CN105794860A - Microbial agent for controlling quarantine fungus diseases of apples and promoting yield increase - Google Patents
Microbial agent for controlling quarantine fungus diseases of apples and promoting yield increase Download PDFInfo
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- CN105794860A CN105794860A CN201610211999.3A CN201610211999A CN105794860A CN 105794860 A CN105794860 A CN 105794860A CN 201610211999 A CN201610211999 A CN 201610211999A CN 105794860 A CN105794860 A CN 105794860A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Abstract
The invention discloses a microbial agent for preventing and controlling quarantine fungus diseases of apples and promoting yield increase. The microbial agent is prepared from raw materials in parts by weight as follows: 10-15 parts of streptomyces microflavus, 10-15 parts of bacillus subtilis, 15-25 parts of chaetomium globosum, 8-10 parts of bacillus laterosporus, 15-20 parts of bacillus amyloliquefaciens, 8-10 parts of bacillus circulans, 10-20 parts of trichoderma harzianum, 5-10 parts of trichoderma asperellum, 5-10 parts of trichoderma fasciculatum bissett, 5-10 parts of paecilomyces lilacinus, 8-12 parts of streptomyces syringini, 8-12 parts of gordonia, 8-12 parts of conexibacter, 5-10 parts of alga oligosaccharide and 10-15 parts of chitosan oligosaccharide citrate. The microbial agent can effectively inhibit the quarantine fungus diseases and can strengthen apple trees, and the yield increase purpose can be achieved; chaetomium globosum has remarkable bactericidal and bacteriostatic activity for valsa ceratosperma of the apple trees; no pesticides are used, the microbial agent is non-toxic, harmless, pollution-free and residue-free, the quality and the safety of agricultural products are guaranteed, and the apples have pure taste and high sweetness.
Description
Technical field
The present invention relates to field of plant disease control, a kind of for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase.
Background technology
Fructus Mali pumilae belongs to Rosaceae, deciduous tree, elliptic leaf shape, has sawtooth；Fruit is spherical, and taste is sweet, is common fruit, is also modal fruit, rich in abundant nutrition, is the hat in the big fruit in the world four (Fructus Mali pumilae, Fructus Vitis viniferae, mandarin orange and Fructus Musae).Fructus Mali pumilae usually redness, but also have yellow and green.Fructus Mali pumilae is dicotyledon, spends light red or lilac red, and self-sterility mostly, needs cross-pollination, and fruit is grown by ovary and holder and formed.Sarcocarp is clear and melodious fragrant and sweet, can help digest.Rosaceae, Malus, arbor, up to 15 meters, trunk taupe, Lao Pi has irregular lobe or scales off, close raw fine hair during sprig children, after polish, puce.Phyllotaxy is single leaf alternate, oval to avette, long 4.9～10 centimetres, tip point, and edge has round crenation, and two sides hairiness during children, rear surface is smooth, dirty-green.Grey colour band blush, footpath 3～5 centimetres, bennet and calyx are respectively provided with canescence fine hair, calyx leaf length point, harbor, stamen 20, style 5, and self-sterility for most of kinds, need to plant pollinizers.Fruit is the most flat spherical, and more than 5 centimetres of footpath, all caving in two ends, often there is rib ridge end.Florescence 4～June；Really phase 7～November are the ripest.Fructus Mali pumilae is deciduous tree, has stronger polarity, and generally growth is vigorous, and trunk taupe, fruit is that a kind of fruit, such as apple, pear, etc., color and size are different because of kind.Happiness light, happiness subacidity is to neutral soil.Be most suitable for soil layer deep, be well-drained sand of ventilating rich in organic, cubsoil.
In recent years, the preventing and treating of Fructus Mali pumilae quarantine fungal disease, is still main controlling mode with chemical agent, but owing to Fructus Mali pumilae quarantine fungal disease is setting internal latent infection characteristic, make chemical pesticide cannot solve the Problems Concerning Their Recurrence of old scar so that this disease year after year, cannot effectively control so far；Simultaneously as the drug effect phase of chemical pesticide is shorter, access times and using dosage are continuing to increase, and fruit safety and Environmental security are constituted huge threat, directly affect China's Fructus Mali pumilae trade in the world.At present, the method for Biological control Fructus Mali pumilae quarantine fungal disease mainly includes Chinese herbal medicine extract preventing and treating, biocontrol fungi preventing and treating and utilizes agricultural antibiotic to prevent and treat.Therefore, use Biological control harmless treat, produce high-grade high-quality green or Organic Fruit, be increasingly becoming the fashion that people pursue.
Summary of the invention
It is an object of the invention to provide a kind of for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, with the problem solving to propose in above-mentioned background technology.
For achieving the above object, the present invention provides following technical scheme:
A kind of for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, include according to the raw material of weight portion: streptomyces microflavus 10-15 part, bacillus subtilis 10-15 part, chaetomium globosum 15-25 part, Brevibacillus laterosporus 8-10 part, bacillus amyloliquefaciens 15-20 part, Bacillus circulans 8-10 part, Trichoderma harzianum 10-20 part, Trichoderma asperellum 5-10 part, consor Trichoderma spp. 5-10 part, Paecilomyces lilacinus 5-10 part, ligustrin streptomycete 8-12 part, Gordonia bronchialis 8-12 part, Kang Naisishi bacillus 8-12 part, Tang oligosaccharide 5-10 part, oligochitosan citrate 10-15 part, silicon dioxide 20-25 part, calcium oxide 10-15 part, magnesium sulfate 3-5 part, ardealite 25-35 part.
As the further scheme of the present invention: include according to the raw material of weight portion: streptomyces microflavus 12 parts, bacillus subtilis 12 parts, chaetomium globosum 20 parts, Brevibacillus laterosporus 9 parts, bacillus amyloliquefaciens 17 parts, Bacillus circulans 9 parts, Trichoderma harzianum 15 parts, Trichoderma asperellum 7 parts, consor Trichoderma spp. 7 parts, Paecilomyces lilacinus 7 parts, ligustrin streptomycete 10 parts, Gordonia bronchialis 10 parts, Kang Naisishi bacillus 10 parts, Tang oligosaccharide 7 parts, oligochitosan citrate 12 parts, silicon dioxide 22 parts, calcium oxide 12 parts, 4 parts of magnesium sulfate, ardealite 30 parts.
As the present invention further scheme: the clump count >=2.0*10 of described streptomyces microflavus every gram8, the clump count >=2.0*10 of described bacillus subtilis every gram8。
As the present invention further scheme: the clump count >=2.0*10 of described streptomyces microflavus every gram9, the clump count >=2.0*10 of described bacillus subtilis every gram9。
As the present invention further scheme: the effective active bacterium quantity >=4*10 of described Brevibacillus laterosporus, bacillus amyloliquefaciens and Bacillus circulans9cfu/g。
As the present invention further scheme: the bacteria containing amount >=6*10 of described Trichoderma harzianum, Trichoderma asperellum, consor Trichoderma spp. and Paecilomyces lilacinus8cfu/g。
As the present invention further scheme: effective active bacterium quantity >=10 of described ligustrin streptomycete, Gordonia bronchialis and Kang Naisishi bacillus8cfu/g。
Compared with prior art, the invention has the beneficial effects as follows: the present invention can effectively suppress quarantine fungal disease, apple tree can be made healthy and strong, it is possible to reach the purpose of volume increase；Chaetomium globosum has the most antibacterial and bactericidal activity to Valsa mali, can control Valsa mali enduringly, thus reach thoroughly to control and effect a radical cure the purpose of canker of apple fruit；Need not any pesticide, nontoxic, harmless, pollution-free, noresidue, it is ensured that apple quality safety and the no pollution to environment, reduce the production cost of peasant, it is ensured that agricultural product quality and safety, Fructus Mali pumilae pure in mouth feel, sugariness are high.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, broadly fall into the scope of protection of the invention.
Embodiment 1
A kind of for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, include according to the raw material of weight portion: streptomyces microflavus 10 parts, bacillus subtilis 10 parts, chaetomium globosum 15 parts, Brevibacillus laterosporus 8 parts, bacillus amyloliquefaciens 15 parts, Bacillus circulans 8 parts, Trichoderma harzianum 10 parts, Trichoderma asperellum 5 parts, consor Trichoderma spp. 5 parts, Paecilomyces lilacinus 5 parts, ligustrin streptomycete 8 parts, Gordonia bronchialis 8 parts, Kang Naisishi bacillus 8 parts, Tang oligosaccharide 5 parts, oligochitosan citrate 10 parts, silicon dioxide 20 parts, calcium oxide 10 parts, 3 parts of magnesium sulfate, ardealite 25 parts.
Clump count >=the 2.0*10 of described streptomyces microflavus every gram8, the clump count >=2.0*10 of described bacillus subtilis every gram8。
The effective active bacterium quantity >=4*10 of described Brevibacillus laterosporus, bacillus amyloliquefaciens and Bacillus circulans9cfu/g。
Bacteria containing amount >=the 6*10 of described Trichoderma harzianum, Trichoderma asperellum, consor Trichoderma spp. and Paecilomyces lilacinus8cfu/g。
Effective active bacterium quantity >=10 of described ligustrin streptomycete, Gordonia bronchialis and Kang Naisishi bacillus8cfu/g。
Embodiment 2
A kind of for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, include according to the raw material of weight portion: streptomyces microflavus 1 part, bacillus subtilis 11 parts, chaetomium globosum 17 parts, Brevibacillus laterosporus 9 parts, bacillus amyloliquefaciens 16 parts, Bacillus circulans 9 parts, Trichoderma harzianum 12 parts, Trichoderma asperellum 6 parts, consor Trichoderma spp. 6 parts, Paecilomyces lilacinus 6 parts, ligustrin streptomycete 9 parts, Gordonia bronchialis 9 parts, Kang Naisishi bacillus 9 parts, Tang oligosaccharide 6 parts, oligochitosan citrate 11 parts, silicon dioxide 21 parts, calcium oxide 11 parts, 4 parts of magnesium sulfate, ardealite 27 parts.
Clump count >=the 2.0*10 of described streptomyces microflavus every gram9, the clump count >=2.0*10 of described bacillus subtilis every gram9。
The effective active bacterium quantity >=4*10 of described Brevibacillus laterosporus, bacillus amyloliquefaciens and Bacillus circulans9cfu/g。
Bacteria containing amount >=the 6*10 of described Trichoderma harzianum, Trichoderma asperellum, consor Trichoderma spp. and Paecilomyces lilacinus8cfu/g。
Effective active bacterium quantity >=10 of described ligustrin streptomycete, Gordonia bronchialis and Kang Naisishi bacillus8cfu/g。
Embodiment 3
A kind of for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, include according to the raw material of weight portion: streptomyces microflavus 12 parts, bacillus subtilis 12 parts, chaetomium globosum 20 parts, Brevibacillus laterosporus 9 parts, bacillus amyloliquefaciens 17 parts, Bacillus circulans 9 parts, Trichoderma harzianum 15 parts, Trichoderma asperellum 7 parts, consor Trichoderma spp. 7 parts, Paecilomyces lilacinus 7 parts, ligustrin streptomycete 10 parts, Gordonia bronchialis 10 parts, Kang Naisishi bacillus 10 parts, Tang oligosaccharide 7 parts, oligochitosan citrate 12 parts, silicon dioxide 22 parts, calcium oxide 12 parts, 4 parts of magnesium sulfate, ardealite 30 parts.
Clump count >=the 2.0*10 of described streptomyces microflavus every gram9, the clump count >=2.0*10 of described bacillus subtilis every gram9。
The effective active bacterium quantity >=4*10 of described Brevibacillus laterosporus, bacillus amyloliquefaciens and Bacillus circulans9cfu/g。
Bacteria containing amount >=the 6*10 of described Trichoderma harzianum, Trichoderma asperellum, consor Trichoderma spp. and Paecilomyces lilacinus8cfu/g。
Effective active bacterium quantity >=10 of described ligustrin streptomycete, Gordonia bronchialis and Kang Naisishi bacillus8cfu/g。
Embodiment 4
A kind of for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, include according to the raw material of weight portion: streptomyces microflavus 14 parts, bacillus subtilis 14 parts, chaetomium globosum 22 parts, Brevibacillus laterosporus 9 parts, bacillus amyloliquefaciens 18 parts, Bacillus circulans 9 parts, Trichoderma harzianum 17 parts, Trichoderma asperellum 8 parts, consor Trichoderma spp. 8 parts, Paecilomyces lilacinus 8 parts, ligustrin streptomycete 11 parts, Gordonia bronchialis 11 parts, Kang Naisishi bacillus 11 parts, Tang oligosaccharide 8 parts, oligochitosan citrate 14 parts, silicon dioxide 24 parts, calcium oxide 14 parts, 4 parts of magnesium sulfate, ardealite 32 parts.
Clump count >=the 2.0*10 of described streptomyces microflavus every gram8, the clump count >=2.0*10 of described bacillus subtilis every gram8。
The effective active bacterium quantity >=4*10 of described Brevibacillus laterosporus, bacillus amyloliquefaciens and Bacillus circulans9cfu/g。
Bacteria containing amount >=the 6*10 of described Trichoderma harzianum, Trichoderma asperellum, consor Trichoderma spp. and Paecilomyces lilacinus8cfu/g。
Effective active bacterium quantity >=10 of described ligustrin streptomycete, Gordonia bronchialis and Kang Naisishi bacillus8cfu/g。
Embodiment 5
A kind of for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, include according to the raw material of weight portion: streptomyces microflavus 15 parts, bacillus subtilis 15 parts, chaetomium globosum 25 parts, Brevibacillus laterosporus 10 parts, bacillus amyloliquefaciens 20 parts, Bacillus circulans 10 parts, Trichoderma harzianum 20 parts, Trichoderma asperellum 10 parts, consor Trichoderma spp. 10 parts, Paecilomyces lilacinus 10 parts, ligustrin streptomycete 12 parts, Gordonia bronchialis 12 parts, Kang Naisishi bacillus 12 parts, Tang oligosaccharide 10 parts, oligochitosan citrate 15 parts, silicon dioxide 25 parts, calcium oxide 15 parts, 5 parts of magnesium sulfate, ardealite 35 parts.
Clump count >=the 2.0*10 of described streptomyces microflavus every gram8, the clump count >=2.0*10 of described bacillus subtilis every gram8。
The effective active bacterium quantity >=4*10 of described Brevibacillus laterosporus, bacillus amyloliquefaciens and Bacillus circulans9cfu/g。
Bacteria containing amount >=the 6*10 of described Trichoderma harzianum, Trichoderma asperellum, consor Trichoderma spp. and Paecilomyces lilacinus8cfu/g。
Effective active bacterium quantity >=10 of described ligustrin streptomycete, Gordonia bronchialis and Kang Naisishi bacillus8cfu/g。
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, and without departing from the spirit or essential characteristics of the present invention, it is possible to realize the present invention in other specific forms.Therefore, no matter from the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is limited by claims rather than described above, it is intended that all changes fallen in the implication of equivalency and scope of claim included in the present invention.
In addition, it is to be understood that, although this specification is been described by according to embodiment, but the most each embodiment only comprises an independent technical scheme, this narrating mode of description is only for clarity sake, description should can also be formed, through appropriately combined, other embodiments that it will be appreciated by those skilled in the art that as an entirety, the technical scheme in each embodiment by those skilled in the art.
Claims (7)
1. one kind is used for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, it is characterized in that, include according to the raw material of weight portion: streptomyces microflavus 10-15 part, bacillus subtilis 10-15 part, chaetomium globosum 15-25 part, Brevibacillus laterosporus 8-10 part, bacillus amyloliquefaciens 15-20 part, Bacillus circulans 8-10 part, Trichoderma harzianum 10-20 part, Trichoderma asperellum 5-10 part, consor Trichoderma spp. 5-10 part, Paecilomyces lilacinus 5-10 part, ligustrin streptomycete 8-12 part, Gordonia bronchialis 8-12 part, Kang Naisishi bacillus 8-12 part, Tang oligosaccharide 5-10 part, oligochitosan citrate 10-15 part, silicon dioxide 20-25 part, calcium oxide 10-15 part, magnesium sulfate 3-5 part, ardealite 25-35 part.
The most according to claim 1 for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, it is characterized in that, include according to the raw material of weight portion: streptomyces microflavus 12 parts, bacillus subtilis 12 parts, chaetomium globosum 20 parts, Brevibacillus laterosporus 9 parts, bacillus amyloliquefaciens 17 parts, Bacillus circulans 9 parts, Trichoderma harzianum 15 parts, Trichoderma asperellum 7 parts, consor Trichoderma spp. 7 parts, Paecilomyces lilacinus 7 parts, ligustrin streptomycete 10 parts, Gordonia bronchialis 10 parts, Kang Naisishi bacillus 10 parts, Tang oligosaccharide 7 parts, oligochitosan citrate 12 parts, silicon dioxide 22 parts, calcium oxide 12 parts, 4 parts of magnesium sulfate, ardealite 30 parts.
The most according to claim 1 for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, it is characterised in that the clump count >=2.0*10 of described streptomyces microflavus every gram8, the clump count >=2.0*10 of described bacillus subtilis every gram8。
The most according to claim 2 for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, it is characterised in that the clump count >=2.0*10 of described streptomyces microflavus every gram9, the clump count >=2.0*10 of described bacillus subtilis every gram9。
The most according to claim 1 and 2 for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, it is characterised in that the effective active bacterium quantity >=4*10 of described Brevibacillus laterosporus, bacillus amyloliquefaciens and Bacillus circulans9cfu/g。
The most according to claim 1 for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, it is characterised in that the bacteria containing amount >=6*10 of described Trichoderma harzianum, Trichoderma asperellum, consor Trichoderma spp. and Paecilomyces lilacinus8cfu/g。
The most according to claim 1 for preventing and treating Fructus Mali pumilae quarantine fungal disease and promoting the microbial inoculum of volume increase, it is characterised in that effective active bacterium quantity >=10 of described ligustrin streptomycete, Gordonia bronchialis and Kang Naisishi bacillus8cfu/g。
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| CN106900767A (en) * | 2017-03-16 | 2017-06-30 | 新疆肥力沃生物工程有限公司 | Prevent and treat biological pesticide of apple tree canker and its preparation method and application |
| CN107384808A (en) * | 2017-09-04 | 2017-11-24 | 青岛农业大学 | Trichoderma asperellum TD3104 and its application in the microbial inoculum for suppressing phytopathogen is prepared |
| CN109134069A (en) * | 2018-08-21 | 2019-01-04 | 陕西赛恩农业科技股份有限公司 | A kind of microbe granular fertilizer and preparation method thereof |
| CN113930362A (en) * | 2021-11-01 | 2022-01-14 | 武汉生物工程学院 | Traditional Chinese medicine residue composite microbial agent, biological organic fertilizer and preparation method thereof |
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