CN105862144A - Efficient preparing method for fibroin nano-fiber and application of fibroin nano-fiber - Google Patents
Efficient preparing method for fibroin nano-fiber and application of fibroin nano-fiber Download PDFInfo
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- CN105862144A CN105862144A CN201610204055.3A CN201610204055A CN105862144A CN 105862144 A CN105862144 A CN 105862144A CN 201610204055 A CN201610204055 A CN 201610204055A CN 105862144 A CN105862144 A CN 105862144A
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0015—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
- A61L24/108—Specific proteins or polypeptides not covered by groups A61L24/102 - A61L24/106
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F4/00—Monocomponent artificial filaments or the like of proteins; Manufacture thereof
- D01F4/02—Monocomponent artificial filaments or the like of proteins; Manufacture thereof from fibroin
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M10/00—Physical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. ultrasonic, corona discharge, irradiation, electric currents, or magnetic fields; Physical treatment combined with treatment with chemical compounds or elements
- D06M10/02—Physical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. ultrasonic, corona discharge, irradiation, electric currents, or magnetic fields; Physical treatment combined with treatment with chemical compounds or elements ultrasonic or sonic; Corona discharge
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
- A61L2300/406—Antibiotics
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/02—Natural fibres, other than mineral fibres
- D06M2101/10—Animal fibres
- D06M2101/12—Keratin fibres or silk
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Abstract
The invention provides an improved efficient preparing method for fibroin nano-fiber to overcome the defect existing in ultrasonic preparing of the fibroin nano-fiber. The efficient preparing method is characterized by mainly comprising the following steps that a convex array or linear array probe is used, an ultrasonic probe and ultrasonic fibroin fiber are both immersed in a solution, the power ranges about from 200 W to 1,750 W, the frequency ranges from 5 Hz to 5 MHz, the ultrasonic duration ranges from 1 h to 24 h, stopping is conducted for 1-10 s every time when the ultrasonic interval time exceeds 3-60 s, and the temperature of an ultrasonic solution is controlled to range from 0 DEG C to 90 DEG C in the process; the ultrasonic fibroin fiber is fixed to a square hollow or circular hollow plane clamp, the vertical distance between the ultrasonic probe and the plane where the fibroin fiber is located ranges from 0.1 cm to 5 cm, the clamp where the ultrasonic fibroin fiber is located is horizontally moved at the speed of 0-5 cm/Min, and accordingly, a fibroin nano-fiber material is prepared. The prepared fibroin nano-fiber has good hydrophilia, vapor penetrating performance and medicine slow release performance, and the application of the fibroin nano-fiber in the aspect of surgical dressing is protected.
Description
Technical field
The present invention relates to high efficiency preparation method and the application thereof of the fibroin nanofiber of a kind of improvement, belong to bio-medical material
Material technical field.
Background technology
Along with to the further investigation of silkworm silk structural behaviour and the exploitation of Novel Materials of Silk, both at home and abroad to the application of silkworm silk just
From traditional field of textiles to field expansions such as organizational project, drug release, biological medicine, cosmetics, food.Fibroin Nanowire
Dimension is a kind of typical nanometer silkworm silk material, in addition to possessing the premium properties that natural silk cellulose fiber is had, also has bigger
Specific surface area, high surface energy, small-size effect, skin effect.
Preparing now fibroin nanofiber mainly to be prepared by method of electrostatic spinning, the method needs with regenerated silk fibroin
Solution is raw material, and the preparation of regenerated silk fibroin solution needs through dissolving and dialysis technique, complex process, cannot volume production.
Ultrasonic is a kind of simple method efficient, that directly produce fibroin nanofiber from fibroin fiber, has technique
Flow process is simple, is suitable for the advantages (patent 200510086251.7) such as extensive preparation.Owing to fibroin fiber is as long fiber material
Random Brownian movement can not be done in the solution, by ultrasound wave own physical characteristic, such as the directivity of sonic transmissions, reflect, roll over
Penetrating, scatter and absorb the sound wave caused to affect with range attenuation etc., there is a part and occurs in the fibroin fiber being ultrasonically treated
Division, and the ultrasonic non-uniformity problem that another part is barely affected, thus cause ultrasonic method to prepare fibroin nanofiber
Point fine uneven and productivity is extremely low.For this problem, the ultrasonic method preparing fibroin nanofiber is carried out by this patent
Optimize, and the character that the fibroin nanofiber prepared by technique after optimizing is applied to medical dressing is characterized.
Summary of the invention
Present invention aims to the ultrasonic problem preparing the existence of fibroin nanofiber, it is provided that one efficiently prepares silk
The method of element nanofiber, with the shortcoming and defect overcoming prior art to exist.
Solve the technical problem that required for the present invention, a kind of method efficiently preparing fibroin nanofiber, can be by following
Technical scheme realizes:
It is characterized in that using convex battle array or linear array probe, ultrasonic probe and all being immersed in solution by ultrasonic fibroin fiber, power is big
About between 200W ~ 1750W, frequency is 5 KHz～5 megahertzs, and the ultrasonic persistent period is 1～24 hour, during ultrasonic interval
Between stop 1 ~ 10s for surpassing 3～60s, during control by ultrasonic solution temperature between 0 DEG C～90 DEG C；Will be by ultrasonic fibroin fiber
Be fixed on the level clamp of square hollow or circular hollow, control ultrasonic probe to fibroin fiber place plane vertical away from
From for 0.1～5cm, move horizontally by the plane at ultrasonic fibroin fiber place with the speed of 0～5cm/Min, thus prepare
Fibroin nano-fiber material.
Described fibroin fiber includes that silkworm, Antherea pernyi Guerin-Meneville, giant silkworm, Bombyx mandarina Moore and transgenic silkworm are weaved silk, after cocoond case degumming
That is processed into forms fiber-like and silkworm silk fabric material with fibroin albumen for main body.
Described solution refers to any one of weak acid, weak base, pure water and ultra-pure water or multiple, or is carbonic acid
In sodium, tertiary sodium phosphate, sodium dihydrogen phosphate and disodium hydrogen phosphate any 2～3 kind of mixed solution.
Described ultrasonic power and frequency are to ensure that the size of prepared fibroin nanofiber is than more uniform and 90%
Diameter Distribution is within 300nm.
Described fixing fibroin fiber is for the ease of controlling by thermoplastic material relative to the direction of ultrasonic probe and distance, making
Ultrasound wave continuingly acts on fibroin fiber from specific direction, and the energy making ultrasound wave produce is fine at fibroin over time and space
Dimension table face forms synergistic effect, it is to avoid point fine non-uniform phenomenon existing for the fibroin fiber surface of supersound process.
It is described that to move horizontally fibroin fiber with given pace be the scale in order to realize fibroin nanofiber in technique
Change preparation.
The present invention by realizing the diameter fibroin fiber at micro-meter scale to the Precise control of ultrasonic process conditions
Directly hundreds of fibroin nanofibers are split into from single under ultrasonication.
Fibroin nanofiber prepared by the present invention has good hydrophilic, water vapo(u)r transmission energy and controlled drug release
Can, this patent protects its application in terms of medical dressing.
Accompanying drawing explanation
Fig. 1. in the case of ultrasound condition is not optimised, scanning electron microscope diagram after point fibre after single fiber is ultrasonic；
Fig. 2. the scanning electron microscope microscope figure of sample after using the optimization ultrasonic method in this patent to process, individual thread cellulose fiber can
Hundreds of nanofibers are become with amitosis；
Fig. 3. use the optimization ultrasonic processing method in this patent, the diameter Distribution interval statistics of prepared fibroin nanofiber
Figure；
Fig. 4. in the case of ultrasound condition is not optimised, ultrasonic after, fine inhomogeneities is divided on fibroin fiber surface；
Fig. 5. the scanning electron microscope microscope figure of sample after using the optimization ultrasonic method in this patent to process, fibroin fiber divides fine table
Face presents uniformity；
Fig. 6. the scanning electron microscope microscope figure of sample after using the optimization ultrasonic method in this patent to process, fibroin fiber divides fine table
Face presents uniformity；
Fig. 7. use the optimization ultrasonic processing method in this patent, the diameter Distribution interval statistics of prepared fibroin nanofiber
Figure；
Fig. 8. the scanning electron microscope microscope figure of sample after using the optimization ultrasonic method in this patent to process, it is possible to achieve fibroin is received
Prepared by rice fiber scale；
Fig. 9. diameter 2 μ L drop measures lower fibroin fiber and contrasts with fibroin nanofiber static contact angle；
Figure 10. under diameter 8 μ L drop, fibroin fiber contrasts with fibroin nanofiber static contact angle；
Figure 11. fibroin fiber, fibroin nanofiber and Bacterial cellulose (BC-Foam) water vapo(u)r transmission can contrast；
Figure 12. fibroin fiber and release (CRP) percentage ratio contrast of fibroin nanofiber drug accumulation.
Detailed description of the invention
Embodiment 1 single fiber is point fine situation contrast before and after ultrasound condition optimizes
(1) in the case of ultrasound condition is not optimised, point fine situation after individual thread cellulose fiber supersound process
The method using patent 200510086251.7, puts into fibroin fiber in ultrasonic solution, with 1250 W power, 20 KHz
Frequency, surpasses 3 seconds and stops 1 second, and the time is 15 min, supersound process fibroin fiber, the ultrasonic knot that single-silk fiber surface divides fibre
Such as scanning electron microscope diagram as shown in Figure 1, fibroin nanofiber successively comes off from fiber surface fruit with banding, the most easily makes
The fibroin nanofiber becoming to be introduced into solution is dissolved in solution under ul-trasonic irradiation, along with ultrasonic time extends, ultrasonic
Prepare the productivity of fibroin nanofiber less than 1% and without the trend significantly increased.By accompanying drawing 1 it can be seen that ultrasound condition is the most excellent
In the case of change, fibroin nanofiber from fibroin fiber surface layer-by-layer separate, and ultrasound wave to fibroin fiber, (diameter is micro-in 10～30
Between meter) divide a fine impact to rest in the micrometer range of surface 1.
(2) this patent is used to optimize under ultrasound condition, point fine situation after individual thread cellulose fiber supersound process
The method using this patent, puts into fibroin fiber in ultrasonic solution, with convex array probe, with 1250 W power, 20 KHz
Frequency, the ultrasonic persistent period is 15 minutes, surpasses 3 seconds and stops 1 second, during control by ultrasonic solution temperature to be 50 DEG C, will be ultrasonic
Fibroin fiber is fixed on circular hollow level clamp, and controlling ultrasonic probe is 1cm to fibroin fiber place plane vertical dimension,
Supersound process fibroin fiber, single-silk fiber surface divides the result scanning electron microscope diagram of fibre as shown in Figure 2.From figure
It can be seen that owing to ultrasound wave continuingly acts on fibroin fiber from specific direction, individual thread cellulose fiber can become with amitosis
Hundreds of nanofibers, it is to avoid point fine non-uniform phenomenon existing for the fibroin fiber surface of supersound process, make fibroin Nanowire
The productivity of dimension is higher than 50%.Accompanying drawing 3 can be seen that prepared fibroin nanofiber diameter accounts for 99% at 300 below nm.
Embodiment 2 fibroin fiber optimizes front and rear surfaces at ultrasound condition and divides fine inhomogeneities contrast
(1) use patent 200510086251.7 method, fibroin fiber is put in ultrasonic solution, with 1750 W power, 20
KHz frequency, surpasses 3 seconds and stops 1 second, and the time is 20 min, supersound process fibroin fiber, the ultrasonic result that silk fiber surface is divided fibre
As it can be seen, by ultrasonic fibroin fiber surface as shown in Figure 4.Owing to the instantaneous burst of ultrasonication is relative with fibroin fiber
The position of ultrasonic probe is different, by ultrasonication to fiber be easy to a point fibre effect, and go back nubbin fiber, it appears that
It is not affected by the impact of ultrasonication.This part of fiber occurs a point fibre to act on, and the unaffected fibroin of another part
Fiber divides fine non-uniform phenomenon to have a strong impact on the productivity of ultrasonic generation fibroin nanofiber.In 20 minutes, fibroin nanofiber produces
Rate is only 1.2%.
(2) method using this patent, puts into fibroin fiber in ultrasonic solution, uses convex array probe, by 1750 W merits
Rate, 20 KHz frequencies, the ultrasonic persistent period is 15 minutes, surpasses 3 seconds and stops 1 second, during control by ultrasonic solution temperature to be 50 DEG C,
To be fixed on circular hollow level clamp by ultrasonic fibroin fiber, control ultrasonic probe to fibroin fiber place plane vertically away from
From for 0.5cm, supersound process fibroin fiber.Owing to securing fibroin fiber, ultrasound wave continuingly acts on silk from specific direction
Cellulose fiber, can be realized being uniformly distributed of nanofiber by ultrasonic fiber surface, it is to avoid the fibroin fiber surface of supersound process is deposited
Point fine non-uniform phenomenon, make the productivity of fibroin nanofiber higher than 50%, generate fibroin nanofiber shown in accompanying drawing 5 and putting
Scanning electron microscope (SEM) photograph when big 100 times, 1000 times and 10000 times, the diameter 90% of gained fibroin nanofiber is distributed in 300nm
Below.
Embodiment 3 uses the method for this patent, fibroin fiber is put into sodium carbonate and tertiary sodium phosphate mixing ultrasonic solution is pre-
After processing 5 hours, will be fixed on square hollow level clamp by ultrasonic fibroin fiber, control ultrasonic probe to fibroin fiber institute
It is 0.5cm in plane vertical dimension, supersound process fibroin fiber, use convex array probe, by 500 W power, 20 KHz frequencies, super
The sound persistent period is 15 minutes, surpasses 3 seconds and stops 1 second, during control by ultrasonic solution temperature to be 60 DEG C.Gained fibroin nanofiber
Productivity higher than 50%.As shown in accompanying drawing 6 and accompanying drawing 7, generated fibroin nanofiber is when amplifying 100 times and 10000 times
Scanning electron microscope (SEM) photograph, the diameter Distribution of gained fibroin nanofiber accounts for 99% at below 300nm.
Embodiment 4 uses the method for this patent, is put into by fibroin fiber in sodium carbonate and tertiary sodium phosphate mixing ultrasonic solution,
To be fixed on the level clamp of circular hollow by ultrasonic fibroin fiber, control ultrasonic probe vertical to fibroin fiber place plane
Distance is 0.5cm, moves horizontally by the plane at ultrasonic fibroin fiber place with the speed of 0.2cm/Min, uses convex array probe, uses
500W power, 20KHz frequency, surpass 4 seconds and stop 1 second, during control by ultrasonic solution temperature to be 60 DEG C, the ultrasonic persistent period is
1h, can obtain the fibroin nano-fiber cloth (black circles inner region in accompanying drawing 8B) that diameter is about 8cm.
Fibroin nanofiber prepared by embodiment 5 this method compares with the hydrophilic and hydrophobic confrontation of fibroin fiber
Random 5 pieces of samples of clip, the water droplet 5 dripping diameter 2 μ l and 8 μ l respectively drips to dielectric surface difference place, passes through after 5s
Instrument automatically determines water droplet profile and contacts angulation with dielectric surface to determine the hydrophobe character of dielectric surface.Left and
Right represents the size of left and right contact angle θ 1 and θ 2 respectively.More than 150 degree, usual angle shows that dielectric surface has super-hydrophobic
Property, contact angle contrast is as follows.Contact angle is the important parameter characterizing solid plane material surface hydrophilic performance.Material surface
Contact angle is the least, and its hydrophilic is the best, otherwise, its hydrophilic is the poorest.Accompanying drawing 9 and accompanying drawing 10 are respectively diameter 2 μ l and 8 μ l water
System after touching fiber surface 1.0s of dripping takes the contact angle data of drop automatically.As can be seen from the table, diameter 2 μ l and 8
μ l water droplet fibroin fiber surface after degumming is subcircular, and static contact angle is respectively 140.7 ° and 135.8 °, shows as hydrophobic
Property, belong to hydrophobic material.Diameter 2 μ l and 8 μ l water droplets become flat, Static Contact at ultrasonic rear fibroin nanofiber surface
Angle is respectively 9.7 ° and 4.0 °, shows as hydrophilic, belongs to hydrophilic material.Supersound process makes fibroin fiber that a point fibre to occur, and becomes
Obtain thinner, introduce great amount of hydroxy group at fiber surface simultaneously, promote fibroin fiber to become having excellent hydrophilic from hydrophobic fibre
Fibroin nanofiber, be effectively increased the biocompatibility of fibroin fiber.
Fibroin nanofiber prepared by embodiment 6 this method and fibroin fiber water vapo(u)r transmission can contrast
Medical dressing needs have suitable moisture-vapor transmission, i.e. possesses certain performance of keeping humidity and could maintain the " wet of wound
Profit environment ".Moisture-vapor transmission is the highest, is easily caused wound penetrating fluid and runs off and transition dehydration, and moisture-vapor transmission is the lowest, can shadow
Ring body eubolism and cause wound deterioration.When 35 DEG C, people's normal skin moisture evaporation amount is 240 ± 12 g m-2·
d-1, and injured skin moisture evaporation amount is relatively big, according to degree of injury, from 279 ± 26 g m-2·d-1To 5138 ± 202 g
m-2·d-1, the preferable moisture-vapor transmission of recommended medical dressing is at 2000～2500 g m-2·d-1Between.Attached
Figure 10 is the moisture-vapor transmission value of fibroin fiber and fibroin nanofiber, and result display fibroin fiber moisture-vapor transmission is average
Value is 1912.41 g m-2·d-1, the moisture-vapor transmission meansigma methods of fibroin nanofiber is 2133.74 g m-2·d-1。
The moisture-vapor transmission of fibroin nanofiber, in the range of preferable dressing, has than Bacterial cellulose (BC-Foam in accompanying drawing 11)
There is more preferable poisture-penetrability.
Fibroin nanofiber prepared by embodiment 7 this method contrasts with fibroin fiber medicament slow release performance
Selecting representational antibiotic amoxicillin is model drug, measures prepared nanofiber and delays the absorption of amoxicillin
Releasing performance, drug release patterns is as shown below, by accompanying drawing 12 it can be seen that fibroin nanofiber has preferably than fibroin fiber
Medicament slow release performance, its drug accumulation of 72 hours release percentage ratio is between 8%～16%.
Claims (4)
1. the method efficiently preparing fibroin nanofiber, it is characterised in that use convex battle array or linear array probe, ultrasonic probe and
All being immersed in solution by ultrasonic fibroin fiber, power is about between 200W ~ 1750W, and frequency is 5 KHz～5 megahertzs, super
The sound persistent period is 1～24 hour, and ultrasonic interval time stops 0～10s for surpassing 3～60s, during control by ultrasonic solution temperature
Degree is between 0 DEG C～90 DEG C；To be fixed on by ultrasonic fibroin fiber on the level clamp of square hollow or circular hollow, control
Ultrasonic probe is 0.1～5cm to the vertical dimension of fibroin fiber place plane, moves horizontally with the speed of 0～5cm/Min and is surpassed
The fixture at sound fibroin fiber place, thus prepare fibroin nano-fiber material.
2. the fibroin fiber described in claim 1 includes that silkworm, Antherea pernyi Guerin-Meneville, giant silkworm, Bombyx mandarina Moore and transgenic silkworm are weaved silk, cocoond
What case degumming post-treatment was made forms fiber-like material with fibroin albumen for main body.
3. the solution described in claim 1 refers to any one of weak acid, weak base, pure water and ultra-pure water or multiple, or
Person is any 2 in sodium carbonate, tertiary sodium phosphate, sodium dihydrogen phosphate and disodium hydrogen phosphate～3 kind of mixed solution.
4. fibroin nanofiber prepared by the present invention has good hydrophilic, water vapo(u)r transmission energy and medicament slow release performance,
This patent protects its application in terms of medical dressing.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114908433A (en) * | 2021-02-07 | 2022-08-16 | 浙江大学 | Method for preparing fibroin nano-fiber by physical method and product |
| CN115444818A (en) * | 2022-08-12 | 2022-12-09 | 青岛科技大学 | Eye drop solubilizing auxiliary material based on silk fibroin nanofiber and preparation method of eye drop medicament containing auxiliary material |
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| CN102605439A (en) * | 2012-02-24 | 2012-07-25 | 绍兴文理学院 | Silk fabric degumming method and apparatus thereof |
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| JPH04297411A (en) * | 1991-03-26 | 1992-10-21 | Kanebo Ltd | Silk pigment adsorbing colloidal titanium oxide and its production |
| JP2002363861A (en) * | 2001-06-05 | 2002-12-18 | Hiromoto Uejima | Method for carrying out silk fibroin processing of fiber or fiber product |
| CN1730734A (en) * | 2005-08-19 | 2006-02-08 | 清华大学 | Natural nanometer fiber preparation method |
| CN102605439A (en) * | 2012-02-24 | 2012-07-25 | 绍兴文理学院 | Silk fabric degumming method and apparatus thereof |
| CN102732978A (en) * | 2012-07-05 | 2012-10-17 | 绍兴文理学院 | Silk degumming process implemented by using ultrasonic waves |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114908433A (en) * | 2021-02-07 | 2022-08-16 | 浙江大学 | Method for preparing fibroin nano-fiber by physical method and product |
| CN115444818A (en) * | 2022-08-12 | 2022-12-09 | 青岛科技大学 | Eye drop solubilizing auxiliary material based on silk fibroin nanofiber and preparation method of eye drop medicament containing auxiliary material |
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