CN105900707A - Screening method for saline and alkaline resistance of kok-saghyz - Google Patents
Screening method for saline and alkaline resistance of kok-saghyz Download PDFInfo
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- CN105900707A CN105900707A CN201610268087.XA CN201610268087A CN105900707A CN 105900707 A CN105900707 A CN 105900707A CN 201610268087 A CN201610268087 A CN 201610268087A CN 105900707 A CN105900707 A CN 105900707A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention relates to the field of plant physiology, in particular to a screening method for the saline and alkaline resistance of kok-saghyz. The screening method for the saline and alkaline resistance of the kok-saghyz is provided for the problem that the kok-saghyz cannot be massively planted in a saline and alkaline land, and the resistance degree of the kok-saghyz to saline and alkaline is visually and easily judged by determining the physiological and biochemical indexes of the kok-saghyz on the condition of simulating saline-alkaline stress through soda water with the different concentrations. Through the method, on one hand, existing saline-alkaline-resistant kok-saghyz can be identified, and on the other hand, the saline and alkaline resistance of inbred lines or hybrid species of the kok-saghyz can be evaluated and screened; looking for the high saline-alkaline-resistant kok-saghyz germplasm resources is the key development direction of the saline and alkaline resistance of the kok-saghyz, and a foundation is laid for creation of the good germplasm resources.
Description
Technical field
The present invention relates to plant physiology field, be specifically related to a kind of russian dandelion and resist saline and alkaline screening method.
Background technology
Natural rubber and iron and steel, oil, coal the referred to as four big raw materials of industry, the natural rubber 90% that the present world is main
It is above the Para rubber tree of growth under the torrid zone, subtropical environment.China's import of natural rubber from calendar year 2001 is super
Crossing the U.S. and become the first in the world importer, self-produced rubber is far from meeting domestic demand, and the geographical position of China determines
Only Hainan, Yunnan and the wide Three Eastern Provinces can be with Planting of Brazil rubber tree, and cultivated area is limited.So, find exploitation new
Available glue resource, oneself is through extremely urgent.
Russian dandelion (Taraxacum kok-saghyz) is Compositae Dandelion perennial root herbaceous plant.Rubber grass roots
Being contained within rubber, latex performance slightly inferior to the latex of Para rubber tree, can obtain a large amount of second while carrying out extracting rubber
Alcohol.
Owing to russian dandelion is the lactiferous plant of draft, growth cycle is short, it is easy to genetic transformation, and the latex dust of russian dandelion also can be made
Identifying with carrying out product glue functional gene for natural plants reactor, therefore, the growth conditions of research russian dandelion, industrialization must be planted
Russian dandelion not only has the highest economic worth in commercial Application, and has far-reaching in Plant Biotechnology research field
Scientific meaning.
Russian dandelion originates in mountain valley, the republic of Kazakhstan Tianshan Mountains and Xinjiang, China Tekes River basin, ground, the Soviet Union Central Asia
Also there is distribution in district and Europe.China's land resource is in short supply, develops russian dandelion only with marginal land, seriously constrains russian dandelion
Industrialization, the land in Heilongjiang Province gross area 47.3 ten thousand hm2, account for the 4.9% of the whole nation soil gross area, before possessing scale plantation
Putting forward condition, but the land resource that Heilongjiang Province's Area comparison is big at present is salt-soda soil, western song-Nen plain is that the world three is big
One of Soda saline and alkaline soils areal concentration, the nearlyest 66.7 ten thousand hm2 of saline-alkali soil area, the wherein salinity solonchak more than 1% and saline and alkaline
Soil is more than 100,000 hm2, and soil is based on alkali meadow soil, and pH value is generally higher than more than 8.Investigate according to the nineties in 20th century, the whole province
Salinization soil adds 13.3 ten thousand hm2 than the fifties, and year increases hm2 more than 2600.The continuous expansion in salt-soda soil seriously destroys
The ecological environment of locality, be also the subject matter of long-standing problem agriculture-stock production.The large-area salt-soda soil of our province does not obtain
Well develop, therefore, the research russian dandelion condition of planting in salt-soda soil become russian dandelion spread important before
Carry.
This research is intended providing a kind of russian dandelion to resist saline and alkaline screening method, by measuring under Saline-Sodic Stress, and russian dandelion
The data such as cell leakage, deeper into understanding the impact that russian dandelion grow by Saline Alkali Stress, mensuration index of correlation obtains rubber
Grass physiological compensatory mechanism under Saline Alkali Stress, introduces a fine variety for it and carries out implantation in large scale and realize industrialization based theoretical.
Summary of the invention
Can not be in the problem of salt-soda soil implantation in large scale for russian dandelion, the goal of the invention of the present invention is: by measuring
With the physiological and biochemical index of russian dandelion under the conditions of variable concentrations soda water analog salt alkaline stress, intuitively simply judge this russian dandelion
To saline and alkaline resistance level.Existing anti-saline and alkaline russian dandelion is identified, separately by the most available technical solution of the present invention
On the one hand the Saline alkali tolerance of russian dandelion selfing line or cenospecies can be evaluated screening, find the rubber grass seeds matter of Salt And Alkali Tolerance
Resource, is the prior development direction of russian dandelion Saline alkali tolerance from now on, lays the foundation for creating excellent germ plasm resource.
The present invention solves the technological means of the problems referred to above, it is provided that a kind of russian dandelion resists saline and alkaline screening method, including with
Lower step:
A, with after the soil incubation paramount 4-5cm of russian dandelion that pH value is 6.5-7, be randomly divided into 6 groups, carry out Saline Alkali Stress test；
B, irrigating Simulated Soil Saline Alkali Stress with the soda water that concentration is different respectively, the soda water concentration of each group is respectively as follows:
0.0wt%, 0.2wt%, 0.4wt%, 0.6wt%, 0.8wt%, 1.0wt%, water a soda water for every 1-2 days, measures rubber after 10-15 days
At least one in blade cell membrane permeability, chlorophyll content or the mda content of grindelia；
C, after step B terminates, often group russian dandelion all with the soda water of 0.0wt%, soil is irrigated, within every 1-2 days, water a soda
Water, every day, timing measured in the soluble sugar content of russian dandelion, soluble protein content, proline content or beet alkali content
At least one；
D, Analysis and Screening go out russian dandelion saline-alkali tolerance and physiological compensatory.
Wherein, the method for Analysis and Screening described in step D is as follows:
When step B detects permeability of cell membrane, owing to cell membrane is the most sensitive position that cell experiences that external environment is coerced,
Various adverse circumstances injure, and first act on cell membrane, cause cell membrane penetration to sexually revise or lose, cause intracellular thing
Matter exosmoses in a large number, degree that its direct reaction cell is hurt and resistance, organizes 1 in step B for compareing, according to cell membrane
Permeability judges the saline-alkali tolerance of russian dandelion, and permeability is the highest, and saline-alkali tolerance is the poorest；
When step B detects chlorophyll content, owing to chlorophyll is main photosynthesis raw material, also can well reflect salt
The upgrowth situation of russian dandelion under the conditions of alkaline stress, in step B, group 1 is comparison, judges the anti-of russian dandelion according to chlorophyll content
Saline alkali, chlorophyll content is the highest, and saline-alkali tolerance is the best；
When detecting mda content in step B, owing to malonaldehyde is the end-product of membrane lipid peroxidatio, its content height is directly
Reflecting the order of severity of cell Saline Alkali Stress, its main injury is to cause membrane lipid peroxidatio, damage biological membrane structure, main
If cytoplasma membrane so that membrane structure and functionally sustaining damage, change the permeability of film, thus affect a series of physiology
Being normally carried out of biochemical reaction, organizes 1 for compareing in step B, judges the saline-alkali tolerance of russian dandelion according to mda content, and the third two
Aldehyde is the highest, and saline-alkali tolerance is the poorest；
When detecting soluble sugar and soluble protein content in step C, soluble sugar and soluble protein are considered as plant
The little molecule solute of induction under Saline Alkali Stress, they can regulate Osmotic balance, participate in the physiology after russian dandelion Saline Alkali Stress and mend
Repay；In step C, group 1 is comparison, judges the physiological compensatory function of russian dandelion according to soluble sugar and soluble protein content, can
Dissolubility sugar and soluble protein content are the highest, and russian dandelion physiological compensatory function is the strongest；
When step C detecting proline or during beet alkali content, under adverse environmental factor, degeneration-resistant to plant of proline and glycine betaine
Property plays very important effect；Proline has water retention, can reduce the cell flow of water, the extraneous osmotic stress of opposing, has
It is beneficial to Genes For Plant Tolerance saline and alkaline；Glycine betaine is the same with proline, is a kind of osmotic adjustment, and glycine betaine has maintenance cell with extraneous
The Osmotic balance of environment, the 26S Proteasome Structure and Function of stabilate macromole, release the effect that enzymatic activity is injured by high salt concentration；Saline and alkaline
Under the conditions of, proline and beet alkali content increase, and in step C, group 1 is comparison, judges rubber according to proline or beet alkali content
The physiological compensatory function of grindelia, proline or beet alkali content are the highest, and russian dandelion physiological compensatory function is the strongest.
Wherein, above-mentioned russian dandelion resists in saline and alkaline screening method, and the random packet described in step A is more than 6 groups.
Wherein, above-mentioned russian dandelion resists in saline and alkaline screening method, and soda water described in step B is aqueous sodium carbonate, and it is also
Can replace by NaCl solution.
Wherein, above-mentioned russian dandelion resists in saline and alkaline screening method, and the assay method of each physiological parameter is biologically normal
Rule detection method.
The invention have the benefit that present invention soda water analog salt alkaline stress carries out resistance screening, energy to russian dandelion
Effecting reaction russian dandelion upgrowth situation under the conditions of different saline and alkalines, filters out the maximum salt base number of suitable rubbers grass growth,
Lay a good foundation introducing a fine variety of saline and alkaline area for russian dandelion, have important practical significance and economic benefit.
Detailed description of the invention
Below in conjunction with embodiment, technical solution of the present invention is elaborated, but be not only and invention will be limited to embodiment
In the range of described.
Embodiment
A, with after the soil incubation paramount 4-5cm of russian dandelion that pH value is 6.5-7, randomly choose wherein 120 strain, be divided into 6 groups,
Numbered a, b, c, d, e, f group carries out Saline Alkali Stress test；
B, irrigating Simulated Soil Saline Alkali Stress with the soda water that concentration is different respectively, the soda water concentration of each group is respectively as follows: a group
0.0wt%, b group 0.2wt%, c group 0.4wt%, d group 0.6wt%, e group 0.8wt%, f group 1.0wt%, within every 2 days, water a soda water, water
During water, soil is irrigated, measure respectively after 14 days and often organize blade cell membrane permeability, chlorophyll content and the malonaldehyde of russian dandelion and contain
Amount；
C, after step B terminates, often group russian dandelion all with the soda water of 0.0wt%, soil is irrigated, within every 2 days, water a soda water,
Every day 12 noon measure the soluble sugar content of russian dandelion, soluble protein content, proline content and beet alkali content contain
Amount；
D, Analysis and Screening go out russian dandelion saline-alkali tolerance and physiological compensatory.
With the permeability of cell membrane in russian dandelion blade in conductance method determination step B, measure russian dandelion with acetone extraction
Blade Determination of Chlorophyll content, measure mda content in russian dandelion blade, with in phynol method determination step C with return law of the straight line
Soluble sugar content in russian dandelion blade, measure soluble protein content in russian dandelion blade by LoWry method, use acid ninhydrine
Method measures proline content in russian dandelion blade, uses beet alkali content in Rubber by high Performance Liquid Chromatography blade of grass sheet, result
As shown in table 1 below:
The physical signs of russian dandelion blade under the conditions of the different Saline Alkali Stress of table 1
As can be seen from the above table: found by significance analysis, each physical signs a, b, c group difference is not the most notable, is in putting down
The platform phase, just beginning with significant difference during to d group, russian dandelion is well-grown, the degree suffered damage in the saline and alkaline condition of a-c group
It is the least, until under the Saline Alkali Stress of d group concentration, russian dandelion blade starts damaged seriously, and the salt tolerant basicity of russian dandelion is concentration
The soda water concentration of 0.4wt%.
Claims (5)
1. a russian dandelion resists saline and alkaline screening method, it is characterised in that comprise the following steps:
A, with after the soil incubation paramount 4-5cm of russian dandelion that pH value is 6.5-7, be randomly divided into 6 groups, carry out Saline Alkali Stress test；
B, irrigating Simulated Soil Saline Alkali Stress with the soda water that concentration is different respectively, the soda water concentration of each group is respectively as follows:
0.0wt%, 0.2wt%, 0.4wt%, 0.6wt%, 0.8wt%, 1.0wt%, water a soda water for every 1-2 days, measures after 10-15 days
At least one in blade cell membrane permeability, chlorophyll content or the mda content of russian dandelion；
C, after step B terminates, often group russian dandelion all with the soda water of 0.0wt%, soil is irrigated, within every 1-2 days, water a soda
Water, every day, timing measured in the soluble sugar content of russian dandelion, soluble protein content, proline content or beet alkali content
At least one；
D, Analysis and Screening go out russian dandelion saline-alkali tolerance and physiological compensatory.
Russian dandelion the most according to claim 1 resists saline and alkaline screening method, it is characterised in that: Analysis and Screening described in step D
Method as follows:
When detecting permeability of cell membrane in step B, in step B, group 1 is comparison, judges russian dandelion according to permeability of cell membrane
Saline-alkali tolerance, permeability is the highest, and saline-alkali tolerance is the poorest；
When detecting chlorophyll content in step B, in step B, group 1 is comparison, judges the anti-of russian dandelion according to chlorophyll content
Saline alkali, chlorophyll content is the highest, and saline-alkali tolerance is the best；
When detecting mda content in step B, in step B, group 1 is comparison, judges the anti-of russian dandelion according to mda content
Saline alkali, mda content is the highest, and saline-alkali tolerance is the poorest；
When step C detecting soluble sugar and during soluble protein content, organize 1 for comparison in step C, according to soluble sugar with
Soluble protein content judges the physiological compensatory function of russian dandelion, and soluble sugar and soluble protein content are the highest, and russian dandelion is raw
It is the strongest that reason compensates function；
When detecting proline or beet alkali content in step C, in step C, group 1 is comparison, contains according to proline or glycine betaine
Amount judges the physiological compensatory function of russian dandelion, and proline or beet alkali content are the highest, and russian dandelion physiological compensatory function is the strongest.
Russian dandelion the most according to claim 1 resists saline and alkaline screening method, it is characterised in that: the random packet described in step A
It it is more than 6 groups.
Russian dandelion the most according to claim 1 resists saline and alkaline screening method, it is characterised in that: soda water described in step B is carbon
Acid sodium aqueous solution, it can also be replaced by NaCl solution.
Russian dandelion the most according to claim 1 resists saline and alkaline screening method, it is characterised in that: the assay method of each physiological parameter
For common detection methods biologically.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610268087.XA CN105900707A (en) | 2016-04-27 | 2016-04-27 | Screening method for saline and alkaline resistance of kok-saghyz |
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| CN201610268087.XA CN105900707A (en) | 2016-04-27 | 2016-04-27 | Screening method for saline and alkaline resistance of kok-saghyz |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7540438B2 (en) * | 2006-05-23 | 2009-06-02 | Buranov Anvar U | Process for recovering rubber from rubber-bearing plants with a gristmill |
| CN101803516A (en) * | 2010-03-29 | 2010-08-18 | 天津师范大学 | Method for improving salt resistance of lawn plant by filling soil through adopting waste colloidal particle as salt storing layer |
| CN103004610A (en) * | 2013-01-10 | 2013-04-03 | 王昱蓉 | Method for high-salt screening of saline-alkali resistant rosa chinensis plant |
-
2016
- 2016-04-27 CN CN201610268087.XA patent/CN105900707A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7540438B2 (en) * | 2006-05-23 | 2009-06-02 | Buranov Anvar U | Process for recovering rubber from rubber-bearing plants with a gristmill |
| CN101803516A (en) * | 2010-03-29 | 2010-08-18 | 天津师范大学 | Method for improving salt resistance of lawn plant by filling soil through adopting waste colloidal particle as salt storing layer |
| CN103004610A (en) * | 2013-01-10 | 2013-04-03 | 王昱蓉 | Method for high-salt screening of saline-alkali resistant rosa chinensis plant |
Non-Patent Citations (2)
| Title |
|---|
| 刘祖祺和张石城: "《植物抗性生理》", 31 December 1994 * |
| 王柳: "地被植物连钱草、蛇莓盐碱胁迫研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
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Application publication date: 20160831 |
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