CN105918330A - New application of sirolimus in inhibition of plant alternaria solani - Google Patents
New application of sirolimus in inhibition of plant alternaria solani Download PDFInfo
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- CN105918330A CN105918330A CN201610348867.5A CN201610348867A CN105918330A CN 105918330 A CN105918330 A CN 105918330A CN 201610348867 A CN201610348867 A CN 201610348867A CN 105918330 A CN105918330 A CN 105918330A
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- rapamycin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
Abstract
The invention discloses application of sirolimus in inhibition of plant alternaria solani and further discloses a fungicide. The fungicide contains rapamycin or a mixture of the rapamycin and a chemical fungicide. A TOR protein inhibitor rapamycin used in clinical medicine is used for prevention and control research of plant early blight for the first time, it is found through the research that the rapamycin and the mixture of the rapamycin and the chemical fungicide can effectively inhibit the plant alternaria solani, a chemical rapamycin pesticide can play a synergistic effect to inhibit the plant alternaria solani, a very significative base is provided for research of a novel drug for preventing and controlling the early blight, the novel fungicide is wide in application prospect and market prospect, a new choice is provided for early blight prevention and control, the using amount of the chemical pesticide can be remarkably decreased, and the fungicide has the great significance on reduction of pesticide usage amount and delay of the resistance of insect pests and also has the great application value on maintenance of ecosystem balance and sustainable development.
Description
Technical field
The present invention relates to the new application of rapamycin, particularly relate to the new of rapamycin suppression plant early epidemic pathogenic bacteria
Purposes.
Background technology
Early blight, is also called " ring spot " or " summer epidemic disease ", belongs to fungal disease, and seedling stage, Adult plant are equal
Can fall ill, seedling stage falls ill, the raw crineous scab of the basal part of stem of seedling, the most sunken, has wheel stricture of vagina.Adult plant is fallen ill
Typically develop to top from lower blade.Initial stage blade is water stain shape dirty-green scab, after expansion rounded or
Irregular wheels stricture of vagina speckle, edge has light green color or yellow halo.Middle part tool concentric wheel stripe, moist seasonal disease minister
Going out the mould layer of black, cardinal symptom is that disease portion has (with one heart) to take turns stricture of vagina.Early epidemic pathogenic bacteria can infect various plants, is to make
Important pathogen on thing, its host range is various, in addition to infecting Fructus Lycopersici esculenti and Rhizoma Solani tuber osi, also can infect peppery
The plants such as green pepper, Fructus Solani melongenae, Flos Daturae.At present it has been reported that the pathogen causing plant early blight mainly have eggplant
Alternaric bacteria (Alternaria solani), alternaric bacteria (Alternaria alternate), A.interrupta, A.
Grandis, A.tenuissima, A.dumosa, A.arborescens and A.infectoria etc. 8 kinds, wherein
Sporulation is Dominantpathogen, this Pseudomonas Deuteromycotina, Hyphomycetes, alternaria nees fungus, ripe
Mycelia crineous, has barrier film and branch.
For the preventing and treating of early blight, following several ways is presently mainly used to carry out, i.e. physical control, change
Learn preventing and treating and Biological control.Chemical prevention is one of major measure of plant protection, and production prevents and treats early blight
Major measure be to spray antibacterial.As protective fungicide copper agent, Bordeaux mixture, Mancozeb, hundred
Bacterium is clear, difoltan etc.；Systemic fungicide Boscalid, Tebuconazole, Difenoconazole, Fluoxastrobin, corruption
Mould profit, RP-26019, CGA-173506 etc..The use of chemical pesticide, has certain production effect, but for a long time
Using chemical agent also to bring a series of problem, one of them is the drug resistance that constantly variation produces of pathogenic bacteria
Property, current early blight all creates resistance to many chemical pesticides.Further, since the use of chemical agent
The ecological disruption problem caused the most increasingly is paid close attention to by people, is badly in need of substituting the environment of chemical agent
Friendly disease control system.
Biological control, briefly cures the disease worm and curing the disease worm with bacterium with worm exactly.In biocontrol of plant disease
Conventional microorganism has antibacterial, yeast, actinomycetes and mycete.Due to the gene of antibacterial and metabolite relatively
For simply, therefore as biological prevention and control agent, there are the biggest potentiality using it.Biological control can overcome chemical agent to lead
The Drug resistance caused and problem of environmental pollution.Biological control research currently for early epidemic pathogenic bacteria has been achieved for
Some progress, as research finds, Trichoderma spp. metabolite has inhibitory action, reasonable employment green pus to alternaria solani sorauer
Pseudomonas alba there was no significant difference between preventive effect and the Mancozeb of early blight, pseudomonas fluorescens, Kazakhstan
The culturing filtrate of thatch Trichoderma spp. can effectively suppress the spore germination of alternaria solani sorauer, Bulbus Allii and the extractive form corm of Bulbus Allii Cepae
The suppression ratio of early blight A.alternata is reached more than 50%, and the research of Liang Ning etc. shows that bacillus subtilis exists
Prevent and treat and target has the biggest potentiality to be exploited.
TOR (Target ofRapamycin) is serine, the Serineprotein kinase guarded in a kind of evolution,
Promoted propagation and the growth of cell by integration nutrition and energy signal in fungus, animal, plant.TOR
Signal path is formed at animal embryo, separate living tissue activates, plant roots and the growth of leaf, bloom, old and feeble and
The control of life cycle all plays pivotal role.Studying discovery in recent years, TOR also has regulation ribose
The biosynthesis of body, promotion translation, metabolism regulation and the function of suppression autophagy.Skill is disturbed by RNA
Art disturbs vegeto-animal TOR gene or tor sudden change to can result in embryonic death, illustrates that TOR gene is planted dynamic
The growth and development dynamics of thing plays central role.
Rapamycin (Rapamycin, RAP), has another name called sirolimus (Sirolimus), in being a kind of macro ring
Esters immunosuppressant, is produced by streptomyces hygroscopicus, is an obligate generation of TOR (Target ofRapamycin)
Inhibitor.It is the most specific forms binary complex with FKBP12 protein binding, this complex again with
The FRB domain of TOR albumen combines, thus reaches to suppress the activity of TOR albumen.When FKBP12 base
During because undergoing mutation, will be substantially reduced or lose the binding ability with rapamycin so that rapamycin
Lose the activity of suppression TOR albumen.In terrestrial plant, FKBP12 gene there occurs adaptive sudden change,
Special being effectively combined can not be carried out with rapamycin, therefore rapamycin be shown resistance；But
In fungus and mammal, FKBP12 can effectively be combined formation binary complex with rapamycin, thus
Can suppress the activity of wherein TOR albumen, therefore rapamycin is demonstrated by the quickest by fungus and mammal
The character of sense.According to this characteristic, rapamycin can be as the potential source biomolecule pesticide of preventing and treating plant pest.
To rapamycin research the earliest mainly as hypotoxic antifungal drug, the most anti-white is read
Pearl bacterium, is currently mainly extensively used as to prevent the rejection of organ transplantation and treatment from self exempting from clinical medicine
The immunosuppressant of epidemic disease disease, utilizes this compound to yet there are no relevant report to the preventing and treating carrying out plant early blight
Road.
Summary of the invention
It is an object of the invention to provide the new use of a kind of thunder handkerchief enzyme element suppression plant early epidemic pathogenic bacteria for problem above
On the way.
Be the technical scheme is that for achieving the above object
Rapamycin application in suppression plant early epidemic pathogenic bacteria.
In this technical scheme, described rapamycin is used alone or is used in mixed way with fungus chemical bactericide.
Further, described fungus chemical bactericide is plant early epidemic pathogenic bacteria antibacterial.
Further, described plant is plant of Solanaceae.
A kind of fungus antibacterial, comprises the mixture of rapamycin or rapamycin and fungus chemical bactericide.
In this technical scheme, described fungus chemical bactericide is plant early epidemic pathogenic bacteria antibacterial.
As preferably, described plant early epidemic pathogenic bacteria antibacterial is the spirit of first downy mildew.
As preferably, the concentration of described rapamycin is 1～50nmol/L；The concentration of described first downy mildew spirit
It is 0.05～0.5mL/L.
The invention has the beneficial effects as follows: the present invention will be used for clinical medical TOR protein inhibitor thunder handkerchief first
Mycin, in the study on prevention of plant early blight, finds rapamycin, rapamycin and fungus by research
The mixture of chemical bactericide all can suppress plant early epidemic pathogenic bacteria effectively, and rapamycin chemical pesticide can be assisted
Same-action suppresses plant early epidemic pathogenic bacteria, provides significantly basis for preventing and treating early blight new drug research,
Being applied to having a extensive future of new type bactericide, market prospect is good, provides a kind of new choosing for preventing and treating early blight
Select, it is possible to the use of chemical pesticide is greatly reduced, to reducing Pesticide use, delaying the generation of pest resistance
Significant, the balance and sustainable development maintaining ecosystem is also had great using value.
Accompanying drawing explanation
Fig. 1 is the 12 days situation maps of early epidemic pathogenic bacteria colony growth processed through variable concentrations first downy mildew spirit；DMSO
For comparison.
Fig. 2 is the colony diameter schematic diagram of early blight pathogenic bacteria after variable concentrations first downy mildew spirit processes and cultivates 12 days.
Fig. 3 is the 12 days situation maps of early epidemic pathogenic bacteria colony growth processed through variable concentrations RAP；DMSO is comparison.
Fig. 4 is the colony diameter schematic diagram of early blight pathogenic bacteria after variable concentrations RAP processes and cultivates 12 days.
Fig. 5 is the 12 days situation maps of early epidemic pathogenic bacteria colony growth processed through variable concentrations RAP+ first downy mildew spirit；
DMSO is comparison.
Fig. 6 is the colony diameter signal of early epidemic pathogenic bacteria after variable concentrations RAP+ first downy mildew spirit processes and cultivates 12 days
Figure.
Fig. 7 is the synergism curve that RAP+ first downy mildew spirit combined treatment early epidemic pathogenic bacteria obtains；R:
Rapamycin；First: first downy mildew spirit.
Detailed description of the invention
Main agents used in the present invention:
Rapamycin (Rapamycin): purchased from Selleck Chemicals company of the U.S., is a kind of specific
MTOR inhibitors, catalog number (Cat.No.) S1039.
First downy mildew spirit is commercially available common pesticide, effective ingredient be 30% first downy mildew spirit, comprise 6% metalaxyl and
24% mould spirit.
Early blight bacterial strain alternaria solani is purchased from Beijing Culture Collection.
Solid medium in all treadmill tests is potato dextrose agar (Potato Dextrose
Agar, PDA).
Use the spirit of rapamycin (RAP), first downy mildew, rapamycin+first downy mildew spirit (both respectively
It is used in combination) experiment its inhibitory action to early epidemic pathogenic bacteria of detection:
Preparation PDA culture medium, sterilizing is placed in water bath with thermostatic control so that it is temperature reduces and is stable at 50 DEG C of left sides
The right side, adds medicament (DMSO or rapamycin or the spirit of first downy mildew or the rapamycin of appropriate volume
The spirit of+first downy mildew) after the final concentration that achieves the goal, it is down flat plate.After the thorough cooled and solidified of flat board, with punching
Device is beaten from the edge cultivating early epidemic pathogenic bacteria flat board (early epidemic pathogenic bacteria cultural method is carried out with reference to product description) and is taken
Diameter is about the early blight agar block of 5mm, is placed in plate center to be measured, trains in 25 DEG C of constant incubator dark
Support, observed an experimental phenomena, and Taking Pictures recording, continuous observation 12 days altogether every four days.
Embodiment 1. uses first downy mildew spirit individual processing early epidemic pathogenic bacteria
The Concentraton gradient arranging the spirit of first downy mildew is 0.05ml/L, 0.5ml/L and 1ml/L, and preparation contains respectively
The PDA culture medium of the first downy mildew spirit of these 3 kinds of variable concentrations, each process sets 3 repetitions.Cultivate 12
Fungus upgrowth situation is observed it appeared that (as shown in Figure 1, 2), along with the increasing of first downy mildew spirit concentration after it
Adding, fungus grows by being suppressed more and more obvious.The drug level of IC50 is 0.5ml/L.
Embodiment 2. uses Rapamycin individual processing early epidemic pathogenic bacteria
The Concentraton gradient arranging Rapamycin is 1nmol/L, 10nmol/L and 50nmol/L.Prepare respectively
The PDA culture medium of the Rapamycin containing these 3 kinds of variable concentrations, each process sets 3 repetitions.Cultivate
Fungus upgrowth situation is observed it appeared that (as shown in Figure 3,4), along with Rapamycin concentration after 12 days
Increasing, fungus grows by being suppressed more and more obvious.The drug level of its IC50 is about 50nmol/L.
Embodiment 3. rapamycin+first downy mildew spirit combined treatment early epidemic pathogenic bacteria
The concentration of rapamycin is respectively as follows: 1nmol/L, 10nmol/L, 50nmol/L；Making of first downy mildew spirit
It is 0.05ml/L and 0.5ml/L by concentration.By the concentration of above-mentioned rapamycin and the spirit of first downy mildew the most two-by-two
Combination processes early epidemic pathogenic bacteria, totally 6 combinations.The preparation thunder handkerchief containing these 6 kinds of variable concentrations combinations respectively
The PDA culture medium of mycin+first downy mildew spirit, each process sets 3 repetitions.Observe true after cultivating 12 days
Bacteria growing situation is it appeared that (as shown in Figure 5,6), along with rapamycin+first downy mildew spirit composite reagent
The increase of concentration, fungus grows by being suppressed more and more obvious.The drug level of IC50 is rapamycin:
50nmol/L+ first downy mildew spirit: 0.05ml/L.Rapamycin reaches after being applied in combination with pesticide first downy mildew spirit
To IC50, the concentration ratio of pesticide first downy mildew spirit reduces about 15 times when being used alone.Meanwhile,
(CI < 1 represents have association to obtain having cooperative effect between both medicines according to CompuSyn computed in software
Same-action；CI=1 represents have superposition；CI > 1 represents have antagonism), as shown in Figure 7.
Claims (8)
1. rapamycin application in suppression plant early epidemic pathogenic bacteria.
Application the most according to claim 1, it is characterised in that: described rapamycin be used alone or
It is used in mixed way with fungus chemical bactericide.
Application the most according to claim 2, it is characterised in that: described fungus chemical bactericide is plant
Early epidemic pathogenic bacteria antibacterial.
Application the most according to any one of claim 1 to 3, it is characterised in that: described plant is eggplant
Section plant.
5. a fungus antibacterial, it is characterised in that: comprise rapamycin or rapamycin and fungus chemistry
The mixture of antibacterial.
Fungus antibacterial the most according to claim 5, it is characterised in that: described fungus chemical bactericide
For plant early epidemic pathogenic bacteria antibacterial.
Fungus antibacterial the most according to claim 6, it is characterised in that: described plant early epidemic pathogenic bacteria kills
Microbial inoculum is the spirit of first downy mildew.
8. according to the fungus antibacterial described in claim 5 or 7, it is characterised in that: described rapamycin
Concentration is 1～50nmol/L；The concentration of described first downy mildew spirit is 0.05～0.5mL/L.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108676734A (en) * | 2018-09-03 | 2018-10-19 | 哈尔滨学院 | One plant of insect pathogenic fungus and its application |
| CN108676750A (en) * | 2018-05-29 | 2018-10-19 | 重庆大学 | A kind of streptomyces hygroscopicus producing salicylic acid and rapamycin and its application in prevention plant Oomycete and fungal disease |
| CN110468151A (en) * | 2019-09-04 | 2019-11-19 | 中国农业科学院都市农业研究所 | Fusarium oxysporum TOR gene RNAi carrier and its method for combining prevention and treatment dry rot of potato and wilt disease with salicylic acid |
| CN114568490A (en) * | 2022-04-01 | 2022-06-03 | 眉山职业技术学院(眉山技师学院) | Application of rapamycin in inhibiting growth of penicillium citrinum |
| CN115005220A (en) * | 2022-05-31 | 2022-09-06 | 中国热带农业科学院环境与植物保护研究所 | Application of rapamycin in inhibiting colletotrichum gloeosporioides or fusarium verticillioides |
| CN116836878A (en) * | 2023-07-19 | 2023-10-03 | 西南交通大学 | Pseudomonas fluorescens, microbial inoculum containing pseudomonas fluorescens, and preparation method and application thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108676750A (en) * | 2018-05-29 | 2018-10-19 | 重庆大学 | A kind of streptomyces hygroscopicus producing salicylic acid and rapamycin and its application in prevention plant Oomycete and fungal disease |
| CN108676750B (en) * | 2018-05-29 | 2021-11-16 | 重庆大学 | Streptomyces hygroscopicus capable of producing salicylic acid and rapamycin and application of streptomyces hygroscopicus in preventing and treating plant oomycetes and fungal diseases |
| CN108676734A (en) * | 2018-09-03 | 2018-10-19 | 哈尔滨学院 | One plant of insect pathogenic fungus and its application |
| CN108676734B (en) * | 2018-09-03 | 2021-08-06 | 哈尔滨学院 | Entomopathogenic fungi and application thereof |
| CN110468151A (en) * | 2019-09-04 | 2019-11-19 | 中国农业科学院都市农业研究所 | Fusarium oxysporum TOR gene RNAi carrier and its method for combining prevention and treatment dry rot of potato and wilt disease with salicylic acid |
| CN114568490A (en) * | 2022-04-01 | 2022-06-03 | 眉山职业技术学院(眉山技师学院) | Application of rapamycin in inhibiting growth of penicillium citrinum |
| CN114568490B (en) * | 2022-04-01 | 2023-12-19 | 眉山职业技术学院(眉山技师学院) | Application of rapamycin in inhibiting growth of penicillium citrinum |
| CN115005220A (en) * | 2022-05-31 | 2022-09-06 | 中国热带农业科学院环境与植物保护研究所 | Application of rapamycin in inhibiting colletotrichum gloeosporioides or fusarium verticillioides |
| CN116836878A (en) * | 2023-07-19 | 2023-10-03 | 西南交通大学 | Pseudomonas fluorescens, microbial inoculum containing pseudomonas fluorescens, and preparation method and application thereof |
| CN116836878B (en) * | 2023-07-19 | 2024-03-19 | 西南交通大学 | Pseudomonas fluorescens, microbial inoculum containing pseudomonas fluorescens, and preparation method and application thereof |
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