CN105911684A - Locating device used for target location of different microscopes and locating method thereof - Google Patents
Locating device used for target location of different microscopes and locating method thereof Download PDFInfo
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- CN105911684A CN105911684A CN201510524424.2A CN201510524424A CN105911684A CN 105911684 A CN105911684 A CN 105911684A CN 201510524424 A CN201510524424 A CN 201510524424A CN 105911684 A CN105911684 A CN 105911684A
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Abstract
The invention provides a locating device used for target location of different microscopes and a locating method thereof. The device comprises a locating glass slide and a multicolor strip scale plate. The multicolor strip scale plate is fixed on one main surface of the locating glass slide. The multicolor strip scale plate is provided with one or multiple scale identification areas. The scale identification areas comprise scale grids formed by main scale marks L1 and multicolor scale marks L2 in a staggered way. With application of the device, a target microscopic cutting area can be converted into concrete reading coordinates so that the target cutting area can be rapidly and accurately located in a laser microscopic cutting system and then the target can be rapidly and accurately searched, and thus laser cutting and other subsequent operation can be accurately performed.
Description
Technical field
The present invention relates to biological technical field, between different microscopes, carry out target location particularly to one
Positioner and localization method.
Background technology
Laser microprobe dating technology is to utilize laser to biological specimen (tissue, cell cluster, the list under microscope
Cell, chromosome, chromosome segment etc.) carry out the technology of contactless micro-dissections and separation.This technology is
The inverse gravity optical pressure of application launches the active collection realized sample, and whole process is without any Mechanical Contact, without heat
Damage, system can be accurately positioned, therefore, it is possible to guarantee to obtain sample is pollution-free, high-purity.Micro-dissections
Technology is one of important channel realizing accurate molecular biological analysis, is also to carry out DNA, RNA, egg at present
One of hot technology that white matter equimolecular biological study is the most commonly used.
Laser microdissection system includes microscopic system, under the microscope can be convenient, fast, accurately
The complete single cell of form or cell mass is obtained so that it is from group around from the natural tissues environment of cell
The pollution knitted, and can preferably retain surrounding tissue, in order to further investigation.Microdissection technology is specific
Playing an important role in the comparative studies of cell subsets, it is also presently the most and successfully solves cell in tissue
The technology of heterogeneity.At present this technology has succeeded application in multiple research fields, plants as moved
Thing gene expression analysis, the comparative studies of protein group, oncology studies, reproduction and embryonic development research,
Metabolite analysis etc..
Along with fast development and the extensive application of laser microprobe dating technology of molecular biology research, on market
Multiple micro-dissection system is arisen at the historic moment.But as high-grade, precision instrument, the micro-dissections of complete set
The market price of system is still within comparing higher level, it might even be possible to say that it belongs to expensive instrument and equipment, for general
Logical laboratory is difficult to be equipped with.Therefore, a set of micro-dissection system is usually multiple laboratory or research institution
Being shared, the even sample of different regions converges to carry out at micro-dissection system place detection etc., and this is the most right
Researcher proposes the requirement carrying out instrument reservation in advance, adds scientific research time cost.
Further, since the restriction of the equipment of outfit, in current micro-dissection system, the configuration of microscopic system is also
Being difficult to meet the Research Requirements of people, and a lot of micro-dissection system does not has coordinate scale, this is to showing
The scientific research personnel of micro-cutting operation searches target cutting zone and brings the biggest difficulty.And if by customization specially
The microscopic system reaching Research Requirements of door and the method being equipped with certain coordinate scale solve this difficulty, then
Can additionally increase the biggest one and be equipped with cost of equipment.
In view of the disadvantages described above of existing laser microprobe dating technology, this area in the urgent need to exploitation can simply,
Quickly, the positioner carrying out target location between different microscopes of low cost.
Summary of the invention
It is an object of the invention to provide a kind of simple, quickly, low cost between different microscopes, carry out mesh
Demarcate positioner and the localization method of position.
A first aspect of the present invention, it is provided that a kind of location dress carrying out target location between different microscopes
Put, including: location slide and a polychrome colour bar scale plate, wherein polychrome colour bar scale plate is fixed on and determines
On one first type surface of position slide, and the first type surface face that the area of polychrome colour bar scale plate is location slide
Long-pending 10%-90%；
Polychrome colour bar scale plate is provided with one or more scale cog region, and scale cog region includes by mass-tone scale
Line L1 and polychrome graduation mark L2 interlock constitute scale grid, and by " L1-L2 " arrangement mode be spaced
Arranged adjacent；
The color of polychrome graduation mark L2 is different from the color of mass-tone graduation mark L1, and polychrome graduation mark L2
Color category is 3-10 kind, and the distance between two mass-tone graduation mark L1 of arbitrary neighborhood is
0.01cm-0.5cm。
In another preference, described device is additionally provided with the scale for illustrating scale reading and measures
District is described, wherein said scale is measured explanation district and is arranged on the slide of described location or is arranged at described polychrome
On colour bar scale plate.
In another preference, the first type surface that area is described location slide of described polychrome colour bar scale plate
The 20%-80% of area.
In another preference, described polychrome graduation mark L2 constitutes on laterally and/or longitudinally one or more
The polychrome graduation mark circulation group being made up of polychrome graduation mark L2 described in 3-10 bar, and at each described polychrome
In graduation mark circulation group, described polychrome graduation mark L2 is arranged by identical color sequence.
In another preference, the color category of described polychrome graduation mark L2 is 3,4,5 or 6 kind.
In another preference, in a described scale cog region, two described polychromes of arbitrary neighborhood are carved
The distance between distance and the two of arbitrary neighborhood described mass-tone graduation mark L1 between degree line L2 is equal.
In another preference, the live width of described mass-tone graduation mark L1 and polychrome graduation mark L2 is any phase
The 1/50 to 1/4 of the adjacent distance between two described mass-tone graduation mark L1.
In another preference, described scale cog region is rectangle, circular or oval.
A second aspect of the present invention, it is provided that a kind of microslide kit, described kit includes n
Arbitrary described positioner in the specimen slide observed for microscope and first aspect present invention, and
The specification of the specification of described specimen slide and described location slide is identical, wherein n be 5-1000 just
Integer.
A third aspect of the present invention, it is provided that a kind of location side carrying out target location between different microscopes
Method, including step:
A specimen slide is positioned under the first microscope and observes by (), wherein place in specimen slide
Sample, sample is had to contain the target of pending laser microprobe dating；
B () finds the target of pending laser microprobe dating under the first microscope, and record object is first
Microscope coordinate readings under microscope, is designated as the first coordinate readings；
C () takes off specimen slide from the first microscope, and be replaced by described between different microscopes
Carry out the positioner of target location, the wherein specification positioning slide in positioner and specimen slide
Specification be consistent；
D (), based on positioner, determines first coordinate readings correspondence in the scale cog region of positioner
Coordinate, is designated as the second coordinate readings；
E () takes off positioner from the first microscope, and be positioned over by positioner and show for carrying out laser
Under second microscope of micro-cutting；
F () finds second coordinate readings correspondence position in scale cog region under the second microscope, determine
The field of view that target is corresponding in the second microscope；
G () takes off positioner from the second microscope, and be replaced by the sample load glass described in step (a)
Sheet so that target is positioned at the second microscopical field of view, thus realizes the location to target.
In should be understood that within the scope of the present invention, above-mentioned each technical characteristic of the present invention and below (as implemented
Example) in can be combined with each other between each technical characteristic of specifically describing, thus constitute new or preferred skill
Art scheme.As space is limited, the most tired at this state.
Accompanying drawing explanation
Fig. 1 is the structural representation of the positioner in embodiment 1；
Fig. 2 is the structural representation of the positioner in embodiment 2.
Detailed description of the invention
The present inventor, through extensively in-depth study, develops a kind of simple in construction, maneuverable first
For carrying out the positioner of target location between different microscopes, utilize this positioner can be rapidly and accurately
Carry out target-region locating, it is simple to after realizing the quickly lookup for single target tumour cell and it being carried out
Continuous operation (such as micro-dissections), so be easy to follow-up target to be analyzed (as to tumour unicellular complete outside
Aobvious son or Whole genome analysis), complete the present invention on this basis.
Term
As used herein, term " scale grid " refers to (include mass-tone scale at horizontal and vertical graduation mark
Line L1 and polychrome graduation mark L2) the staggered rectangular mesh constituted.
It should be noted that in the claim and specification of this patent, such as first and second or the like
Relational terms be used merely to not separate, and not an entity or operation with another entity or operating space
Necessarily require or imply relation or the order that there is any this reality between these entities or operation.And
And, term " includes ", " comprising " or its any other variant are intended to comprising of nonexcludability,
So that include that the process of a series of key element, method, article or equipment not only include those key elements, and
And also include other key elements being not expressly set out, or also include for this process, method, article or
The key element that person's equipment is intrinsic.In the case of there is no more restriction, statement " include one " and limit
Key element, it is not excluded that there is also other in including the process of described key element, method, article or equipment
Identical element.
Positioner
The invention provides a kind of positioner carrying out target location between different microscopes, this device bag
Include: location slide 1, polychrome colour bar scale plate 2.Wherein, polychrome colour bar scale plate 2 is fixed on location
On one first type surface of slide 1.
Preferably, described positioner also includes that the scale for illustrating scale reading measures explanation district
4, scale is measured explanation district 4 and is arranged on the slide 1 of location or is arranged on polychrome colour bar scale plate 2.
In the present invention, polychrome colour bar scale plate 2 is provided with one or more (such as 1-10, preferably 1-5
Individual, more preferably 1-3) scale cog region 3, each scale cog region 3 includes by mass-tone graduation mark L1 with many
Look graduation mark L2 interlock constitute scale grid, and by " L1-L2 " arrangement mode be spaced arranged adjacent.
In the present invention, typically, the color of polychrome graduation mark L2 is different from the color of mass-tone graduation mark L1,
And the color category of polychrome graduation mark L2 is 3-10 kind, and two mass-tone graduation mark L1 of arbitrary neighborhood
Between distance be 0.01cm-0.5cm.
In the present invention, the distance between two adjacent mass-tone graduation marks is not particularly limited.Generally, may be used
The distance defined between two adjacent mass-tone graduation marks is 1 (long measure) in reference axis, defines colorful
Distance between graduation mark and adjacent mass-tone graduation mark is 0.5 (long measure) in reference axis.
In the present invention, described polychrome graduation mark L2 can constitute on laterally and/or longitudinally one or more by
The polychrome graduation mark circulation group that 3-10 bar polychrome graduation mark L2 is constituted, and circulate at each polychrome graduation mark
In group, polychrome graduation mark L2 is carried out arranging (as repeated " blue-red-yellow-green-purple " by identical color sequence
Deng color sequence).
In the present invention, it is preferred to scale cog region 3 be rectangle, circular or oval.
In the present invention, it is preferred to, in a described scale cog region 3, two polychromes of arbitrary neighborhood
Distance between graduation mark L2 is equal with the distance between the two of arbitrary neighborhood mass-tone graduation mark L1, mass-tone
The live width of graduation mark L1 and polychrome graduation mark L2 is between two mass-tone graduation mark L1 of arbitrary neighborhood
The 1/50 to 1/4 of distance.
In another preference, the area of polychrome colour bar scale plate 2 is the main surface area of location slide 1
20%-80%.
In another preference, the color category of polychrome graduation mark L2 is not particularly limited, generally >=3 kinds.
Representative classes includes (but being not limited to): 3,4,5 or 6 kind.
In another preference, the color of polychrome graduation mark L2 is purple, green, yellow, red, blue, and polychrome
Graduation mark L2 constitutes identical polychrome graduation mark circulation group, wherein, polychrome graduation mark on horizontal and vertical
In circulation group, polychrome graduation mark L2 is by the color sequence arrangement of " purple-green-yellow-red-blue ".
In another preference, the live width of described mass-tone graduation mark and polychrome graduation mark is the two of arbitrary neighborhood
1/25 to 1/8 of distance between mass-tone graduation mark described in bar.
In another preference, described scale cog region 3 is rectangle.
In another preference, described polychrome colour bar scale plate 2 is provided with the scale of 2-3 arranged adjacent to be known
Other district 3.
In another preference, in the scale cog region 3 of described arranged adjacent, between mass-tone graduation mark L1
Distance be same or different (as in 3 scale cog regions 3, distance between each L1 is respectively
(i) 0.02,0.05 and 0.10cm；Or (ii) 0.05,0.10 and 0.15cm).
In the present invention, the material of location slide 1 is not particularly limited, it is ensured that printing opacity, Ke Yishi
The slide material of any employing.Representational material includes (but being not limited to): glass, plastics.Generally,
Described material is transparent, and is colourless or has certain color.Certainly, the present invention positions slide 1
Material can also be opaque or translucent.
Certainly, in another preference, the present invention positions the material of slide 1 and the material of specimen slide
It is identical or essentially identical.
The positioner of the present invention is expanded on further in conjunction with accompanying drawing.
A kind of typical positioner of the present invention is as shown in Figure 1.Positioner in Fig. 1 includes: location
Slide 1, polychrome colour bar scale plate 2 and scale measure explanation district 4.Wherein location slide 1 is
Cytelligen sheet glass, identical with the slide specification used by cell sample smear, scale measures explanation district
4 are arranged on the slide 1 of location, and polychrome colour bar scale plate 2 is fixed on a master meter of location slide 1
On face, polychrome colour bar scale plate 2 is provided with multiple scale cog region 3, and all multiple scale cog regions 3 are constituted
Long 1.5cm, the rectangle of wide 1.5cm.The color of the mass-tone graduation mark L1 in each scale cog region 3 is equal
For black, the color of polychrome graduation mark L2 is purple, green, yellow, red, blue, and polychrome graduation mark L2 is at horizontal stroke
Constituting identical polychrome graduation mark circulation group to upper, wherein, in polychrome graduation mark circulation group, polychrome is carved
Degree line L2 is by the color sequence arrangement of " purple-green-yellow-red-blue ", two mass-tone graduation marks of arbitrary neighborhood
Distance be 0.1cm, the distance of two polychrome graduation marks of arbitrary neighborhood is 0.1cm, arbitrary neighborhood
The distance of mass-tone graduation mark and polychrome graduation mark be 0.05cm.
The another kind of typical positioner of the present invention is as shown in Figure 2.Positioner in Fig. 2 includes: fixed
Position slide 1, polychrome colour bar scale plate 2 and scale measure explanation district 4.Wherein location slide 1 is Fig. 1
In location slide 1, scale measure explanation district 4 be arranged at location slide 1 on, polychrome colour bar scale
Sheet 2 is fixed on a first type surface of location slide 1, and polychrome colour bar scale plate 2 is provided with multiple scale to be known
Other district 3, all multiple scale cog regions 3 constitute long 4.5cm, the rectangle of wide 2.0cm.Each scale
The color of the mass-tone graduation mark L1 in cog region 3 is black, the color of polychrome graduation mark L2 be purple, green,
Yellow, red, blue, and polychrome graduation mark L2 constitutes identical polychrome graduation mark circulation group on horizontal and vertical,
Wherein, in polychrome graduation mark circulation group, polychrome graduation mark L2 presses the color sequence of " purple-green-yellow-red-blue "
Arrangement, the distance of two mass-tone graduation marks of arbitrary neighborhood is 0.1cm, and two polychromes of arbitrary neighborhood are carved
The distance of degree line is 0.1cm, and the mass-tone graduation mark of arbitrary neighborhood is with the distance of polychrome graduation mark
0.05cm。
Kit
Present invention also offers a kind of microslide kit, including n the sample observed for microscope
In this slide and described positioner, and the specification of specimen slide and described positioner
The specification of slide is identical, and wherein n is the positive integer of 5-1000, and preferably n is 10-200.
Localization method and subsequent operation
The positioner that present invention also offers a kind of present invention of utilization carries out target location and carries out follow-up
The method of operation (such as laser cutting), it includes step:
A specimen slide is positioned under the first microscope and observes by (), in wherein said specimen slide
Being placed with sample, described sample contains the target of pending laser microprobe dating；
B () finds the target of described pending laser microprobe dating under described first microscope, and record institute
State target microscope coordinate readings under the first microscope, be designated as the first coordinate readings；
C () takes off described specimen slide from described first microscope, and be replaced by described in difference
The positioner of target location is carried out, the described location slide 1 in wherein said positioner between microscope
Specification be consistent with the specification of described specimen slide；
D (), based on described positioner, determines described first coordinate readings described quarter at described positioner
Respective coordinates in degree cog region 3, is designated as the second coordinate readings；
E () takes off described positioner from described first microscope, and described positioner is positioned over use
Under the second microscope carrying out laser microprobe dating；
F () finds described second coordinate readings under described second microscope in described scale cog region 3
Correspondence position, determines the field of view that described target is corresponding in described second microscope；
G () takes off described positioner from described second microscope, and be replaced by described in step (a)
Specimen slide so that described target is positioned at described second microscopical field of view, thus realizes described
The location of target.
Generally, the second described microscope is the equipment for carrying out the operations such as laser cutting.By the present invention
Positioner, can position quickly and accurately the targeted target of the operation such as pending laser cutting (as
Cancer cell), and contribute to significantly reducing probability of makeing mistakes, the work of operating personnel therefore can be greatly improved
Efficiency also reduces working strength.
In another preference, the inventive method also includes: in step (f), records described second coordinate
Reading microscope coordinate readings under the second microscope, is designated as the 3rd coordinate readings.
In another preference, the method for the present invention also includes: in step (h), to described pending
The target of laser microprobe dating carries out laser microprobe dating.
In the present invention, applicable the inventive method carries out observing and carry out the sample of micro-dissections the most especially
Limiting, representational sample includes (but being not limited to): tissue samples, cell sample.
In another preference, preferred target includes tumour cell, normal cell.
In the present invention, described sample can come from various different source, representational source include (but
It is not limited to): mammal (such as people), bacterium, fungi, actinomyces, plant etc..
The method of the present invention can be diagnostic or nondiagnostic.In a class preference, the side of the present invention
Method is nondiagnostic and non-therapeutic.
Main advantages of the present invention include:
A () utilizes the positioner of the present invention to carry out target location can realize the quick lookup to target, than
The time of at least about 70%-90% has been saved in general lookup, improves operating efficiency.
B () is provided with the scale for illustrating scale reading and measures explanation district 4, it is simple to operator is quick
Read the corresponding coordinate of target.
C () makes the color difference of graduation mark adjacent on polychrome colour bar scale plate 2, it is simple to distinguish graduation mark.
D the distance between two adjacent mass-tone graduation marks of () definition is 1 in reference axis, define colorful quarter
Distance between degree line and adjacent mass-tone graduation mark is 0.5 in reference axis, it is simple to target label coordinate.
E the positioner of () present invention and method can be additionally used in coordinate position differentiated dissimilar micro-
The quick location of target area between mirror or same kind different brands microscope.
Below in conjunction with specific embodiment, the present invention is expanded on further.Should be understood that these embodiments are only used for
The bright present invention rather than restriction the scope of the present invention.The experiment side of unreceipted actual conditions in the following example
Method, generally according to normal condition, or according to the condition proposed by manufacturer.Unless otherwise indicated, otherwise
Percentage and number are percentage by weight and parts by weight.
Embodiment 1
The present embodiment uses the positioner shown in Fig. 1 to position target.In the present embodiment, target is thin
Born of the same parents are the tumour cell that DAPI is positive, CD45 is negative, CEP-8 is positive.Fluorescence 40 is just being put at Nikon 80i
Find target cell under power microscope, confirm that this cell is this cell to be moved to field of microscope after target cell
Centre, record microscope abscissa now and ordinate reading are (x:52.8, y:103.6), change
Above-mentioned positioner, by scale measure explanation district 4, read and record now target cell at polychrome colour bar
The corresponding coordinate of scale cog region 3 in scale plate 2, the self-defined coordinate of target cell can generate.
Zeiss in micro-dissection system is inverted under fluorescence 40 power microscope, and above-mentioned positioner is put load
Thing platform, after finding the self-defined coordinate of step, record microscope coordinate bit of target cell under this microscope
Put.
Above-mentioned positioner is taken off gently, puts sample slide, return to previous step note in micro-dissection system
The microscope coordinate position of record, has been quickly found out target cell.Follow-up this target cell can be further looked at
Or obtained by micro-dissections and unicellular to carry out unicellular full extron or Whole genome analysis etc..
Wherein, just putting from Nikon 80i and finding target cell to count fluorescence 40 power microscope, to micro-
The Zeiss of diced system is inverted under fluorescence 40 power microscope and is only found this target cell, and 6 bit manipulation personnel's is flat
All used times are 3 minute/cells, and compared with comparative example 1, the used time saves 98.57%.Target cell is searched
Success rate 100%, compared with comparative example 1, success rate is high by 75%.
Comparative example 1
Repeating embodiment 1, difference is, omits and uses positioner used in embodiment 1.
Result: just putting from Nikon 80i and finding target cell to count fluorescence 40 power microscope, to micro-
The Zeiss of diced system is inverted under fluorescence 40 power microscope and is only found this target cell, and 6 bit manipulation personnel's is flat
All used times are 3.5 hour/cells, wherein 4 bit manipulation people finder's fall short cell, target cell
Search success rate 25%.
Embodiment 2
The present embodiment uses the positioner shown in Fig. 2 to position target.In the present embodiment, A area
Certain research institute of colleges and universities new discovery sample has the cell of a specific type, need to be with the grinding of B area same type
Study carefully this newfound cell of mechanism's communication and discussion.
Certain research institute of colleges and universities of A area is coming 40 times of Microscopic observations of card microscope to target cell, records target
Cell is microscope coordinate (x:54, y:105) when field of microscope centre.Change above-mentioned positioner,
Measure explanation district 4 by scale, record now target cell scale identification in polychrome colour bar scale plate 2
The self-defined coordinate in district 3 is (x:10, y:4).Sample slide is existed with above-mentioned positioner and target cell
Self-defined coordinate (x:10, y:4) information in above-mentioned positioner together posts to B area studies mechanism.
B area studies mechanism is according to self-defined coordinate (x:10, the y:4) information of target cell, in its laboratory
The target cell self-defined coordinate (x:10, y:4) in above-mentioned positioner is found on Nikon microscope used,
Record now microscope coordinate readings (x:54.5, y:107.5).Change sample slide, return to microscope and sit
Cursor position, has been quickly found out target cell.
Wherein, B area studies mechanism is from taking sample slide and target cell information begins look for target cell
Counting, to finding this target cell only under Nikon microscope, the average used time of 6 bit manipulation personnel is 30 seconds.
Compared with comparative example 2, the used time saves 99.59%.
Comparative example 2
Repeating embodiment 2, difference is, omits and uses positioner used in embodiment 2.
Result: B area studies mechanism is from taking sample slide and target cell information begins look for target cell
Counting, to finding this target cell under Nikon microscope only, the average used time of 6 bit manipulation personnel is 2 little
Time.
The all documents mentioned in the present invention are incorporated as reference the most in this application, just as each document
It is individually recited as with reference to like that.In addition, it is to be understood that after the above-mentioned instruction content having read the present invention,
The present invention can be made various changes or modifications by those skilled in the art, and these equivalent form of values fall within this Shen equally
Please appended claims limited range.
Claims (10)
1. the positioner being used for carrying out target location between different microscopes, it is characterised in that bag
Include: location slide (1) and polychrome colour bar scale plate (2)；
Described polychrome colour bar scale plate (2) is fixed on a first type surface of described location slide (1), and institute
State the 10%-90% of the main surface area that area is described location slide (1) of polychrome colour bar scale plate (2)；
Described polychrome colour bar scale plate (2) is provided with one or more scale cog region (3), described scale cog region
(3) include being interlocked by mass-tone graduation mark L1 and polychrome graduation mark L2 the scale grid constituted, and presses
" L1-L2 " arrangement mode interval arranged adjacent；
The color of described polychrome graduation mark L2 is different from the color of described mass-tone graduation mark L1, and described polychrome
The color category of graduation mark L2 is 3-10 kind；
Further, the distance between two of arbitrary neighborhood described mass-tone graduation mark L1 is 0.01cm-0.5cm.
Positioner the most according to claim 1, it is characterised in that described device is additionally provided with use
Measuring explanation district (4) in the scale illustrating scale reading, wherein said scale is measured explanation district (4) and is set
It is placed in described location slide (1) above or to be arranged on described polychrome colour bar scale plate (2).
Positioner the most according to claim 1, it is characterised in that described polychrome colour bar scale plate (2)
The 20%-80% of the main surface area that area is described location slide (1).
Positioner the most according to claim 1, it is characterised in that described polychrome graduation mark L2 exists
Laterally and/or longitudinally the one or more polychrome being made up of polychrome graduation mark L2 described in 3-10 bar of upper composition is carved
Degree line circulation group, and in each described polychrome graduation mark circulation group, described polychrome graduation mark L2 presses phase
Same color sequence arranges.
5. according to described positioner arbitrary in claim 1-4, it is characterised in that described polychrome is carved
The color category of degree line L2 is 3,4,5 or 6 kind.
6. according to described positioner arbitrary in claim 1-4, it is characterised in that described in one
In scale cog region (3), the distance between two described polychrome graduation mark L2 of arbitrary neighborhood and arbitrary neighborhood
Two described mass-tone graduation mark L1 between distance equal.
7. according to described positioner arbitrary in claim 1-4, it is characterised in that described mass-tone is carved
The live width of degree line L1 and polychrome graduation mark L2 is between two described mass-tone graduation mark L1 of arbitrary neighborhood
Distance 1/50 to 1/4.
8. according to described positioner arbitrary in claim 1-4, it is characterised in that described scale is known
Other district (3) is rectangle, circular or oval.
9. a microslide kit, it is characterised in that described kit includes n for showing
Arbitrary described positioner in the specimen slide of micro mirror observation and claim 1-7, and described sample
The specification of the specification of this slide and described location slide (1) is identical, wherein n be 5-1000 just
Integer.
10. the method carrying out target location between different microscopes, it is characterised in that include step:
A specimen slide is positioned under the first microscope and observes by (), in wherein said specimen slide
Being placed with sample, described sample contains the target of pending laser microprobe dating；
B () finds the target of described pending laser microprobe dating under described first microscope, and record institute
State target microscope coordinate readings under the first microscope, be designated as the first coordinate readings；
C () takes off described specimen slide from described first microscope, and be replaced by described in claim 1
The positioner for carrying out target location between different microscopes, described in wherein said positioner
The specification of location slide (1) is consistent with the specification of described specimen slide；
D (), based on described positioner, determines described first coordinate readings described quarter at described positioner
Respective coordinates in degree cog region (3), is designated as the second coordinate readings；
E () takes off described positioner from described first microscope, and described positioner is positioned over use
Under the second microscope carrying out laser microprobe dating；
F () finds described second coordinate readings under described second microscope in described scale cog region (3)
Correspondence position, determine the field of view that described target is corresponding in described second microscope；
G () takes off described positioner from described second microscope, and be replaced by described in step (a)
Specimen slide so that described target is positioned at described second microscopical field of view, thus realizes described
The location of target.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510524424.2A CN105911684B (en) | 2015-08-24 | 2015-08-24 | A kind of positioning device and localization method for carrying out target positioning between different microscopes |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510524424.2A CN105911684B (en) | 2015-08-24 | 2015-08-24 | A kind of positioning device and localization method for carrying out target positioning between different microscopes |
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| CN105911684A true CN105911684A (en) | 2016-08-31 |
| CN105911684B CN105911684B (en) | 2018-07-24 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442324A (en) * | 2016-11-01 | 2017-02-22 | 昆明泰来光学科技开发有限公司 | Multi-waveband LED reflected/transmitted illumination objective table |
| CN110560930A (en) * | 2019-09-16 | 2019-12-13 | 深圳泰软软件科技有限公司 | Cutting method and device for blood spot of blood sampling card and readable storage medium |
| CN112798475A (en) * | 2020-12-22 | 2021-05-14 | 西安科技大学 | Method, system and device for monitoring diffusion area of grouting slurry in rock-soil mass |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1262566A2 (en) * | 2001-05-30 | 2002-12-04 | Agilent Technologies, Inc. | Composite polynucleotide arrays |
| CN2729704Y (en) * | 2004-09-07 | 2005-09-28 | 中国人民解放军第三军医大学 | Organisms macromolecule microarray positioner |
| CN201035215Y (en) * | 2007-01-19 | 2008-03-12 | 马青松 | Glass carrier special for pathological mechanism |
| CN202794697U (en) * | 2012-12-05 | 2013-03-13 | 崔文钰 | Slide glass sheet with scale |
| CN203037919U (en) * | 2013-01-25 | 2013-07-03 | 浙江师范大学 | Novel cover slip with mark |
| CN203054338U (en) * | 2013-01-08 | 2013-07-10 | 复旦大学 | Slide glass with grid reference lines |
| CN103627619A (en) * | 2012-08-23 | 2014-03-12 | 徐元诚 | Grid positioning sheet capable of detecting sperms quickly |
| CN205027969U (en) * | 2015-08-24 | 2016-02-10 | 上海宝藤生物医药科技股份有限公司 | A positioner that is used for carrying on between different microscopes object positioning |
-
2015
- 2015-08-24 CN CN201510524424.2A patent/CN105911684B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1262566A2 (en) * | 2001-05-30 | 2002-12-04 | Agilent Technologies, Inc. | Composite polynucleotide arrays |
| CN2729704Y (en) * | 2004-09-07 | 2005-09-28 | 中国人民解放军第三军医大学 | Organisms macromolecule microarray positioner |
| CN201035215Y (en) * | 2007-01-19 | 2008-03-12 | 马青松 | Glass carrier special for pathological mechanism |
| CN103627619A (en) * | 2012-08-23 | 2014-03-12 | 徐元诚 | Grid positioning sheet capable of detecting sperms quickly |
| CN202794697U (en) * | 2012-12-05 | 2013-03-13 | 崔文钰 | Slide glass sheet with scale |
| CN203054338U (en) * | 2013-01-08 | 2013-07-10 | 复旦大学 | Slide glass with grid reference lines |
| CN203037919U (en) * | 2013-01-25 | 2013-07-03 | 浙江师范大学 | Novel cover slip with mark |
| CN205027969U (en) * | 2015-08-24 | 2016-02-10 | 上海宝藤生物医药科技股份有限公司 | A positioner that is used for carrying on between different microscopes object positioning |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442324A (en) * | 2016-11-01 | 2017-02-22 | 昆明泰来光学科技开发有限公司 | Multi-waveband LED reflected/transmitted illumination objective table |
| CN110560930A (en) * | 2019-09-16 | 2019-12-13 | 深圳泰软软件科技有限公司 | Cutting method and device for blood spot of blood sampling card and readable storage medium |
| CN110560930B (en) * | 2019-09-16 | 2021-11-16 | 深圳泰软软件科技有限公司 | Cutting method and device for blood spot of blood sampling card and readable storage medium |
| CN112798475A (en) * | 2020-12-22 | 2021-05-14 | 西安科技大学 | Method, system and device for monitoring diffusion area of grouting slurry in rock-soil mass |
| CN112798475B (en) * | 2020-12-22 | 2024-06-07 | 中煤能源研究院有限责任公司 | Monitoring method, system and device for grouting slurry diffusion area in rock-soil body |
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