CN105815248B - Terminal equipment for distinguishing male and female sepiella maindroni - Google Patents
Terminal equipment for distinguishing male and female sepiella maindroni Download PDFInfo
- Publication number
- CN105815248B CN105815248B CN201610186617.6A CN201610186617A CN105815248B CN 105815248 B CN105815248 B CN 105815248B CN 201610186617 A CN201610186617 A CN 201610186617A CN 105815248 B CN105815248 B CN 105815248B
- Authority
- CN
- China
- Prior art keywords
- wrist
- length
- female
- sepiella maindroni
- male
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a terminal device for distinguishing male and female sepiella maindroni, which comprises a terminal body with a camera and a screen, wherein: the terminal is provided with a program which can shoot a photo of Sepiella maindroni with the sex to be identified and calculate the position of the wrist foot or the wrist-contacting end part from the photo; the left 3 wrist end, the left 4 wrist end, the left wrist touching end and the tail end are manually selected, the distance difference between the ends and the measuring base line is measured, the program calculates the left 3 wrist length, the left 4 wrist length, the left wrist touching length and the trunk length, and after the discriminant calculation is adopted, the categories are discriminated according to the maximum values obtained by F1 and F2, wherein F1 is male and F2 is female. The judgment by adopting the judgment formula has the advantages of high judgment accuracy, intuitive and quick judgment, easy operation and no damage to organisms to be identified. Therefore, the invention has the advantages of accurate result, simple operation and no harm to organisms.
Description
Technical Field
The invention relates to a marine organism gender identification device, in particular to a terminal device for identifying male and female sepiella maindroni.
Background
Sepiella maindroni (Sepiella maindroni)Sepiella japonica) Belongs to the phyla of mollusca, cephalopoda, sepiidae and sepiella, is one of the important marine fishery economic types in China, is one of the four marine products in east China, and has the highest annual yield of more than 6 million tons in Zhejiang in history (Dongzhi, 1988). But since the end of the 70 s of the 20 th century, the resource amount of the method seriously declines due to over-fishing and damage of water area conditions; in the middle and late 80 s, Sepiella maindroni in the coastal fishing ground of Zhejiang could not have developed a fishing flood (Wu Heng et al, 2010). Scholars at home and abroad mainly have resource status (Tang Yi Min et al, 1986), biology (Jiangxin et al, 2007; Chang kang Mei et al, 2008; Jiangxin et al, 2008; Zhang Jian et al, 2011), and breeding habits (Wada et al, Shi Xianmin et al, Shi Xianxi, Shi Xian Shiet al2006; wu Fang et al, 2010) and propagation and cultivation techniques (Wuet al2010, panhuan collar, etc. 2015). Since 2006, large-scale proliferation and releasing of fertilized eggs and larvae of sepiella maindroni was performed along the coast of zhejiang (prunella et al, 2011). Statistical data show that the proliferation and releasing work is effective, and a certain proliferation effect is achieved after large-scale releasing. With the development of the value-added release task, higher requirements are provided for the cultivation work of the high-quality sepiella maindroni parents. But up to now related to manThe research on the breeding way of the high-quality parent of the sepiella maindroni has not been reported yet. The morphological analysis of Sepiella maindroni is an important content of culture and breeding research and is also the basis for carrying out gender difference analysis and gender identification. By identifying the sex of the male and female parents, the method not only can reduce the feeding cost for parent cultivation, but also can reduce the number and cost for harvesting the parents, and is one of the important prerequisites for artificial seedling (Okamura)et al,2008). In addition, the difference of growth rate is obvious in the fish culture neutral status and the two status, the monogamy culture (Li Ming Zi, 2014) can be realized by sex control, and the monogamy culture is an ideal method for solving a series of problems of hindering large-scale production, such as precocious puberty, fast propagation, high density, slow growth, uneven specification and the like. In the process of Sepiella maindroni culture production, cannibalism phenomenon exists, particularly in the sexual maturity mating stage, the male fights for the mating right (Wu Heiwen et al, 2010), and the male mortality rate in the breeding stage is high (Correia)et al,2005). Therefore, the development of the artificial large-scale aquaculture industry of the Sepiella maindroni is restricted.
Current research on cephalopod morphology is mainly focused on different population comparisons: the research on the soft fishes in different geographic positions of the Pacific ocean is compared by a morphological method, such as Chenxin army and the like (2002), Li thinking and the like (2010), Chenfeng and the like (2011); zhengxiadong et al (2002) on Sepiella maindroni (Sepiella maindroni) coastal in south China (Sepiella maindroni) Phenotypic variation analysis is carried out; kassahn et al (2003) used molecular labeling and morphological methods to treat Australia giant cuttlefish (A. Mey.) (2003)Sepia apama) The population structure and species status are studied; tehranigar et al (2011) morphologically treat sepia pharaonisSepia Pharaonis) And carrying out group division.
Disclosure of Invention
The invention aims to provide terminal equipment for distinguishing male and female sepiella maindroni, which has accurate result, simple operation and no harm to organisms, aiming at the prior art.
The technical scheme adopted by the invention for solving the technical problems is as follows: differentiate terminal equipment of male and female of sepiella maindroni, including the terminal fuselage that has camera and screen, wherein: the terminal is provided with a program which can shoot a photo of Sepiella maindroni with the sex to be identified and calculate the position of the wrist foot or the wrist-contacting end part from the photo; through the artificial 3 wrist tip of selecting, left 4 wrist tip, left wrist of touching tip, tail end, through measuring the distance difference between above-mentioned tip and the measurement base line, the program calculates left 3 wrist length, left 4 wrist length, left wrist length, trunk length to adopt the following expression to calculate:
male: f1= 223.42X 3+ 165.85X 4+ 7.951X 5-108.06；
Female: f2= 151.48X 3+ 92.71X 4+ 19.19X 5-60.38；
In the above discriminantX 3The left wrist length/trunk length is used for replacing,X 4The left 4 wrist length/trunk length is used for replacing,X 5Substituting left antennal length/trunk length as discrimination parameter into the above discriminant, and discriminating the category according to the maximum values obtained from F1 and F2, wherein F1 is male and F2 is female. The judgment by adopting the judgment formula has the advantages of high judgment accuracy, intuitive and quick judgment, easy operation and no damage to organisms to be identified.
In order to optimize the technical scheme, the adopted measures further comprise: the position of the measurement baseline is artificially selected or calculated by averaging the positions of the base parts of the wrist and the foot. The measurement baseline is manually moved or clicked on the screen. Or the terminal equipment can be used for searching the tail end and the measurement baseline of each wrist and foot of the cuttlefish in a software calculation mode after photographing. Then manually selecting or selecting the tail end of the wrist foot, tail end and wrist-touching tail end of No. 3 or 4. Then, the program calculates the length by itself and substitutes the calculated length into the F1 and F2 discriminants, thereby performing gender determination. Treating Sepiella maindroni to be sex-identified with ice bath for 1-5 min before measurement; the ice bath liquid contains 160mg/L of MS-222 according to parts by weight. Since the cuttlefish is violent and spits ink, anesthesia and sedation should be done before the measurement. Before the sex-identifying Sepiella maindroni is measured, a mixture containing 1-5 parts by weight of vitamin C, 0.01-1 part by weight of MS-222, 0.01-0.5 part by weight of methionine and 10-50 parts by weight of solvent is soaked by absorbent cotton and is applied to the position near the connecting part of the mantle and the head and the feet of the Sepiella maindroni, and the treatment time is 1-5 minutes. The solvent is selected from clean seawater or yogurt. The mode that adopts the cotton wool comparatively saves the medicine to can reuse this cotton wool to carry out a lot of and handle, the portability is better.
The terminal is provided with a program which can shoot the photo of the Sepiella maindroni with the sex to be identified and calculate the position of the wrist foot or the wrist touching end part from the photo; the left 3 wrist end part, the left 4 wrist end part, the left wrist touching end part and the tail end part are manually selected, the distance difference between the end parts and the measuring base lines is measured, the program calculates the left 3 wrist length, the left 4 wrist length, the left wrist touching length and the trunk length, and the expression is adopted for calculation. In the above discriminants, the categories were discriminated from the maximum values obtained by F1 and F2 by substituting X3 for left 3 wrist length/trunk length, X4 for left 4 wrist length/trunk length, and X5 for left wrist length/trunk length as discriminant parameters, wherein F1 is male and F2 is female. The judgment by adopting the judgment formula has the advantages of high judgment accuracy, intuitive and quick judgment, easy operation and no damage to organisms to be identified. Therefore, the invention has the advantages of accurate result, simple operation and no harm to organisms.
Drawings
FIG. 1 is a schematic diagram of a terminal in use;
FIG. 2 is a diagram showing the morphological measurement index of Sepiella maindroni;
FIG. 3 is a schematic diagram showing the mating pattern of Sepiella maindroni;
FIG. 4 is a frequency chart showing the discrimination scores of male and female sepiella maindroni.
Detailed Description
The present invention will be described in further detail with reference to the following examples.
Example (b): referring to fig. 1 to 4, a terminal device for discriminating male and female sepiella maindroni includes a terminal body having a camera and a screen, wherein: the terminal is provided with a program which can shoot a photo of Sepiella maindroni with the sex to be identified and calculate the position of the wrist foot or the wrist-contacting end part from the photo; through the artificial 3 wrist tip of selecting, left 4 wrist tip, left wrist of touching tip, tail end, through measuring the distance difference between above-mentioned tip and the measurement base line, the program calculates left 3 wrist length, left 4 wrist length, left wrist length, trunk length to adopt the following expression to calculate:
male: f1= 223.42X 3+ 165.85X 4+ 7.951X 5-108.06；
Female: f2= 151.48X 3+ 92.71X 4+ 19.19X 5-60.38；
In the above discriminantX 3The left wrist length/trunk length is used for replacing,X 4The left 4 wrist length/trunk length is used for replacing,X 5Substituting left antennal length/trunk length as discrimination parameter into the above discriminant, and discriminating the category according to the maximum values obtained from F1 and F2, wherein F1 is male and F2 is female. The judgment by adopting the judgment formula has the advantages of high judgment accuracy, intuitive and quick judgment, easy operation and no damage to organisms to be identified. The position of the measurement baseline is artificially selected or calculated by averaging the positions of the base parts of the wrist and the foot. The measurement baseline is manually moved or clicked on the screen. Or the terminal equipment can be used for searching the tail end and the measurement baseline of each wrist and foot of the cuttlefish in a software calculation mode after photographing. Then manually selecting or selecting the tail end of the wrist foot, tail end and wrist-touching tail end of No. 3 or 4. Then, the program calculates the length by itself and substitutes the calculated length into the F1 and F2 discriminants, thereby performing gender determination. Treating Sepiella maindroni to be sex-identified with ice bath for 1-5 min before measurement; the ice bath liquid contains 160mg/L of MS-222 according to parts by weight. Since the cuttlefish is violent and spits ink, anesthesia and sedation should be done before the measurement. Before the sex-identifying Sepiella maindroni is measured, a mixture containing 1-5 parts by weight of vitamin C, 0.01-1 part by weight of MS-222, 0.01-0.5 part by weight of methionine and 10-50 parts by weight of solvent is soaked by absorbent cotton and is applied to the position near the connecting part of the mantle and the head and the feet of the Sepiella maindroni, and the treatment time is 1-5 minutes. The solvent is selected from clean seawater or yogurt. The absorbent cotton is more drug-saving, can be reused for multiple treatments, and is more portableGood results are obtained.
The observation of video recording shows that the male Sepiella maindroni has obvious coupling behavior and can be divided into three stages: (1) the self-exhibition and chasing behavior is that the body color of the mature male back is more bright than that of the female, and the white stripes on the back and the white continuous lines on the base of the fins are more obvious. At the same time, the first pair of wrists was raised gradually closer to the female to attract attention. In the process of idol, males are in the position of active selection and pursuit, while female cuttlefish has the behavior of accepting and escaping pairing. Once a female is selected by a mature male individual, a pursuit process of poor pursuit can be developed for the female, if the female cuttlefish receives the sexual behavior of the male, a swimming accompanying behavior can occur, namely the male swims along with the female, and when other male cuttlefishes approach, the male can spread 4 pairs of long wrists to drive other male cuttlefishes to approach. This concomitant behavior can effectively prevent other males from robbing the female. (2) In the fighting action, the same female is contended for by the male, and the fighting between the two sexes occurs. The extended 4 pairs of wrists of the male can be used for attracting the female and has the function of deterring other males. (3) Mating behavior, after a period of time following the involution of the female, shows a very mild state if the female does not respond excessively. Indicating that the female has received the idol activity. The male and female can mate, and the sepiella maindroni adopts a head-to-head mating mode (as shown in figure 3): the male and female cuttlefishes have opposite heads, the male fixes the female heads by using the first pair and the second pair of wrists, then the spermatophore is conveyed to the seminal vesicle of the female cuttlefishes by using the stemmed wrists, and the mating time is 5-15S generally. The mated males remain associated around the female, impeding mating with other males. Through research, the ratio of 4 pairs of wrist length to carcass length of a male is considered to be larger than that of a female, which is related to the mode that 4 pairs of wrists hold each other when sepiella maindroni is mated. The male has the initiative of preferential selection when the sepiella maindroni selects the spouse in the breeding period, and is adaptive to the fighting behavior when the male contends for the spouse. Females distribute energy primarily in the reproductive offspring during sexual maturity, while males are used primarily for growth.
TABLE 1 statistics of amphoteric measured trait parameters of Sepiella maindroni
If F1> F2, the male individual is obtained, and otherwise, the female individual is obtained. As shown in fig. 3, only 8 cuttlefishes have wrong sex determination, and the determination rate is 5%, i.e., the determination success rate reaches 95.00%.
The male sex is much lower than the female sex in breeding investment, and larger female individuals have higher fertility and breeding success rate (Yang Cheng et al, 2015; Angilletta)et al,2000). In this study, both the gonadal and hepatic indices of females were significantly greater than those of males (P)<0.05) while the total body weight and meat yield are significantly less than those of males (P)<0.05) for statistics see table 1. It is clear that male sepiella maindroni adopts a strategy of gaining the mating right by increasing the weight under the pressure of sexual selection, but the energy distribution strategy is greatly different from that of female. Namely: males have significantly greater energy input in muscle building than females, and thus can increase the ability to acquire food and mating power by enhancing physical performance, thereby achieving compliance with selection pressure and increasing reproductive fitness. While female energy allocation strategies use energy for the growth of reproductive-related organs, female gonadal indices are significantly greater than male (P)<0.05). Meanwhile, there may be a close response and transmission relationship between the growth and development of oocytes in ovaries and liver energy supply (Pinghuang collar, 2015), which indicates that the energy stored in the liver of Sepiella maindroni is related to the reproductive function of females. Females must invest more energy in reproductive organs than males to comply with reproductive selection pressure and to increase reproductive fitness, thereby also limiting their growth.
Cephalopods are male and female allogenic molluscs that have reproductive behaviors such as antagonism, loving, and mating during the reproductive process of male transferring spermatophore to female (chenxin et al, 2009). Sepiella maindroni has higher and other complex sexual behaviors (Wu Heiwen et al, 2010), the mating mode adopts a head-to-head mode, the mating process is similar to that of Sepiella tigerini, the 1 st and the 2 nd wrists are adopted to wrap the female head, and then the styled wrists of the left 4 are utilized to send the spermatophore into the female seminal vesicle (Chengdao et al, 2013). In this study, it is just described the evolutionary position of the male carpal length in mating behaviour: the left 1, 2, 3 and 4 wrist lengths of males are all significantly greater than females (P < 0.05). Meanwhile, the 4 standardized wrist length indicators above were also significantly larger than females (P <0.05) excluding individual size differences. Moreover, the variables of the discrimination equation of male and female sepiella maindroni are reflected, and the sepiella maindroni has obvious amphiprotic abnormity on the size of the wrist length in the mature stage.
The software adopts the following algorithm steps of carrying out binarization processing on the photo (preferably putting the cuttlefish on a white board), and then carrying out boundary search on the wrist foot and the wrist touch to find out the convex end part. And respectively marking red circles on the found end parts, so that an operator can select the left 3 wrist end part, the left 4 wrist end part, the left wrist touching end part and the tail end part in sequence. On the other hand, a measurement baseline for the measurement needs to be determined. The baseline can be manually set by manually and directly clicking, and can be directly slid on the screen by using a finger, and the measurement baseline moves along with the fingertip. After the selection is finished, the program directly calculates the distance difference between the left 3 wrist end, the left 4 wrist end, the left wrist-touching end, the tail end and the measurement base line. And finally substituting the formula for gender judgment. The measurement baseline can also be obtained automatically, and can be estimated by searching the image for the base of the carpometacarpal fork and then averaging the positions, but the relative accuracy is lower than that set manually.
While the invention has been described in connection with a preferred embodiment, it is not intended to limit the invention, and it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the spirit and scope of the invention.
Claims (2)
1. The terminal equipment for distinguishing male and female sepiella maindroni comprises a terminal machine body with a camera and a screen, and is characterized in that: the terminal is provided with a program which can shoot a photo of Sepiella maindroni with the sex to be identified and calculate the position of the wrist foot or the wrist-contacting end part from the photo; through the artificial 3 wrist tip of selecting, left 4 wrist tip, left wrist of touching tip, tail end, through measuring the distance between above-mentioned tip and the measurement base line, the program calculates left 3 wrist length, left 4 wrist length, left wrist length, trunk length to adopt the following expression to calculate:
F1＝223.42X3+165.85X4+7.951X5-108.06；
F2＝151.48X3+92.71X4+19.19X5-60.38；
in the above discriminant, X is3Replacement of left 3 wrist length/trunk length, X4Replacement of left 4 wrist length/trunk length, X5Substituting left wrist length/trunk length as discrimination parameter into the above discriminant, and discriminating the category according to the maximum values obtained from F1 and F2, such as F1>F2, is male, otherwise is female;
treating Sepiella maindroni to be sex-identified with ice bath for 1-5 min before measurement, wherein the ice bath solution contains 160mg/L MS-222; and then soaking a mixture containing 1-5 parts by weight of vitamin C, 0.01-1 part by weight of MS-222, 0.01-0.5 part by weight of methionine and 10-50 parts by weight of solvent by using absorbent cotton, and applying the mixture to the vicinity of the connection part of the mantle and the head and feet of the Sepiella maindroni for 1-5 minutes, wherein the solvent is selected from clean seawater or yoghourt.
2. The terminal device for discriminating male and female sepiella maindroni according to claim 1, wherein: the position of the measurement baseline is artificially selected or calculated by averaging the positions of the split parts of the wrist and foot bases.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610186617.6A CN105815248B (en) | 2016-05-26 | 2016-05-26 | Terminal equipment for distinguishing male and female sepiella maindroni |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610186617.6A CN105815248B (en) | 2016-05-26 | 2016-05-26 | Terminal equipment for distinguishing male and female sepiella maindroni |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105815248A CN105815248A (en) | 2016-08-03 |
| CN105815248B true CN105815248B (en) | 2020-09-08 |
Family
ID=56524969
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610186617.6A Active CN105815248B (en) | 2016-05-26 | 2016-05-26 | Terminal equipment for distinguishing male and female sepiella maindroni |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105815248B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107897051B (en) * | 2017-12-14 | 2020-03-24 | 浙江海洋大学 | Unisexual culture method for improving cuttlefish culture rate |
-
2016
- 2016-05-26 CN CN201610186617.6A patent/CN105815248B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN105815248A (en) | 2016-08-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Felip et al. | Comparative growth performance of diploid and triploid European sea bass over the first four spawning seasons | |
| Kiriyakit et al. | Successful hybridization of groupers (Epinephelus coioides x Epinephelus lanceolatus) using cryopreserved sperm | |
| Curtis et al. | Observations on monosex culture of redclaw crayfish Cherax quadricarinatus von Martens (Decapoda: Parastacidae) in earthen ponds | |
| Asakura | Sexual differences in life history and resource utilization by the hermit crab | |
| Peixoto et al. | Effects of age and size on reproductive performance of captive Farfantepenaeus paulensis broodstock | |
| Mi et al. | Testis asymmetry and sperm length in Rhacophorus omeimontis | |
| McCambridge et al. | Effects of autotomy compared to manual declawing on contests between males for females in the edible crab cancer pagurus: Implications for fishery practice and animal welfare | |
| CN102187833B (en) | Method for preventing and controlling shrimp communicable diseases by Epinephelus coioids | |
| Bart et al. | Spermatophore cryopreservation and artificial insemination of black tiger shrimp, Penaeus monodon (Fabricius) | |
| CN102077798A (en) | Biological prevention and control method for controlling prawn diseases through grass carps | |
| CN103478023A (en) | Artificial propagation technology for Rhodeus sinensis | |
| Park et al. | Growth performance, morphometric traits and gonad development of induced reciprocal diploid and triploid hybrids between the mud loach (Misgurnus mizolepis Günther) and cyprinid loach (Misgurnus anguillicaudatus Cantor) | |
| CN104012437A (en) | Rhodeus sinensis artificial propagation method | |
| Habashy | On the breeding behaviour and reproduction of the freshwater prawn, M acrobrachium rosenbergii (de Man 1879)(D ecapoda‐C rustacea) under laboratory conditions | |
| CN105815248B (en) | Terminal equipment for distinguishing male and female sepiella maindroni | |
| CN105815249B (en) | Method for distinguishing male and female sepiella maindroni | |
| Sato | Plausible causes for sperm-store variations in the coconut crab Birgus latro under large male-selective harvesting | |
| CN102077795A (en) | Biological prevention and control method for controlling shrimp diseases through clarias leather | |
| Jiang et al. | Phenotypic evaluation of two genetically improved strains selected from the reciprocal hybrids of Crassostrea gigas and C. angulata | |
| Strüssmann et al. | Triploidy induction in an atherinid fish, the pejerrey (Odontesthes bonariensis) | |
| Hunter et al. | Recent studies on the crawfish Palinurus elephas in South Wales and Cornwall | |
| Kim et al. | Occurrence of a natural intergeneric hybrid between Rhodeus pseudosericeus and Acheilognathus signifer (Pisces: Cyprinidae) from the Namhangang (river), Korea | |
| Chopin et al. | Seaweed aquaculture–from the global, mostly Asian, picture to the opportunities and constraints of the Canadian scene | |
| Tave | Effective breeding efficiency: An index to quantify the effects that different breeding programs and sex ratios have on inbreeding and genetic drift | |
| CN105519460A (en) | A biological control method of controlling prawn diseases through grass carps |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |