CN105866937A - Method for determining microscope objective lens focus for visible light through laser conversion - Google Patents
Method for determining microscope objective lens focus for visible light through laser conversion Download PDFInfo
- Publication number
- CN105866937A CN105866937A CN201610410563.7A CN201610410563A CN105866937A CN 105866937 A CN105866937 A CN 105866937A CN 201610410563 A CN201610410563 A CN 201610410563A CN 105866937 A CN105866937 A CN 105866937A
- Authority
- CN
- China
- Prior art keywords
- crystal
- microscopical
- microscope objective
- laser
- microscope
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/241—Devices for focusing
- G02B21/245—Devices for focusing using auxiliary sources, detectors
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
Abstract
The invention relates to the technical field of microscope objective lens focus determination, in particular to a method for determining a microscope objective lens focus for visible light through laser conversion. The method is characterized in that invisible laser is used as a light source of a microscope; a conversion luminous crystal is put on an objective table of the microscope, and is aligned with the center of a light passing hole of the microscope; the distance between the objective lens of the microscope and the conversion luminous crystal is changed, so that the invisible laser is irradiated on the conversion luminous crystal and is focused in the conversion luminous crystal to generate a colored visible light point; the visible light point is the microscope objective lens focus; the located position of the visible light point is the position of the microscope objective lens focus. The method has the advantages that the space position of the microscope objective lens focus can be fast, intuitively and accurately determined, so that a tested object is regulated to the located focal plane position of the microscope objective lens focus in the later period; the clear image of the tested object in the microscope is simply regulated out.
Description
Technical field
The present invention relates to determine the technical field of microscope objective focus, particularly relate to a kind of apply laser to be converted to the method that visible ray determines microscope objective focus.
Background technology
Optical microscope is to utilize optical principle, present human eye the optical instrument of unresolvable fine structure.Based on basic geometric optical theory, if optical microscope focal position under different multiples object lens can be determined the most easily, then actual mechanical process just can relatively quickly adjust testee image clearly.In prior art, common microscopical autofocus system is typically with computer as control core, uses motor etc. as control parts of motion, selects CCD or CMOS camera to combine software algorithm and be focused analyzing and motor control；This kind of theoretical method calculating process is loaded down with trivial details, and equipment cost is high, and use occasion is limited, it is impossible to be applied to experimentation at ordinary times.
Summary of the invention
Present invention aims to the deficiencies in the prior art, it is provided that a kind of reasonable in design, easy and simple to handle, intuitively, efficiently, economic application laser is converted to the method that visible ray determines microscope objective focus.
For achieving the above object, the present invention is by the following technical solutions:
A kind of laser is applied to be converted to the method that visible ray determines microscope objective focus, the method is using invisible laser as microscopical light source, conversion luminescent crystal is placed on microscopical object stage, and the light hole central authorities of aligming microscope, change the distance between microscopical object lens and conversion luminescent crystal, invisible laser is made to be radiated on conversion luminescent crystal, at conversion luminescent crystal inner focusing, coloured visible point of light occurs, this visible point of light is microscope objective focus, and this visible point of light position is microscope objective focal position.
As preferably, described luminescent crystal of changing is into up-conversion luminescence crystal or lower conversion luminescent crystal.
As preferably, described up-conversion luminescence crystal is er-doped yttrium vanadate crystal, and the wavelength of described invisible laser is 980nm, and described visible point of light is green.The er-doped yttrium vanadate crystal that the present invention uses can be converted into high-energy photon lower energy photon, and luminous efficiency is high, and display light path is clear, has good engineering properties and physical property, at the advantageous property shown by all many-sides, is especially suited as laser crystal material；When the invisible laser of the naked eyes that wavelength is 980nm is radiated on er-doped yttrium vanadate crystal, it can be seen that demonstrate the naked eyes visible green photon that wavelength is 553nm inside er-doped yttrium vanadate crystal, by changing the distance between microscopical object lens and er-doped yttrium vanadate crystal, make the internal visible point of light that green occurs of er-doped yttrium vanadate crystal, this green visible point is microscope objective focus, then by distance A between object lens and the object stage of measuring microscope, or distance B between the light source of measuring microscope and object stage, so that it is determined that microscopical object focal point position spatially.
Further, described er-doped yttrium vanadate crystal uses czochralski method to prepare, er-doped yttrium vanadate crystal concentration of Er be 0.2-1.2mol%.The er-doped yttrium vanadate crystal that the present invention uses concentration of Er to be 0.2-1.2mol% makes green glow have longer fluorescence lifetime, and good resolution, low cost.
As preferably, described lower luminescent crystal of changing is into cadmium sulfide crystal, and the wavelength of described visible laser is 325nm, and described visible point of light is aeruginous.The cadmium sulfide crystal that the present invention uses can be converted into lower energy photon high-energy photon, when the invisible laser of the naked eyes that wavelength is 325nm is radiated on cadmium sulfide crystal, it can be seen that cadmium sulfide crystals demonstrates macroscopic aeruginous photon, by changing the distance between microscopical object lens and er-doped yttrium vanadate crystal, make cadmium sulfide crystals that glaucous visible point of light occur, this aeruginous visible point of light is microscopical object focal point, then by distance A between object lens and the object stage of measuring microscope, or distance B between the light source of measuring microscope and object stage, so that it is determined that microscopical object focal point position spatially.
As preferably, the described microscopical object lens of change with the method for the spacing of conversion luminescent crystal are, the conversion luminescent crystal on microscopical object stage and object stage is kept to maintain static, lift by regulating microscopical lens barrel, microscopical object lens are driven to move, to change the distance between microscopical object lens and conversion luminescent crystal.
As preferably, the described microscopical object lens of change with the method for the spacing of conversion luminescent crystal are, microscopical object lens are kept to maintain static, by regulating microscopical lifting of object loading table, the conversion luminescent crystal on object stage is driven to move, to change the distance between microscopical object lens and conversion luminescent crystal.
The present invention uses above technical scheme, on microscope, invisible laser can be converted to the technology of visible ray to determine microscopical object focal point position spatially by application conversion luminescent crystal, make the later stage when using microscopic object, as long as testee is regulated to microscope objective focus place focal plane position, just can be quick, adjust the picture rich in detail of testee in microscope accurately, thus greatly reduce microscope focal adjustments difficulty, save the plenty of time, reduce labor intensity, experiment and the speed analyzed can be improved, reduce the manual operation impact on micro-imaging quality, the method is easy and simple to handle, economical and practical, be suitable to promote.
Accompanying drawing explanation
In conjunction with accompanying drawing, the present invention is further elaborated:
Fig. 1 is that microscope objective focal position of the present invention determines principle schematic；
Fig. 2 is to apply 980nm laser to irradiate er-doped yttrium vanadate crystal to determine the photo of microscope objective focus in the embodiment of the present invention 1；Visible point of light green in er-doped yttrium vanadate crystal in figure is microscopical object focal point；
Fig. 3 is the embodiment of the present invention 1 er-doped yttrium vanadate crystal photoluminescence spectra figure.
Detailed description of the invention
As shown in one of Fig. 1-3, a kind of laser is applied to be converted to the method that visible ray determines microscope objective focus, the method is using invisible laser as microscopical light source 1, conversion luminescent crystal 2 is placed on microscopical object stage, and the light hole central authorities of aligming microscope, change the distance between microscopical object lens 3 and conversion luminescent crystal, invisible laser is made to be radiated on conversion luminescent crystal 2, focus in luminous brilliant 2 bodies of conversion and coloured visible point of light 4 occurs, this visible point of light 4 is microscope objective focus, this visible point of light position is microscope objective focal position.
As preferably, described luminescent crystal of changing is into up-conversion luminescence crystal or lower conversion luminescent crystal.
As preferably, described up-conversion luminescence crystal is er-doped yttrium vanadate crystal, and the wavelength of described invisible laser is 980nm, and described visible point of light is green.
Further, described er-doped yttrium vanadate crystal uses czochralski method to prepare, er-doped yttrium vanadate crystal concentration of Er be 0.2-1.2mol%.The er-doped yttrium vanadate crystal that the present invention uses concentration of Er to be 0.2-1.2mol% makes green glow have longer fluorescence lifetime, and good resolution, low cost.
As preferably, described lower luminescent crystal of changing is into cadmium sulfide crystal, and the wavelength of described visible laser is 325nm, and described visible point of light is aeruginous.
As preferably, the described microscopical object lens of change with the method for the spacing of conversion luminescent crystal are, the conversion luminescent crystal on microscopical object stage and object stage is kept to maintain static, lift by regulating microscopical lens barrel, microscopical object lens are driven to move, to change the distance between microscopical object lens and conversion luminescent crystal.
As preferably, the described microscopical object lens of change with the method for the spacing of conversion luminescent crystal are, microscopical object lens are kept to maintain static, by regulating microscopical lifting of object loading table, the conversion luminescent crystal on object stage is driven to move, to change the distance between microscopical object lens and conversion luminescent crystal.
Embodiment 1
1), preparation er-doped yttrium vanadate crystal: use czochralski method prepare er-doped yttrium vanadate crystal, er-doped yttrium vanadate crystal concentration of Er be 0.2-1.2mol%.
null2)、Application laser is converted to visible ray and determines microscope objective focus: method is that the invisible laser using wavelength as 980nm is as microscopical light source，Er-doped yttrium vanadate crystal is placed on microscopical object stage as up-conversion luminescence crystal，And the light hole central authorities of aligming microscope，Invisible laser is made to be radiated on er-doped yttrium vanadate crystal，Occur in er-doped yttrium vanadate crystal that wavelength is the macroscopic green photon of 553nm，The er-doped yttrium vanadate crystal on microscopical object stage and object stage is kept to maintain static，Lift by regulating microscopical lens barrel，Microscopical object lens are driven to move，To change the distance between microscopical object lens and er-doped yttrium vanadate crystal，Make er-doped yttrium vanadate crystal inner focusing that green visible point of light to occur，This green visible point is microscope objective focus，Now，Distance A between object lens and the object stage of measuring microscope，So that it is determined that microscopical object focal point position spatially.
3), microscopic testee is used: method is to use microscope conventional practices, microscopical object stage is kept to maintain static, testee is placed on microscopical object stage, lift by regulating microscopical lens barrel, microscopical object lens are driven to move, making microscopical object lens is A with the spacing of object stage, so that testee is positioned at microscope objective focus place focal plane position, and then quick, intuitive and accurate can adjust the picture rich in detail of testee in microscope.
Embodiment 2
1) cadmium sulfide crystal, is prepared: use czochralski method to prepare cadmium sulfide crystal.
null2)、Application laser is converted to visible ray and determines microscope objective focus: method is that the invisible laser using wavelength as 325nm is as microscopical light source，Cadmium sulfide crystal is placed on microscopical object stage as lower conversion luminescent crystal，And the light hole central authorities of aligming microscope，Invisible laser is made to be radiated on cadmium sulfide crystal，Macroscopic aeruginous photon occurs in cadmium sulfide crystal，Microscopical object lens are kept to maintain static，By regulating microscopical lifting of object loading table，The cadmium sulfide crystal on object stage is driven to move，To change the distance between microscopical object lens and cadmium sulfide crystal，Invisible laser is made to be radiated on cadmium sulfide crystal，There is glaucous visible point of light in cadmium sulfide crystal inner focusing，This aeruginous visible point of light is microscope objective focus，Now，Distance B between light source and the object stage of measuring microscope，So that it is determined that microscopical object focal point position spatially.
3), microscopic testee is used: method is to use microscope conventional practices, microscopical object lens are kept to maintain static, testee is placed on microscopical object stage, by regulating microscopical lifting of object loading table, the testee on object stage is driven to move, making the distance between microscopical light source and object stage is B, so that testee is positioned at microscope objective focus place focal plane position, and then quick, intuitive and accurate can adjust the picture rich in detail of testee in microscope.
Above description should not have any restriction to protection scope of the present invention.
Claims (7)
1. apply laser to be converted to the method that visible ray determines microscope objective focus for one kind, it is characterized in that: the method is using invisible laser as microscopical light source, conversion luminescent crystal is placed on microscopical object stage, and the light hole central authorities of aligming microscope, change the distance between microscopical object lens and conversion luminescent crystal, invisible laser is made to be radiated on conversion luminescent crystal, at conversion luminescent crystal inner focusing, coloured visible point of light occurs, this visible point of light is microscope objective focus, this visible point of light position is microscope objective focal position.
The most according to claim 1 a kind of laser is applied to be converted to the method that visible ray determines microscope objective focus, it is characterised in that: described changes luminescent crystal into up-conversion luminescence crystal or lower conversion luminescent crystal.
The most according to claim 2 a kind of laser is applied to be converted to the method that visible ray determines microscope objective focus, it is characterized in that: described up-conversion luminescence crystal is er-doped yttrium vanadate crystal, the wavelength of described invisible laser is 980nm, and described visible point of light is green.
4. a kind of apply laser to be converted to the method that visible ray determines microscope objective focus according to what claim 3 was stated, it is characterised in that: described er-doped yttrium vanadate crystal uses czochralski method to prepare, and the concentration of Er of er-doped yttrium vanadate crystal is 0.2-1.2mol%.
The most according to claim 2 a kind of laser is applied to be converted to the method that visible ray determines microscope objective focus, it is characterized in that: described lower luminescent crystal of changing is into cadmium sulfide crystal, the wavelength of described visible laser is 325nm, and described visible point of light is aeruginous.
The most according to claim 1 a kind of laser is applied to be converted to the method that visible ray determines microscope objective focus, it is characterized in that: the described microscopical object lens of change with the method for the spacing of conversion luminescent crystal are, the conversion luminescent crystal on microscopical object stage and object stage is kept to maintain static, lift by regulating microscopical lens barrel, microscopical object lens are driven to move, to change the distance between microscopical object lens and conversion luminescent crystal.
The most according to claim 1 a kind of laser is applied to be converted to the method that visible ray determines microscope objective focus, it is characterized in that: the described microscopical object lens of change with the method for the spacing of conversion luminescent crystal are, microscopical object lens are kept to maintain static, by regulating microscopical lifting of object loading table, the conversion luminescent crystal on object stage is driven to move, to change the distance between microscopical object lens and conversion luminescent crystal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610410563.7A CN105866937B (en) | 2016-06-12 | 2016-06-12 | A method of it being converted to visible light using laser and determines micro objective focus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610410563.7A CN105866937B (en) | 2016-06-12 | 2016-06-12 | A method of it being converted to visible light using laser and determines micro objective focus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105866937A true CN105866937A (en) | 2016-08-17 |
| CN105866937B CN105866937B (en) | 2018-07-17 |
Family
ID=56650102
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610410563.7A Expired - Fee Related CN105866937B (en) | 2016-06-12 | 2016-06-12 | A method of it being converted to visible light using laser and determines micro objective focus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105866937B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113008849A (en) * | 2021-02-07 | 2021-06-22 | 电子科技大学 | Ultraviolet-near infrared broadband micro-region photoluminescence spectrum testing device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003185914A (en) * | 2001-12-21 | 2003-07-03 | Nikon Corp | Focus detector, optical device equipped with the same and focus detecting method |
| CN1887498A (en) * | 2006-07-28 | 2007-01-03 | 北京工业大学 | Display method of converting infrared laser path into visible light |
| CN101294902A (en) * | 2008-06-12 | 2008-10-29 | 复旦大学 | Conversion illuminating microscope on mesomeric state laser pump |
| CN101438147A (en) * | 2006-05-31 | 2009-05-20 | 奥林巴斯株式会社 | Biological specimen imaging method and biological specimen imaging apparatus |
| US7576307B2 (en) * | 2007-04-30 | 2009-08-18 | General Electric Company | Microscope with dual image sensors for rapid autofocusing |
-
2016
- 2016-06-12 CN CN201610410563.7A patent/CN105866937B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003185914A (en) * | 2001-12-21 | 2003-07-03 | Nikon Corp | Focus detector, optical device equipped with the same and focus detecting method |
| CN101438147A (en) * | 2006-05-31 | 2009-05-20 | 奥林巴斯株式会社 | Biological specimen imaging method and biological specimen imaging apparatus |
| CN1887498A (en) * | 2006-07-28 | 2007-01-03 | 北京工业大学 | Display method of converting infrared laser path into visible light |
| US7576307B2 (en) * | 2007-04-30 | 2009-08-18 | General Electric Company | Microscope with dual image sensors for rapid autofocusing |
| CN101294902A (en) * | 2008-06-12 | 2008-10-29 | 复旦大学 | Conversion illuminating microscope on mesomeric state laser pump |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113008849A (en) * | 2021-02-07 | 2021-06-22 | 电子科技大学 | Ultraviolet-near infrared broadband micro-region photoluminescence spectrum testing device |
| CN113008849B (en) * | 2021-02-07 | 2022-08-02 | 电子科技大学 | Ultraviolet-near infrared broadband micro-region photoluminescence spectrum testing device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105866937B (en) | 2018-07-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104062750B (en) | A kind of two-photon fluorescence stimulated emission differential super-resolution microscopic method and device | |
| CN104965384B (en) | Optical projection system, light-source system and light source assembly | |
| CN103597396B (en) | Wide field microscope illumination system and Both wide field illumination method | |
| CN105004704B (en) | The upper conversion nano crystalline substance new application of neodymium ion sensitization and high-resolution multi-photon microscopic system | |
| CN102854723B (en) | Light-emitting device and projection apparatus | |
| CN208283710U (en) | Lighting system and projection arrangement | |
| JP2005128493A5 (en) | ||
| CN1856735A (en) | Lighting device, image display unit and projector | |
| EP2952861A1 (en) | Apparatus and method for single shot measurement of the m2 parameter of a laser beam | |
| Wang et al. | Advance on high power diode laser coupling | |
| JP2016534395A (en) | High resolution 3D fluorescence microscopy | |
| CN105824176A (en) | Curved surface reflecting type ultra-short focal projection lens based on mems | |
| KR101138221B1 (en) | Camera test system for testing shading and color balancing | |
| CN105866937A (en) | Method for determining microscope objective lens focus for visible light through laser conversion | |
| Laurence et al. | Quasi-continuum photoluminescence: Unusual broad spectral and temporal characteristics found in defective surfaces of silica and other materials | |
| CN106970060A (en) | Utilize the measuring method of the long phosphorescence spectrum of femtosecond laser parallel micromachining system induction | |
| JP2016537682A (en) | Method for selecting fluorescent powder optical film applied to backlight module and backlight module | |
| CN105204151A (en) | Lighting device and method | |
| CN103335988B (en) | Line based on post lens focus scanning stimulated emission depletion microscopic imaging device | |
| CN108318468B (en) | Counting system for rapidly counting fluorescent dye staining particles in liquid sample | |
| CN204165513U (en) | The efficient image measuring device of sweep type | |
| CN106568754A (en) | Optical system used for measuring liquid sample multiphoton fluorescence spectrum | |
| CN204044069U (en) | Micro-confocal fluorescent system | |
| WO2014023067A1 (en) | Light-emitting device and related projection system | |
| CN103884656B (en) | Micro-nano optical spectrum imaging device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180717 |