CN105925697B - A kind of Pseudococcidae insect genes barcode detection kit and its detection method - Google Patents

A kind of Pseudococcidae insect genes barcode detection kit and its detection method Download PDF

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CN105925697B
CN105925697B CN201610381928.8A CN201610381928A CN105925697B CN 105925697 B CN105925697 B CN 105925697B CN 201610381928 A CN201610381928 A CN 201610381928A CN 105925697 B CN105925697 B CN 105925697B
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郑斯竹
杨晓军
詹国辉
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SUZHOU CITY INVASIVE PEST PREVENTION AND CONTROL TECHNOLOGY CENTER
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Abstract

The invention discloses a kind of Pseudococcidae insect genes barcode detection technologies, can quickly detect 21 kinds of mealybugs, including at least primer PseJ/PseN reagents and genetic barcode sequence disk more than 20 dosages；Primer PseJ sequence: 5'-CCTTCAACTAATCATAAAAATATNAG-3', primer PseN sequence: 5'-TAAACTTCT GGATGTCCAAAAAATCA-3'；The genetic barcode sequence has taken inventory 93 standard sequence of barcodes, and detailed sequence is shown in SEQ ID NO.1 to SEQ ID NO.93.Pseudococcidae insect genes barcode detection technology of the invention is a kind of efficient, accurate, convenient and fast Pseudococcidae insect molecular detection technology, can distinguish 21 kinds of castes of Pseudococcidae in molecular level, be able to satisfy use demand.

Description

A kind of Pseudococcidae insect genes barcode detection kit and its detection method
Technical field
The present invention relates to a kind of PCR detection kit and its detection method more particularly to a kind of Pseudococcidae insect genes items Shape code detection kit and its detection method, belong to technical field of plant quarantine.
Background technique
Mealybug is the plant-feed insect of a kind of distribution extensively, more than host, in addition to small part is resource insect, mostly in agriculture and forestry The universal and serious pest (Ben-Dov, 2002) of harm occurs.The adult and nymph of the monoid are mainly tender in the children of host plant Position feeding harm, causes growth potential weak；Also pathogenic microorganism, which can be propagated, leads to the generation of plant disease large area；In addition worm The honeydew of body discharge often leads to the generation of dark mildew, photosynthesis is influenced, to the ecology influence of agriculture and forestry production, urban landscape etc. It is huge.
For a long time, the operability and accuracy of the Pseudococcidae sort research based on morphological feature are constantly subjected to query.Powder The a red-spotted lizard bodily form is smaller or small, the rarely seen male worm of some types, limited for the morphological feature that compares；The Individual Size of mealybug, just The typoiogical classification feature that staple length is short, the presence or absence of transparent apertures etc. are important is unstable, is easily affected by and changes (Qi Xiaofeng, 2002)；Traditional coccid typoiogical classification is more demanding to professional standing and experience simultaneously, only systematicalian or warp The personnel for crossing special training could effectively carry out this work；In addition, traditional Morphology of entomology identification method must with completely at Worm is difficult to carry out by morphological feature fast and effective as Senile Mouse object, nymph, ovum with situations such as incomplete polypide Ground identification (Kondo, 2008；Utsugi, 2011).
In recent years, insect molecular biology develops rapidly, in the application of two aspects of Molecular Identification and phylogeny It is relatively broad, by study insect structural nucleic acid molecule seek affiliation between each monoid and evolutionary relationship (Fr é zal, 2008, Zheng Sizhu, 2015).Using Protocols in Molecular Biology, genetic structure in Natural Population, gene flow can be effectively disclosed The problems such as dynamic and selection index system, new technology and methods (Wei Yihan, 2105) is provided for the taxonomic identification of mealybug.Especially DNA The appearance of bar codes technique, more and more research staff joined DNA bar code technical research ranks, and the field is made to become life One of the hot spot of object research (Hebert, 2006).He Yanbiao etc. (2007) is to ocean stern line mealybug, citrus stern line mealybug, nothing Flowers and fruits stern line mealybugPlanococcus ficusDeng 3 kinds of sibling species mealybugs mtDNA COI sequence and 18S and 28S gene order carries out research discovery coccid COI gene order degree of variation and is higher than other genes；Park etc. (2011) passes through The forward primer for redesigning COI gene order, has carried out the study on the efficiency of species identification to Diaspididae and Pseudococcidae, discloses Characteristic (the Deng of scale insect DNA bar code sequence nucleotide compositionet al., 2012)；Saccaggi etc. (2008) It is analyzed successfully by mtDNA COI gene sequence characteristic by grape silk floss mealybugPlanococcus ficus, citrus stern line mealybug and Long tailed mealybugPseudococcus longispinusIt is distinguished.But these data are still far not achieved solves Pseudococcidae kind extensively The problem of class precise Identification.
DNA bar code technology is expanded using one section or a few segment standards, easily, interspecific difference is noticeably greater than intraspecies variation DNA fragmentation identify the new technologies of species, which is proposed by Canadian scholar Hebert earliest.With traditional taxonomic identification Technology is compared, and DNA bar code technology has the advantages that many incomparable: (1) it is easy to operate, species taxonomy identification knowledge is lacked Weary people can also identify species by standard technique process；(2) not by the limit of soil individual development and morphological feature System, as long as there are complete target DNA fragments can meet qualification requirement for individual；(3) technology can be used as traditional taxonomy Supplementary means, help traditional classification scholar to correct previous classification conclusion, solve the insurmountable problem of typoiogical classification.Exactly Since DNA bar code technology has above and other lot of advantages, more and more research staff joined DNA bar code technology Ranks are studied, the field is made to become one of the hot spot of biological study (Hebert, 2006).But the gene bar code about mealybug Fast Detection Technique research, domestic and international relevant system report are still few.Therefore, accurately and reliably mealybug bar code is established to examine Survey technology not only has great importance to China's quarantine departments, even more the supplement to China's genetic resources, especially mealybug evil The supplement of worm resource can make certain contribution in terms of fighting for limited resource for China, while also reflect for the classification of mealybug New method is provided surely.
Summary of the invention
It is an object of the invention to solve above-mentioned technical problem, a kind of Pseudococcidae insect genes bar code inspection is provided Test agent box and its detection method.
The purpose of the present invention is achieved through the following technical solutions:
A kind of Pseudococcidae insect genes barcode detection kit includes at least a genetic barcode sequence disk and 20 use More than amount primer PseJ and primer PseN；The primer PseJ and primer PseN Serial No.,
PseJ:5'- CCTTCAACTAATCATAAAAATATNAG -3'；
PseN:5'-TAAACTTCTGGATGTCCAAAAAATCA-3；
There are 93 standard sequence of barcodes, the standard sequence of barcodes such as SEQ ID in the genetic barcode sequence disk Shown in NO.1 to SEQ ID NO.93.
Preferably, the kit further includes the following reagent of 20 dosages or more: Taq buffer, MgCl2, dNTP Mix, Taq DNA polymerase, ddH2O, DNA standard sample.
Preferably, a kind of detection method of Pseudococcidae insect genes barcode detection kit, is carried out with the kit Amplification the following steps are included:
The preparation of S1, DNA profiling: examination is extracted using GenMagBio zooblast tissue/cell genomic DNA paramagnetic particle method Agent box extracts sample to be tested；
The amplification of S2, DNA bar code sequence: using the DNA test sample in S1 as template, using primer PseJ and primer PseN carries out PCR amplification, the primer PseJ and primer PseN Serial No.,
PseJ:5'- CCTTCAACTAATCATAAAAATATNAG -3'；
PseN:5'-TAAACTTCTGGATGTCCAAAAAATCA-3；The PCR reaction system uses standards system, overall Product is 50 μ L or 25 μ L；
The PCR reaction condition: 95 DEG C of initial denaturation 4min；35 circulations: 94 DEG C of deformation 1min, 48 DEG C of annealing 1min, 72 DEG C extend 90s；Last 72 DEG C of extensions 4min, 4 DEG C of preservations；
S3,8 μ L samples to be tested of mixing and 2 μ L 6 × Glycerol DNA sample-loading buffer loadings, with DNA Marker DL100 is molecular weight marker, at room temperature 120 V of constant pressure, with the EB dye of 3% agarose gel electrophoresis 45min, 0.5% μm of ol/L Color, gel imaging system test sample, photographic analysis；
S4, PCR result judgement:
8 μ L PCR products and 2 μ L 6 × Glycerol DNA sample-loading buffer loadings are mixed, with DNA Marker DL100 is molecular weight marker, at room temperature 120 V of constant pressure, and with 3 % agarose gel electrophoresis 45 minutes, the EB of 0.5% μm of ol/L contaminated Color, gel imaging system test sample, takes pictures；Photo shows that mealybug has with standard DNA sample in the position 700bp and clearly expands Band, as target stripe；
The sequencing of S5, RCR product obtains gene order；
S6, Pseudococcidae insect genes compare
The sequence that mealybug to be measured is sequenced is compared with genetic barcode sequence disk Plays gene sequence of barcodes, If alignment similarity 100%, assert it for the type；It up to 100%, is then carried out when not with any standard bar code sequence similarity The comparison of amino acid genetic distance determines whether in target zone.
Preferably, the PCR reaction system is 50 μ L, wherein containing KCl, 2mmol/L containing 5 μ 10 × Taq of L buffer MgCl2, 200 μm of ol/L dNTP mix, 2U Taq DNA polymerase, 3 μ L DNA profilings, each 1 μm of ol/L of primer；
The PCR reaction system is 25 μ L, wherein 2 μ L of template DNA, contains Mg2+10 × reaction buffer, 2.5 μ L, 2 μ L, 5pmol/L upstream primer of dNTPs 0.2mmol/L and each 1 μ L of downstream primer, 1.0 U Taq archaeal dna polymerase, 0.2 μ L, Sterile water is mended to 25 μ L.
Preferably, the sample in the S1 is the clean mealybug of pineapple, Lee's Bi Lishi ash mealybug, grey mealybug belong to a kind ofDysmicoccus neobrevipes, duckweed silk floss mealybug, Phenacoccus it is a kind ofPhenacoccus avenae, Phenacoccus it is a kind ofPhenacoccus parvus, short-tube lycoris silk floss mealybug, Japan silk floss mealybug, tangerine stern line mealybug, stern line mealybug belong to a kind ofPlanococcus ficus, Japanese stern line mealybug, stern line mealybug belong to a kind ofPlanococcus lilacinus, stern line mealybug belong to a kind ofPlanococcus minor, stern line mealybug belong to a kind ofPlanococcus vovae, mealybug belong to a kind ofPseudococcus baliteus, sugarcane root mealybug, Kang Shi mealybug, mealybug belong to a kind ofPseudococcus cryptus, Jack Bei Ershi mealybug, quasi- length Tail mealybug, mealybug belong to a kind ofPseudococcus viburni。
Preferably, the S6 Pseudococcidae insect genes comparison result includes the following steps,
S61, when obtained sequence and standard gene sequence of barcodes similarity 100%, then assert it for the type；
S62, when not with any standard bar code sequence similarity up to 100%, then by mealybug gene order to be measured press " no ridge Vertebrate codon " is translated as amino acid, uses " P-distance with amino acid sequence corresponding to standard gene sequence of barcodes Model " calculates genetic distance, if the standard gene bar code type with unknown type mealybug gene order genetic distance less than 1%, i.e., For targeted species；If genetic distance is both greater than 1%, determine unknown mealybug type not within the scope of this 21 kinds of mealybugs.
The beneficial effects are mainly reflected as follows: (1) target gene short, reduces the demand tested to specimen quality, Amplification efficiency is high；(2) inter-species variation is big and intraspecific variablity is small, to distinguish different plant species；Comprising enough phyletic evolution information with Position of the species in categorizing system is positioned, use demand is able to satisfy.(3) easy to operate, lack of knowledge is identified to species taxonomy People Pseudococcidae species can also be identified by standard technique process；(4) not by soil individual development and morphological feature Limitation, as long as there are complete target DNA fragments can meet qualification requirement for individual.
Detailed description of the invention
Technical scheme of the present invention is further explained with reference to the accompanying drawing:
Fig. 1: primer PseJ/PseN amplification of Japan silk floss mealybug, three kinds of short-tube lycoris silk floss mealybug, Kang Shi mealybug mealybug samples is tied Fruit figure.
Fig. 2: the sample amplification band schematic diagram in embodiment.
Specific embodiment
Present invention discloses a kind of Pseudococcidae insect genes barcode detection kit and its detection method, this method can be high It imitates, is accurate, convenient and fast Pseudococcidae insect molecular detection technology, can distinguish 21 kinds of castes of Pseudococcidae in molecular level, energy It satisfies the use demand.
A kind of Pseudococcidae insect genes barcode detection kit, can quickly detect 21 kinds of mealybugs, and the mealybug is specific As shown in table 1, the kit includes at least primer PseJ/PseN examination of genetic barcode sequence disk and 20 dosages or more Agent:
Primer PseJ sequence: 5'- CCTTCAACTAATCATAAAAATATNAG -3',
Primer PseN sequence: 5'- TAAACTTCT GGATGTCCAAAAAATCA-3'；
The genetic barcode sequence has taken inventory 93 standard sequence of barcodes, and detailed sequence is SEQ ID NO.1 to SEQ Shown in ID NO.93.
Table 1: mealybug type and title
Serial number Sample title Latin name
1 The clean mealybug of pineapple Dysmicoccus brevipes
2 Lee's Bi Lishi ash mealybug Dysmicoccus lepelleyi
3 Grey mealybug belongs to a kind of Dysmicoccus neobrevipes
4 Duckweed silk floss mealybug Phenacoccus aceris
5 Phenacoccus is a kind of Phenacoccus avenae
6 Phenacoccus is a kind of Phenacoccus parvus
7 Short-tube lycoris silk floss mealybug Phenacoccus solani
8 Japan silk floss mealybug Phenacoccus solenopsis
9 Tangerine stern line mealybug Planococcus citri
10 Stern line mealybug belongs to a kind of Planococcus ficus
11 Japanese stern line mealybug Planococcus kraunhiae
12 Stern line mealybug belongs to a kind of Planococcus lilacinus
13 Stern line mealybug belongs to a kind of Planococcus minor
14 Stern line mealybug belongs to a kind of Planococcus vovae
15 Mealybug belongs to a kind of Pseudococcus baliteus
16 Sugarcane root mealybug Pseudococcus calceolariae
17 Kang Shi mealybug Pseudococcus comstocki
18 Mealybug belongs to a kind of Pseudococcus cryptus
19 Jack's Bei Ershi mealybug Pseudococcus jackbeardsleyi
20 Quasi- long tailed mealybug Pseudococcus longispinus
21 Mealybug belongs to a kind of Pseudococcus viburni
Preferably, the kit further includes the following reagent of 20 dosages or more: Taq buffer, MgCl2, dNTP Mix, Taq DNA polymerase, ddH2O, DNA standard sample.
Lower detection and differentiating method using the kit detailed below:
Six parts of Japan silk floss mealybug, short-tube lycoris silk floss mealybug, Kang Shi mealybug, Callosobruchus maculatus, dermestes maculatus, ceroplastes rubens samples are chosen to make For sample to be tested.Steps are as follows:
1) Genome DNA extraction
The extraction of DNA is carried out using GenMagBio zooblast tissue/cell genomic DNA magnetic bead extracts kit It extracts.Detailed process is as follows:
1-2 insect specimens are directly put into 2mm test tube, soaked in absolute ethyl alcohol, which saves sample and rinsed with sterile distilled water, soaks Bubble 4 ~ 5 times abandons water；Exsiccata sterile distilled water impregnates suck dry moisture after 3h.It is placed in 2mL centrifuge tube, is shaken in MM400 ball milling instrument Mill (30 times/s) 30s, and 180 μ L Lysis Buffer, 20 μ L Proteinase K are added, and concussion mixes, ambient temperature overnight, and 55 DEG C concussion warm bath 3-5h, 12000rpm are centrifuged 10min, take supernatant, and 200 μ L Binding Buffer and the 200 anhydrous second of μ L are added Alcohol mixes well, and 20 μ L magnetic beads are added, and gentle inversion mixes 10min.Centrifuge tube is placed in magnetic frame, inhales and abandons liquid in pipe, retains Magnetic bead, Wash Buffer 500uL are mixed by inversion 2min and are placed in magnetic frame, discard liquid in pipe, and Wash Buffer II is same Sample washes twice, and centrifuge tube is still placed on magnetic frame, is slowly added to Wash Buffer III, removes liquid in pipe after 1min.Add Enter 20 μ L Elution Buffer, the DNA adsorbed on 55 DEG C of water-bath 10min elution magnetic beads, 4 DEG C store for future use.
2) sequence amplification and measurement
PCR reaction system: total volume is 50 μ L, wherein containing 5 μ L 10 × Taq buffer with KCl, 2 mmol/L MgCl2, 200 μm of ol/L dNTP mix, 2 U Taq DNA polymerases, 3 μ L DNA profilings, each 1 μm of ol/L of primer；
The PCR reaction system is 25 μ L, wherein 2 μ L of template DNA, contains Mg2+10 × reaction buffer, 2.5 μ L, 2 μ L, 5pmol/L upstream primer of dNTPs 0.2mmol/L and each 1 μ L of downstream primer, 1.0 U Taq archaeal dna polymerase, 0.2 μ L, Sterile water is mended to 25 μ L.
Reaction condition: 95 DEG C of initial denaturation 4min；35 circulations: 94 DEG C of deformation 1min, 48 DEG C of annealing 1min, 72 DEG C extend 90s；Last 72 DEG C of extensions 4min, 4 DEG C of preservations；
8 μ L samples to be tested and 2 μ L 6 × Glycerol DNA loading Buffer loadings are mixed, with DNA Marker DL100 is molecular weight marker, at room temperature 120 V of constant pressure, with 3% agarose gel electrophoresis, 45 min, 0.5% μm of ol/L EB dyeing, carry out gel imaging system test sample, take pictures, analyze；
Electrophoretogram, as shown in Fig. 2, in figure 1 be Japan silk floss mealybug；2 be short-tube lycoris silk floss mealybug in figure；3 be Kang Shi mealybug in figure； 4 be Callosobruchus maculatus in figure；5 be dermestes maculatus in figure；6 be ceroplastes rubens in figure.
In six samples, three kinds of mealybugs, ceroplastes rubens amplify the band of 700bp size, Callosobruchus maculatus, dermestes maculatus do not expand Increase shaping band, both of exclusion meet reality not within Pseudococcidae insect, and it is accurate to exclude.
The product of band is amplified above using PCR primer as sequencing primer, bidirectional sequencing is carried out, by Suzhou Jin Weizhi Biotechnology Co., Ltd completes.
3) gene compares
Using existing comparison software and computer technology, the sequence results that unknown type mealybug is sequenced and gene Bar code sequence disk Plays gene sequence of barcodes is compared, and concrete outcome is run in the following manner:
A: when sequence and standard gene sequence of barcodes similarity 100%, then assert it for the type；
B: when sequence not with any standard bar code sequence similarity up to 100%, then unknown type mealybug gene order is pressed " invertebrate codon " is translated as amino acid, uses " P-distance model " with the amino acid sequence of standard gene bar code Calculate genetic distance, the standard gene bar code type with unknown type mealybug gene order genetic distance less than 2%, as target Type；If genetic distance is both greater than 1%, illustrate unknown mealybug type not within the scope of this 21 kinds of mealybugs.
Accuracy detection:
5 sequences of gained sequentially input genetic barcode sequence disk and carry out calculating ratio pair, as a result as follows:
1) short-tube lycoris silk floss mealybug sequence and paddy spot mealybug standard bar code gene similarity in sequence disk are 100%, and qualification result is quasi- Really.
2) Japan silk floss mealybug sequence and Japan silk floss mealybug standard bar code gene similarity in sequence disk are 98%, with sequence disk Middle Japan silk floss mealybug standard bar code gene genetic distance 0.2%, regards as Japan silk floss mealybug, qualification result by result confirmative rules Accurately.
3) Kang Shi mealybug sequence and Kang Shi mealybug standard bar code gene similarity in sequence disk are 98% ~ 100%, with sequence disk In Kang Shi mealybug standard bar code gene genetic distance between 0% ~ 0.88%, regard as Kang Shi mealybug by result confirmative rules, Qualification result is accurate.
4) ceroplastes rubens and mealybug standard bar code gene genetic distance in sequence disk are 31.9% ~ 39.7%, are much larger than 2%, press and tie Fruit confirmative rules assert non-21 kinds of mealybugs.
All results are consistent with the specimen types for examination, as a result meet the requirements, show the kit can with precise Identification this 3 Kind mealybug.
Still there are many specific embodiment, all skills formed using equivalent replacement or equivalent transformation by the present invention Art scheme, all falls within the scope of protection of present invention.
NO.1: Dysmicoccus brevipes 1 of SEQ ID
TTTTTTTTATAACTATACCAATTATTATTGGAAGATTAAGTAATTGATTATTACCTTTAATATTAATAT CAAGAGATTTAATATTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATACCTTCATTAATGTTTATATTATTA AATATATTATTAAGAGATAATATTGATACTGGATGAACATTATATCCTCCTTTAATTAATCAAAATTTTTTCACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGTTTATCATCAATTTTTAGTTCAATTAATTTTATTGTTTCTATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATTTAACTTTATATATTTGATCTATTATTATTACTACTATTTTA CTTATTATTTCTATTCCTATTTTATCAAGAGCTATTACTATAATTATTTTAGATAAAAATTTCAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.2:Dysmicoccus brevipes 2
TTTTTTTTATAACTATACCAATTATTATTGGAAGATTAAGTAATTGATTATTACCTTTAATATTAATAT CAAGAGATTTAATATTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATACCTTCATTAATGTTTATATTATTA AATATATTATTAAGAGATAATATTGATACTGGATGAACATTATATCCTCCTTTAATTAATCAAAATTTTTTCACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGTTTATCATCAATTTTTAGTTCAATTAATTTTATTGTTTCTATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATTTAACTTTATATATTTGATCTGTTATTATTACTACTATTTTA CTTATTATTTCTATTCCTATTTTATCAAGAGCTATTACTATAATTATTTTAGATAAAAATTTCAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.3:Dysmicoccus brevipes 3
TTTTTTTTATAACTATACCAATTATTATTGGAAGATTAAGTAATTGATTATTACCTTTAATATTAATAT CAAGAGATTTAATATTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATACCTTCCTTAATATTTATATTATTA AATATATTATTAAGAGATAATATTGATACTGGATGAACATTATATCCTCCTCTAATTAATCAAAATTTTTTTACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGTTTATCATCAATTTTTAGTTCAATTAATTTTATTGTTTCTATTT TTATTATTAATAATAATAATTTTTTTATTAATGATTTAACTTTATATATTTGATCTATTATTATTACTACTATTTTA CTTATTATTTCTATTCCTATTTTATCAAGAGCTATTACTATAATTATTTTAGATAAAAATTTTAATATAAATTTCTT TAATCCTTTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.4:Dysmicoccus brevipes 4
TTTTTTTTATAACTATACCAATTATTATTGGAAGATTAAGTAATTGATTATTACCTTTAATATTAATAT CAAGAGATTTAATATTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATACCTTCATTAATGTTTATATTATTA AATATATTATTAAGAGATAATATTGATACGGGATGAACATTATATCCTCCTTTAATTAATCAAAATTTTTTCACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGTTTATCATCAATTTTTAGTTCAATTAATTTTATTGTTTCTATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATTTAACTTTATATATTTGATCTATTATTATTACTACTATTTTA CTTATTATTTCTATTCCTATTTTATCAAGAGCTATTACTATAATTATTTTAGATAAAAATTTCAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.5:Dysmicoccus brevipes 5
TTTTTTTTATAACTATACCAATTATTATTGGAAGATTAAGTAATTGATTATTACCTTTAATATTAATAT CAAGAGATTTAATATTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATACCTTCATTAATGTTTATATTATTA AATATATTATTAAGAGATAATATTGATACTGGATGAACATTATATCCTCCTTTAATTAATCAAAATTTTTTCACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGTTTATCATCAATTTTTAGTTCAATTAATTTTATTGTTTCTATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATTTAACTTTATATATTTGATCTATTATTATTACTACTATTTTA CTTATTATTTCTATTCCTATTTTATCAAGAGCTATTACTATAATTATTTTAGATAAAAATTTCAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.6:Dysmicoccus lepelleyi 1
TTTTTTTTATAACTATACCAATCATTATTGGAAGATTAAGTAATTGACTTTTACCTCTAATATTAATAT CATCAGATTTAATTTTTCCACGTTTAAATAATTTTAGATTTTGATTATTAATTCCTTCTTTAATTTTAATAATATTA AATATAATTTTATTAAATAATATTAATACTGGATGAACATTATACCCTCCTTTAATTAATCAAAACTTTATTACATT AAATTTTATTATTTTTTCCCTTCATTTAAATGGTATTTCTTCAATTTTTAGATCAATCAATTTTATTTCATCAATTT TTATTATTAATAATAACAATTTTTTTTTAAATAATATTTCATTATATATTTGATCGATTATTGTTACAACAATTTTA TTAATTATTTCTATTCCAATTTTATCAAGAGCAATTACTATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGTAACCCTATTTTATATCAACATTTATTT
SEQ ID NO.7:Dysmicoccus lepelleyi 2
TTTTTTTTATAACTATACCAATCATTATTGGAAGATTAAGTAATTGACTTTTACCTCTAATATTAATAT CATCAGATTTAATTTTTCCACGTTTAAATAATTTTAGATTTTGATTATTAATTCCTTCTTTAATTTTAATAATATTA AATATAATTTTATTAAATAATATTAATACTGGATGAACATTATACCCTCCTTTAATTAATCAAAACTTTATTACATT AAATTTTATTATTTTTTCCCTTCATTTAAATGGTATTTCTTCAATTTTTAGATCAATCAATTTTATTTCATCAATTT TTATTATTAATAATAACAATTTTTTTTTAAATAATATTTCATTATATATTTGATCAATTATTGTTACAACAATTTTA TTAATTATTTCTATTCCAATTTTATCAAGAGCAATTACTATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGTAACCCTATTTTATATCAACATTTATTT
SEQ ID NO.8:Dysmicoccus lepelleyi 3
TTTTTTTTATAACTATACCAATCATTATTGGAAGATTAAGTAATTGACTTTTACCTCTAATATTAATAT CATCAGATTTAATTTTTCCACGTTTAAATAATTTTAGATTTTGATTATTAATTCCTTCTTTAATTTTAATAATATTA AATATAATTTTATTAAATAATATTAATACTGGATGAACATTATACCCTCCTTTAATTAATCAAAACTTTATTACATT AAATTTTATTATTTTTTCCCTTCATTTAAATGGTATTTCTTCAATTTTTAGATCAATCAATTTTATTTCATCAATTT TTATTATTAATAATAACAATTTTTTTTTAAATAATATTTCATTATATATTTGATCAATTATTGTTACAACAATTTTA TTAATTATTTCTATTCCAATTTTATCAAGAGCAATTACTATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGTAACCCTATTTTATATCAACATTTATTT
SEQ ID NO.9:Dysmicoccus neobrevipes 1
TTTTTTTTATAACAATACCTATTATTATTGGTAGTTTAAGAAATTGATTATTACCTTTAATATTAATAT CTAGAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATTCCTTCTTTAATATTTATAATAATA AATATATTATTAAATAATAATATTAATACTGGATGAACTTTATATCCTCCATTAATTAATCAAAATTTTATTACTTT AAATTTTATTATTTTTTCTCTTCATTTAAATGGAATTTCATCAATTTTTAGATCAATTAATTTTATTGCTTCTATTT TTATTATTAATAATAATAATTTTTTTATTAATAATTTAACTTTATATATTTGATCTATTATTATTACAACATTTCTA TTAATTATTTCAATTCCTATTTTATCTAGAGCTATTACTATAATTATTCTTGATAATAATTTTAATATAAATTTTTT TAATCCTTTAGGTAATGGTAATCCTATTCTTTATCAACATCTATTT
SEQ ID NO.10:Dysmicoccus neobrevipes 2
TTTTTTTTATAACAATACCTATTATTATTGGTAGTTTAAGAAATTGATTATTACCTTTAATATTAATAT CTAGAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATTCCTTCTTTAATATTTATAATAATA AATATATTATTAAATAATAATATTAATACTGGATGAACTTTATATCCTCCATTAATTAATCAAAATTTTATTACTTT AAATTTTATTATTTTTTCTCTTCATTTAAATGGAATTTCATCAATTTTTAGATCAATTAATTTTATTGCTTCTATTT TTATTATTAATAATAATAATTTTTTTATTAATAATTTAACTTTATATATTTGATCTATTATTATTACAACATTTCTA TTAATTATTTCAATTCCTATTTTATCTAGAGCTATTACTATAATTATTCTTGATAATAATTTTAATATAAATTTTTT TAATCCTTTAGGTGATGGTAATCCTATTCTTTATCAACATCTATTT
SEQ ID NO.11:Phenacoccus aceris 1
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAGGAAATTGATTAATTCCTTTAATATTATCAT CTTCAGATTTATTATTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATCCCTTCTCTAATATTAATAATTTTA AATTCATTTATTAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTCTAATTAATCAAAATTTTATTACATT AAATTTTATTATCTTTTCCTTACATTTAAATGGAATTTCATCAATTTTTAGATCTATTAATTTTATTACTTCAATTT TTATAATTAATAATAAAAATTTTTTAACTAAAAATTATTCTTTATATATTTGATCAATTATTGTTACAACAATTTTA CTTATTTCTTCTATTCCTGTTCTTTCAAGAGCAATTACAATAATTATTTTAGATAATAATTTAAATATAAACTTTTT TAATCCTCTAGGAAATGGAAATCCAATTTTATACCAACATTTATTC
SEQ ID NO.12:Phenacoccus aceris 2
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAGGAAATTGACTAATTCCTTTAATATTATCTT CTTCTGATTTACTATTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATTCCTTCTTTAATATTAATAATTTTA AATTCTTTTATTAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTTTAATTAACCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCATCAATTTTTAGATCAATTAATTTTATTACTTCAATTT TTATAATCAATAATAAAAATTTTTTAACTAAAAACTATTCTTTATATATTTGATCAATTATTGTTACAACAATTCTA TTAATTTCATCAATCCCTGTTCTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCATTAGGTAATGGAAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.13:Phenacoccus aceris 3
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAGGAAATTGACTAATTCCTTTAATATTATCTT CTTCTGATTTACTATTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATTCCTTCTTTAATATTAATAATTTTA AATTCTTTTATTAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTTTAATTAACCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCATCAATTTTTAGATCAATTAATTTTATTACTTCAATTT TTATAATCAATAATAAAAATTTTTTAACTAAAAACTATTCTTTATATATTTGATCAATTATTGTTACAACAATTCTA TTAATTTCATCAATCCCTGTTCTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCATTAGGTAATGGAAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.14:Phenacoccus aceris 4
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAGGAAATTGATTAATTCCTTTAATATTATCAT CTTCAGATTTATTATTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATCCCTTCTCTAATATTAATAATTTTA AATTCATTTATTAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTCTAATTAATCAAAATTTTATTACATT AAATTTTATTATCTTTTCCTTACATTTAAATGGAATTTCATCAATTTTTAGATCTATTAATTTTATTACTTCAATTT TTATAATTAATAATAAAAATTTTTTAACTAAAAATTATTCTTTATATATTTGATCAATTATTGTTACAACAATTTTA CTTATTTCTTCTATTCCTGTTCTTTCAAGAGCAATTACAATAATTATTTTAGATAATAATTTAAATATAAACTTTTT TAATCCTCTAGGAAATGGAAATCCAATTTTATACCAACATTTATTC
SEQ ID NO.15:Phenacoccus aceris 5
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAGGAAATTGATTAATTCCTTTAATATTATCAT CTTCAGATTTATTATTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATCCCTTCTCTAATATTAATAATTTTA AATTCATTTATTAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTCTAATTAATCAAAATTTTATTACATT AAATTTTATTATCTTTTCCTTACATTTAAATGGAATTTCATCAATTTTTAGATCTATTAATTTTATTACTTCAATTT TTATAATTAATAATAAAAATTTTTTAACTAAAAATTATTCTTTATATATTTGATCAATTATTGTTACAACAATTTTA CTTATTTCTTCTATTCCTGTTCTTTCAAGAGCAATTACAATAATTATTTTAGATAATAATTTAAATATAAACTTTTT TAATCCTCTAGGAAATGGAAATCCAATTTTATACCAACATTTATTC
SEQ ID NO.16:Phenacoccus aceris 6
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAGGAAATTGACTAATTCCTTTAATATTATCTT CTTCTGATTTACTATTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATTCCTTCTTTAATATTAATAATTTTA AATTCTTTTATTAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTTTAATTAACCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCATCAATTTTTAGATCAATTAATTTTATTACTTCAATTT TTATAATCAATAATAAAAATTTTTTAACTAAAAACTATTCTTTATATATTTGATCAATTATTGTTACAACAATTCTA TTAATTTCATCAATCCCTGTTCTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCATTAGGTAATGGAAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.17:Phenacoccus aceris 7
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAGGAAATTGACTAATTCCTTTAATATTATCTT CTTCTGATTTACTATTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATTCCTTCTTTAATATTAATAATTTTA AATTCTTTTATTAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTTTAATTAACCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCATCAATTTTTAGATCAATTAATTTTATTACTTCAATTT TTATAATCAATAATAAAAATTTTTTAACTAAAAACTATTCTTTATATATTTGATCAATTATTGTTACAACAATTCTA TTAATTTCATCAATCCCTGTTCTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCATTAGGTAATGGAAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.18:Phenacoccus aceris 8
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAGGAAATTGACTAATTCCTTTAATATTATCTT CTTCTGATTTACTATTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATTCCTTCTTTAATATTAATAATTTTA AATTCTTTTATTAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTTTAATTAACCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCATCAATTTTTAGATCAATTAATTTTATTACTTCAATTT TTATAATCAATAATAAAAATTTTTTAACTAAAAACTATTCTTTATATATTTGATCAATTATTGTTACAACAATTCTA TTAATTTCATCAATCCCTGTTCTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCATTAGGTAATGGAAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.19:Phenacoccus avenae
TCTTTTTTATAACTATACCTATCATTATTGGAAGATTAGGAAATTGACTTTTACCTTTAATAATTAGAT CATCAGATTTAATATTTCCACGATTAAATAATTTTAGATTTTGACTATTATTACCTTCTTTAATATTAATGATTTTA AATCTTTTTATTAATAATAACATTAATACAGGATGAACTTTATATCCCCCATTAATTAATCAAAACTTTATTACCTT AAATTTTATTATTTTTTCTCTTCATTTAAATGGTATTTCATCAATTTTTAGATCAATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTTCAAATAATTTCACCCTTTATATTTGATCAATTATTGTTACTACTATTCTT TTAATTATTTCAATTCCTGTTTTATCAAGAGCTATTACTATAATTATTCTAGATAATAATATAAATATAAATTTTTT TAATCCCTTAGGAAATGGAAATCCCATTCTTTATCAACATTTATTT
SEQ ID NO.20:Phenacoccus parvus 1
TTTTCTTTATAACTATACCAATTATTATTGGAAGATTAAGAAATTGACTTTTACCTTTGATAATTTCCT CTTCTGATTTAATATTTCCACGATTAAATAATTTTAGATTTTGATTACTTCTCCCTTCTTTAATATTATTAATTTTA AATATAATATTAAACAATAATATTAATACAGGTTGAACACTTTATCCCCCATTAATTAATCAAAATTTCATTACTTT AAATTTTATTATTTTTTCCCTTCATTTAAATGGGCTATCCTCTATTTTTAGTTCAATTAATTTTATCTCTTCAATTA TAATTATTAACAATAATAAATTTTTTTTAAATAATTTATCATTATATATTTGATCTATTTTAATTACTACTTTTCTT TTAATTGTTTCAATTCCTGTTTTATCAAGGGCTATTACTATAATTATTCTTGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGTAATGGGAACCCGATTTTATATCAACATCTATTT
SEQ ID NO.21:Phenacoccus parvus 2
TTTTCTTTATAACTATACCAATTATTATTGGAAGATTAAGAAATTGACTTTTACCTTTGATAATTTCCT CTTCTGATTTAATATTTCCACGATTAAATAATTTTAGATTTTGATTACTTCTCCCTTCTTTAATATTATTAATTTTA AATATAATATTAAACAATAATATTAATACAGGTTGAACACTTTATCCCCCATTAATTAATCAAAATTTCATTACTTT AAATTTTATTATTTTTTCCCTTCATTTAAATGGGCTATCCTCTATTTTTAGTTCAATTAATTTTATCTCTTCAATTA TAATTATTAACAATAATAAATTTTTTTTAAATAATTTATCATTATATATTTGATCTATTTTAATTACTACTTTTCTT TTAATTGTTTCAATTCCTGTTTTATCAAGGGCTATTACTATAATTATTCTTGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGTAATGGGAACCCGATTTTATATCAACATCTATTT
SEQ ID NO.22:Phenacoccus solani 1
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTTAGAAATTGACTATTACCTTTAATAATCTCAT CTTCAGATTTAATATTCCCTCGATTAAATAATTTTAGATTCTGACTTCTTTTACCTTCTTTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTTTAATTAATCAAAATTTTATTACCTT AAATTTTATTATTTTTTCTTTACATTTAAATGGTTTATCATCAATTTTTAGATCAATTAATTTTATTTCTTCTATTA TAGTAATTAATAATAATAATTTTTTTTTAAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCTATTCCAATTCTTTCTAGAGCAATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCAATTCTTTATCAACATTTATTT
SEQ ID NO.23:Phenacoccus solani 2
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTTAGAAATTGACTATTACCTTTAATAATCTCAT CTTCAGATTTAATATTCCCTCGATTAAATAATTTTAGATTCTGACTTCTTTTACCTTCTTTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTTTAATTAATCAAAATTTTATTACCTT AAATTTTATTATTTTTTCTTTACATTTAAATGGTTTATCATCAATTTTTAGATCAATTAATTTTATTTCTTCTATTA TAGTAATTAATAATAATAATTTTTTTTTAAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCTATTCCAATTCTTTCTAGAGCAATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCAATTCTTTATCAACATTTATTT
SEQ ID NO.24:Phenacoccus solani 3
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTTAGAAATTGACTATTACCTTTAATAATCTCAT CTTCAGATTTAATATTCCCTCGATTAAATAATTTTAGATTCTGACTTCTTTTACCTTCTTTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTTTAATTAATCAAAATTTTATTACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGTTTATCATCAATTTTTAGATCAATTAATTTTATTTCTTCTATTA TAGTAATTAATAATAATAATTTTTTTTTAAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCTATTCCAATTCTTTCTAGAGCAATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TACTCCTTTAGGAAATGGAAATCCAATTCTTTATCAACATTTATTT
SEQ ID NO.25:Phenacoccus solani 4
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTTAGAAATTGACTATTACCTTTAATAATCTCAT CTTCAGATTTAATATTCCCTCGATTAAATAATTTTAGATTCTGACTTCTTTTACCTTCTTTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTTTAATTAATCAAAATTTTATTACCTT AAATTTTATTATTTTTTCTTTACATTTAAATGGTTTATCATCAATTTTTAGATCAATTAATTTTATTTCTTCTATTA TAGTAATTAATAATAATAATTTTTTTTTAAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCTATTCCAATTCTTTCTAGAGCAATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCAATTCTTTATCAACATTTATTT
SEQ ID NO.26:Phenacoccus solani 5
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTTAGAAATTGACTATTACCTTTAATAATCTCAT CTTCAGATTTAATATTCCCTCGATTAAATAATTTTAGATTCTGACTTCTTTTACCTTCTTTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTTTAATTAATCAAAATTTTATTACCTT AAATTTTATTATTTTTTCTTTACATTTAAATGGTTTATCATCAATTTTTAGATCAATTAATTTTATTTCTTCTATTA TAGTAATTAATAATAATAATTTTTTTTTAAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCTATTCCAATTCTTTCTAGAGCAATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCGATTCTTTATCAACATTTATTT
SEQ ID NO.27:Phenacoccus solani 6
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTTAGAAATTGACTATTACCTTTAATAATCTCAT CTTCAGATTTAATATTCCCTCGATTAAATAATTTTAGATTCTGACTTCTTTTACCTTCTTTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATATCCTCCTTTAATTAATCAAAATTTTATTACCTT AAATTTTATTATTTTTTCTTTACATTTAAATGGTTTATCATCAATTTTTAGATCAATTAATTTTATTTCTTCTATTA TAGTAATTAATAATAATAATTTTTTTTTAAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCTATTCCAATTCTTTCTAGAGCAATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCAATTCTTTATCAACATTTATTT
SEQ ID NO.28:Phenacoccus solenopsis 1
TTTTTTTTATGACTATACCTATTATTATCGGAAGATTTAGAAATTGACTTTTACCTTTAATAATTTCAT CATCAGATTTAATATTTCCACGATTAAACAATTTTAGATTTTGACTTCTTTTACCTTCATTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATACCCTCCTTTAATTAATCAAAATTTCATTACTTT AAATTTTATTATTTTCTCTCTTCATTTAAACGGCCTATCTTCAATTTTTAGATCAATCAATTTTATTTCTTCTATTA TAGTTATTAATAATAATAACTTTTTTCTTAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCAATTCCAGTACTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.29:Phenacoccus solenopsis 2
TTTTTTTTATGACTATACCTATTATTATCGGAAGATTTAGAAATTGACTTTTACCTTTAATAATTTCAT CATCAGATTTAATATTTCCACGATTAAACAATTTTAGATTTTGACTTCTTTTACCTTCATTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATACCCTCCTTTAATTAATCAAAATTTCATTACTTT AAATTTTATTATTTTCTCTCTTCATTTAAACGGCCTATCTTCAATTTTTAGATCAATCAATTTTATTTCTTCTATTA TAGTTATTAATAATAATAACTTTTTTCTTAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCAATTCCAGTACTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.30:Phenacoccus solenopsis 3
TTTTTTTTATGACTATACCTATTATTATCGGAAGATTTAGAAATTGACTTTTACCTTTAATAATTTCAT CATCAGATTTAATATTTCCACGATTAAACAATTTTAGATTTTGACTTCTTTTACCTTCATTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATACCCTCCTTTAATTAATCAAAATTTCATTACTTT AAATTTTATTATTTTCTCTCTTCATTTAAACGGCCTATCTTCAATTTTTAGATCAATCAATTTTATTTCTTCTATTA TAGTTATTAATAATAATAACTTTTTTCTTAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCAATTCCAGTACTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.31:Phenacoccus solenopsis 4
TTTTTTTTATGACTATACCTATTATTATCGGAAGATTTAGAAATTGACTTTTACCTTTAATAATTTCAT CATCAGATTTAATATTTCCACGATTAAACAATTTTAGATTTTGACTTCTTTTACCTTCATTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATACCCTCCTTTAATTAATCAAAATTTCATTACTTT AAATTTTATTATTTTCTCTCTTCATTTAAACGGCCTATCTTCAATTTTTAGATCAATCAATTTTATTTCTTCTATTA TAGTTATTAATAATAATAACTTTTTTCTTAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCAATTCCAGTACTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.32:Phenacoccus solenopsis 5
TTTTTTTTATGACTATACCTATTATTATCGGAAGATTTAGAAATTGACTTTTACCTTTAATAATTTCAT CATCAGATTTAATATTTCCACGATTAAACAATTTTAGATTTTGACTTCTTTTACCTTCATTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATACCCTCCTTTAATTAATCAAAATTTCATTACTTT AAATTTTATTATTTTCTCTCTTCATTTAAACGGCCTATCTTCAATTTTTAGATCAATCAATTTTATTTCTTCTATTA TAGTTATTAATAATAATAACTTTTTTCTTAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCAATTCCAGTACTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.33:Phenacoccus solenopsis 6
TTTTTTTTATGACTATACCTATTATTATCGGAAGATTTAGAAATTGACTTTTACCTTTAATAATTTCAT CATCAGATTTAATATTTCCACGATTAAACAATTTTAGATTTTGACTTCTTTTACCTTCATTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATACCCTCCTTTAATTAATCAAAATTTCATTACTTT AAATTTTATTATTTTCTCTCTTCATTTAAACGGCCTATCTTCAATTTTTAGATCAATCAATTTTATTTCTTCTATTA TAGTTATTAATAATAATAACTTTTTTCTTAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCAATTCCAGTACTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.34:Phenacoccus solenopsis 7
TTTTTTTTATGACTATACCTATTATTATCGGAAGATTTAGAAATTGACTTTTACCTTTAATAATTTCAT CATCAGATTTAATATTTCCACGATTAAACAATTTTAGATTTTGACTTCTTTTACCTTCATTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATACCCTCCTTTAATTAATCAAAATTTCATTACTTT AAATTTTATTATTTTCTCTCTTCATTTAAACGGCCTATCTTCAATTTTTAGATCAATCAATTTTATTTCTTCTATTA TAGTTATTAATAATAATAACTTTTTTCTTAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCAATTCCAGTACTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.35:Phenacoccus solenopsis 8
TTTTTTTTATGACTATACCTATTATTATCGGAAGATTTAGAAATTGACTTTTACCTTTAATAATTTCAT CATCAGATTTAATATTTCCACGATTAAACAATTTTAGATTTTGACTTCTTTTACCTTCATTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATACCCTCCTTTAATTAATCAAAATTTCATTACTTT AAATTTTATTATTTTCTCTCTTCATTTAAACGGCCTATCTTCAATTTTTAGATCAATCAATTTTATTTCTTCTATTA TAGTTATTAATAATAATAACTTTTTTCTTAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCAATTCCAGTACTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGATATGGAACTCCTATTCTTTATCAGCATTTATTT
SEQ ID NO.36:Phenacoccus solenopsis 9
TTTTTTTTATGACTATACCTATTATTATCGGAAGATTTAGAAATTGACTTTTACCTTTAATAATTTCAT CATCAGATTTAATATTTCCACGATTAAACAATTTTAGATTTTGACTTCTTTTACCTTCATTAATATTAATAATTTTA AATATAATATTAAATAATAATATTAATACAGGTTGAACTTTATACCCTCCTTTAATTAATCAAAATTTCATTACTTT AAATTTTATTATTTTCTCTCTTCATTTAAACGGCCTATCTTCAATTTTTAGATCAATCAATTTTATTTCTTCTATTA TAGTTATTAATAATAATAACTTTTTTCTTAATAATTTAACTTTATATACATGATCAATTATAATTACAACATTTTTA TTAATTGTTTCAATTCCAGTACTTTCAAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.37:Planococcus citri 1
TTTTCTTTATAACTATACCTATCATTATTGGAAGATTAAGAAATTGACTTTTACCATTAATATTAATAT CATCAGATTTAATTTTTCCCCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCACTTATTTTAATAATAATA AATATAATATTATCTAATAATATTAATACAGGTTGAACACTTTACCCTCCTTTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCTATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATCAATAATAATAATTTTTTTTTAAATAATATTACTTTATATATTTGATCTATTATTATTACAACTATTTTA TTAATTATTTCTATTCCAATTTTATCAAGAGCAATTACTATAATTATTTTAGATAATAATCTTAATATAAATTTTTT TAATCCATTAGGAAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.38:Planococcus citri 2
TTTTCTTTATAACTATACCTATCATTATTGGAAGATTAAGAAATTGACTTTTACCATTAATATTAATAT CATCAGATTTAATTTTTCCCCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCACTTATTTTAATAATAATA AATATAATATTATCTAATAATATTAATACAGGTTGAACACTTTACCCTCCTTTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCTATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATCAATAATAATAATTTTTTTTTAAATAATATTACTTTATATATTTGATCTATTATTATTACAACTATTTTA TTAATTATTTCTATTCCAATTTTATCAAGAGCAATTACTATAATTATTTTAGATAATAATCTTAATATAAATTTTTT TAATCCATTAGGAAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.39:Planococcus citri 3
TTTTCTTTATAACTATACCTATCATTATTGGAAGATTAAGAAATTGACTTTTGCCATTAATATTAATAT CATCAGATTTAATTTTTCCCCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCACTTATTTTAATAATAATA AATATAATATTATCTAATAATATTAATACAGGTTGAACACTTTACCCTCCTTTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCTATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATCAATAATAATAATTTTTTTTTTAATAATATTACTTTATATATTTGATCTATTATTATTACAACTATTTTA TTAATTATTTCTATTCCAATTTTATCAAGAGCAATTACTATAATTATTTTAGATAATAATCTTAATATAAATTTTTT TAATCCATTAGGAAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.40:Planococcus citri 4
TTTTCTTTATAACTATACCTATCATTATTGGAAGATTAAGAAATTGACTTTTGCCATTAATATTAATAT CATCAGATTTAATTTTTCCCCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCACTTATTTTAATAATAATA AATATAATATTATCTAATAATATTAATACAGGTTGAACACTTTACCCTCCTTTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCTATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATCAATAATAATAATTTTTTTTTTAATAATATTACTTTATATATTTGATCTATTATTATTACAACTATTTTA TTAATTATTTCTATTCCAATTTTATCAAGAGCAATTACTATAATTATTTTAGATAATAATCTTAATATAAATTTTTT TAATCCATTAGGAAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.41:Planococcus ficus 1
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTAAGAAATTGATTATTACCATTAATATTAATAT CTTCAGATTTAATTTTTCCACGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTTTTATTTTAATAATATTA AATATAATTTTATCTAATAATATTAATACCGGTTGAACTTTATATCCTCCTTTGATTAACCAAAATTTTATTACATT AAATTTTATCATTTTTTCTTTACATTTAAATGGAATTTCATCAATTTTTAGATCAATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATATTACTCTTTATATTTGATCAATTATTATTACAACCATTTTA TTAATTATTTCTATTCCTATTTTATCTAGAGCTATTACTATAATTATTCTAGATAACAATCTTAATATAAATTTTTT TAATCCTTTAGGAAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.42:Planococcus ficus 2
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTAAGAAATTGATTATTACCATTAATATTAATAT CTTCAGATTTAATTTTTCCACGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTTTTATTTTAATAATATTA AATATAATTTTATCTAATAATATTAATACCGGTTGAACTTTATATCCTCCTTTGATTAACCAAAATTTTATTACATT AAATTTTATCATTTTTTCTTTACATTTAAATGGAATTTCATCAATTTTTAGATCAATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATATTACTCTTTATATTTGATCAATTATTATTACAACCATTTTA TTAATTATTTCTATTCCTATTTTATCTAGAGCTATTACTATAATTATTCTAGATAACAATCTTAATATAAATTTTTT TAATCCTTTAGGAAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.43:Planococcus ficus 3
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTAAGAAATTGACTATTACCATTAATATTAATAT CTTCAGATTTAATTTTTCCACGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTCTTATTTTAATAATATTA AATATAATTTTATCTAATAATATTAATACTGGTTGAACTTTATATCCCCCTTTAATTAATCAAAATTTTATTACATT AAATTTTATCATTTTTTCTTTACATTTAAATGGTATTTCATCAATTTTTAGATCAATCAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATATTACTCTTTATATTTGATCTATTATTATCACAACTATTTTA TTAATTATTTCTATTCCTATTTTATCTAGAGCTATTACTATAATTATTTTAGATAATAATCTTAATATAAATTTTTT TAATCCCTTAGGAAATGGTAACCCAATTTTATACCAACATTTATTT
SEQ ID NO.44:Planococcus ficus 4
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTAAGAAATTGACTATTACCATTAATATTAATAT CTTCAGATTTAATTTTTCCACGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTCTTATTTTAATAATATTA AATATAATTTTATCTAATAATATTAATACTGGTTGAACTTTATATCCCCCTTTAATTAATCAAAATTTTATTACATT AAATTTTATCATTTTTTCTTTACATTTAAATGGTATTTCATCAATTTTTAGATCAATCAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATATTACTCTTTATATTTGATCTATTATTATCACAACTATTTTA TTAATTATTTCTATTCCTATTTTATCTAGAGCTATTACTATAATTATTTTAGATAATAATCTTAATATAAATTTTTT TAATCCCTTAGGAAATGGTAACCCAATTTTATACCAACATTTATTT
SEQ ID NO.45:Planococcus kraunhiae 1
TTTTTTTTATAACGATACCAATTATTATTGGAAGATTAAGAAATTGATTATTACCTTTAATACTTATAT CTTCAGATTTAATTTTTCCGCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTTTAATTCTTATGATATTA AATATAATTTTATCAAATAATATTAATACTGGTTGAACTTTATACCCCCCTTTGATTAACCAAAACTTCATTACATT AAATTTTATTATTTTTTCTCTACATTTAAATGGGATTTCTTCAATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATTAATAATAACAATTTTTTTCTTAATAATATTTCTTTATATATTTGATCAATTATTATTACTACAATTTTA TTAATTATTTCTATCCCTATCTTATCAAGAGCTATTACTATAATTATTTTAGATAATAATCTAAATATAAATTTTTT TAATCCATTAGGCAATGGAAACCCTATTCTTTATCAACATTTATTT
SEQ ID NO.46:Planococcus kraunhiae 2
TTTTTTTTATAACGATACCAATTATTATTGGAAGATTAAGAAATTGATTATTACCTTTAATACTTATAT CTTCAGATTTAATTTTTCCGCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTTTAATTCTTATGATATTA AATATAATTTTATCAAATAATATTAATACTGGTTGAACTTTATACCCCCCTTTGATTAACCAAAACTTCATTACATT AAATTTTATTATTTTTTCTCTACATTTAAATGGGATTTCTTCAATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATTAATAATAACAATTTTTTTCTTAATAATATTTCTTTATATATTTGATCAATTATTATTACTACAATTTTA TTAATTATTTCTATCCCTATCTTATCAAGAGCTATTACTATAATTATTTTAGATAATAATCTAAATATAAATTTTTT TAATCCATCAGGCAATGGAAACCCTATTCTTTATCAACATTTATTT
SEQ ID NO.47:Planococcus kraunhiae 3
TTTTTTTTATAACGATACCAATTATTATTGGAAGATTAAGAAATTGATTATTACCTTTAATACTTATAT CTTCAGATTTAATTTTTCCGCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTTTAATTCTTATGATATTA AATATAATTTTATCAAATAATATTAATACTGGTTGAACTTTATACCCCCCTTTGATTAACCAAAACTTCATTACATT AAATTTTATTATTTTTTCTCTACATTTAAATGGGATTTCTTCAATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATTAATAATAACAATTTTTTTCTTAATAATATTTCTTTATATATTTGATCAATTATTATTACTACAATTTTA TTAATTATTTCTATCCCTATCTTATCAAGAGCTATTACTATAATTATTTTAGATAATAATCTAAATATAAATTTTTT TAATCCATCAGGCAATGGAAACCCTATTCTTTATCAACATTTATTT
SEQ ID NO.48:Planococcus kraunhiae 4
TTTTTTTTATAACGATACCAATTATTATTGGAAGATTAAGAAATTGATTATTACCTTTAATACTTATAT CTTCAGATTTAATTTTTCCGCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTTTAATTCTTATGATATTA AATATAATTTTATCAAATAATATTAATACTGGTTGAACTTTATACCCCCCTTTGATTAACCAAAACTTCATTACATT AAATTTTATTATTTTTTCTCTACATTTAAATGGGATTTCTTCAATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATTAATAATAACAATTTTTTTCTTAATAATATTTCTTTATATATTTGATCAATTATTATTACTACAATTTTA TTAATTATTTCTATCCCTATCTTATCAAGAGCTATTACTATAATTATTTTAGATAATAATCTAAATATAAATTTTTT TAATCCATTAGGCAATGGAAACCCTATTCTTTATCAACATTTATTT
SEQ ID NO.49:Planococcus kraunhiae 5
TTTTTTTTATAACGATACCAATTATTATTGGAAGATTAAGAAATTGATTATTACCTTTAATACTTATAT CTTCAGATTTAATTTTTCCGCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTTTAATTCTTATGATATTA AATATAATTTTATCAAATAATATTAATACTGGTTGAACTTTATACCCCCCTTTGATTAACCAAAACTTCATTACATT AAATTTTATTATTTTTTCTCTACATTTAAATGGGATTTCTTCAATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATTAATAATAACAATTTTTTTCTTAATAATATTTCTTTATATATTTGATCAATTATTATTACTACAATTTTA TTAATTATTTCTATCCCTATCTTATCAAGAGCTATTACTATAATTATTTTAGATAATAATCTAAATATAAATTTTTT TAATCCATTAGGCAATGGAAACCCTATTCTTTATCAACATTTATTT
SEQ ID NO.50:Planococcus lilacinus 1
TTTTTTTTATAACTATACCAATTATTATTGGAAGAATAAGAAATTGATTATTACCATTAATATTAATAT CTTCAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCTTCATTAATTTTAATAATATTA AATATAATTTTAATAAATAATATTAATACGGGTTGAACTTTATACCCTCCATTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCATTACATTTAAATGGAATTTCTTCAATTTTTAGATCAATCAATTTTATTTCATCAATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATATTTCTTTATATATTTGATCAATTATTATTACTACAATTTTA TTAATTATTTCTATTCCTATTTTATCAAGAGCAATTACTATAATCATTCTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGTAATCCTATTCTCTATCAACATTTATTT
SEQ ID NO.51:Planococcus lilacinus 2
TTTTTTTTATAACTATACCAATTATTATTGGAAGAATAAGAAATTGATTATTACCATTAATATTAATAT CTTCAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCTTCATTAATTTTAATAATATTA AATATAATTTTAATAAATAATATTAATACGGGTTGAACTTTATACCCTCCATTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCATTACATTTAAATGGAATTTCTTCAATTTTTAGATCAATCAATTTTATTTCATCAATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATATTTCTTTATATATTTGATCAATTATTATTACTACAATTTTA TTAATTATTTCTATTCCTATTTTATCAAGAGCAATTACTATAATCATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGTAATCCTATTCTCTATCAACATTTATTT
SEQ ID NO.52:Planococcus lilacinus 3
TTTTTTTTATAACTATACCAATTATTATTGGAAGAATAAGAAATTGATTATTACCATTAATATTAATAT CTTCAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCTTCATTAATTTTAATAATATTA AATATAATTTTAATAAATAATATTAATACGGGTTGAACTTTATACCCTCCATTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCATTACATTTAAATGGAATTTCTTCAATTTTTAGATCAATCAATTTTATTTCATCAATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATATTTCTTTATATATTTGATCAATTATTATTACTACAATTTTA TTAATTATTTCTATTCCTATTTTATCAAGAGCAATTACTATAATCATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGTAATCCTATTCTCTATCAACATTTATTT
SEQ ID NO.53:Planococcus lilacinus 4
TTTTTTTTATAACTATACCAATTATTATTGGAAGAATAAGAAATTGATTATTACCATTAATATTAATAT CTTCAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCTTCATTAATTTTAATAATATTA AATATAATTTTAATAAATAATATTAATACAGGTTGAACTTTATACCCCCCATTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCATTACATTTAAATGGAATTTCTTCAATTTTTAGATCAATCAATTTTATTTCATCAATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATATTTCTTTATATATTTGATCAATTATTATCACTACAATTTTA TTAATTATTTCTATTCCTATTTTATCAAGAGCAATTACTATAATTATTCTAGATAATAATTTAAATATAAATTTTTT TAATCCTTTAGGAAATGGTAATCCTATTCTCTATCAACATTTATTT
SEQ ID NO.54:Planococcus miNO.r 1
TTTTTTTTATAACTATACCTATCATTATTGGAAGATTAAGAAATTGACTCTTACCATTAATATTAATAT CATCAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCACTTATTTTAATAATAATA AATATAATATTATCTAATAATATTAATACCGGTTGAACACTTTATCCCCCTTTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGGATTTCTTCTATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATTAATAATAATAACTTTTTTTTAAATAATATTACTTTATATATTTGATCCATTATTATTACAACTATTTTA TTAATTATTTCTATTCCAATTTTATCAAGAGCAATTACTATAATTATTTTAGATAATAATCTTAATATAAATTTTTT TAATCCATTAGGAAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.55:Planococcus miNO.r 2
TTTTTTTTATAACTATACCTATCATTATTGGAAGATTAAGAAATTGACTCTTACCATTAATATTAATAT CATCAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCACTTATTTTAATAATAATA AATATAATATTATCTAATAATATTAATACCGGTTGAACACTTTATCCCCCTTTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGGATTTCTTCTATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATTAATAATAATAACTTTTTTTTAAATAATATTACTTTATATATTTGATCCATTATTATTACAACTATTTTA TTAATTATTTCTATTCCAATTTTATCAAGAGCAATTACTATAATTATTTTAGATAATAATCTTAATATAAATTTTTT TAATCCATTAGGAAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.56:Planococcus miNO.r 3
TTTTTTTTATAACTATACCTATCATTATTGGAAGATTAAGAAATTGACTCTTACCATTAATATTAATAT CATCAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCACTTATTTTAATAATAATA AATATAATATTATCTAATAATATTAATACCGGTTGAACACTTTATCCCCCTTTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGGATTTCTTCTATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATTAATAATAATAACTTTTTTTTAAATAATATTACTTTATATATTTGATCCATTATTATTACAACTATTTTA TTAATTATTTCTATTCCAATTTTATCAAGAGCAATTACTATAATTATTTTAGATAATAATCTTAATATAAATTTTTT TAATCCATTAGGAAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.57:Planococcus miNO.r 4
TTTTTTTTATAACTATACCTATCATTATTGGAAGATTAAGAAATTGACTCTTACCATTAATATTAATAT CATCAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCACTTATTTTAATAATAATA AATATAATATTATCTAATAATATTAATACCGGTTGAACACTTTATCCCCCTTTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGGATTTCTTCTATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATTAATAATAATAACTTTTTTTTAAATAATATTACTTTATATATTTGATCCATTATTATTACAACTATTTTA TTAATTATTTCTATTCCAATTTTATCAAGAGCAATTACTATAATTATTTTAGATAATAATCTTAATATAAATTTTTT TAATCCATTAGGAAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.58:Planococcus miNO.r 5
TTTTTTTTATAACTATACCTATCATTATTGGAAGATTAAGAAATTGACTCTTACCATTAATATTAATAT CATCAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCACTTATTTTAATAATAATA AATATAATATTATCTAATAATATTAATACCGGTTGAACACTTTATCCCCCTTTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGGATTTCTTCTATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATTAATAATAATAACTTTTTTTTAAATAATATTACTTTATATATTTGATCCATTATTATTACAACTATTTTA TTAATTATTTCTATTCCAATTTTATCAAGAGCAATTACTATAATTATTTTAGATAATAATCTTAATATAAATTTTTT TAATCCATTAGGAAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.59:Planococcus vovae
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTAAGAAATTGACTTTTACCATTAATATTAATAT CATCAGATTTAATTTTTCCACGATTAAATAATTTTAGATTTTGATTACTTTTTCCTTCATTAATTCTTATAATATTA AATATAATTTTATCTAATAATATTAATACAGGATGAACTTTATATCCTCCTTTAATTAATCAAAATTTTATTACATT AAATTTCATTATTTTTTCTTTACATTTAAACGGAATTTCATCAATCTTTAGATCAATTAATTTTATTTCTTCGATTT TTATTATTAATAACAACAATTTTTTTTTAAATAATATTTCCCTCTATATTTGATCAATCATTATCACTACAATTTTA CTAATTATTTCTATTCCAATTTTATCTAGAGCTATTACCATAATTATTTTAGATAATAATTTAAATATAAATTTCTT TAATCCTTTAGGAAATGGTAATCCAATTCTTTATCAACATTTATTT
SEQ ID NO.60:Pseudococcus baliteus 1
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAAGAAATTGATTATTACCTTTAATACTTTCAT CTTCTGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTATATTATTTTTAATATTA AATATACTATTAAATAATAATATTAATACAGGATGAACTTTATATCCTCCTTTAATTAATCAAAATTTTATTACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGTTCAATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTATTAATAATTTATCTTTATATATTTGATCAATTATTATTACAACTATTTTA TTAATTATTTCTATTCCTATTTTATCTAGAGCTATTACTATAATTATTTTAGACAATAATTTAAATATAAATTTTTT TAATCCTATAGGTAATGGTAATCCTATTTTATATCAACATTTATTT
SEQ ID NO.61:Pseudococcus baliteus 2
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAAGAAATTGATTATTACCTTTAATACTTTCAT CTTCTGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTATATTATTTTTAATATTA AATATACTATTAAATAATAATATTAATACAGGATGAACTTTATATCCTCCTTTAATTAATCAAAATTTTATTACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGTTCAATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTATTAATAATTTATCTTTATATATTTGATCAATTATTATTACAACTATTTTA TTAATTATTTCTATTCCTATTTTATCTAGAGCTATTACTATAATTATTTTAGACAATAATTTAAATATAAATTTTTT TAATCCTATAGGTAATGGTAATCCTATTTTATACCAACATTTATTT
SEQ ID NO.62:Pseudococcus baliteus 3
TTTTTTTTATAACTATACCTATTATTATTGGTAGGTTAAGAAATTGATTATTACCTTTAATACTTTCAT CTTCTGATTTAATTTTTCCTCGATTAAATAATTTTAGATTCTGATTATTAATTCCATCTATATTATTTTTAATATTA AATATATTATTAAATAATAATATTAATACAGGATGAACTTTATACCCTCCTTTAATTAATCAAAATTTTATTACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGTTCAATTAATTTTATTTCCTCAATTT TTATTATTAATAATAATAATTTTTTTATTAATAATTTATCTTTATATATTTGATCAATTATTATTACAACTATTTTA TTAATTATTTCTATTCCTATTTTATCTAGAGCTATTACTATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTATAGGTAATGGTAATCCTATTTTATATCAACATTTATTC
SEQ ID NO.63:Pseudococcus baliteus 4
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAAGAAATTGATTATTACCTTTAATACTTTCAT CTTCTGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTATATTATTTTTAATATTA AATATACTATTAAATAATAATATTAATACAGGATGAACTTTATATCCTCCTTTAATTAATCAAAATTTTATTACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGTTCAATTAATTTTATTTCTTCAATTT TTATTGTTAATAATAATAATTTTTTTATTAATAATTTATCTTTATATATTTGATCAATTATTATTACAACTATTTTA TTAATTATTTCTATTCCTATTTTATCTAGAGCTATTACTATAATTATTTTAGACAATAATTTAAATATAAATTTTTT TAATCCTATAGGTAATGGTAATCCTATTTTATATCAACATTTATTT
SEQ ID NO.64:Pseudococcus baliteus 5
TTTTTTTTATAACCATACCTATTATTATTGGTAGATTAAGAAATTGATTATTACCTTTAATACTTTCAT CTTCTGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTATATTATTTTTAATATTA AATATACTATTAAATAATAATATTAATACAGGATGAACTTTATATCCTCCTTTAATTAATCAAAATTTTATTACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGTTCAATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTATTAATAATTTATCTTTATATATTTGATCAATTATTATTACAACTATTTTA TTAATTATTTCTATTCCTATTTTATCTAGAGCTATTACTATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTATAGGTAATGGTAATCCTATTTTATATCAACATTTATTT
SEQ ID NO.65:Pseudococcus baliteus 6
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAAGAAATTGATTATTACCTTTAATACTTTCAT CTTCTGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTATATTATTTTTAATATTA AATATATTATTAAATAATAATATTAATACAGGATGAACTTTATATCCTCCTTTAATTAATCAAAATTTTATTACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGTTCAATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTATTAATAATTTATCTTTATATATTTGATCAATTATTATTACAACTATTTTA TTAATTATTTCTATTCCTATTTTATCTAGAGCTATTACTATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTATAGGTAATGGTAATCCTATTTTATATCAACATTTATTT
SEQ ID NO.66:Pseudococcus baliteus 7
TTTTTTTTATAACCATACCTATTATTATTGGTAGATTAAGAAATTGATTATTACCTTTAATACTTTCAT CTTCTGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCATCTATATTATTTTTAATATTA AATATACTATTAAATAATAATATTAATACAGGATGAACTTTATATCCTCCTTTAATTAATCAAAATTTTATTACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGTTCAATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTATTAATAATTTATCTTTATATATTTGATCAATTATTATTACAACTATTTTA TTAATTATTTCTATTCCTATTTTATCTAGAGCTATTACTATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTATAGGTAATGGTAATCCTATTTTATATCAACATTTATTT
SEQ ID NO.67:Pseudococcus calceolariae 1
TTTTTTTCATAACTATACCTATTATTATTGGAAGATTAAGAAATTGATTATTACCCTTAATATTAATAT CTTCAGATTTAATTTTTCCCCGATTAAACAATTTTAGATTTTGATTACTTATTCCTTCAATAATTTTTATAATATTA AATATAATATTAAATAATAATATTAATTCTGGATGAACATTGTATCCCCCTTTAATTAACCAAAATTTCATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGTATTTCCTCAATTTTTAGATCAATTAATTTTATTTCTTCTATTT TTATTATTAATAATAACAATTTTTTTGTTAATAATATATCTTTATTTATTTGATCTATTATTATTACCACTATTTTA TTAATTATTTCTATTCCCATTCTTTCTAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT CAATCCAATAGGCAATGGAAATCCAATTCTTTACCAACATTTATTT
SEQ ID NO.68:Pseudococcus calceolariae 2
TTTTTTTCATAACTATACCTATTATTATTGGAAGATTAAGAAATTGATTATTACCCTTAATATTAATAT CTTCAGATTTAATTTTTCCCCGATTAAACAATTTTAGATTTTGATTACTTATTCCTTCAATAATTTTTATAATATTA AATATAATATTAAATAATAATATTAATTCTGGATGAACATTGTATCCCCCTTTAATTAACCAAAATTTCATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGTATTTCCTCAATTTTTAGATCAATTAATTTTATTTCTTCTATTT TTATTATTAATAATAACAATTTTTTTGTTAATAATATATCTTTATTTATTTGATCTATTATTATTACCACTATTTTA TTAATTATTTCTATTCCCATTCTTTCTAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT CAATCCAATAGGCAATGGAAATCCAATTCTTTACCAACATTTATTT
SEQ ID NO.69:Pseudococcus calceolariae 3
TTTTTTTCATAACTATACCTATTATTATTGGAAGATTAAGAAATTGATTATTACCCTTAATATTAATAT CTTCAGATTTAATTTTTCCCCGATTAAACAATTTTAGATTTTGATTACTTATTCCTTCAATAATTTTTATAATATTA AATATAATATTAAATAATAATATTAATTCTGGATGAACATTGTATCCCCCTTTAATTAACCAAAATTTCATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGTATTTCCTCAATTTTTAGATCAATTAATTTTATTTCTTCTATTT TTATTATTAATAATAACAATTTTTTTGTTAATAATATATCTTTATTTATTTGATCTATTATTATTACCACTATTTTA TTAATTATTTCTATTCCCATTCTTTCTAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT CAATCCAATAGGCAATGGAAATCCAATTCTTTACCAACATTTATTT
SEQ ID NO.70:Pseudococcus calceolariae 4
TTTTTTTCATAACTATACCTATTATTATTGGAAGATTAAGAAATTGATTATTACCCTTAATATTAATAT CTTCAGATTTAATTTTTCCCCGATTAAACAATTTTAGATTTTGATTACTTATTCCTTCAATAATTTTTATAATATTA AATATAATATTAAATAATAATATTAATTCTGGATGAACATTGTATCCCCCTTTAATTAACCAAAATTTCATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGTATTTCCTCAATTTTTAGATCAATTAATTTTATTTCTTCTATTT TTATTATTAATAATAACAATTTTTTTGTTAATAATATATCTTTATTTATTTGATCTATTATTATTACCACTATTTTA TTAATTATTTCTATTCCCATTCTTTCTAGAGCTATTACAATAATTATTTTAGATAATAATTTAAATATAAATTTTTT CAATCCAATAGGCAATGGAAATCCAATTCTTTACCAACATTTATTT
SEQ ID NO.71:Pseudococcus comstocki 1
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTAAGAAATTGATTATTACCTTTAATATTAATAT CATCTGATTTAATTTTTCCTCGTTTAAATAATTTTAGATTTTGATTATTAATTCCTTCATTAATTTTAATAATATTA AATATATTTTTAAATAATAATATTAATACAGGTTGAACATTATATCCTCCATTAATTAATCAAAATTATATTTCATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATTAATAATAATAATTTTTTATTAAATAACTCATCTTTATATATTTGATCTATTATTATTACTACAATTCTT TTAATTATTTCTATTCCTATTTTATCTAGAGCTATTACTATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTATAGGAAATGGAAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.72:Pseudococcus comstocki 2
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTAAGAAATTGATTATTACCTTTGATATTAATAT CATCTGATTTAATTTTTCCTCGTTTAAATAATTTTAGATTTTGATTATTAATTCCTTCATTAATTTTAATAATATTA AATATATTTTTAAATAATAATATTAATACAGGTTGAACATTATACCCTCCTTTAATTAATCAAAATTATATTTCATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATTAATAATAACAATTTTTTATTAAATAATTTATCTTTATATATTTGATCAATTATTATTACTACAATTCTT TTAATTATTTCCATTCCTGTTTTATCTAGAGCTATTACTATAATTATTTTAGATAATAATTTAAATATAAATTTTTT CAATCCTATAGGAAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.73:Pseudococcus comstocki 3
TTTTTTTTATAACTATACCTATTATTATTGGAAGTTTAAGAAATTGATTATTACCTTTAATATTAATAT CTTCGGATTTAATTTTTCCCCGATTAAATAATTTTAGATTTTGATTATTAATTCCTTCATTAATTCTTTTAATATTA AATATAATATTAAATAATAATATTAATACAGGTTGAACATTATATCCGCCATTAATTAATCAAAATTATATCACATT AAATTTTATTATTTTTTCTTTACATTTAAACGGTATTTCTTCAATTTTTAGTTCTATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTGTTAATAATTTATCTTTATATATTTGATCAATTATTATTACTACAATTCTT TTAATTATTTCTATTCCTATTTTATCAAGAGCAATTACAATAATTATTTTAGATAATAATATACATATAAGTTTTTT TAACCCTATAGGTAATGGCAATCCTATTTTATATCAACATTTATTT
SEQ ID NO.74:Pseudococcus comstocki 4
TTTTTTTTATAACTATACCTATTATTATTGGAAGTTTAAGAAATTGATTATTACCTTTAATATTAATAT CTTCGGATTTAATTTTTCCCCGATTAAATAATTTTAGATTTTGATTATTAATTCCTTCATTAATTCTTTTAATATTA AATATAATATTAAATAATAATATTAATACAGGTTGAACATTATATCCGCCATTAATTAATCAAAATTATATCACATT AAATTTTATTATTTTTTCTTTACATTTAAACGGTATTTCTTCAATTTTTAGTTCTATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTGTTAATAATTTATCTTTATATATTTGATCAATTATTATTACTACAATTCTT TTAATTATTTCTATTCCTATTTTATCAAGAGCAATTACAATAATTATTTTAGATAATAATATACATATAAGTTTTTT TAACCCTATAGGTAATGGCAATCCTATTTTATATCAACATTTATTT
SEQ ID NO.75:Pseudococcus comstocki 5
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTAAGAAATTGATTATTACCTTTAATATTAATAT CATCTGATTTAATTTTTCCTCGTTTAAATAATTTTAGATTTTGATTATTAATTCCTTCATTAATTTTAATAATATTA AATATATTTTTAAATAATAATATTAATACAGGTTGAACATTATATCCTCCATTAATTAATCAAAATTATATTTCATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGATCAATTAATTTTATTTCATCAATTT TTATTATTAATAATAATAATTTTTTATTAAATAACTTATCTTTATATATTTGATCTATTATTATTACTACAATTCTT TTAATTATTTCTATTCCTATTTTATCTAGAGCTATTACTATAATTATTTTAGATAATAATTTAAATATAAATTTTTT TAATCCTATAGGAAATGGAAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.76:Pseudococcus cryptus 1
TTTTTTTTATAACTATACCTATTATTATTGGTAGTTTAAGAAATTGATTATTACCATTAATATTAATAT CTTCTGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCTTCATTAATTCTTTTAATATTA AATATAATATTAAATAATAATATTAATACAGGTTGAACATTATACCCTCCATTAATTAATCAAAATTATATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGATCAATCAATTTTATTTCTTCAATTT TTATTATTAATAATAACAATTTTTTCATTAATAATTTATCTTTATATATTTGATCAATTATTATTACTACAATTCTT TTAATTATTTCTATTCCTATTTTATCAAGAGCAATTACAATAATTATTTTAGATAATAATATACATATAAATTTTTT TAATCCTTTAGGTAATGGCAATCCTATTTTATATCAACATTTATTT
SEQ ID NO.77:Pseudococcus cryptus 2
TTTTTTTTATAACTATACCTATTATTATTGGTAGTTTAAGAAATTGATTATTACCATTAATATTAATAT CTTCTGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCTTCATTAATTCTTTTAATATTA AATATAATATTAAATAATAATATTAATACAGGTTGAACATTATACCCTCCATTAATTAATCAAAATTATATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGATCAATCAATTTTATTTCTTCAATTT TTATTATTAATAATAACAATTTTTTCATTAATAATTTATCTTTATATATTTGATCAATTATTATTACTACAATTCTT TTAATTATTTCTATTCCTATTTTATCAAGAGCAATTACAATAATTATTTTAGATAATAATATACATATAAATTTTTT TAATCCTTTAGGTAATGGCAATCCTATTTTATATCAACATTTATTT
SEQ ID NO.78:Pseudococcus cryptus 3
TTTTTTTTATAACTATACCTATTATTATTGGTAGTTTAAGAAATTGATTATTACCATTAATATTAATAT CTTCTGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCTTCATTAATTCTTTTAATATTA AATATAATATTAAATAATAATATTAATACAGGTTGAACATTATACCCTCCATTAATTAATCAAAATTATATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGATCAATCAATTTTATTTCTTCAATTT TTATTATTAATAATAACAATTTTTTCATTAATAATTTATCTTTATATATTTGATCAATTATTATTACTACAATTCTT TTAATTATTTCTATTCCTATTTTATCAAGAGCAATTACAATAATTATTTTAGATAATAATATACATATAAATTTTTT TAATCCTTTAGGTAATGGCAATCCTATTTTATATCAACATTTATTT
SEQ ID NO.79:Pseudococcus cryptus 4
TTTTTTTTATAACTATACCTATTATTATTGGTAGTTTAAGAAATTGATTATTACCATTAATATTAATAT CTTCTGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGATTATTAATTCCTTCATTAATTCTTTTAATATTA AATATAATATTAAATAATAATATTAATACAGGTTGAACATTATACCCTCCATTAATTAATCAAAATTATATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATTTCTTCAATTTTTAGATCAATCAATTTTATTTCTTCAATTT TTATTATTAATAATAACAATTTTTTCATTAATAATTTATCTTTATATATTTGATCAATTATTATTACTACAATTCTT TTAATTATTTCTATTCCTATTTTATCAAGAGCAATTACAATAATTATTTTAGATAATAATATACATATAAATTTTTT TAATCCTTTAGGTAATGGCAATCCTATTTTATATCAACATTTATTT
SEQ ID NO.80:Pseudococcus jackbeardsleyi 1
TTTTTTTTATAACTATACCTATTATTATTGGAAGAATAAGAAATTGATTATTACCATTAATAATAATAT CTAGAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATTCCTTCATTAATATTAATAATATTA AATATAATATTAAATAATAATATTAATACAGGATGAACATTATATCCTCCTTTAATTAATCAAAATTATATTTCATT AAATTTTATTATTTTTTCATTACATTTAAATGGAATTTCATCAATTTTCAGATCAATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATTTAACACTTTATATTTGATCTATTATTATTACATCTATTTTA TTAATTCTTTCTATTCCAATTTTATCAAGAGCAATTACAATAATTATCTTAGATAGAAATTTTAATATAAATTTTTT TAATCCTATAGGAAATGGAAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.81:Pseudococcus jackbeardsleyi 2
TTTTTTTTATAACTATACCTATTATTATTGGAAGAATAAGAAATTGATTATTACCATTAATAATAATAT CTAGAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATTCCTTCATTAATATTAATAATATTA AATATAATATTAAATAATAATATTAATACAGGATGAACATTATATCCTCCTTTAATTAATCAAAATTATATTTCATT AAATTTTATTATTTTTTCATTACATTTAAATGGAATTTCATCAATTTTCAGATCAATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATTTAACACTTTATATTTGATCTATTATTGTTACATCTATTTTA TTAATTCTTTCTATCCCAATTTTATCAAGAGCAATTACAATAATTATCTTAGATAGAAATTTTAATATAAATTTTTT TAATCCTATAGGAAATGGAAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.82:Pseudococcus jackbeardsleyi 3
TTTTTTTTATAACTATACCTATTATTATTGGAAGAATAAGAAATTGATTATTACCATTAATAATAATAT CTAGAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATTCCTTCATTAATATTAATAATATTA AATATAATATTAAATAATAATATTAATACAGGATGAACATTATATCCTCCTTTAATTAATCAAAATTATATTTCATT AAATTTTATTATTTTTTCATTACATTTAAATGGAATTTCATCAATTTTCAGATCAATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATTTAACACTTTATATTTGATCTATTATTGTTACATCTATTTTA TTAATTCTTTCTATCCCAATTTTATCAAGAGCAATTACAATAATTATCTTAGATAGAAATTTTAATATAAATTTTTT TAATCCTATAGGAAATGGAAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.83:Pseudococcus jackbeardsleyi 4
TTTTTTTTATAACTATACCTATTATTATTGGAAGAATAAGAAATTGATTATTACCATTAATAATAATAT CTAGAGATTTAATTTTTCCTCGATTAAATAATTTTAGATTTTGACTTTTAATTCCTTCATTAATATTAATAATATTA AATATAATATTAAATAATAATATTAATACAGGATGAACATTATATCCTCCTTTAATTAATCAAAATTATATTTCATT AAATTTTATTATTTTTTCATTACATTTAAATGGAATTTCATCAATTTTCAGATCAATTAATTTTATTTCTTCAATTT TTATTATTAATAATAATAATTTTTTTTTAAATAATTTAACACTTTATATTTGATCTATTATTATTACATCTATTTTA TTAATTCTTTCTATTCCAATTTTATCAAGAGCAATTACAATAATTATCTTAGATAGAAATTTTAATATAAATTTTTT TAATCCTATAGGAAATGGAAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.84:Pseudococcus longispinus 1
TTTTTTTCATAACCATACCTATTATTATCGGTAGATTAAGAAATTGACTTCTACCATTAATATTAATAT CATCAGACTTAATTTTCCCGCGTTTAAATAATTTTAGATTTTGATTACTTATACCATCATTAATTTTTATAATATTA AATATATTATTAAATAATAATATTAATACTGGATGAACTCTTTATCCTCCTTTAATTAATCAAAATTTTATTACACT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATCTCCTCAATTTTTAGATCTATTAATTTTATTTCATCAATTT TTATTATTAATAATAATAATTTTTTATTAAATAATTTATCACTATATATTTGATCAATTATTATTACTACTATTTTA TTAATTATTTCTATTCCTATTTTATCAAGGGCAATTACCATAATTATTTTAGATAATAACTTAAATATAAATTTCTT TAATCCTATAGGTAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.85:Pseudococcus longispinus 2
TTTTTTTCATAACCATACCTATTATTATCGGTAGATTAAGAAATTGACTTCTACCATTAATATTAATAT CATCAGATTTAATTTTCCCACGTTTAAATAATTTTAGATTTTGATTACTTATACCATCATTAATTTTTATAATATTA AATATATTATTAAATAATAATATTAATACTGGATGAACTCTTTATCCTCCTTTAATTAATCAAAATTTTATTACATT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATCTCTTCAATTTTTAGATCTATTAATTTTATTTCATCAATTT TTATTATTAATAATAATAATTTTTTATTAAATAATTTATCATTATATATCTGATCAATTATTATTACTACTATTTTA TTAATTATTTCTATTCCTATTTTATCAAGAGCAATTACCATAATTATTTTAGATAATAACTTAAATATAAATTTTTT TAATCCTATAGGTAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.86:Pseudococcus longispinus 3
TTTTTTTCATAACCATACCTATTATTATCGGTAGATTAAGAAATTGACTTCTACCATTAATATTAATAT CATCAGACTTAATTTTCCCGCGTTTAAATAATTTTAGATTTTGATTACTTATACCATCATTAATTTTTATAATATTA AATATATTATTAAATAATAATATTAATACTGGATGAACTCTTTATCCTCCTTTAATTAATCAAAATTTTATTACACT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATCTCCTCAATTTTTAGATCTATTAATTTTATTTCATCAATTT TTATTATTAATAATAATAATTTTTTATTAAATAATTTATCACTATATATTTGATCAATTATTATTACTACTATTTTA TTAATTATTTCTATTCCTATTTTATCAAGGGCAATTACCATAATTATTTTAGATAATAACTTAAATATAAATTTCTT TAATCCTATAGGTAATGGTAATCCAATTTTATATCAACATTTATTT
SEQ ID NO.87:Pseudococcus longispinus 4
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAAGTAATTGACTTTTACCATTAATATTAATAT CATCAGATTTAATTTTTCCACGTTTAAATAATTTTAGATTCTGATTACTTATTCCATCATTAATTTTTATAATATTA AATATATTACTAAATAATAATATTAATACTGGATGAACTCTTTATCCACCTTTAATTAATCAAAATTATATTACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGAATCTCATCAATTTTTAGATCTATTAATTTTATTTCATCAATTT TTATTATTAATAATAATAATTTTTTATTAAATAATTTATCATTATATATTTGATCAATTATTATTACTACTATTTTA TTAATTATTTCTATTCCTATTTTATCAAGAGCAATTACTATAATTATTTTAGATAACAATTTAAACATAAATTTTTT TAATCCAATAGGTAATGGTAATCCAATTTTATACCAACATCTATTT
SEQ ID NO.88:Pseudococcus longispinus 5
TTTTTTTTATAACTATACCTATTATTATTGGTAGATTAAGTAATTGACTTTTACCATTAATATTAATAT CATCAGATTTAATTTTTCCACGTTTAAATAATTTTAGATTCTGATTACTTATTCCATCATTAATTTTTATAATATTA AATATATTACTAAATAATAATATTAATACTGGATGAACTCTTTATCCACCTTTAATTAATCAAAATTATATTACTTT AAATTTTATTATTTTTTCTTTACATTTAAATGGAGTCTCATCAATTTTTAGATCTATTAATTTTATTTCATCAATTT TTATTATTAATAATAATAATTTTTTATTAAATAATTTATCATTATATATTTGATCAATTATTATTACTACTATTTTA TTAATTATTTCTATTCCTATTTTATCAAGAGCAATTACTATAATTATTTTAGATAACAATTTAAACATAAATTTTTT TAATCCAATAGGTAATGGTAATCCAATTTTATACCAACATCTATTT
SEQ ID NO.89:Pseudococcus viburni 1
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTAAGAAATTGACTTTTACCTTTAATATTAATAT CTAGAGATTTAATTTTTCCTCGTTTAAATAATTTTAGATTTTGATTATTAATTCCTTCTTTACTATTAATAATATTT AATATAATATTAAATAATAATATTAATACAGGTTGAACACTTTATCCTCCTTTAATTAATCAAAATTTTTTAACATT AAATTTTATTATTTTTTCATTACATTTAAATGGAATTTCTTCTATTTTTAGATCAATTAATTTTATTTCTTCTATTT TTATTATTAATAATAATAATTTTTTATTTAATAACTTATCATTATATATTTGATCCATCATTATTACTTCTATTCTT TTAATTCTTTCTATTCCTATTTTATCAAGAGCTATTACAATAATTATTCTTGATAGAAATTTTAATATACATTTTTT TAATCCATTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.90:Pseudococcus viburni 2
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTAAGAAATTGACTTTTACCTTTAATATTAATAT CTAGAGATTTAATTTTTCCTCGTTTAAATAATTTTAGATTTTGATTATTAATTCCTTCTTTACTATTAATAATATTT AATATAATATTAAATAATAATATTAATACAGGTTGAACACTTTATCCTCCTTTAATTAATCAAAATTTTTTAACATT AAATTTTATTATTTTTTCATTACATTTAAATGGAATTTCTTCTATTTTTAGATCAATTAATTTTATTTCTTCTATTT TTATTATTAATAATAATAATTTTTTATTTAATAACTTATCATTATATATTTGATCCATCATTATTACTTCTATTCTT TTAATTCTTTCTATTCCTATTTTATCAAGAGCTATTACAATAATTATTCTTGATAGAAATTTTAATATACATTTTTT TAATCCATTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.91:Pseudococcus viburni 3
TTTTTTTTATAACTATACCTATTATTATTGGGAGATTAAGAAATTGACTTTTACCTTTAATATTAATAT CTAGAGATTTAATTTTTCCTCGTTTAAATAATTTTAGATTTTGATTATTAATTCCTTCTTTACTATTAATAATATTT AATATAATATTAAATAATAATATTAATACAGGTTGAACACTTTATCCTCCTTTAATTAATCAAAATTTTTTAACATT AAATTTTATTATTTTTTCATTACATTTAAATGGAATTTCTTCTATTTTTAGATCAATTAATTTTATTTCTTCTATTT TTATTATTAATAATAATAATTTTTTATTTAATAACTTATCATTATATATTTGATCCATCATTATTACTTCTATTCTT TTAATTCTTTCTATTCCTATTTTATCAAGAGCTATTACAATAATTATTCTTGATAGAAATTTTAATATACATTTTTT TAATCCATTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.92:Pseudococcus viburni 4
TTTTTTTTATAACTATACCTATTATCATTGGAAGATTAAGAAATTGACTTTTACCTTTAATATTAATAT CTAGAGATTTAATTTTTCCTCGTTTAAATAATTTTAGATTTTGATTATTAATTCCTTCTTTACTATTAATAATATTT AATATAATATTAAATAATAATATTAATACAGGTTGAACACTTTATCCTCCTTTAATTAATCAAAATTTTTTAACATT AAATTTTATTATTTTTTCATTACATTTAAATGGAATTTCTTCTATTTTTAGATCAATTAATTTTATTTCTTCTATTT TTATTATTAATAATAATAATTTTTTATTTAATAACTTATCATTATATATTTGATCCATCATTATTACTTCTATTCTT TTAATTCTTTCTATTCCTATTTTATCAAGAGCTATTACAATAATTATTCTTGATAGAAATTTTAATATACATTTTTT TAATCCATTAGGAAATGGAAATCCTATTCTTTATCAACATTTATTT
SEQ ID NO.93:Pseudococcus viburni 5
TTTTTTTTATAACTATACCTATTATTATTGGAAGATTAAGAAATTGACTTTTACCTTTAATATTAATAT CTAGAGATTTAATTTTTCCTCGTTTAAATAATTTTAGGTTTTGATTATTAATTCCTTCTTTATTATTAATAATATTT AATATAATATTAAATAATAATATTAATACAGGTTGAACACTTTATCCTCCTTTAATTAATCAAAATTTTTTAACGTT AAATTTTATTATCTTTTCATTACATTTAAATGGGATTTCTTCTATTTTTAGATCAATTAATTTTATTTCTTCTATTT TTATTATTAATAATAATAATTTTTTATTTAATAATTTGTCATTATATATTTGATCTATCATTATCACTTCTATTCTT TTAATTCTTTCTATTCCTATTTTATCAAGAGCTATTACAATAATTATTCTTGATAGAAATTTTAATATACATTTTTT TAATCCATTAGGAAATGGAAACCCTATTCTTTATCAACATTTATTT

Claims (6)

1. a kind of Pseudococcidae insect genes barcode detection kit, it is characterised in that: include at least a genetic barcode sequence More than disk and 20 dosages primer PseJ and primer PseN；The primer PseJ and primer PseN Serial No.,
PseJ:5'- CCTTCAACTAATCATAAAAATATNAG -3'；
PseN:5'-TAAACTTCTGGATGTCCAAAAAATCA-3；
There are 93 standard sequence of barcodes, the standard sequence of barcodes such as SEQ ID NO.1 in the genetic barcode sequence disk To shown in SEQ ID NO.93.
2. a kind of Pseudococcidae insect genes barcode detection kit according to claim 1, it is characterised in that: the examination Agent box further includes the following reagent of 20 dosages or more: Taq buffer, MgCl2, dNTP mix, Taq DNA polymerase, ddH2O, DNA standard sample.
3. a kind of detection method of Pseudococcidae insect genes barcode detection kit, which is characterized in that with the kit into Row amplification the following steps are included:
The preparation of S1, DNA profiling: reagent is extracted using GenMagBio zooblast tissue/cell genomic DNA paramagnetic particle method Box extracts sample to be tested；
The amplification of S2, DNA bar code sequence: using the DNA test sample in S1 as template, using primer PseJ and primer PseN PCR amplification, the primer PseJ and primer PseN Serial No. are carried out,
PseJ:5'- CCTTCAACTAATCATAAAAATATNAG -3'；
PseN:5'-TAAACTTCTGGATGTCCAAAAAATCA-3；The PCR reaction system uses standards system, and total volume is 50 μ L or 25 μ L；
The PCR reaction condition: 95 DEG C of initial denaturation 4min；35 circulations: 94 DEG C of deformation 1min, 48 DEG C of annealing 1min, 72 DEG C are prolonged Stretch 90s；Last 72 DEG C of extensions 4min, 4 DEG C of preservations；
S3,8 μ L samples to be tested of mixing and 2 μ L 6 × Glycerol DNA sample-loading buffer loadings, with DNA Marker DL100 For molecular weight marker, 120 V of constant pressure, is dyed, gel with the EB of 3% agarose gel electrophoresis 45min, 0.5% μm of ol/L at room temperature Imaging system test sample, photographic analysis；
S4, PCR result judgement:
8 μ L PCR products and 2 μ L 6 × Glycerol DNA sample-loading buffer loadings are mixed, are with DNA Marker DL100 Molecular weight marker, 120 V of constant pressure at room temperature, with 3 % agarose gel electrophoresis 45 minutes, the EB dyeing of 0.5% μm of ol/L, gel Imaging system test sample, takes pictures；Photo shows that mealybug and standard DNA sample have clearly amplified band in the position 700bp, i.e., For target stripe；
The sequencing of S5, RCR product obtains gene order；
S6, Pseudococcidae insect genes compare
The sequence that mealybug to be measured is sequenced is compared with genetic barcode sequence disk Plays gene sequence of barcodes, if than To similarity 100%, then assert it for the type；When up to 100%, then carrying out amino not with any standard bar code sequence similarity The comparison of sour genetic distance determines whether in target zone.
4. a kind of detection method of Pseudococcidae insect genes barcode detection kit as claimed in claim 3, feature exist In the PCR reaction system is 50 μ L, wherein containing KCl, 2mmol/L MgCl containing 5 μ 10 × Taq of L buffer2、200µ Mol/L dNTP mix, 2U Taq DNA polymerase, 3 μ L DNA profilings, each 1 μm of ol/L of primer；
The PCR reaction system is 25 μ L, wherein 2 μ L of template DNA, contains Mg2+10 × reaction buffer 2.5 μ L, dNTPs 2 μ L, 5pmol/L upstream primer of 0.2mmol/L and each 1 μ L of downstream primer, 1.0 U Taq archaeal dna polymerase, 0.2 μ L, sterile water It mends to 25 μ L.
5. a kind of detection method of Pseudococcidae insect genes barcode detection kit as claimed in claim 3, feature exist In the sample in the S1 is the clean mealybug of pineapple, Lee's Bi Lishi ash mealybug, grey mealybug belong to a kind ofDysmicoccus neobrevipes, duckweed silk floss mealybug, Phenacoccus it is a kind ofPhenacoccus avenae, Phenacoccus it is a kind ofPhenacoccus parvus, short-tube lycoris silk floss mealybug, Japan silk floss mealybug, tangerine stern line mealybug, stern line mealybug belong to a kind ofPlanococcus ficus, Japan Stern line mealybug, stern line mealybug belong to a kind ofPlanococcus lilacinus, stern line mealybug belong to a kind ofPlanococcus minor、 Stern line mealybug belongs to a kind ofPlanococcus vovae, mealybug belong to a kind ofPseudococcus baliteus, sugarcane root mealybug, Kang Shi Mealybug, mealybug belong to a kind ofPseudococcus cryptus, Jack Bei Ershi mealybug, quasi- long tailed mealybug, mealybug belong to a kind ofPseudococcus viburni。
6. a kind of detection method of Pseudococcidae insect genes barcode detection kit as claimed in claim 3, feature exist In, the S6 Pseudococcidae insect genes comparison result includes the following steps,
S61, when obtained sequence and standard gene sequence of barcodes similarity 100%, then assert it for the type；
S62, when up to 100%, then pressing mealybug gene order to be measured not with any standard bar code sequence similarity, " no vertebra is dynamic Object codon " is translated as amino acid, uses " P-distance mould with amino acid sequence corresponding to standard gene sequence of barcodes Type " calculates genetic distance, if the standard gene bar code type with unknown type mealybug gene order genetic distance less than 1%, as Targeted species；If genetic distance is both greater than 1%, determine unknown mealybug type not within the scope of this 21 kinds of mealybugs.
CN201610381928.8A 2016-06-01 2016-06-01 A kind of Pseudococcidae insect genes barcode detection kit and its detection method Expired - Fee Related CN105925697B (en)

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CN106636411A (en) * 2016-12-28 2017-05-10 中华人民共和国连云港出入境检验检疫局 Xyleborus sp insect gene barcode detection kit and detection method thereof
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