that epoxy resin/graphite alkene/Nanometer Copper mixture is carried out solidification and obtain epoxy resin/graphite alkene/Nanometer Copper composite, this epoxy resin/graphite alkene/Nanometer Copper composite processes through insulating and obtains electrode, and the electrode two ends that insulating processes need to make its two ends be respectively provided with electric conductivity through exposed process.
  5. Combination the most according to claim 1 laparoscopically checking with EMG method device, it is characterised in that: described electrode diameter is 0.5mm, a diameter of 1mm of inner sleeve, a diameter of 3mm of trocar sheath, and the length of this inner sleeve and trocar sheath is all higher than the length of work telescope.
  6. Combination the most according to claim 1 laparoscopically checking with EMG method device, it is characterised in that: described Biological Signal Collecting System is PowerLab data acquisition and analysis system.
  7. 7. the operational approach of the combination laparoscopically checking with EMG method device described in a claim 1, it is characterised in that concretely comprise the following steps: peritoneoscope is inserted in the imaging band of work telescope, checking with EMG method electrode is inserted in the electrode channel of work telescope;Manufacture of intraocular pneumoperitoneum, sends in digestive tract to be checked by work telescope according to conventional methods, when arriving look-out station, chooses target area by laparoscopically guiding;Touch the end being trapped in external checking with EMG method electrode jacket pipe, make medical trocar head penetrate intestinal wall until sarcoplasm layer, under laparoscopically auxiliary, thrust the degree of depth according to what medical trocar head end scale adjusted medical trocar head;Fixing it be trapped in external checking with EMG method electrode inner sleeve end and do not move, outwards gently draw the end of checking with EMG method electrode jacket pipe, the electrode front end making medical trocar head withdraw from an organization and to be fixed on checking with EMG method electrode inner sleeve is detained in the tissue, records electromyogram by bio signal sampling system.
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