CN105794954A - Method of bamboo leaf flavonoid combined with pichia caribbica to prevent and treat apple postharvest diseases - Google Patents
Method of bamboo leaf flavonoid combined with pichia caribbica to prevent and treat apple postharvest diseases Download PDFInfo
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- CN105794954A CN105794954A CN201610143541.9A CN201610143541A CN105794954A CN 105794954 A CN105794954 A CN 105794954A CN 201610143541 A CN201610143541 A CN 201610143541A CN 105794954 A CN105794954 A CN 105794954A
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- folium bambosae
- bambosae flavone
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
- A23B7/155—Microorganisms; Enzymes; Antibiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention discloses a method of bamboo leaf flavonoid combined with pichia caribbica to prevent and treat apple postharvest diseases, and belongs to the biological prevention and treatment field of fruit postharvest diseases. The pichia caribbica is activated, the activated pichia caribbica is inoculated into a NYDB culture medium to be cultured for 20 h, the cultured pichia caribbica is centrifuged to obtain the pichia caribbica cells, the pichia caribbica cells are diluted into pichia caribbica suspension at 1x10^8 cells/mL using the bamboo leaf flavonoid at twice final concentration (the final concentration of the bamboo leaf flavonoid is 100 [mu]g/mL-500 [mu]g/mL); the pichia caribbica suspension is uniformly sprayed on apples and the apples are dried naturally; the dried apples are placed in plastic baskets, the appless are sealed with preservation films, and the apples are placed in a room temperature (20 DEG C) to be stored. The combination of the bamboo leaf flavonoid and the pichia caribbica improves the effectiveness of the biological prevention and treatment of the apple post-harvest diseases. The method is easy to use, convenient to operate, good in effects and low in costs.
Description
Technical field
The present invention relates to a kind of Folium Bambosae flavone (BLF) combine Cali's Bick Pichia sp. (Pichia caribbica) preventing and treating Fructus Mali pumilae and adopt
The method of rear disease, belongs to biologically controlling postharvest disease of fruit field.
Background technology
Fructus Mali pumilae is one of big fruit in the world four, is the most important fruit tree species of Temperate Region in China, world cultivation, in world's fruit market
In occupy an important position.The nutritional labeling of Fructus Mali pumilae is the abundantest.Containing protein, fat, carbohydrate, vitamin C,
Organic acid and pectin, the fibre such as vitamin B1, vitamin B2, carotene and calcium, phosphorus, ferrum, citric acid, tartaric acid
Dimension element etc..At present, whole world apple production state (regional) has about 80, and China and South America are Apple developments over nearly 20 years
The fastest countries and regions.Calendar year 2001 world's Fructus Mali pumilae area 568.5 ten thousand hectares, yield 6.3 × 1010Kilogram, wherein China's Fructus Mali pumilae
Area 240.1 ten thousand hectares, yield 2.4 × 1010Kilograms ten thousand tons, account for world's Fructus Mali pumilae gross area and the 42.2% of total output and 38.2%,
Occupy first place in the world in all positions.
The loss that Biocontrol of Postharvest Diseases in Apple Fruits causes is huge.According to general conservative estimation, after China's Fructus Mali pumilae is adopted, loss is 10%
About-30%.After causing Fructus Mali pumilae to be adopted loss and deterioration because have a lot, including the mistake caused due to moisture and nutrient consumption
Weight and the quality fission such as withered；Owing to adopting the physiological metabolism imbalance that rear adverse circumstance causes；Owing to adopting the fruit group that after ripening aging causes
Knit structure collapse and due to pathogen infect cause rot etc..Wherein due to pathogen infection cause rotten be to cause fruit
The main cause of loss after adopting.Cause the fungal species rotted during fruit storage a lot, but every kind of dominant pathogenic bacterium of fruit
Typically there is 2-3 kind.Such as, it is mainly penicillium expansum (Penicillium expansum) by fungus-caused disease after Fructus Mali pumilae is adopted
The penicilliosis caused and the gray mold caused by Botrytis cinerea (Botrytis cinerea).Pathogenic fungi is to adopting the harm of rear fruit not
It is only that it causes fruit heavy losses quantitatively, and many pathogenic fungi can secrete the many secondary metabolite meetings of generation
Causing serious food-safety problem, the Main Pathogenic Bacteria penicillium expansum (P.expansum) on Fructus Mali pumilae can produce clavacin
(patulin).Patulin, also known as clavacin, is that the one that the mycete metabolism such as Penicillium, aspergillus, the mould genus of silk clothes produce has
The fungus metabolite of poison, has the harmful effects such as carcinogenic, aberration inducing is the most lethal.
For a long time, the major measure controlling postharvest disease of fruits and vegetables is to use antibacterial.Adding up according to latest data, antibacterial exists
Crop disease control market accounts for 26% share, and within 2011, consumption sum is up to 13,300,000,000 dollars.Chemical bactericide have economy,
Bactericidal effect is good, the feature of instant effect, the conventional chemical bactericide controlling fruit postharvest diseases mainly have imazalil (Imazzlii),
Benomyl (Benomyl), Guanoctine (Guazatine).But more than ten years in past, the public and scientific circles are in food and environment
The concern of pesticide (especially antibacterial) residual hazard problem increases day by day.Long-term and a large amount of use of antibacterial, serious environment pollution,
It is harmful to human health.The report (1987) of NAS (NAS) is pointed out, the application of antibacterial is to human health
Cause harm；In the sales volume of the 9 kinds of neoplastic compounds in the whole America, antibacterial accounts for about 90%, at the whole pesticide for food
In, the tumorigenesis that antibacterial constitutes 60% is dangerous.Resist additionally, altofrequency ground uses the antibacterial of single kind that pathogen can be made to produce
Property of medicine problem, makes the prevention effect of antibacterial be substantially reduced.
Therefore, study and utilize the substitute of chemical bactericide to prevent and treat the disease of plant especially fruit and vegerable, being of facing mankind
Urgent task.Biofungicide can alleviate harmful microorganism and infect fruit and vegerable, can protect again environment, is production security food
Effective ways.Thus research natural biological antibacterial replaces pesticide and carries out the preventing and treating of postharvest disease of fruits and vegetables, to reducing food middle peasant
The residual of medicine, protects environment, it is ensured that health, increases fruit and vegerable outlet, all has deep meaning.
Current the most increasingly attention beneficial microbe carries out the research of biologically controlling postharvest disease of fruit, and it is thought that
It is hopeful one of direction of substituted chemistry antibacterial.Yeast is due to inheritance stability, and antimicrobial spectrum is wide, titer is high, does not produces
The metabolite harmful to people and host plant, safety is high and low to nutritional requirement, and growth is fast, and to various abiotic stress, inverse
Border has stronger tolerance, insensitive to most of antibacterial, and chemically and physically processing other can be compatible, the most in recent years
Carry out antagonism yeast as fruit postharvest diseases biocontrol microorganisms, by international extensive concern.
Since nineteen fifty-three, since the reported first such as Gutter bacillus subtilis has antagonism to citrus fruit pathogen, profit
With Antagonistic Fungi carry out the Biological control of fruit postharvest diseases at present achieved with breakthrough progress it is considered to be most be hopeful to substitute
The method of chemical bactericide.Such as in the U.S., first Biological control microorganism is used to make from Environmental Protection Agency approval in 1979
Since biocontrol microorganisms, by the end of 2004, existing 26 microorganisms (14 kinds of antibacterials and 12 kinds of funguses) were approved also by this mechanism
Registration.Pichia sp. (Pichia guilliermondii) is the Biological control being in the news the most the earliest and suppressing fruit pathogenic fungi
Yeast, and Candida oleophilia isolate I-182 in nineteen ninety-five as biofungicide at Environmental Protection Agency
(EPA) registration, and by U.S.'s Ecogen company trade.Additionally, after South Africa one strain is adopted antagonism yeast Bai Yinqiu ferment
Female (Cryptococcus albidus) the most commercialization, trade name Yield Plus.
Up to the present, the existing semicentennial history of the research of postharvest fruit and vegetable Biological control, but it is applied to the antagonism produced
Strain class is few, a few launch such as only Biosave and Aspire etc., and China does not the most still have Antagonistic Fungi to be applied to
Actual production.Main reason is that Antagonistic Fungi that document reports is mostly for preventing and treating the pathogen rather than directly of fruit and vegerable wound
It is used in fruit and vegerable epidermis and prevents and treats the pathogen invasion of normal fruit and vegerable；On the other hand, under the conditions of commodity production, due to environmental condition
Uncertainty, its preventive effect or do not reach requirement or unstable, and cost accounting is higher compared with chemical bactericide；Antagonistic Fungi
As antistaling agent, its occupation mode is convenient not as good as chemical bactericide.Additionally, antagonism yeast needs could effectively make under higher concentration
Feature also will produce the biggest Financial cost pressure.These factors affect Antagonistic Fungi is as fruit and vegetable fresh-keeping agent aborning
Business use.Therefore the effect of antagonism yeast Biological control must be further improved and improve.
Biocontrol effect is the important indicator judging Antagonistic Fungi good job, is also to judge that can microorganism Antagonistic Fungi be applied to actual life
The foundation produced.The research of recent years shows, can from following several in terms of improve the biocontrol effect of Antagonistic Fungi.1. use short of money
Antibacterial mixture is used in mixed way Antagonistic Fungi three big advantages: (1) can widen sphere of action, prevents and treats multiple pathogen；(2)
Effectiveness can be strengthened, reduce and use frequency；(3) can allow to combine different Biological control feature.2. be combined with chemical bactericide
Biological preparation is used to be effectively combined with chemical bactericide to control disease with the antibacterial more less than conventional antimicrobial agent dose
Evil is possibly realized, thus the usage amount of antibacterial is reduced to the safe dose level below to human body, reduces the pesticide of fruit and vegerable
Residual.3. use adjuvant to use some adjuvant can increase antagonistic microbe colony number and strengthen its survival rate, and then increase short of money
Antibacterial biological and ecological methods to prevent plant disease, pests, and erosion ability.4. it is used in mixed way 5. effects being combined other measures with some metal ion and many studies have shown that Jiang Rechu
The methods such as reason, cold preservation are used in combination with biocontrol bacterium, can strengthen the fungistatic effect of Antagonistic Fungi.
Folium Bambosae flavone is the biological flavone with physiologically active extracted from Folium Bambusae, is a kind of efficient biologicalAntioxidant
(Anti-Oxidant), modem animal toxicological experiment fully demonstrates the security reliability of Folium Bambosae flavone.As Folium Bambusae function extract,
Folium Bambosae flavone, rich in Flavonoid substances, is widely used in food additive.Folium Bambusae contains active skull cap components, has many-side
Biological function effect and extraordinary free radical scavenging effect.It addition, as food additive, research shows that Folium Bambosae flavone is made
It is used in combination for antioxidant and antagonism yeast, activities of antioxidant enzymes and the antagonism yeast toleration to oxidative stress can be improved,
Thus improve its Biological control effect to fruit postharvest diseases.
Cali's Bick Pichia sp. has been proved to have antagonistic potencies, and the present invention utilizes Folium Bambosae flavone and Cali's Bick Pichia sp.
It is used in combination its effect to biologically controlling postharvest disease of fruit of raising, thus reduces the rotting rate of fruit, and then reach fruit storage
Hide fresh-keeping purpose.
Summary of the invention
The present invention utilizes Folium Bambosae flavone (BLF) and Cali's Bick Pichia sp. to be used in combination, and improves after fruit is adopted by this yeast sick
The Biological control effect of evil.Compared with Cali's Bick Pichia sp. individual processing, Folium Bambosae flavone and Cali's Bick Pichia sp. combine
Use, this yeast inhibitory action to green mold of apple can be strengthened significantly, prevent and treat its postharvest decay.The present invention has safety,
Efficiently, low cost and other advantages, during can being widely used for the Biological control of fruit postharvest diseases, reduces fruit postharvest diseases
The loss caused.
The technical solution used in the present invention
The invention provides the method that Folium Bambosae flavone combines Cali's Bick Pichia sp. preventing and treating Biocontrol of Postharvest Diseases in Apple Fruits, as steps described below
Carry out: Cali's Bick Pichia sp. (Pichia caribbica) is activated, finishes red ferment with NYDB culture medium culturing Cali Bick
Mother, the centrifugal thalline that obtains, preparation contains the 1 × 10 of Folium Bambosae flavone8The yeast suspension of cells/mL；Bacteria suspension is uniformly sprayed at
On fruit, natural air drying；It is placed in plastic crate, after sealing with preservative film, deposits under the conditions of being placed on room temperature (20 DEG C).
Wherein said NYDB culture medium is: beef extract 8g, yeast extract 5g, glucose 10g, distilled water 1000ml,
PH is natural.
Wherein said preparation contains the 1 × 10 of Folium Bambosae flavone8Cells/mL yeast-like fungi suspension method, is carried out: as steps described below by bacterium
The dilution of body sterilized water is prepared as 2 × 108Cells/mL bacteria suspension, with the Folium Bambosae flavone that concentration is twice final concentration 1:1 by volume
Mixing, and the ultimate density of Folium Bambosae flavone is 100 μ g/mL-500 μ g/mL.
Advantages of the present invention:
(1) Cali used in the present invention Bick Pichia sp. (Pichia caribbica) is this laboratory screening, and its antagonism is imitated
Power is strong, harmless.
(2) Folium Bambosae flavone used in the present invention (BLF) wide material sources, cheap, nontoxic.
(3) present invention uses Folium Bambosae flavone and Cali's Bick Pichia sp. to be used in combination, and processes or card with being used alone Folium Bambosae flavone
Libiee gram Pichia sp. processes and compares, and can significantly improve the antagonistic activity of Cali's Bick Pichia sp., thus reduce the corruption of fruit
Rotten rate, and then reach the purpose that fruit storage is fresh-keeping.
(4) present invention uses Folium Bambosae flavone and Cali's Bick Pichia sp. to be used in combination, and can substitute for chemical bactericide preventing and treating
Fruit postharvest diseases, it is to avoid use the chemical bactericide harm to people, there is significant economic benefit and social benefit.
Accompanying drawing explanation
Fig. 1 is that Folium Bambosae flavone is used in combination the control effect to green mold of apple with Cali's Bick Pichia sp..Note: CK: aseptic
Distilled water, i.e. matched group；Pc:1 × 108The P.carribbica yeast suspension of cells/ml；0.01%BLF:100 μ g/mL
Folium Bambosae flavone (BLF)；The Folium Bambosae flavone (BLF) of 0.025%BLF:250 μ g/mL；0.05%BLF:500 μ g/mL
Folium Bambosae flavone (BLF)；0.01%BLF+Pc:1 × 108The P.carribbica yeast suspension of cells/ml is (containing final concentration
It is the BLF of 100 μ g/mL)；0.025%BLF+Pc:1 × 108The P.carribbica yeast suspension of cells/ml is (containing the denseest
Degree is the BLF of 250 μ g/mL)；0.05%BLF+Pc:1 × 108The P.carribbica yeast suspension of cells/ml is (containing eventually
Concentration is the BLF of 500 μ g/mL).Different letters represent significant difference (P < 0.05).
Fig. 2 is that Folium Bambosae flavone is used in combination the impact naturally rotted Fructus Mali pumilae with Cali's Bick Pichia sp..Note: CK: aseptic steaming
Distilled water, i.e. matched group；The Folium Bambosae flavone of 0.01%BLF:100 μ g/mL；Pc:1 × 108The P.carribbica ferment of cells/ml
Female bacteria suspension；0.01%BLF+Pc:1 × 108The P.carribbica yeast suspension of cells/ml is (containing final concentration of 100 μ g/mL
BLF).Different letters represent significant difference (P < 0.05).
Detailed description of the invention
By the present invention being will be explained in more detail by following embodiment.Following example are merely illustrative, and the present invention is also
Do not limited by these embodiments.
Embodiment 1 Cali Bick Pichia sp. (P.carribbica) is that this laboratory screening obtains, and application Chinese invention patent also authorizes,
Patent No. ZL201010198119.6, patent name Cali Bick Pichia sp., application in fruit storage is fresh-keeping and user
Method.In NYDA (adding the agar (quality meter) of 2% on the basis of NYDB culture medium) 4 DEG C of cryopreservation of culture medium.NYDB
Yeast extract 5g in culture medium, beef extract 8g, glucose 10g, purified water l000ml, natural pH.
Cultivation program is: (1) solid activates: be inoculated in NYDA culture medium by Cali's Bick Pichia sp., 28 DEG C of cultivations
48h；(2) liquid culture: load the NYDB seed culture medium of 50ml in the triangular flask of 250ml, accesses one with inoculating loop
Cali's Bick Pichia sp. that ring activates, at 180rpm, cultivates 20h under the conditions of 28 DEG C；(3) centrifugation settling flux: on
State Yeast Cultivation mixture under the conditions of 8000g, centrifugal 10min, and wash 2 times with sterile distilled water, it is situated between to remove to cultivate
Matter, with sterile distilled water again settling flux, and is 2 × 10 with blood counting chamber regulation barm cell concentration8Cells/ml, then with going out
Distilled water or Folium Bambosae flavone (BLF) solution of crossing bacterium are diluted to desired concn.
Embodiment 2 Folium Bambosae flavone is used in combination the inhibition to Fructus Mali pumilae Postharvest Penicillium with Cali's Bick Pichia sp.
One) testing program
Supplying the fruit of examination through fine selection, select fruit uniformity as far as possible, surface is without scratch！Mechanical injury and disease pest injury.By Herba Marsileae Quadrifoliae
Fruit is punched with aseptic card punch, and superficial cut is 5mm (diameter) × 3mm (deeply).Each wound equivalent adds 30 below μ l and processes
Liquid: (1) 1 × 108Cali's Bick Pichia sp. suspension (containing each concentration Folium Bambosae flavone) of cells/mL；(2)1×108The card of cells/mL
Libiee gram Pichia sp. bacteria suspension；(3) sterilized water.After 3h, then inoculate 30 below μ L pathogenic bacterium spores respectively to fruit wound outstanding
Supernatant liquid: inoculate 5 × 10 to Fructus Mali pumilae wound4Penicillium expansum (Penicillium expanusm) spore suspension of spores/mL.
Naturally, after drying, fruit put into plastic crate and seals with preservative film, constant incubator cultivating (humidity 95%), is placed in 20 DEG C.Often
Process is repeated 3 times, and repeats 12 fruits every time.Whole test is repeated 2 times.After 8 days, record fruit sickness rate, comment with this
The fungistatic effect of valency Cali Bick Pichia sp..
The computing formula of sickness rate is as follows:
The fruit of sickness rate (%)=morbidity/fruit sum × 100%
Two) result of the test
Testing according to above-mentioned steps, the rotting rate result of statistics fruit is as follows:
As shown in fig. 1, the sickness rate of matched group is about 91.7%, and single yeast process group sickness rate is 22.2%, therefore
P.caribbica can effectively control to be caused by penicillium expansum the postharvest disease of Fructus Mali pumilae at 20 DEG C.Meanwhile, with Cali's Bick
Pichia sp. individual processing group is compared, and Folium Bambosae flavone can significantly increase the biocontrol effect of yeast.Wherein 100ug/ml BLF knot
Closing Cali's Bick Pichia sp. process group and have best antiseptic effect, its green mold of apple disease sickness rate is only 5.6%, i.e. effect
The most notable.
Embodiment 3 Folium Bambosae flavone is used in combination with Cali's Bick Pichia sp. and naturally rots Fructus Mali pumilae and the impact of storage quality
One) testing program
Following treatment fluid is uniformly sprayed on fruit surface, naturally dries: (1) 1 × 108Cali's Bick Pichia yeast of cells/mL
Suspension (BLF concentration is 100 μ g/mL)；(2)1×108Cali's Bick Pichia sp. bacteria suspension of cells/mL；(3) BLF is (dense
Degree is 100 μ g/mL) and (4) sterilized water.Then, the fruit handled well is put into plastic crate preservative film to seal.20 DEG C (RH95%)
Constant incubator in preserve 35 days.Started to take out every 5 days fruit statistics rot fruit number from the 10th day, and observe rotten
Situation.After 35 days, survey fruit storage quality.Often processing and be repeated 3 times, whole experiment is repeated 2 times.Folium Bambosae flavone and card Libiee
Gram Pichia sp. is used in combination the inhibition naturally rotted fruit and represents with sickness rate.
1, the computing formula of sickness rate is as follows:
The fruit of sickness rate (%)=morbidity/fruit sum × 100%
2, quality determination method is as follows:
(1) total dissoluble solid content: measure with hand-held saccharometer, uses saccharometer to be measured, measurement result
It is expressed as g/100g sample.
(2) brown stain degree: weigh 20g sarcocarp from 6 fruits, adds 40ml distilled water and grinds rapidly, 10000 × g, 4 DEG C of bars
Centrifugal 10mim under part, takes centrifugal 10mim under the conditions of supernatant 10ml adds 95% ethanol 15ml, 10000 × g, 4 DEG C, takes
Supernatant measures absorbance in 420nm, represents browning degree with this.Often process 3 times and repeat.
(3) titrable acidity: use titrimetry, from 5 fruit samplings, weighs 10g sample, adds distilled water and grind rapidly,
It is settled to 100mL, filters, take 10mL filtrate, carry out being titrated to pH 8.1 with 0.1mol/L NaOH, carry out after titration
Calculating, result represents with the percent containing malic acid.
(4) Vc content: 2,6-Dichlorophenol indophenol methods.From 5 fruit, take 5g sample add the grinding homogenate of 5mL 2% oxalic acid,
Pour 100mL volumetric flask into, with 1% oxalic acid washing residue, add 1mL 30% zinc sulfate, shake up.Add the ferrous cyanogen of 1mL 15% again
Change potassium (depigmentation), with 1% oxalic acid constant volume, shake up filtration.Take filtrate 4mL, then add 2 to each pipe, 6-Dichlorophenol
Indophenol dye solution, adds dimethylbenzene 5mL immediately, shakes rapidly about 0.5min, and after standing, dimethylbenzene separates with water layer, will
Upper strata xylene extraction liquid measures absorbance under 500nm wavelength.Optical density value according to measuring liquid looks into standard curve, obtains phase
The content of the vitamin C (Vc) answered, result milligram Vc/100g fruit represents.
Two) result of the test
1, testing according to above-mentioned steps, the natural rotting rate result of statistics is as follows:
Figure it is seen that after 35 days, BLF individual processing group, Cali's Bick Pichia sp. individual processing group, Cali's Bick
The Apple sickness rate that Pichia sp. and BLF combination process all is substantially less than matched group (79.4%).Folium Bambosae flavone and card Libiee
Gram Pichia sp. combination process group fruit rot rate is minimum, and only 8.3%, this is substantially less than used alone BLF and Cali's Bick
The natural rotting rate of Pichia sp..Illustrate that BLF (concentration is 100 μ g/mL) can significantly improve Cali's Bick Pichia sp. pair
The prevention effect of Biocontrol of Postharvest Diseases in Apple Fruits.
2, testing according to above-mentioned steps, the storage quality result of statistics is as follows:
Table 1 Folium Bambosae flavone is used in combination the impact on Apples quality with Cali's Bick Pichia sp.
Note: CK: sterile distilled water, i.e. matched group；The Folium Bambosae flavone of 0.01%BLF:100 μ g/mL；Pc:1 × 108The P.carribbica of cells/ml
Yeast suspension；0.01%BLF+Pc:1 × 108The P.carribbica yeast suspension of cells/ml.Different letters represent significant difference (P < 0.05).
As it can be seen from table 1 after 35 days, compared with matched group, be combined the Fructus Mali pumilae processed with Cali's Bick Pichia sp. through BLF
Its Vc content, titratable acid and brown stain degree significantly reduce without significant difference, soluble solid, do not produce every index of quality
Raw adverse effect.
Claims (4)
1. the method that Folium Bambosae flavone combines Cali Bick Pichia sp. preventing and treating Biocontrol of Postharvest Diseases in Apple Fruits, is carried out as steps described below: by Cali's Bick Pichia sp. (Pichia caribbica) activation, with NYDB culture medium culturing Cali Bick Pichia sp., the centrifugal thalline that obtains, preparation contains the 1 × 10 of Folium Bambosae flavone8The yeast suspension of cells/mL；Bacteria suspension is uniformly sprayed on fruit, natural air drying；It is placed in plastic crate, after sealing with preservative film, deposits under the conditions of being placed on room temperature (20 DEG C).
Folium Bambosae flavone the most according to claim 1 combines the method that Cali's Bick Pichia sp. uses preventing and treating fruit postharvest diseases, it is characterized in that wherein said NYDB culture medium is: beef extract 8 g, yeast extract 5 g, glucose 10 g, distilled water 1000 ml, pH are natural.
Folium Bambosae flavone the most according to claim 1 combines the method that Cali's Bick Pichia sp. uses preventing and treating fruit postharvest diseases, it is characterised in that wherein said preparation contains the 1 × 10 of Folium Bambosae flavone8 Cells/mL yeast suspension method, is carried out as steps described below: the dilution of thalline sterile distilled water is prepared as 2 × 108 Cells/mL bacteria suspension, mixes with the Folium Bambosae flavone that concentration is twice final concentration 1:1 by volume, and the ultimate density of Folium Bambosae flavone is 100 μ g/mL.
Folium Bambosae flavone the most according to claim 1 combines the method that Cali's Bick Pichia sp. uses preventing and treating fruit postharvest diseases, it is characterised in that wherein said fruit is Fructus Mali pumilae.
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