CN105925265A - Polycyclic aromatic hydrocarbon degrading complete-set product composed of alfalfa and fungicide and application of complete-set product - Google Patents
Polycyclic aromatic hydrocarbon degrading complete-set product composed of alfalfa and fungicide and application of complete-set product Download PDFInfo
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Abstract
The invention discloses a polycyclic aromatic hydrocarbon degrading complete-set product composed of alfalfa and a fungicide and application of the complete-set product. The polycyclic aromatic hydrocarbon degrading complete-set product composed of the alfalfa and the fungicide is composed of the alfalfa and the fungicide, and the active ingredient of the fungicide is rhizobium petrolearium ACCC No.05789. The polycyclic aromatic hydrocarbon degrading complete-set product composed of the alfalfa and the fungicide can efficiently degrade polycyclic aromatic hydrocarbon in soil, and efficient, rapid and safe repairing of the soil contaminated by polycyclic aromatic hydrocarbon is achieved.
Description
Technical field
The present invention relates to the degrading polycyclic aromatic hydrocarbons that Herba Medicaginis and microbial inoculum are constituted in technical field of biological remediation complete sets of products and
Application.
Background technology
Polycyclic aromatic hydrocarbon (Polycyclic aromatic hydrocarbon, PAHs) refers to by two or more benzene
The class persistence organic pollutant that ring is fused together in a certain way, is mainly derived from mankind's activity and utilization of energy
Process.PAHs the most generally exists, and has high toxicity, heat stability, bioconcentration, hydrophobicity, no
The feature such as degradable, exists for a long time in natural environment, and is easily enriched with in vivo, to human health and life
State environment brings the biggest harm；In 1976,16 kinds of polycyclic aromatic hydrocarbons are classified as priority acccess control for this EPA
Pollutant list, 16 kinds of polycyclic aromatic hydrocarbons be respectively as follows: naphthalene (Nap), acenaphthylene (Acy), acenaphthene (Ace), fluorenes (FI), luxuriant and rich with fragrance (Phe),
Anthracene (An), fluoranthene (Flu), pyrene (Pyr), benzo [a] anthracene (BaA), (Chr), benzo [b] fluoranthene (BbF), benzene in the wrong
And [k] fluoranthene (BKF), benzo [a] pyrene (BaP), indeno [1,2,3-cd] pyrene (Inp), dibenzo [a, h] anthracene (DBA)
With benzo [ghi] (BgP).PAHs can be divided into low ring (phenyl ring<4) and epipodium (phenyl ring>=4) PAHs according to phenyl ring quantity
Two classes, and research find, along with the increase of phenyl ring number, its toxicity is the highest, and bioavailability is the poorest, be more difficult to by
Degraded.The PAHs in the mode Environment control of efficient, safety and environmental sound is how utilized to pollute, it has also become current
The emphasis of Research of Environmental Sciences's work.
Natural environment can be caused directly pollution by discharging waste gas, waste water and waste residue by PAHs polluter.PAHs is respectively
In individual medium, (air, water body and soil) all has distribution, wherein based on soil；Soil is that all multi-pollutants are the most main
The circulation home to return to wanted, there are about the PAHs of 90% and is distributed in soil in environment.PAHs mono-aspect by factory's disposal of pollutants,
The approach such as the leakage of petroleum product are directly entered in soil, and monitoring shows that this annual whole world of only oil pollution just has
8000000 tons of oil enter environment, and wherein China the most just has nearly 600,000 tons, and wherein major part is land oil,
Cause the pollution of soil；On the other hand, owing to PAHs has poorly water-soluble, the feature that partition coefficient is high, water body and air
In PAHs be prone to be assigned in airborne particulate surface, organism, in sedimentary, and then by ways such as sedimentation and absorption
Footpath enters soil；In addition, the sewage irrigation of agricultural land soil and the use of agriculture chemical cause soil pollution
Another major way.Therefore, soil is the main home to return to of the PAHs in environment, and PAHs has persistency with remote in addition
Distance translation, so either industrial occupancy is also that the soil of countries and regions of non-industrial occupancy the most generally exists many cyclophanes
Hydrocarbon pollutes, and the emphasis repairing polycyclic aromatic hydrocarbon is exactly the PAHs pollution of rehabilitating soil.
Summary of the invention
The technical problem to be solved is the polycyclic aromatic hydrocarbon in soil of how degrading.
For solving above-mentioned technical problem, the invention provides the complete sets of products of degrading polycyclic aromatic hydrocarbons, this complete sets of products can have
Effect degrading polycyclic aromatic hydrocarbons.
The complete sets of products of a kind of degrading polycyclic aromatic hydrocarbons provided by the present invention, is made up of Herba Medicaginis and microbial inoculum；Described microbial inoculum
Active component can be oil root nodule bacteria.
Herba Medicaginis in the complete sets of products of described degrading polycyclic aromatic hydrocarbons and microbial inoculum can independent packagings, it is possible to hybrid packed.
In the complete sets of products of above-mentioned degrading polycyclic aromatic hydrocarbons, described Herba Medicaginis can be alfalfa.
In the complete sets of products of above-mentioned degrading polycyclic aromatic hydrocarbons, described Herba Medicaginis can be the propagating materials of Herba Medicaginis, such as the seed of Herba Medicaginis.
In the complete sets of products of above-mentioned degrading polycyclic aromatic hydrocarbons, described oil root nodule bacteria concretely protect at Chinese microorganism strain
Hide administration committee's agricultural microorganism center (Chinese agriculture Microbiological Culture Collection administrative center, Agricultural
Culture Collection of China, is called for short ACCC, address: ZhongGuanCun south Street, Haidian District, BeiJing City 12)
The oil root nodule bacteria (Rhizobium petrolearium) of numbered ACCC 05789.
In the complete sets of products of above-mentioned degrading polycyclic aromatic hydrocarbons, the proportioning of described alfalfa seed and described microbial inoculum can be alfalfa seed
1000: (1.5-2.5) × 1011Oil root nodule bacteria described in CFU, the germination percentage of described alfalfa seed is in terms of 85%.
In one embodiment of the invention, the most described alfalfa seed of the proportioning of described alfalfa seed and described microbial inoculum
1000: described oil root nodule bacteria 2.5 × 1011CFU。
In the complete sets of products of above-mentioned degrading polycyclic aromatic hydrocarbons, described microbial inoculum can be described oil root nodule bacteria (Rhizobium
Petrolearium) after fermented cultivation, or directly use or concentrate the viable bacteria system using or making through carrier adsorption
Product.
In the complete sets of products of above-mentioned degrading polycyclic aromatic hydrocarbons, the dosage form of described microbial inoculum can be multiple dosage form, as liquor, Emulsion,
Suspending agent, powder, granule, wettable powder or water dispersible granules.
Above-mentioned microbial inoculum may also include carrier.Described carrier can be solid carrier and/or liquid-carrier.Described solid carrier is
Mineral material, biomaterial and/or macromolecular compound；Described mineral material can be Vermiculitum, clay, Talcum, kaolinite
At least one in soil, montmorillonite, white carbon, zeolite, Silicon stone, turfy soil and kieselguhr；Described biomaterial is each
In the feces of the straw of class crop, pine shell, Caulis et Folium Oryzae, Pericarppium arachidis hypogaeae, Semen Maydis powder, Semen Glycines powder, starch, the peat composed of rotten mosses and animal
At least one；Described macromolecular compound is polyvinyl alcohol and/or polyglycols.Described liquid-carrier can be organic solvent,
Vegetable oil, mineral oil or water；Described organic solvent is decane and/or dodecane.Described clay concretely attapulgite glues
Soil.Described attapulgite clay is a kind of clay mineral with attapulgite as main component, and main chemical compositions is hydration
Zeopan, containing elements such as K, Na, Ca, Fe, Al, Mg, Mn, Ti, have uniqueness dispersibility and
Absorbability.
In the complete sets of products of above-mentioned degrading polycyclic aromatic hydrocarbons, described polycyclic aromatic hydrocarbon can be naphthalene, acenaphthylene, acenaphthene, fluorenes, phenanthrene, anthracene,
Fluoranthene, pyrene, benzo [a] anthracene, dibenzo [a, h] anthracene, in the wrong, benzo [b] fluoranthene, benzo [k] fluoranthene, benzo [a]
At least one in pyrene, indeno [1,2,3-cd] pyrene and benzo [ghi].
The application in degrading polycyclic aromatic hydrocarbons of the complete sets of products of above-mentioned degrading polycyclic aromatic hydrocarbons falls within the protection model of the present invention
Enclose.
In the application of above-mentioned degrading polycyclic aromatic hydrocarbons, described degrading polycyclic aromatic hydrocarbons can be the polycyclic aromatic hydrocarbon in degraded soil.
In the application of above-mentioned degrading polycyclic aromatic hydrocarbons, described polycyclic aromatic hydrocarbon can be naphthalene, acenaphthylene, acenaphthene, fluorenes, phenanthrene, anthracene, glimmering
Anthracene, pyrene, benzo [a] anthracene, dibenzo [a, h] anthracene, bend, benzo [b] fluoranthene, benzo [k] fluoranthene, benzo [a] pyrene,
At least one in indeno [1,2,3-cd] pyrene and benzo [ghi].
A kind of reparation that the present invention also provides for is by the method for polycyclic aromatic hydrocarbon pollution.
A kind of reparation provided by the present invention is by the method for polycyclic aromatic hydrocarbon pollution, including by institute in above-mentioned complete sets of products
State alfalfa cultivation in by polycyclic aromatic hydrocarbons contaminated soil, described microbial inoculum is inoculated in described by polycyclic aromatic hydrocarbons contaminated soil
In earth, repaired by polycyclic aromatic hydrocarbons contaminated soil described.After reparation, the content of PAHs in soil is less than just
Beginning soil.
Above-mentioned reparation is by the method for polycyclic aromatic hydrocarbon pollution, and the inoculum concentration of described microbial inoculum is every square metre and accesses 25
×1010CFU oil root nodule bacteria ACCC 05789, described Herba Medicaginis is broadcast by the application rate of every square metre of sowing 1000
Kind.
Above-mentioned reparation by the method for polycyclic aromatic hydrocarbon pollution, described polycyclic aromatic hydrocarbon be naphthalene, acenaphthylene, acenaphthene, fluorenes, phenanthrene,
Anthracene, fluoranthene, pyrene, benzo [a] anthracene, in the wrong, benzo [b] fluoranthene, benzo [k] fluoranthene, benzo [a] pyrene, indeno [1,2,3-cd]
At least one in pyrene and benzo [ghi].
Above-mentioned reparation is by the method for polycyclic aromatic hydrocarbon pollution, and described Herba Medicaginis can be alfalfa.
Above-mentioned reparation is by the method for polycyclic aromatic hydrocarbon pollution, and described Herba Medicaginis can be the propagating materials of Herba Medicaginis, such as Herba Medicaginis
Seed or underground rhizome.
The complete sets of products utilizing the Herba Medicaginis of the present invention and the degrading polycyclic aromatic hydrocarbons of microbial inoculum composition processes by PAHs contaminated soil
After 60d, 2 rings PAHs degradation rate (clearance) are 28.74%；3 ring PAHs degradation rates are 8.28%-39.09%,
Average is 24.65%；4 ring PAHs degradation rates are 8.26%-19.54%, and average is 15.12%；5 ring PAHs degradeds
Rate is 6.5%-17.05%, and average is 10.72%；6 ring PAHs degradation rates are 5.34%-7.6%, and average is 6.47%；
After 90d, 2 ring PAHs degradation rates are 32.88%；3 ring PAHs degradation rates are 17.04%-36.92%, and average is
31.56%；4 ring PAHs degradation rates are 24.64%-30.65%, and average is 27.44%；5 ring PAHs degradation rates are
8.25%-44.28%, average is 26.56%；6 ring PAHs degradation rates are 29.52%-30.7%, and average is 30.11%.
Herba Medicaginis+bacterium treatment region (complete sets of products using the Herba Medicaginis of the present invention and the degrading polycyclic aromatic hydrocarbons of microbial inoculum composition processes)
It is 2.05 times of bacterium treatment region (using described microbial inoculum to process) to the total degradation rate of PAHs, is Herba Medicaginis treatment region
22.22 times of (using described Herba Medicaginis to process).Herba Medicaginis+bacterium treatment region to naphthalene, anthracene, phenanthrene, fluorenes, fluoranthene, pyrene,
The clearance (degradation rate) of benzo [a] pyrene (BaP) and benzo [k] fluoranthene (BKF) is obviously higher than bacterium treatment region and lucerne
Mu treatment region.The complete sets of products of the degrading polycyclic aromatic hydrocarbons that the Herba Medicaginis of the present invention and microbial inoculum are constituted can be in efficient degradation soil
Polycyclic aromatic hydrocarbon, it is achieved efficient, quick, the safety reparation that soil is polycyclic aromatic hydrocarbons contaminated.
Accompanying drawing explanation
Fig. 1 be Herba Medicaginis in different disposal soil plant height (unit: cm) (a), fresh weight (unit: g/ strain) (b),
Dry weight (unit: g/ strain) (c) and branch amount (unit: individual branch/strain) (d).
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is further described in detail, the embodiment be given only for
Illustrate the present invention rather than in order to limit the scope of the present invention.
Experimental technique in following embodiment, if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, the most commercially obtain.
Oil root nodule bacteria (Rhizobium petrolearium) ACCC No.05789 (oil in following embodiment
Root nodule bacteria (Rhizobium petrolearium) ACCC 05789) on April 10th, 2015 be concealed in China micro-
ACCC, address: Zhongguancun South St., Haidian District, Beijing City (are called for short in biological inoculum preservation administration committee's agricultural microorganism center
Street 12, INST OF AGRICULTURAL RESOURCES, postcode 100081), from this collection day
Play the public and can obtain this bacterial strain from China Committee for Culture Collection of Microorganisms's agricultural microorganism center.Oil root nodule bacteria
(Rhizobium petrolearium) ACCC No.05789 is hereinafter called for short oil root nodule bacteria ACCC 05789.
The clearance (degradation rate) of the PAHs in following embodiment=(comparison PAHs content-process PAHs content)
/ comparison PAHs content * 100%.
Embodiment 1, the degrading polycyclic aromatic hydrocarbons being made up of alfalfa and ACCC 05789 complete sets of products to many in soil
The degraded of PAH
One, pilot region
Test farmland is positioned near power plant, Guye District, Tangshan, Hebei Province, and there is the iron and steel that many places are large-scale region, farmland
Factory, power plant, coke-oven plant and Lime Factory etc., discharge substantial amounts of dusty gas throughout the year, and it is dirty that this area belongs to domestic air
Contaminate one of the most serious region.
Two, experimental design and enforcement
1, the preparation of oil root nodule bacteria ACCC 05789 microbial inoculum
By Vermiculitum 121 DEG C of sterilizing 100min in high-pressure steam sterilizing pan, go out altogether twice, between twice sterilizing, be spaced one
My god, it is put in aseptic stand-by after sterilizing, obtains sterile vermiculite.
Oil root nodule bacteria ACCC 05789 accesses 300mL TSB fluid medium, and (solute and concentration thereof is: cheese egg
White trypsinization thing 15g/L, Semen sojae atricolor papain digestion thing 5g/L, NaCl 5g/L；Solvent is distilled water；pH7.5)
Middle cultivation, under the conditions of 30 DEG C of 160r/min, 3d is cultivated in shaking table concussion, collects thalline, mixes with sterile vermiculite,
Obtain oil root nodule bacteria ACCC 05789 microbial inoculum.Oil root nodule bacteria ACCC in oil root nodule bacteria ACCC 05789 microbial inoculum
05789 content is 1010CFU/g。
2, the process of alfalfa seed
The mass of 1000 kernel of this alfalfa seed is 2.3 grams, and germination percentage is 85%.Utilize the hydrogen peroxide of 10% by lucerne before sowing
Mu seed disinfection about 5min, more repeatedly rinse with sterilized water, obtain the alfalfa seed of surface sterilizing.
3, experimental design and enforcement
Test agricultural land soil background PAHs content detection: sowing 2015 days JIUYUE 28, drills through test farmland table
The soil sample of following about the 20cm of layer, takes back laboratory, in taking-up part soil sample analysis soil originally after on-the-spot mix homogeneously
The PAHs content at the end, residue soil is put into-80 DEG C and is saved backup.
This test uses randomized block design, is randomly provided 4 treatment regions, and each treatment region sets three repetitions.4
Treatment region be respectively CK treatment region (not planting plant, do not connect root nodule bacteria, blank), bacterium treatment region (do not plant plant,
Connect root nodule bacteria), (kind Herba Medicaginis also connects root nodule for Herba Medicaginis treatment region (plant Herba Medicaginis, do not connect root nodule bacteria) and Herba Medicaginis+bacterium treatment region
Bacterium).
Study area is divided into 12 communities, and each plot area is 6 × 2m2.And use the random mode drawn lots to divide
Each process of cloth.Aforementioned four treatment region, except whether inoculating oil root nodule bacteria ACCC 05789 and whether in addition to Planting Medicago sativa,
Other operation is the most identical.CK treatment region neither inoculates oil root nodule bacteria ACCC 05789, the most not Planting Medicago sativa.Bacterium processes
District, JIUYUE in 2015 28 days is by oil root nodule bacteria ACCC 05789 microbial inoculum (10 of step 110CFU/g) according to the least
During the amount of district 300g is manured into soil, the content of oil root nodule bacteria ACCC 05789 in soil is made to reach 2.5 × 1011CFU
/m2.Herba Medicaginis treatment region, within 2015, the alfalfa seed of surface sterilizing is sown in soil by JIUYUE on the 28th, by often putting down
Side's rice is sowed the application rate of 1000 and is sowed.Herba Medicaginis+bacterium treatment region: JIUYUE in 2015 28 days is by step 1
Oil root nodule bacteria ACCC 05789 microbial inoculum (1010CFU/g), in being manured into soil according to the amount of every community 300g, soil is made
The content of middle oil root nodule bacteria ACCC 05789 reaches 2.5 × 1011CFU/m2；Within 2015, gone out in surface by JIUYUE on the 28th
The alfalfa seed of bacterium is sown in soil, sows by the application rate of every square metre of sowing 1000；Alfalfa
The proportioning of seed and oil root nodule bacteria ACCC 05789 is alfalfa seed 1000: 2.5 × 1011CFU oil root
Tumor bacterium ACCC 05789.After each community carries out above-mentioned process, in sowing the 60th day in the future (November 28 in 2015)
Regular sampling analysis, samples with five point sampling methods.For alfalfa plants and the collection of Rhizosphere Soil, dig with sampling spade
Go out band and take root in thing (degree of depth about 20cm), shake off the soil that root is loose, then remaining soil is put into sampling with plant
Take back laboratory in Dai, and when gathering CK treatment region and bacterium treatment region soil, randomly select 5 points, carry after on-the-spot mixing
Return laboratory.
4, sample treatment analysis
The mensuration of 4.1 plant physical signs
The alfalfa plants sample collected, each community is chosen 15 strains and is analyzed processing, first with the abundant drip washing of distilled water
The earth on surface, then the moisture of plant surface is blotted with filter paper, calculate the overall weight in wet base of 15 strain Herba Medicaginiss, and independent measurement
Every plant height of strain Herba Medicaginis, branch amount and dross number；Plant is put in baking oven after terminating and dries to constant weight by measurement, surveys
Its overall dry weight.
The detection of 4.2 PAHs in soils
The method used is referring especially to EPA3550C standard (USEPA, 2000.Method 3550 C, Test Methods
for Evaluating Solid Waste.US EPA SW-846,third ed.US Environmental
Protection Agency, Washington, DC), it is analysed to soil sample after drying grinding, sieves (60 mesh),
Weigh appropriate amount of sample and fill in glass centrifuge tube in 50ml tool, add 30mL dichloromethane/normal hexane (1:1, v/v)
Mixed solvent, is placed in ultrasonic cleaner concussion and extracts 1h, extracts after terminating, retains supernatant, again add
The mixed solvent of 30ml dichloromethane/normal hexane (1:1, v/v), shakes ultrasonic 30min, and later process is carried out altogether
Twice.In order to avoid the rising of water temperature, change a water every 30min.By the supernatant collected at rotary evaporation
Device is concentrated into about 1-2mL, is subsequently adding 8-10mL normal hexane, continue to be concentrated into about 3mL, to reach molten
The purpose that agent is replaced.
The concentrated solution of above-mentioned gained is added silica gel/aluminium oxide/anhydrous sodium sulfate chromatographic column (orlop 12cm is silica gel,
Middle level 6cm is neutral alumina, and top is 1cm anhydrous sodium sulfate) carry out stage purification, it is subsequently adding 15mL
Normal hexane rinse chromatographic column, elute alkane component and discard, add 70mL dichloromethane/normal hexane mixed liquor (3:7,
V/v) elute alkane and aromatic hydrocarbons (polycyclic aromatic hydrocarbon and organochlorine), then above-mentioned eluent is concentrated on a rotary evaporator
To below 1mL, utilize chromatographic grade normal hexane to be settled to 1mL, load and brown sample bottle is delivered to Ministry of Land and Resources guarantor
Determine mineral resources supervisory detection center carry out detection analyze (utilize GC-MS analyze 16 kinds of PAHs: naphthalenes (Nap), acenaphthylenes
(Acy), acenaphthene (Ace), fluorenes (FI), luxuriant and rich with fragrance (Phe), anthracene (An), fluoranthene (Flu), pyrene (Pyr), benzo [a] anthracene (BaA),
Bend (Chr), benzo [b] fluoranthene (BbF), benzo [k] fluoranthene (BKF), benzo [a] pyrene (BaP), indeno [1,2,3-cd]
Pyrene (Inp), dibenzo [a, h] anthracene (DBA) and benzo [ghi] (BgP).
5 results and analysis
5.1 study area soil background PAHs content analysis
As shown in Table 1, in the soil of study area, a point ring content order of background PAHs is: 2 rings < 3 rings < 6 rings < 5 rings < 4
Ring, accounting for background values PAHs total amount is 34.13%, 31.55%, 20.82%, 11.47%, 2.02% respectively；Visible soil
The content of middle epipodium PAHs is significantly larger than low ring PAHs content, and epipodium PAHs is more easy in soil accumulation, and is difficult to fall
Solve and remove；In soil, low ring PAHs is luxuriant and rich with fragrance and fluoranthene content respectively reaches 246 μ g/kg and 285 μ g/kg, and research is sent out
Existing, due to the luxuriant and rich with fragrance and specific structural features of fluoranthene, cause it all to contain in different surrounding mediums of a relatively high dense
Degree.
The content of soil background polycyclic aromatic hydrocarbon tested by table 1.
| Detectable substance | Content (μ g/kg) | Detectable substance | Content (μ g/kg) |
| Naphthalene (Nap) | 56.30±3.13 | Benzo [a] anthracene (BaA) | 199.50±2.51 |
| Acenaphthylene (Acy) | 13.77±1.24 | Bend (Chr) | 264.38±10.55 |
| Acenaphthene (Ace) | 6.54±0.14 | Benzo [b] fluoranthene (BbF) | 504.51±35.57 |
| Fluorenes (FI) | 21.37±0.82 | Benzo [k] fluoranthene (BKF) | 103.36±7.88 |
| Anthracene (An) | 31.4±6.50 | Benzo [a] pyrene (BaP) | 179.19±11.15 |
| Luxuriant and rich with fragrance (Phe) | 245.66±0.95 | Indeno [1,2,3-cd] pyrene (Inp) | 241.30±28.59 |
| Fluoranthene (Flu) | 284.68±5.13 | Dibenzo [a, h] anthracene (DBA) | 89.47±13.51 |
| Pyrene (Pyr) | 199.58±4.85 | Benzo [ghi] (BgP) | 337.24±37.12 |
5.2 Herba Medicaginis physical signs features
Relate to, in study area two, the plant that Herba Medicaginis processes and carried out following physical signs analysis, including plant height, fresh
Weight, dry weight and branch amount, result is as shown in Figure 1.After planting 60 days, alfalfa in the case of not inoculating microbial inoculum
Grow fine, illustrate that it has certain tolerance to the PAHs in contaminated soil.And Planting Medicago sativa connect bacterium
Plant height, fresh weight, dry weight and the branch amount processed all is higher than the process of only Planting Medicago sativa, it is seen that oil root nodule bacteria ACCC
05789 pair of Herba Medicaginis has certain growth-promoting functions；On the one hand be because oil root nodule bacteria ACCC 05789 can dross in
Alfalfa, and find that the root nodule number (average 46/strain) that the 60th day " Herba Medicaginis+bacterium " processes is wanted in this experiment
Far more than " Herba Medicaginis " the root nodule number (10) that individually cultivation processes；On the other hand, inoculation oil root nodule bacteria ACCC 05789
Promote the degraded of PAHs in contaminated soil, decrease its murder by poisoning to Herba Medicaginis, and then promote alfalfa growing.
5.3 Herba Medicaginiss and oil root nodule bacteria ACCC 05789 work in coordination with repairing effect
After 60d, in different disposal soil, PAHs content and clearance are as shown in table 2 and table 3；Planting Medicago sativa also connects
In the process of bacterium, PAHs clearance is more than singly connecing bacterium or the process of single Planting Medicago sativa, show " Herba Medicaginis <bacterium < Herba Medicaginis+bacterium "
Variation tendency；And in soil, the degradation rate of different number of rings PAHs there are differences: 2 ring PAHs degradation rates (are removed
Rate) it is 28.74%；3 ring PAHs degradation rates are 8.28%-39.09%, and average is 24.65%；4 ring PAHs degradation rates
For 8.26%-19.54%, average is 15.12%；5 ring PAHs degradation rates are 6.5%-17.05%, and average is 10.72%；
6 ring PAHs degradation rates are 5.34%-7.6%, and average is 6.47%.Visible along with the increase of phenyl ring number, PAHs's
Average degradation rate is gradually lowered, and the degradation effect of low ring PAHs is higher than epipodium PAHs.Herba Medicaginis+bacterium treatment region is to PAHs
Total degradation rate be 2.05 times of bacterium treatment region, be 22.22 times of Herba Medicaginis treatment region.Herba Medicaginis+bacterium treatment region to naphthalene,
The clearance (degradation rate) of anthracene, phenanthrene, fluorenes, fluoranthene, pyrene, benzo [a] pyrene (BaP) and benzo [k] fluoranthene (BKF)
Obviously higher than bacterium treatment region and Herba Medicaginis treatment region.
2. kinds of table plant 60 days after PAHs content (μ g/kg) in different disposal soil
The removal situation of PAHs in 60 days after stain soil planted by 3. kinds of table
In note: "-" represents negative value, i.e. this treatment region, PAH content is higher than CK treatment region.
Claims (10)
1. the complete sets of products of degrading polycyclic aromatic hydrocarbons, is made up of Herba Medicaginis and microbial inoculum；The active component of described microbial inoculum is oil
Root nodule bacteria.
The complete sets of products of degrading polycyclic aromatic hydrocarbons the most according to claim 1, it is characterised in that: described oil root
Tumor bacterium is oil root nodule bacteria (Rhizobium petrolearium) ACCC No.05789.
The complete sets of products of degrading polycyclic aromatic hydrocarbons the most according to claim 1 and 2, it is characterised in that: described lucerne
Mu is alfalfa.
4. according to the complete sets of products of described degrading polycyclic aromatic hydrocarbons arbitrary in claim 1-3, it is characterised in that: described
Polycyclic aromatic hydrocarbon is naphthalene, anthracene, phenanthrene, fluorenes, fluoranthene, pyrene, benzo [a] pyrene, benzo [k] fluoranthene, acenaphthylene, acenaphthene, benzo
[a] anthracene, in the wrong, benzo [b] fluoranthene, indeno [1,2,3-cd] pyrene, dibenzo [a, h] anthracene and benzo [ghi].
5. the complete sets of products of arbitrary described degrading polycyclic aromatic hydrocarbons application in degrading polycyclic aromatic hydrocarbons in claim 1-4.
Application the most according to claim 5, it is characterised in that: described degrading polycyclic aromatic hydrocarbons is in degraded soil
Polycyclic aromatic hydrocarbon.
7. according to the application described in claim 5 or 6, it is characterised in that: described polycyclic aromatic hydrocarbon be naphthalene, anthracene, phenanthrene,
Fluorenes, fluoranthene, pyrene, benzo [a] pyrene, benzo [k] fluoranthene, acenaphthylene, acenaphthene, benzo [a] anthracene, bend, benzo [b] fluoranthene,
At least one in indeno [1,2,3-cd] pyrene, dibenzo [a, h] anthracene and benzo [ghi].
8. repair by the method for polycyclic aromatic hydrocarbon pollution, including by described complete sets of products arbitrary in claim 1-4
Described in alfalfa cultivation in by polycyclic aromatic hydrocarbons contaminated soil, by described complete sets of products arbitrary in claim 1-4
Described microbial inoculum be inoculated in described by polycyclic aromatic hydrocarbons contaminated soil, carried out by polycyclic aromatic hydrocarbons contaminated soil described
Repair.
Method the most according to claim 8, it is characterised in that: above-mentioned reparation is by polycyclic aromatic hydrocarbon pollution
In method, the inoculum concentration of described microbial inoculum is every square metre of access (1.5-2.5) × 1011CFU oil root nodule bacteria ACCC
05789, described Herba Medicaginis is sowed by the application rate of every square metre of sowing 1000.
Method the most according to claim 8 or claim 9, it is characterised in that: described polycyclic aromatic hydrocarbon be naphthalene, anthracene, phenanthrene,
Fluorenes, fluoranthene, pyrene, benzo [a] pyrene, benzo [k] fluoranthene, acenaphthylene, acenaphthene, benzo [a] anthracene, bend, benzo [b] fluoranthene,
At least one in indeno [1,2,3-cd] pyrene, dibenzo [a, h] anthracene and benzo [ghi].
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| CN108911864A (en) * | 2018-08-17 | 2018-11-30 | 西北工业大学 | A kind of polycyclic aromatic hydrocarbon pollution repair materials and preparation method thereof |
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