CN105820215A - Method for efficient synthesis of Stylissatin A natural cyclo-heptapeptide - Google Patents
Method for efficient synthesis of Stylissatin A natural cyclo-heptapeptide Download PDFInfo
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Abstract
The invention relates to a method for efficient synthesis of Stylissatin A natural cyclo-heptapeptide. The method mainly comprises the steps of 1, analysis of a Stylissatin A natural cyclo-heptapeptide retrosynthesis route; 2, solid-phase synthesis of linear chain peptide 6; 3, cyclization of linear chain peptide 6; 4, efficient liquid-phase separation of the product cyclopeptide. The method has the advantages that synthesis is easy and yield is high. The method can overcome the defect of small natural extraction amount and provides a material basis for further activity study.
Description
Technical field
The present invention relates to compou nd synthesis technical field, specifically, be a kind of preparation method efficiently synthesizing StylissatinA native annulus heptapeptide.
Background technology
Naturally occurring cyclic peptide, due to the chemical constitution of its uniqueness and strong biological activity, has the highest patent medicine and is worth.They are not as linear peptides, and cyclic peptide is the most stable, have more preferable liposoluble and membrane permeability, and target spot illustrates higher specificity.These synthesis of cyclic peptides can be as promising drug candidate.Recently, the cyclic peptide of a series of proline rich is separated from marine organisms, such as rollamides, axinastatins, phakellistatins etc..But the content of the above-mentioned cyclic peptide separated from marine organisms is the lowest, in order to further study its biological activity and structure activity relationship, many researcheres concentrate on the synthesis of cyclic peptide, particularly solid phase synthesis process.StylissatinA is the native annulus heptapeptide of a kind of proline rich, nitric oxide production generation can be suppressed, it is a kind of natural product extracted from Papua New Guinea sponge, it can suppress the nitric oxide production generation of RAW264.7 cell under lipopolysaccharide stimulation, its half suppression ratio is 87uM, shows good biological activity.This ring seven peptide isolated from sponge in existing technology, but the StylissatinA content extracted from sponge is the lowest, is only capable of extracting 3mg target compound in 840g sponge material.Therefore develop the synthetic method of a kind of efficient solid phase synthesis StylissatinA native annulus heptapeptide, in order to make up due to the few defect of natural extract amount, provide material base for further activity research, and about such method, yet there are no report.
Summary of the invention
It is an object of the invention to for deficiency of the prior art, it is provided that a kind of preparation method efficiently synthesizing StylissatinA native annulus heptapeptide.
For achieving the above object, the present invention adopts the technical scheme that:
A kind of preparation method efficiently synthesizing StylissatinA native annulus heptapeptide, comprises the steps:
The first step:
1) compound 2 and Fmoc-Leu-OH reaction under the effect of N, N-diisopropylethylamine obtain compound 3；
2) compound 3 removes Fmoc protection group and obtains compound 4；
3) Fmoc-Pro-OH is coupled on compound 4 under I-hydroxybenzotriazole, DIPEA effect, then removing Fmoc protection group；
4) repeat step 3) successively Fmoc-Ile-OH, Fmoc-Pro-OH, Fmoc-Phe-OH, Fmoc-Tyr (tBu)-OH, Fmoc-Ile-OH are coupled to step 3) and product on, obtain chemical combination 5；
5) during compound 5 is placed in acetic acid/trifluoroethanol/dichloromethane mixed solution, reaction obtains compound 6；
Second step:
6) compound 6 hexafluorophosphoric acid benzotriazole-1-base-epoxide tripyrrole alkyl phosphorus, 1-hydroxy benzo triazole, N, N-diisopropylethylamine effect under reaction obtain compound 7；
7) during compound 7 is placed in trifluoroacetic acid/phenol/water/diisopropylsilyl mixed solution, reaction obtains target compound 1；
。
Preferably, described compound 2 is 2 chlorine trityl resin, and substitution value is 0.33mmol/g.Bead represents resin.
Preferably, in described step 1, the ratio of compound 2 and Fmoc-Leu-OH is 500mg:0.2mmol.
Preferably, in described step 2, compound 3 is placed in piperidine solution removing Fmoc protection group.
Preferably, in described step 3, Fmoc-Pro-OH, I-hydroxybenzotriazole, the ratio of DIPEA are 1mmol:365mg:365ul.
Preferably, acetic acid in described step 5, trifluoroethanol, methylene chloride volume are than for 1:2:16.
Preferably, in described step 6, hexafluorophosphoric acid benzotriazole-1-base-epoxide tripyrrole alkyl phosphorus, hydroxy benzo triazole, the mol ratio of DIPEA are 1:1:2.
Preferably, in described step 7, trifluoroacetic acid, phenol, water, diisopropylsilyl volume ratio are 88:5:5:2.
The described first step specifically comprises the following steps that
1) take 500mg2 chlorine trityl resin, clean each 5 times with dimethylformamide, dichloromethane respectively, be subsequently adding dichloromethane and soak resin 15min；
2) mixed solution of the Fmoc-Phe-OH of 0.2mmol, dichloromethane and dimethylformamide is taken, it is sufficiently mixed dissolving, it is subsequently adding the N of 360ul, N-diisopropylethylamine, joins in the resin of step 2 after dissolving, react 1h in 35 DEG C of gas bath isothermal vibration shaking tables, take out reactant liquor, clean with dimethylformamide, dichloromethane respectively, close resin with methanol, Yong dimethylformamide, dichloromethane, dimethylformamide cleaning resin；
3) adding the newly configured piperidine solution of 8ml20% in resin, first static 5min, then takes out, the piperidines adding 8ml20% is placed in constant temperature oscillator, concussion reaction 10min, takes out solution, cleans resin with dichloromethane, dimethylformamide respectively；
4) 1mmolFmoc-Pro-OH is taken, 365mg1-hydroxybenzotriazole, 8ml dimethylformamide, it is sufficiently mixed dissolving, add 365ulN, N-diisopropylethylamine, shake activation 1min, it is then added in the resin of step 3 concussion reaction 1h, take out reactant liquor, respectively with dimethylformamide, dichloromethane, dimethylformamide cleans resin, the newly configured piperidine solution of 8ml20% is added in resin, first static 5min, then take out, the piperidines adding 8ml20% is placed in constant temperature oscillator, concussion reaction 10min, solution is taken out, respectively with dichloromethane, dimethylformamide cleans resin；
5) method repeating step 4, is coupled to Fmoc-Ile-OH, Fmoc-Pro-OH, Fmoc-Phe-OH, Fmoc-Tyr (tBu)-OH, Fmoc-Ile-OH on resin successively；
6) by acetic acid: trifluoroethanol: the mixed solution 15ml of dichloromethane=1:2:16 adds in the resin of step 5,35 DEG C of constant temperature oscillation reaction 2-4h, obtain linear peptides.
Described second step specifically comprises the following steps that
1) 0.5mmol hexafluorophosphoric acid benzotriazole-1-base-epoxide tripyrrole alkyl phosphorus, 0.5mmol1-hydroxy benzo triazole, 1mmolN are taken, N-diisopropylethylamine, join in dichloromethane, N-Methyl pyrrolidone, ultrasonic dissolution, add 150ml anhydrous methylene chloride, prepare mixed solution, be placed in 0 DEG C；Taking 0.1mmol linear peptides anhydrous methylene chloride to dissolve, making linear peptides concentration is 0.5mg/ml, is slowly dropped in mixed solution by linear peptides solution, stirring reaction, obtains white solid powder shape product；
2) white solid powder shape product is joined (trifluoroacetic acid/phenol/water/diisopropylsilyl=88/5/5/2) mixed solution of 10ml, reacts 3 hours in 35 DEG C of isothermal vibrations, obtain target compound.
Described preparation method also includes the 3rd step:
After the mixed solution of the target product cyclic peptide acetonitrile obtained and water is dissolved, separate by efficient liquid phase (HPLC).Separation condition is as follows:
The component that will be separated, is placed in 50ml centrifuge tube with liquid nitrogen flash freezer, is subsequently placed in lyophilization in lyophilizer, obtains white solid powder.
The invention has the advantages that:
The present invention passes through experimental exploration, optimized choice is using phenylalanine and isoleucine as cyclization site, with isoleucine as the aminoterminal of linear peptides, using phenylalanine as the c-terminus of linear peptides, preparing StylissatinA native annulus heptapeptide, cyclic peptide prepared by the present invention by solid phase synthesis, cyclization, building-up process is simple, yield is high, can meet the bioactive test of target compound.
Accompanying drawing explanation
Accompanying drawing 1 is high-efficient liquid phase chromatogram (a) and high resolution mass spectrum figure (b) of target compound.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is expanded on further.Should be understood that these embodiments are merely to illustrate the present invention rather than limit the scope of the present invention.In addition, it is to be understood that after having read the content that the present invention records, the present invention can be made various changes or modifications by those skilled in the art, and these equivalent form of values fall within the application appended claims limited range equally.
The anti-synthetic route analysis of embodiment 1StylissatinA native annulus heptapeptide
StylissatinA is that the ring seven peptide of a kind of proline rich is with 4 kinds of different aminoacid.We are explored by pre-stage test, and optimized choice is using phenylalanine and isoleucine as cyclization site, using isoleucine as the aminoterminal of linear peptides, using phenylalanine as the c-terminus of linear peptides.Linear peptides is synthesized by solid phase synthesis means, carries out cyclization in linear peptides cancer liquid phase.Final thick peptide prod carries out isolated and purified with preparing liquid phase, it is thus achieved that final target compound.The synthesis strategy of StylissatinA.
The solid phase synthesis process of embodiment 2 linear peptides 6
Synthetic route as follows is used to prepare linear peptides compound 6.Wherein compound 2 represents 2 chlorine trityl resin
A.Fmoc-Leu-OH, dimethylformamide, dichloromethane, DIPEA, 1h；B.20% pyridine is in dimethylformamide, 15 minutes；C.Fmoc-AA-OH, I-hydroxybenzotriazole, DIPEA, dimethylformamide, 1h；D. acetic acid/trifluoroethanol/dichloromethane (1:2:16), 3h.
Specifically comprise the following steps that
(1) 500mg2 chlorine trityl resin (substitution value is 0.33mmol/g) is weighed, it is placed in dry solid phase synthesis pipe, resin is cleaned each 5 times respectively with dimethylformamide (DMF), dichloromethane (DCM), it is subsequently adding dichloromethane and soaks resin 15min, to make resin expansion open, be conducive to the carrying out of reaction.
(2) dichloromethane is then taken out, take the Fmoc-Phe-OH of 0.2mmol, it is placed in 10ml centrifuge tube, add the mixed solution (dimethylformamide: dichloromethane=2:8) of dichloromethane and dimethylformamide, aminoacid in test tube is shaken up dissolving, it is subsequently adding the N of 360ul, N-diisopropylethylamine, firmly shake up, it is subsequently adding in solid phase synthesis pipe, it is placed in (35 DEG C) in gas bath isothermal vibration shaking table, constant temperature oscillation reaction 1h, after reaction terminates, take out the liquid in composite tube, with dimethylformamide, dichloromethane is respectively washed 5 times, resin 15min is closed with methanol, after closing terminates, with dichloromethane, dimethylformamide respectively cleans resin 5 times.
(3) in solid phase synthesis pipe, add the newly configured piperidine solution of 8ml20%, first static 5min, then take out, the piperidines adding 8ml20% is placed in constant temperature oscillator, concussion reaction 10min, solution is taken out, is respectively washed resin 5 times with dichloromethane, dimethylformamide, dichloromethane respectively.From solid phase synthesis pipe, a small amount of resin is taken with capillary tube, it is placed in 2.5mlEP pipe, add configured in advance good 5% ethanol solution of ninhydrin 2, and the phenol ethanol solution 1 of 80%, being placed in boiling water bath and boil 3min, solution turned blue (if solution becomes blue explanation aminoacid and reacts on resin, has been connected on resin, if unchanged blue explanation unreacted, test need to be re-started).
(4) 1mmolFmoc-Pro-OH is taken, 365mg1-hydroxybenzotriazole is placed in 10ml centrifuge tube, adds 8ml dimethylformamide, fully shakes and make its solvent, it is subsequently adding 365ulN, N-diisopropylethylamine, fully shakes activation 1min, is subsequently adding in solid phase synthesis pipe, it is placed in gas bath constant-temperature table, concussion reaction 1h, takes out reactant liquor with water circulating pump, is respectively washed resin 5 times with dimethylformamide, dichloromethane, dimethylformamide respectively.nullThen it is placed in 2.5mlEP pipe with a little resin of capillary tube picking，Add configured in advance good 5% ethanol solution of ninhydrin 2，And the phenol ethanol solution 1 of 80%，It is placed in boiling water bath and boils 3min，If (solution becomes blue explanation aminoacid and reacts not exclusively or unreacted，Then need again to add Fmoc-Pro-OH to react once，Until detection does not has blue appearance，If unchanged blue explanation reaction the most completely)，Detection finds that solution is unchanged as blueness，The piperidine solution being pre-configured with of 8ml20% is added in solid phase synthesis pipe，First static 5min，Then take out，The piperidines adding 8ml20% is placed in constant temperature oscillator，Concussion reaction 10min，Solution is taken out，Use dichloromethane respectively、Dimethylformamide、Dichloromethane is respectively washed resin 5 times.
(5) according to the reaction method of the Fmoc-Pro-OH in step 4, successively by Fmoc-Ile-OH, Fmoc-Pro-OH, Fmoc-Phe-OH, Fmoc-Tyr (tBu)-OH, Fmoc-Ile-OH is assembled on resin, after Fmoc-AA-OH (wherein AA represents different aminoacid) reaction terminates, the newly configured piperidine solution of 8ml20% is added in solid phase synthesis pipe, first static 5min, then take out, the piperidines adding 8ml20% is placed in constant temperature oscillator, concussion reaction 10min, solution is taken out, respectively with dichloromethane, dimethylformamide, dichloromethane is respectively washed resin 5 times, resin is drained, treat that next portion reacts.
(6) by acetic acid that configured in advance is good: trifluoroethanol: the mixed solution 15ml of dichloromethane=1:2:16 adds in above-mentioned solid phase reaction tube, it is placed in gas bath constant temperature oscillator (35 DEG C), reaction 2-4h, reactant liquor pressurizing ball is extruded, it is placed in 50ml round-bottomed flask, rotary evaporation falls liquid therein, owing to acetic acid boiling point is higher, rotation needs to steam with toluene azeotropic during steaming, then rotation is steamed the oily liquids terminated to be placed in freeze dryer overnight, removed all of acetic acid, so as not under the influence of walk cyclization process.Take the sample after lyophilizing, determine molecular weight by Low Resolution Mass Spectra (ESI-MS).The gross production rate of final linear peptides synthesis is 67.5%, is greatly improved compared to liquid phase method productivity.
The cyclization of embodiment 3 linear peptides 6
Use synthetic route as follows to carry out cyclization and prepare target compound.
A. hexafluorophosphoric acid benzotriazole-1-base-epoxide tripyrrole alkyl phosphorus, hydroxy benzo triazole, DIPEA, N-Methyl pyrrolidone, dichloromethane, 0 DEG C. overnight；B. trifluoroacetic acid/phenol/water/tri isopropyl silane (88:5:5:2, v/v) stirring reaction 3 hours.
Specifically comprise the following steps that
null(1) hexafluorophosphoric acid benzotriazole-1-base-epoxide tripyrrole alkyl phosphorus 260mg (0.5mmol) is weighed，1-hydroxy benzo triazole 68mg (0.5mmol)，N,N-diisopropylethylamine 172ul (1mmol)，Add in the round-bottomed flask of 500ml，It is subsequently adding a little dichloromethane，Add 1-2mlN-methyl pyrrolidone，Ultrasonic dissolution sample，Then in round-bottomed flask, add 150ml anhydrous methylene chloride，It is placed in the cold well being cooled to 0 DEG C in advance，Stirring about 10min，Solution temperature is made to drop to 0 DEG C，Then the linear peptides 113.9mg (0.1mmol) that above-mentioned process completes is taken，Dissolve with 150ml anhydrous methylene chloride，The concentration of guarantee polypeptide is 0.5mg/ml，Prevent solubility excessive polypeptide generation dimerization or poly，In round-bottomed flask, polypeptide solution is dripped slowly with Dropping funnel，Place reaction liquid into after dropping and be stirred overnight under room temperature，Reactant liquor rotation is steamed by next day，Obtain grease，Dissolve with a little methanol，With methanol as washing and dehydrating integrated machine，Product cyclic peptide is isolated with SephadexLH-20 pillar，Component obtained by collection carries out rotation and steams，The oily sample that gained is steamed in rotation is dissolved with a little dichloromethane，It is subsequently adding PE rotation steaming to solidify，2-3 time repeatedly，Until obtaining white solid powder shape product，The cyclization yield being computed linear peptides is 66.4%.
(2) by the white solid product of above-mentioned gained, it is placed in the middle of 50ml round-bottomed flask, add 10ml (trifluoroacetic acid/phenol/water/diisopropylsilyl=88/5/5/2) mixed solution of fresh configuration, it is placed in (35 DEG C) in gas bath isothermal vibration device, concussion reaction 3 hours, after reaction terminates, reactant liquor is transferred in 50ml centrifuge tube, with bubbling argon, trifluoroacetic acid is blown off, then in 10ml centrifuge tube, add the iced absolute ether 35ml cooled down in advance shake even with this precipitation ring peptide prod, then 3500r/min, centrifugal 3min, outwell supernatant, then iced absolute ether is added, repeatedly for three times.Precipitated product is placed in fume hood, in order to ether volatilizes.Cyclic peptide crude product is i.e. obtained after sample volatilizes.
The separation of embodiment 4 product cyclic peptide
After cyclic peptide acetonitrile embodiment 3 prepared and the mixed solution of water dissolve, separate by efficient liquid phase (HPLC).Separation condition is as follows:
The component that will be separated, is placed in 50ml centrifuge tube with liquid nitrogen flash freezer, is subsequently placed in lyophilization in lyophilizer, obtains white solid powder, is 45% through calculating the final total recovery obtaining pure product, it is thus achieved that products collection efficiency high.
The discriminating of embodiment 5 product and structural analysis
The solid product powder of gained is prepared by embodiment 4, is carried out the discriminating of molecular weight by high resolution mass spectrum, and carry out structure elucidation by proton nmr spectra and carbon spectrum.Chromatographiccondition is: C18 post (enlightening horse, 4.6 × 250mm) in 20 minutes B pump by 70-10, flow velocity: 1mL/min, 25 DEG C；A pump: acetonitrile adds 0.1% trifluoroacetic acid；B pump: water adds 0.1% trifluoroacetic acid. high resolution mass spectrum: compound StylissatinA (molecular formula: C49H63N7O8), test value [M+H]+: 878.4817；Test value [M+Na]+: 900.4633.Result is as shown in Figure 1.
Ultimate yield is affected by embodiment 6 differential responses condition
The differential responses condition of embodiment 3 is optimized, it is as shown in the table that contrast is specifically set.
The above is only the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, on the premise of without departing from the inventive method; can also make some improvement and supplement, these improve and supplement and also should be regarded as protection scope of the present invention.
Claims (10)
1. the preparation method efficiently synthesizing StylissatinA native annulus heptapeptide, it is characterised in that comprise the steps:
The first step:
1) compound 2 and Fmoc-Leu-OH reaction under the effect of N, N-diisopropylethylamine obtain compound 3；
2) compound 3 removes Fmoc protection group and obtains compound 4；
3) Fmoc-Pro-OH is coupled on compound 4 under I-hydroxybenzotriazole, DIPEA effect, then removing Fmoc protection group；
4) repeat step 3) successively Fmoc-Ile-OH, Fmoc-Pro-OH, Fmoc-Phe-OH, Fmoc-Tyr (tBu)-OH, Fmoc-Ile-OH are coupled to step 3) and product on, obtain chemical combination 5；
5) during compound 5 is placed in acetic acid/trifluoroethanol/dichloromethane mixed solution, reaction obtains compound 6；
Second step:
6) compound 6 hexafluorophosphoric acid benzotriazole-1-base-epoxide tripyrrole alkyl phosphorus, 1-hydroxy benzo triazole, N, N-diisopropylethylamine effect under reaction obtain compound 7；
7) during compound 7 is placed in trifluoroacetic acid/phenol/water/diisopropylsilyl mixed solution, reaction obtains target compound 1；
。
The most according to claim 1, efficiently synthesize the preparation method of StylissatinA native annulus heptapeptide, it is characterised in that described compound 2 is 2 chlorine trityl resin, substitution value is 0.33mmol/g.
Efficiently synthesize the preparation method of StylissatinA native annulus heptapeptide the most according to claim 1, it is characterised in that in described step 1, compound 2 is 500mg:0.2mmol with the ratio of Fmoc-Leu-OH.
Efficiently synthesize the preparation method of StylissatinA native annulus heptapeptide the most according to claim 1, it is characterised in that in described step 2, compound 3 is placed in piperidine solution removing Fmoc protection group.
Efficiently synthesize the preparation method of StylissatinA native annulus heptapeptide the most according to claim 1, it is characterised in that in described step 3, Fmoc-Pro-OH, I-hydroxybenzotriazole, the ratio of DIPEA are 1mmol:365mg:365ul.
Efficiently synthesize the preparation method of StylissatinA native annulus heptapeptide the most according to claim 1, it is characterised in that acetic acid in described step 5, trifluoroethanol, methylene chloride volume are than for 1:2:16.
Efficiently synthesize the preparation method of StylissatinA native annulus heptapeptide the most according to claim 1, it is characterized in that, in described step 6, hexafluorophosphoric acid benzotriazole-1-base-epoxide tripyrrole alkyl phosphorus, hydroxy benzo triazole, the mol ratio of DIPEA are 1:1:2.
Efficiently synthesize the preparation method of StylissatinA native annulus heptapeptide the most according to claim 1, it is characterised in that in described step 7, trifluoroacetic acid, phenol, water, diisopropylsilyl volume ratio are 88:5:5:2.
Efficiently synthesize the preparation method of StylissatinA native annulus heptapeptide the most according to claim 1, it is characterised in that the described first step specifically comprises the following steps that
1) take 500mg2 chlorine trityl resin, clean each 5 times with dimethylformamide, dichloromethane respectively, be subsequently adding dichloromethane and soak resin 15min；
2) mixed solution of the Fmoc-Phe-OH of 0.2mmol, dichloromethane and dimethylformamide is taken, it is sufficiently mixed dissolving, it is subsequently adding the N of 360ul, N-diisopropylethylamine, joins in the resin of step 2 after dissolving, react 1h in 35 DEG C of gas bath isothermal vibration shaking tables, take out reactant liquor, clean with dimethylformamide, dichloromethane respectively, close resin with methanol, Yong dimethylformamide, dichloromethane, dimethylformamide cleaning resin；
3) adding the newly configured piperidine solution of 8ml20% in resin, first static 5min, then takes out, the piperidines adding 8ml20% is placed in constant temperature oscillator, concussion reaction 10min, takes out solution, cleans resin with dichloromethane, dimethylformamide respectively；
4) 1mmolFmoc-Pro-OH is taken, 365mg1-hydroxybenzotriazole, 8ml dimethylformamide, it is sufficiently mixed dissolving, add 365ulN, N-diisopropylethylamine, shake activation 1min, it is then added in the resin of step 3 concussion reaction 1h, take out reactant liquor, respectively with dimethylformamide, dichloromethane, dimethylformamide cleans resin, the newly configured piperidine solution of 8ml20% is added in resin, first static 5min, then take out, the piperidines adding 8ml20% is placed in constant temperature oscillator, concussion reaction 10min, solution is taken out, respectively with dichloromethane, dimethylformamide cleans resin；
5) method repeating step 4, is coupled to Fmoc-Ile-OH, Fmoc-Pro-OH, Fmoc-Phe-OH, Fmoc-Tyr (tBu)-OH, Fmoc-Ile-OH on resin successively；
6) by acetic acid: trifluoroethanol: the mixed solution 15ml of dichloromethane=1:2:16 adds in the resin of step 5,35 DEG C of constant temperature oscillation reaction 2-4h, obtain linear peptides.
Efficiently synthesize the preparation method of StylissatinA native annulus heptapeptide the most according to claim 1, it is characterised in that described second step specifically comprises the following steps that
1) 0.5mmol hexafluorophosphoric acid benzotriazole-1-base-epoxide tripyrrole alkyl phosphorus, 0.5mmol1-hydroxy benzo triazole, 1mmolN are taken, N-diisopropylethylamine, join in dichloromethane, N-Methyl pyrrolidone, ultrasonic dissolution, add 150ml anhydrous methylene chloride, prepare mixed solution, be placed in 0 DEG C；Taking 0.1mmol linear peptides anhydrous methylene chloride to dissolve, making linear peptides concentration is 0.5mg/ml, is slowly dropped in mixed solution by linear peptides solution, stirring reaction, obtains white solid powder shape product；
2) white solid powder shape product is joined (trifluoroacetic acid/phenol/water/diisopropylsilyl=88/5/5/2) mixed solution of 10ml, reacts 3 hours in 35 DEG C of isothermal vibrations, obtain target compound.
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| CN109160940A (en) * | 2018-08-15 | 2019-01-08 | 成都医学院 | The preparation method of one seeds algae blueness collarium octapeptide Samoamide A |
| CN117486993A (en) * | 2023-04-23 | 2024-02-02 | 山东第一医科大学(山东省医学科学院) | Staple peptide and preparation method and application thereof |
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| CN117486993A (en) * | 2023-04-23 | 2024-02-02 | 山东第一医科大学(山东省医学科学院) | Staple peptide and preparation method and application thereof |
| CN117486993B (en) * | 2023-04-23 | 2024-03-26 | 山东第一医科大学(山东省医学科学院) | Staple peptide and preparation method and application thereof |
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