CN105875292A - Method for preventing and treating postharvest diseases of citrus reticulata blanco by combining phosphatidylcholine with hanseniaspora uvarum - Google Patents
Method for preventing and treating postharvest diseases of citrus reticulata blanco by combining phosphatidylcholine with hanseniaspora uvarum Download PDFInfo
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- CN105875292A CN105875292A CN201610225148.4A CN201610225148A CN105875292A CN 105875292 A CN105875292 A CN 105875292A CN 201610225148 A CN201610225148 A CN 201610225148A CN 105875292 A CN105875292 A CN 105875292A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses a method for preventing postharvest diseases of citrus reticulata blanco by combining phosphatidylcholine with hanseniaspora uvarum, and belongs to the field of biological prevention and treatment of postharvest diseases of fruits. The method comprises the steps: inoculating hanseniaspora uvarum into an NYDB culture medium to culture for 20h, centrifuging to obtain thalli, and preparing 1*108 cells/mL of hanseniaspora bacterium suspension comprising phosphatidylcholine (15 mg/mL); uniformly spraying the bacterium suspension to fruits, and naturally air-frying the fruits; placing the fruits in a plastic basket, and after sealing up the plastic basket by a preservative film, storing under the condition of the room temperature (20 DEG C). According to the method disclosed by the invention, phosphatidylcholine is utilized to be combined with hanseniaspora uvarum to process the citrus reticulata blanco; efficacy of biological prevention and treatment of hanseniaspora on the postharvest diseases of citrus reticulata blanco is improved; the method is simple to use, is convenient to operate, has a good effect and is low in cost. The method can replace a chemical disinfectant to prevent and treat postharvest diseases of fruits, avoids damage of use of the chemical disinfectant to people, and has obvious economic benefits and social effects.
Description
Technical field
The present invention relates to a kind of lecithin (phosphatidylcholine) combine Sucus Vitis viniferae and have Hanseniaspora sp. (Hanseniaspora
Uvarum) method improving Citrus postharvest disease preventing efficiency, belongs to biologically controlling postharvest disease of fruit field.
Background technology
Citrus (Citrus reticulata Blanco) belong to platymiscium under Rutaceae, and main species has Fructus Citri Limoniae, Fructus Citri grandis, grapefruit, orange
Skin Citrus, wide etc..The tender succulence of Citrus tissue, rich in multiple nutrients such as vitamin C, mineral, citric acid and dietary polysaccharides
Material, very popular.Citrus be the whole world the first big Citrus be global first big fruit, the tender succulence of fruit tissue and richness
Containing sugar, during its post harvest transport, subject to multiple pathogen infects generation postharvest disease, causes fruit rot to be gone bad,
Economic loss is serious.Citrus are lost between 20-30% because rotting every year, may be up to more than 50% time serious.It is currently known and can draw
The pathogenic bacterium playing Citrus postharvest decay have kind more than 20, wherein by Italy's penicillium sp (Penicillium italicum) and Penicillium digitatum
What (Penicillium digitatum) caused rot for the big Major Diseases of Citrus two, can account for the loss of Citrus postharvest disease time serious
More than 90%, wherein rotten be particularly acute with what Penicillium digitatum caused, this type of pathogenic distribution is extensive, with saprophytic at Organic substance
On mode, produced a large amount of conidium are diffused among air, thus are diffused on fruit, cause fruit rot,
Loss is greatly.
At present, during citrus storage, the preventing and treating of green mould relies primarily on chemical agent, as pressed down mould sound of crying or vomiting, probenazole, Fluoxastrobin, spy
More than gram, o-phenyl phenol sodium, sec-butylamine and benzimidazole germicide etc..In production, frequent and high concentration use chemical agent is made
Pesticide residual toxic dose on fruit and vegetable food is become to increase and threaten the health of the mankind.The report of NAS (NAS)
(1987) pointing out, the application of antibacterial causes harm to human health；In the sales volume of the 9 kinds of neoplastic compounds in the whole America, sterilization
Agent accounts for about 90%, and in whole pesticide of food, the tumorigenesis that antibacterial constitutes 60% is dangerous.Additionally, life-time service
Antibacterial can make pathogen develop immunity to drugs thus reduce prevention effect, according to the investigation of California, USA in the early time, presses down mould
(Imaziall) as benzo miaow class, the regeneration product of sec-butylamine medicine, after using 5 years on Citrus continuously, occurred in that
The anti-of Penicillium digitatum presses down mould bacterial strain.And according to national survey, it being used alone carbendazim area for many years, it is produced by Penicillium digitatum
Having given birth to serious resistance, made Citrus rotting rate go up, drug-fast strain is to thiophanate methyl, benomyl, Apl-Luster etc. three simultaneously
Plant benzimidazole germicide and there is cross tolerance.Therefore, at a lot of developed countries and regions, some chemical bactericides have begun to
Being used by restriction, e.g., several antibacterial such as captan (Captan) and benomyl (benomyl) are prohibited by Environmental Protection in America mechanism
With.
Along with improving constantly of expanding economy and people's living standard, study and develop substitute chemical bactericide safe and nontoxic,
Efficient novel fruits postharvest disease prevention and controls, has great social meaning and wide application prospect.Currently get in the world
More pay attention to carrying out with beneficial microbe the research of biologically controlling postharvest disease of fruit, and it is thought that be most hopeful substituted chemistry and kill
One of direction of microbial inoculum.
In recent years, Biological control has caused the concern of countries in the world, and the application of antagonistic microbe is proved to be a kind of effective
Method.Wherein yeast owing to antagonistic effect is good, do not produce toxin, can advantage becomes with chemical bactericide is used in conjunction with etc.
Study hotspot.Chalitz etc. from the Fructus Citri Limoniae surface isolated one inferior Dbaly yeast of the strain Chinese (Debaryomyces hansenii),
Citrus common green mold, penicilliosis etc. are had significant prevention effect.The report Pantoea agglomerans such as Torres is to Citrus
Penicillium digitatum has good prevention effect.Arras reports Candida famata to be had significantly citrus common green mold
Prevention effect, and inquired into its mechanism of action.Partial agonist bacterium commercialization at present or half commercial applications, such as Candida
Oleophilia isolate I-182 registers at Environmental Protection Agency (EPA) as biofungicide in nineteen ninety-five, and
Produce via U.S. Ecogen company trade metaplasia.Additionally, after South Africa one strain is adopted antagonism yeast Cryptococcus albidus (Cryptococcus
Albidus) commercially produced, trade name Yield Plus.
But up to the present, although the existing semicentennial history of the research of postharvest fruit and vegetable Biological control, but it is applied to raw
The Antagonistic Fungi kind produced is few, a few launch such as only Yield Plus, Biosave and Aspire, and China is current
Antagonistic Fungi is not still had to be applied to commodity production.And for universal, in the postharvest biocontrol of fruit is tested, it is used alone short of money
Antimicrobial controls the effect of fruit postharvest diseases often good not as good as chemical bactericide, it is therefore desirable to improved.Research is sent out
Existing, Antagonistic Fungi is used in combination with some allogenic materials the antagonistic effect that can improve Antagonistic Fungi.
Soybean lecithin is the natural amphoteric surfactant of a kind of function admirable, has good surface activity and distinctive physiology
Activity, and nontoxic, pollution-free, non-stimulated, easily biological-degradable, according to the nature and function of phospholipid, phospholipid frequently as emulsifying agent,
Dispersant, viscosity blender, lubricant, antispray agent, bulking agent, antioxidant and nutrient etc. and be widely used in doctor
The industries such as medicine, chemical industry, light textile work, food, pesticide and cosmetics.Last century Japanese scholars, to lecithin under field condition
Testing, result shows that it is effective for the powdery mildew of Fructus Cucumidis sativi, Fructus Solani melongenae, Fructus Fragariae Ananssae and Capsicum annuum L., and have developed " phospholipid agriculture "
(Lecithinon).Combine phospholipid currently without antagonism yeast and Citrus are adopted the preventing and treating correlational study report of rear green mould.
The present invention selects the Sucus Vitis viniferae of the autonomous isolation identification of this laboratory to have Hanseniaspora sp. to be combined process Citrus chachiensis Hort. with variable concentrations lecithin
Fructus Citri tangerinae, utilizing lecithin to improve Sucus Vitis viniferae has a Hanseniaspora sp. Biological control effect to fruit postharvest diseases, thus reach research and
Develop the purpose of new and effective, nontoxic, safe Citrus antisepsis antistaling agent.
Summary of the invention
The present invention utilizes lecithin to combine Sucus Vitis viniferae Hanseniaspora sp., improves this yeast effect to biologically controlling postharvest disease of fruit
Power.Have with Sucus Vitis viniferae compared with Hanseniaspora sp. individual processing, the yeast preventing and treating to citrus common green mold can be significantly improved in conjunction with processing
Effect, prevents and treats its postharvest decay.The present invention has safety, efficiently, low cost and other advantages, can be widely used for after fruit adopts
The Biological control of disease, reduces the loss that fruit postharvest diseases causes.
The technical solution used in the present invention
The lecithin that the present invention provides combines the method that Sucus Vitis viniferae has Hanseniaspora sp. preventing and treating fruit postharvest diseases, as steps described below
Carry out: after Sucus Vitis viniferae has Hanseniaspora sp. be inoculated into NYDB culture medium culturing 20h, the centrifugal thalline that obtains, preparation phosphorus on ovum
The 1 × 10 of fat8The yeast suspension (the final concentration of 15mg/mL of lecithin) of cells/mL；Bacteria suspension is uniformly sprayed at fruit
On, natural air drying；It is placed in plastic crate, after sealing with preservative film, deposits under the conditions of being placed on room temperature (20 DEG C).
Wherein said NYDB culture medium is: beef extract 8g, yeast extract 5g, glucose 10g, distilled water 1000mL,
PH is natural.
Wherein said bacterial strain Y3 is that Sucus Vitis viniferae has Hanseniaspora sp. Hanseniaspora uvarum, is that this laboratory screening obtains,
Now it is stored in the Institute of Microorganism, Academia Sinica being positioned at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese microorganism strain
Preservation administration committee common micro-organisms center (CGMCC), preservation strain numbered CGMCC No.2.5414, preservation day
Phase is on March 31st, 2016, it is proposed that specific name be that Sucus Vitis viniferae has Hanseniaspora sp. Hanseniaspora uvarum.
The ultimate density of wherein said lecithin is 15mg/mL.
Wherein said containing lecithin 1 × 108The yeast suspension (the final concentration of 15mg/mL of lecithin) of cells/mL, presses
Prepare according to following step: after Sucus Vitis viniferae has Hanseniaspora sp. be inoculated into NYDB culture medium culturing 20h, be centrifuged and obtain bacterium
Body, thalline sterilized water washs three times, and the dilution of thalline sterilized water is prepared as 2 × 108Cells/mL bacteria suspension, with concentration be
The lecithin of twice final concentration 1:1 by volume mixes, and the ultimate density of lecithin is 15mg/mL.
Advantages of the present invention:
(1) Sucus Vitis viniferae used in the present invention has Hanseniaspora sp. (Hanseniaspora uvarum) to be this laboratory screening, its
Antagonistic potencies is strong, safe to the human body harmless.
(2) lecithin wide material sources used in the present invention, cheap, nontoxic.
(3) present invention utilizes lecithin to combine Sucus Vitis viniferae has Hanseniaspora sp. to process fruit, has Hanseniaspora sp. individually to locate with Sucus Vitis viniferae
Reason is compared, and can significantly improve Sucus Vitis viniferae in conjunction with process has the antagonistic activity of Hanseniaspora sp., thus reduces the rotting rate of fruit,
And then reach the purpose that fruit storage is fresh-keeping.
(4) present invention uses lecithin to combine Sucus Vitis viniferae Hanseniaspora sp., can substitute for after chemical bactericide preventing and treating fruit is adopted sick
Evil, it is to avoid use the chemical bactericide harm to people, there is significant economic benefit and social benefit.
Accompanying drawing explanation
Fig. 1 is that lecithin has Hanseniaspora sp. to be combined the control effect to citrus common green mold with Sucus Vitis viniferae.Note: CK: aseptic distillation
Water, i.e. matched group；Y: with the H.uvarum bacteria suspension (1 × 10 of sterile distilled water configuration8cells/mL)；Y+0.1%P: use
The 1 × 10 of lecithin configuration8The H.uvarum bacteria suspension (containing the lecithin of final concentration of 1mg/mL) of cells/ml；
Y+0.5%P: with the 1 × 10 of lecithin configuration8H.uvarum bacteria suspension (the ovum containing final concentration of 5mg/mL of cells/ml
Phospholipid)；Y+1%P: with the 1 × 10 of lecithin configuration8The H.uvarum bacteria suspension of cells/ml is (containing final concentration of 10mg/mL
Lecithin)；Y+1.5%P: with the 1 × 10 of lecithin configuration8The H.uvarum bacteria suspension of cells/ml is (containing final concentration
Lecithin for 15mg/mL)；Y+2%P: with the 1 × 10 of lecithin configuration8The H.uvarum bacteria suspension of cells/ml (contains
The lecithin of final concentration of 20mg/mL)；Y+2.5%P: with the 1 × 10 of lecithin configuration8The H.uvarum of cells/ml
Bacteria suspension (containing the lecithin of final concentration of 25mg/mL)；Y+3%P: with the 1 × 10 of lecithin configuration8The H. of cells/ml
Uvarum bacteria suspension (containing the lecithin of final concentration of 30mg/mL)；Y+4%P: with the 1 × 10 of lecithin configuration8cells/ml
H.uvarum bacteria suspension (containing the lecithin of final concentration of 40mg/mL)；Y+5%P: with the 1 × 10 of lecithin configuration8
The H.uvarum bacteria suspension (containing the lecithin of final concentration of 50mg/mL) of cells/ml.Different letters represent significant difference
(P<0.05)。
Fig. 2 is the impact that lecithin combines that Sucus Vitis viniferae has Hanseniaspora sp. process naturally to rot Citrus.Note: CK: aseptic distillation
Water, i.e. matched group；Y: with the H.uvarum bacteria suspension (1 × 10 of sterile distilled water configuration8cells/mL)；Y+1.5%P:
With lecithin configuration 1 × 108The H.uvarum bacteria suspension (containing the lecithin of final concentration of 15mg/mL) of cells/ml；Different
Letter represents significant difference (P < 0.05).
Detailed description of the invention
The present invention be will be explained in more detail by following embodiment.Following example are merely illustrative, and the present invention is not by this
The restriction of a little embodiments.
Embodiment 1 Sucus Vitis viniferae has Hanseniaspora sp. Y3 (Hanseniaspora uvarum) to be that this laboratory screening obtains, existing preservation
In being positioned at the Institute of Microorganism, Academia Sinica of Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese microorganism strain preservation pipe
Reason committee's common micro-organisms center (CGMCC), preservation strain numbered CGMCC No.2.5414, preservation date is 2016
On March 31, it is proposed that specific name be that Sucus Vitis viniferae has Hanseniaspora sp. Hanseniaspora uvarum.
Cultivation program is: (1) solid activates: Sucus Vitis viniferae has Hanseniaspora sp. be inoculated in NYDA culture medium, 28 DEG C of cultivations
48h；(2) liquid culture: load the NYDB seed culture medium of 50mL in the triangular flask of 250mL, access with inoculating loop
The Sucus Vitis viniferae that one ring activates has Hanseniaspora sp., at 180rpm, cultivates 20h under the conditions of 28 DEG C；(3) centrifugation settling flux:
Above-mentioned Yeast Cultivation mixture, under the conditions of 8000g, is centrifuged 10min, and washs 3 times with sterile distilled water, to remove cultivation
Medium, suspends with sterile distilled water and lecithin respectively, and counts with blood counting chamber, and regulation barm cell concentration is 1 × 108
cells/mL。
Embodiment 2 lecithin combines Sucus Vitis viniferae the Hanseniaspora sp. control effect to citrus common green mold
One) testing program
For the fruit of examination through fine selection, selecting fruit uniformity, surface is without scratch or mechanical damage and disease pest injury as far as possible.With
Aseptic card punch fruit equator goes out punching, and the superficial cut of Citrus is 5mm (diameter) × 3mm (deeply).Each wound equivalent adds
Enter the 30 following treatment fluids of μ L: (1) 1 × 108The Sucus Vitis viniferae of cells/mL has Hanseniaspora sp. suspension (sterile distilled water configuration)；(2)
With lecithin configuration 1 × 108The Sucus Vitis viniferae of cells/mL have Hanseniaspora sp. suspension (lecithin final concentration is respectively 1mg/mL,
5mg/mL, 10mg/mL, 15mg/mL, 20mg/mL, 25mg/mL, 30mg/mL, 40mg/mL, 50mg/mL)；
(3) sterile distilled water.After 2h, then inoculate 30 μ L 1 × 10 respectively to Citrus wound5Penicillium digitatum (the P. of spores/mL
Digitatum) spore suspension.Naturally, after drying, fruit put into plastic crate and seals with preservative film, constant incubator being cultivated (wet
Degree 95%), it is placed in 20 DEG C.Often process and be repeated 3 times, repeat 12 fruits every time.Whole test is repeated 2 times.If through 7 days
Cultivation, record fruit sickness rate, evaluate lecithin with this and combine Sucus Vitis viniferae and have the fungistatic effect of Hanseniaspora sp..
The computing formula of sickness rate is as follows:
The fruit of sickness rate (%)=morbidity/fruit sum × 100%
Two) result of the test
As it is shown in figure 1, Citrus apply lecithin to combine H.uvarum, and to photograph before artificially inoculating Penicillium digitatum spore suspension
Ratio, the Citrus sickness rate of H.uvarum individual processing is substantially less than comparison (P < 0.05), illustrates that Sucus Vitis viniferae has Hanseniaspora sp. to exist
Rear green mould adopted by the Citrus that can effectively control to be caused by Penicillium digitatum at 20 DEG C.Variable concentrations lecithin combines H.uvarum process
Citrus rotting rate be substantially less than H.uvarum individual processing group and matched group (P < 0.05), illustrate that lecithin significantly improves H.
The uvarum prevention effect to citrus common green mold.Especially H.uvarum with 15mg/mL lecithin is combined the Citrus diseases prevention effect processed
Preferably, its sickness rate is only 4.63% to fruit, the rotting rate (33.33%) when substantially less than H.uvarum is used alone.
Embodiment 3 lecithin combines Sucus Vitis viniferae has Hanseniaspora sp. naturally to rot Citrus and the impact of storage quality
One) testing program
Following treatment fluid is uniformly sprayed on Citrus surface: (1) 1 × 108The Sucus Vitis viniferae of cells/mL has Hanseniaspora sp. bacteria suspension (aseptic
Distilled water configures)；(2) with the 1 × 10 of lecithin configuration8The Sucus Vitis viniferae of cells/mL has Hanseniaspora sp. bacteria suspension, and (lecithin is eventually
Concentration is respectively 15mg/mL)；(3) sterile distilled water, as comparison.Citrus are dried naturally, the fruit handled well is put into
Plastic crate preservative film seals.Preserve 1 month under room temperature (20 DEG C).Take out fruit statistics rot fruit number, and observe corruption
Rotten situation and the quality of mensuration fruit.Often processing and be repeated 3 times, repeat 36 fruits every time, whole experiment is repeated 2 times.Ovum phosphorus
The inhibition that fat combines Sucus Vitis viniferae and has Hanseniaspora sp. naturally to rot fruit represents with sickness rate.
1) computing formula of sickness rate is as follows:
The fruit of sickness rate (%)=morbidity/fruit sum × 100%
2) storage quality assay method is as follows:
1. weight-loss ratio: weight method.Weight-loss ratio=(quality after quality-storage before processing)/process front quality × 100%
2. hardness: using the test of TA-XT2i texture analyser, probe diameter is 5mm, before probe test, in test,
The speed of service after test is respectively 4.0mm s-1、1.0mm s-1、5.0mm s-1, Fructus Fragariae Ananssae MTD is 6mm, Fructus Persicae
Fruit and Fructus Mali pumilae MTD are 10mm, and maximum resistance (unit is newton) suffered during probe insertion fruit is defined as
The hardness of fruit.Each fruit is every 120 ° of mensuration 3 times at equator.
3. total dissoluble solid: using WYT (0-80%) saccharometer to be measured, measurement result is expressed as g/100g
Sample.
4. titrable acidity (%): use titrimetry, weigh 20g sarcocarp from 6 fruits, adds distilled water 20ml homogenate,
100ml volumetric flask is washed with 40ml distilled water.It is placed in 80 DEG C of water-bath extraction 30min, stirs once every 5min, take
Go out cooling, be settled to scale.Filter, take 20ml filtrate and be placed in 100ml small beaker, put into a piece of litmus red test paper,
Use 0.1mol l-1NaOH titrates.When litmus paper become blue or solution to the most micro-red time into titration end-point.Result is with containing lemon
Lemon acid percentage composition represents.Often process 3 times and repeat.
5. the mensuration of Vitamin C content: ultraviolet rapid test method.From 6 fruit, take 20g Meat Sample add 20ml 1%
Hydrochloric acid is homogenized, and 50ml volumetric flask is settled to scale.10000 × g, centrifugal 10mim under the conditions of 4 DEG C, supernatant is used for measuring.
Take 0.4ml extracting solution, put in the 10ml scale test tube filling 0.8ml 10%HCl, shake up after distilled water diluting to scale.
With distilled water as blank, at 243nm, measure absorbance.Another draw 0.4ml extracting solution, be separately added into 4ml distilled water and
1.6ml 1moll-1NaOH solution, to 10ml to scale test tube, shakes up, after standing 15min.Add 1.6ml 10%HCl,
Mixing, adds distilled water and is settled to scale.With distilled water as blank, at 243nm, measure absorbance.By liquid to be measured and alkali
Process the A of liquid to be measured243The difference of value, looks into standard curve, calculates ascorbic content in sample.Often process 3 times and repeat.
Two) result of the test
1. testing according to above-mentioned steps, the Citrus nature rotting rate result of statistics is as shown in Figure 2.
As can be seen from Figure 2, Sucus Vitis viniferae has Hanseniaspora sp. individual processing group and Sucus Vitis viniferae to have Hanseniaspora sp. to be combined with lecithin
The Citrus sickness rate of process group is all substantially less than comparison (P < 0.05).Additionally, lecithin has Hanseniaspora sp. to be combined with Sucus Vitis viniferae
Process the control effect Sucus Vitis viniferae to be significantly better than that Citrus are rotted naturally and have Hanseniaspora sp. individual processing group.Illustrate that lecithin shows
Work improves Sucus Vitis viniferae the Hanseniaspora sp. Biological control effect to Citrus postharvest disease.
2. testing according to above-mentioned steps, the orange storing quality results of statistics see table shown in 1.
Table 1 lecithin combines Sucus Vitis viniferae has Hanseniaspora sp. to process the impact on orange storing quality
Note: CK: sterile distilled water, i.e. matched group；Y: with the H.uvarum bacteria suspension (1 × 10 of sterile distilled water configuration8cells/mL)；
Y+1.5%P: with the 1 × 10 of lecithin configuration8The H.uvarum bacteria suspension of cells/ml.Different letters represent significant difference (P < 0.05).
As shown in table 1, compared with the control, H.uvarum individual processing to orange storing quality indices such as weight-loss ratio, hardness,
Soluble solid, titratable acid, Vc content do not have appreciable impact.H.uvarum is combined the Citrus processed and loses with lecithin
Heavily rate is substantially less than comparison and the Citrus weight-loss ratio of H.uvarum individual processing, to remaining storage quality index such as hardness of Citrus,
Soluble solid, Vc and titratable acid do not make a significant impact.
Claims (5)
1. lecithin combines the method that Sucus Vitis viniferae has Hanseniaspora sp. preventing and treating fruit postharvest diseases, it is characterized in that carrying out as steps described below: have Hanseniaspora sp. to activate Sucus Vitis viniferae, Hanseniaspora sp. is had with NYDB culture medium culturing Sucus Vitis viniferae, the centrifugal thalline that obtains, preparation contains the 1 × 10 of lecithin (15 mg/mL)8 Cells/mL yeast suspension；Bacteria suspension is uniformly sprayed on fruit, natural air drying；It is placed in plastic crate, after sealing with preservative film, deposits under the conditions of being placed on room temperature (20 DEG C).
Lecithin the most according to claim 1 combines the method that Sucus Vitis viniferae has Hanseniaspora sp. to use preventing and treating fruit postharvest diseases, it is characterized in that wherein said NYDB culture medium is: yeast extract 5 g, beef extract 8 g, glucose 10 g, distilled water 1000 ml, pH are natural.
Lecithin the most according to claim 1 combines the method that Sucus Vitis viniferae has Hanseniaspora sp. to use preventing and treating fruit postharvest diseases, it is characterized in that wherein said Sucus Vitis viniferae has Hanseniaspora sp., preservation strain numbered CGMCC No. 2.5414, it is proposed that specific name be that Sucus Vitis viniferae has Hanseniaspora sp.Hanseniaspora uvarum 。
Lecithin the most according to claim 1 combines the method that Sucus Vitis viniferae has Hanseniaspora sp. preventing and treating fruit postharvest diseases, it is characterised in that containing the 1 × 10 of lecithin (15 mg/mL)8 Cells/mL yeast suspension, prepares as steps described below: the dilution of thalline sterilized water is prepared as 2 × 108 Cells/mL bacteria suspension, mixes with the lecithin that concentration is twice final concentration 1:1 by volume, and the ultimate density of lecithin is 15
mg/mL。
Lecithin the most according to claim 1 combines the method that Sucus Vitis viniferae has Hanseniaspora sp. preventing and treating fruit postharvest diseases, it is characterised in that wherein said fruit is Citrus.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107868759A (en) * | 2017-12-15 | 2018-04-03 | 北京工商大学 | One plant of grape wine that can effectively prevent and treat postharvest disease of fruits and vegetables has Hanseniaspora valbyensis and its preparation and application method |
| CN114032185A (en) * | 2021-08-24 | 2022-02-11 | 甘肃农业大学 | Hansenula polymorpha culture medium and culture method for grape juice |
| CN114451450A (en) * | 2022-02-24 | 2022-05-10 | 兰州大学 | Hansenula polymorpha capable of antagonizing botrytis cinerea and application thereof |
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| CN114032185A (en) * | 2021-08-24 | 2022-02-11 | 甘肃农业大学 | Hansenula polymorpha culture medium and culture method for grape juice |
| CN114451450A (en) * | 2022-02-24 | 2022-05-10 | 兰州大学 | Hansenula polymorpha capable of antagonizing botrytis cinerea and application thereof |
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