CN105861616A - Agrocybe Cylindracea liquid strain quality detection method - Google Patents
Agrocybe Cylindracea liquid strain quality detection method Download PDFInfo
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Abstract
The invention discloses an Agrocybe Cylindracea liquid strain quality detection method. The method comprises the following steps: taking an Agrocybe Cylindracea strain liquid, pouring the strain liquid into a screen mesh 1 with the mesh size of 0.6*0.6mm, and collecting a filtrate 1; pouring the filtrate 1 into a screen mesh 1 with the mesh size of 0.05*0.05mm, and collecting a filtrate 2, wherein the liquid of mycelium pellets with appropriate sizes meets the following conditions: the quantity of the mycelium pellets on the screen mesh 1 is smaller than 10, and the scale of a measuring cylinder can be observed behind the strain liquid in the filtrate 2; pouring the Agrocybe Cylindracea strain liquid into the measuring cylinder, and standing; and observing the distribution state of the mycelium pellets, and judging the liquid strains meeting that the volume of a liquid under the mycelium pellets is smaller than 20ml or the volume of a supernatant in the measuring cylinder is smaller than 20ml as liquid strains with qualified vitality. The method can rapidly and effectively examine the vitality and the quality of Agrocybe Cylindracea liquid strains, and prevents strain ageing induced by too long growth time of mycelia, or influences of insufficient strain vitality on subsequent production.
Description
Technical field
The present invention relates to liquid spawn detection method, be specifically related to agrocyb eaegerita liquid spawn quality determining method.
Background technology
Edible fungi liquid strain production technology has compared with solid culture that incubation time is short, fast, cell age of sending out bacterium
Neatly, inoculate the advantages such as convenient, reduce production cost simultaneously, be very suitable for being applied to as supporting technology
Factorial praluction, can shorten the later stage production of hybrid seeds and planting time accordingly.
The success or failure of the training quality of agrocyb eaegerita liquid spawn good bad influence inoculation and the growth promoter in later stage.Camellia sinensis
Mushroom pompon diameter directly affects the technological process of inoculation, the efficiency of mycelium pellet excessive impact inoculation, bacterium
Pompon is too small, after inoculation mycelia to send out the bacterium time longer, incubation time increases.Additionally the vigor of mycelium pellet is to sending out
Bacterium time, the mycelia speed of growth in bacterium bag have a significant effect.
Quality evaluation to agrocyb eaegerita liquid spawn at present is mainly with hypha biomass as leading indicator, to liquid
The vigor of body strain is difficult to judge.
Summary of the invention
It is an object of the invention to provide a kind of agrocyb eaegerita liquid spawn quality determining method.
The purpose of the present invention is achieved through the following technical solutions:
Agrocyb eaegerita liquid spawn quality determining method, comprises the following steps:
(1) mycelium pellet size detection
Take the agrocyb eaegerita bacterium solution of 100ml liquid culture, pour in the screen cloth 1 that mesh size is 0.6 × 0.6mm,
Collect filtered solution 1；Filtered solution 1 is poured into the screen cloth 2 that mesh size is 0.05 × 0.05mm, uses glass cylinder
Collect filtered solution 2；
The mesh size of screen cloth 1 is to produce supporting inoculating mechanism according to later stage agrocyb eaegerita to come fixed, and diameter is big
If the mycelium pellet number in this size will block the inoculating gun in later stage too much, impact produces.Screen cloth 2
Mesh size rule of thumb sum up and draw, in general diameter less than this number mycelium pellet vigor not
High.
If the mycelium pellet number on screen cloth 1 is more than 10, its number of mycelium pellet that in bacterium solution, diameter is excessive is described
Mesh is more, and this has harmful effect to post incoulation technique；If in filtered solution 2, become clear light conditions in indoor
Under, the scale of graduated cylinder, then its number of mycelium pellet that in explanation bacterium solution, diameter is too small cannot be observed through bacterium solution
More, send out the bacterium time after the inoculation of this bacterium solution longer；Therefore, both bacterium solution are not the most suitable bacterium solution；
The sizeable bacterium solution of mycelium pellet is: the mycelium pellet number on screen cloth 1 is less than 10, simultaneously at filtered solution 2
In, through bacterium solution it is observed that the scale of graduated cylinder；
(2) growth state-detection
Take mycelium pellet sizeable agrocyb eaegerita bacterium solution 100ml, pour in graduated cylinder, stand 0.5-1 hour；
Observe mycelium pellet distribution, if mycelium pellet sinks, observe the volume of supernatant in graduated cylinder, if supernatant
More than 20ml, liquid is long-pending then illustrates that mycelium pellet vigor is not enough；If mycelium pellet is floating, then observe graduated cylinder lower liquid,
If the following liquid volume of mycelium is more than 20ml, illustrate that mycelium pellet is aging；If mycelium pellet suspends is full of whole amount
Cylinder, then under this state, mycelia vigor is the highest；In graduated cylinder, the following liquid volume of mycelium pellet is less than 20ml, or amount
In Tong, the supernatant volume liquid spawn less than 20ml is that vigor is qualified, can be used for subsequent inoculations；
The described graduated cylinder that graduated cylinder preferable volume is 100ml, it is simple to observe scale and bacterium solution volume.
The present invention has such advantages as relative to prior art and effect:
1, the inventive method can judge the quality of liquid spawn exactly from 2 indexs by numerical values recited.
2, the inventive method detection is rapidly, it is not necessary to specific instrument and equipment, method is simple.
3, the inventive method can fast and effeciently check vigor and the quality of agrocyb eaegerita liquid spawn, prevents bacterium
The growth time of silk is long there is strain aging, or owing to incubation time is not enough, spawn activity shadow not
Ring follow-up production.This method is applicable to the quality of production of agrocyb eaegerita liquid spawn and controls, and improves production efficiency.
Accompanying drawing explanation
Fig. 1 is the big logotype of mycelium pellet in each batch agrocyb eaegerita bacterium solution.
Fig. 2 is mycelium pellet distribution figure in graduated cylinder in each batch agrocyb eaegerita bacterium solution.
Fig. 3 is the growth conditions figure that agrocyb eaegerita bacterium solution is inoculated on Semen Gossypii.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention
It is not limited to this.
Embodiment 1
Agrocyb eaegerita liquid spawn quality determining method, comprises the following steps:
(1) mycelium pellet size detection
The agrocyb eaegerita bacterium solution of the liquid culture of A batch, taking 100ml and pouring mesh size into is 0.6 × 0.6mm's
In screen cloth 1, collect filtered solution 1；
It is 12 by counting to get the mycelium pellet number on screen cloth 1, the mycelia that in bacterium solution, diameter is excessive is described
Its number of ball is more, and this has harmful effect to post incoulation technique.Without carrying out the detection of subsequent step again.
The mycelium pellet size of this batch bacterium solution is as shown in Figure 1A.
Embodiment 2
Agrocyb eaegerita liquid spawn quality determining method, comprises the following steps:
(1) mycelium pellet size detection
The agrocyb eaegerita bacterium solution of the liquid culture of B batch, taking 100ml and pouring mesh size into is 0.6 × 0.6mm's
In screen cloth 1, collect filtered solution 1；It it is 1 by counting to get the mycelium pellet number on screen cloth 1.
Filtered solution 1 is poured into the screen cloth 2 that mesh size is 0.05 × 0.05mm, receives with 100ml glass cylinder
Collection filtered solution 2；In filtered solution 2, in the case of indoor bright light is shone, graduated cylinder cannot be observed through bacterium solution
Scale, illustrate that its number of mycelium pellet that in this batch bacterium solution, diameter is too small is more, this bacterium solution inoculation after send out
The bacterium time is longer.Therefore, this batch bacterium solution is not suitable bacterium solution.
The mycelium pellet size of this batch bacterium solution is as shown in Figure 1B.
Embodiment 3
Agrocyb eaegerita liquid spawn quality determining method, comprises the following steps:
(1) mycelium pellet size detection
The agrocyb eaegerita bacterium solution of the liquid culture of C batch, taking 100ml and pouring mesh size into is 0.6 × 0.6mm's
In screen cloth 1, collect filtered solution 1；It it is 2 by counting to get the mycelium pellet number on screen cloth 1.
Filtered solution 1 is poured into the screen cloth 2 that mesh size is 0.05 × 0.05mm, receives with 100ml glass cylinder
Collection filtered solution 2；In filtered solution 2, indoor bright light according in the case of, through bacterium solution it is observed that graduated cylinder
Scale.Therefore, the bacterium solution of this batch is the sizeable bacterium solution of mycelium pellet.The mycelium pellet of this batch bacterium solution is big
Little as shown in Figure 1 C.
(2) growth state-detection
Take agrocyb eaegerita bacterium solution 100ml of C batch, pour in 100ml graduated cylinder, stand 0.5-1 hour；Observe
Mycelium pellet distribution, finds that mycelium pellet sinks, and in graduated cylinder, the volume of supernatant is more than 20ml, and this explanation should
In batch bacterium solution, mycelium pellet vigor is not enough.
The mycelium pellet of this batch bacterium solution distribution in graduated cylinder is as shown in Figure 2 A.
Embodiment 4
Agrocyb eaegerita liquid spawn quality determining method, comprises the following steps:
(1) mycelium pellet size detection
The agrocyb eaegerita bacterium solution of the liquid culture of D batch, taking 100ml and pouring mesh size into is 0.6 × 0.6mm's
In screen cloth 1, collect filtered solution 1；It it is 1 by counting to get the mycelium pellet number on screen cloth 1.
Filtered solution 1 is poured into the screen cloth 2 that mesh size is 0.05 × 0.05mm, receives with 100ml glass cylinder
Collection filtered solution 2；In filtered solution 2, indoor bright light according in the case of, through bacterium solution it is observed that graduated cylinder
Scale.Therefore, the bacterium solution of this batch is the sizeable bacterium solution of mycelium pellet.The mycelium pellet of this batch bacterium solution is big
The little approximation with C batch.
(2) growth state-detection
Take agrocyb eaegerita bacterium solution 100ml of D batch, pour in 100ml graduated cylinder, stand 0.5-1 hour；Observe
Mycelium pellet distribution, finds that in graduated cylinder, mycelium pellet is floating, and the following liquid volume of mycelium is more than 20ml, says
The mycelium pellet of this batch bacterium solution bright is aging, is not suitable for subsequent inoculations.
The mycelium pellet of this batch bacterium solution distribution in graduated cylinder is as shown in Figure 2 B.
If bacterium solution 30ml of this batch being inoculated in cotton seed hulls culture bag, through growth in 25 days, bacterium bag was still
Do not cover with agrocyb eaegerita mycelia, as shown in Fig. 3 A, 3B.
Embodiment 5
Agrocyb eaegerita liquid spawn quality determining method, comprises the following steps:
(1) mycelium pellet size detection
The agrocyb eaegerita bacterium solution of the liquid culture of E batch, taking 100ml and pouring mesh size into is 0.6 × 0.6mm's
In screen cloth 1, collect filtered solution 1；It it is 2 by counting to get the mycelium pellet number on screen cloth 1.
Filtered solution 1 is poured into the screen cloth 2 that mesh size is 0.05 × 0.05mm, receives with 100ml glass cylinder
Collection filtered solution 2；In filtered solution 2, indoor bright light according in the case of, through bacterium solution it is observed that graduated cylinder
Scale.Therefore, the bacterium solution of this batch is the sizeable bacterium solution of mycelium pellet.The mycelium pellet of this batch bacterium solution is big
The little approximation with C batch.
(2) growth state-detection
Take agrocyb eaegerita bacterium solution 100ml of E batch, pour in 100ml graduated cylinder, stand 0.5-1 hour；Observe
Mycelium pellet distribution, finds that mycelium pellet suspends and is full of whole graduated cylinder, under this state, mycelia vigor is the highest, explanation
The bacterium solution of this batch can be used for subsequent inoculations.
The mycelium pellet of this batch bacterium solution distribution in graduated cylinder is as shown in Figure 2 C.
On bacterium solution 30ml of this batch being inoculated in cotton seed hulls culture bag, through growth in 25 days, bacterium bag covered with
Agrocyb eaegerita mycelia, as shown in Fig. 3 C, 3D.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not by above-mentioned enforcement
The restriction of example, the change made, modifies, replaces under other any spirit without departing from the present invention and principle
In generation, combine, simplify, all should be the substitute mode of equivalence, within being included in protection scope of the present invention.
Claims (2)
1. agrocyb eaegerita liquid spawn quality determining method, it is characterised in that comprise the following steps:
(1) mycelium pellet size detection
Take the agrocyb eaegerita bacterium solution of 100ml liquid culture, pour in the screen cloth 1 that mesh size is 0.6 × 0.6mm,
Collect filtered solution 1；Filtered solution 1 is poured into the screen cloth 2 that mesh size is 0.05 × 0.05mm, uses glass cylinder
Collect filtered solution 2；
If the mycelium pellet number on screen cloth 1 is more than 10, its number of mycelium pellet that in bacterium solution, diameter is excessive is described
Mesh is more, and this has harmful effect to post incoulation technique；If in filtered solution 2, become clear light conditions in indoor
Under, the scale of graduated cylinder, then its number of mycelium pellet that in explanation bacterium solution, diameter is too small cannot be observed through bacterium solution
More, send out the bacterium time after the inoculation of this bacterium solution longer；
The sizeable bacterium solution of mycelium pellet is: the mycelium pellet number on screen cloth 1 is less than 10, is filtering simultaneously
In liquid 2, through bacterium solution it is observed that the scale of graduated cylinder；
(2) growth state-detection
Take mycelium pellet sizeable agrocyb eaegerita bacterium solution 100ml, pour in graduated cylinder, stand 0.5-1 hour；
Observe mycelium pellet distribution, if mycelium pellet sinks, observe the volume of supernatant, supernatant in graduated cylinder
More than 20ml, volume then illustrates that mycelium pellet vigor is not enough；If mycelium pellet is floating, then observe graduated cylinder lower liquid,
More than 20ml, the following liquid volume of mycelium then illustrates that mycelium pellet is aging；If mycelium pellet suspends is full of whole graduated cylinder,
Then under this state, mycelia vigor is the highest；In graduated cylinder, the following liquid volume of mycelium pellet is less than in 20ml, or graduated cylinder
The supernatant volume liquid spawn less than 20ml is that vigor is qualified, can be used for subsequent inoculations.
Agrocyb eaegerita liquid spawn quality determining method the most according to claim 1, it is characterised in that: institute
The graduated cylinder stated be volume be the graduated cylinder of 100ml.
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| CN201610187011.4A CN105861616A (en) | 2016-03-29 | 2016-03-29 | Agrocybe Cylindracea liquid strain quality detection method |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101550399A (en) * | 2008-04-02 | 2009-10-07 | 中国科学院沈阳应用生态研究所 | Resting preservation method of liquid strain in edible fungus |
| CN101779574A (en) * | 2010-03-17 | 2010-07-21 | 浙江大学 | Method for culturing of edible fungi |
| CN103875446A (en) * | 2013-10-24 | 2014-06-25 | 江西省农业科学院农业应用微生物研究所 | Method of producing Agrocybe aegerita strains by liquid culture and preservation method of Agrocybe aegerita strain |
| CN104988070A (en) * | 2015-06-11 | 2015-10-21 | 天津中天精科科技有限公司 | Pleurotus eryngii liquid strain with small and many mycelium pellets and fermentation method of pleurotus eryngii liquid strain |
-
2016
- 2016-03-29 CN CN201610187011.4A patent/CN105861616A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101550399A (en) * | 2008-04-02 | 2009-10-07 | 中国科学院沈阳应用生态研究所 | Resting preservation method of liquid strain in edible fungus |
| CN101779574A (en) * | 2010-03-17 | 2010-07-21 | 浙江大学 | Method for culturing of edible fungi |
| CN103875446A (en) * | 2013-10-24 | 2014-06-25 | 江西省农业科学院农业应用微生物研究所 | Method of producing Agrocybe aegerita strains by liquid culture and preservation method of Agrocybe aegerita strain |
| CN104988070A (en) * | 2015-06-11 | 2015-10-21 | 天津中天精科科技有限公司 | Pleurotus eryngii liquid strain with small and many mycelium pellets and fermentation method of pleurotus eryngii liquid strain |
Non-Patent Citations (3)
| Title |
|---|
| 张广田: "如何检测液体菌种的质量", 《农业知识（瓜果菜）》 * |
| 曾泽彬 等: "茶树菇发酵罐培养的工艺优化", 《食用菌》 * |
| 邵坤: "外源激素对茶树菇液体培养胞外酶活性影响的研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
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