CN105911168A - Determination method of acrolein-DNA adduct - Google Patents

Determination method of acrolein-DNA adduct Download PDF

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CN105911168A
CN105911168A CN201610227445.2A CN201610227445A CN105911168A CN 105911168 A CN105911168 A CN 105911168A CN 201610227445 A CN201610227445 A CN 201610227445A CN 105911168 A CN105911168 A CN 105911168A
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dna
acr
solution
adduct
assay method
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CN105911168B (en
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侯宏卫
胡清源
陈欢
刘鲁娟
王红娟
朱贝贝
陈建
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National Tobacco Quality Supervision and Inspection Center
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography

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Abstract

A determination method of acrolein-DNA adduct is a determination method of alpha-Acr-dG and gamma-Acr-dG in DNA, and concretely comprises the following steps: exposing calf thymus DNA to an acrolein solution, carrying out enzyme hydrolysis on the obtained DNA solution, and sequentially adding a stable isotope internal standard substance and DNA hydrolase; and carrying out Strata-X column solid phase extraction on the obtained hydrolysate, collecting the obtained eluate, introducing the eluate to an LC-MS/MS system, and accurately detecting the content level of the alpha-Acr-dG and gamma-Acr-dG. The method is a brand new determination method of the acrolein-DNA adduct in DNA, the stable isotope is adopted as an internal standard quantitative analysis substance in order to reduce the error caused by a pretreatment process, and tandem mass spectrometry well improves the selectivity, the accuracy and the sensitivity of the method. The method well improves the chromatographic separation process, shortens the chromatography time and reduces the consumption of an organic solvent through selecting a chromatographic column and optimizing the eluting conditions.

Description

A kind of assay method of acrylic aldehyde-DNA adduct
Technical field
The invention belongs to the physical and chemical inspection technical field of DNA sample, relate generally to 6-hydroxyl-1 in DNA, N2-propanol-2'- Guanine (α-Acr-dG) and 8-hydroxyl-1, N2The determination techniques field of-propanol-2'-guanine (γ-Acr-dG) adduct, Specifically one uses liquid chromatograph-electrospray ionisation-tandem mass spectrograph (LC-MS/MS) to measure acrylic aldehyde-DNA in DNA The method of adduct.
Background technology
Acrylic aldehyde is a kind of pollutant being widely present in environmental matrices.They occur mainly with organic not exclusively Burning, such as commercial production, cigarette smoke, motor-vehicle tail-gas and cooking fume etc..Active aldehyde radical needs not move through organism metabolism Just can nucleophilic group in direct aggression organism, as the guanine in nucleic acid, adenine, cytosine and thymus pyrimidine are formed DNA adduct.The main DNA adduct that acrylic aldehyde is formed includes 6-hydroxyl-1, N2-propanol-2'-guanine (α-Acr-dG) and 8-hydroxyl-1, N2-propanol-2'-guanine (γ-Acr-dG).Both DNA adducts all can cause G → T to suddenly change.
Currently, with respect to DNA adduct analyzing detecting method report more, mainly have32After P-, labelling technique, enzyme connection are exempted from Epidemic disease technology (ELISA), high performance liquid chromatography (HPLC-UV) and liquid chromatography-tandem mass spectrometry technology (LC-MS/MS).Wherein LC-MS/MS is widely used in the detection analysis of DNA adduct due to its higher sensitivity and selectivity.
Summary of the invention
The purpose of the present invention is based on above-mentioned prior art situation and uses liquid chromatography-tandem mass spectrometry (LC-MS/MS) The assay method of acrylic aldehyde-DNA adduct (α-Acr-dG and γ-Acr-dG) that technology is set up.The method has quick, accurate Really, sensitivity high, can be used for the qualitative and quantitative analysis of acrylic aldehyde-DNA adduct in DNA.
It is an object of the invention to be achieved through the following technical solutions:
A kind of assay method of acrylic aldehyde-DNA adduct, i.e. α-Acr-dG and the assay method of γ-Acr-dG in DNA, specifically Step is as follows:
A, acrylic aldehyde expose calf thymus DNA: take 400 μ g calf thymus DNAs, add the acrolein solution (acrylic aldehyde prepared The solution PBS solution of 0.1 M is prepared, concentration 0.001-1 mM), matched group adds the PBS solution of equal volume.By above-mentioned molten Liquid puts into 24 h in 37 DEG C of constant incubators；Use the ice ethanol precipitation DNA of 2 times of volumes and terminate reaction, being centrifuged and remove supernatant Liquid, the ethanol water with 70% cleans DNA sample, and the DNA agglomerate obtained dries and adds 200 μ L ultra-pure waters and redissolves, and uses NanoDrop 2000 ultramicrospectrophotometer detects the content of DNA at 260nm, for one absorbance units phase of double-stranded DNA When in 50 μ g/mL.
The enzyme hydrolysis of b, DNA and Solid-Phase Extraction (SPE): above-mentioned DNA is dissolved in the 10 mM Tris-HCl/5 mM of 500 μ L MgCl2In buffer (pH=7), add 20 μ L and mix internal standard, add 60U deoxyribonuclease Ⅰ, in 37 DEG C, hatch 2h, It is subsequently added 0.008U Phosphodiesterase I and 30U alkali phosphatase continues to hatch 2h in 37 DEG C.Hydrolyzed solution is lived through 1 mL methanol Change and the Strata-X solid phase extraction column of 1 mL pure water equilibrium, with 1 mL ultra-pure water and the methanol-water drip washing of 1 mL 10%, Afterwards with the methanol-water eluting of 1 mL 50%, collect eluent and be sample liquid to be measured.The purpose of this process is to make double-stranded DNA Enzymolysis becomes mononucleotide state, is blown the DNA adduct concentrated and purified needed for separating and being enriched with by Solid-Phase Extraction and nitrogen.Described mixed Be designated as in conjunction 100 ng/mL [15N5] α-AcrdG and [15N5]γ-AcrdG。
C, the preparation of standard working solution: α-Acr-dG and the γ-Acr-dG standard work with methanol preparation variable concentrations is molten Liquid.
D, liquid chromatography-tandem mass spectrometry (LC-MS/MS) measure, and draw the variable concentrations hybrid standard work prepared molten Liquid, injects LC-MS/MS system, obtains equation of linear regression, and liquid to be measured to sample is measured, and records analyte and internal standard peak The ratio of area, substitutes into unary linear regression equation, tries to achieve the content of analyte in sample liquid to be measured.
Chromatographic condition: choose Extend-C18(1.8 μm, 4.6 × 100 mm) chromatographic column, flow visualizing chooses A: 0.01% formic acid methanol and B:10 mM ammonium acetate solution, elution requirement is gradient elution: 0-2 min:25% A, 2-7 min: 35% A, 7-10 min:25% A；Flow velocity is 0.38 mL/min.Analysis time is 10 min, and sample size is 5 μ L.
Mass Spectrometry Conditions: electric spray ion source (ESI), multiple-reaction monitoring cation scan mode；Spray voltage: 5500 V, Ion source temperature: 600 DEG C, the amount of gas curtain gas is 20 psi, and atomization gas is 36 psi, and dry gas is 46 psi, and collision gas is 9 Psi, injects voltage: 10 V, impact energy: 9 eV, residence time: 100 ms.Monitoring ion pair be α-Acr-dG:324.3 → 208.2 quota ions pair, 324.3 → 190.1 qualitative ion pairs；Internal standard [15N5] α-Acr-dG is 329.2 → 213.2；γ- Acr-dG:324.3 → 208.1 quota ion pair, 324.3 → 190.2 qualitative ion pairs；Internal standard [15N5] γ-Acr-dG is 329.2→213.1。
Each analyte and interior target MRM parameter are shown in Table 1.Analysis process is by Applied Biosystems Analyst Version 1.5.1 software controls.
Analyte and interior target MRM parameter thereof under table 1 multiple-reaction monitoring pattern
* it is quota ion
The range of linearity of the inventive method and detection limit:
Series standard working solution is injected LC-MS/MS, obtains standard working curve, equation of linear regression and correlation coefficient. The point of α-Acr-dG and γ-Acr-dG standard curve is 0.02,0.05,0.1,0.2,0.5,1.0,1.5,2.0,5.0 and 10 Ng/mL, internal standard [15N5] α-AcrdG and [15N5] concentration of γ-AcrdG is 2 ng/mL.Object linear good, phase relation Number is all higher than 0.9996.Mark-on dilution is utilized to obtain quantitative limit and the detection limit of method, corresponding dense of ten times of signal to noise ratios of target peak Degree is for quantitative limit, and concentration corresponding to three times of signal to noise ratios is detection limit.The standard curve of analyte and quantitative limit and detection limit are shown in Table 2。
The standard working curve of table 2 object and LOD and LOQ
The inventive method recovery of standard addition and repeatability:
Take calf thymus DNA sample adds the recovery of standard addition of calibration method preparation method, altogether have chosen three and add water Flat, each pitch-based sphere replication 3 times, the response rate of the method obtained and repeatability, the results are shown in Table 3.The recovery of object Rate is between 97.3%-105.0%, and RSD is less than 5%, it was demonstrated that the accuracy of method and repeatability result are preferable.
The recovery of standard addition of table 3 analyte and repeatability
The method of the present invention overcomes the deficiency of prior art sample treatment, the chromatograph of acrylic aldehyde-DNA adduct in DNA Condition is optimized, and is optimized the coherent detection condition of LC-MS/MS.Compared with prior art the inventive method There is following excellent results:
1. compare with traditional liquid-phase chromatography method, owing to have chosen tandem mass spectrum so that selectivity and the sensitivity of method carry Height, is more beneficial for the mensuration of low content acrylic aldehyde-DNA adduct in DNA.
2. this method has easy and simple to handle, quick, accurate, sensitivity and reproducible advantage.
3. the inventive method uses stable isotope can reduce in sample pretreatment process as uantitative analytical thing The error caused, tandem mass spectrometry preferably improves selectivity and accuracy and the sensitivity of method.By chromatographic column and The selection and optimization of elution requirement, preferably improves chromatographic separation process, shortens the time of chromatography, decreases organic The consumption of solvent.
Accompanying drawing explanation
Fig. 1 α-Acr-dG and γ-Acr-dG and in be marked on 100 μMs of acrolein solution, the chromatograph of 37 DEG C of contamination 24h Figure.
Under Fig. 2 difference acrylic aldehyde reconditioning, α-Acr-dG and γ-Acr-dG content in calf thymus DNA.
Detailed description of the invention
The present invention is described further below in conjunction with example, but is not to limit the present invention.
The assay method of acrylic aldehyde-DNA adduct in a kind of DNA, its test process is to calf breast by acrolein solution Gland DNA exposes, and DNA solution carries out enzyme hydrolysis and adds stable isotope internal standard and DNA hydrolytic enzyme.Hydrolyzed solution warp The little column solid phase extraction of Strata-X, collects eluent, introduces LC-MS/MS systematic analysis.
Example 1:
1. instrument and reagent:
AB SCIEX triple quadrupole rods tandem mass spectrometry instrument (California, USA Applied Biosystems, Inc.), Agilent 1200 is efficient Liquid chromatograph (Agilent company of the U.S.), NanoDrop 2000 ultramicrospectrophotometer (Sai Mofei scientific & technical corporation of the U.S.), permanent Temperature incubator (Sai Mofei scientific & technical corporation of the U.S.), Milli-Q water purification system (Merck KGaA group), Analyst 1.5.1 number According to gathering and processing software.
Deoxyribonuclease Ⅰ, alkali phosphatase (U.S.'s knob Great Britain biotech company), Phosphodiesterase I, acetic acid Ammonium, formic acid and calf thymus DNA (Sigma of the U.S.), methanol (De Shan Reagent Company of Korea S), Strata-X polymer is little Post (33 μm, 30 mg/1mL, Guangdong Féraud door scientific instrument company limited).Standard substance α-Acr-dG, γ-Acr-dG and interior Mark thing [15N5]α-Acr-dG 、[15N5]γ-Acr-dG.Reagent is chromatographically pure.
2. sample treatment:
Acrylic aldehyde exposes calf thymus DNA: takes 400 μ g calf thymus DNAs, adds the acrolein solution of 1 mL variable concentrations (0.001,0.01,0.05,0.1,0.5 and 1.0 mM), matched group adds the PBS solution of equal volume.Above-mentioned solution is put into 24 h in 37 DEG C of constant incubators；Use the ice ethanol precipitation DNA of 2 times of volumes and terminate reaction, being centrifuged and remove supernatant, use The ethanol water of 70% cleans DNA sample, and the DNA agglomerate obtained dries and adds 200 μ L ultra-pure waters and redissolves, and uses NanoDrop 2000 ultramicrospectrophotometers detect the content of DNA at 260nm, are equivalent to 50 μ for one absorbance units of double-stranded DNA g/mL。
The enzyme hydrolysis of DNA and Solid-Phase Extraction (SPE): above-mentioned DNA is dissolved in the 10 mM Tris-HCl/5 mM of 500 μ L MgCl2In buffer (pH=7), add 20 μ L and mix internal standard, add 60U deoxyribonuclease Ⅰ, in 37 DEG C, hatch 2h, It is subsequently added 0.008U Phosphodiesterase I and 30U alkali phosphatase continues to hatch 2h in 37 DEG C.Hydrolyzed solution is lived through 1 mL methanol Change and the Strata-X solid phase extraction column of 1 mL pure water equilibrium, with 1 mL ultra-pure water and the methanol-water drip washing of 1 mL 10%, Afterwards with the methanol-water eluting of 1 mL 50%, collect eluent and be sample liquid to be measured.It is designated as 100 ng/mL's in described mixing [15N5] α-AcrdG and [15N5]γ-AcrdG。
3. assay method:
Each 5 L of calf thymus DNA sample after the standard solution of absorption variable concentrations and pre-treatment, inject LC-MS/MS system System carries out separating to be analyzed, and records the content of acrylic aldehyde-DNA adduct in sample.
Example 2: as described in Example 1, utilize method that this research sets up to variable concentrations acrylic aldehyde (0.001,0.01, 0.05,0.1,0.5 and 1.0 mM) acrylic aldehyde-DNA adduct has carried out detecting that (each sample is put down in the calf thymus DNA that exposes Row detection three times).Establishing criteria curve and peak area draw the concentration of DNA adduct in each sample, according to formula
In calculating each sample of acquisition, DNA adduct is relative to the content of nucleotide, and result is multiplied by 108It is converted into DNA adduct Number/108Individual nucleotide.The amount of data result display α-Acr-dG and γ-Acr-dG becomes effect to close with acrylic aldehyde reconditioning System (SPSS, P ＜ 0.005, Fig. 2).

Claims (6)

1. 6-hydroxyl-1 in an assay method for acrylic aldehyde-DNA adduct, i.e. DNA, N2-propanol-2'-guanine (α-Acr- And 8-hydroxyl-1, N dG)2The assay method of-propanol-2'-guanine (γ-Acr-dG), it is characterised in that: include in detail below Step:
A, acrylic aldehyde expose calf thymus DNA: take 400 μ g calf thymus DNAs, add the acrolein solution prepared, matched group Add the PBS solution of equal volume；Above-mentioned solution is put into 24 h in 37 DEG C of constant incubators；Use the ice ethanol of 2 times of volumes Precipitation DNA also terminates reaction, is centrifuged and removes supernatant, and the ethanol water with 70% cleans DNA sample, and the DNA agglomerate obtained dries in the air Dry doubling adds 200 μ L ultra-pure waters and redissolves, and detects containing of DNA with NanoDrop 2000 ultramicrospectrophotometer at 260nm Amount, is equivalent to 50 μ g/mL for one absorbance units (OD) of double-stranded DNA；
The enzyme hydrolysis of b, DNA and Solid-Phase Extraction (SPE): above-mentioned DNA is dissolved in the 10 mM Tris-HCl/5 mM of 500 μ L MgCl2In buffer, add 20 μ L and mix internal standard, add 60U deoxyribonuclease Ⅰ, in 37 DEG C, hatch 2h, add subsequently Entering 0.008U Phosphodiesterase I and 30U alkali phosphatase and continue to hatch 2h in 37 DEG C, hydrolyzed solution is through 1 mL methanol activation and 1 The Strata-X solid phase extraction column of mL pure water equilibrium, with 1 mL ultra-pure water and the methanol-water drip washing of 1 mL 10%, finally with 1 The methanol-water eluting of mL 50%, collects eluent and is sample liquid to be measured；
C, the preparation of standard working solution: with α-Acr-dG and the γ-Acr-dG standard working solution of methanol preparation variable concentrations；
D, liquid chromatography-tandem mass spectrometry (LC-MS/MS) measure, and draw the variable concentrations hybrid standard working solution prepared, note Entering LC-MS/MS system, obtain equation of linear regression, liquid to be measured to sample is measured, and records analyte and internal standard peak area Ratio, substitutes into unary linear regression equation, tries to achieve the content of analyte in sample liquid to be measured.
The assay method of acrylic aldehyde-DNA adduct the most according to claim 1, it is characterised in that: the propylene in step a The aldehyde solution PBS solution of 0.1 M is prepared, concentration 0.001-1 mM.
The assay method of acrylic aldehyde-DNA adduct the most according to claim 1, it is characterised in that: described in step b Be designated as in mixing 100 ng/mL [15N5] α-AcrdG and [15N5]γ-AcrdG。
The assay method of acrylic aldehyde-DNA adduct the most according to claim 1, it is characterised in that: step d have chosen Extend-C18 chromatographic column, specification 1.8 μm, 4.6 × 100 mm, flow visualizing chooses 0.01% formic acid methanol A and 10 mM second Acid aqueous ammonium B, gradient elution, analysis time is 10 min, and sample size is 5 μ L.
The assay method of acrylic aldehyde-DNA adduct the most according to claim 4, it is characterised in that: condition of gradient elution has Body is: 0-2 min:25% A, 2-7 min:35% A, 7-10 min:25% A；Flow velocity is 0.38 mL/min.
The assay method of acrylic aldehyde-DNA adduct the most according to claim 1, it is characterised in that: matter of connecting in step d The condition of spectrum detector: electric spray ion source (ESI), multiple-reaction monitoring cation scan mode；Spray voltage: 5500 V, from Source temperature: 600 DEG C, the amount of gas curtain gas is 20 psi, and atomization gas is 36 psi, and dry gas is 46 psi, and collision gas is 9 Psi, injects voltage: 10 V, impact energy: 9 eV, residence time: 100 ms；Monitoring ion pair be α-Acr-dG:324.3 → 208.2 quota ions pair, 324.3 → 190.1 qualitative ion pairs；Internal standard [15N5] α-Acr-dG is 329.2 → 213.2；γ- Acr-dG:324.3 → 208.1 quota ion pair, 324.3 → 190.2 qualitative ion pairs；Internal standard [15N5] γ-Acr-dG is 329.2→213.1。
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US20140017672A1 (en) * 2007-10-31 2014-01-16 Rebecca HOLMBERG Method and kit for purifying nucleic acids
CN104502492A (en) * 2015-01-14 2015-04-08 武汉大学 Chemical derivatization method and application thereof to nucleic acid modification detection by liquid chromatogram-mass spectrometer (LC-MS) method

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Title
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