CN105806894A - Electrical impedance method for measuring soluble sugar content in catalpa bungei stem leaf and cold resistance of catalpa bungei - Google Patents
Electrical impedance method for measuring soluble sugar content in catalpa bungei stem leaf and cold resistance of catalpa bungei Download PDFInfo
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Abstract
The present invention provides an electrical impedance method for measuring soluble sugar content in catalpa bungei stem leaf. The method comprises the steps of: detecting an electrical impedance spectroscopy of a catalpa bungei stem leaf sample to be tested; according to the electrical impedance spectroscopy of the sample to be tested, determining an appropriate equivalent circuit; according to the equivalent circuit, fitting the data of the electrical impedance spectroscopy of the sample to be tested to obtain electrical impedance parameters of the sample to be tested; and according to a regression model of the predetermined electrical impedance parameters of the catalpa bungei stem leaf with soluble sugar content, and the electrical impedance parameters of the sample to be tested, acquiring the soluble sugar content of the catalpa bungei stem leaf. The method provided by the invention establishes a regression model of the electrical impedance parameters and soluble sugar content of the catalpa bungei stem leaf, thus calculating the soluble sugar content of the catalpa bungei stem leaf based on the measured impedance parameters. The method can realize fast real-time diagnosis of the soluble sugar content and can obtain the cold resistance of the catalpa bungei stem leaf through the Logistic equation.
Description
Technical field
The present invention relates to plant component content detection technical field, particularly relate to a kind of electrical impedance method and measure Chinese catalpa stem and leaf soluble sugar content and the cold resistant method of Chinese catalpa.
Background technology
Plant is closely related with physiological and biochemical activities such as own cells film, enzymatic protective system, osmotic adjustments to the adaptation of low temperature stress.Letter makes into etc. to be thought, plant relies primarily on soluble sugar at the low temperature stress initial stage and carries out Osmoregulation, and when coerce deepen further time, other infiltration materials such as soluble protein just work, and therefore soluble sugar content is frequently as plant identification winter resistance physical and chemical index.The change of soluble sugar content in detection promptly and accurately and diagnosis of plant body, contributes to understanding plant physiology changed condition, has great importance for monitoring plant cold resistance.
Chinese catalpa (CatalpaSpp.) being Chinese distinctive germ plasm resource, be not only important commerical tree species, and be important urban greening species, application approach is extensive.Chinese catalpa have the title of " wood king ", be distributed in from the east of beach, west to Gansu, beginning Yunnan, south, north is in the broad area on Great Wall, happiness light, more cold-resistant, grow average temperature of the whole year 10 DEG C ~ 15 DEG C, not only can build fast-growing, high-yield woods, to obtain high quality timber, it is also possible to as shelter-forest water conservation soil conservation, as urban greening species, antipollution power is strong, and ornamental value is high.Along with people afforest the lifting of consciousness, Chinese catalpa will be widely used in northern greening as cold-resistant excellent ornamental tree species.
In order to study the Chinese catalpa adaptability to north temperature, need its cold performance is measured.In view of prior art has been found that the relation between soluble sugar content and winter resistance, the detection for soluble sugar content is capable of the cold resistant mensuration of Chinese catalpa.And the most traditional method measuring plant soluble sugar mainly has anthrone method and phynol method, but traditional method is the most loaded down with trivial details, laborious, time-consuming, and has destructiveness and hysteresis quality, it is impossible to requirement quick, real-time in enough satisfied production.
Summary of the invention
It is an object of the invention to provide a kind of electrical impedance method and measure soluble sugar content and the cold resistant method of Chinese catalpa in Chinese catalpa stem and leaf, the method that the present invention provides is capable of quick, the real time measure of soluble sugar content in Chinese catalpa stem and leaf, and achieves Accurate Determining cold resistant to Chinese catalpa.
The invention provides a kind of electrical impedance method and measure the method for soluble sugar content in Chinese catalpa stem and leaf, comprise the following steps:
The Electrical Impedance Spectroscopy of detection Chinese catalpa stem and leaf testing sample；
Electrical Impedance Spectroscopy according to described testing sample determines applicable equivalent circuit；
According to the described equivalent circuit determined, the Electrical Impedance Spectroscopy of described testing sample is carried out data matching, obtains the electrical impedance parameter of described testing sample；
According to predetermined Chinese catalpa stem and leaf electrical impedance parameter and the regression model of soluble sugar content and the electrical impedance parameter of described testing sample, obtain soluble sugar content in Chinese catalpa stem and leaf.
Preferably, described electrical impedance parameter includes alternating-current resistance rate, low-frequency resistance rate, Extracellular resistance, intracellular resistance, relaxation time and distribution of relaxation times coefficient.
Preferably, described equivalent circuit is single-DCE model.
Preferably, the regression model of the alternating-current resistance rate that regression model is Chinese catalpa stem of described Chinese catalpa stem electrical impedance parameter and soluble sugar content and soluble sugar content.
Preferably, the alternating-current resistance rate of described Chinese catalpa stem is independent variable with the alternating-current resistance rate of Chinese catalpa stem in the regression model of soluble sugar content, and soluble sugar content is dependent variable.
Preferably, the alternating-current resistance rate of described Chinese catalpa stem and the regression model of soluble sugar content are quadratic equation or cubic equation.
Preferably, the regression model of the distribution of relaxation times coefficient that regression model is Chinese catalpa leaf of described Chinese catalpa leaf electrical impedance parameter and soluble sugar content and soluble sugar content.
Preferably, the distribution of relaxation times coefficient of described Chinese catalpa leaf is independent variable with the distribution of relaxation times coefficient of Chinese catalpa leaf in the regression model of soluble sugar content, and soluble sugar content is dependent variable.
Preferably, the distribution of relaxation times coefficient of described Chinese catalpa leaf and the regression model of soluble sugar content are quadratic equation or cubic equation.
The invention provides a kind of electrical impedance method and measure the cold resistant method of Chinese catalpa, comprise the following steps:
The Electrical Impedance Spectroscopy of detection Chinese catalpa stem and leaf testing sample；
Electrical Impedance Spectroscopy according to described testing sample determines suitable equivalent circuit；
According to the described equivalent circuit determined, the Electrical Impedance Spectroscopy of described testing sample is carried out data matching, obtains the electrical impedance parameter of described testing sample；
The soluble sugar content of predetermined Chinese catalpa stem and leaf and the electrical impedance parameter of described testing sample are carried out correlation analysis, obtains and soluble sugar content dependency extremely significantly electrical impedance parameter；
According to described electrical impedance parameter extremely significant with soluble sugar content dependency, obtained the winter resistance of Chinese catalpa stem and leaf by Logistic Equation for Calculating.
The invention provides a kind of electrical impedance method and measure the method for soluble sugar content in Chinese catalpa stem and leaf, comprise the following steps: the Electrical Impedance Spectroscopy of detection Chinese catalpa stem and leaf testing sample；Electrical Impedance Spectroscopy according to described testing sample determines applicable equivalent circuit；According to the described equivalent circuit determined, the Electrical Impedance Spectroscopy of described testing sample is carried out data matching, obtains the electrical impedance parameter of described testing sample；According to predetermined Chinese catalpa stem and leaf electrical impedance parameter and the regression model of soluble sugar content and the electrical impedance parameter of described testing sample, obtain soluble sugar content in Chinese catalpa stem and leaf.The method that the present invention provides establishes the regression model between Chinese catalpa stem and the electrical impedance parameter of leaf and its soluble sugar content, thus be calculated the soluble sugar content in Chinese catalpa stem and leaf according to the electrical impedance parameter recorded, and the content being capable of soluble sugar is quick, real-time diagnosis.And, the present invention has also set up the relation between electrical impedance parameter and winter resistance change such that it is able to learn the winter resistance of Chinese catalpa stem and leaf according to the change of electrical impedance parameter, and the estimation for winter resistance change provides important theoretical foundation and technical support.
Accompanying drawing explanation
Fig. 1 is the Electrical Impedance Spectroscopy change curve of the spun gold Chinese catalpa stem that the embodiment of the present invention 1 obtains；
Fig. 2 is the Electrical Impedance Spectroscopy change curve of the spun gold Folium catalpae bungei that the embodiment of the present invention 2 obtains；
Fig. 3 is the change curve of soluble sugar content in spun gold Chinese catalpa stem during the Cold Acclimation that the embodiment of the present invention 7 obtains；
Fig. 4 is the change curve of soluble sugar content in spun gold Folium catalpae bungei during the Cold Acclimation that the embodiment of the present invention 8 obtains.
Detailed description of the invention
The invention provides a kind of electrical impedance method and measure the method for soluble sugar content in Chinese catalpa stem and leaf, comprise the following steps:
The Electrical Impedance Spectroscopy of detection Chinese catalpa stem and leaf testing sample；
Electrical Impedance Spectroscopy according to described testing sample determines applicable equivalent circuit；
According to the described equivalent circuit determined, the Electrical Impedance Spectroscopy of described testing sample is carried out data matching, obtains the electrical impedance parameter of described testing sample；
According to predetermined Chinese catalpa stem and leaf electrical impedance parameter and the regression model of soluble sugar content and the electrical impedance parameter of described testing sample, obtain soluble sugar content in Chinese catalpa stem and leaf.
The method that the present invention provides establishes the regression model between Chinese catalpa stem and the electrical impedance parameter of leaf and its soluble sugar content, thus be calculated the soluble sugar content in Chinese catalpa stem and leaf according to the electrical impedance parameter recorded, and it is capable of the content quick diagnosis of soluble sugar.
The present invention detects the Electrical Impedance Spectroscopy of Chinese catalpa stem and leaf testing sample.The present invention does not has special restriction to the selection of described Chinese catalpa stem and leaf testing sample, and those skilled in the art can be according to its representational testing sample of method choice known.In an embodiment of the present invention, the length of the testing sample of described Chinese catalpa stem is preferably 10
Mm ~ 30 mm, more preferably 15 mm ~ 20 mm；The testing sample of described Chinese catalpa leaf is preferably the Chinese catalpa leaf without vein；The testing sample of described Chinese catalpa leaf preferably includes, but is not limited to rectangle or circle；When the testing sample of described Chinese catalpa leaf is rectangle, the testing sample of described Chinese catalpa leaf is preferably dimensioned to be 4 mm × 10 mm.
The present invention does not has special restriction to the instrument that described electrical impedance measures, and uses electrical impedance instrument well known to those skilled in the art.In an embodiment of the present invention, described electrical impedance instrument can be model be the impedance instrument of HP4284A.In the present invention, described electrical impedance measures the electrode used and is preferably Ag/AgCl electrode；The most electrode coated gel of the present invention.In the present invention, the coating thickness of described electrode gel is preferably 0.5
Cm ~ 2 cm, more preferably 1 cm ~ 1.5 cm.The present invention does not has special restriction to described electrode and the source of electrode gel and kind, use electrical impedance measuring electrode well known to those skilled in the art and the commercial goods of electrode gel, as the U.S. can be used to produce RCI model electrode and the GEL100 model gel of U.S.'s product WPI brand of WPI brand.
Electrode, when being measured the testing sample of described Chinese catalpa stem and leaf, is preferably contacted by the present invention with the cross section of testing sample；When the electrode coated gel of described electrode surface, preferably the cross section of testing sample is contacted with electrode gel.
After obtaining the Electrical Impedance Spectroscopy of testing sample, the present invention determines applicable equivalent circuit according to the Electrical Impedance Spectroscopy of described testing sample.In the art, the determination of plant equivalent circuit is generally divided into two types: lumped model and distributed model.In the present invention, the Chinese catalpa stem obtained and the Electrical Impedance Spectroscopy of leaf testing sample are single arc, and the equivalent circuit determined is single-DCE model, and this belongs to the one of distributed model.
After determining suitable equivalent circuit, the Electrical Impedance Spectroscopy of described testing sample, according to the described equivalent circuit determined, is carried out data matching, obtains the electrical impedance parameter of described testing sample by the present invention.In an embodiment of the present invention, Cole-Cole model and the equivalent circuit determined is used to carry out data matching, concrete grammar can refer to " correlation analysis of Betula platyphylla Suk. root system soluble sugar and starch content and electrical impedance under water logging stress " (Meng Yu of the reports such as Meng Yu in prior art, Di Bao, Zhang Gang, Feng Xinguo, Xu Chengli, Tian Jun. Betula platyphylla Suk. root system soluble sugar and starch content and the correlation analysis of electrical impedance under water logging stress. Acta Biophysica Sinica, 2013,29 (6): 450 ~ 460.), particularly as follows:
The computing formula of electrical impedance sees Formulas I:
In Formulas I, Z is impedance；
RFor alternating-current resistance, in embodiments of the present invention, use 100
Chinese catalpa stem and leaf sample is encouraged by the alternating voltage of mv, the frequency of 80 Hz ~ 1 MHz, and the resistance obtained at frequency >=1000 Hz is alternating-current resistance；
R 1For low-frequency resistance, in embodiments of the present invention, 100mv alternating voltage, the frequency of 80 Hz ~ 1 MHz is used Chinese catalpa stem and leaf testing sample to be encouraged, in frequency ＜ 1000
The resistance obtained during Hz is low-frequency resistance；
iThe evolution of=-1, is complex operator；
ωFor angular frequency,ω=2×π×f, whereinfFor frequency；
τFor the relaxation time；
ψFor distribution of relaxation times coefficient.
The present invention uses Formula II to calculate extracellular resistanceR e:
R e =R+R 1Formula II；
In Formula II, R is alternating-current resistance, it is thus achieved that mode is with in Formulas IR；
R 1For low-frequency resistance, it is thus achieved that mode is with in Formulas IR 1。
The present invention uses formula III to calculate intracellular resistance R i :
In formula III,RFor alternating-current resistance, it is thus achieved that mode is with in Formulas IR；
R1For low-frequency resistance, it is thus achieved that mode is with in Formulas IR 1。
The present invention according to the sectional area of each sample and length normalization method, calculates each resistance parameter according to formula IV:
In formula IV,r x For the resistivity of each resistance, asr e For Extracellular resistance,r i For intracellular resistance；
R x For the resistance value measured, unit is Ω；
lFor sample length, unit is m；
AFor sample sectional area, unit is m2。
In the present invention, relaxation time and distribution of relaxation times coefficient do not make normalized.
In the present invention, described electrical impedance parameter preferably includes alternating-current resistance rater(Ω m), low-frequency resistance rater 1(Ω m), Extracellular resistancer e(Ω m), intracellular resistancer i (Ω m), relaxation timeτWith distribution of relaxation times coefficientψ。
After obtaining electrical impedance parameter, the present invention, according to predetermined Chinese catalpa stem and leaf electrical impedance parameter and the regression model of soluble sugar content and the electrical impedance parameter of described testing sample, obtains soluble sugar content in Chinese catalpa stem and leaf.The present invention studies discovery, there is certain dependency, hence set up the regression model between soluble sugar content and electrical impedance parameter between electrical impedance parameter value and the soluble sugar content therein of Chinese catalpa stem and leaf.
In the present invention, described Chinese catalpa stem electrical impedance parameter is preferably the alternating-current resistance rate of Chinese catalpa stem and the regression model of soluble sugar content with the regression model of soluble sugar content；It is furthermore preferred that the alternating-current resistance rate of described Chinese catalpa stem is independent variable with the alternating-current resistance rate of Chinese catalpa stem in the regression model of soluble sugar content, soluble sugar content is dependent variable；In an embodiment of the present invention, the alternating-current resistance rate of described Chinese catalpa stem and the regression model of soluble sugar content are quadratic equation or cubic equation.
In the present invention, described Chinese catalpa leaf electrical impedance parameter is preferably the distribution of relaxation times coefficient of Chinese catalpa leaf and the regression model of soluble sugar content with the regression model of soluble sugar content；It is furthermore preferred that the distribution of relaxation times coefficient of described Chinese catalpa leaf is independent variable with the distribution of relaxation times coefficient of Chinese catalpa leaf in the regression model of soluble sugar content, soluble sugar content is dependent variable；In an embodiment of the present invention, the distribution of relaxation times coefficient of described Chinese catalpa leaf and the regression model of soluble sugar content are quadratic equation or cubic equation.
In the present invention, described Chinese catalpa stem and leaf electrical impedance parameter is set up the most in accordance with the following methods with the regression model of soluble sugar content:
Measure soluble sugar content in Chinese catalpa stem and leaf；
Measure Chinese catalpa stem and the Electrical Impedance Spectroscopy of leaf；
Applicable equivalent circuit is determined according to described Electrical Impedance Spectroscopy；
According to the described equivalent circuit determined, described Electrical Impedance Spectroscopy is carried out data matching, obtain the electrical impedance parameter of Chinese catalpa stem and leaf；
The soluble sugar content of described Chinese catalpa stem and leaf and described electrical impedance parameter are carried out correlation analysis, obtains the electrical impedance parameter of soluble sugar content pole significant correlation with described Chinese catalpa stem and leaf；
The electrical impedance parameter of the soluble sugar content of described Chinese catalpa stem and leaf Yu described pole significant correlation is carried out regression analysis, obtains the regression model of Chinese catalpa stem and leaf electrical impedance parameter and soluble sugar content.
The present invention detects soluble sugar content in Chinese catalpa stem and leaf.The present invention does not has special restriction to the detection method of described soluble sugar content, uses the detection technique scheme of soluble sugar content well known to those skilled in the art to detect Chinese catalpa stem and leaf.In an embodiment of the present invention, can use anthrone development process that soluble sugar content in Chinese catalpa stem and leaf is measured.The present invention is before being measured Chinese catalpa stem and leaf, preferably by Chinese catalpa stem and leaf cleaning post-drying；The present invention does not has special restriction to the method for described cleaning, the method using clean plant sample well known to those skilled in the art, as deionized water can be used to clean stem and the leaf of Chinese catalpa, dries after drying again.The present invention does not has special restriction to the method for described drying, uses the technical scheme of drying well known to those skilled in the art；In the present invention, the described equipment used of drying is preferably baking oven；The temperature of described drying is preferably 70 DEG C ~ 100 DEG C, more preferably 75 DEG C ~ 90 DEG C, most preferably 80 DEG C ~ 85 DEG C；The time of described drying is preferably 45 h ~ 3 d, more preferably 2 d ~ 2.5 d.
The present invention is preferably measured according to the assay method of the electrical impedance described in technique scheme, the assay method of electrical impedance parameter, obtains the electrical impedance parameter of Chinese catalpa stem and leaf.
After obtaining the electrical impedance parameter of Chinese catalpa stem and the soluble sugar content of leaf and Chinese catalpa stem and leaf, described soluble sugar content and electrical impedance parameter are carried out correlation analysis by the present invention, obtain the electrical impedance parameter of soluble sugar content pole significant correlation with described Chinese catalpa stem and leaf.In the present invention, described pole significant correlation is preferably pole significant correlation in 0.01 level.In the present invention, it is preferably the alternating-current resistance rate of Chinese catalpa stem with the electrical impedance parameter of soluble sugar content pole significant correlation in Chinese catalpa stemr；It is preferably the distribution of relaxation times coefficient of Chinese catalpa leaf with the electrical impedance parameter of soluble sugar content pole significant correlation in Chinese catalpa leafψ。
Obtain with the electrical impedance parameter of the soluble sugar content pole significant correlation of described Chinese catalpa stem and leaf after, the electrical impedance parameter of the soluble sugar content of described Chinese catalpa stem and leaf Yu described pole significant correlation is carried out regression analysis by the present invention, obtains the regression model of Chinese catalpa stem and leaf electrical impedance parameter and soluble sugar content.In the present invention, described regression analysis can be linear regression analysis, it is also possible to for nonlinear regression analysis；Described nonlinear regression analysis preferably includes logarithmic regressions analysis, Quadratic Regression Analysis, cube regression analysis, power regression analysis and exponential regression analysis.
In an embodiment of the present invention, the linear regression model (LRM) of the Chinese catalpa stem obtained is yStem =
0.4724r+ 9.9964；The logistic regression models of Chinese catalpa stem is yStem = 3.5626Ln(r) + 8.0709；The quadratic regression model of Chinese catalpa stem is yStem =
-0.0177r 2 + 0.889r+ 8.5912；Cube regression model of Chinese catalpa stem is yStem =
-0.0012r 3 + 0.0316r 2 + 0.351r +
9.6032；The power regression model of Chinese catalpa stem is yStem =
8.8429r 0.2527；The Exponential Regression Model of Chinese catalpa stem is yStem = 10.202e0.0329r ；In the regression model of Chinese catalpa stem, yStemFor soluble sugar content in Chinese catalpa stem,rAlternating-current resistance rate for Chinese catalpa stem.
In an embodiment of the present invention, the linear regression model (LRM) of the Chinese catalpa leaf obtained is yLeaf=
-0.399ψ+ 21.997；The logistic regression models of Chinese catalpa leaf is yLeaf = -4.4759Ln(ψ) +
27.766；The quadratic regression model of Chinese catalpa leaf is yLeaf= 0.0499ψ 2 -
1.6055ψ+ 27.742；Cube regression model of Chinese catalpa leaf is yLeaf = 0.0068ψ 3 -
0.2023ψ 2 + 1.2192ψ+ 18.486；The power regression model of Chinese catalpa leaf is yLeaf = 31.537ψ -0.2631；The Exponential Regression Model of Chinese catalpa leaf is yLeaf =
22.498e-0.0236 ψ ；In the regression model of Chinese catalpa leaf, yLeafFor soluble sugar content in Chinese catalpa leaf,ψDistribution of relaxation times coefficient for Chinese catalpa leaf.
After obtaining the electrical impedance parameter of Chinese catalpa stem and leaf and the regression model of soluble sugar content, the electrical impedance parameter of the testing sample that the present invention obtains according to technique scheme and described regression model, it is calculated soluble sugar content in Chinese catalpa stem and leaf.The Chinese catalpa stem set up respectively of method that the present invention provides and the regression model of Chinese catalpa leaf, thus obtain soluble sugar content in Chinese catalpa stem and Chinese catalpa leaf.
The method that the present invention provides establishes the regression model between electrical impedance parameter and soluble sugar content, owing to electrical impedance parameter is unrelated with sample size, only need to consider evaluated error, it is possible to the objective status of reflection plant, and measurement result accuracy rate is high.
In prior art often using soluble sugar content as plant identification winter resistance physical and chemical index, in detection promptly and accurately and diagnosis of plant body, the change of soluble sugar content, contributes to understanding the change of plant physiology situation.Therefore, the method detection that the present invention provides obtains soluble sugar content, it is possible to reflect Chinese catalpa stem and the winter resistance of Chinese catalpa leaf.Concrete, present invention also offers a kind of cold resistant detection method of Chinese catalpa, comprise the following steps:
Detection Chinese catalpa stem and the Electrical Impedance Spectroscopy of leaf testing sample；
Electrical Impedance Spectroscopy according to described testing sample determines suitable equivalent circuit；
According to the described equivalent circuit determined, the Electrical Impedance Spectroscopy of described testing sample is carried out data matching, obtains the electrical impedance parameter of described testing sample；
The soluble sugar content of predetermined Chinese catalpa stem and leaf and the electrical impedance parameter of described testing sample are carried out correlation analysis, obtains and soluble sugar content dependency extremely significantly electrical impedance parameter；
According to described electrical impedance parameter extremely significant with soluble sugar content dependency, obtained the winter resistance of Chinese catalpa stem and leaf by Logistic Equation for Calculating.
The testing sample of Chinese catalpa stem and leaf, in order to detect the winter resistance of Chinese catalpa stem and leaf, is preferably carried out freezing processing by the present invention, to be measured after defrosting.The present invention preferably says that Chinese catalpa stem and leaf are cleaned to dry and is placed in plastic bag, and wherein sprays into deionized water mutually, prevents material supercool.
In the present invention, described freezing processing is preferably provided with 5 ~ 9 thermogrades, more preferably arranges 7 thermogrades；In the thermograde of described Chinese catalpa stem freezing processing, the first gradient temperature is preferably 4 DEG C, as a control group；Second gradient temperature is preferably-3 DEG C ~-8 DEG C；3rd gradient temperature is preferably-6 DEG C ~-16 DEG C；4th gradient temperature is preferably-10 DEG C ~-24 DEG C；5th gradient temperature is preferably-15 DEG C ~-34 DEG C；6th gradient temperature is preferably-20 DEG C ~-46 DEG C；6th gradient temperature is preferably-30 DEG C ~-72 DEG C；
In the gradient temperature of the freezing processing to Chinese catalpa leaf, the first gradient temperature is preferably 4 DEG C, as a control group；Second gradient temperature is preferably-2 DEG C ~-3 DEG C；3rd gradient temperature is preferably-5 DEG C ~-6 DEG C；4th gradient temperature is preferably-8 DEG C ~-10 DEG C；5th gradient temperature is preferably-12 DEG C ~-15 DEG C；6th gradient temperature is preferably-18 DEG C ~-25 DEG C；7th gradient temperature is preferably-25 DEG C ~-35 DEG C.
In the present invention, freezing processing uses the mode of cooling, and thermograde reduces successively；The speed of described cooling is preferably 4 DEG C/h ~ 8 DEG C/h, more preferably 5 DEG C/h ~ 7 DEG C/h, most preferably 6 DEG C/h.
After freezing processing, the present invention carries out electrical impedance mensuration after the Chinese catalpa stem after freezing processing and lyolysis being frozen, the present invention is preferably according to the method described in technique scheme, obtain and Chinese catalpa stem and the electrical impedance parameter of leaf soluble sugar content pole significant correlation, concrete, it is high-frequency electrical conductance with the electrical impedance parameter of Chinese catalpa stem soluble sugar content pole significant correlation, is distribution of relaxation times coefficient with the electrical impedance parameter of Chinese catalpa leaf soluble sugar content pole significant correlation.Chinese catalpa stem after freezing processing and leaf are preferably placed under the temperature conditions of 2 DEG C ~ 10 DEG C and stand by the present invention, more preferably 4 DEG C ~ 6 DEG C；The time of described standing is preferably 20 h ~ 40 h, more preferably 22 h ~ 30 h, most preferably 24 h ~ 27 h.
Obtain with the electrical impedance parameter of Chinese catalpa stem and leaf soluble sugar content pole significant correlation after, the present invention according to described with soluble sugar content dependency extremely significant electrical impedance parameter, obtained the winter resistance of Chinese catalpa stem and leaf by Logistic Equation for Calculating.In the present invention, described Logistic equation sees Formula V:
In Formula V, y is EIS parameter；
xFor freezing processing temperature, unit is DEG C；
EIS is not by EIS parameter value during freeze injury and by the difference of EIS parameter value during freeze injury；
B is the temperature flex point slope of curve, i.e. the slope that EIS parameter changes with cryogenic temperature；
C is inflection temperature, temperature value when i.e. freeze injury rate of change (dy/dx) reaches maximum, for the winter resistance of estimation；
D is by the minima of EIS parameter during freeze injury；
In the present invention, whenxDuring → ∞,y =D, the lower asymptote of function, represent by the minima of EIS parameter during freeze injury；Whenx During → ∞,y=EIS+D, the upper asymptote of function, represent not by the basic value of EIS parameter during freeze injury.Application SPSS13.0 software represents the semilethal low temperature (LT of tissue by the inflection temperature (C value) of accounting equation50), estimate winter resistance.
The invention provides a kind of electrical impedance method and measure the method for soluble sugar content in Chinese catalpa stem and leaf, comprise the following steps: the Electrical Impedance Spectroscopy of detection Chinese catalpa stem and leaf testing sample；Electrical Impedance Spectroscopy according to described testing sample determines applicable equivalent circuit；According to the described equivalent circuit determined, the Electrical Impedance Spectroscopy of described testing sample is carried out data matching, obtains the electrical impedance parameter of described testing sample；According to predetermined Chinese catalpa stem and leaf electrical impedance parameter and the regression model of soluble sugar content and the electrical impedance parameter of described testing sample, obtain soluble sugar content in Chinese catalpa stem and leaf.The method that the present invention provides establishes the regression model between Chinese catalpa stem and the electrical impedance parameter of leaf and its soluble sugar content, thus be calculated the soluble sugar content in Chinese catalpa stem and leaf according to the electrical impedance parameter recorded, and the content being capable of soluble sugar is quick, real-time diagnosis.And, the present invention has also set up the relation between electrical impedance parameter and winter resistance change such that it is able to learn the winter resistance of Chinese catalpa stem and leaf according to the change of electrical impedance parameter, and the estimation for winter resistance change provides important theoretical foundation and technical support.
In order to further illustrate the present invention, it is described in detail below in conjunction with soluble sugar content in the electrical impedance method mensuration tree stem and leaf that the present invention is provided by embodiment and the cold resistant method of Chinese catalpa, but they can not be interpreted as limiting the scope of the present invention.
The test of following embodiment is carried out in January, 2010 in JIUYUE, 2009, material to be tested is 1 year raw grafting, material is the spun gold Chinese catalpa (34 ° of 59 ＇ E of 119 ° of 16 ＇ N) of Lianyungang of Jiangsu, on April 28th, 2009, it is transplanted to Agricultural University Of Hebei's living collection (Baoding, Hebei province, 38 ° of 50 ＇ N, 115 ° of 26 ＇ E), during treating that Chinese catalpa enters Cold Acclimation, every other month stem is sampled on January 20th, 20 days 1 in JIUYUE in 2009, within 20th, every 15 d, leaf is sampled in JIUYUE in 2009, until fallen leaves.
Embodiment 1
Stem is washed with deionized water after only drying and is respectively charged into plastic bag, repeat, bag sprays into a small amount of deionized water, prevents material supercool for 8 times.Freezing processing temperature arranges 7 thermogrades (seeing table 1), with 4 DEG C for comparison, wherein comprises sample and all survives and the most dead temperature.Rate of temperature fall is 6 DEG C of h-1, keep 4 h when arriving treatment temperature, be then placed in 4 DEG C of 24 h that thaw, to be measured.
In table 1 embodiment of the present invention 1, spun gold Chinese catalpa stem Measurement of Cold Resistance freezing processing arranges temperature
Randomly select the stem of 8 section of 15 mm length after defrosting and carry out electrical impedance mensuration:
EIS measures with impedance instrument (HP4284A, USA), test employing Ag/AgCl electrode, adds electrode gel, makes cross section and the gel contacts of sample, measure the Electrical Impedance Spectroscopy obtaining spun gold Chinese catalpa stem on electrode.
Result is as shown in Figure 1, Fig. 1 is the Electrical Impedance Spectroscopy change curve of the spun gold Chinese catalpa stem that the embodiment of the present invention 1 obtains, collection of illustrative plates is for processing data (from right to left, from 80 Hz to 1 MHz totally 42 frequencies) meansigma methods, as seen from Figure 1, during Cold Acclimation, the EIS of spun gold Chinese catalpa stem there occurs change, shows that the internal structure of stem and Physiology and biochemistry there occurs corresponding change to adapt to environmental change.The collection of illustrative plates of stem shows as single arc, and along with the process of Cold Acclimation, the arc of stem is being gradually increased, and its arc top reactance value, in reducing trend always, minimized value-96.86KW January 20.
Embodiment 2
Leaf is washed with deionized water after only drying and is respectively charged into plastic bag, repeat, bag sprays into a small amount of deionized water, prevents material supercool for 8 times.Freezing processing temperature arranges 7 thermogrades (table 2), with 4 DEG C for comparison, wherein comprises sample and all survives and the most dead temperature.Rate of temperature fall is 6 DEG C of h-1, keep 4 h when arriving treatment temperature, be then placed in 4 DEG C of 24 h that thaw, to be measured.
In table 2 embodiment of the present invention 2, spun gold Folium catalpae bungei Measurement of Cold Resistance freezing processing arranges temperature
Randomly select 8 sections after the defrosting rectangle leaf sections without vein, a size of 4 mm × 10 mm, carry out electrical impedance mensuration:
EIS measures with impedance instrument (HP4284A, USA), test employing Ag/AgCl electrode, adds electrode gel, makes cross section and the gel contacts of sample, measure the Electrical Impedance Spectroscopy obtaining spun gold Folium catalpae bungei on electrode.
The electrical impedance of leaf changes as shown in Figure 2, Fig. 2 is the Electrical Impedance Spectroscopy change curve of the spun gold Folium catalpae bungei that the embodiment of the present invention 2 obtains, as seen from Figure 2, during Cold Acclimation, the EIS of spun gold Folium catalpae bungei there occurs change, shows that the internal structure of leaf and Physiology and biochemistry there occurs corresponding change to adapt to environmental change.The collection of illustrative plates of stem shows as single arc, and trend in first increases and then decreases during Cold Acclimation, its arc top reactance value shows as first reducing the trend increased afterwards, and minima occurs in October 5, reaches-482.38 KW.Compared with the Electrical Impedance Spectroscopy of the stem that embodiment 1 obtains, the reactance value of leaf is always below the reactance value of stem, shows that spun gold Chinese catalpa is in order to resist low temperature during Cold Acclimation, and self is regulated, but due to the difference of organ dysfunction, thus cause plant organ reactance value that difference occurs.
Embodiment 3
The stem of spun gold Chinese catalpa is washed with deionized water after only drying, puts into drying in oven 2 d of 80 DEG C, after taking-up, use the content of anthrone determination of color wherein soluble sugar；
Measure 75 groups of samples to spun gold Chinese catalpa stem according to the scheme of embodiment 1 and carry out electrical impedance mensuration, obtain the impedance value of 75 groups of spun gold Chinese catalpa stem samples, refer again to (the Meng Yu such as Meng Yu, Di Bao, Zhang Gang, Feng Xinguo, Xu Chengli, Tian Jun. Betula platyphylla Suk. root system soluble sugar and starch content and the correlation analysis of electrical impedance under water logging stress. Acta Biophysica Sinica, 2013,29 (6): 450 ~ 460.) method carries out the mensuration of EIS parameter, utilizes LEVM 8.06 software, use single-DCE model to carry out data matching, finally give alternating-current resistance rater(Ω m), low-frequency resistance rater 1(Ω m), Extracellular resistancer e(Ω m), intracellular resistancer i(Ω m), relaxation timeτWith distribution of relaxation times coefficientψ6 EIS parameters.
Using SPASS13.0 statistics and Excel 2003 to carry out data analysis, significance of difference analysis uses multiplicity component analysis method；The accuracy of appraising model and the suitability use the general coefficient of determination (R 2), root-mean-square error (RMSE) and 3 index evaluations of relative error (RE), and the degree of fitting of appraising model and reliability.
Utilizing the characteristic variable of EIS parameter obtained and soluble sugar content obtained above to carry out correlation analysis, result is as shown in table 3, table 3 is the embodiment of the present invention 3 and embodiment 5 obtains Cold Acclimation period EIS parameter and soluble sugar content dependency.
The EIS parameter of spun gold Chinese catalpa stem and leaf and soluble sugar content dependency during the Cold Acclimation that table 3 embodiment of the present invention 3 and embodiment 5 obtain
Note: sample size n=75 of stem, sample size n=72 of leaf；
* representing notable in 0.05 level, * * represents the most notable in 0.01 level.
As can be seen from Table 3, during Cold Acclimation, the EIS parameter of spun gold Chinese catalpa stem and soluble sugar content have preferable dependency, wherein the alternating-current resistance rate of stemrWith soluble sugar content pole significant correlation, correlation coefficient is 0.93；
Model with 50 groups of samples, withrFor independent variable, with the soluble sugar content of stem as dependent variable, carry out linear processes regression analysis, simultaneously according to maximum R2Preferably principle chooses optimal appraising model, as shown in table 4, and spun gold Chinese catalpa stem and the soluble sugar of leaf and EIS Partial Linear Models during the Cold Acclimation that table 4 be the embodiment of the present invention 3 and embodiment 5 obtains.
The soluble sugar of spun gold Chinese catalpa stem and leaf and EIS Partial Linear Models during the Cold Acclimation that table 4 embodiment of the present invention 3 and embodiment 5 obtain
Note: sample size n=50 of stem, sample size n=48 of leaf.
In the appraising model obtained, the best-estimated model of stem is yStem=
-0.0177r 2 + 0.889r+ 8.5912 and yStem= -0.0012r 3
+ 0.0316r 2 + 0.351r+ 9.6032, the coefficient of determination is respectively 0.92 and 0.93.
Embodiment 4
Take the testing sample of 25 groups of spun gold Chinese catalpa stems, measure according to the method in embodiment 3 and obtain its EIS parameter alternating-current resistance rater, according to the regression model of the stem that embodiment 3 obtains, it is calculated soluble sugar content in the testing sample of 25 groups of stems；
Use the content of soluble sugar in the testing sample of 25 groups of stems of anthrone determination of color again, for testing model precision.Result is as shown in table 5, table 5 be the embodiment of the present invention 4 and embodiment 6 obtains spun gold Chinese catalpa stem and leaf soluble sugar content measured value and predictive value matching deliberated index.
Spun gold Chinese catalpa stem that table 5 embodiment of the present invention 4 and embodiment 6 obtain and leaf soluble sugar content measured value and predictive value matching deliberated index
As can be seen from Table 5, two regression models of the soluble sugar of the stem that the method that the present invention provides obtains, the coefficient of determination is respectively 0.87 and 0.95, wherein yStem = -0.0177r 2 + 0.889r+ 8.5912 models have less root-mean-square error (RWSE=1.57) and less relative error (RE=8.38%), and checking shows as best model, and the precision of prediction of this model is 91.62%.
Embodiment 5
The leaf of spun gold Chinese catalpa is washed with deionized water after only drying, after putting into the drying in oven 2 d taking-up of 80 DEG C, uses the content of anthrone determination of color wherein soluble sugar；
According to the technical scheme of embodiment 2,72 groups of samples of spun gold Folium catalpae bungei are carried out electrical impedance mensuration, obtain the impedance value of 72 groups of spun gold Folium catalpae bungei samples, carry out data matching according still further to the scheme in embodiment 3, obtain alternating-current resistance rater(Ω m), low-frequency resistance rater 1(Ω m), Extracellular resistancer e(Ω m), intracellular resistancer i(Ω m), relaxation timeτWith distribution of relaxation times coefficientψ6 EIS parameters.
Using SPASS13.0 statistics and Excel 2003 to carry out data analysis, significance of difference analysis uses multiplicity component analysis method；The accuracy of appraising model and the suitability use the general coefficient of determination (R 2), root-mean-square error (RMSE) and 3 index evaluations of relative error (RE), and the degree of fitting of appraising model and reliability.
The characteristic variable utilizing the EIS parameter of the spun gold Folium catalpae bungei obtained carries out correlation analysis with soluble sugar content obtained above, result is as shown in table 3, the EIS parameter of spun gold Chinese catalpa stem and leaf and soluble sugar content dependency during the Cold Acclimation that table 3 is the embodiment of the present invention 3 and embodiment 5 obtains.
As can be seen from Table 3, during Cold Acclimation, the EIS parameter of spun gold Folium catalpae bungei and soluble sugar content have preferable dependency, wherein, the distribution of relaxation times coefficient of leafψNegative correlation extremely notable with soluble sugar content, correlation coefficient is-0.79；
48 groups of data that leaf measures are used for modeling, withψFor independent variable, with the soluble sugar content of leaf as dependent variable, carry out linear processes regression analysis, simultaneously basisR 2Preferably principle chooses optimal appraising model, as shown in table 4, and spun gold Chinese catalpa stem and the soluble sugar of leaf and EIS Partial Linear Models during the Cold Acclimation that table 4 be the embodiment of the present invention 3 and embodiment 5 obtains.
In the appraising model obtained, the best-estimated model of leaf is yLeaf=0.0499ψ 2 - 1.6055ψ+ 27.742 and yLeaf=0.0068ψ 3 -
0.2023ψ 2 + 1.2192ψ+ 18.486, the coefficient of determination is respectively 0.67 and 0.70.
Embodiment 6
Take the testing sample of 24 groups of spun gold Folium catalpae bungei, measure its EIS parameter distribution of relaxation times coefficientψ, according to the appraising model of the leaf that embodiment 5 obtains, it is calculated soluble sugar content in the testing sample of 24 groups of leaves；
Use the content of soluble sugar in the testing sample of 24 groups of leaves of anthrone determination of color again, for the precision of detection model.Result is as shown in table 5, table 5 be the embodiment of the present invention 4 and embodiment 6 obtains spun gold Chinese catalpa stem and leaf soluble sugar content measured value and predictive value matching deliberated index.
As can be seen from Table 5, two regression models of the soluble sugar of the spun gold Folium catalpae bungei that the method that the present invention provides obtains, the coefficient of determination is respectively 0.86 and 0.75, wherein y=0.0068ψ 3 - 0.2023ψ 2 + 1.2192ψ+ 18.486 models have less root-mean-square error (RWSE=3.38) and less relative error (RE=18.94%), and checking shows as best model, and the precision of prediction of this model is 81.06%.
Embodiment 7
Arranging temperature according to the freezing processing in table 1, spun gold Chinese catalpa stem is carried out freezing processing, the regression model obtained according to embodiment 4 after freezing processing is calculated the soluble sugar content in spun gold Chinese catalpa stem.
Measurement result is as shown in Figure 3, Fig. 3 is the change curve of soluble sugar content in spun gold Chinese catalpa stem during the Cold Acclimation that the embodiment of the present invention 7 obtains, as seen from Figure 3, during Cold Acclimation, spun gold Chinese catalpa stem soluble sugar content is in rising trend, adds 98.47% than initial stage at Cold Acclimation soluble sugar content in latter stage.
Embodiment 8
Arranging temperature according to the freezing in table 2, spun gold Folium catalpae bungei is carried out freezing processing, the regression model obtained according to embodiment 6 after freezing processing is calculated the soluble sugar content in spun gold Folium catalpae bungei.
Measurement result is as shown in Figure 4, Fig. 4 is the change curve of soluble sugar content in spun gold Folium catalpae bungei during the Cold Acclimation that the embodiment of the present invention 8 obtains, as seen from Figure 4, during Cold Acclimation, spun gold Folium catalpae bungei soluble sugar content is in rising trend, adds 57.90% than initial stage at Cold Acclimation soluble sugar content in latter stage.
Embodiment 9
The spun gold Chinese catalpa stem that freezing processing is crossed be placed in 4 DEG C of environment place 24 h thaw after, measure its relative conductivity (EL) and EIS, relative conductivity test process particularly as follows:
Each spun gold Chinese catalpa stem testing sample is washed with deionized water only, is averagely split into 4 lobes and puts in the test tube filling 12 mL deionized waters, each sample replication 4 times, averaged；
The test tube Parafilm film filling sample is sealed, puts into and shaking table shakes 24 h, measure initial electric conductivity value (C with DDSL-308 type conductivity meter1) and initial blank electric conductivity value (C01)；
Then, test tube put in water-bath boiling and boils 20 min, then putting into shaking table shakes 24 h, measuring final electric conductivity value (C2) and final blank electric conductivity value (C02).With formula VI calculate relative conductivity:
Technical scheme according to embodiment 1 measures the mensuration of the EIS of stem after thawing.The change of relative conductivity represents with cold resistant relation Logistic equation Formula VII with cold resistant relation or electrical impedance parameter:
In EL French:yFor relative conductivity；xFor freezing processing temperature (DEG C)；BFor the temperature flex point slope of curve, relative conductivity is with the slope of the time of process change；C is inflection temperature, freeze injury rate of change (d y / d x ) temperature value when reaching maximum, it is the winter resistance of estimation.As x → ∞,y=D, for the lower asymptote of function, represent not by the freeze injury phase basic value to electrical conductivity.As x → ∞,y=A +D, for the upper asymptote of function, represent by the freeze injury phase maximum to electrical conductivity.
In EIS French:y=EIS parameter；As x → ∞,y =D, the lower asymptote of function, represent by the minima of EIS parameter during freeze injury；As x → ∞,y=A +D, the upper asymptote of function, represent not by the basic value of EIS parameter during freeze injury.Application SPSS13.0 software represents the semilethal low temperature (LT of tissue by the inflection temperature (C value) of accounting equation50), estimate winter resistance.
Result is as shown in table 6, table 6 is the embodiment of the present invention 9 and embodiment 10 obtains Cold Acclimation period EL method and EIS parametric measurement spun gold Chinese catalpa stem and the winter resistance result of leaf.
Cold Acclimation period EL method that table 6 embodiment of the present invention 9 and embodiment 10 obtain and EIS parametric measurement spun gold Chinese catalpa stem and the winter resistance result of leaf
Cell leakage plant identification winter resistance result is more consistent at variable rate technology with it.Use EL method and the winter resistance of optimal EIS parametric measurement spun gold Chinese catalpa stem chosen, by Logistic Equation for Calculating winter resistance, as shown in Table 6, the cold resistant value of stem that EL method measures withrThe cold resistant value difference of estimation stem is different not quite, and its mean absolute error value is 3.01.
Embodiment 10
The spun gold Folium catalpae bungei that freezing processing is crossed be placed in 4 DEG C of environment place 24 h thaw after, measure its relative conductivity and EIS, relative conductivity test process particularly as follows:
Beat with the card punch of a diameter of 5 mm and take 4 pieces of leaf disks, obtain 4 testing samples are placed in the test tube filling 12 mL deionized waters, each sample replication 4 times；
The test tube Parafilm film filling sample is sealed, put into and shaking table shakes 24 h, according to the method in embodiment 9, use EL method and the winter resistance of EIS method estimation spun gold Folium catalpae bungei, result is as shown in table 6, table 6 is the embodiment of the present invention 9 and embodiment 10 obtains Cold Acclimation period EL method and EIS parametric measurement spun gold Chinese catalpa stem and the winter resistance result of leaf.
Cell leakage plant identification winter resistance result is more consistent at variable rate technology with it.Use EL method and the optimal EIS parametric measurement spun gold Chinese catalpa stem chosen and leaf winter resistance, by Logistic Equation for Calculating winter resistance, as shown in Table 6, the cold resistant value of leaf that EL method measures withψThe cold resistant value difference of estimation leaf is different not quite, and its mean absolute error value is 2.83.
As seen from the above embodiment, the invention provides a kind of electrical impedance and measure the method for soluble sugar content in Chinese catalpa stem and leaf, comprise the following steps: the Electrical Impedance Spectroscopy of detection Chinese catalpa stem and leaf testing sample；Electrical Impedance Spectroscopy according to described testing sample determines applicable equivalent circuit；According to the described equivalent circuit determined, the Electrical Impedance Spectroscopy of described testing sample is carried out data matching, obtains the electrical impedance parameter of described testing sample；According to predetermined Chinese catalpa stem and leaf electrical impedance parameter and the regression model of soluble sugar content and the electrical impedance parameter of described testing sample, obtain soluble sugar content in Chinese catalpa stem and leaf.The method that the present invention provides establishes the regression model between Chinese catalpa stem and the electrical impedance parameter of leaf and its soluble sugar content, thus be calculated the soluble sugar content in Chinese catalpa stem and leaf according to the electrical impedance parameter recorded, and the content being capable of soluble sugar is quick, real-time diagnosis.And, the present invention has also set up the relation between electrical impedance parameter and winter resistance change such that it is able to learn the winter resistance of Chinese catalpa stem and leaf according to the change of electrical impedance parameter, and the estimation for winter resistance change provides important theoretical foundation and technical support.
The above is only the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (10)
1. a method for soluble sugar content during electrical impedance method measures Chinese catalpa stem and leaf, comprises the following steps:
The Electrical Impedance Spectroscopy of detection Chinese catalpa stem and leaf testing sample；
Electrical Impedance Spectroscopy according to described testing sample determines applicable equivalent circuit；
According to the described equivalent circuit determined, the Electrical Impedance Spectroscopy of described testing sample is carried out data matching, obtains the electrical impedance parameter of described testing sample；
According to predetermined Chinese catalpa stem and leaf electrical impedance parameter and the regression model of soluble sugar content and the electrical impedance parameter of described testing sample, obtain soluble sugar content in Chinese catalpa stem and leaf.
Assay method the most according to claim 1, it is characterised in that described electrical impedance parameter includes alternating-current resistance rate, low-frequency resistance rate, Extracellular resistance, intracellular resistance, relaxation time and distribution of relaxation times coefficient.
Assay method the most according to claim 1, it is characterised in that described equivalent circuit is single-DCE model.
Assay method the most according to claim 1, it is characterised in that the alternating-current resistance rate that regression model is Chinese catalpa stem of described Chinese catalpa stem electrical impedance parameter and soluble sugar content and the regression model of soluble sugar content.
Assay method the most according to claim 4, it is characterised in that the alternating-current resistance rate of described Chinese catalpa stem is independent variable with the alternating-current resistance rate of Chinese catalpa stem in the regression model of soluble sugar content, and soluble sugar content is dependent variable.
Assay method the most according to claim 5, it is characterised in that the alternating-current resistance rate of described Chinese catalpa stem and the regression model of soluble sugar content are quadratic equation or cubic equation.
Assay method the most according to claim 1, it is characterised in that the distribution of relaxation times coefficient that regression model is Chinese catalpa leaf of described Chinese catalpa leaf electrical impedance parameter and soluble sugar content and the regression model of soluble sugar content.
Assay method the most according to claim 7, it is characterised in that the distribution of relaxation times coefficient of described Chinese catalpa leaf is independent variable with the distribution of relaxation times coefficient of Chinese catalpa leaf in the regression model of soluble sugar content, and soluble sugar content is dependent variable.
Assay method the most according to claim 8, it is characterised in that the distribution of relaxation times coefficient of described Chinese catalpa leaf and the regression model of soluble sugar content are quadratic equation or cubic equation.
10. electrical impedance method measures the cold resistant method of Chinese catalpa, comprises the following steps:
Detection Chinese catalpa stem and the Electrical Impedance Spectroscopy of leaf testing sample；
Electrical Impedance Spectroscopy according to described testing sample determines suitable equivalent circuit；
According to the described equivalent circuit determined, the Electrical Impedance Spectroscopy of described testing sample is carried out data matching, obtains the electrical impedance parameter of described testing sample；
The soluble sugar content of predetermined Chinese catalpa stem and leaf and the electrical impedance parameter of described testing sample are carried out correlation analysis, obtains and soluble sugar content dependency extremely significantly electrical impedance parameter；
According to described electrical impedance parameter extremely significant with soluble sugar content dependency, obtained the winter resistance of Chinese catalpa stem and leaf by Logistic Equation for Calculating.
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