CN105929149A - Optical detector based on magnetic enrichment and total internal reflection - Google Patents
Optical detector based on magnetic enrichment and total internal reflection Download PDFInfo
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- CN105929149A CN105929149A CN201610266441.5A CN201610266441A CN105929149A CN 105929149 A CN105929149 A CN 105929149A CN 201610266441 A CN201610266441 A CN 201610266441A CN 105929149 A CN105929149 A CN 105929149A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N2021/557—Detecting specular reflective parts on sample
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Abstract
The invention discloses an optical detector based on magnetic enrichment and total internal reflection. The optical detector is characterized in that the optical detector comprises an electric control two-magnetic field enrichment and separation system, an optical detection system and a constant temperature heating system, and the electric control two-magnetic field enrichment and separation system is used for generating alternative magnetic fields to realize bidirectional driving and enrichment of magnetic particles on a chip; the optical detection system adopts a total internal reflection detection technology to realizes detection and analysis of an optical image; and the constant temperature heating system comprises a temperature sensor and a heating plate, and is used for controlling the temperature of detection environment. The detector adopting an electric control two-magnetic field technology, a total internal reflection optical detection technology and an image analysis software technology realizes automatic and rapid detection of low-concentration substances, portability, one-step operation, and high simplicity and convenience.
Description
Technical field
The invention belongs to technical field of biomedical detection, be specifically related to a kind of interior with complete based on magnetic enrichment
The optical detector of reflection.
Background technology
Low concentration material detection (especially in blood sample) in sample, generally uses immune detection skill
Art.In order to reduce interference, it is necessary first to before blood sample is centrifuged separation, removal hemocyte etc.
Process step, which adds detection time and detecting step.In order to increase detection accuracy and
Susceptiveness, a lot of immunoreation use magnetic separate particles technology by the thing to be detected of low concentration from sample
Separate, it is also desirable to magnetic particle composite out and is washed by centrifugation, detection spirit
Sensitivity is high, but complex steps.And, the instrument of use is huge and expensive, operation complexity, cost
Height, and need the reviewer of specialty to operate, limit detection time and detection place, it is impossible to
For basic unit and Site Detection.
Increasing of microdose urine protein (urinary microalbumin, english abbreviation: mAlb) is common
In diabetic nephropathy, hypertension, pre-eclampsia, it it is the early stage sensitive indicator of injury of kidney.If
After health check-up, find that the microalbumin in urine, in the range of 20mg/L-200mg/L, just belongs to trace
Albuminuria, if patient can be through the reparation nephron of specification, reverse fibrosis is treated, Shang Ke
Thoroughly repair glomerule, eliminate albuminuria.And when in urine, microalbumin is more than 200mg/L, this
Time prove that nephrotic's a large amount of albumin spill, development of renal disease only has one step away from the irreversible phase,
If cured not in time, Uremic will be entered.
Summary of the invention
In view of this, it is an object of the invention to prepare a kind of optics inspection based on magnetic enrichment and total internal reflection
Survey instrument.The detector of the present invention uses automatically controlled pair of magnetic field technique, total internal reflection optical detection technique and figure
As analyzing software engineering, it is achieved the automatically quickly detection of low concentration material in sample, instrument is portable, one
Step operation, simple and fast.
For achieving the above object, the present invention provides a kind of based on magnetic enrichment and the optical detection of total internal reflection
Instrument, comprising: the automatically controlled pair of magnetic field concentration and separation system, Systems for optical inspection and constant-temperature heating system,
Described automatically controlled pair of magnetic field concentration and separation system is for producing magnetic field alternately, to realize magnetic grain on chip
The bi-directional drive of son and enrichment；Described Systems for optical inspection uses total internal reflection detection technique, to realize
The detection of optical imagery and analyzing and processing；Described constant-temperature heating system includes temperature sensor and heating plate,
For controlling to detect the temperature of environment.
Described automatically controlled pair of magnetic field concentration and separation system is (little by being placed on the upper and lower both sides of detection chip
Type is strengthened) magnetic post or magnetic sheet, alternately power up/down produces magnetic field alternately, to realize on chip
The bi-directional drive of magnetic particle and enrichment.Preferably, described automatically controlled pair of magnetic field concentration and separation system includes:
(small-sized) magnetic post that power supply, electromagnetic control circuit program and two are identical.
Wherein, described electromagnetic control circuit program can realize according to the frequency set respectively to two magnetic
Post powers up generation magnetic and de-electrifying elimination magnetic, thus realizes separation and the enrichment of magnetic particle.
Preferably, the time range powered up described in is 0.1～30 second；Cycle period is 0～100 cycle；
It is 0.5～100s for last the time of staying of magnetic separate particles；The intensity powered up is 0.1～50V.
The time range powered up described in it is highly preferred that is 2～10 seconds；Cycle period is 5～20 cycles；With
It is 5～20s in last the time of staying of magnetic separate particles.
In one preferably example, two of which small size magnetic post is positioned at the both sides of detection chip, needs
Join the suitable little coil of optical detection tank, produce maximum magnetic field to realize the magnet of minimum, small-sized
Magnetic post is to be powered generation magnetic field and the coil in de-electrifying elimination magnetic field, and has and add ferromagnetic iron core
Or cobalt ferronickel core.
Described Systems for optical inspection uses total internal reflection detection technique, and it includes launching light path and detection light
Road two parts.
Wherein, described transmitting light path is mainly light source emitting module, its comprise control circuit for light source plate and
Program, LED or parallel laser light source.
Wherein the intensity of LED or laser can be set by control circuit for light source plate and program,
Wherein electric current adjusting range preferably 0～1mA；Connect by RS485 by key-press input or host computer
Mouth amendment LED current numerical value, and then change the brightness of light source.
Described LED or parallel laser light source include the parts such as beam expanding lens and diaphragm, dissipating of light source
The parameter such as angle, hot spot can be regulated by modes such as collimation, diaphragms, forms a branch of uniform directional light.
Wherein, described parallel laser light source uses red collimated light beam, wavelength preferably 600～650nm.
Wherein, described detection light path include optical detecting module, image recognition software, microlens or
Telecentric lens.
Wherein, described optical detecting module can use CCD, CMOS, light emitting diode or photoelectricity
Multiplier tubes etc. carry out optical imagery detection, wherein preferably CCD camera, more preferably CCD camera
Pixel is more than 1,000,000 pixels；Simultaneously need to coordinate microlens or telecentric lens to carry out image
Shooting.The present invention preferred optically detecting speed and resolution reach to detect the image of micron-scale.
In one preferably example, the core collecting part of described transmitting light path and detection light path uses dark
Box is closed, and magazine carries out inwall blackening process, eliminates interference of stray light, incident angle and acceptance
The angle of light path is different with chip material change, and material determines, angle is fixed.
Wherein it is preferred to, it is right that described image recognition software uses the softwares such as MATLAB or C to realize
The display of image, storage and the contrast of gray scale discriminatory analysis and dimensional analysis.
Described constant-temperature heating system includes temperature sensor and heating plate.Constant-temperature heating system passes through temperature
Sensor and heating plate control the temperature of detection environment, are preferably controlled in 37 DEG C of temperature constant states, so that exempting from
Epidemic disease reaction is carried out under specified temp environment, eliminates the response difference error that temperature causes.
Temperature sensor obtains conversion zone temperature data in real time, then uses pid algorithm to calculate and adds
Backing voltage exporting change amount, and by the heating voltage after the output adjustment of heating plate drive circuit, it is achieved
Accurate control to regional temperature.
The present invention is when detection, and concrete operations are preferably: add chip, preheater apparatus, arrange magnetic field
With the appropriate value of light source, adding sample, instrument automatically begins to enter detection pattern, detects complete,
Image taking, software identification image, and according to pre-calibration value, calculate the testing concentration in sample,
Display numerical value.
In the present invention, detection sample can be whole blood, blood plasma, serum, urine, saliva, body fluid
Deng.The present invention uses immune detection principle, it is adaptable to the thing to be checked that in sample, content is low.On chip
Fixing Multiple Antibodies, detector can realize the multiple indexs of chip last time detection.
The chip being applicable to detector of the present invention can be the plastic material of high transparency, glass or crystal material
Material etc..
The present invention has a following beneficial effect:
(1) present invention uses automatically controlled pair of magnetic field concentration and separation pattern, is effectively increased response speed,
Simplify experimental procedure, it is achieved that single step detects.
(2) the total internal reflection optical detection technique that the present invention uses combines magnetic particle technique, it is achieved that
The portable design of instrument, instrument is simple and effective, beneficially Site Detection, therefore has extraordinary answering
Use prospect.
Accompanying drawing explanation
Fig. 1 is shown that the system block diagram of the optical detector of the present invention.
Fig. 2 is shown that the optical detector of the present invention and operationally realizes magnetic enrichment and separation detection
Schematic diagram.
Fig. 3 is shown that the most integrated Magneto separate of optical detector of the present invention and total internal reflection
Cleaning Principle figure.
Fig. 4 is shown that the system hardware block diagram of the optical detector of the present invention.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with concrete real
Execute example, and referring to the drawings, the present invention is described in further detail.
Embodiment 1
The optical detector based on magnetic enrichment with total internal reflection of the present invention, comprising: automatically controlled pair of magnetic field
Concentration and separation system, Systems for optical inspection and constant-temperature heating system, automatically controlled pair of magnetic field concentration and separation system
For producing magnetic field alternately, to realize the bi-directional drive of magnetic particle on chip and enrichment；Optics is examined
Examining system uses total internal reflection detection technique, to realize detection and the analyzing and processing of optical imagery；Constant temperature
Heating system includes temperature sensor and heating plate, for controlling to detect the temperature of environment.
Automatically controlled pair of magnetic field concentration and separation system includes: power supply, electromagnetic control circuit program and two phases
Same small size magnetic post, produces magnetic field alternately by alternately power up/down, to realize magnetic grain on chip
The bi-directional drive of son and enrichment.
Wherein, electromagnetic control circuit program can realize adding to two magnetic posts respectively according to the frequency set
Electricity produces magnetic and de-electrifying elimination magnetic, thus realizes separation and the enrichment of magnetic particle.
Systems for optical inspection uses total internal reflection detection technique, and it includes launching light path and detection light path two
Part.Launching light path and be mainly light source emitting module, it comprises control circuit for light source plate and program, LED
Or parallel laser light source.Wherein control circuit for light source plate and program can be to LED or laser
Intensity is set；LED current is revised by RS485 interface by key-press input or host computer
Numerical value, and then change the brightness of light source.
LED or parallel laser light source include the parts such as beam expanding lens and diaphragm, the angle of divergence of light source, light
The parameters such as speckle can be regulated by modes such as collimation, diaphragms, forms a branch of uniform directional light.
Parallel laser light source uses red collimated light beam.Detection light path includes optical detecting module, image
Identify software, microlens.Wherein, optical detecting module uses CCD camera, and pixel is 100
More than ten thousand pixels；Simultaneously need to coordinate microlens to carry out the shooting of image.Image recognition software uses
The software such as MATLAB or C realize the display to image, storage and the contrast of gray scale discriminatory analysis and
Dimensional analysis.
Constant-temperature heating system controls the temperature of detection environment at 37 DEG C by temperature sensor and heating plate
Temperature constant state, so that immunoreation is carried out under specified temp environment, eliminates the Low Response that temperature causes
Different error.
Temperature sensor obtains conversion zone temperature data in real time, then uses pid algorithm to calculate and adds
Backing voltage exporting change amount, and by the heating voltage after the output adjustment of heating plate drive circuit, it is achieved
Accurate control to regional temperature.
When detection, concrete operations are: add chip, preheater apparatus, arrange the conjunction of magnetic field and light source
Suitable numerical value, adds sample, and instrument automatically begins to enter detection pattern, detects complete, image taking,
Software identification image, and according to pre-calibration value, calculate the testing concentration in sample, show numerical value.
Specifically can be found in shown in Fig. 1 and Fig. 4, Fig. 1 is the system frame of the optical detector of the present invention
Figure, Fig. 4 is hardware block diagram.Operationally, optical detector is mainly by detection chip 1, control electricity
Road 2, light source 3, solenoid 4-1 and 4-2, detection photographic head 5, host computer 6 etc. are constituted.
After start, preheat self-starting, complete instrument self checking work, drive including circuit driven by electromagnetic means 2-1
Galvanic electricity magnetic coil 4-1 and 4-1 carries out field drives scanning, light source driving circuit 2-2 carries out light source 3
Brightness calibration, temperature control circuit 2-3 carry out region constant temperature self-inspection, display lamp 2-4 prompting, control circuit
2 complete the work such as parameter setting by communication interface 7 with host computer 6 and photographic head 5.
When detecting, detection chip 1 is placed on instrument chip carrier, region thermostatic control electricity
Road 2-3 drives heating plate that detection chip 1 is warming up to 37 DEG C, and dropping sample (containing mAlb, is urinated micro-
Amount albumin) 1 drop to the sample cell 1-2 of detection chip 1, under siphonage, sample enters through passage 1-3
Enter reaction tank 1-1, it is achieved auto injection.
There is optical detector by chip channel 1-3 position, catch the sample change by optical signal,
And detection trigger, it is achieved instrument detection self-starting.
After detection starts, the sample determinand entering reaction tank 1-1 is received with the anti-mAlb-in reaction tank
Rice magnetic particle is combined reaction, forms mAlb-anti-mAlb-nanomagnetic particle complex.Control electricity
Automatically controlled pair of magnetic field concentration and separation pattern is opened by the electromagnetic coil driving circuit 2-1 in road 2, alternately gives
Upper solenoid 4-1 and lower solenoid 4-2 powers on, and forms the magnetic field that direction converts alternatively up and down.
Under the effect in conversion magnetic field, anti-mAlb-nanomagnetic particle moves up and down in reaction tank 1-1, increases
Add and the contacting of mAlb in sample, improve reaction bonded speed, the response time can be shortened.
MAlb-anti-mAlb-nanomagnetic particle complex, by the action of a magnetic field alternately, can collide anti-
Answer bottom the 1-1 of pond, will combine with the pairing antibody fixedly secured bottom reaction tank 1-1, formed
Antibody 2-mAlb-anti-mAlb-nanomagnetic particle complex, so that magnetic particle is fixed on reaction tank 1-1
On the surface of bottom.
After reacting three minutes, mAlb-anti-mAlb-nanomagnetic particle complex is fixed at the bottom of reaction tank
Portion, quantity tends towards stability, and stop transformation magnetic direction, and upper solenoid 4-1 is kept powered on, magnetic field
Direction maintains upwards.Under the effect of upwards magnetic field force, unnecessary loose magnetic particle will be inhaled
On the upside of reaction tank 1-1.
The mAlb-anti-mAlb-nanomagnetic particle complex being combined with determinand is by dual anti-combination
Being fixed on the downside of reaction tank 1-1, its density is directly proportional to the concentration of determinand.Owing to magnetic particle is fixed
Bottom reaction tank 1-1, total internal reflection interface bottom reaction tank 1-1 will be destroyed.Original light source 3
Launch light beam interface bottom reaction tank 1-1 to be totally reflected, now depositing due to fixing magnetic force
, reflection light is by weakened, and reflective light intensity exists inverse relation with the quantity of fixing magnetic particle.
After upwards the action of a magnetic field 10 seconds, loose magnetic particle is drawn to upper strata substantially.To take the photograph
As a 5 unlatching image acquisition, catch the magnetic particle total internal reflection images bottom shooting reaction tank 1-1,
And send to host computer 6 and carry out software identification and analysis, it is thus achieved that reflective light intensity information, further
Calculate the concentration of mAlb in sample, and show testing result.
Fig. 2 and Fig. 3 is that the optical detector of the present invention operationally realizes magnetic enrichment and separation detection
Schematic diagram.First by two kinds of reactants, (such as antibody 1 combines magnetic particle and forms compound magnetic microparticles admittedly
The top being scheduled in chip；Antibody 2 is fixed on the lower section in chip) it is separately fixed at table in chip
On facial film, being subsequently adding testing sample, the antigen in sample occurs with the compound magnetic microparticles on chip
Association reaction；Power up to respectively upper and lower two magnetic coils by automatically controlled pair of magnetic field according to certain frequency, produce
The magnetic field of down conversion in life, during lower the action of a magnetic field, the compound magnetic microparticles of conjugated antigen accelerates to chip
Lower section is mobile, is combined with antibody 2, forms firm conjugate, be attached to beneath chips.Upper magnetic field
During effect, unconjugated compound magnetic microparticles is attracted to above chip.Magnetic field is executed the most repeatedly up and down
Adding, accelerate antigen and be combined the combination of magnetic microparticles and lower section antibody and separate, antibody-magnetic particle exists
Reaction tank moves up and down, adds and the contacting of test substance in sample, improve reaction bonded speed
Degree, greatly reduces the response time.The action of a magnetic field it is applied with again, by unconjugated many after reaction fully
Remaining compound magnetic microparticles is attracted to above chip, and the antigen of reaction-compound magnetic microparticles is then by anti-
Body 2 combination stays beneath chips, forms magnetic microparticles and assembles, owing to antigen concentration is different, by
Magnetic microparticles formed accumulation regions gray value can change therewith, the concentration of determinand be subsequently fixed at
The nanomagnetic particle density of reaction tank bottom side is directly proportional.The converging parallel light that light source sends is by chip
After optical angle face enters chip, the magnetic particle of gathering causes the variations in refractive index of skin covering of the surface, total reflection
Rear light is irradiated on CCD face battle array or other optical element receptor, record in real time whole instead
Answer the signal intensity of process, it is possible to ccd image or other light of the different gray scales collected
Learning image, use corresponding software processes, analog-to-digital conversion is for drawing light intensity data curve, through demarcating
After, it is thus achieved that the parameters such as the concentration of measured object.
Particular embodiments described above, is carried out the purpose of the present invention, technical scheme and beneficial effect
Further describe it should be understood that the foregoing is only the specific embodiment of the present invention,
Be not limited to the present invention, all within the spirit and principles in the present invention, any amendment of being made,
Equivalent, improvement etc., should be included within the scope of the present invention.Such as: magnetic coil
Outward appearance kind, the kind of optics, different light path preparation methods etc..
Claims (10)
1. an optical detector based on magnetic enrichment with total internal reflection, it is characterised in that comprising:
The automatically controlled pair of magnetic field concentration and separation system, Systems for optical inspection and constant-temperature heating system, described automatically controlled pair of magnetic
Field concentration and separation system is for producing magnetic field alternately, to realize the bi-directional drive of magnetic particle on chip
And enrichment；Described Systems for optical inspection uses total internal reflection detection technique, to realize the inspection of optical imagery
Survey and analyzing and processing；Described constant-temperature heating system includes temperature sensor and heating plate, is used for controlling inspection
Survey the temperature of environment.
The most according to claim 1 based on magnetic enrichment and the optical detector of total internal reflection, its
Being characterised by, described automatically controlled pair of magnetic field concentration and separation system is by being placed on the upper and lower both sides of detection chip
Magnetic post or magnetic sheet, alternately power up/down produces magnetic field alternately, to realize magnetic particle on chip
Bi-directional drive and enrichment；Preferably, described automatically controlled pair of magnetic field concentration and separation system includes power supply, electricity
Control circuit program and two identical magnetic posts.
The most according to claim 2 based on magnetic enrichment and the optical detector of total internal reflection, its
Being characterised by, wherein, described electromagnetic control circuit program realizes according to the frequency set respectively to two
Magnetic post powers up generation magnetic and de-electrifying elimination magnetic, thus realizes separation and the enrichment of magnetic particle；
Preferably, the time range powered up described in is 0.1～30 second；Cycle period is 0～100 cycle；
It is 0.5～100s for last the time of staying of magnetic separate particles；The intensity powered up is 0.1～50V；
The time range powered up described in it is highly preferred that is 2～10 seconds；Cycle period is 5～20 cycles；
It is 5～20s for last the time of staying of magnetic separate particles.
The most according to claim 1 based on magnetic enrichment and the optical detector of total internal reflection, its
Be characterised by, described Systems for optical inspection use total internal reflection detection technique, it include launch light path and
Detection light path two parts.
The most according to claim 4 based on magnetic enrichment and the optical detector of total internal reflection, its
Being characterised by, wherein, described transmitting light path is light source emitting module, and it comprises control circuit for light source plate
And program, LED or parallel laser light source.
The most according to claim 5 based on magnetic enrichment and the optical detector of total internal reflection, its
Being characterised by, the intensity of LED or laser can be carried out by described control circuit for light source plate and program
Set, wherein electric current adjusting range preferably 0～1mA.
The most according to claim 5 based on magnetic enrichment and the optical detector of total internal reflection, its
Being characterised by, described parallel laser light source uses red collimated light beam, wavelength preferably 600～650nm.
The most according to claim 4 based on magnetic enrichment and the optical detector of total internal reflection, its
Being characterised by, wherein, described detection light path includes optical detecting module, image recognition software, micro-
Camera lens or telecentric lens.
The most according to claim 8 based on magnetic enrichment and the optical detector of total internal reflection, its
Being characterised by, described optical detecting module uses CCD, CMOS, light emitting diode or photomultiplier transit
Pipe carries out optical imagery detection, and wherein the pixel of preferred CCD camera, more preferably CCD camera exists
More than 1000000 pixels；
Described image recognition software uses MATLAB or C software to realize the display to image, storage
With the contrast of gray scale discriminatory analysis and dimensional analysis.
The most according to claim 1 based on magnetic enrichment and the optical detector of total internal reflection, its
Being characterised by, described constant-temperature heating system controls the temperature of detection environment by temperature sensor and heating plate
Degree, is preferably controlled in 37 DEG C of temperature constant states.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106596473A (en) * | 2016-10-27 | 2017-04-26 | 中国科学院电子学研究所 | Optic detector based on electric control magnet enrichment-separation and total internal reflection magnetic imaging |
| CN106994370A (en) * | 2017-05-23 | 2017-08-01 | 中国科学院电子学研究所 | Micro-fluidic chip based on magnetic cup compound particle |
| CN112697704A (en) * | 2019-10-23 | 2021-04-23 | 广州市微米生物科技有限公司 | Color recognition module and in-vitro diagnosis equipment thereof |
Citations (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5907401A (en) * | 1997-09-03 | 1999-05-25 | The United States Of America As Represented By The Secretary Of The Army | Device and method for performing an optical hall test |
| WO2010035174A1 (en) * | 2008-09-26 | 2010-04-01 | Koninklijke Philips Electronics N.V. | Magnetic-label sensor and cartridge |
| CN101754811A (en) * | 2007-07-09 | 2010-06-23 | 皇家飞利浦电子股份有限公司 | Microelectronic sensor device with magnetic field generator and carrier |
| CN101842690A (en) * | 2007-10-29 | 2010-09-22 | 皇家飞利浦电子股份有限公司 | Frustrated total internal reflection biosensor cartridge |
| CN101868704A (en) * | 2007-11-21 | 2010-10-20 | 皇家飞利浦电子股份有限公司 | Temperature sensor and the biology sensor that uses it |
| CN101903758A (en) * | 2007-12-20 | 2010-12-01 | 皇家飞利浦电子股份有限公司 | Be used for the microelectronic sensor device that target particles detects |
| CN101903760A (en) * | 2007-12-20 | 2010-12-01 | 皇家飞利浦电子股份有限公司 | Positioning of magnetic coils in a sensor device |
| CN101925817A (en) * | 2008-01-22 | 2010-12-22 | 皇家飞利浦电子股份有限公司 | Detection of target components with help of indicator particles |
| CN101932937A (en) * | 2007-12-04 | 2010-12-29 | 皇家飞利浦电子股份有限公司 | Method of measuring molecules in a fluid using label particles |
| CN101939646A (en) * | 2008-02-06 | 2011-01-05 | 皇家飞利浦电子股份有限公司 | Magnetic bead actuation using feedback for ftir biosensor |
| CN102227625A (en) * | 2008-12-02 | 2011-10-26 | 皇家飞利浦电子股份有限公司 | Sensor device for detecting target particles by frustrated total internal reflection |
| CN102472749A (en) * | 2009-06-30 | 2012-05-23 | 皇家飞利浦电子股份有限公司 | Magnetic sensor device, method of operating such a device and sample |
| US20120262565A1 (en) * | 2009-09-28 | 2012-10-18 | Koninklijke Philips Electronics N.V. | Sensor device with imaging optics |
| EP2581744A1 (en) * | 2011-10-14 | 2013-04-17 | Koninklijke Philips Electronics N.V. | Method for detection of coagulation activity and biomarkers |
| CN103890588A (en) * | 2011-10-20 | 2014-06-25 | 皇家飞利浦有限公司 | Magnetic particle detection with incubation period |
| CN103907012A (en) * | 2011-11-03 | 2014-07-02 | 皇家飞利浦有限公司 | Detection of surface-bound magnetic particles |
| CN104764724A (en) * | 2006-12-12 | 2015-07-08 | 皇家飞利浦电子股份有限公司 | Microelectronic sensor for detection of label particles |
| CN105015200A (en) * | 2015-08-12 | 2015-11-04 | 中国科学院电子学研究所 | Optical microfluidics chip for fixing monoclonal antibody modified layer based on nanometer seal |
| CN105044329A (en) * | 2015-08-12 | 2015-11-11 | 中国科学院电子学研究所 | Method of detecting pathogeny based on optical microfluidic chip of magnetic microparticles |
| CN205157567U (en) * | 2015-08-12 | 2016-04-13 | 中国科学院电子学研究所 | Optics micro -fluidic chip who has passageway based on magnetic particle |
-
2016
- 2016-04-26 CN CN201610266441.5A patent/CN105929149B/en active Active
Patent Citations (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5907401A (en) * | 1997-09-03 | 1999-05-25 | The United States Of America As Represented By The Secretary Of The Army | Device and method for performing an optical hall test |
| CN104764724A (en) * | 2006-12-12 | 2015-07-08 | 皇家飞利浦电子股份有限公司 | Microelectronic sensor for detection of label particles |
| CN101754811A (en) * | 2007-07-09 | 2010-06-23 | 皇家飞利浦电子股份有限公司 | Microelectronic sensor device with magnetic field generator and carrier |
| CN101842690A (en) * | 2007-10-29 | 2010-09-22 | 皇家飞利浦电子股份有限公司 | Frustrated total internal reflection biosensor cartridge |
| CN101868704A (en) * | 2007-11-21 | 2010-10-20 | 皇家飞利浦电子股份有限公司 | Temperature sensor and the biology sensor that uses it |
| CN101932937A (en) * | 2007-12-04 | 2010-12-29 | 皇家飞利浦电子股份有限公司 | Method of measuring molecules in a fluid using label particles |
| CN101903758A (en) * | 2007-12-20 | 2010-12-01 | 皇家飞利浦电子股份有限公司 | Be used for the microelectronic sensor device that target particles detects |
| CN101903760A (en) * | 2007-12-20 | 2010-12-01 | 皇家飞利浦电子股份有限公司 | Positioning of magnetic coils in a sensor device |
| CN101925817A (en) * | 2008-01-22 | 2010-12-22 | 皇家飞利浦电子股份有限公司 | Detection of target components with help of indicator particles |
| CN101939646A (en) * | 2008-02-06 | 2011-01-05 | 皇家飞利浦电子股份有限公司 | Magnetic bead actuation using feedback for ftir biosensor |
| WO2010035174A1 (en) * | 2008-09-26 | 2010-04-01 | Koninklijke Philips Electronics N.V. | Magnetic-label sensor and cartridge |
| CN102227625A (en) * | 2008-12-02 | 2011-10-26 | 皇家飞利浦电子股份有限公司 | Sensor device for detecting target particles by frustrated total internal reflection |
| CN102472749A (en) * | 2009-06-30 | 2012-05-23 | 皇家飞利浦电子股份有限公司 | Magnetic sensor device, method of operating such a device and sample |
| US20120262565A1 (en) * | 2009-09-28 | 2012-10-18 | Koninklijke Philips Electronics N.V. | Sensor device with imaging optics |
| EP2581744A1 (en) * | 2011-10-14 | 2013-04-17 | Koninklijke Philips Electronics N.V. | Method for detection of coagulation activity and biomarkers |
| CN103890588A (en) * | 2011-10-20 | 2014-06-25 | 皇家飞利浦有限公司 | Magnetic particle detection with incubation period |
| CN103907012A (en) * | 2011-11-03 | 2014-07-02 | 皇家飞利浦有限公司 | Detection of surface-bound magnetic particles |
| CN105015200A (en) * | 2015-08-12 | 2015-11-04 | 中国科学院电子学研究所 | Optical microfluidics chip for fixing monoclonal antibody modified layer based on nanometer seal |
| CN105044329A (en) * | 2015-08-12 | 2015-11-11 | 中国科学院电子学研究所 | Method of detecting pathogeny based on optical microfluidic chip of magnetic microparticles |
| CN205157567U (en) * | 2015-08-12 | 2016-04-13 | 中国科学院电子学研究所 | Optics micro -fluidic chip who has passageway based on magnetic particle |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106596473A (en) * | 2016-10-27 | 2017-04-26 | 中国科学院电子学研究所 | Optic detector based on electric control magnet enrichment-separation and total internal reflection magnetic imaging |
| CN106994370A (en) * | 2017-05-23 | 2017-08-01 | 中国科学院电子学研究所 | Micro-fluidic chip based on magnetic cup compound particle |
| CN106994370B (en) * | 2017-05-23 | 2019-07-23 | 中国科学院电子学研究所 | Micro-fluidic chip based on magnetic cup compound particle |
| CN112697704A (en) * | 2019-10-23 | 2021-04-23 | 广州市微米生物科技有限公司 | Color recognition module and in-vitro diagnosis equipment thereof |
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