CN105876507A - Compound biological enzyme degraded melon seeds and processing method thereof - Google Patents
Compound biological enzyme degraded melon seeds and processing method thereof Download PDFInfo
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- CN105876507A CN105876507A CN201510034924.8A CN201510034924A CN105876507A CN 105876507 A CN105876507 A CN 105876507A CN 201510034924 A CN201510034924 A CN 201510034924A CN 105876507 A CN105876507 A CN 105876507A
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- enzyme
- semen benincasae
- melon seeds
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Abstract
The present invention discloses compound biological enzyme degraded melon seeds and a processing method thereof. Fresh water, compound biological enzymes and glucose are added into the dried melon seeds with shells to conduct degradation, and then the degraded melon seeds are prepared by drying. The compound biological enzymes consist of cellulase, hemicellulase, pectinase, neutral protease, lipase and phosphoester hydrolase. The processing steps of the degraded melon seeds are as follows: the fresh melon seeds are washed clean, the washed melon seeds are dried until the moisture content is below 10%, and the dried melon seeds with shells are prepared; the compound biological enzymes and glucose are added into fresh water to be stirred evenly; the melon seeds with shells are added into the mixture in a step B to be stirred evenly; the mixture is placed at a temperature of 15-60 DEG C in the dark to conduct heat preservation and anaerobic degradation for 4-18 hours; the anaerobic degraded mixture is taken out and the taken out anaerobic degraded mixture is dried at a temperature of 50-75 DEG C to a moisture content below 10%; and the dried mixture is sealed and packaged. The processing method can significantly improve the content of protein small peptides in the melon seeds, eliminate anti-nutritional factors, reduce the fat content, reduce toxins, prolong the shelf life of the melon seeds, and provide a whole new way for the development of new products of the melon seeds.
Description
Technical field
The present invention relates to Semen Benincasae processing technique field, especially a kind of compound bio enzymatic degradation Semen Benincasae and processing method thereof.
Background technology
Semen Benincasae have the kinds such as Semen Helianthi, Semen Benincasae, Semen Citrulli, Semen Cucurbitae, Semen Cucumidis sativi, Semen Luffae.Except processing vegetable oil, leisure food also can be processed into.Owing to Semen Benincasae belong to higher fatty acid, high-protein food, often eat, easily cause dyspepsia and human body is got fat.The long-term Semen Benincasae preserved, easily occur Oxidation of Fat and Oils to go bad, and the moisture absorption also can produce substantial amounts of carcinogen----flavacin after going mouldy, and health is stayed potential safety hazard.
Utilize modern biotechnology enzymatic degradation technology, the antinutritional factor such as the urase in Semen Benincasae albumen, pancreas neutral protein enzyme inhibition factor, haemoglutinin, Semen Benincasae globulin, β-companion's globulin, phytic acid can be eliminated to greatest extent, effectively the protein of macromole is converted into the nonantigenic high-quality little peptide protein source of small molecular protein and little peptide, and the materials such as substantial amounts of oligopeptide, glutamic acid, lactic acid, vitamin, UGF can be produced.The lactic acid fragrance simultaneously producing uniqueness can increase appetite.But the most directly dry for band shell Semen Benincasae are not carried out the report of bio-enzyme degradation.
Summary of the invention
It is an object of the invention to provide and a kind of dry for band shell Semen Benincasae directly carry out bio-enzyme degradation, significantly improve Semen Benincasae protein small peptide content, eliminate antinutritional factor, reduce fat content, reduce toxin, the compound bio enzymatic degradation Semen Benincasae extending the shelf-life of Semen Benincasae and processing method thereof, the exploitation for Semen Benincasae new product provides a kind of brand-new approach.
The technical scheme is that compound bio enzymatic degradation Semen Benincasae and processing method thereof, degrade by adding clear water, compound biological enzyme and glucose in the dry Semen Benincasae of band shell, be then dried and prepare degraded Semen Benincasae.
Described compound biological enzyme is made up of cellulase, hemicellulase, pectase, neutral protease, lipase, phosphate fire-resistant oil enzyme and lysozyme.
Described degraded Semen Benincasae procedure of processing is:
A. by fresh Semen Benincasae cleaning, drying to moisture content less than 10%, the dry Semen Benincasae of band shell are prepared；
B. compound biological enzyme and glucose are added in clear water and stir；
C. dry for band shell Semen Benincasae are added in step B and stir；
D. lucifuge, insulation and anaerobic degradation 4-18 hour under the conditions of 15-60 ° of C；
E. take out, be dried under the conditions of 50-75 ° of C to moisture content less than 10%；
F. pack.
The described dry Semen Benincasae of band shell with compound biological enzyme, glucose formula proportion are by weight:
The dry Semen Benincasae of band shell
1000 parts,
Clear water
100-800 part,
Cellulase
0.2-0.5 part,
Hemicellulase
0.2-0.5 part,
Pectase
0.1-0.4 part,
Neutral protease
0.3-0.6 part,
Lipase
0.3-0.6 part,
Phosphate fire-resistant oil enzyme
0.1-0.2 part,
Lysozyme
0.1-0.3 part,
Glucose
50 parts.
Described cellulose enzyme activity 150000u/g, hemicellulase enzyme 150000u/g alive, pectase enzyme 100000u/g alive, neutral protease enzyme 100000u/g alive, lipase activity 50000u/g, phosphate fire-resistant oil enzyme enzyme is lived 50000u/g, and lysozyme enzyme is lived 50000u/g, is and commercially available meets the qualified products that food hygiene requires.
Cellulase is a kind of compound enzyme, is mainly made up of circumscribed 1,4 beta-glucanase, Endo-β-glucanase and beta-glucosidase etc., also has the xylanase of the highest vigor.Insoluble fibrin and the product being derived from cellulose can be changed into monosaccharide and the oligosaccharide of solubility.In the formula of the present invention, it is fully used by the crude fibre in the enzymatic catalysis of cellulase, melon seed cases and Semen Benincasae, both can be that other enzyme provides energy matter, accelerated degradation process, the nutritional labeling of Semen Benincasae can be increased again.Meanwhile, the cellulase being retained in Semen Benincasae can also make Semen Benincasae exempt from the infringement of mycete, extends shelf life of products.
Hemicellulase is the general name that can decompose the various enzymes constituting plant cell membrane, polysaccharide in addition to cellulose and pectin substance in aqueous.β-Isosorbide-5-Nitrae in polysaccharide-glucosan key hydrolysis can be formed p dextrin.In the formula of the present invention, by the enzymatic catalysis of hemicellulase, improve the human body digestibility to Semen Benincasae, it is to avoid dyspepsia.
Pectase is the enzyme of the main component pectic substance decomposing plant.Mainly generate after hydrolyzed pectin and be prone to by the beta galactose aldehydic acid of human consumption.Owing to melon seed cases and Semen Benincasae containing substantial amounts of pectic substance, easily cause dyspepsia.In the formula of the present invention, by the enzymatic catalysis of pectase, improve the human body digestibility to Semen Benincasae, it is to avoid dyspepsia.
Neutral protease is by restriction endonuclease a kind of obtained by the fermented extraction of bacillus subtilis, can be used for various proteolysis and processes.Utilizing the enzymatic reaction of neutral protease, vegeto-animal macro-molecular protein can be hydrolyzed into small-molecular peptides or aminoacid, be beneficial to effectively absorbing and utilizing of protein, its hydrolyzed solution AN% is high, and degree of hydrolysis is high, and local flavor is good.In the formula of the present invention, Semen Benincasae separation albumen can be hydrolyzed and become small-molecular peptides, significantly improve Semen Benincasae protein small peptide content, be greatly improved the biological value of Semen Benincasae separation albumen so that it is be prone to digested.
Lipase is carboxylic ester hydrolase class, and triglyceride hydrolysis can become unsaturated fatty acid, diglyceride, monoglyceride and glycerol.Triglyceride is the lipid that people's in-vivo content is most, and the triglyceride of excess can cause body fat.In the formula of the present invention, owing to Semen Benincasae contain substantial amounts of triglyceride, by the enzymatic catalysis of lipase, triglyceride is changed into unsaturated fatty acid, diglyceride, monoglyceride and glycerol that human body is easy and fast to absorb, body fat can be prevented, be effectively improved energy metabolism level.
Phosphate fire-resistant oil enzyme be can catalyzing hydrolysis by phosphoric acid as the enzyme of ester bond of the ester of the part of acid.In the formula of the present invention, by the enzymatic catalysis of phosphate fire-resistant oil enzyme, the key such as P-S, P-0, P-N in organophosphorus pesticide is interrupted, and generates simple inorganic compound, thus the residual of organophosphorus pesticide in melon seed cases and Semen Benincasae is greatly reduced.
Lysozyme is a kind of to hydrolyze the alkaline enzyme of mucopolysaccharide in pathogenic bacterium.Mainly by destroying the β-Isosorbide-5-Nitrae glycosidic bond between-acetylmuramic acid and the NAG in cell wall, make the insoluble mucopolysaccharide of cell wall resolve into solubility glycopeptide, cause the effusion of cell wall rupture, content to make bacterolysis.Lysozyme also can with electronegative virus protein directly in conjunction with, with DNA, RNA, apoprotein formed double salt, make virally inactivated.Therefore, there is the effects such as antibacterial, antiinflammatory, antiviral, food industry can be used as preservative.In the formula of the present invention, by the enzymatic catalysis of lysozyme, the mycete infringement to Semen Benincasae can be reduced, extend shelf life of products, reduce the potential safety hazard that health is stayed by flavacin.
Glucose in the present invention can provide sufficient energy guarantee for enzyme, it is ensured that degraded quickly starts at the degraded initial stage.
Dry for band shell Semen Benincasae are directly degraded by the formula and the technique that use the present invention, can significantly improve Semen Benincasae protein small peptide content, eliminate antinutritional factor, reduce fat content, reducing toxin, extend the shelf-life of Semen Benincasae, the exploitation for Semen Benincasae new product provides a kind of brand-new approach.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further, and wherein said raw material number, in addition to specified otherwise, is parts by weight.
Compound bio enzymatic degradation Semen Benincasae and processing method thereof, degrade by adding clear water, compound biological enzyme and glucose in the dry Semen Benincasae of band shell, is then dried and prepares degraded Semen Benincasae.
Compound biological enzyme is made up of cellulase, hemicellulase, pectase, neutral protease, lipase and phosphate fire-resistant oil enzyme.
Degraded Semen Benincasae procedure of processing is:
A. by fresh Semen Benincasae cleaning, drying to moisture content less than 10%, the dry Semen Benincasae of band shell are prepared；
B. compound biological enzyme and glucose are added in clear water and stir；
C. dry for band shell Semen Benincasae are added in step B and stir；
D. lucifuge, insulation and anaerobic degradation 5 hours under the conditions of 40-50 ° of C；
E. take out, be dried under the conditions of 50-75 ° of C to moisture content less than 10%；
F. pack.
The dry Semen Benincasae of band shell with compound biological enzyme, glucose formula proportion are by weight:
The dry Semen Benincasae of band shell
1000kg,
Clear water
250kg,
Cellulase
0.35kg,
Hemicellulase
0.35kg,
Pectase
0.25kg,
Neutral protease
0.45kg,
Lipase
0.45kg,
Phosphate fire-resistant oil enzyme
0.15kg,
Lysozyme
0.15kg,
Glucose
50kg。
Cellulose enzyme activity 150000u/g, hemicellulase enzyme 150000u/g alive, pectase enzyme 100000u/g alive, neutral protease enzyme 100000u/g alive, lipase activity 50000u/g, phosphate fire-resistant oil enzyme enzyme is lived 50000u/g, and lysozyme enzyme is lived 50000u/g, is and commercially available meets the qualified products that food hygiene requires.
Claims (3)
1. compound bio enzymatic degradation Semen Benincasae and processing method thereof, it is characterised in that degrade by adding clear water, compound biological enzyme and glucose in the dry Semen Benincasae of band shell, is then dried and prepares degraded Semen Benincasae；
Described compound biological enzyme is made up of cellulase, hemicellulase, pectase, neutral protease, lipase and phosphate fire-resistant oil enzyme；
Described degraded Semen Benincasae procedure of processing is:
A. by fresh Semen Benincasae cleaning, drying to moisture content less than 10%, the dry Semen Benincasae of band shell are prepared；
B. compound biological enzyme and glucose are added in clear water and stir；
C. dry for band shell Semen Benincasae are added in step B and stir；
D. lucifuge, insulation and anaerobic degradation 4-18 hour under the conditions of 30-60 ° of C；
E. take out, be dried under the conditions of 50-75 ° of C to moisture content less than 10%；
F. pack.
Compound bio enzymatic degradation Semen Benincasae the most according to claim 1 and processing method thereof, is characterized in that: the dry Semen Benincasae of band shell with compound biological enzyme, glucose formula proportion are by weight:
The dry Semen Benincasae of band shell
1000 parts,
Clear water
100-800 part,
Cellulase
0.2-0.5 part,
Hemicellulase
0.2-0.5 part,
Pectase
0.1-0.4 part,
Neutral protease
0.3-0.6 part,
Lipase
0.3-0.6 part,
Phosphate fire-resistant oil enzyme
0.1-0.2 part,
Lysozyme
0.1-0.3 part,
Glucose
50 parts.
Compound bio enzymatic degradation Semen Benincasae the most according to claim 1 and processing method thereof, it is characterized in that: described cellulose enzyme activity 150000u/g, hemicellulase enzyme 150000u/g alive, pectase enzyme 100000u/g alive, neutral protease enzyme 100000u/g alive, lipase activity 50000u/g, phosphate fire-resistant oil enzyme enzyme 50000u/g alive, lysozyme enzyme is lived 50000u/g, is and commercially available meets the qualified products that food hygiene requires.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510034924.8A CN105876507A (en) | 2015-01-25 | 2015-01-25 | Compound biological enzyme degraded melon seeds and processing method thereof |
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|---|---|---|---|
| CN201510034924.8A CN105876507A (en) | 2015-01-25 | 2015-01-25 | Compound biological enzyme degraded melon seeds and processing method thereof |
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| CN105876507A true CN105876507A (en) | 2016-08-24 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111657469A (en) * | 2020-05-27 | 2020-09-15 | 安徽乐方食品有限公司 | Peeled sunflower seeds and processing method thereof |
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2015
- 2015-01-25 CN CN201510034924.8A patent/CN105876507A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111657469A (en) * | 2020-05-27 | 2020-09-15 | 安徽乐方食品有限公司 | Peeled sunflower seeds and processing method thereof |
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