CN105853465B - A method of inhibit vibrio parahaemolytious in mussel body to breed by lactic acid bacteria biological antagonism - Google Patents

A method of inhibit vibrio parahaemolytious in mussel body to breed by lactic acid bacteria biological antagonism Download PDF

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CN105853465B
CN105853465B CN201610182359.4A CN201610182359A CN105853465B CN 105853465 B CN105853465 B CN 105853465B CN 201610182359 A CN201610182359 A CN 201610182359A CN 105853465 B CN105853465 B CN 105853465B
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mussel
lactococcus lactis
cicc
vibrio parahaemolytious
lactis
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CN105853465A (en
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阮晖
胡慧雯
金迅
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Zhejiang University ZJU
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

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Abstract

The invention discloses a kind of methods for inhibiting vibrio parahaemolytious breeding in mussel body by lactic acid bacteria biological antagonism, comprising: cultivates Lactococcus lactis Lactococcus lactis subsp.Lactis CICC 6242, it is 10 that density, which is made,9~1010The high density bacteria suspension of cfu/mL；High density bacteria suspension is added in the water body of cultivation mussel, inhibits vibrio parahaemolytious breeding in shellfish body.In the present invention, a kind of harmless bacteriocin Nisin to bacterium with strong killing effect is generated by Lactococcus lactis Lactococcus lactis subsp.Lactis CICC 6242, it is able to suppress vibrio parahaemolytious in mussel body to breed, inhibits vibrio parahaemolytious breeding in mussel body to ensure mussel edible safety by biological antagonist.

Description

It is a kind of that vibrio parahaemolytious breeding in mussel body is inhibited by lactic acid bacteria biological antagonism Method
Technical field
The present invention relates to field of food safety, and in particular to one kind inhibits secondary molten in mussel body by lactic acid bacteria biological antagonism The method of blood vibrios breeding.
Background technique
Chinese nearly 1,000,000 tons of cultivation amount of mussel year, Zhejiang Province is mussel major production areas, 15% or more Zhan Quanguo total output. In the past 30 years, the factors such as global warming, vibrio parahaemolytious (Vibrio in seawater are added in CHINESE OFFSHORE pollution aggravation Parahaemolyticus, Vp) content is on the rise.The pathogenic influence factor of VP is more, such as invasiveness, hemotoxin, In again it is mostly important with Thermostable direc t hemolysin (Thermalstable Directheamolysin, TDH).The food of VP pollution Object can cause acute gastroenteritis, and patient symptom is mainly shown as diarrhea, vomiting, headache, nausea, cramp and low fever etc., disease Journey is generally 2~4d, and the duration is shorter, and can self-healing.Severe can also cause wound infection and septicemia, the other tissues of body Also lesion, such as skin bulla damage, adjuvant arthritis and rheumatoid arthritis can be generated.
And mussel is filter food, and Vp can be largely enriched with from environment, and mussel is made to become the important food source of Vp diarrhea. It is shown according to the monitoring data of country, China food origin disease monitoring net, food poisoning is in food posioning as caused by Vp In already take up critical positions.
Mussel is after fishing disembarkation, to keep its freshness, often continue to cultivate at normal temperature in sales section rather than It is refrigerated or is freezed.Vp will continue to breed at normal temperature.Therefore, it to ensure mussel edible safety, needs to develop at normal temperature Vp in mussel body is inhibited to breed and do not influence the technology of mussel meat.
Summary of the invention
Vibrio parahaemolytious (Vp) breeding in mussel body is inhibited to protect by lactic acid bacteria biological antagonism the present invention provides a kind of The method for hindering mussel edible safety, by the Lactococcus lactis Lactococcus lactis subsp.lactis that will produce Nisin (CICC 6242) is added in the water body of cultivation mussel, plays the inhibiting effect to Vp in mussel body, to ensure the edible peace of mussel Entirely.
A method of inhibit vibrio parahaemolytious in mussel body to breed by lactic acid bacteria biological antagonism, comprising the following steps:
1) Lactococcus lactis Lactococcus lactis subsp.Lactis CICC 6242 is cultivated, is made Density is 109~1010The high density bacteria suspension of cfu/mL；
2) by step 1) 109~1010The high density bacteria suspension of cfu/mL is added in the water body of cultivation mussel, inhibits shellfish Internal vibrio parahaemolytious breeding.
In the present invention, generated by Lactococcus lactis Lactococcus lactis subsp.Lactis (CICC 6242) Bacteriocin Nisin that is a kind of harmless and having strong killing effect to bacterium, is able to suppress vibrio parahaemolytious in mussel body (Vp) it breeds, Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) is inhibited by biological antagonist Vp breeds and ensures mussel edible safety in mussel body.
In step 1), Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) is using existing Technology is come from Chinese industrial Microbiological Culture Collection administrative center (CICC), address: Chaoyang District, Beijing City using commercial product No. 6 building of the institute of winebibber's bridge Road 24；The deposit date is 2010, it was 6242 that strain, which keeps number,.
Preferably, Lactococcus lactis Lactococcus lactis subsp.Lactis CICC 6242 is trained It supports, comprising: the density of setting out of Lactococcus lactis Lactococcus lactis subsp.Lactis CICC 6242 is 106~ 107A/mL, being seeded to inoculum concentration in fluid nutrient medium is 1%~5%, 33 DEG C~39 DEG C 20~28h of shaken cultivation, until bacterium solution Density is 109~1010The high density bacteria suspension of cfu/mL.
Further preferably, Lactococcus lactis Lactococcus lactis subsp.Lactis CICC 6242 is carried out Culture, comprising: the density of setting out of Lactococcus lactis Lactococcus lactis subsp.Lactis CICC 6242 is 106 ~107A/mL, being seeded to inoculum concentration in fluid nutrient medium is 2%~3%, 36 DEG C~37 DEG C 22~26h of shaken cultivation, until bacterium Liquid density is 109~1010The high density bacteria suspension of cfu/mL.
The fluid nutrient medium, in terms of 1L, including following components:
The pH value of the fluid nutrient medium is adjusted to 6.0~6.6.
Further preferably, the fluid nutrient medium, in terms of 1L, including following components:
The pH value of the fluid nutrient medium is adjusted to 6.3, and fluid nutrient medium is in 121 DEG C of sterilizing 20min.
In step 2), preferably, high density bacteria suspension is added in the water body of room temperature cultivation mussel, it is according to compared to water body 1~3% ratio is added.I.e. the volume ratio of high density bacteria suspension and the water body of cultivation mussel is 1~3:100, further preferably, High density bacteria suspension is added in the water body of room temperature cultivation mussel, is added according to the ratio for being 2% compared to water body.I.e. high density bacterium is outstanding The volume ratio of liquid and the water body of cultivation mussel is 2:100.
After high density bacteria suspension is added in the water body of room temperature cultivation mussel, Lactococcus lactis Lactococcus Lactis subsp.lactis (CICC 6242) continues natural propagation to play the inhibition to vibrio parahaemolytious in mussel body and make With.
Compared with prior art, the present invention has the advantage that
In the present invention, generated by Lactococcus lactis Lactococcus lactis subsp.Lactis (CICC 6242) Bacteriocin Nisin that is a kind of harmless and having strong killing effect to bacterium, is able to suppress vibrio parahaemolytious in mussel body (Vp) it breeds, Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) is inhibited by biological antagonist Vp breeds and ensures mussel edible safety in mussel body, is conducive to market-oriented utilization and extention, has wide application prospect.
Specific embodiment
Embodiment 1
1) Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) high density of Nisin is produced The density of setting out of culture, CICC 6242 is 1.10 × 106A/mL, be seeded to fluid nutrient medium (in terms of 1L, peptone 10.0g, Beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, glucose 20.0g, sodium acetate 5.0g, dipotassium hydrogen phosphate 2.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, Tween 80 1.0mL, water surplus；PH=6.3,121 DEG C of sterilizing 20min) in, it connects Kind amount is 1%, and for 24 hours, bacterium solution density is 2.25 × 10 to 37 DEG C of shaken cultivations9Cfu/mL obtains high density bacteria suspension.
2) by Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) density be 2.25 × 109The high density bacteria suspension of cfu/mL is added in the water body of 25 DEG C of room temperature cultivation mussels according to the ratio for being 1% compared to water body, In 25 DEG C of water bodys of room temperature, Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) passes through biology Antagonism inhibits vibrio parahaemolytious breeding in mussel body and ensures mussel edible safety.
3) after the processing of step 1) and step 2) (for 24 hours after r), Vp density is obviously inhibited in mussel, specifically See Table 1 for details.
Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) comes from the micro- life of Chinese industrial Object culture presevation administrative center (CICC), it is 6242 that strain, which keeps number,.
Vp is measured in mussel: according to most may be used for standard SN 0173-2010 " the export food vibrio parahemolyticus method of inspection " It can number (most probable numer, MPN) 9 tube method.
Table 1
Embodiment 2
1) Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) high density of Nisin is produced The density of setting out of culture, CICC 6242 is 1.23 × 106A/mL, be seeded to fluid nutrient medium (in terms of 1L, peptone 10.0g, Beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, glucose 20.0g, sodium acetate 5.0g, dipotassium hydrogen phosphate 2.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, Tween 80 1.0mL, water surplus；PH=6.3,121 DEG C of sterilizing 20min) in, it connects Kind amount is 5%, and for 24 hours, bacterium solution density is 2.63 × 10 to 37 DEG C of shaken cultivations9Cfu/mL obtains high density bacteria suspension.
2) by Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) density be 2.63 × 109The high density bacteria suspension of cfu/mL is added in the water body of 25 DEG C of room temperature cultivation mussels according to the ratio for being 3% compared to water body, In 25 DEG C of water bodys of room temperature, Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) passes through biology Antagonism inhibits vibrio parahaemolytious breeding in mussel body and ensures mussel edible safety.
3) after the processing of step 1) and step 2) (for 24 hours after r), Vp density is obviously inhibited in mussel, specifically See Table 2 for details.
Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) comes from the micro- life of Chinese industrial Object culture presevation administrative center (CICC), it is 6242 that strain, which keeps number,.
Vp is measured in mussel: according to most may be used for standard SN 0173-2010 " the export food vibrio parahemolyticus method of inspection " It can number (most probable numer, MPN) 9 tube method.
Table 2
Embodiment 3
1) Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) high density of Nisin is produced The density of setting out of culture, CICC 6242 is 1.34 × 106A/mL is seeded to fluid nutrient medium (peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, glucose 20.0g, sodium acetate 5.0g, dipotassium hydrogen phosphate 2.0g, sulfuric acid Magnesium 0.58g, manganese sulfate 0.25g, Tween 80 1.0mL, water surplus；PH=6.3,121 DEG C of sterilizing 20min) in, inoculum concentration is 2%, for 24 hours, bacterium solution density is 2.52 × 10 to 37 DEG C of shaken cultivations9Cfu/mL obtains high density bacteria suspension.
2) by Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) density be 2.52 × 109The high density bacteria suspension of cfu/mL is added in the water body of 25 DEG C of room temperature cultivation mussels according to the ratio for being 2% compared to water body, In 25 DEG C of water bodys of room temperature, Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) passes through biology Antagonism inhibits vibrio parahaemolytious breeding in mussel body and ensures mussel edible safety.
3) after the processing of step 1) and step 2) (for 24 hours after r), Vp density is obviously inhibited in mussel, specifically See Table 3 for details.
Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) comes from the micro- life of Chinese industrial Object culture presevation administrative center (CICC), it is 6242 that strain, which keeps number,.
Vp is measured in mussel: according to most may be used for standard SN 0173-2010 " the export food vibrio parahemolyticus method of inspection " It can number (most probable numer, MPN) 9 tube method.
Table 3
Embodiment 4
1) Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) high density of Nisin is produced The density of setting out of culture, CICC 6242 is 1.07 × 106A/mL is seeded to fluid nutrient medium (peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, glucose 20.0g, sodium acetate 5.0g, dipotassium hydrogen phosphate 2.0g, sulfuric acid Magnesium 0.58g, manganese sulfate 0.25g, Tween 80 1.0mL, water surplus；PH=6.3,121 DEG C of sterilizing 20min) in, inoculum concentration is 3%, for 24 hours, bacterium solution density is 2.55 × 10 to 37 DEG C of shaken cultivations9Cfu/mL obtains high density bacteria suspension.
2) by Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) density be 2.55 × 109The high density bacteria suspension of cfu/mL is added in the water body of 25 DEG C of room temperature cultivation mussels according to the ratio for being 2% compared to water body, In 25 DEG C of water bodys of room temperature, Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) passes through biology Antagonism inhibits vibrio parahaemolytious breeding in mussel body and ensures mussel edible safety.
3) after the processing of step 1) and step 2) (for 24 hours after r), Vp density is obviously inhibited in mussel, specifically See Table 4 for details.
Lactococcus lactis Lactococcus lactis subsp.lactis (CICC 6242) comes from the micro- life of Chinese industrial Object culture presevation administrative center (CICC), it is 6242 that strain, which keeps number,.
Vp is measured in mussel: according to most may be used for standard SN 0173-2010 " the export food vibrio parahemolyticus method of inspection " It can number (most probable numer, MPN) 9 tube method.
Table 4

Claims (3)

1. a kind of method for inhibiting vibrio parahaemolytious breeding in mussel body by lactic acid bacteria biological antagonism, which is characterized in that including Following steps:
1) Lactococcus lactis (Lactococcus lactis subsp.Lactis) CICC 6242 is cultivated, density is made It is 109~1010The high density bacteria suspension of cfu/mL；
Wherein, the density of setting out of Lactococcus lactis (Lactococcus lactis subsp.Lactis) CICC 6242 is 106~ 107A/mL, being seeded to inoculum concentration in fluid nutrient medium is 2%~3%, 36 DEG C~37 DEG C 22~26h of shaken cultivation, until bacterium solution Density is 109~1010The high density bacteria suspension of cfu/mL；
The fluid nutrient medium, in terms of 1L, including following components:
The pH value of the fluid nutrient medium is adjusted to 6.0~6.6.
2) by step 1) 109~1010The high density bacteria suspension of cfu/mL is added in the water body of cultivation mussel, is inhibited in shellfish body Vibrio parahaemolytious breeding；The volume ratio of the water body of the high density bacteria suspension and cultivation mussel is 1~3:100.
2. the method according to claim 1 for inhibiting vibrio parahaemolytious breeding in mussel body by lactic acid bacteria biological antagonism, The fluid nutrient medium, in terms of 1L, including following components:
The pH value of the fluid nutrient medium is adjusted to 6.3, and the fluid nutrient medium is in 121 DEG C of sterilizing 20min.
3. the method according to claim 1 for inhibiting vibrio parahaemolytious breeding in mussel body by lactic acid bacteria biological antagonism, In step 2), the volume ratio of the water body of the high density bacteria suspension and cultivation mussel is 2:100.
CN201610182359.4A 2016-03-25 2016-03-25 A method of inhibit vibrio parahaemolytious in mussel body to breed by lactic acid bacteria biological antagonism Expired - Fee Related CN105853465B (en)

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CN102150925A (en) * 2011-01-10 2011-08-17 扬州大学 Bacteriostat prepared by fermentation of lactobacillus and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102150925A (en) * 2011-01-10 2011-08-17 扬州大学 Bacteriostat prepared by fermentation of lactobacillus and preparation method thereof

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* Cited by examiner, † Cited by third party
Title
Isolation and preliminary characterization of bacteriocins;S.D. Shehane等;《Journal of Applied Microbiology》;20020121;第92卷;第322–328页
固定化细胞发酵生产乳链菌肽工艺研究;陶静等;《中国食品添加剂》;20140730;第3卷;第158-162页，参见第159页左栏第2段-右栏第2段，第160页左栏第1段、图2
水产源乳酸菌的多样性及抑菌活性研究;刘君等;《水产科学》;20150630;第34卷(第6期);第351-357页，参见参见摘要、表2

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