CN105920172A - Brain aging resisting functional food and preparation method - Google Patents
Brain aging resisting functional food and preparation method Download PDFInfo
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- CN105920172A CN105920172A CN201610264125.4A CN201610264125A CN105920172A CN 105920172 A CN105920172 A CN 105920172A CN 201610264125 A CN201610264125 A CN 201610264125A CN 105920172 A CN105920172 A CN 105920172A
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- 235000013376 functional food Nutrition 0.000 title claims abstract description 64
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 210000004556 brain Anatomy 0.000 title abstract description 16
- 230000032683 aging Effects 0.000 title abstract description 12
- 239000000284 extract Substances 0.000 claims abstract description 52
- UAJTZZNRJCKXJN-UHFFFAOYSA-M sodium;2-dodecoxy-2-oxoethanesulfonate Chemical compound [Na+].CCCCCCCCCCCCOC(=O)CS([O-])(=O)=O UAJTZZNRJCKXJN-UHFFFAOYSA-M 0.000 claims abstract description 40
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 128
- 238000010828 elution Methods 0.000 claims description 36
- 238000002481 ethanol extraction Methods 0.000 claims description 36
- 239000007788 liquid Substances 0.000 claims description 32
- 239000000843 powder Substances 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 239000011347 resin Substances 0.000 claims description 22
- 229920005989 resin Polymers 0.000 claims description 22
- 238000002156 mixing Methods 0.000 claims description 21
- 238000000605 extraction Methods 0.000 claims description 18
- 238000010992 reflux Methods 0.000 claims description 18
- 238000011010 flushing procedure Methods 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 7
- 238000007873 sieving Methods 0.000 claims description 7
- 235000013305 food Nutrition 0.000 claims description 5
- 239000003463 adsorbent Substances 0.000 claims description 2
- 239000004615 ingredient Substances 0.000 abstract 2
- 239000000243 solution Substances 0.000 description 30
- 230000000694 effects Effects 0.000 description 18
- 241000699670 Mus sp. Species 0.000 description 15
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 9
- 102000016938 Catalase Human genes 0.000 description 8
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- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- -1 and during aging Proteins 0.000 description 3
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- 238000003304 gavage Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000010254 subcutaneous injection Methods 0.000 description 3
- 239000007929 subcutaneous injection Substances 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- GZCGUPFRVQAUEE-KCDKBNATSA-N aldehydo-D-galactose Chemical compound OC[C@@H](O)[C@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-KCDKBNATSA-N 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
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- 208000024806 Brain atrophy Diseases 0.000 description 1
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- 108010052832 Cytochromes Proteins 0.000 description 1
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
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- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical class [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000023852 carbohydrate metabolic process Effects 0.000 description 1
- 235000021256 carbohydrate metabolism Nutrition 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 239000000701 coagulant Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/78—Saururaceae (Lizard's-tail family)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a brain aging resisting functional food and a preparation method thereof. The functional food consists of a folium eriobotryae extract and a herba houttuyniae extract. The functional food provided by the invention can obviously delay brain aging, and the folium eriobotryae extract and the herba houttuyniae extract, which serve as main functional ingredients, are mixed at a ratio of (3-5) to 1 according to parts by weight. The preparation method of the functional food is simple and is easy to operate, and the functional food is uniform in ingredient. The functional food disclosed by the invention, compared with the prior art, has remarkable substantive features and significant progress.
Description
Technical field
The invention belongs to field of food, be specifically related to a kind of old functional food of anti senile and preparation method thereof.
Background technology
Except showing as the alleviating of brain weight, brain atrophy, neuron loss etc. in morphology in brain aging process, its biochemistry
Change and also have significant change such as the content of brain protein, the level of enzyme and activity etc..Research prove, the protein in human body with
Increasing age and be gradually reduced, protein is the specific form that cell exists, and is the important substance of vital movement, potein deficiency,
The function of cell is then decreased obviously, and the functional activity of the function of organism particularly brain is the most substantially failed, and enzyme is organism metabolism
One of main body, participates in the synthesis of protein, and during aging, enzyme level and enzymatic activity all occur significantly to change, and LDH is
Important enzyme in carbohydrate metabolism, participates in internal redox reaction, and oxidoreduction is one of important biochemical reaction of organism,
Multiple oxidoreductase such as LDH, glucose dehydrogenase, tiger amber acidohydrogenase, cytochrome oxidase, cytochrome reductase
Deng enzyme level and enzymatic activity all decline with the biological and increasing age of the mankind.Old people's substance metabolism and energy metabolism low SI
Reason may just be because the activity of these enzymes and reduce and caused.
Summary of the invention
It is an object of the invention to a kind of old functional food of anti senile and preparation method thereof, this functional food can significantly delay brain
Senescence process.
The above-mentioned purpose of the present invention is achieved by techniques below scheme:
A kind of old functional food of anti senile, including Folium Eriobotryae fermented product and Herba Houttuyniae extract；
Wherein, described Folium Eriobotryae fermented product is prepared via a method which:
Step S1, pulverizes dry Folium Eriobotryae；
Step S2, extracts with 75%～85% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, uses macroporous resin enrichment active component, first with 10～20% second
Alcohol rinses 5～9 column volumes and removes big polar components, then with 70～80% ethanol elution 12～16 column volumes, collection 70～80%
Ethanol elution, concentrating under reduced pressure, it is spray-dried；
Wherein, described Herba Houttuyniae extract is prepared via a method which:
Step S1, pulverizes dry Herba Houttuyniae；
Step S2, extracts with 70%～80% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, uses macroporous resin enrichment active component, first with 5～15% ethanol
Rinse 8～12 column volumes and remove big polar components, then with 75～85% ethanol elution 10～14 column volumes, collection 75～85%
Ethanol elution, concentrating under reduced pressure, it is spray-dried；
The weight ratio of described Folium Eriobotryae fermented product and Herba Houttuyniae extract is 3～5:1.
Further, described macroporous resin is food stage D101 macroporous adsorbent resin.
Further, described Folium Eriobotryae fermented product is prepared via a method which:
Step S1, pulverizes dry Folium Eriobotryae；
Step S2, extracts with 80% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 15%
7 column volumes of alcohol flushing remove big polar component, then with 14 column volumes of 75% ethanol elution, collect 75% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
Further, described Herba Houttuyniae extract is prepared via a method which:
Step S1, pulverizes dry Herba Houttuyniae；
Step S2, extracts with 75% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 10%
10 column volumes of alcohol flushing remove big polar component, then with 12 column volumes of 80% ethanol elution, collect 80% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
The preparation method of above-mentioned functions food, comprises the steps:
Step S1, obtains mixing fine powders by Folium Eriobotryae fermented product and Herba Houttuyniae extract mix homogeneously；
Step S2,110～120 DEG C of high temperature sterilizes, to dry, the water content of the mixing fine powders after drying is 3%～5%；
Step S3, grinds mixing fine powders, crosses 100 mesh sieves；
Step S4, the fine powder after sieving makes functional food.
Advantages of the present invention:
The functional food that the present invention provides can significantly slow brain aging, and main composition is Folium Eriobotryae fermented product and Herba Houttuyniae
Extract.The preparation method of this functional food is simple, and composition is uniform, easily operates.The present invention compared with prior art has prominent
The substantive distinguishing features gone out and significantly progress.
Detailed description of the invention
Further illustrate the essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.To the greatest extent
The present invention is explained in detail by pipe with reference to preferred embodiment, it will be understood by those within the art that, can be to the present invention
Technical scheme modify or equivalent, without deviating from the spirit and scope of technical solution of the present invention.
Embodiment 1: the preparation of functional food
Proportioning raw materials: the weight ratio of Folium Eriobotryae fermented product and Herba Houttuyniae extract is 4:1.
The preparation method of Folium Eriobotryae fermented product:
Step S1, pulverizes dry Folium Eriobotryae；
Step S2, extracts with 80% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 15%
7 column volumes of alcohol flushing remove big polar component, then with 14 column volumes of 75% ethanol elution, collect 75% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
The preparation method of Herba Houttuyniae extract:
Step S1, pulverizes dry Herba Houttuyniae；
Step S2, extracts with 75% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 10%
10 column volumes of alcohol flushing remove big polar component, then with 12 column volumes of 80% ethanol elution, collect 80% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
The preparation method of functional food:
Step S1, obtains mixing fine powders by Folium Eriobotryae fermented product and Herba Houttuyniae extract mix homogeneously；
Step S2,120 DEG C of high temperature sterilizes, to dry, the water content of the mixing fine powders after drying is 3%～5%；
Step S3, grinds mixing fine powders, crosses 100 mesh sieves；
Step S4, the fine powder after sieving makes functional food.
Embodiment 2: the preparation of functional food
Proportioning raw materials: the weight ratio of Folium Eriobotryae fermented product and Herba Houttuyniae extract is 3:1.
The preparation method of Folium Eriobotryae fermented product:
Step S1, pulverizes dry Folium Eriobotryae；
Step S2, extracts with 80% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 15%
7 column volumes of alcohol flushing remove big polar component, then with 14 column volumes of 75% ethanol elution, collect 75% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
The preparation method of Herba Houttuyniae extract:
Step S1, pulverizes dry Herba Houttuyniae；
Step S2, extracts with 75% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 10%
10 column volumes of alcohol flushing remove big polar component, then with 12 column volumes of 80% ethanol elution, collect 80% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
The preparation method of functional food:
Step S1, obtains mixing fine powders by Folium Eriobotryae fermented product and Herba Houttuyniae extract mix homogeneously；
Step S2,120 DEG C of high temperature sterilizes, to dry, the water content of the mixing fine powders after drying is 3%～5%；
Step S3, grinds mixing fine powders, crosses 100 mesh sieves；
Step S4, the fine powder after sieving makes functional food.
Embodiment 3: the preparation of functional food
Proportioning raw materials: the weight ratio of Folium Eriobotryae fermented product and Herba Houttuyniae extract is 5:1.
The preparation method of Folium Eriobotryae fermented product:
Step S1, pulverizes dry Folium Eriobotryae；
Step S2, extracts with 80% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 15%
7 column volumes of alcohol flushing remove big polar component, then with 14 column volumes of 75% ethanol elution, collect 75% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
The preparation method of Herba Houttuyniae extract:
Step S1, pulverizes dry Herba Houttuyniae；
Step S2, extracts with 75% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 10%
10 column volumes of alcohol flushing remove big polar component, then with 12 column volumes of 80% ethanol elution, collect 80% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
The preparation method of functional food:
Step S1, obtains mixing fine powders by Folium Eriobotryae fermented product and Herba Houttuyniae extract mix homogeneously；
Step S2,120 DEG C of high temperature sterilizes, to dry, the water content of the mixing fine powders after drying is 3%～5%；
Step S3, grinds mixing fine powders, crosses 100 mesh sieves；
Step S4, the fine powder after sieving makes functional food.
The weight ratio of embodiment 4: comparative example, Folium Eriobotryae fermented product and Herba Houttuyniae extract is 2:1
Proportioning raw materials: the weight ratio of Folium Eriobotryae fermented product and Herba Houttuyniae extract is 2:1.
The preparation method of Folium Eriobotryae fermented product:
Step S1, pulverizes dry Folium Eriobotryae；
Step S2, extracts with 80% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 15%
7 column volumes of alcohol flushing remove big polar component, then with 14 column volumes of 75% ethanol elution, collect 75% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
The preparation method of Herba Houttuyniae extract:
Step S1, pulverizes dry Herba Houttuyniae；
Step S2, extracts with 75% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 10%
10 column volumes of alcohol flushing remove big polar component, then with 12 column volumes of 80% ethanol elution, collect 80% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
The preparation method of functional food:
Step S1, obtains mixing fine powders by Folium Eriobotryae fermented product and Herba Houttuyniae extract mix homogeneously；
Step S2,120 DEG C of high temperature sterilizes, to dry, the water content of the mixing fine powders after drying is 3%～5%；
Step S3, grinds mixing fine powders, crosses 100 mesh sieves；
Step S4, the fine powder after sieving makes functional food.
The weight ratio of embodiment 5: comparative example, Folium Eriobotryae fermented product and Herba Houttuyniae extract is 6:1
Proportioning raw materials: the weight ratio of Folium Eriobotryae fermented product and Herba Houttuyniae extract is 6:1.
The preparation method of Folium Eriobotryae fermented product:
Step S1, pulverizes dry Folium Eriobotryae；
Step S2, extracts with 80% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 15%
7 column volumes of alcohol flushing remove big polar component, then with 14 column volumes of 75% ethanol elution, collect 75% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
The preparation method of Herba Houttuyniae extract:
Step S1, pulverizes dry Herba Houttuyniae；
Step S2, extracts with 75% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 10%
10 column volumes of alcohol flushing remove big polar component, then with 12 column volumes of 80% ethanol elution, collect 80% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
The preparation method of functional food:
Step S1, obtains mixing fine powders by Folium Eriobotryae fermented product and Herba Houttuyniae extract mix homogeneously；
Step S2,120 DEG C of high temperature sterilizes, to dry, the water content of the mixing fine powders after drying is 3%～5%；
Step S3, grinds mixing fine powders, crosses 100 mesh sieves；
Step S4, the fine powder after sieving makes functional food.
Embodiment 6: effect example
One, materials and methods
1.1 medicines and reagent: 1.～the most respectively according to embodiment 1～5 functional food is prepared, and is made into experiment desired concn with distilled water,
Be placed in 4 DEG C standby；SOD test kit is built up institute of biological products by Nanjing and provides；D-galactose is provided by Shanghai reagent two factory.
1.2 laboratory animals: Kunming mouse, body weight (20 ± 2) g, Anhui Province's medical test provided.
1.3 experimental techniques: mice 70, male and female half and half, body weight (20 ± 2) g, mice is randomly divided into 7 groups, often organizes 10
Only, i.e. Normal group, D-gal Aging model group, functional food 1.～5. group.In addition to Normal group, other 6 groups are moved
Thing nape subcutaneous injection equal every day 5%D-galactose 0.5mL, normal group mice then nape subcutaneous injection normal saline 0.5mL.
In the 10d of injection of d-galactose, functional food 1.～5. treated animal gavage respectively gives functional food 1.～5. 200mg kg-1,
Normal group mice and aging model treated animal then gavage every day equal-volume normal saline.Continuous use 30d.Last day
After gavage and injection of d-galactose 2h, eye socket takes blood, and anticoagulant heparin (4 DEG C of preservations) is lipid peroxide (LPO) in serum
The mensuration of content, then sacrificed by decapitation animal, take out cerebral tissue and make homogenate for catalase (CAT), superoxides discrimination
Change enzyme (SOD), the assay of peroxidase (POD).
Two, result
2.1 functional food are on the impact-ultraviolet spectrophotometry of CAT activity in brain aging mice serum
Table 1 shows, under the effect of D-galactose, the CAT activity in model group animal brain is remarkably decreased, and normally
Matched group is compared, and difference is the most notable.Compared with model group, in the mouse blood that 1.～3. functional food is organized, CAT activity is substantially
Rise (P < 0.01)；Compared with model group, in the mouse blood that 4.～5. functional food is organized, CAT activity is without substantially rising (P ＞
0.05).Note: compare * P < 0.05, * * P < 0.01 with model group.
Table 1 functional food is on the impact (X ± SD) of CAT activity in mice serum
| Group | Protein content/mg mL-1 | CAT(k/gHb) |
| Normal group | 0.75 | 81.29±6.73 |
| Model group | 0.71 | 74.24±7.38 |
| Functional food 1. group | 0.70 | 96.93±8.26** |
| Functional food 2. group | 0.69 | 94.89±8.23** |
| Functional food 3. group | 0.70 | 95.85±8.08** |
| Functional food 4. group | 0.68 | 76.32±9.06 |
| Functional food 5. group | 0.69 | 77.53±8.11 |
2.2 functional food are on the impact of LPO in brain aging Mice brain tissues-tissue homogenate MDA colorimetry
Table 2 shows, continuous subcutaneous injection 5% galactose aqueous solution, and the LPO content in Mice brain tissues is apparently higher than the most right
According to group, illustrate that D-galactose can make cell membrane generation lipid peroxidation, and 1.～3. functional food can make in Mice brain tissues
LPO content decline, with model group comparing difference substantially (P < 0.01), point out it to have the effect of anti-lipid peroxidation；Merit
Food 4.～5. group the LPO content in Mice brain tissues can not be made to be decreased obviously, (P ＞ inconspicuous with model group comparing difference
0.05).Note: compare * P < 0.05, * * P < 0.01 with model group.
Table 2 functional food is on the impact (X ± SD) of LPO in Mice brain tissues
| Group | MDA(nmoL/mgprot) |
| Normal group | 1.18±0.19 |
| Model group | 1.24±0.15 |
| Functional food 1. group | 0.91±0.16** |
| Functional food 2. group | 0.92±0.14** |
| Functional food 3. group | 0.93±0.13** |
| Functional food 4. group | 1.18±0.15 |
| Functional food 5. group | 1.19±0.14 |
2.3 functional food are on the impact-photo-reduction NBT method of SOD activity in brain aging Mice brain tissues
Table 3 shows, the SOD activity in model group animal brain is remarkably decreased, and compared with Normal group, difference is the most aobvious
Write.Compared with model group, in the Mice brain tissues that 1.～3. functional food is organized, SOD activity substantially rises (P < 0.01)；With model
Group is compared, and in the Mice brain tissues that 4.～5. functional food is organized, SOD activity is without substantially rising (P ＞ 0.05).Note: with model group
Relatively * P < 0.05, * * P < 0.01.
Table 3 is on the impact of SOD activity in brain aging Mice brain tissues
| Group | SOD vigor (μ/mgprot) |
| Normal group | 5.48±1.41 |
| Model group | 3.61±1.43 |
| Functional food 1. group | 6.36±1.39** |
| Functional food 2. group | 6.35±1.38** |
| Functional food 3. group | 6.35±1.35** |
| Functional food 4. group | 3.85±1.38 |
| Functional food 5. group | 3.83±1.40 |
Above-mentioned test result indicate that, the functional food of the present invention has the effect that anti senile is old, and this effect is its functional component Folium Eriobotryae
The result that leaf extract and Herba Houttuyniae extract play a role jointly, and weight ratio is required to be 3～5:1.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but does not limit protection scope of the present invention with this.
It will be understood by those within the art that, technical scheme can be modified or equivalent, and not take off
Essence and protection domain from technical solution of the present invention.
Claims (5)
1. the old functional food of anti senile, it is characterised in that: include Folium Eriobotryae fermented product and Herba Houttuyniae extract；
Wherein, described Folium Eriobotryae fermented product is prepared via a method which:
Step S1, pulverizes dry Folium Eriobotryae；
Step S2, extracts with 75%～85% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, uses macroporous resin enrichment active component, first with 10～20% second
Alcohol rinses 5～9 column volumes and removes big polar components, then with 70～80% ethanol elution 12～16 column volumes, collection 70～80%
Ethanol elution, concentrating under reduced pressure, it is spray-dried；
Wherein, described Herba Houttuyniae extract is prepared via a method which:
Step S1, pulverizes dry Herba Houttuyniae；
Step S2, extracts with 70%～80% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, uses macroporous resin enrichment active component, first with 5～15% ethanol
Rinse 8～12 column volumes and remove big polar components, then with 75～85% ethanol elution 10～14 column volumes, collection 75～85%
Ethanol elution, concentrating under reduced pressure, it is spray-dried；
The weight ratio of described Folium Eriobotryae fermented product and Herba Houttuyniae extract is 3～5:1.
The old functional food of anti senile the most according to claim 1, it is characterised in that: described macroporous resin is food stage D101
Macroporous adsorbent resin.
The old functional food of anti senile the most according to claim 2, it is characterised in that described Folium Eriobotryae fermented product is by as follows
Prepared by method:
Step S1, pulverizes dry Folium Eriobotryae；
Step S2, extracts with 80% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 15%
7 column volumes of alcohol flushing remove big polar component, then with 14 column volumes of 75% ethanol elution, collect 75% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
The old functional food of anti senile the most according to claim 2, it is characterised in that described Herba Houttuyniae extract is by as follows
Prepared by method:
Step S1, pulverizes dry Herba Houttuyniae；
Step S2, extracts with 75% alcohol heat reflux, united extraction liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste；
Step S3, ethanol extraction concentrated solution dilute with water, filters, with D101 macroporous resin enrichment active component, first with 10%
10 column volumes of alcohol flushing remove big polar component, then with 12 column volumes of 80% ethanol elution, collect 80% ethanol elution
Liquid, concentrating under reduced pressure, it is spray-dried.
5. the preparation method of the arbitrary described functional food of Claims 1 to 4, it is characterised in that comprise the steps:
Step S1, obtains mixing fine powders by Folium Eriobotryae fermented product and Herba Houttuyniae extract mix homogeneously；
Step S2,110～120 DEG C of high temperature sterilizes, to dry, the water content of the mixing fine powders after drying is 3%～5%；
Step S3, grinds mixing fine powders, crosses 100 mesh sieves；
Step S4, the fine powder after sieving makes functional food.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1586242A (en) * | 2004-08-19 | 2005-03-02 | 洪伟 | Health tea of cordate houttuynia |
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2016
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1586242A (en) * | 2004-08-19 | 2005-03-02 | 洪伟 | Health tea of cordate houttuynia |
Non-Patent Citations (1)
| Title |
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| 柯仲成等: "枇杷叶总黄酮提取工艺的优化", 《食品与发酵工业》 * |
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