CN105779289A - Enzymolysis device of extraction process of polysaccharide in peanut meal - Google Patents

Enzymolysis device of extraction process of polysaccharide in peanut meal Download PDF

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Publication number
CN105779289A
CN105779289A CN201610355229.6A CN201610355229A CN105779289A CN 105779289 A CN105779289 A CN 105779289A CN 201610355229 A CN201610355229 A CN 201610355229A CN 105779289 A CN105779289 A CN 105779289A
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container
reaction vessel
plate
heating
temperature
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CN201610355229.6A
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CN105779289B (en
Inventor
邓鹏�
巩东营
李志国
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Shandong Daofang Biotechnology Co ltd
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Institute of Agro Food Science and Technology of Shandong Academy of Agricultural Sciences
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Thermal Sciences (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

The invention discloses an enzymolysis device of the extraction process of polysaccharide in peanut meal. The enzymolysis device comprises a heating container, a reaction container, a container cover, a water release valve and a temperature control heating element. The reaction container is arranged in the heating container. The temperature control heating element is arranged in the heating container. The heating container and the reaction container are covered with the container cover. One end of the water release valve is communicated with the reaction container, and the other end of the water release valve is arranged outside the heating container. During enzymolysis reaction, the heating container is filled with water, the temperature is adjusted by a control circuit to the required temperature, when the water temperature reaches the required temperature, a heating resistor stops heating, a reaction solution is added into the reaction container, the temperature is kept by the container cover, the water temperature is controlled by the control circuit, heating is conducted when the water temperature drops, a controllable constant-temperature environment is provided for enzymolysis reaction, the water temperature is adjusted to the boiling water temperature during enzyme deactivation reaction, enzyme deactivation reaction can be directly conducted, and operation time is saved.

Description

The enzymolysis device of polysaccharide extracting process in a kind of Semen arachidis hypogaeae dregs
Technical field
The present invention relates to the enzymolysis device of polysaccharide extracting process in a kind of Semen arachidis hypogaeae dregs.
Background technology
In the process extracting peanut polysaccharide from hot pressed peanut meal, Semen arachidis hypogaeae dregs enzyme extracts reaction, and hydrolysis temperature is 30~50 DEG C, pH4~6, enzymolysis time is 1.5h~5h, after enzymolysis terminates, will carry out enzyme denaturing, reaction system is placed enzyme denaturing 4~6min in 100 DEG C of boiling water baths, makes enzyme deactivation.And simply reaction vessel is placed in preprepared water temperature at present, and not carrying out Insulation, water temperature drop is very fast.In enzyme denaturing process, reflection container need to be placed in boiling water again, complex operation.
Summary of the invention
The technical problem to be solved in the present invention is: provide the enzymolysis device of polysaccharide extracting process in a kind of Semen arachidis hypogaeae dregs, for the environment of polysaccharide enzyme digestion reaction one the controlled constant temperature of offer of Semen arachidis hypogaeae dregs.
In order to solve above-mentioned technical problem, the present invention includes adding heat container, reaction vessel, container cover, discharging valve and temperature control heating element;Reaction vessel is placed in and adds in heat container, and temperature control heating element is arranged on heating internal tank, and container cover covers and adds on heat container and reaction vessel, and discharging valve one end connects with reaction vessel, and the other end is in the outside adding heat container.
For the ease of the placement of temperature control heating element and reaction vessel, present invention additionally comprises dividing plate, dividing plate has through hole, dividing plate is connected with heating container inner wall, and reaction vessel is on the upside of dividing plate, and described temperature control heating element is on the downside of dividing plate.
In order to reduce the loss adding heat container interior-heat amount, container cover of the present invention includes inner cap, enclosing cover and hands handle, handle is connected through enclosing cover with inner cap, enclosing cover includes intermediate plate, ring flat-plate and connection bucket, connect bucket upper end to be connected with intermediate plate, connect bucket lower end to be connected with ring flat-plate, add heat container and reaction vessel is the staving that upper end is uncovered, the upper port of reaction vessel is higher than the upper port adding heat container, when container cover covers reaction vessel and adds heat container, intermediate plate covers reaction vessel, ring flat-plate covers and adds heat container, inner cap extend into reaction vessel interior and fits with reaction vessel inwall.
In order to convenient, heating water temperature is controlled, temperature control heating element of the present invention includes liquid crystal indicator, temperature sensor, control circuit and heating resistor, liquid crystal indicator is connected with temperature sensor and control circuit respectively, control circuit is connected with heating resistor, temperature sensor and heating resistor are placed in and add in heat container, and control circuit and liquid crystal indicator are placed on heating container outer wall.
In order to allow reaction solution all carry out enzyme digestion reaction under thermophilic environment, discharging valve of the present invention includes water pipe head, spool and joystick, joystick and water pipe head are rotationally connected and junction seals, spool is in inside water pipe head, spool includes fixing plate and adjustable plate, joystick one end is fixing with adjustable plate to be connected, the joystick other end is in the outside adding heat container, fixing plate fits tightly with adjustable plate, fixing plate there is water breach, adjustable plate is identical with fixing plate shape, the spool of discharging valve is positioned at heating container inside, in joystick rotation process, the water breach of crossing of adjustable plate communicates with the water breach existence of crossing of fixing plate and is not communicated with two states.
The invention has the beneficial effects as follows: the present invention is when carrying out enzyme digestion reaction, topped up with water in adding heat container, control circuit is utilized to be adjusted to temperature required, when water temperature reaches temperature required, heating resistor stops heating, reaction solution is joined in reaction vessel, and be incubated by container cover, water temperature is controlled by control circuit, is heated during water temperature drop, provides a controlled isoperibol for enzyme digestion reaction, when carrying out enzyme denaturing reaction, by lower water temperature to boiled water temperature, enzyme denaturing reaction can be made directly, save the operating time.
Accompanying drawing explanation
Fig. 1 is the enzymolysis device structural representation of polysaccharide extracting process in Semen arachidis hypogaeae dregs;
Fig. 2 is discharging valve fixed plate structure schematic diagram;
Fig. 3 is water drain valve structure schematic diagram;
The enzymolysis device circuit that Fig. 4 is polysaccharide extracting process in Semen arachidis hypogaeae dregs controls schematic diagram.
In figure: 1, container cover, 2, add heat container, 3, reaction vessel, 4, discharging valve, 5, dividing plate, 6, heating resistor, 7, electrical appliance kit, 1-1, enclosing cover, 1-2, handle, 1-3 inner cap, 4-1, fixing plate, 4-2, adjustable plate, 4-3, water pipe head, 4-4, joystick, A, temperature sensor, B, control circuit, C, liquid crystal indicator, D, heating resistor.
Detailed description of the invention
The enzymolysis device of polysaccharide extracting process in a kind of Semen arachidis hypogaeae dregs as shown in Figure 1, including container cover 1, add heat container 2, reaction vessel 3, discharging valve 4, dividing plate 5, heating resistor 6 and electrical appliance kit 7, add heat container 2 and reaction vessel 3 is the staving that upper end is uncovered, dividing plate 5 there is through hole, and it is fixed on the inwall adding heat container 2, heat container 2 will be added and be divided into upper and lower two parts, heating resistor 6 is in the downside of dividing plate 5, reaction vessel 3 is put on dividing plate 5, the upper port of reaction vessel 3 is higher than the upper port adding heat container 2, discharging valve 4 is arranged on the bottommost of reaction vessel 3, discharging valve 4 is through adding heat container 2, the outlet of discharging valve 4 is in the outside adding heat container 2.Container cover 1 includes enclosing cover 1-1, inner cap 1-3 and handle 1-2, handle 1-2 is connected with inner cap 1-3 by enclosing cover 1-1, when container cover 1 is covered at reaction vessel 3 and adds on heat container 2, enclosing cover 1-1 covers reaction vessel 3 simultaneously and adds heat container 2, and inner cap 1-3 extend into reaction vessel 3 inside and fits with reaction vessel 3 inwall.Electrical appliance kit 7 includes liquid crystal indicator C, control circuit B and enzymolysis device power supply as shown in Figure 4, is installed on the outer wall adding heat container 2.
Discharging valve 4 as shown in Figure 3, including fixing plate 4-1 and adjustable plate 4-2, water pipe head 4-3, joystick 4-4, joystick 4-4 and water pipe head 4-3 is rotationally connected and junction seals, fixing plate 4-1 and adjustable plate 4-2 is in inside water pipe head 4-3, the two fits tightly and shape is identical, adjustable plate 4-2 and joystick 4-4 is fixing to be connected, the adjustable plate 4-2 and fixing plate 4-1 of discharging valve are in the inner side adding heat container 2, fixing plate 4-1 as shown in Figure 2 there is water breach, in joystick 4-4 rotation process, the breach of fixing plate 4-1 communicates with the breach existence of adjustable plate 4-2 and is not communicated with two states.
The circuit of the enzymolysis device of polysaccharide extracting process in Semen arachidis hypogaeae dregs as shown in Figure 4, including temperature sensor A, control circuit B, liquid crystal indicator C and heating resistor D, when in Semen arachidis hypogaeae dregs, the enzymolysis device of polysaccharide extracting process works, control circuit B arranges required heating-up temperature, and shown by liquid crystal indicator C, temperature sensor A records current water temperature, signal is passed to liquid crystal indicator C and control circuit B, liquid crystal indicator C shows Current Temperatures, and control circuit B is by comparing Current Temperatures and arranging temperature and control the switch of heating resistor D.

Claims (5)

1. the enzymolysis device of polysaccharide extracting process in a Semen arachidis hypogaeae dregs, it is characterised in that: include adding heat container, reaction vessel, container cover, discharging valve and temperature control heating element;Reaction vessel is placed in and adds in heat container, and temperature control heating element is arranged on heating internal tank, and container cover covers and adds on heat container and reaction vessel, and discharging valve one end connects with reaction vessel, and the other end is in the outside adding heat container.
2. the enzymolysis device of polysaccharide extracting process in Semen arachidis hypogaeae dregs according to claim 1, it is characterised in that: also including dividing plate, dividing plate has through hole, dividing plate is connected with heating container inner wall, and reaction vessel is on the upside of dividing plate, and described temperature control heating element is on the downside of dividing plate.
3. the enzymolysis device of polysaccharide extracting process in Semen arachidis hypogaeae dregs according to claim 2, it is characterized in that: described container cover includes inner cap, enclosing cover and hands handle, handle is connected through enclosing cover with inner cap, enclosing cover includes intermediate plate, ring flat-plate and connection bucket, connect bucket upper end to be connected with intermediate plate, connect bucket lower end to be connected with ring flat-plate, add heat container and reaction vessel is the staving that upper end is uncovered, the upper port of reaction vessel is higher than the upper port adding heat container, when container cover covers reaction vessel and adds heat container, intermediate plate covers reaction vessel, ring flat-plate covers and adds heat container, inner cap extend into reaction vessel interior and fits with reaction vessel inwall.
4. the enzymolysis device of polysaccharide extracting process in Semen arachidis hypogaeae dregs according to claim 3, it is characterized in that: described temperature control heating element includes liquid crystal indicator, temperature sensor, control circuit and heating resistor, liquid crystal indicator is connected with temperature sensor and control circuit respectively, control circuit is connected with heating resistor, temperature sensor and heating resistor are placed in and add in heat container, and control circuit and liquid crystal indicator are placed on heating container outer wall.
5. the enzymolysis device of polysaccharide extracting process in any one Semen arachidis hypogaeae dregs according to claim 1-4, it is characterized in that: described discharging valve includes water pipe head, spool and joystick, joystick and water pipe head are rotationally connected and junction seals, spool is in inside water pipe head, spool includes fixing plate and adjustable plate, joystick one end is fixing with adjustable plate to be connected, the joystick other end is in the outside adding heat container, fixing plate fits tightly with adjustable plate, fixing plate there is water breach, adjustable plate is identical with fixing plate shape, the spool of discharging valve is positioned at heating container inside, in joystick rotation process, the water breach of crossing of adjustable plate communicates with the water breach existence of crossing of fixing plate and is not communicated with two states.
CN201610355229.6A 2016-05-26 2016-05-26 The enzymolysis device of polysaccharide extracting process in a kind of peanut meal Active CN105779289B (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201640415U (en) * 2010-03-05 2010-11-24 丛茜 Food fermentation container
CN103864954A (en) * 2014-04-09 2014-06-18 山东金胜粮油集团有限公司 Method for extracting peanut meal polysaccharide
CN104353512A (en) * 2014-09-30 2015-02-18 深圳市理邦精密仪器股份有限公司 All-surrounding type water bath circulating constant-temperature heating device
CN204685126U (en) * 2015-06-09 2015-10-07 孟如苗 A kind of efficient constant-temperature water-bath
CN204710377U (en) * 2015-04-30 2015-10-21 柯良斌 A kind of water bath with thermostatic control heater
CN205687947U (en) * 2016-05-26 2016-11-16 山东省农业科学院农产品研究所 The enzymolysis device of polysaccharide extracting process in a kind of Semen arachidis hypogaeae dregs

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201640415U (en) * 2010-03-05 2010-11-24 丛茜 Food fermentation container
CN103864954A (en) * 2014-04-09 2014-06-18 山东金胜粮油集团有限公司 Method for extracting peanut meal polysaccharide
CN104353512A (en) * 2014-09-30 2015-02-18 深圳市理邦精密仪器股份有限公司 All-surrounding type water bath circulating constant-temperature heating device
CN204710377U (en) * 2015-04-30 2015-10-21 柯良斌 A kind of water bath with thermostatic control heater
CN204685126U (en) * 2015-06-09 2015-10-07 孟如苗 A kind of efficient constant-temperature water-bath
CN205687947U (en) * 2016-05-26 2016-11-16 山东省农业科学院农产品研究所 The enzymolysis device of polysaccharide extracting process in a kind of Semen arachidis hypogaeae dregs

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Address after: No. 202, Gongye North Road, Jinan City, Shandong Province

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Address after: No. 16500 Longquan Road, Longshan Street, Zhangqiu District, Jinan City, Shandong Province, 250000 (No. 7 Longshan Flavor Creative Space)

Patentee after: Shandong longkuo kinetic energy transformation Technology Business Incubator Co.,Ltd.

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Patentee after: Shandong Daofang Biotechnology Co.,Ltd.

Country or region after: Zhong Guo

Address before: No. 16500 Longquan Road, Longshan Street, Zhangqiu District, Jinan City, Shandong Province, 250000 (No. 7 Longshan Flavor Creative Space)

Patentee before: Shandong longkuo kinetic energy transformation Technology Business Incubator Co.,Ltd.

Country or region before: Zhong Guo