CN105902643A

CN105902643A - Drug for preventing thrombus formation and treating thrombus and preparation method thereof

Info

Publication number: CN105902643A
Application number: CN201510766829.7A
Authority: CN
Inventors: 李明
Original assignee: Individual
Current assignee: Individual
Priority date: 2015-11-05
Filing date: 2015-11-05
Publication date: 2016-08-31

Abstract

The invention provides a drug for preventing thrombus formation and treating thrombus and a preparation method thereof. The drug for preventing thrombus formation and treating thrombus is a compound active part extracted from geum japonicum. The compound active part comprises at least one of polysacharide compounds, polyphenol compounds, flavonoid compounds and triterpene compounds. The compound active part is extracted from geum japonicum and acts on the human body so that thrombus formation is prevented and formed thrombus is treated. Through inhibiting thrombus formation and dissolving thrombus, the compound active part can prevent and treat thrombus formation-caused diseases in different tissue or organs.

Description

A kind of antithrombotic, the medicine treating thrombosis and preparation method thereof

Technical field

This patent relates to pharmaceutical field, particularly relate to a kind of antithrombotic, treatment thrombosis medicine and Preparation method.

Background technology

Thrombosis is formed and refers to that the visible component in blood occurs abnormal blood clot in blood circulation, or Hypostasis thing is there is in person in blood vessel wall and wall of the heart.Most common intentionally cerebral thromboembolism disease is One of lethal major reason disabled in world population.Other also have venous thrombosis and then cause Pulmonary infarction, portal vein embolization cause portal hypertension, table shallow property thrombophlebitis, lower limb Deep vein blood Bolt formation, Mesenteric arterial embolism and limb artery embolization etc..Blood circulation thrombosis mode substantially may be used It is divided into: 1. expose under inner membrance and allogenic material makes platelet aggregation activate；2. fibrin deposition.Typically Artery thrombosis, commonly referred to white thrombus, be mainly made up of platelet and fibrin, generally The blood vessel wall blood flow is very fast is occurred to have the position of damage, or aberrant angiogenesis position.Platelet is only attached on In the blood vessel wall of pathological changes, form platelet thrombus, fibrin when blood passes through, can be had to be partially formed, And it is attached to platelet thrombus surface.On fiber protein yarn, the thrombin of residual can make again blood in blood flow little Plate is attached on fiber protein yarn, and result forms platelet and fibrin covers the most repeatedly, Fiber protein yarn can also catch some red blood cells and leukocyte.Platelet thrombus is gradually grown up, and finally closes Plug blood vessel, blocks tissue or organ ischemia, anoxia that this tremulous pulse is arranged, occurs serious ischemic to damage Wound.Clinic is modal myocardial ischemia, infarction, cerebral embolism, Mesenteric arterial embolism and limbs Arterial thrombosis etc..Thrombus breaks loose can enter less intra-arterial with artery blood flow and cause thromboembolism, is common in Brain, spleen, renal infarction.Artery thrombosis and thromboembolism are the arterial occlusion sexually transmitted disease (STD)s caused by two kinds of different pathogenies Become.

Also having a kind of common thrombotic disease is microthrombusis in microcirculation thrombosis and blood.Betide micro-following Thrombosis in the venule of ring, microcirculation and blood capillary, can only the most just be found, therefore claims Microthrombus.Such as, during DIC, blood coagulation system is activated, and in whole body blood capillary, microthrombus is extensively formed, and causes Ischemic organ's dysfunction.These microthrombus major parts are fibrinous thrombus, also can be platelet Property thrombosis, or claim hyaline thrombus.Majority is partially formed in blood capillary, it is also possible to comes off and flows to occur at it Thromboembolism.Microvascular thrombosis can cause intravascular coagulation due to microcirculation disturbance；Also can be blocked up by the embolus come off Plug thin vessels, or because some factor coup injury capillary endothelium causes fibrin deposition.At hat In shape tremulous pulse, the data of becoming celestial shows, blocking property coronary artery thrombosis incidence rate is 15%～95%.Micro- Thrombosis is the most common in becoming celestial, and up to 37.6%, is more common in lung, liver, kidney and brain.In coagulant factor Effect and trigger cofactor participation under, fibrin deposition and platelet aggregation can be caused, twisted Agglomerating formation microthrombus and block blood capillary.The platelet of cohesion discharges again coagulant, activates thrombin, Promote fibrinous formation and deposition further, may result in disseminated inravascular coagulation.If fibrin It is deposited on erythrocyte surface, can hold mutually in cyclic process, adhere and form bigger grumeleuse, from Blood capillary extends to venule, causes the blood flow obstruction of local.When the erythrocyte being wrapped gradually occurs Regression, decomposition, the hemoglobin discharged and thromboplastin, blood coagulation can be promoted again, increase further Add the size of thrombosis.

Thrombotic disease is currently to endanger human health, causes one of reason that case fatality rate is the highest, such as hat The myocardial infarction that shape artery thrombosis or thromboembolism cause, cerebral thrombosis, cerebral embolism, deep venous thrombosis Formed, pulmonary infarction, limbs thromboembolism etc..Clinical treatment method mainly has antithrombotic and by The thromboembolism formed.Thromboembolism treatment has work of crucial importance to thrombosis and the thromboembolism of vitals With.Its objective is to dissolve as early as possible the thrombosis of internal vitals (such as brain, heart, lung etc.) tremulous pulse, promote Enter revascularization, the function of protection ischemia organ, reduce the case fatality rate of disease.Such as, clinical research table Bright, thromboembolism treatment applies best results in acute myocardial infarction shows effect 6 hours.But, going out of thromboembolism treatment Blood complication, especially cerebral hemorrhage are the major reasons limiting thromboembolism treatment.The preventing and treating mesh of thrombotic disease Be improve hypercoagulability, dredge or rebuild blood stream access, to prevent tissue ischemia, necrosis again.Anti- The measure of controlling generally includes anticoagulant therapy, thrombolytic therapy, anti-platelet therapy etc..Infarction is depended in prognosis The size of scope, situation of setting up and the treatment of Doppler flow mapping are the most timely.

Heparin, Low molecular heparin, aspirin, hirudin etc. are the medicines that anticoagulant therapy is conventional, can subtract Insufficiency of blood slurry viscosity and the effect of inhibition thrombosis.Additionally specific thrombin inhibitor is single chain polypeptide, Its anticoagulation participates in without Antithrombin III, Antithrombin Ⅲ II.Experiment shows except directly suppressing blood Bolt forms outer also suppression plaque factor expression, hence it is evident that after alleviating percutaneous transluminal coronary angioplasty (PTCA) Thrombus source restenosis, in its suppression thrombosis increases and makes arteria coronaria, remaining thromboembolism is more more effective than heparin. In addition to anticoagulant therapy, dissolving the thrombosis formed also is an important indispensable therapeutic choice. Thrombolytics, regardless of originating, is typically plasminogen activator.Thrombolytics mainly has streptokinase, urine Kinases, tissue-type plasminogen activator's (t-PA, serineprotein kinase) and stqphylokinase.

In a word, although we for the understanding of thrombosis relevant disease in arteriovenous or the chambers of the heart and treatment Recent decades has been achieved with considerable significant progress, but, suffer the people that various thrombotic disease is invaded and harassed Number is the highest, and presents ascendant trend with human society entrance aging society at a quick pace.And currently may be used Though treatment thrombotic disease have an above-mentioned multiple therapeutic scheme, but these platelet suppressant drugs, anti- It is dangerous that solidifying and thromboembolism treatment scheme has relatively massive hemorrhage, especially topmost complication in thromboembolism treatment It is hemorrhage, gastrointestinal tract, intracranial hemorrhage etc. will be watched out for especially.Therefore, thrombolytic specially good effect has been researched and developed , and the new therapeutic strategy of the tendency that do not causes bleeding is imperative.

Summary of the invention

The invention provides medicine of a kind of antithrombotic, treatment thrombosis and preparation method thereof, this Bright medicine is by the compound activity position extracted from Herba Gei, and gives human body by active site, Can realize the formation of pre-preventing thrombosis and treat the thrombosis formed, this active site is mainly by suppression The thrombosis that thrombosis and dissolving have been formed, thus reach prevention and treatment in different tissues or organ The various disease state that thrombosis causes.Concrete technical scheme is as follows:

A kind of antithrombotic, the medicine for the treatment of thrombosis, described medicine is to extract from Herba Gei Compound activity position；Described compound activity position include polysaccharide compound, polyphenol compound, At least one in flavone compound and triterpenoid compound.

Above-mentioned medicine, wherein, described compound activity position includes polyphenol compound, triterpenes At least one in compound.

Above-mentioned medicine, wherein, by being orally administered to the individual compound activity extracted from Herba Gei Antithrombotic or treatment thrombosis are come in position.

Above-mentioned medicine, wherein, gives individual from soft by subcutaneous injection, intramuscular injection or venoclysis Antithrombotic or treatment thrombosis are come in the compound activity position that hair Fructus Adinae extracts.

Above-mentioned medicine, wherein, described in the amount that gives be 0.1mg-8g compound activity position/kilogram Whose body weight/sky.

Above-mentioned medicine, wherein, described antithrombotic, treatment thrombosis include tissue or organ tremulous pulse, Tissue that in vein, blood capillary, in thrombosis, blood, thrombosis causes or organ ischemia or blood Supply discontinuity.

The another side of the present invention, a kind of antithrombotic, the preparation method of medicine for the treatment of thrombosis, bag Include following steps:

Herba Gei is dried and cut into fragment；

Fragment it is immersed in the ethanol that concentration is 80% 6 hours and repeats to extract 3 times, merging and carry through ethanol The extracting solution that takes also is spray dried to pressed powder, obtains ethanol extraction；

Soluble in water for ethanol extraction and priority petroleum ether, ethyl acetate and n-butyl alcohol are extracted:

By the extract of petroleum ether, ethyl acetate and n-butanol extraction drying under reduced pressure under the conditions of 38 DEG C respectively Obtain the compound activity position of Herba Gei.

Above-mentioned preparation method, wherein, the described Herba Gei by ethyl acetate and n-butanol extraction Polyphenol compound and triterpenes chemical combination are isolated by the method for reverse column chromatography in compound activity position Thing.

The invention provides medicine of a kind of antithrombotic, treatment thrombosis and preparation method thereof, wherein Antithrombotic, the medicine for the treatment of thrombosis are the compound activity position extracted from Herba Gei；Institute State compound activity position and include polysaccharide compound, polyphenol compound, flavone compound and triterpene At least one in compounds, the medicine of the present invention is by the compound activity extracted from Herba Gei Position, and give human body by active site, it is possible to achieve the formation of pre-preventing thrombosis and treatment have been formed Thrombosis, the thrombosis that this active site has mainly been formed by inhibition thrombosis and dissolving, thus reach Prevention and the treatment various disease state that thrombosis causes in different tissues or organ.

Accompanying drawing explanation

By the detailed description non-limiting example made with reference to the following drawings of reading, the present invention and Feature, profile and advantage will become more apparent upon.The labelling taken charge of mutually in whole accompanying drawings refers to the portion taken charge of mutually Point.The most deliberately it is drawn to scale accompanying drawing, it is preferred that emphasis is the purport of the present invention is shown.

Fig. 1 a and Fig. 1 b be the present invention be whole blood thrombolysis amount time graph schematic diagram in example；

The compound of the different component that Fig. 2 a and Fig. 2 b provides for the present invention dissolving energy to whole blood blood clot Power schematic diagram；

The treatment of microthrombusis patient is made by the active site of the oral administration of compound that Fig. 3 provides for the present invention Use schematic diagram.

Detailed description of the invention

In the following description, a large amount of concrete details is given to provide to the present invention the most thoroughly Understand.It is, however, obvious to a person skilled in the art that the present invention can be without one Or multiple these details and be carried out.In other example, in order to avoid obscuring with the present invention, Technical characteristics more well known in the art are not described.

In order to thoroughly understand the present invention, detailed step and detailed knot will be proposed in following description Structure, in order to explaination technical scheme.Presently preferred embodiments of the present invention is described in detail as follows, but In addition to these describe in detail, the present invention can also have other embodiments.

Embodiment 1, as illustrated in figs. ia and ib, the whole blood thrombolysis amount time graph that the present invention provides.

From SD rat tail vein collection 1ml blood, being put in small test tube, it is placed in obliquity in 4 DEG C In refrigerator.When being not added with anticoagulant, blood there will be solidification phenomenon, and reason is the fibrin in blood plasma The former fibrin that becomes, fibrin and hemocyte together form blood clot, and upper strata is remaining is blood Clearly.Comparison, is the upper serum in test tube to be removed, adds the normal saline of the sodium chloride of 10ml 0.9%. AF-H, is the normal saline that the addition 10ml in the test tube removing serum is contained 167mg active site, And AF-L, it is the physiology salt that the addition 10ml in the test tube removing serum is contained 16.7mg active site Water.Ethanol-H, is the life that the addition 10ml in the test tube removing serum is contained 833mg ethanol extraction Reason saline, and ethanol-L, be that the addition 10ml in the test tube removing serum is contained 83.3mg ethanol extraction The normal saline of thing.Polyphenol-H, is that the addition 10ml in the test tube removing serum is contained 167mg pubescence The normal saline of Fructus Adinae total polyphenols, and polyphenol-L, be to be contained by the addition 10ml in the test tube removing serum There is the normal saline of 16.7mg Herba Gei total polyphenols.Triterpene-H, is by the test tube removing serum Add 10ml and contain the normal saline of 16.7mg Herba Gei total triterpene, and triterpene-L, it is to remove Addition 10ml in the test tube of serum contains the normal saline of 1.67mg Herba Gei total triterpene.Above Sample standard deviation is placed in 37 DEG C of water-baths.The most respectively after cultivating 1,2,4,8,24,48 hours, respectively The weight of thrombosis in each different test tubes of difference precision weighing on individual time point.That mentions in the present invention is total Triterpene is the abbreviation of triterpenoid compound, and total polyphenols is the abbreviation of polyphenol compound, active site be from The abbreviation at the compound activity position that Herba Gei extracts.

Embodiment 2, as shown in Fig. 2 a and Fig. 2 b, the compound of the different component that the present invention provides is to entirely The solvability of blood blood clot.

In the thrombosis test tube that will be formed containing 1ml blood, add the normal saline of the sodium chloride of 10ml 0.9%. AF-H, is by containing the life containing 167mg active site with addition 10ml in the test tube compareing equivalent thrombosis Reason saline, and AF-L, be that the addition 10ml in the test tube containing equivalent thrombosis contains 16.7mg activity The normal saline at position.Ethanol-H, is that the addition 10ml in the test tube containing equivalent thrombosis is contained 833mg The normal saline of ethanol extraction, and ethanol-L, be by the addition 10ml in the test tube containing equivalent thrombosis Normal saline containing 83.3mg ethanol extraction.Polyphenol-H, is by the test tube containing equivalent thrombosis Add 10ml and contain the normal saline of 167mg Herba Gei total polyphenols, and polyphenol-L, it is to contain Addition 10ml in the test tube of amount thrombosis contains the normal saline of 16.7mg Herba Gei total polyphenols.Three Terpene-H, is that the addition 10ml in the test tube containing equivalent thrombosis is contained 16.7mg Herba Gei total three The normal saline of terpene, and triterpene-L, be to be contained by the addition 10ml in the test tube containing equivalent thrombosis The normal saline of 1.67mg Herba Gei total triterpene.Sample above is placed in 37 DEG C of water-baths.Then divide Not after cultivating 24 hours, the thrombosis in each test tube is taken out, weighs its dry weight, then divided by variant The initial weight in wet base of thrombosis in test tube.

Embodiment 3, is illustrated in figure 3 the active site of the oral administration of compound that the present invention provides to microthrombus shape Become the therapeutical effect of patient.No.1-4 is the dark of 4 different microthrombusiss having in blood respectively The visual field (Bu Shi mirror) peripheral blood blood smear.Blood smear (above arranging smear) when going to a doctor at the beginning of patient, Visible microthrombusis (red round inclusion region).The also mutual coagulation adhesion of the hemocyte of patient becomes bulk Or string-like form.And with active site (1 gram every time, one day 2 times) oral medication patient after one week (in Row smear 1week), it is seen that all four patient's blood smear do not finds any microthrombus, and seriously The hemocyte of coagulation significantly takes a turn for the better.(lower row smear 2week), all four patient after treating two weeks Blood smear not only do not find any microthrombus, and the state of aggregation of hemocyte is completely recovered to normally, The form of hemocyte also recovers normal, the most well separates.

To illustrate that all method steps used in the present invention meet NIH and send out The laboratory animal protection of table and guide for use.

The following is separation Herba Gei (Geum japonicum Thunb.Var.Chinense P.Bolle) The specific embodiment of active site of compound.

A kind of antithrombotic, the preparation method of medicine for the treatment of thrombosis, comprise the following steps:

Step S1: Herba Gei is dried and cut into fragment；July in summer works as from China Guizhou Province crow County gathers Herba Gei, and is dried.

Step S2: fragment is immersed in the ethanol that concentration is 80% 6 hours and repeats to extract 3 times, closes And through the extracting solution of ethanol extraction and be spray dried to pressed powder, obtain ethanol extraction；Radix Glycyrrhizae (20 Kilogram) be soaked in 80% ethanol (120 liters) and at room temperature soak 6 hours, repeat to extract 3 times.Merge Extracting solution, is spray dried to pressed powder, obtains ethanol extraction.

Step S3: soluble in water for ethanol extraction and priority petroleum ether, ethyl acetate and n-butyl alcohol are carried out Extraction；Specifically, the pressed powder of this ethanol extraction to be dissolved in 25 literss of water, successively with petroleum ether (20 L), ethyl acetate (20L), then the extraction of n-butyl alcohol (20L) three subzone, draws three parts.

Step S4: by the extract of petroleum ether, ethyl acetate and n-butanol extraction respectively under the conditions of 38 DEG C Drying under reduced pressure obtains the compound activity position of Herba Gei.By big at SD respectively for three kinds of powder drawing Mus has carried out toxotest.Found that except petroleum ether portion, laboratory animal is had certain poison Outside property, it is that 15 gs/kg of body weight do not find any visible toxicity yet that other 2 kinds of extracts use dosage. In test tube, thrombus dissolving experiment shows that n-butanol extraction composition (mainly contains polysaccharide, Polyphenols, flavonoid And triterpenoid compound) thrombolytic effect preferably (Fig. 1), so using this extraction components as AF. By the method for reverse column chromatography, from the component of AF and ethyl acetate, isolate total polyphenols and total triterpene respectively.

The most just according to the component of extraction and the thrombolytic efficiency extracting each component separated is contrasted.

Embodiment 4, extracts, from Herba Gei, the thrombolytic efficiency of each component separated in order to measure, point Not from SD rat (300 grams, often 6 rats of group) tail venous collection 1ml blood, it is put in small test tube respectively In, and these test tubes are positioned over obliquity in 4 DEG C of refrigerators.Owing to not adding anticoagulant in test tube, Fibrinogen in blood plasma becomes fibrin, and fibrin and hemocyte together form blood clot, Upper strata is remaining is serum.30 test tubes containing blood clot are divided into 5 groups.

1) process of matched group is the upper serum in test tube to be removed, and the original weight in wet base of weighing thrombosis adds Enter the normal saline of the sodium chloride of 10ml 0.9%；

2) AF high dose process group is to remove serum, weighing wet weight of thrombus, and the addition 10ml in test tube Normal saline containing 120mg AF；

3) AF low dosage process group is to remove serum, weighing wet weight of thrombus, and the addition 10ml in test tube Normal saline containing 12mg AF；

4) ethanol extraction high dose process group is to remove the serum in test tube, the weight in wet base of weighing thrombosis, so After in test tube, add 10ml contain the normal saline of 600mg ethanol extraction；

5) ethanol extraction low dosage process group is to remove the serum in test tube, the weight in wet base of weighing thrombosis, so After in test tube, add 10ml contain the normal saline of 60mg ethanol extraction；

6) total polyphenols high dose process group is to remove the serum in test tube, the weight in wet base of weighing thrombosis, then exists Test tube adds the normal saline that 10ml contains 140mg Herba Gei total polyphenols；

7) total polyphenols low dosage process group is to remove the serum in test tube, the weight in wet base of weighing thrombosis, then exists Test tube adds the normal saline that 10ml contains 14mg Herba Gei total polyphenols；

8) total triterpene high dose process group is to remove the serum in test tube, the weight in wet base of weighing thrombosis, then exists Test tube adds the normal saline that 10ml contains 20mg Herba Gei total triterpene；

9) total triterpene low dosage process group is to remove the serum in test tube, the weight in wet base of weighing thrombosis, then exists Test tube adds the normal saline that 10ml contains 2mg Herba Gei total triterpene.Sample above is placed in In 37 DEG C of water-baths.The most respectively after cultivating 1,2,4,8,24,48 hours, on each time point The weight of thrombosis in each different test tubes of precision weighing respectively.

Another group experiment is the compound the measuring different component solvability to whole blood blood clot.It is equally The sample of above-mentioned 9 groups of different disposal, sample above is placed in 37 DEG C of water-baths.Cultivating 24 the most respectively After hour, the undissolved thrombosis in each test tube is taken out, weighs its dry weight, then divided by variant examination The initial weight in wet base of thrombosis in pipe.Test result indicate that as it is shown in figure 1, either high dose or low dosage Processing thrombosis, the thrombolytic ability of AF process group is the strongest, is significantly higher than other all groups.At ethanol extraction Reason group also has certain thrombolytic ability, but is below AF process group.Although the thrombolytic energy of total triterpene process group Power has preferably rising after cultivating 8 hours, and total polyphenols does not the most find significant thrombolytic ability.Just For the thrombolytic ability of each group of process medicine of experiment prompting, the high or AF of low dosage has Strong thrombus dissolving ability；Ethanol extraction also has preferable thrombolytic ability, but thrombolytic ability is less than at AF Reason group, prompting AF is thrombolytic main active site.The total triterpene of high dose is also demonstrated by necessarily Thrombolytic ability as shown in Figure 2.

One is provided the most in fact to the therapeutical effect suffering from microthrombus and left ventricular wall thrombosis clinically below with regard to AF Execute example.

Embodiment 5

Result based on the experiment of above-mentioned Lab dissolution thrombosis, it is often more important that Herba Gei is accredited as It is an edible plants, and one of is the 8 big wild herbs of Guizhou Province local resident, and in our experiment Confirm the character of its totally nontoxic (20 gs/kg of body weight), the situation of written consent in the know patient On the basis of lower and human treatment, clinical practice tests its AF bigger to microthrombus and left ventricular wall The therapeutic effect of thrombosis patients.Totally four have finger tip pain, headache, dizzy, and capillary rupture is high The patient of blood pressure, the hemocyte in Bu Shi mirror finger tip peripheral blood plate coating checking finds blood is aggregated into mutually Bulk or string-like form, and it is found to have obvious microthrombusis (Fig. 3).These four patients received oralization The active site (1 gram every time, one day 2 times) of compound.Take institute's check later in a week.Patient's readme refers to End pain and headache and dizzy symptom substantially alleviate, and peripheral blood Bu Shi spectroscopy finds, all four patient's blood Smear does not finds any microthrombus, and the hemocyte of the serious agglomerating and irregular string-like of coagulation is the most notable Take a turn for the better (Fig. 3).After treating two weeks, check finds, the finger tip pain of patient, headache, dizziness are complete Disappearing, blood pressure is also decreased obviously.Peripheral blood Bu Shi spectroscopy finds, the blood smear of all four patient is not Only do not find any microthrombus, and the state of aggregation of hemocyte is also completely recovered to normally, hemocyte Form is also recovered normally (Fig. 3).The patient of another heart Anterior wall myocardial infarction of 65 years old, the ultrasonic heart Cardon inspection finds that there is a diameter about 1.5cm thrombosis at anterior infarcts position, left room.This patient is administered orally chemical combination The active site (1 gram every time, one day 2 times) of thing.After taking one week, ultrasoundcardiogram check finds thrombosis Substantially diminishing, significantly diminish after taking two weeks, after taking three weeks, the thrombosis of left locular wall is wholly absent. In the present invention, a kind of antithrombotic, the medicine for the treatment of thrombosis, individual from soft by being orally administered to The compound activity position that hair Fructus Adinae extracts is come antithrombotic or treatment thrombosis and can pass through skin Hemostasis, intramuscular injection or venoclysis give the individual compound activity position extracted from Herba Gei Coming antithrombotic or treatment thrombosis, wherein, the amount given is 0.1mg-8g compound activity position / kilogram whose body weight/sky, antithrombotic, treatment thrombosis include tissue or organ tremulous pulse, vein, In tissue that in blood capillary, in thrombosis, blood, thrombosis causes or organ ischemia or blood supply Disconnected.

In sum, the invention provides a kind of antithrombotic, the medicine for the treatment of thrombosis and preparation thereof Method, wherein antithrombotic, the medicine for the treatment of thrombosis are that the compound extracted from Herba Gei is lived Property position；Described compound activity position includes polysaccharide compound, polyphenol compound, flavonoid At least one in compound and triterpenoid compound, the medicine of the present invention is by from Herba Gei extraction Compound activity position, and give human body by active site, it is possible to achieve the formation of pre-preventing thrombosis and treatment The thrombosis formed, the thrombosis that this active site has mainly been formed by inhibition thrombosis and dissolving, Thus reach prevention and the treatment various disease state that thrombosis causes in different tissues or organ.

Above presently preferred embodiments of the present invention is described.It is to be appreciated that the invention is not limited in Above-mentioned particular implementation, the equipment not described in detail the most to the greatest extent and structure are construed as with in this area Common mode be practiced；Any those of ordinary skill in the art, without departing from the technology of the present invention side Under case ambit, technical solution of the present invention is made by the method and the technology contents that all may utilize the disclosure above Many possible variations and modification, or it is revised as the Equivalent embodiments of equivalent variations, this has no effect on this Bright flesh and blood.Therefore, every content without departing from technical solution of the present invention, according to the skill of the present invention Art essence, to any simple modification made for any of the above embodiments, equivalent variations and modification, all still falls within this In the range of the protection of bright technical scheme.

Claims

1. an antithrombotic, the medicine for the treatment of thrombosis, it is characterised in that described medicine is from soft The compound activity position that hair Fructus Adinae extracts；Described compound activity position include polysaccharide compound, At least one in polyphenol compound, flavone compound and triterpenoid compound.

A kind of antithrombotic the most as claimed in claim 1, the medicine for the treatment of thrombosis, its feature exists In, described compound activity position includes at least one in polyphenol compound, triterpenoid compound.

A kind of antithrombotic the most as claimed in claim 1, the medicine for the treatment of thrombosis, its feature exists In, carry out antithrombotic by being orally administered to the individual compound activity position extracted from Herba Gei Or treatment thrombosis.

A kind of antithrombotic the most as claimed in claim 1, the medicine for the treatment of thrombosis, its feature exists In, give the individual chemical combination extracted from Herba Gei by subcutaneous injection, intramuscular injection or venoclysis Thing active site comes antithrombotic or treatment thrombosis.

A kind of antithrombotic the most as claimed in claim 4, the medicine for the treatment of thrombosis, its feature exists In, described in the amount that gives be 0.1mg-8g compound activity position/kilogram whose body weight/sky.

6. a kind of antithrombotic as described in claim 1-5 is arbitrary, the medicine for the treatment of thrombosis, its Being characterised by, described antithrombotic, treatment thrombosis include tissue or organ tremulous pulse, vein, capillary Intravascular Thrombus is formed, thrombosis causes in blood tissue or organ ischemia or blood supply interrupt.

7. the preparation method of the medicine of an antithrombotic, treatment thrombosis, it is characterised in that include Following steps include:

Herba Gei is dried and cut into fragment；

A kind of antithrombotic the most as claimed in claim 3, the preparation method of medicine for the treatment of thrombosis, It is characterized in that, described by the compound activity position of ethyl acetate and the Herba Gei of n-butanol extraction Polyphenol compound and triterpenoid compound is isolated by the method for reverse column chromatography.