CN105798324A - Mimic enzyme based on self-assembly structure as well as preparation method and application thereof - Google Patents
Mimic enzyme based on self-assembly structure as well as preparation method and application thereof Download PDFInfo
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- CN105798324A CN105798324A CN201610165756.0A CN201610165756A CN105798324A CN 105798324 A CN105798324 A CN 105798324A CN 201610165756 A CN201610165756 A CN 201610165756A CN 105798324 A CN105798324 A CN 105798324A
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- tripolycyanamide
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- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 238000001338 self-assembly Methods 0.000 title claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 7
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 7
- 230000003278 mimic effect Effects 0.000 title abstract 3
- 239000000126 substance Substances 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000003638 chemical reducing agent Substances 0.000 claims abstract description 9
- 229910052751 metal Inorganic materials 0.000 claims abstract description 9
- 239000002184 metal Substances 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 7
- 238000011161 development Methods 0.000 claims abstract description 4
- 239000012266 salt solution Substances 0.000 claims abstract description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 21
- 238000006243 chemical reaction Methods 0.000 claims description 11
- QMKYBPDZANOJGF-UHFFFAOYSA-N benzene-1,3,5-tricarboxylic acid Chemical compound OC(=O)C1=CC(C(O)=O)=CC(C(O)=O)=C1 QMKYBPDZANOJGF-UHFFFAOYSA-N 0.000 claims description 10
- 238000012360 testing method Methods 0.000 claims description 10
- ZFSLODLOARCGLH-UHFFFAOYSA-N isocyanuric acid Chemical compound OC1=NC(O)=NC(O)=N1 ZFSLODLOARCGLH-UHFFFAOYSA-N 0.000 claims description 9
- 150000003839 salts Chemical class 0.000 claims description 9
- FDWREHZXQUYJFJ-UHFFFAOYSA-M gold monochloride Chemical compound [Cl-].[Au+] FDWREHZXQUYJFJ-UHFFFAOYSA-M 0.000 claims description 6
- 239000001509 sodium citrate Substances 0.000 claims description 6
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 6
- AFSDNFLWKVMVRB-UHFFFAOYSA-N Ellagic acid Chemical compound OC1=C(O)C(OC2=O)=C3C4=C2C=C(O)C(O)=C4OC(=O)C3=C1 AFSDNFLWKVMVRB-UHFFFAOYSA-N 0.000 claims description 5
- ATJXMQHAMYVHRX-CPCISQLKSA-N Ellagic acid Natural products OC1=C(O)[C@H]2OC(=O)c3cc(O)c(O)c4OC(=O)C(=C1)[C@H]2c34 ATJXMQHAMYVHRX-CPCISQLKSA-N 0.000 claims description 5
- 229920002079 Ellagic acid Polymers 0.000 claims description 5
- 229960002852 ellagic acid Drugs 0.000 claims description 5
- 235000004132 ellagic acid Nutrition 0.000 claims description 5
- FAARLWTXUUQFSN-UHFFFAOYSA-N methylellagic acid Natural products O1C(=O)C2=CC(O)=C(O)C3=C2C2=C1C(OC)=C(O)C=C2C(=O)O3 FAARLWTXUUQFSN-UHFFFAOYSA-N 0.000 claims description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 4
- XXAXVMUWHZHZMJ-UHFFFAOYSA-N Chymopapain Chemical compound OC1=CC(S(O)(=O)=O)=CC(S(O)(=O)=O)=C1O XXAXVMUWHZHZMJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- VCJMYUPGQJHHFU-UHFFFAOYSA-N iron(3+);trinitrate Chemical compound [Fe+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O VCJMYUPGQJHHFU-UHFFFAOYSA-N 0.000 claims description 4
- GPNDARIEYHPYAY-UHFFFAOYSA-N palladium(ii) nitrate Chemical compound [Pd+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O GPNDARIEYHPYAY-UHFFFAOYSA-N 0.000 claims description 4
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 3
- WZRRRFSJFQTGGB-UHFFFAOYSA-N 1,3,5-triazinane-2,4,6-trithione Chemical compound S=C1NC(=S)NC(=S)N1 WZRRRFSJFQTGGB-UHFFFAOYSA-N 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- 235000010323 ascorbic acid Nutrition 0.000 claims description 2
- 229960005070 ascorbic acid Drugs 0.000 claims description 2
- 239000011668 ascorbic acid Substances 0.000 claims description 2
- 239000003054 catalyst Substances 0.000 claims description 2
- 229960004106 citric acid Drugs 0.000 claims description 2
- 235000015165 citric acid Nutrition 0.000 claims description 2
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 2
- UFMZWBIQTDUYBN-UHFFFAOYSA-N cobalt dinitrate Chemical compound [Co+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O UFMZWBIQTDUYBN-UHFFFAOYSA-N 0.000 claims description 2
- 229910001981 cobalt nitrate Inorganic materials 0.000 claims description 2
- 229940097267 cobaltous chloride Drugs 0.000 claims description 2
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 2
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 claims description 2
- XTVVROIMIGLXTD-UHFFFAOYSA-N copper(II) nitrate Chemical compound [Cu+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O XTVVROIMIGLXTD-UHFFFAOYSA-N 0.000 claims description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 2
- OPQARKPSCNTWTJ-UHFFFAOYSA-L copper(ii) acetate Chemical compound [Cu+2].CC([O-])=O.CC([O-])=O OPQARKPSCNTWTJ-UHFFFAOYSA-L 0.000 claims description 2
- 238000004090 dissolution Methods 0.000 claims description 2
- 238000004043 dyeing Methods 0.000 claims description 2
- 239000003344 environmental pollutant Substances 0.000 claims description 2
- 229960002089 ferrous chloride Drugs 0.000 claims description 2
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 claims description 2
- NMCUIPGRVMDVDB-UHFFFAOYSA-L iron dichloride Chemical compound Cl[Fe]Cl NMCUIPGRVMDVDB-UHFFFAOYSA-L 0.000 claims description 2
- 229910000358 iron sulfate Inorganic materials 0.000 claims description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 claims description 2
- KBJMLQFLOWQJNF-UHFFFAOYSA-N nickel(ii) nitrate Chemical compound [Ni+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O KBJMLQFLOWQJNF-UHFFFAOYSA-N 0.000 claims description 2
- PIBWKRNGBLPSSY-UHFFFAOYSA-L palladium(II) chloride Chemical compound Cl[Pd]Cl PIBWKRNGBLPSSY-UHFFFAOYSA-L 0.000 claims description 2
- CLSUSRZJUQMOHH-UHFFFAOYSA-L platinum dichloride Chemical compound Cl[Pt]Cl CLSUSRZJUQMOHH-UHFFFAOYSA-L 0.000 claims description 2
- 231100000719 pollutant Toxicity 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 229910001961 silver nitrate Inorganic materials 0.000 claims description 2
- 239000012279 sodium borohydride Substances 0.000 claims description 2
- 229910000033 sodium borohydride Inorganic materials 0.000 claims description 2
- 229960001790 sodium citrate Drugs 0.000 claims description 2
- 235000011083 sodium citrates Nutrition 0.000 claims description 2
- 238000003018 immunoassay Methods 0.000 claims 1
- 239000007864 aqueous solution Substances 0.000 abstract description 14
- 238000006555 catalytic reaction Methods 0.000 abstract description 11
- 102000003992 Peroxidases Human genes 0.000 abstract description 10
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 6
- 239000002082 metal nanoparticle Substances 0.000 abstract description 6
- 239000000243 solution Substances 0.000 abstract description 4
- 230000015572 biosynthetic process Effects 0.000 abstract description 3
- 238000003786 synthesis reaction Methods 0.000 abstract description 3
- 238000011065 in-situ storage Methods 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 150000002500 ions Chemical class 0.000 abstract 1
- -1 salt ions Chemical class 0.000 abstract 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 18
- 239000010931 gold Substances 0.000 description 18
- 229910052737 gold Inorganic materials 0.000 description 18
- 230000003197 catalytic effect Effects 0.000 description 7
- 239000002086 nanomaterial Substances 0.000 description 5
- 150000002343 gold Chemical class 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- QKUSRAKPUWQSJS-UHFFFAOYSA-N diazanium 3-ethyl-2H-1,3-benzothiazole-6-sulfonate Chemical class [NH4+].[NH4+].[O-]S(=O)(=O)C1=CC=C2N(CC)CSC2=C1.[O-]S(=O)(=O)C1=CC=C2N(CC)CSC2=C1 QKUSRAKPUWQSJS-UHFFFAOYSA-N 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 108010015776 Glucose oxidase Proteins 0.000 description 2
- 239000004366 Glucose oxidase Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 2
- 229940116332 glucose oxidase Drugs 0.000 description 2
- 235000019420 glucose oxidase Nutrition 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical class [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- CETPSERCERDGAM-UHFFFAOYSA-N ceric oxide Chemical compound O=[Ce]=O CETPSERCERDGAM-UHFFFAOYSA-N 0.000 description 1
- 229910000422 cerium(IV) oxide Inorganic materials 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/05—Metallic powder characterised by the size or surface area of the particles
- B22F1/054—Nanosized particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Nanotechnology (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Composite Materials (AREA)
- Manufacturing & Machinery (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a preparation method for a mimic enzyme based on a self-assembly structure. The preparation method comprises the following steps: (1) dissolving chemical substances capable of carrying out a self-assembly behaviour into water; (2) adding an active metal salt solution and a reducer in the solution in the step (1), and stirring to obtain the mimic enzyme based on the self-assembly structure. In the preparation method provided by the invention, two previously prepared chemical substance aqueous solutions capable of carrying out the self-assembly behaviour are mixed, two types of molecules are assembled together through an action force among weak molecules, and metal salt ions are adsorbed on the surfaces of the molecules and generate metal nanoparticles in situ under the action of the reducer; and the obtained metal nanoparticles based on the self-assembly structure have a great peroxidase activity under the conditions of wide pH and temperature ranges, high ion strength and the like. The synthesis conditions of the material are moderate, simple and convenient, the price of raw materials is low, and business development is facilitated. The metal nanoparticle material based on the self-assembly structure, which is disclosed by the invention, can also be applied to many fields of electro-catalysis, water treatment, biomedicine and the like, and has a wide application prospect.
Description
Technical field
The present invention relates to macromolecular compound field, specifically disclose a kind of based on self-assembled structures analogue enztme and preparation method thereof and application.
Background technology
Compared with protease, although nano material analogue enztme has good acid, alkali and high-temperature stability, with low cost, synthesis is simple, can prepare in a large number, it is easy to by types of functionality molecular modification and labelling, but still suffer from very big gap, and facing challenges is still very severe.Common nano material analogue enztme Fe3O4, CeO2, nanometer gold analog derivative, Graphene analog derivative etc. all can be used to combine specific oxidase (such as glucoseoxidase etc.) and developer (such as 3,3', 5,5'-tetramethyl benzidine TMB etc.) carry out the detection of specific molecular.Although achieving certain Detection results, but it is difficult to be commercially exploited and is applied in daily life, its main cause has: the common high catalytic activity condition of a) easily prepared nano material analogue enztme is at sour environment, almost without catalytic effect under neutrallty condition, and native enzyme (such as glucoseoxidase etc.) has good catalytic effect in neutral conditions, under acid condition, protease may inactivate, and is difficult to associating protease and is applied to the exploitation of test kit and the development of reagent paper.Although having been reported title to prepare the nano material analogue enztme that can play high catalytic activity in neutral conditions, but its preparation condition harshness being difficult to be commercially employed；B) catalytic reaction activity is low, although some nano material analogue enztmes can be used to enzyme linked immunological labelling, but its catalysis activity still compared with horseradish peroxidase, cannot be difficult to be applied to the exploitation of enzyme linked immunological kit.
Summary of the invention
In order to overcome prior art Problems existing, the present invention provides a kind of based on self-assembled structures analogue enztme and preparation method thereof and application.
Present invention firstly provides a kind of preparation method based on self-assembled structures analogue enztme, comprise the steps:
(1) will can occur the compound dissolution of self assembly behavior in water；
(2) in above-mentioned (1), add active metal salt solution, reducing agent, stirring, obtain described based on self-assembled structures analogue enztme.
Further, the described chemical substance that self assembly behavior can occur is at least one in following substances combination: tripolycyanamide and cyanuric acid, tripolycyanamide and ellagic acid, tripolycyanamide and catechol-3,5-disulfonic acid, tripolycyanamide and citric acid, tripolycyanamide and trimesic acid, tripolycyanamide and trithiocyanuric acid, cyanuric acid and citric acid, cyanuric acid and trimesic acid.
Further, described active metal salt is at least one in following substances: copper sulfate, copper chloride, copper acetate, copper nitrate, silver nitrate, gold chloride, platinum chloride, Palladous chloride., Palladous nitrate., iron chloride, ferric nitrate, ferrous chloride, iron sulfate, cobaltous chloride, cobalt nitrate, nickel nitrate, Nickel dichloride..
Further, described reducing agent is at least one in following substances: sodium borohydride, potassium borohydride, ascorbic acid, sodium citrate, citric acid, ellagic acid, catechol-3,5-disulfonic acid.
Further, the concentration of the described chemicals that self assembly behavior can occur can be 0.01～10mmol/L, concretely 0.01mmol/L, 0.05mmol/L, 0.1mmol/L, 0.5mmol/L, 1mmol/L, 5mmol/L, 10mmol/L, mol ratio can be 1～3:1～3, concretely 1～3:1,1:1 or 3:1.
Further, the concentration of described active metal salt can be 0.01～1mmol/L, concretely 0.01mmol/L, 0.05mmol/L, 0.1mmol/L, 0.5mmol/L, 1mmol/L, concretely 0.1mmol/L, 0.5mmol/L, 1mmol/L, 5mmol/L, 10mmol/L.
Further, the concentration of described reducing agent can be 0.1～10mmol/L.
Further, in described step (2), described mixing time can be 1～48h, concretely 1h, 5h, 10h, 15h, 20h, 25h, 30h, 35h, 40h, 48h；Described reaction temperature can be that in the water of 0～100 DEG C, reaction temperature can be in the water of 0～100 DEG C, concretely 0～100 DEG C, 0 DEG C, 10 DEG C, 20 DEG C, 30 DEG C, 40 DEG C, 50 DEG C, 60 DEG C, 70 DEG C, 80 DEG C or 100 DEG C.
On the other hand, present disclosure also include by said method prepare based on self-assembled structures analogue enztme.
On the other hand, present disclosure also includes described can be used for any one application following based on self-assembled structures analogue enztme material:
1) biomolecule test kit and reagent paper development；
2) biological tissue's dyeing；
3) Pollutant Treatment；
4) eelctro-catalyst.
The invention have the benefit that in preparation method provided by the invention, the chemical substance aqueous solution that self assembly behavior can occur prepared in advance will be mixed, two kinds of molecules are fitted together by Hyarogen-bonding, ionizable metal salt is in its surface adsorption, under the effect of reducing agent, in-situ preparation metal nanoparticle, the obtained metal nanoparticle based on self-assembled structures has good peroxidase activity under wide pH and the condition such as temperature range, high ionic strength.This materials synthesis mild condition is easy, and low in raw material price is easily commercially exploited.The numerous areas such as the metal nanoparticle material based on self-assembled structures of the present invention can also be applied to electro-catalysis, water processes, biomedical, have wide practical use.
In order to be more fully understood that and implement, describe the present invention in detail below in conjunction with accompanying drawing.
Accompanying drawing explanation
Fig. 1 is the transmission electron microscope photo based on self-assembled structures analogue enztme of embodiment 1 preparation.
Fig. 2 be embodiment 1 preparation based on self-assembled structures analogue enztme rate of catalysis reaction under condition of different pH, wherein a, b, c, d, e respectively ph is rate of catalysis reaction when 4,5,6,7,8.
Fig. 3 be embodiment 1 preparation based on self-assembled structures analogue enztme rate of catalysis reaction at different temperatures, wherein A, B, C, D, E, F, G respectively reaction temperature is rate of catalysis reaction when 25,37,45,55,65,75,85 DEG C.
Detailed description of the invention
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all commercially obtain.
[embodiment 1] is based on the preparation of self-assembled structures analogue enztme and Mimetic Peroxidase active testing thereof
By 100mL tripolycyanamide (5mmol/L) aqueous solution and 100mL cyanuric acid (5mmol/L) aqueous solution, adding 4mL gold chloride (10g/L), add 1mL sodium citrate (50mmol/L), stirring at normal temperature 24 hours, obtains the aqueous solution based on self-assembled structures nanometer gold.
1) based on the sign of self-assembled structures nanometer gold:
Detecting with Japan's transmission electron microscope (JEM-2100F) obtains based on self-assembled structures nanometer gold pattern under above-mentioned condition, as it is shown in figure 1, what result showed prepared by the present embodiment is little granule nanometer gold high degree of dispersion shape on carrier based on self-assembled structures nanometer gold pattern.
2) based on the Mimetic Peroxidase determination of activity of self-assembled structures nanometer gold:
That embodiment 1 is prepared takes 0.1g based on self-assembled structures nanometer gold, ultrasonic disperse is in the water of 100mL, take the above-mentioned solution of 0.1mL to join the buffer system of 0.9mL difference pH and (include 0.05mol/LTris-Ac, 1mmol/LH2O2,1mmol/L2,2-azino-two (3-ethyl-benzothiazole-6-sulfonic acid) di-ammonium salts (ABTS)) in, measure based on self-assembled structures nanometer gold catalytic rate in 37 DEG C of different pH systems, as in figure 2 it is shown, there is significantly high rate of catalysis reaction based on self-assembled structures nanometer gold in pH4～8 scope；
That embodiment 1 is prepared takes 0.1g based on self-assembled structures nanometer gold, ultrasonic disperse is in the water of 100mL, take the above-mentioned solution of 0.1mL to join the buffer system of 0.9mLpH=7 and (include 0.05mol/LTris-Ac, 1mmol/LH2O2,1mmol/L2,2-azino-two (3-ethyl-benzothiazole-6-sulfonic acid) di-ammonium salts (ABTS)) in, measure based on self-assembled structures nanometer gold catalytic rate at different temperatures, as it is shown on figure 3, there is significantly high rate of catalysis reaction based on self-assembled structures nanometer gold 25～85 DEG C of scopes；
That embodiment 1 is prepared takes 0.1g based on self-assembled structures nanometer gold, ultrasonic disperse is in the water of 100mL, take the above-mentioned solution of 0.1mL to join the buffer system of 0.9mLpH=7 and (include 0.05mol/LTris-Ac, 1mmol/LH2O2, 1mmol/L2, 2-azino-two (3-ethyl-benzothiazole-6-sulfonic acid) di-ammonium salts (ABTS)) in, measure based on the catalytic rate under the self-assembled structures nanometer gold different NaCl concentration at 37 DEG C of temperature, based on self-assembled structures nanometer gold (5mol/L) under significantly high salt ionic concentration, its rate of catalysis reaction is unaffected.
[embodiment 2] is based on the preparation of self-assembled structures analogue enztme and Mimetic Peroxidase active testing thereof
By 100mL tripolycyanamide (5mmol/L) aqueous solution and 100mL ellagic acid (5mmol/L) aqueous solution, adding 4mL gold chloride (10g/L), add 1mL sodium citrate (50mmol/L), stirring at normal temperature 24 hours, obtains the aqueous solution based on self-assembled structures nanometer gold.
Mimetic Peroxidase active testing based on self-assembled structures nanometer gold: analogue enztme is identical with embodiment 1 in wide pH, temperature, salt ionic concentration scope, and the result of gained is also similar to that embodiment 1.
[embodiment 3] is based on the preparation of self-assembled structures analogue enztme and Mimetic Peroxidase active testing thereof
By 100mL cyanuric acid (5mmol/L) aqueous solution and 100mL citric acid (5mmol/L) aqueous solution, adding 4mL gold chloride (10g/L), add 1mL sodium citrate (50mmol/L), stirring at normal temperature 24 hours, obtains the aqueous solution based on self-assembled structures nanometer gold.
Mimetic Peroxidase active testing based on self-assembled structures nanometer gold: analogue enztme is identical with embodiment 1 in wide pH, temperature, salt ionic concentration scope, and the result of gained is also similar to that embodiment 1.
[embodiment 4] is based on the preparation of self-assembled structures analogue enztme and Mimetic Peroxidase active testing thereof
By 100mL cyanuric acid (5mmol/L) aqueous solution and 100mL trimesic acid (5mmol/L) aqueous solution, adding 4mL gold chloride (10g/L), add 1mL sodium citrate (50mmol/L), stirring at normal temperature 24 hours, obtains the aqueous solution based on self-assembled structures nanometer gold.
Mimetic Peroxidase active testing based on self-assembled structures nanometer gold: analogue enztme is identical with embodiment 1 in wide pH, temperature, salt ionic concentration scope, and the result of gained is also similar to that embodiment 1.
The invention is not limited in above-mentioned embodiment, if to the various changes of the present invention or deformation without departing from the spirit and scope of the present invention, if these are changed and deform within the claim and the equivalent technologies scope that belong to the present invention, then the present invention is also intended to comprise these changes and deformation.
Claims (10)
1., based on a preparation method for self-assembled structures analogue enztme, comprise the steps:
(1) will can occur the chemical dissolution of self assembly behavior in water；
(2) in above-mentioned (1), add active metal salt solution, reducing agent, stirring, obtain described based on self-assembled structures analogue enztme.
2. preparation method according to claim 1, it is characterized in that: the described chemical substance that self assembly behavior can occur is at least one in following substances combination: tripolycyanamide and cyanuric acid, tripolycyanamide and ellagic acid, tripolycyanamide and catechol-3,5-disulfonic acid, tripolycyanamide and citric acid, tripolycyanamide and trimesic acid, tripolycyanamide and trithiocyanuric acid, cyanuric acid and citric acid, cyanuric acid and trimesic acid.
3. preparation method according to claim 2, it is characterised in that: described active metal salt is at least one in following substances: copper sulfate, copper chloride, copper acetate, copper nitrate, silver nitrate, gold chloride, platinum chloride, Palladous chloride., Palladous nitrate., iron chloride, ferric nitrate, ferrous chloride, iron sulfate, cobaltous chloride, cobalt nitrate, nickel nitrate, Nickel dichloride..
4. preparation method according to claim 3, it is characterised in that: described reducing agent is at least one in following substances: sodium borohydride, potassium borohydride, ascorbic acid, sodium citrate, citric acid, ellagic acid, catechol-3,5-disulfonic acid.
5. preparation method according to claim 4, it is characterised in that: the concentration of the described chemicals that self assembly behavior can occur can be 0.01～10mmol/L, and mol ratio can be 1～3:1～3.
6. preparation method according to claim 5, it is characterised in that: the concentration of described active metal salt can be 0.01～1mmol/L.
7. preparation method according to claim 6, it is characterised in that: the concentration of described reducing agent can be 0.1～10mmol/L.
8. preparation method according to claim 7, it is characterised in that: in described step (2), mixing time can be 1～48h, and described reaction temperature can be in the water of 0～100 DEG C.
9. method according to any one of claim 1-8 prepare based on self-assembled structures analogue enztme.
10. described in claim 9 based on self-assembled structures analogue enztme following in any one in application:
1) biomolecule test kit and reagent paper development；
2) enzyme immunoassay；
3) biological tissue's dyeing；
4) Pollutant Treatment；
5) eelctro-catalyst.
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