CN105779327B - One bacillus and culture medium and in alkaline ore dressing wastewater treatment reduce pH value method - Google Patents
One bacillus and culture medium and in alkaline ore dressing wastewater treatment reduce pH value method Download PDFInfo
- Publication number
- CN105779327B CN105779327B CN201410826804.7A CN201410826804A CN105779327B CN 105779327 B CN105779327 B CN 105779327B CN 201410826804 A CN201410826804 A CN 201410826804A CN 105779327 B CN105779327 B CN 105779327B
- Authority
- CN
- China
- Prior art keywords
- bacillus
- waste water
- culture
- follows
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Treatment Of Water By Oxidation Or Reduction (AREA)
Abstract
The present invention provides a kind of bacillus, the bacterium classification title are as follows: bacillus (Bacillus sp.) Biometek-T4, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are as follows: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, preservation date: on January 10th, 2013, deposit number: CGMCC No.7118.The bacterium can be seeded to ozone oxidation-carrier biofilm reactor, using microbe films osculation oxygenation method Beneficiation Wastewater, organic acid can be produced by fermentation during handling flotation waste water reduces pH value of waste water, so that zinc recovering and reutilizing waste water is selected to select lead process, it realizes the treatment for reuse of flotation waste water, realizes energy-saving and emission-reduction.
Description
Technical field
The present invention relates to microorganisms technical fields.More particularly to a kind of bacillus (Bacillus sp.)
Biometek-T4, culture medium, cultural method and the side for reducing pH during handling alkaline beneficiation wastewater using the bacterium
Method.
Background technique
Environmental statistics annual report data are shown within 2010, China nonferrous metal mine mining and ore dressing process heavy metals emission amount Zhan Chongjin
Belong to the 41.6% of total emission volumn.Polymetallic ore mountain beneficiation wastewater discharge amount accounts for about non-ferrous metal ore waste water 30%.With lead zinc sulphur
For changing mine, handling ore per ton needs water consumption 4-10m3.In conventional lead zinc sulfide minerals flotation separation process, sulfuric acid is added first
Zinc inhibits zincblende, adds the collecting agents diffeential floatation galena such as diethyldithiocarbamate, operates pH value in 8-9.Lead tailing is selected to add respectively
Add the medicaments such as copper sulphate and xanthate to activate collecting zincblende, operates pH value in 11-12.Due to diffeential floatation two stages regime of agent
Difference, pH value is different, and choosing can be seriously affected if untreated direct reuse containing heavy metals such as zinc, copper, lead by selecting in zinc waste water
Mine index, and then effect on environment is serious for outlet.Influence the key factor for selecting zinc waste water recycling to select lead process first is that pH, because
This, produces organic acid using fermentation in antimicrobial treatment process to reduce pH value of waste water, it will help realization selects zinc waste water recycling to select
Lead process realizes reuse before the factory of beneficiation wastewater, energy-saving and emission-reduction.
Summary of the invention
The first purpose of the invention is to provide one plant of efficient Bacillus, it is useless which can effectively reduce processing
Water pH value.
A second object of the present invention is to provide the culture mediums that the bacterium is cultivated in a kind of available, identification.
Third object of the present invention is to provide the culture mediums that one kind can be enriched with, be separately cultured the bacterium.
Fourth object of the present invention is to provide a kind of method for handling alkaline beneficiation wastewater using the bacterium and reducing pH value.
The purpose of the present invention is what is be achieved through the following technical solutions:
The present invention provides a kind of bacillus, the bacterium classification title are as follows: bacillus (Bacillus sp.)
Biometek-T4, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are as follows: Beijing
The institute 3 of Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica, preservation date: on January 10th, 2013, deposit number
Are as follows: CGMCC No.7118.
The culture medium of the bacillus, the formula of the culture medium are as follows: 2g yeast powder, 2g can are cultivated for obtaining, identifying
Soluble starch, 1g glucose, 0.5gZnCl2、0.3g CuCl2, 15g agarose and 1000mL distilled water, pH 7.0, hereinafter referred to as
For solid medium.
For being enriched with, being separately cultured the culture medium of the bacillus, the formula of the culture medium are as follows: 2g yeast powder, 2g can
Soluble starch, 1g glucose, 0.5gZnCl2、0.3g CuCl2With 1000mL distilled water, pH 7.0, hereinafter referred to as Liquid Culture
Base.
The Bacillus is inoculated with into 1L Liquid Culture by the enrichment of the bacillus, isolated culture method
In base, under 20-35 DEG C of cultivation temperature, shaking table culture 3-5 days.
A method of using heavy metal ion in bacillus removal beneficiation wastewater, by the strain of the bacillus,
It after carrying out enrichment culture using the method, is seeded in ozone oxidation-carrier biofilm reactor, inoculum concentration is every cube
(connect bacteria concentration is 10 to meter Ti Ji 2.5-3L8-109Cfu/mL), using microbe films osculation oxygenation method Beneficiation Wastewater.
The ozonation aerated amount of the ozone oxidation-carrier biofilm reactor is 300mL/min, and the processing time is 0.5-1
Hour.
The filler of the ozone oxidation-carrier biofilm reactor is selected from commercially available water process active carbon.
The beneficial effects of the present invention are:
The present invention provides one plant of bacterial strain Biometek-T4, which can effectively reduce flotation waste water pH value, thus favorably
In the treatment for reuse for realizing flotation waste water, energy-saving and emission-reduction are realized.
Detailed description of the invention
Fig. 1 is the stereoscan photograph of strain of the present invention.
Fig. 2 is cultivation results of the strain of the present invention under different growth temperatures.
Fig. 3 is that pH reduces situation during strain of the present invention handles waste water.
Specific embodiment
The present invention is described in further details below in conjunction with specific example.
Bacillus according to the present invention has carried out preservation, bacterium classification title are as follows: bacillus (Bacillus
Sp.) Biometek-T4, depositary institution are as follows: China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are as follows:
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, preservation day are as follows: protect on January 10th, 2013
Hiding number are as follows: CGMCC No.7118.
Embodiment 1: the acquisition of bacillus
The cultural method of bacillus of the present invention is as follows:
(1) solid medium for obtaining, identifying culture bacillus is prepared first: weighing 2g yeast powder, and 2g solubility is formed sediment
Powder, 1g glucose are added in 1000mL distilled water, adjust pH to 7.0,500mg ZnCl is then added2、300mg CuCl2, 15g fine jade
Lipolysaccharide, every 10mL falls one flat plate after sterilizing 25 minutes at 121 DEG C.
(2) to 100mL acquisition from the Guangxi dressing plant Che He flotation waste water water sample be added 0.3g yeast powder, 30 DEG C,
150rpm shake culture 1 week, bacterial growth situation was judged using microscope detection；
(3) light absorption value of the spectrophotometer measurement inoculum at 600nm is utilized, the numerical value of obtained OD600 is such as
Fruit shows that bacterium is in the logarithmic growth phase of vigorous growth, can carry out next step operation between 0.6-0.8；
(4) it is washed by the bacteriological filter in above-mentioned culture solution, and by bacterium with 20mL sterile distilled water using 0.2 μm of filter membrane
Under.It is inoculated in respectively with the inoculum concentration of the volume 5% relative to prepared culture medium in the culture medium of multiple steps (1) preparation,
30 DEG C of degree are cultivated, and are arranged and are not inoculated with the i.e. blank control (CK) of control.Bacterial growth feelings are observed in 100rpm culture after two weeks
Condition.Liquid Culture base fluid gradient dilution 1,2,3,4,5 (is respectively corresponded 10-1, 10-2, 10-3, 10-4, 10-5), 100 μ L are taken respectively
Dilution is coated on the plate of solid medium, and 30 DEG C are cultivated three days.It chooses single colonie and carries out further plate streaking, separate
To single colonie.It after picking single colonie, is transferred in new solid medium tablets, carries out separation training using plate streaking partition method
It supports, until obtaining single colonie.
Embodiment 2: the identification of bacillus
(1) fluid nutrient medium is prepared:
2g yeast powder, 2g soluble starch are weighed, 1g glucose is added in 1000mL distilled water, adjusts pH to 7.0, then adds
Enter 500mg ZnCl2、300mg CuCl2, sterilize 25 minutes at 121 DEG C.
(2) strain is identified
With scanning electron microscopic observation thalli morphology, thalli morphology is as shown in Figure 1.
Strain is analyzed and identified with 16S rDNA clone library technology.Single colonie is utilized into gained bacterium after fluid nutrient medium culture
Liquid 1mL is centrifuged to obtain bacterium mud, extracts total DNA, expands 16S using round pcr with prokaryotes universal primer 530f and 1490r
RDNA segment.PCR product is connect with the T-easy carrier of Promega after purification, converts bacillus coli DH 5 alpha.The white bacterium of picking
It falls and determines that positive bacterium colony tentatively judges that separated strain has been pure training through digestion with restriction enzyme parting by bacterium colony PCR
Object is supported, to 4 cloning and sequencings.Gained sequence relatively shows that the bacterial strain is that Bacillus belongs to bacterium through Blast, is named as
Biometek-T4。
(3) bacterial strain optimum growth temperature is determined
It will identify that strain inoculated into fluid nutrient medium, by temperature gradient shaking table culture, determines the most adaptability of bacterium
Long temperature, growth temperature section is as shown in Fig. 2, be shown in 20-35 DEG C of bacterial multiplication ability highest.
Embodiment 3: the enrichment of bacillus is separately cultured
The Bacillus of purifying is inoculated with the inoculum concentration of the volume 5% relative to prepared fluid nutrient medium into liquid
In body culture medium, under 20-35 DEG C of cultivation temperature, acquisition bud is collected by centrifugation by centrifuge in 100rpm shaking table culture 3-5 days
Spore bacillus.
Embodiment 4
By the bacillus Biometek-T4 of enrichment culture, it is seeded in ozone oxidation-carrier biofilm reactor, connects
Kind amount is every cubic metre of filler about 2.5L, bacteria concentration 108Cfu/mL, and tested using the practical beneficiation wastewater in the dressing plant Che He
Card, ozonation aerated amount are 300mL/min, and the processing time is 0.5 hour, and filler is water process active carbon, and each stage is discharged pH
Value is shown in Fig. 3.From figure 3, it can be seen that there is significantly pH value of waste water by filler biological film process after inoculation Biometek-T4
Decline has reached the pH index that lead process is selected in reflux.Meanwhile activated carbon adsorption heavy metal, the heavy metal of waste water can also be made to contain
Amount reaches the index that lead process is selected in reflux.
One plant of bacterial strain Biometek-T4 provided by the invention, can effectively reduce flotation waste water pH value, be conducive to select zinc waste water
Lead process is selected in reuse, to reach the treatment for reuse of flotation waste water, realizes energy-saving and emission-reduction.
Claims (5)
1. a kind of bacillus, which is characterized in that its specific name are as follows: bacillus (Bacillus sp.) Biometek-T4,
Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are as follows: Chaoyang District, Beijing City North Star west
The institute 3 of road 1, Institute of Microorganism, Academia Sinica, preservation date: on January 10th, 2013, deposit number: CGMCC
No.7118。
2. the Enrichment culture method of bacillus as described in claim 1, which is characterized in that connect the Bacillus
Kind enters in culture medium, under 20-35 DEG C of cultivation temperature, shaking table culture 3-5 days；
The formula of the culture medium are as follows: 2g yeast powder, 2g soluble starch, 1g glucose, 0.5g ZnCl2、0.3g CuCl2With
1000mL distilled water, pH 7.0.
3. a kind of method for being used in alkaline ore dressing wastewater treatment reduce pH value using bacillus described in claim 1,
It is characterized in that, after the bacillus is carried out enrichment culture using method according to claim 2, is seeded to ozone oxygen
In change-carrier biofilm reactor, inoculum concentration is every cubic meter volume 2.5L-3L, and connecing bacteria concentration is 108-109Cfu/mL is used
Microbe films osculation oxygenation method Beneficiation Wastewater.
4. method as claimed in claim 3, which is characterized in that the ozone of the ozone oxidation-carrier biofilm reactor exposes
Tolerance is 300mL/min, and the processing time is 0.5-1 hours.
5. method as claimed in claim 3, which is characterized in that the filler of the ozone oxidation-carrier biofilm reactor selects
From commercially available water process active carbon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410826804.7A CN105779327B (en) | 2014-12-25 | 2014-12-25 | One bacillus and culture medium and in alkaline ore dressing wastewater treatment reduce pH value method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410826804.7A CN105779327B (en) | 2014-12-25 | 2014-12-25 | One bacillus and culture medium and in alkaline ore dressing wastewater treatment reduce pH value method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105779327A CN105779327A (en) | 2016-07-20 |
| CN105779327B true CN105779327B (en) | 2019-10-01 |
Family
ID=56389383
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410826804.7A Active CN105779327B (en) | 2014-12-25 | 2014-12-25 | One bacillus and culture medium and in alkaline ore dressing wastewater treatment reduce pH value method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105779327B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111378592B (en) * | 2018-12-27 | 2022-03-11 | 有研资源环境技术研究院(北京)有限公司 | Bacillus licheniformis and method for treating malodorous organic wastewater by using same to purify water |
| CN115583732A (en) * | 2022-09-27 | 2023-01-10 | 江西盖亚环保科技有限公司 | Beneficiation wastewater treatment method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104058560A (en) * | 2014-07-16 | 2014-09-24 | 长春黄金研究院 | Nonferrous mine wastewater treatment method |
-
2014
- 2014-12-25 CN CN201410826804.7A patent/CN105779327B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104058560A (en) * | 2014-07-16 | 2014-09-24 | 长春黄金研究院 | Nonferrous mine wastewater treatment method |
Non-Patent Citations (1)
| Title |
|---|
| 车河选厂硫化矿浮选废水处理及回用研究;王自超;《中国优秀硕士学位论文全文数据库 工程科技I辑》;20130115;摘要，第1.4、2.4.3节，图2.8 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105779327A (en) | 2016-07-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104673710B (en) | Rhodococcus strain and its application | |
| CN108587915B (en) | Chlorella W5 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN106867921B (en) | Acidithiobacillus and method for treating acid mine wastewater and recycling iron resources by using same | |
| CN102337229A (en) | Separation of heavy metal tolerant bacteria and application thereof in reclamation of gangue | |
| CN108546649B (en) | Chlorella W2 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN114703095B (en) | Pseudomonas adulthood and application thereof in field of sewage and wastewater purification | |
| CN109576159B (en) | Chlorella W4 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN104450544B (en) | A kind of method of heavy metal ion in bacillus and its removal beneficiation wastewater | |
| CN105779327B (en) | One bacillus and culture medium and in alkaline ore dressing wastewater treatment reduce pH value method | |
| Karnachuk et al. | Bacteria of the sulfur cycle in the sediments of gold mine tailings, Kuznetsk Basin, Russia | |
| JP2022512455A (en) | Use in the treatment of membrane concentrates of Pseudomonas balealica and its debris leachate | |
| CN109576160B (en) | Chlorella W3 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN103833144A (en) | Method for removing heavy metal ions in water by using fermentation broth of bacterium producing flocculant | |
| CN106635854A (en) | Oligographic bacterium composition and application of oligographic bacterium composition in inhibition on generation of jarosite in biological leaching | |
| CN103013866A (en) | Nitrate-resistant sulfate reducing bacteria | |
| CN111378592B (en) | Bacillus licheniformis and method for treating malodorous organic wastewater by using same to purify water | |
| WO2017035856A1 (en) | Selenophilic microbe wautersiella enshiensis ylx-1 and application thereof | |
| CN109576161B (en) | Chlorella W1 capable of removing heavy metals in water with high heavy metal content and application thereof | |
| CN113249276B (en) | Bacillus cereus and application thereof | |
| CN103421702B (en) | Bacteria Lysinibacillus sp. for adsorbing gold and antimony | |
| CN104450545B (en) | A kind of method of bacillus, culture medium and its Beneficiation Wastewater | |
| CN108102988B (en) | Heavy metal resistant bacillus, microbial preparation and application | |
| CN103898001B (en) | A kind of heterotrophism acidophilic bacteria and the method utilizing the suppression acid wastewater in mine generation of this bacterium | |
| CN102399721B (en) | Marine sulfur oxidizing halothiobacillus bacterial strain HGMS18 (Homeotic Genic Male Sterile) and application thereof | |
| CN108265013B (en) | Sulfate reducing bacteria and process for recovering valuable metals in zinc smelting wastewater by using same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20190626 Address after: 101407 No. 11 Xingke East Street, Yanqi Economic Development Zone, Huairou District, Beijing Applicant after: Research Institute of engineering and Technology Co., Ltd. Address before: No. 2, Xinjie street, Xicheng District, Beijing, Beijing Applicant before: General Research Institute for Nonferrous Metals |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210402 Address after: 101407 No. 11 Xingke East Street, Yanqi Economic Development Zone, Huairou District, Beijing Patentee after: Youyan resources and Environment Technology Research Institute (Beijing) Co.,Ltd. Address before: 101407 No. 11 Xingke East Street, Yanqi Economic Development Zone, Huairou District, Beijing Patentee before: YOUYAN ENGINEERING TECHNOLOGY RESEARCH INSTITUTE Co.,Ltd. |