CN105797703A - Method for preparing zinc oxide photocatalyst with peanut meal protein as biological template - Google Patents
Method for preparing zinc oxide photocatalyst with peanut meal protein as biological template Download PDFInfo
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- CN105797703A CN105797703A CN201610240085.XA CN201610240085A CN105797703A CN 105797703 A CN105797703 A CN 105797703A CN 201610240085 A CN201610240085 A CN 201610240085A CN 105797703 A CN105797703 A CN 105797703A
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- zinc oxide
- arachidis hypogaeae
- semen arachidis
- deionized water
- hypogaeae dregs
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- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 title claims abstract description 57
- 239000011941 photocatalyst Substances 0.000 title claims abstract description 31
- 239000011787 zinc oxide Substances 0.000 title claims abstract description 29
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 21
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 13
- 235000017060 Arachis glabrata Nutrition 0.000 title abstract 6
- 241001553178 Arachis glabrata Species 0.000 title abstract 6
- 235000010777 Arachis hypogaea Nutrition 0.000 title abstract 6
- 235000018262 Arachis monticola Nutrition 0.000 title abstract 6
- 235000012054 meals Nutrition 0.000 title abstract 6
- 235000020232 peanut Nutrition 0.000 title abstract 6
- 239000001963 growth medium Substances 0.000 claims abstract description 14
- 239000000725 suspension Substances 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 238000003837 high-temperature calcination Methods 0.000 claims abstract description 8
- 210000000582 semen Anatomy 0.000 claims description 40
- 239000008367 deionised water Substances 0.000 claims description 30
- 229910021641 deionized water Inorganic materials 0.000 claims description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 239000000843 powder Substances 0.000 claims description 24
- 239000002244 precipitate Substances 0.000 claims description 24
- 238000002156 mixing Methods 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 18
- 238000000227 grinding Methods 0.000 claims description 15
- 238000000855 fermentation Methods 0.000 claims description 13
- 230000004151 fermentation Effects 0.000 claims description 13
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 238000005119 centrifugation Methods 0.000 claims description 12
- 239000007787 solid Substances 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 10
- 239000000047 product Substances 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 7
- XIOUDVJTOYVRTB-UHFFFAOYSA-N 1-(1-adamantyl)-3-aminothiourea Chemical compound C1C(C2)CC3CC2CC1(NC(=S)NN)C3 XIOUDVJTOYVRTB-UHFFFAOYSA-N 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 235000008708 Morus alba Nutrition 0.000 claims description 6
- 240000000249 Morus alba Species 0.000 claims description 6
- 238000001354 calcination Methods 0.000 claims description 6
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 238000007654 immersion Methods 0.000 claims description 6
- 238000009413 insulation Methods 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 239000004570 mortar (masonry) Substances 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 238000010298 pulverizing process Methods 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 6
- ZNCPFRVNHGOPAG-UHFFFAOYSA-L sodium oxalate Chemical compound [Na+].[Na+].[O-]C(=O)C([O-])=O ZNCPFRVNHGOPAG-UHFFFAOYSA-L 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 238000004506 ultrasonic cleaning Methods 0.000 claims description 6
- 238000001291 vacuum drying Methods 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 4
- 230000001699 photocatalysis Effects 0.000 abstract description 10
- 239000002245 particle Substances 0.000 abstract description 5
- 239000003054 catalyst Substances 0.000 abstract description 2
- 239000011148 porous material Substances 0.000 abstract description 2
- 241001537207 Flammulina Species 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
- 230000015556 catabolic process Effects 0.000 description 13
- 238000006731 degradation reaction Methods 0.000 description 13
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 12
- 229960000907 methylthioninium chloride Drugs 0.000 description 12
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 12
- 229940043267 rhodamine b Drugs 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000007935 neutral effect Effects 0.000 description 4
- 238000007146 photocatalysis Methods 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 239000002351 wastewater Substances 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 241001466460 Alveolata Species 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000002957 persistent organic pollutant Substances 0.000 description 2
- 239000004065 semiconductor Substances 0.000 description 2
- 229910010413 TiO 2 Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011858 nanopowder Substances 0.000 description 1
- 238000006862 quantum yield reaction Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J23/00—Catalysts comprising metals or metal oxides or hydroxides, not provided for in group B01J21/00
- B01J23/06—Catalysts comprising metals or metal oxides or hydroxides, not provided for in group B01J21/00 of zinc, cadmium or mercury
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J35/00—Catalysts, in general, characterised by their form or physical properties
- B01J35/30—Catalysts, in general, characterised by their form or physical properties characterised by their physical properties
- B01J35/39—Photocatalytic properties
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J37/00—Processes, in general, for preparing catalysts; Processes, in general, for activation of catalysts
- B01J37/36—Biochemical methods
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Catalysts (AREA)
Abstract
The invention discloses a method for preparing a zinc oxide photocatalyst with peanut meal protein as a biological template and belongs to the field of photocatalysts. The method includes the steps that peanut meal protein is prepared into a culture medium, flammulina sp is cultured, hyphae suspension liquid and peanut meal protein are obtained and fermented, and the biological template is obtained; the nanometer zinc oxide photocatalyst is prepared through the biological template. According to the method, after the peanut meal protein biological template is subjected to high-temperature calcination, parts of the group is removed, a pore structure is left, the specific area of the prepared nanometer zinc oxide photocatalyst can be increased, excellent photocatalytic performance is displayed, through the honeycomb structure with the peanut meal protein as the template, zinc oxide particles can be good in dispersibility, the adsorption capacity of the zinc oxide catalyst is effectively improved, photocatalytic activity is achieved, the zinc oxide photocatalyst can be separated from a product easily, and it is convenient to use the photocatalyst repeatedly.
Description
Technical field
The invention discloses a kind of Semen arachidis hypogaeae dregs albumen is the method that biological template prepares photocatalyst of zinc oxide, belongs to photocatalyst field.
Background technology
Nano zine oxide (ZnO) is as a kind of new and effective conductor photocatalysis functional material, significantly high quantum yield is shown in organic pollutant degradation, the toxic organic pollutant of some bio-refractory effectively can be degraded at normal temperatures and pressures even mineralising, and its with low cost, product non-secondary pollution, show fabulous application prospect in photocatalysis field.Nano-ZnO is as the photocatalyst of a kind of excellent performance, and its energy gap is close with nano TiO 2, and preparation manipulation is simple, cheap, increasingly draws attention in photocatalysis field.Photocatalyst applications has suspended state and support type two ways.Although suspended state system photocatalytic method has the advantages such as reaction rate height, reactor design is relatively easy, technological operation is convenient, but because Nano semiconductor particle diameter is little, solid-liquid separation reclaims difficulty, causes processing cost to raise, limited in actual applications.Support type photocatalytic method is due to immobilized by Nano semiconductor, it is possible to resolve a difficult problem for nano-powder separation and recovery.
Summary of the invention
nullThe technical problem that present invention mainly solves: the quasiconductor particle diameter for nano zinc oxide photocatalyst is little，Solid-liquid separation reclaims difficulty，Cause the problem that processing cost raises，Providing a kind of Semen arachidis hypogaeae dregs albumen is the method that biological template prepares photocatalyst of zinc oxide，Semen arachidis hypogaeae dregs albumen is prepared into culture medium by the present invention，And Sang Jinqian bacterium is cultivated，Obtain hyphal suspension and Semen arachidis hypogaeae dregs protein fermentation，Obtain biological template，Nano zinc oxide photocatalyst is prepared by biological template，Semen arachidis hypogaeae dregs protein biology template is removed by the present invention through high-temperature calcination rear section group，Leaving pore gap structure，The nano zinc oxide photocatalyst specific surface area prepared is made to increase，Show the photocatalysis performance of excellence，And utilize the alveolate texture that Semen arachidis hypogaeae dregs albumen is template，Make Zinc oxide particles scattered，It is effectively improved the absorbability of Zinc oxide catalytic，Photocatalytic activity、It is prone to separate with product，It is easy to reuse.
In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is:
(1) 200～300g Semen arachidis hypogaeae dregs albumen is weighed, put it into and baking oven dries 1～2h at 50～55 DEG C, by dried Semen arachidis hypogaeae dregs albumen through pulverizing, sieve to obtain 60～80 order powder, by solid-to-liquid ratio 2:1, powder and deionized water are mixed, and in mixture, add 10～20g glucose, 1～3g potassium dihydrogen phosphate, 0.5～0.7g magnesium sulfate, after mixing under 0.09～0.10MPa sterilization treatment 18～23min, after sterilizing culture medium；
(2) choose 6～8 strain Mulberry money bacterium and be inoculated in above-mentioned culture medium, cultivation 20～30h is carried out at 25～30 DEG C, cultivate the deionized water adding 200～300mL after terminating, isolated by filtration obtains hyphal suspension, is mixed with Semen arachidis hypogaeae dregs albumen by liquid-solid ratio 1:2 by hyphal suspension, 30～40 DEG C of fermentations after mixing, 1～2 time is stirred every 2～3h, putting in vacuum drying oven by fermented product after fermentation 20～30h, dry 3～5h at 100～120 DEG C, crushed after being dried, sieve to obtain 50～70 order powder；
(3) weigh the above-mentioned powder of 40～60g and put in 500mL beaker, 200～300mL dehydrated alcohol is added in beaker, regulating pH value with concentration 1mol/L hydrochloric acid solution after stirring is 4～5,3～5h it is standing and soak for after adjustment, after immersion, centrifugation obtains precipitate, by dry after precipitate deionized water wash to filtrate to neutrality, obtain pretreatment Semen arachidis hypogaeae dregs protein biology template；
(4) weigh 5～10g zinc nitrate hexahydrate and 3～8g Disodium oxalate. grinds 30～50min respectively in two mortars, both mixing after grinding, the above-mentioned pretreatment Semen arachidis hypogaeae dregs protein biology template prepared of 10～15g is added in mixture, continue grinding 25～35min, mixture after grinding is put into ultrasonic washing instrument, add the deionized water of 120～150mL, under 120～150W, after ultrasonic cleaning 30～40min, centrifugation obtains precipitate, precipitate deionized water is cleaned 2～3 times, clean post-drying, the dry solid that obtains is put in Muffle furnace high-temperature calcination 50～60min at 500～700 DEG C, 1～2h it is incubated after calcining, room temperature it is cooled to after insulation, photocatalyst of zinc oxide can be obtained.
The application process of the present invention: photocatalyst of zinc oxide degradable methylene blue in waste that the present invention prepares and rhodamine, in the waste water that 8～10L concentration is 60～80mg/L methylene blue and 70～90mg/L rhodamine B, the photocatalyst that the present invention prepares is added by 1～2g/L, by the light source irradiation that wavelength is within the scope of 400～850nm, stirring reaction 30～100min, filtration catalizer, detection degradation rate: the degradation rate of methylene blue is more than 99.5%, and the degradation rate of rhodamine B is more than 99.6%.
The invention has the beneficial effects as follows:
(1) Semen arachidis hypogaeae dregs protein biology template green non-pollution prepared by the present invention, recyclable recycle, stable performance, there is good economic benefit and social benefit in preparing catalyst；
(2) preparation technology of the present invention is simple, easy to operate, preparation condition is gentle and course of reaction is easily controlled, production cost is low；
(3) present invention forms alveolate texture with Semen arachidis hypogaeae dregs albumen for template, and Zinc oxide particles can be made scattered, is effectively improved the absorbability of Zinc oxide catalytic, photocatalytic activity, is prone to separate with product, it is simple to reuses.
Detailed description of the invention
First 200～300g Semen arachidis hypogaeae dregs albumen is weighed, put it into and baking oven dries 1～2h at 50～55 DEG C, by dried Semen arachidis hypogaeae dregs albumen through pulverizing, sieve to obtain 60～80 order powder, by solid-to-liquid ratio 2:1, powder and deionized water are mixed, and in mixture, add 10～20g glucose, 1～3g potassium dihydrogen phosphate, 0.5～0.7g magnesium sulfate, after mixing under 0.09～0.10MPa sterilization treatment 18～23min, after sterilizing culture medium；Choose 6～8 strain Mulberry money bacterium and be inoculated in above-mentioned culture medium, cultivation 20～30h is carried out at 25～30 DEG C, cultivate the deionized water adding 200～300mL after terminating, separate to obtain hyphal suspension, hyphal suspension is mixed with Semen arachidis hypogaeae dregs albumen by liquid-solid ratio 1:2,30～40 DEG C of fermentations after mixing again, 1～2 time is stirred every 2～3h, putting in vacuum drying oven by fermented product after fermentation 20～30h, dry 3～5h at 100～120 DEG C, crushed after being dried, sieve to obtain 50～70 order powder；Weigh the above-mentioned powder of 40～60g and put in 500mL beaker, 200～300mL dehydrated alcohol is added in beaker, regulating pH value with concentration 1mol/L hydrochloric acid solution after stirring is 4～5,3～5h it is standing and soak for after adjustment, after immersion, centrifugation obtains precipitate, by precipitate deionized water wash to filtrate to neutral, dry, obtain pretreatment Semen arachidis hypogaeae dregs protein biology template；Weigh 5～10g zinc nitrate hexahydrate and 3～8g Disodium oxalate. grinds 30～50min respectively in two mortars, both mixing after grinding, the above-mentioned pretreatment Semen arachidis hypogaeae dregs protein biology template prepared of 10～15g is added in mixture, continue grinding 25～35min, mixture after grinding is put into ultrasonic washing instrument, add the deionized water of 120～150mL, under 120～150W, after ultrasonic cleaning 30～40min, centrifugation obtains precipitate, precipitate deionized water is cleaned 2～3 times, clean post-drying, the dry solid that obtains is put in Muffle furnace high-temperature calcination 50～60min at 500～700 DEG C, 1～2h it is incubated after calcining, room temperature it is cooled to after insulation, photocatalyst of zinc oxide can be obtained.
Example 1
First 200g Semen arachidis hypogaeae dregs albumen is weighed, put it into and baking oven dries 1h at 50 DEG C, by dried Semen arachidis hypogaeae dregs albumen through pulverizing, sieve to obtain 60 order powder, by solid-to-liquid ratio 2:1, powder and deionized water are mixed, and in mixture, add 10g glucose, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate, after mixing under 0.09MPa sterilization treatment 18min, after sterilizing culture medium；Choose 6 strain Mulberry money bacterium and be inoculated in above-mentioned culture medium, carry out cultivating 20h at 25 DEG C, cultivate the deionized water adding 200mL after terminating, separate to obtain hyphal suspension, hyphal suspension is mixed with Semen arachidis hypogaeae dregs albumen by liquid-solid ratio 1:2,30 DEG C of fermentations after mixing again, 1 time is stirred every 2h, putting in vacuum drying oven by fermented product after fermentation 20h, dry 3h at 100 DEG C, crushed after being dried, sieve to obtain 50 order powder；Weigh the above-mentioned powder of 40g and put in 500mL beaker, 200mL dehydrated alcohol is added in beaker, regulating pH value with concentration 1mol/L hydrochloric acid solution after stirring is 4,3h it is standing and soak for after adjustment, after immersion, centrifugation obtains precipitate, by precipitate deionized water wash to filtrate to neutral, dry, obtain pretreatment Semen arachidis hypogaeae dregs protein biology template；The zinc nitrate hexahydrate and the 3g Disodium oxalate. that weigh 5g grind 30min respectively in two mortars, both mixing after grinding, the above-mentioned pretreatment Semen arachidis hypogaeae dregs protein biology template prepared of 10g is added in mixture, continue to grind 25min, mixture after grinding is put into ultrasonic washing instrument, add the deionized water of 120mL, under 120W, after ultrasonic cleaning 30～40min, centrifugation obtains precipitate, precipitate deionized water is cleaned 2 times, clean post-drying, the dry solid that obtains is put in Muffle furnace high-temperature calcination 50min at 500 DEG C, 1h it is incubated after calcining, room temperature it is cooled to after insulation, photocatalyst of zinc oxide can be obtained.
Photocatalyst of zinc oxide degradable methylene blue in waste that the present invention prepares and rhodamine, in the waste water that 8L concentration is 60mg/L methylene blue and 70mg/L rhodamine B, the photocatalyst that the present invention prepares is added by 1g/L, by the light source irradiation that wavelength is within the scope of 600nm, stirring reaction 30min, filtration catalizer, detects degradation rate: the degradation rate of methylene blue is 99.6%, and the degradation rate of rhodamine B is 99.7%.
Example 2
First 250g Semen arachidis hypogaeae dregs albumen is weighed, put it into and baking oven dries 1.5h at 53 DEG C, by dried Semen arachidis hypogaeae dregs albumen through pulverizing, sieve to obtain 70 order powder, by solid-to-liquid ratio 2:1, powder and deionized water are mixed, 15g glucose, 2g potassium dihydrogen phosphate, 0.6g magnesium sulfate is added in mixture, after mixing under 0.095MPa sterilization treatment 20min, after sterilizing culture medium；Choose 6～8 strain Mulberry money bacterium and be inoculated in above-mentioned culture medium, and carry out cultivating 25h at 27 DEG C, cultivate the deionized water adding 250mL after terminating, separate to obtain hyphal suspension, hyphal suspension is mixed with Semen arachidis hypogaeae dregs albumen by liquid-solid ratio 1:2,35 DEG C of fermentations after mixing again, 1 time is stirred every 2.5h, putting in vacuum drying oven by fermented product after fermentation 25h, dry 4h at 110 DEG C, crushed after being dried, sieve to obtain 60 order powder；Weigh the above-mentioned powder of 50g and put in 500mL beaker, 250mL dehydrated alcohol is added in beaker, regulating pH value with concentration 1mol/L hydrochloric acid solution after stirring is 4.5,4h it is standing and soak for after adjustment, after immersion, centrifugation obtains precipitate, by precipitate deionized water wash to filtrate to neutral, dry, obtain pretreatment Semen arachidis hypogaeae dregs protein biology template；The zinc nitrate hexahydrate and the 5g Disodium oxalate. that weigh 8g grind 40min respectively in two mortars, both mixing after grinding, the above-mentioned pretreatment Semen arachidis hypogaeae dregs protein biology template prepared of 13g is added in mixture, continue to grind 30min, mixture after grinding is put into ultrasonic washing instrument, add the deionized water of 135mL, under 135W, after ultrasonic cleaning 35min, centrifugation obtains precipitate, precipitate deionized water is cleaned 2 times, clean post-drying, the dry solid that obtains is put in Muffle furnace high-temperature calcination 55min at 600 DEG C, 1.5h it is incubated after calcining, room temperature it is cooled to after insulation, photocatalyst of zinc oxide can be obtained.
The application process of the present invention: photocatalyst of zinc oxide degradable methylene blue in waste that the present invention prepares and rhodamine, in the waste water that 9L concentration is 70mg/L methylene blue and 80mg/L rhodamine B, the photocatalyst that the present invention prepares is added by 1.5g/L, by the light source irradiation that wavelength is within the scope of 750nm, stirring reaction 80min, filtration catalizer, detects degradation rate: the degradation rate of methylene blue is 99.7%, and the degradation rate of rhodamine B is 99.8%.
Example 3
First 300g Semen arachidis hypogaeae dregs albumen is weighed, put it into and baking oven dries 2h at 55 DEG C, by dried Semen arachidis hypogaeae dregs albumen through pulverizing, sieve to obtain 80 order powder, by solid-to-liquid ratio 2:1, powder and deionized water are mixed, 20g glucose, 3g potassium dihydrogen phosphate, 0.7g magnesium sulfate is added in mixture, after mixing under 0.10MPa sterilization treatment 23min, after sterilizing culture medium；Choose 8 strain Mulberry money bacterium and be inoculated in above-mentioned culture medium, and carry out cultivating 30h at 30 DEG C, cultivate the deionized water adding 300mL after terminating, separate to obtain hyphal suspension, hyphal suspension is mixed with Semen arachidis hypogaeae dregs albumen by liquid-solid ratio 1:2,40 DEG C of fermentations after mixing again, 2 times are stirred every 3h, putting in vacuum drying oven by fermented product after fermentation 30h, dry 5h at 120 DEG C, crushed after being dried, sieve to obtain 70 order powder；Weigh the above-mentioned powder of 60g and put in 500mL beaker, 300mL dehydrated alcohol is added in beaker, regulating pH value with concentration 1mol/L hydrochloric acid solution after stirring is 5,5h it is standing and soak for after adjustment, after immersion, centrifugation obtains precipitate, by precipitate deionized water wash to filtrate to neutral, dry, obtain pretreatment Semen arachidis hypogaeae dregs protein biology template；The zinc nitrate hexahydrate and the 8g Disodium oxalate. that weigh 10g grind 50min respectively in two mortars, both mixing after grinding, the above-mentioned pretreatment Semen arachidis hypogaeae dregs protein biology template prepared of 15g is added in mixture, continue to grind 35min, mixture after grinding is put into ultrasonic washing instrument, add the deionized water of 150mL, under 150W, after ultrasonic cleaning 40min, centrifugation obtains precipitate, precipitate deionized water is cleaned 3 times, clean post-drying, obtain solid and put in Muffle furnace high-temperature calcination 60min at 700 DEG C, 2h it is incubated after calcining, room temperature it is cooled to after insulation, photocatalyst of zinc oxide can be obtained.
The application process of the present invention: photocatalyst of zinc oxide degradable methylene blue in waste that the present invention prepares and rhodamine, in the waste water that 10L concentration is 80mg/L methylene blue and 90mg/L rhodamine B, the photocatalyst that the present invention prepares is added by 2g/L, by the light source irradiation that wavelength is within the scope of 850nm, stirring reaction 100min, filtration catalizer, detects degradation rate: the degradation rate of methylene blue is 99.8%, and the degradation rate of rhodamine B is 99.9%.
Claims (1)
1. a Semen arachidis hypogaeae dregs albumen is the method that biological template prepares photocatalyst of zinc oxide, it is characterised in that concrete preparation process is:
(1) 200～300g Semen arachidis hypogaeae dregs albumen is weighed, put it into and baking oven dries 1～2h at 50～55 DEG C, by dried Semen arachidis hypogaeae dregs albumen through pulverizing, sieve to obtain 60～80 order powder, by solid-to-liquid ratio 2:1, powder and deionized water are mixed, and in mixture, add 10～20g glucose, 1～3g potassium dihydrogen phosphate, 0.5～0.7g magnesium sulfate, after mixing under 0.09～0.10MPa sterilization treatment 18～23min, after sterilizing culture medium；
(2) choose 6～8 strain Mulberry money bacterium and be inoculated in above-mentioned culture medium, cultivation 20～30h is carried out at 25～30 DEG C, cultivate the deionized water adding 200～300mL after terminating, isolated by filtration obtains hyphal suspension, is mixed with Semen arachidis hypogaeae dregs albumen by liquid-solid ratio 1:2 by hyphal suspension, 30～40 DEG C of fermentations after mixing, 1～2 time is stirred every 2～3h, putting in vacuum drying oven by fermented product after fermentation 20～30h, dry 3～5h at 100～120 DEG C, crushed after being dried, sieve to obtain 50～70 order powder；
(3) weigh the above-mentioned powder of 40～60g and put in 500mL beaker, 200～300mL dehydrated alcohol is added in beaker, regulating pH value with concentration 1mol/L hydrochloric acid solution after stirring is 4～5,3～5h it is standing and soak for after adjustment, after immersion, centrifugation obtains precipitate, by dry after precipitate deionized water wash to filtrate to neutrality, obtain pretreatment Semen arachidis hypogaeae dregs protein biology template；
(4) weigh 5～10g zinc nitrate hexahydrate and 3～8g Disodium oxalate. grinds 30～50min respectively in two mortars, both mixing after grinding, the above-mentioned pretreatment Semen arachidis hypogaeae dregs protein biology template prepared of 10～15g is added in mixture, continue grinding 25～35min, mixture after grinding is put into ultrasonic washing instrument, add the deionized water of 120～150mL, under 120～150W, after ultrasonic cleaning 30～40min, centrifugation obtains precipitate, precipitate deionized water is cleaned 2～3 times, clean post-drying, the dry solid that obtains is put in Muffle furnace high-temperature calcination 50～60min at 500～700 DEG C, 1～2h it is incubated after calcining, room temperature it is cooled to after insulation, photocatalyst of zinc oxide can be obtained.
Priority Applications (1)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107199030A (en) * | 2017-06-30 | 2017-09-26 | 常州豫春化工有限公司 | A kind of preparation method of cellular porous bismuth oxide |
| CN108059184A (en) * | 2017-12-28 | 2018-05-22 | 兰州大学 | A kind of method that ZnO nanoparticle is prepared using recombined collagen as biomineralization template |
| CN108821328A (en) * | 2018-09-28 | 2018-11-16 | 胡果青 | A kind of preparation method of nano zine oxide |
-
2016
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107199030A (en) * | 2017-06-30 | 2017-09-26 | 常州豫春化工有限公司 | A kind of preparation method of cellular porous bismuth oxide |
| CN108059184A (en) * | 2017-12-28 | 2018-05-22 | 兰州大学 | A kind of method that ZnO nanoparticle is prepared using recombined collagen as biomineralization template |
| CN108059184B (en) * | 2017-12-28 | 2020-10-27 | 兰州大学 | Method for preparing ZnO nanoparticles by taking recombinant collagen as biomineralization template |
| CN108821328A (en) * | 2018-09-28 | 2018-11-16 | 胡果青 | A kind of preparation method of nano zine oxide |
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