CN105784645A - Photoacoustic combined real-time detection device - Google Patents
Photoacoustic combined real-time detection device Download PDFInfo
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- CN105784645A CN105784645A CN201410820574.3A CN201410820574A CN105784645A CN 105784645 A CN105784645 A CN 105784645A CN 201410820574 A CN201410820574 A CN 201410820574A CN 105784645 A CN105784645 A CN 105784645A
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Abstract
The invention discloses a photoacoustic combined real-time detection device, which comprises a piezo-electric crystal, a prism, a light source, a photoelectric detector and a frequency detector. The prism is arranged on the piezo-electric crystal. The light source is arranged on an incident light path, and the light source generates a light beam which passes through the prism and the piezo-electric crystal and then reaches an object to be measured on the surface of the piezo-electric crystal. The photoelectric detector is arranged on a reflected light path and is used for receiving the light beam reflected by the object to be measured. The frequency detector is electrically connected to the piezo-electric crystal and is used for driving the piezo-electric crystal to generate resonance and recording resonant frequency. The photoacoustic combined real-time detection device is suitable for joint detection of all optic reflection technology and piezoelectric sensor technology. By the photoacoustic combined real-time detection device, simultaneous detection of optical and acoustical signals of a sample is realized, errors caused by the case that test conditions are not identical when the optical and acoustical signals are separately detected are avoided, and specificity and accuracy of detection are raised.
Description
Technical field
The invention belongs to field of photodetection, specifically, relate to a kind of optoacoustic associating real-time detection apparatus.
Background technology
Biomolecule this dynamic process of ordered movement in vivo is the key of cellular signal transduction research, and multiple detection technique (such as, the enzyme linked immunological marking, fluorescent labeling, radioactive label etc.) is applied to the research of this process.But, these detection techniques more or less can cause the inactivation of part biological molecule because of the use of label.Unmarked real-time detection technique, it makes in-vitro simulated and detects biomolecule primitive reaction process and be possibly realized, the detection technique of the transducers such as its optically-based detecting element, piezoquartz and electrochemical electrode, wherein, for instance surface plasma resonance technology (SPR), elliptical polarization optical detection technique, QCM technology (QCM) and electrochemical workstation etc. are widely known by the people.
In order to expand the range of application of various detection technique, numerous associated detection techniques are also suggested in succession, for instance: the ellipse associated detection technique of-QCM partially, SPR-QCM associated detection technique and reflectometry-QCM associated detection technique.Wherein, the ellipse associated detection technique of-QCM partially and reflectometry-QCM associated detection technique are mainly based upon the normal incidence mode of light and detect, and detection sensitivity is relatively low.When solution state detects, it is necessary to sample cell contains solution so that sample consumption is big.This technology requires that detection sample solution is transparent, and testing result is subject to the interference of solution mobility；SPR-QCM associated detection technique it is crucial that the line polarized light of a certain fixed angle or wavelength enters into sensing element with the incidence of SPR angle, cause surface plasma body resonant vibration, now, intensity of reflected light there will be a depression, and record causes the angle of incidence of this depression or wavelength can obtain the sample message of sensitive component surfaces.But, the modulation of incident illumination is comprised the dual function of phase place and amplitude by sample, and SPR has only detected this element of amplitude and have ignored the sample message that comprises in phase place, and this makes this joint-detection device can not be pervasive in other optical detective technologies.Fortunately, the ellipse inclined art of total internal reflection is supplementing this leak just, and the ψ of its detection, Δ parameter contain amplitude information and the phase information of light respectively, and this makes detection technique sensitiveer.But, make light path become complicated the analysis of beam phase information, simultaneously as the birefringence effect of piezoquartz, further increase the complexity of light path.
Summary of the invention
In order to solve above-mentioned prior art Problems existing, it is an object of the invention to provide a kind of optoacoustic associating real-time detection apparatus, including piezoquartz, prism, light source, photodetector and frequency detector, described prism is arranged on described piezoquartz, described light source is arranged in optical path of incident light, it produces the light beam of light beam and its generation by described prism, expose on the object to be measured being arranged under described piezoquartz after described piezoquartz, described photodetector is arranged in reflection light light path, it is for receiving the light beam after described object reflection to be measured, described frequency detector electrically connects described piezoquartz, it is used for driving described piezoquartz to produce resonance, and record resonant frequency.
Further, the upper and lower surface of described piezoquartz is provided with metallic diaphragm.
Further, the cross sectional shape of described piezoquartz is circular.
Further, the cross sectional shape of described piezoquartz is round rectangle or orthogonal rectangle.
Further, the upper surface of described piezoquartz is provided with optical axis labelling.
Further, described optical axis is labeled as witness marking or can remember by mark.
The optoacoustic associating real-time detection apparatus of the present invention, joint-detection suitable in all optical reflection arts and Piezoelectric sensing technique, detect while being capable of optics and acoustic signal, because of the error of the incomplete same introducing of test condition when avoiding separate detection, improve specificity and the accuracy of detection, and utilize evanescent wave phase-sensitive nature to add optical detection sensitivity.Additionally, the optical parametric such as optical axis position of piezoquartz has been carried out measurement and labelling by the present invention, the differences between batches of chip are evaded so that it is suitable in phase measurement art and ionization meter art, for instance ellipse inclined art and surface plasma resonance technology (SPR).
Accompanying drawing explanation
What carry out in conjunction with the drawings is described below, and the above-mentioned and other side of embodiments of the invention, feature and advantage will become clearer from, in accompanying drawing:
Fig. 1 is the side view of optoacoustic associating real-time detection apparatus according to an embodiment of the invention；
Fig. 2 is the structural representation of piezoquartz according to an embodiment of the invention；
Fig. 3 is the structural representation of piezoquartz according to another embodiment of the present invention；
Fig. 4 is the structural representation of piezoquartz according to still another embodiment of the invention.
Detailed description of the invention
Hereinafter, with reference to the accompanying drawings to embodiments of the invention are described in detail.However, it is possible to implement the present invention in many different forms, and the present invention should not be construed as limited to the specific embodiment set forth here.On the contrary, it is provided that these embodiments are to explain principles of the invention and practical application thereof, so that others skilled in the art are it will be appreciated that various embodiments of the present invention and be suitable for the various amendments of specific intended application.
Fig. 1 is the side view of optoacoustic associating real-time detection apparatus according to an embodiment of the invention.Fig. 2 is the structural representation of piezoquartz according to an embodiment of the invention.
With reference to Fig. 1, optoacoustic associating real-time detection apparatus includes according to an embodiment of the invention: piezoquartz 10, prism 20, light source 30, photodetector 40 and frequency detector 50.
Specifically, the overall trapezoidal mesa-shaped of prism 20, its bottom surface is positioned on the upper surface of piezoquartz 10.Light source 30 is arranged on input path I, its incident beam producing incident beam and its generation exposes to object to be measured (such as along input path I by after prism 20, piezoquartz 10, there is the diaphragm of protein solution) 60, wherein, this object 60 to be measured can be fitted on the lower surface of piezoquartz 10 by the mode of absorption or other suitable type.This object 60 to be measured reflects exposing to incident beam thereon, thus producing reflection light beam.This reflection light beam is by entering in reflected light path R after piezoquartz 10, prism 20.Photodetector 40 is arranged on reflected light path R, and it is for receiving the reflection light beam after object 60 to be measured reflects.Frequency detector 50 is electrically connected to piezoquartz 10, and it resonates for driving pressure electric crystal 10, and the resonant frequency that record piezoquartz 10 is when resonance.
Additionally, in the present embodiment, the upper and lower surface of piezoquartz 10 is provided with metallic diaphragm 70.So, when incident beam enters metallic diaphragm 70 (it is the medium of relatively low-refraction) from piezoquartz 10 (it is the medium of high index), if angle of incidence is just near critical angle (namely the critical angle of total reflection occurs), the interface of the lower surface of metallic diaphragm 70 and piezoquartz 10 can produce amplitude by the evanescent wave of exponential damping, this evanescent wave is perpendicular to interface and propagates to the direction of metallic diaphragm 70, propagate certain distance back reflection and return to piezoquartz 10, to form reflection light beam.When this evanescent wave runs into object 60 to be measured in communication process, object 60 to be measured will be subject to debug, there is corresponding change in its amplitude and phase place, simultaneously, the surface plasma resonance effect that the light beam entrance metallic diaphragm 70 of fixed wave length causes makes the amplitude generation greater attenuation of reflection light beam, so considerably increases detection sensitivity.So, by catching the signal of reflection light beam, just can measure the optical parametric of object 60 to be measured, such as thickness and complex refractivity index etc., wherein, to the detection sensitivity of thickness up to 0.1nm.
Frequency detector 50 uses piezoquartz 10 as crystal oscillator, and it is by detecting the change of piezoquartz 10 frequency of vibration, thus the change of the quality of the object to be measured 60 measured on the lower surface fitting in piezoquartz 10.But piezoquartz 10 is anisotropic medium, has birefringent phenomenon, the direction of vibration of its e light and the direction of propagation are subject to the tremendous influence of optical axis and angle of incidence.When piezoquartz 10 occurs slight movement will cause the huge change of emergent light character, detection process causes system mistake unavoidably.In order to avoid this system mistake, in the present embodiment, piezoquartz 10 being processed into circular sheet-like structures, namely the cross sectional shape of piezoquartz 10 is circular.
As other embodiments of the present invention, with reference to Fig. 3 and Fig. 4, piezoquartz 10 being processed into round rectangle laminated structure or orthogonal rectangle laminated structure, namely the cross sectional shape of piezoquartz 10 is round rectangle or orthogonal rectangle.
It addition, for the optical axis determining piezoquartz 10 such that it is able to duplicate detection result, the upper surface of piezoquartz 10 is provided with optical axis labelling 80, wherein, this optical axis labelling 80 is witness marking or can remember by mark.In the present embodiment, described witness marking refers to the labelling that human eye can observe directly；And described can mark note refer to the labelling that can be detected by detection instrument etc..
In sum, optoacoustic associating real-time detection apparatus according to an embodiment of the invention, joint-detection suitable in all optical reflection arts and Piezoelectric sensing technique, detect while being capable of optics and acoustic signal, because of the error of the incomplete same introducing of test condition when avoiding separate detection, improve specificity and the accuracy of detection, and utilize evanescent wave phase-sensitive nature to add optical detection sensitivity.In addition, optoacoustic associating real-time detection apparatus according to an embodiment of the invention, the optical parametric such as optical axis position of piezoquartz measurement and labelling have been carried out, the differences between batches of chip are evaded, it is made to be applicable to phase measurement art and ionization meter art, for instance ellipse inclined art and surface plasma resonance technology (SPR).
Although illustrate and describing the present invention with reference to specific embodiment, but it should be appreciated by those skilled in the art that: when without departing from the spirit and scope of the present invention limited by claim and equivalent thereof, the various changes in form and details can be carried out at this.
Claims (6)
1. an optoacoustic associating real-time detection apparatus, it is characterised in that include piezoquartz, prism, light source, photodetector and frequency detector,
Described prism is arranged on described piezoquartz,
Described light source is arranged in optical path of incident light, and its light beam producing light beam and its generation is arranged on the object to be measured of described piezoelectric crystal surface by exposing to after described prism, described piezoquartz,
Described photodetector is arranged in reflection light light path, and it is for receiving the light beam after described object reflection to be measured,
Described frequency detector electrically connects described piezoquartz, and it is used for driving described piezoquartz to produce resonance and record resonant frequency.
2. optoacoustic according to claim 1 associating real-time detection apparatus, it is characterised in that be provided with metallic diaphragm in the upper and lower surface of described piezoquartz.
3. optoacoustic according to claim 1 and 2 associating real-time detection apparatus, it is characterised in that the cross sectional shape of described piezoquartz is circular.
4. optoacoustic according to claim 1 and 2 associating real-time detection apparatus, it is characterised in that the cross sectional shape of described piezoquartz is round rectangle or orthogonal rectangle.
5. optoacoustic according to claim 1 associating real-time detection apparatus, it is characterised in that be provided with optical axis labelling on the upper surface of described piezoquartz.
6. optoacoustic according to claim 5 associating real-time detection apparatus, it is characterised in that described optical axis is labeled as witness marking or can remember by mark.
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| CN201410820574.3A CN105784645A (en) | 2014-12-25 | 2014-12-25 | Photoacoustic combined real-time detection device |
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| CN201410820574.3A CN105784645A (en) | 2014-12-25 | 2014-12-25 | Photoacoustic combined real-time detection device |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198400A (en) * | 2016-08-29 | 2016-12-07 | 苏州奥普特克自动化科技有限公司 | A kind of optoacoustic associating high flux real-time detecting system |
| CN110168350A (en) * | 2016-09-19 | 2019-08-23 | 密执安州立大学董事会 | Using the multi-mode biosensor with acoustic detector of integrated optical interferometry |
| CN110398461A (en) * | 2019-07-11 | 2019-11-01 | 上海交通大学 | A kind of three-D imaging method for realizing dendrite in lithium metal battery using photoacoustic imaging |
| CN114207406A (en) * | 2019-04-12 | 2022-03-18 | 阿韦技术有限责任公司 | Multi-modal characterization of fluid using a single droplet |
| CN115065415A (en) * | 2022-04-24 | 2022-09-16 | 南昌航空大学 | Method for obtaining micro-mass spectrum based on modulated optical signal |
-
2014
- 2014-12-25 CN CN201410820574.3A patent/CN105784645A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198400A (en) * | 2016-08-29 | 2016-12-07 | 苏州奥普特克自动化科技有限公司 | A kind of optoacoustic associating high flux real-time detecting system |
| CN110168350A (en) * | 2016-09-19 | 2019-08-23 | 密执安州立大学董事会 | Using the multi-mode biosensor with acoustic detector of integrated optical interferometry |
| CN114207406A (en) * | 2019-04-12 | 2022-03-18 | 阿韦技术有限责任公司 | Multi-modal characterization of fluid using a single droplet |
| CN110398461A (en) * | 2019-07-11 | 2019-11-01 | 上海交通大学 | A kind of three-D imaging method for realizing dendrite in lithium metal battery using photoacoustic imaging |
| CN115065415A (en) * | 2022-04-24 | 2022-09-16 | 南昌航空大学 | Method for obtaining micro-mass spectrum based on modulated optical signal |
| CN115065415B (en) * | 2022-04-24 | 2023-04-11 | 南昌航空大学 | Method for obtaining micro-mass spectrum based on modulated optical signal |
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