CN105920145A - Preparation method of liver-smoothing injection preparation - Google Patents
Preparation method of liver-smoothing injection preparation Download PDFInfo
- Publication number
- CN105920145A CN105920145A CN201610338254.3A CN201610338254A CN105920145A CN 105920145 A CN105920145 A CN 105920145A CN 201610338254 A CN201610338254 A CN 201610338254A CN 105920145 A CN105920145 A CN 105920145A
- Authority
- CN
- China
- Prior art keywords
- extract
- preparation
- filter
- water
- adds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/744—Gardenia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Dermatology (AREA)
- Molecular Biology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method of a liver-soothing injection preparation. The liver-soothing injection preparation is mainly prepared from a herba artemisiae scopariae extract, a fructus gardeniae extract, baicalin, a radix isatidis extract and a ganoderma extract; by means of the preparation method of the liver-soothing injection preparation, the specific process and parameters are improved, the effective component indigo in radix isatidis can be detected easily, and the content of detected indigo is high. The drug efficacy of the liver-soothing injection preparation can be effectively improved.
Description
Technical field
The invention belongs to the field of Chinese medicines, be specifically related to the preparation method of the peaceful ejection preparation of a kind of soothing liver-QI.
Background technology
Chinese medicine extraction is a kind of method conventional in herbal pharmaceutical, and chemical composition of Chinese materia medica is extremely complex, need to be from multiple
Miscellaneous mixture extracts active ingredient of Chinese herbs effective to human body.To improve drug effect.
Soothing liver-QI injection for curing is liver-protecting medicine, mainly by Herba Artemisiae Scopariae extract, Fructus Gardeniae extract, baicalin, plate
Blue root extract and Ganoderma extract are made.There is heat-clearing and toxic substances removing, dampness removing jaundice eliminating, QI invigorating righting, protect the liver and protect
Effect of liver.For jaundice due to damp-heat, disease meeting mesh is the most yellow, costa sternales distension, nausea and vomiting, yellowish or reddish urine,
Weak, poor appetite, loose stool；Acute chronic viral hepatitis is shown in foregoing conditions person.
Inventor's raw material to making used by soothing liver-QI injection for curing, Radix Isatidis extract and Herba Artemisiae Scopariae extract and
Extraction process has carried out substantial amounts of research, and inventor finds in research process, uses existing Radix Isatidis to extract
In the soothing liver-QI injection for curing that thing and Herba Artemisiae Scopariae extract are made, in Radix Isatidis, indigo being difficult to is measured.And in Radix Isatidis
The indigo principle active component for Radix Isatidis, but Radix Isatidis extract and the Herba Artemisiae Scopariae prepared by prior art carry
Taking in the easypro treating hepatopathy that thing makes, indigo content is the highest, have impact on the curative effect of medicine.
Summary of the invention
The invention provides the preparation method of the peaceful ejection preparation of a kind of soothing liver-QI, wherein Radix Isatidis take thing and
The extracting method of Herba Artemisiae Scopariae extract, is improved its concrete technology and parameter, and make in Radix Isatidis is effective
Composition is indigo easily to be measured, and the indigo content measured is high.Can effectively improve the peaceful ejection preparation of soothing liver-QI
Drug effect.
To achieve these goals, the present invention provides following technical scheme: the system of the peaceful ejection preparation of a kind of soothing liver-QI
Preparation Method, described easypro treating hepatopathy calculates by weight, is mainly extracted by Herba Artemisiae Scopariae extract 1-8 part, Fructus Gardeniae
Thing 1-5 part, baicalin 15-25 part, Radix Isatidis extract 1-10 part, Ganoderma extract 1-7 part are by following
Method is made: adds water for injection to baicalin so that it is suspended, then adds 10% sodium hydroxide solution and make dissolving；
It is separately added into water for injection in Herba Artemisiae Scopariae extract, Fructus Gardeniae extract, Radix Isatidis extract and Ganoderma extract,
Make dissolving, mixing, add activated carbon, stir evenly, boil, filter, with 10% sodium hydroxide solution regulation pH value
To 7.5～8.0, inject water to ormal weight, filter, embedding, sterilizing, to obtain final product；Described Herba Artemisiae Scopariae is extracted
The extracting method of thing is: take Herba Artemisiae Scopariae, after soaking with soak, extracts with alcohol heating reflux, filters, medicine
Slag is standby, extracting solution decompression recycling ethanol, and concentrated solution adds water, and upper macroporous adsorbent resin adds water elution, then
With ethanol elution, collect eluent, reclaim ethanol, obtain alcohol extract, standby；Medicinal residues add water extraction,
Filter, merging filtrate, after being condensed into concentrated solution, merge with above-mentioned alcohol extract, be condensed into extractum, to obtain final product；
The extracting method of described Radix Isatidis extract is: take Radix Isatidis, after soaking with soak, after soaking,
Decocting, filter, filtering residue adds water extraction, filters, merging filtrate, after being condensed into concentrated solution, obtains water and carry
Liquid, standby；Filtering residue adds ethanol solution, reflux, extract, filters, merging filtrate, reclaims ethanol,
Alcohol extract, alcohol extract merges with above-mentioned Aqueous extracts, is condensed into extractum, dry, pulverize, to obtain final product.
In the preparation method of the peaceful ejection preparation of aforesaid soothing liver-QI, described easypro treating hepatopathy calculates by weight, main
Will be by Herba Artemisiae Scopariae extract 4 parts, Fructus Gardeniae extract 3 parts, baicalin 22 parts, Radix Isatidis extract 5 parts, spirit
Sesame extract 3.5 parts is made as follows: adds water for injection to baicalin so that it is suspended, then adds 10%
Sodium hydroxide solution makes dissolving；Extract to Herba Artemisiae Scopariae extract, Fructus Gardeniae extract, Radix Isatidis extract and Ganoderma
Thing is dissolved in water for injection respectively, mixing, adds 0.2% activated carbon, stir evenly, boil 15 minutes, filter,
With 10% sodium hydroxide solution regulation pH value to 7.5～8.0, inject water to ormal weight, filter, embedding,
Sterilizing, to obtain final product.
In the preparation method of the peaceful ejection preparation of aforesaid soothing liver-QI, the extracting method of described Herba Artemisiae Scopariae extract is: take
Herba Artemisiae Scopariae, the soak adding 5-7 times amount soaks 8-12h, filters, then add 8-12 times amount 70-85% ethanol and add
Circumfluence distillation 1-5 time, each 0.5-3 hour, filters, and medicinal residues are standby, extracting solution decompression recycling ethanol,
It is 1-3:3-1 that concentrated solution adds water to the ratio of volume every milliliter and medical material amount every gram, and upper macroporous adsorbent resin adds
4-10 times of column volume of water elution, then with 4-10 times of column volume eluting of 30-70% ethanol, collect eluent, return
Receive ethanol, obtain alcohol extract, standby；Medicinal residues add 10 times amount water extraction 2-3 time, each 1-3 hour,
Filter, merging filtrate, after being condensed into concentrated solution, merge with above-mentioned alcohol extract, phase at being concentrated into 50-60 DEG C
It is the extractum of 1.1-1.2 to density, to obtain final product.
In the preparation method of the peaceful ejection preparation of aforesaid soothing liver-QI, the extracting method of described Herba Artemisiae Scopariae extract is: take
Herba Artemisiae Scopariae, the soak adding 5-7 times amount soaks 8-12h, filters, then add 10 times amount 80% ethanol and heat back
Stream extracts 3 times, each 2 hours, filters, and medicinal residues are standby, extracting solution decompression recycling ethanol, and concentrated solution adds
Water is 1.5:1.5 to the ratio of volume every milliliter with medical material amount every gram, and upper macroporous adsorbent resin adds water elution 7
Times column volume, then with 7 times of column volume eluting of 50% ethanol, collect eluent, reclaim ethanol, obtain alcohol extract,
Standby；Medicinal residues add 10 times amount water extraction 3 times, each 2 hours, filter, merging filtrate, are condensed into
After concentrated solution, merging with above-mentioned alcohol extract, at being concentrated into 50-60 DEG C, relative density is the extractum of 1.1-1.2,
Obtain.
In the preparation method of the peaceful ejection preparation of aforesaid soothing liver-QI, the extracting method of described Radix Isatidis extract is:
Taking Radix Isatidis, the soak adding 5-7 times amount soaks 8-12h, filters, adds the water soaking of 8-12 times amount
After 10-30min, decocting 2-3h, filter, filtering residue adds 6-10 times amount water extraction 1-3 time, each 1-3h,
Filter, merging filtrate, after being condensed into concentrated solution, obtain Aqueous extracts, standby；Filtering residue adds 6-10 times amount 55-65%
Ethanol solution, reflux, extract, 2-3 time, each 0.5-3h, filter, merging filtrate, reclaim ethanol,
Alcohol extract, alcohol extract merges with above-mentioned Aqueous extracts, and when being concentrated into 50-60 DEG C, relative density is 1.10-1.20
Extractum, dry, pulverize, to obtain final product.
In the preparation method of the peaceful ejection preparation of aforesaid soothing liver-QI, the extracting method of described Radix Isatidis extract is:
Taking Radix Isatidis, the soak adding 5-7 times amount soaks 8-12h, filters, adds the water soaking of 10 times amount
After 20min, decocting 2.5h, filter, filtering residue adds 8 times amount water extraction 2 times, each 2h, filters,
Merging filtrate, after being condensed into concentrated solution, obtains Aqueous extracts, standby；The ethanol that filtering residue adds 8 times amount 60% is molten
Liquid, reflux, extract, 3 times, each 2h, filters, merging filtrate, reclaims ethanol, obtain alcohol extract, alcohol extraction
Liquid merges with above-mentioned Aqueous extracts, and when being concentrated into 50-60 DEG C, relative density is the extractum of 1.10-1.20, is dried,
Pulverize, to obtain final product.
In the preparation method of the peaceful ejection preparation of aforesaid soothing liver-QI, soak described in described soak is prepared:
Take the white vinegar of 2-8 parts by volume, add in the boiling water of 90-110 parts by volume, add the green tea of 5-15 weight portion,
Stir a little, stand 20-40 minute, filter, to obtain final product.
In the preparation method of the peaceful ejection preparation of aforesaid soothing liver-QI, described soak is prepared: take 5 parts by volume
White vinegar, add in the boiling water of 100 parts by volume, add the green tea of 10 weight portions, stir a little, stand
30 minutes, filter, to obtain final product.
The peaceful ejection preparation of soothing liver-QI of the present invention is mainly by Herba Artemisiae Scopariae extract, Fructus Gardeniae extract, baicalin and Radix Isatidis
Extract is made.Wherein, Herba Artemisiae Scopariae, ArtemisiacapillarisThunb, meridian distribution of property and flavor: bitter, pungent, it is slightly cold.
Return spleen, stomach, liver, gallbladder meridian.There is Herba Artemisiae Scopariae clearing away heat-damp and promoting diuresis, effect of jaundice eliminating.Can be used for treating jaundice, little
The most unfavorable, eczema pruritus, infectious jaundice type liver.Fructus Gardeniae (formal name used at school: Gardeniajasminoides Ellis),
Belong to the l that Ministry of Public Health promulgates and criticize medicine-food two-purpose resource, there is hepatoprotective, function of gallbladder promoting, blood pressure lowering, calmness, stop blooding, disappear
Swollen grade acts on.It is usually used in treating the diseases such as icterohepatitis, bruise, hypertension, diabetes at tcm clinical practice.
Baicalin (Baicalin) is to extract a kind of flavone compound separated from scutellariae,radix, has significantly
Biological activity, has antibacterial, diuresis, antiinflammatory, resistance state and a spasmolysis, and has stronger anticancer
The physiological potencies such as reaction.Critical role has been occupied at clinical medicine.Baicalin can also absorb ultraviolet, removes
Oxygen-derived free radicals, can suppress again melanic generation, therefore can be not only used for medicine it can also be used to cosmetics, be
A kind of good functional cosmetics raw material.There is removing the relative excess fire, dehumidifying heat, hemostasis, antiabortive effect.
Can be used for high fever excessive thirst, cough due to lung-heat, damp-heat dysentery, jaundice, pyretic stranguria, tell, be defeated in battle, collapse, leak, conjunctival congestion
Swell and ache, frequent fetal movement, carbuncle furuncle.Radix Isatidis, Isatis tinctoria, doing for cruciferae isatis
Dry, cold in nature, taste is the most micro-sweet rear pained, has effect of heat-clearing and toxic substances removing, preventing cold, sore-throat relieving.Mainly
For treating the disease such as maculae caused by violent heat pathogen, purple dark, the scarlet fever of crimson tongue.
Applicant has carried out long-term substantial amounts of research to the making of soothing liver-QI injection for curing, it has been found that adopt
It is raw material with existing Radix Isatidis extract and Herba Artemisiae Scopariae extract, in the peaceful ejection preparation of soothing liver-QI made, Radix Isatidis
Indigo being difficult to of the effective ingredient of extract is measured.Inventor finds in technical study, uses of the present invention
The Radix Isatidis extract that method is made is as raw material, in the peaceful ejection preparation of soothing liver-QI made, having in Radix Isatidis
The effect indigo content of composition adds a lot unexpectedly, and therefore to promote effective ingredient in Radix Isatidis indigo for this technique
Dissolution rate.Indigo (indigo) is the main active ingredient in Radix Isatidis.This technique visible promotes Baphicacanthus cusia
Dissolution indigo in root, can effectively improve the drug effect of the peaceful ejection preparation of soothing liver-QI.
Compared with prior art, in the peaceful ejection preparation of soothing liver-QI that the method for the invention makes, Radix Isatidis extracts
Effective ingredient in thing is indigo easily to be measured, and it is high to measure indigo content.Can effectively improve soothing liver-QI rather to note
Penetrate the drug effect of preparation.
The present invention has carried out substantial amounts of experimentation, is below the result of experimentation of the present invention:
The indigo content of experimental example 1 is investigated
1 raw material
1.1 injections of the present invention, make as described in Example 1.
1.2 existing injections: make by following technique:
Prescription: Herba Artemisiae Scopariae extract 4g, Fructus Gardeniae extract 3g, baicalin 22g, Radix Isatidis extract 5g, spirit
Sesame extract 3.5g and adjuvant.
Technique: add the water for injection of 1.5 times amount to baicalin so that it is suspended, then it is molten to add 10% sodium hydroxide
Liquid makes dissolving；Difference 1.5 in Herba Artemisiae Scopariae extract, Fructus Gardeniae extract, Radix Isatidis extract and Ganoderma extract
Being dissolved in water for injection of times amount, mixing, add activated carbon, stir evenly, boil, filter, use 10% hydroxide
Sodium solution regulation pH value, to 7.5～8.0, injects water to ormal weight, filters, embedding, sterilizing, to obtain final product.
Wherein, Radix Isatidis extract and Herba Artemisiae Scopariae extract are commercially available.
1.3SPD-LC10A type high performance liquid chromatograph (Japan's Shimadzu)；BP210S electronic balance (moral
Sartorius company of state).Indigo reference substance (Nat'l Pharmaceutical & Biological Products Control Institute)；Ethanol is medicinal rule
Lattice, other reagent are analytical pure and chromatographically pure.
2 experimental techniques and result
2.1 chromatographic condition
Dikma DiamonsilTMC18Chromatographic column (5um, 200mm*4.6mm), flowing is methanol-0.1 mutually
Mol/L ammonium acetate-acetic acid (70:30:1), flow velocity 1ml/min, detects wavelength 280nm, column temperature 40 DEG C.
2.2 the preparation of reference substance solution
Accurate weighing is dried the indigo reference substance about 2mg to constant weight respectively, is placed in 50ml measuring bottle, adds chloroform
Dissolve and constant volume, to obtain final product.
The preparation of 2.3 need testing solutions
The preparation of need testing solution 1: precision measures injection 2ml of the present invention, puts in 25ml brown measuring bottle,
Add methanol to scale, shake up, to obtain final product.
The preparation of need testing solution 2: precision measures existing injection 2ml, puts in 25ml brown measuring bottle, adds
Methanol, to scale, shakes up, and to obtain final product.
The investigation of 2.4 linear relationships
Under above-mentioned chromatographic condition, accurate draw indigo reference substance solution 1,2.5,5,10,15,20ul
Injecting high performance liquid chromatograph, measure peak area, with indigo peak area as vertical coordinate (Y), indigo content is
Abscissa (X), calculates regression equation: Y=6.836*106X-1.328*104(r=0.9995, n=5).
Result shows that indigo sample size has good linear relationship in the range of 0.0152-0.304ug.
2.5 precision test
Take reference substance solution, repeat sample introduction 6 times, each 5ul, the RSD=1.58% of indigo peak area, table
Bright instrument precision is good.
2.6 stability test
Essence takes need testing solution 1, presses sample determination method in 10h, measures once every 2h.Indigo content
RSD=0.34%, show that need testing solution is stable in 10h.
Essence takes need testing solution 2, presses sample determination method in 10h, measures once every 2h.Indigo content
RSD=0.36%, show that need testing solution is stable in 10h.
2.7 replica test
Take with batch of injection of the present invention 6 parts, every part of about 2ml, accurately weighed, by need testing solution 1 side of preparation
Prepared by method, measure in accordance with the law, result shows, RSD is 1.05%, and the method repeatability is good.
Take with batch of existing injection 6 parts, every part of about 2ml, accurately weighed, by need testing solution 2 preparation method
Preparation, measures in accordance with the law, and result shows, RSD is 1.07%, and the method repeatability is good.
2.8 sample-adding recovery tests
Take the injection of the present invention of known content and existing injection 2ml respectively, accurately weighed, parallel 9 parts, point
The inaccurate reference substance solution that adds is appropriate, prepares need testing solution 1 by test sample preparation method, according to sample determination
Method measures, and calculates the response rate.End product is that average recovery rate is respectively 97.87% and 97.73%,
RSD=0.62% and RSD=0.85%.Therefore this method reliability is good.
The mensuration of 2.9 samples
Measure injection of the present invention and existing injection 2ml respectively, accurately weighed, by need testing solution 1 with for examination
Prepared by product solution 2 preparation method, parallel measure 6 parts, by above-mentioned chromatographic condition, sample size 10ul, surveys
Fixed, external standard method calculates content, the results are shown in Table 1..
The assay result that table 1 is indigo
As known from Table 1, average content indigo in injection of the present invention is 0.688ug/g, in existing injection
Indigo average content is 0.498ug/g.
Conclusion: compared with existing injection, the content that in injection of the present invention, effective ingredient of isatis root is indigo is more
High.In like manner, according to the preparation of other dosage forms that extracting method of the present invention is made, the effective ingredient of Radix Isatidis contains
Amount also should increase.
Experimental example 2. effect experiment
1 materials and methods
1.1 trial drug
Injection the most of the present invention, with embodiment 1.
The most existing injection: with experimental example 1.
1.2 animal Kunming mouses, male, body weight 18-22g, Sichuan Academy of Medical Sciences's laboratory animal is ground
Study carefully and provided.
1.3 reagent A LT (GTT), AST (GOT) test kit, Mei Sheng Industrial Co., Ltd. produces.Carbon tetrachloride
(analytical pure), Tianjin Bo Di Chemical Co., Ltd. produces.Thioacetamide, Beijing chemical reagent three factory is raw
Produce,.56 degree of Red Star strong, colourless liquor distilled from sorghum, Hongxing Co., Ltd. Beijing produces.
1.4 instrument TMS-1024i type automatic clinical chemistry analyzers, Tokyo trade Co., Ltd..
Multiskan MK3 type microplate reader, Thermo Labsystems company.
2 experimental techniques
40 mices are randomly divided into 4 groups, i.e. Normal group, model group, of the present invention group and existing preparation group,
Often group 10, each group tail vein injection is administered.Injecting injection of the present invention for of the present invention group, concentration is
0.1ml/10g, is configured to the medicinal liquid of 40%, 20%, 10% before use with Dextrose and Sodium Chloride Inj. dilution,
Injection.Existing preparation group injects existing injection, and dosage is 0.1ml/10g, uses glucose sodium chloride before use
Injection dilution is configured to the medicinal liquid of 40%, 20%, 10%, injection.Normal group and model group injection
Normal saline, is given daily 1 time, continuous 8 days.From being administered the 4th day, after every day is administered 0.5h, little
56 degree of Red Stars strong, colourless liquor distilled from sorghum Chinese liquor 8mL/kg (normal group is to equal volume distilled water) of Mus gavage, 2 times/day,
Two minor tick 3-4h, mice is administered and fills all fasting before wine.Continuous 5 days, 1h after last gavage Chinese liquor,
Extract eyeball of mouse and take blood, separate serum, measure serum alt and AST level, anatomical isolation liver,
Weighing, calculate liver index, separately take same area hepatic tissue, 10% formaldehyde is fixed, section, after HE dyeing
Observation liver pathomorphology changes.The results are shown in Table 2, table 3.
Table 2 is on acute alcohol liver injury model mouse liver index and Serum ALT, the impact of AST level
Compare with Normal group,#P < 0.01,##P<0.01；
Compare with model group,*P < 0.05,**P<0.01；
Compare with existing ejection preparation group,+P<0.05；
The table 3 impact on acute alcohol liver injury model mouse liver Histopathologic changes
Note: pathological changes classification standard: " one ", normally, without pathological changes such as hepatocyte puffing, balloon sample changes；
"+", lobules of liver is less than 1/3 region slight hepatic cell swelling, endochylema puffing.But hepatic cords is basic
Clear, sinus hepaticus is the most visible." ++ ", moderate endochylema puffing, between slight hepatic cell swelling and balloon sample
Between, the slightly aobvious disorder of liver rope, sinus hepaticus starts to occur narrow.
As seen from table, the peaceful ejection preparation of soothing liver-QI of the present invention and the peaceful ejection preparation of existing soothing liver-QI all have reduction Mouse Blood
The effect of ALT and AST level, and soothing liver-QI of the present invention peaceful ejection preparation effect clearly is better than existing soothing liver-QI and rather injects
Preparation.Histopathologic examination shows, model group mouse liver cell occur endochylema puffing in various degree,
Cellular edema, balloon sample become, lytic necrosis, and the pathological changes such as liver rope is disorderly, sinus hepaticus is narrow, soothing liver-QI is peaceful
Injection successive administration 8 days, the peaceful ejection preparation of soothing liver-QI of the present invention and the peaceful ejection preparation of existing soothing liver-QI all present not
Improvement result with degree.Wherein, soothing liver-QI of the present invention peaceful ejection preparation effect is better than existing soothing liver-QI and rather injects system
Agent.
Detailed description of the invention:
Embodiment 1:
Formula: Herba Artemisiae Scopariae extract 4g, Fructus Gardeniae extract 3g, baicalin 22g, Radix Isatidis extract 5g and spirit
Sesame extract 3.5g.
The preparation method of the peaceful injection of soothing liver-QI: add water for injection to baicalin so that it is suspended, then add 10%
Sodium hydroxide solution makes dissolving；Extract to Herba Artemisiae Scopariae extract, Fructus Gardeniae extract, Radix Isatidis extract and Ganoderma
Thing is dissolved in water for injection respectively, mixing, adds 0.2% activated carbon, stir evenly, boil 15 minutes, filter,
With 10% sodium hydroxide solution regulation pH value to 7.5～8.0, inject water to 1000ml, filter, embedding,
Sterilizing, obtains injection.
The extraction process of Herba Artemisiae Scopariae extract: take Herba Artemisiae Scopariae, the soak adding 6 times amount soaks 8-12h, filters,
Adding 10 times amount 80% alcohol heating reflux again to extract 3 times, each 2 hours, filter, medicinal residues are standby, extract
Liquid decompression recycling ethanol, it is 1.5:1.5 that concentrated solution adds water to the ratio of volume every milliliter and medical material amount every gram, on
Macroporous adsorbent resin, adds 7 times of column volumes of water elution, then with 7 times of column volume eluting of 50% ethanol, collects eluting
Liquid, reclaims ethanol, obtains alcohol extract, standby；Medicinal residues add 10 times amount water extraction 3 times, each 2 little
Time, filter, merging filtrate, after being condensed into concentrated solution, merge with above-mentioned alcohol extract, be concentrated into 50-60 DEG C
Lower relative density is the extractum of 1.1-1.2, to obtain final product.
The extracting method of Radix Isatidis extract is: take Radix Isatidis, and the soak adding 6 times amount soaks 8-12h,
Filtering, after adding the water soaking 20min of 10 times amount, decoct 2.5h, filter, filtering residue adds 8 times amount
Water extraction 2 times, each 2h, filter, merging filtrate, after being condensed into concentrated solution, obtain Aqueous extracts, standby；
Filtering residue adds the ethanol solution of 8 times amount 60%, reflux, extract, 3 times, each 2h, filters, merging filtrate,
Reclaiming ethanol, obtain alcohol extract, alcohol extract merges with above-mentioned Aqueous extracts, relative density when being concentrated into 50-60 DEG C
For the extractum of 1.10-1.20, dry, pulverize, to obtain final product.
Described soak is prepared: take the white vinegar of 50ml, adds in the boiling water of 1000ml, adds 100g
Green tea, stir a little, stand 30 minutes, filter, to obtain final product.
Usage and dosage: intravenous drip, one time 10～20ml, with 10% glucose injection 250～500ml
Dilution posterior vein instils, 1 time on the one；Intramuscular injection can be used instead after remission, one day 2～4ml, one day
1 time.
Points for attention: 1, anemia of pregnant woman and allergic constitution person are cautious use of；
2, close observation medicinal liquid character before injection, forbids to make when having muddiness, precipitation, floccule
With.
Specification: every dress 2ml, 10ml or 20ml.
Embodiment 2:
Formula: Herba Artemisiae Scopariae extract 4g, Fructus Gardeniae extract 3g, baicalin 22g, Radix Isatidis extract 5g and spirit
Sesame extract 3.5g.
The preparation method of the peaceful injection of soothing liver-QI: add water for injection to baicalin so that it is suspended, then add 10%
Sodium hydroxide solution makes dissolving；Extract to Herba Artemisiae Scopariae extract, Fructus Gardeniae extract, Radix Isatidis extract and Ganoderma
Thing is dissolved in water for injection respectively, mixing, adds 0.2% activated carbon, stir evenly, boil 15 minutes, filter,
With 10% sodium hydroxide solution regulation pH value to 7.5～8.0, inject water to 1000ml, filter, embedding,
Sterilizing, obtains injection.
The extraction process of Herba Artemisiae Scopariae extract: take Herba Artemisiae Scopariae, the soak adding 7 times amount soaks 12h, filters, then
Adding 12 times amount 85% alcohol heating reflux to extract 5 times, each 3 hours, filter, medicinal residues are standby, extracting solution
Decompression recycling ethanol, it is 3:1 that concentrated solution adds water to the ratio of volume every milliliter and medical material amount every gram, and upper macropore is inhaled
Attached resin, adds 4-10 times of column volume of water elution, then with 10 times of column volume eluting of 70% ethanol, collects eluent,
Reclaim ethanol, obtain alcohol extract, standby；Medicinal residues add 10 times amount water extraction 3 times, each 3 hours,
Filter, merging filtrate, after being condensed into concentrated solution, merge with above-mentioned alcohol extract, phase at being concentrated into 50-60 DEG C
It is the extractum of 1.1-1.2 to density, to obtain final product.
The extracting method of Radix Isatidis extract is: take Radix Isatidis, and the soak adding 7 times amount soaks 12h, mistake
Filter, after adding the water soaking 30min of 12 times amount, decocts 3h, filters, and filtering residue adds 10 times amount water
Extract 3 times, each 3h, filter, merging filtrate, after being condensed into concentrated solution, obtain Aqueous extracts, standby；Filter
Slag adds the ethanol solution of 10 times amount 65%, reflux, extract, 3 times, each 3h, filters, merging filtrate,
Reclaiming ethanol, obtain alcohol extract, alcohol extract merges with above-mentioned Aqueous extracts, relative density when being concentrated into 50-60 DEG C
For the extractum of 1.10-1.20, dry, pulverize, to obtain final product.
Described soak is prepared: take the white vinegar of 80ml, adds in the boiling water of 1100ml, adds 150g
Green tea, stir a little, stand 40 minutes, filter, to obtain final product.
Usage and dosage and specification are with embodiment 1.
Embodiment 3:
Formula: Herba Artemisiae Scopariae extract 4g, Fructus Gardeniae extract 3g, baicalin 22g, Radix Isatidis extract 5g and spirit
Sesame extract 3.5g.
The preparation method of the peaceful injection of soothing liver-QI: add water for injection to baicalin so that it is suspended, then add 10%
Sodium hydroxide solution makes dissolving；Extract to Herba Artemisiae Scopariae extract, Fructus Gardeniae extract, Radix Isatidis extract and Ganoderma
Thing is dissolved in water for injection respectively, mixing, adds 0.2% activated carbon, stir evenly, boil 15 minutes, filter,
With 10% sodium hydroxide solution regulation pH value to 7.5～8.0, inject water to 1000ml, filter, embedding,
Sterilizing, obtains injection.
The extraction process of Herba Artemisiae Scopariae extract: take Herba Artemisiae Scopariae, the soak adding 5 times amount soaks 8h, filters, then
Adding 8 times amount 70% alcohol heating reflux to extract 3 hours, filter, medicinal residues are standby, extracting solution recovered under reduced pressure second
Alcohol, it is 1:3 that concentrated solution adds water to the ratio of volume every milliliter and medical material amount every gram, and upper macroporous adsorbent resin adds
4 times of column volumes of water elution, then with 4 times of column volume eluting of 30% ethanol, collect eluent, reclaim ethanol,
Alcohol extract, standby；Medicinal residues add 10 times amount water extraction 2 times, each 1 hour, filter, and merge filter
Liquid, after being condensed into concentrated solution, merges with above-mentioned alcohol extract, and at being concentrated into 50-60 DEG C, relative density is
1.1-1.2 extractum, to obtain final product.
The extracting method of Radix Isatidis extract is: take Radix Isatidis, and the soak adding 5 times amount soaks 8h, mistake
After filter, then the water soaking 10min of addition 8 times amount, decocting 2h, filter, filtering residue adds 6 times amount water
Extract 1h, filter, filtrate, after being condensed into concentrated solution, obtain Aqueous extracts, standby；Filtering residue adds 6 times amount
The ethanol solution of 55%, reflux, extract, 2 times, each 0.5h, filters, merging filtrate, reclaims ethanol, obtain alcohol
Extracting solution, alcohol extract merges with above-mentioned Aqueous extracts, and when being concentrated into 50-60 DEG C, relative density is 1.10-1.20's
Extractum, dry, pulverize, and to obtain final product.
Described soak is prepared: take the white vinegar of 20ml, adds in the boiling water of 900ml, adds 50g
Green tea, stir a little, stand 20 minutes, filter, to obtain final product.
Usage and dosage and specification are with embodiment 1.
Claims (8)
1. the preparation method of the peaceful ejection preparation of soothing liver-QI, it is characterised in that: described easypro treating hepatopathy is by weight
Calculate, mainly by Herba Artemisiae Scopariae extract 1-8 part, Fructus Gardeniae extract 1-5 part, baicalin 15-25 part, Radix Isatidis
Extract 1-10 part, Ganoderma extract 1-7 part are made as follows: add water for injection to baicalin,
Make it suspended, then add 10% sodium hydroxide solution and make dissolving；To Herba Artemisiae Scopariae extract, Fructus Gardeniae extract, Radix Isatidis
Extract and Ganoderma extract are separately added into water for injection, make dissolving, mixing, add activated carbon, stir evenly,
Boil, filter, with 10% sodium hydroxide solution regulation pH value to 7.5～8.0, inject water to ormal weight,
Filter, embedding, sterilizing, to obtain final product；The extracting method of described Herba Artemisiae Scopariae extract is: takes Herba Artemisiae Scopariae, uses soak
After immersion, extracting with alcohol heating reflux, filter, medicinal residues are standby, extracting solution decompression recycling ethanol, concentrate
Liquid adds water, upper macroporous adsorbent resin, adds water elution, then with ethanol elution, collects eluent, reclaims ethanol,
Obtain alcohol extract, standby；Medicinal residues add water extraction, filter, merging filtrate, after being condensed into concentrated solution,
Merge with above-mentioned alcohol extract, be condensed into extractum, to obtain final product；The extracting method of described Radix Isatidis extract is:
Taking Radix Isatidis, after soaking with soak, after soaking, decoct, filter, filtering residue adds water extraction,
Filter, merging filtrate, after being condensed into concentrated solution, obtain Aqueous extracts, standby；Filtering residue adds ethanol solution,
Reflux, extract, filters, merging filtrate, reclaims ethanol, obtains alcohol extract, and alcohol extract closes with above-mentioned Aqueous extracts
And, it is condensed into extractum, dry, pulverize, to obtain final product.
2. the preparation method of the peaceful ejection preparation of soothing liver-QI as claimed in claim 1, it is characterised in that: described soothing liver-QI is peaceful
Preparation calculates by weight, mainly by Herba Artemisiae Scopariae extract 4 parts, Fructus Gardeniae extract 3 parts, baicalin 22 parts,
Radix Isatidis extract 5 parts, Ganoderma extract 3.5 parts are made as follows: add injection to baicalin
Water so that it is suspended, then add 10% sodium hydroxide solution and make dissolving；To Herba Artemisiae Scopariae extract, Fructus Gardeniae extract, plate
Blue root extract and Ganoderma extract are dissolved in water for injection respectively, mixing, add 0.2% activated carbon, stir evenly,
Boil 15 minutes, filter, with 10% sodium hydroxide solution regulation pH value to 7.5～8.0, inject and use water
To ormal weight, filter, embedding, sterilizing, to obtain final product.
3. the preparation method of the peaceful ejection preparation of soothing liver-QI as claimed in claim 1, it is characterised in that: described Herba Artemisiae Scopariae carries
The extracting method taking thing is: take Herba Artemisiae Scopariae, and the soak adding 5-7 times amount soaks 8-12h, filters, then adds 8-12
Times amount 70-85% alcohol heating reflux extracts 1-5 time, each 0.5-3 hour, filters, and medicinal residues are standby, carry
Taking liquid decompression recycling ethanol, it is 1-3:3-1 that concentrated solution adds water to the ratio of volume every milliliter and medical material amount every gram,
Upper macroporous adsorbent resin, adds 4-10 times of column volume of water elution, then washes with 4-10 times of column volume of 30-70% ethanol
De-, collect eluent, reclaim ethanol, obtain alcohol extract, standby；Medicinal residues add 10 times amount water extraction 2-3
Secondary, each 1-3 hour, filter, merging filtrate, after being condensed into concentrated solution, merge with above-mentioned alcohol extract,
At being concentrated into 50-60 DEG C, relative density is the extractum of 1.1-1.2, to obtain final product.
4. the preparation method of the peaceful ejection preparation of soothing liver-QI as claimed in claim 3, it is characterised in that: described Herba Artemisiae Scopariae carries
The extracting method taking thing is: take Herba Artemisiae Scopariae, and the soak adding 6 times amount soaks 10h, filters, then adds 10 times
Measuring 80% alcohol heating reflux to extract 3 times, each 2 hours, filter, medicinal residues are standby, and extracting solution reduces pressure back
Receiving ethanol, it is 1.5:1.5 that concentrated solution adds water to the ratio of volume every milliliter and medical material amount every gram, upper macroporous absorption
Resin, adds 7 times of column volumes of water elution, then with 7 times of column volume eluting of 50% ethanol, collects eluent, reclaim
Ethanol, obtains alcohol extract, standby；Medicinal residues add 10 times amount water extraction 3 times, each 2 hours, filter,
Merging filtrate, after being condensed into concentrated solution, merges with above-mentioned alcohol extract, relative density at being concentrated into 50-60 DEG C
For the extractum of 1.1-1.2, to obtain final product.
5. the preparation method of the peaceful ejection preparation of soothing liver-QI as claimed in claim 1, it is characterised in that: described Radix Isatidis
The extracting method of extract is: take Radix Isatidis, and the soak adding 5-7 times amount soaks 8-12h, filters, then
After adding the water soaking 10-30min of 8-12 times amount, decocting 2-3h, filter, filtering residue adds 6-10 times amount
Water extraction 1-3 time, each 1-3h, filter, merging filtrate, after being condensed into concentrated solution, obtain Aqueous extracts, standby
With；Filtering residue adds the ethanol solution of 6-10 times amount 55-65%, reflux, extract, 2-3 time, each 0.5-3h,
Filtering, merging filtrate, reclaim ethanol, obtain alcohol extract, alcohol extract merges with above-mentioned Aqueous extracts, is concentrated into
When 50-60 DEG C, relative density is the extractum of 1.10-1.20, dry, pulverize, and to obtain final product.
6. the preparation method of the peaceful ejection preparation of soothing liver-QI as claimed in claim 5, it is characterised in that: described Radix Isatidis
The extracting method of extract is: take Radix Isatidis, and the soak adding 6 times amount soaks 10h, filters, adds
After the water soaking 20min of 10 times amount, decocting 2.5h, filter, filtering residue adds 8 times amount water extraction 2 times,
2h every time, filters, merging filtrate, after being condensed into concentrated solution, obtains Aqueous extracts, standby；Filtering residue adds 8
The ethanol solution of times amount 60%, reflux, extract, 3 times, each 2h, filter, merging filtrate, reclaim ethanol,
Obtaining alcohol extract, alcohol extract merges with above-mentioned Aqueous extracts, and when being concentrated into 50-60 DEG C, relative density is 1.10-1.20
Extractum, dry, pulverize, to obtain final product.
7. the preparation method of the peaceful ejection preparation of soothing liver-QI as described in claim 1,3,4,5 or 6, it is characterised in that:
Soak described in described soak is prepared: take the white vinegar of 2-8 parts by volume, adds 90-110 parts by volume
In boiling water, add the green tea of 5-15 weight portion, stir a little, stand 20-40 minute, filter, to obtain final product.
8. the preparation method of the peaceful ejection preparation of soothing liver-QI as claimed in claim 7, it is characterised in that: described soak this
Prepared by sample: take the white vinegar of 5 parts by volume, adds in the boiling water of 100 parts by volume, adds the green of 10 weight portions
Tea, stirs a little, stands 30 minutes, filters, to obtain final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610338254.3A CN105920145A (en) | 2016-05-20 | 2016-05-20 | Preparation method of liver-smoothing injection preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610338254.3A CN105920145A (en) | 2016-05-20 | 2016-05-20 | Preparation method of liver-smoothing injection preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105920145A true CN105920145A (en) | 2016-09-07 |
Family
ID=56840938
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610338254.3A Withdrawn CN105920145A (en) | 2016-05-20 | 2016-05-20 | Preparation method of liver-smoothing injection preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105920145A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115112801A (en) * | 2022-07-04 | 2022-09-27 | 贵州瑞和制药有限公司 | Method for simultaneously determining content of 10 components in Shuganning injection |
-
2016
- 2016-05-20 CN CN201610338254.3A patent/CN105920145A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115112801A (en) * | 2022-07-04 | 2022-09-27 | 贵州瑞和制药有限公司 | Method for simultaneously determining content of 10 components in Shuganning injection |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101396544B (en) | Traditional Chinese medicine composition capable of ventilating the lung and relieving asthma and preparation method thereof | |
| CN100457139C (en) | Method for preparing a Shuanhuanglian injection and the component detecting method | |
| CN105963390A (en) | Preparation method of Shuganning preparation | |
| CN100418512C (en) | 'Shengmai' infusion and its preparation process | |
| CN101843667B (en) | Shuanghuanglian medicinal composition and preparation method thereof | |
| CN102210737A (en) | Sweet potato leaf extract and preparation method and use thereof | |
| CN100544758C (en) | A kind of compound Taizi ginsen preparation and production method thereof | |
| CN102539599B (en) | Method for detecting liver-enhancing medicine | |
| CN105412294B (en) | A kind of production method of easypro treating hepatopathy | |
| CN101972465B (en) | Medicinal preparation for treating primary dysmenorrhea and preparation method thereof | |
| CN105412295B (en) | A kind of manufacture craft of easypro treating hepatopathy | |
| CN105920145A (en) | Preparation method of liver-smoothing injection preparation | |
| CN103550237B (en) | Composition of active ingredients of rubia cordifolia as well as application of composition in medicines | |
| CN106620188B (en) | A kind of preparation method of clearing heat and detoxicating particle | |
| CN103610798B (en) | Active site of a kind of Herba Hedyotidis Diffusae and preparation method thereof, application | |
| CN105902651A (en) | Production method of shuganning injection preparation | |
| CN105434342A (en) | Preparation method for Shuganning injection preparation | |
| CN102362993B (en) | Chinese medicinal preparation with effects of protecting intestines and removing toxic materials, and preparation method thereof | |
| CN103156997A (en) | Composition of effective parts of traditional Chinese medicines for treating chronic hepatopathy, preparation method and application thereof | |
| CN102631386B (en) | Bupleurum antipyretic and analgesic preparation and technology for preparing same | |
| CN105878469A (en) | Preparation technology of Shuganning injection preparation | |
| CN105902488A (en) | Process for preparing Shuganning preparation | |
| CN101537056A (en) | Method for preparing Chinese medicinal preparation for treating external respiration infection of children | |
| CN109394806B (en) | Processing method of ginseng and polygonatum odoratum | |
| CN105497156A (en) | Preparation method of fleeceflower root life-extending preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20160907 |