CN105771004B - A kind of hydrogel, preparation method and its application - Google Patents
A kind of hydrogel, preparation method and its application Download PDFInfo
- Publication number
- CN105771004B CN105771004B CN201610133596.1A CN201610133596A CN105771004B CN 105771004 B CN105771004 B CN 105771004B CN 201610133596 A CN201610133596 A CN 201610133596A CN 105771004 B CN105771004 B CN 105771004B
- Authority
- CN
- China
- Prior art keywords
- hydrogel
- alt
- sodium alginate
- maleic anhydride
- vinyl ether
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/145—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/02—Inorganic materials
- A61L31/028—Other inorganic materials not covered by A61L31/022 - A61L31/026
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/042—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/048—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/06—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
- C08L5/04—Alginic acid; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0062—General methods for three-dimensional culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2513/00—3D culture
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Epidemiology (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Inorganic Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a kind of hydrogel, preparation method and its applications, hydrogel is to form network structure by poly- (methyl vinyl ether-alt- maleic anhydride), PEG and sodium alginate cross-linking, again with the sodium ion of metal cations ions displacement sodium alginate, a series of hydrogel of degrees of cross linking is obtained.Preparation method is: (1) preparing poly- (methyl vinyl ether-alt- maleic anhydride), sodium alginate, PEG aqueous solution；(2) aqueous solution of three kinds of raw materials is mixed, heating crosslinks reaction, obtains uniform, transparent film；(3) with the sodium ion of metal cation displacement sodium alginate, the hydrogel of the different degrees of cross linking is obtained, obtained hydrogel homogeneous transparent, moderate strength can be used as 3D culture timbering material and carry out cell culture.
Description
Technical field
The invention belongs to cell 3D to cultivate field, and in particular to a kind of hydrogel, preparation method and its application.
Background technique
Hydrogel material structure is clear, the repeatability with height, can by adjusting its chemically and physically chemical property,
The feature of fine simulation extracellular matrix (ECM).3D culture systems based on hydrogel, which have, provides internal like cell-cell
The potential that epimatrix interaction and cell-matrix stick.
It is used to prepare in three-dimensional stent material, natural material includes collagen, hyaluronic acid, matrigel, chitosan, Portugal
Glycan and alginic acid etc.；Synthetic material be mainly polyethylene glycol (PEG), polylactic acid (PLA), polylactic acid-glycollic acid (PLGA) and
Polyglycerol sebacate (PGS) and its derivative.But in material as above, the natural material of good biocompatibility is adjustable
Control property is not high, and the synthetic material that control is good, biocompatibility remain to be discussed.Therefore the composite wood of some natural-synthesis
Material comes into being, and other than significant mechanical property, also shows superior biocompatibility.Such as calcium-alginic acid/polypropylene
Amide hydrogel, synthesis can be realized by one-step method or two-step method, and one-step method, that is, all is at being grouped together mixing；Two-step method
It is first to synthesize sodium-alginic acid/polyacrylamide, then react with polyvalent cation.In addition there are also silk-Sodium Alginate Hydrogel Films,
Gelatin-Methacrylamide hydrogel and gelatin-polymethyl vinyl ether/maleic anhydride hydrogel etc..Poly- (methyl ethylene
Ether-alt- maleic anhydride) (PMVE-alt-MAH) and its poly- (methyl vinyl ether-alt- the maleic acid) (PMVE- of hydrolysate
Alt-MA) it is the high molecular material nontoxic to human body and animal, there is good chemical stability and biocompatibility.Benefit
The Modulatory character that the advantage of cell culture studies is its matrix solidity is carried out with hydrogel material, so that analog is various micro-
The requirement of environment.
Summary of the invention
For overcome the deficiencies in the prior art, the purpose of the present invention is to provide a kind of hydrogel, preparation method and its
Using, simple process is with short production cycle, and it is pollution-free, it is suitable for large-scale production；Gained hydrogel, uniform, moderate strength, has
Preferable biocompatibility can be used as cell 3D culture timbering material.
In order to achieve the above-mentioned object of the invention, technical solution provided by the invention is as follows:
Hydrogel forms network structure by poly- (methyl vinyl ether-alt- maleic anhydride), PEG and sodium alginate cross-linking,
Again with the sodium ion of metal cation displacement sodium alginate, a series of hydrogel of degrees of cross linking is obtained.
The preparation method of hydrogel, includes the following steps:
(1) aqueous solution of poly- (methyl vinyl ether-alt- maleic anhydride), sodium alginate, PEG are prepared respectively；
(2) poly- (methyl vinyl ether-alt- maleic anhydride) solution, sodium alginate aqueous solution are mixed with PEG aqueous solution
Uniformly, it heats, crosslinks reaction, obtain uniform, transparent film；
Crosslinking temperature is 80 DEG C, time 24-48h；
Poly- (methyl vinyl ether-alt- maleic anhydride) and PEG are 280:1~1120 with the molar ratio of-COOH and-OH:
1, mass ratio 8:1~16:1 of poly- (methyl vinyl ether-alt- maleic anhydride) and sodium alginate；
As the mass ratio 4:1 of P (MVE-alt-MA) and NaAlg, and poly- (methyl vinyl ether-alt- maleic anhydride) and
The molar ratio of PEG (in terms of-COOH:-OH) is 140:1, and resulting gel is in uneven, leukasmus is blocky, thus it is speculated that is network knot
Structure is too close, and each component cannot come into full contact with reaction, therefore need to reduce sodium alginate and the amount of PEG, if but the amount of PEG reach poly- (first
Base vinyl ethers-alt- maleic anhydride): when PEG=1120:1 or less, the intensity of gained hydrogel is too low；
The number-average molecular weight of poly- (methyl vinyl ether-alt- maleic anhydride) is the number-average molecular weight of 311000, PEG
It is 22000；
(3) preparing metal cationic aqueous solution, used metal salt are SrCl2·6H2O、CaSO4·2H2O or
FeCl3·6H2O, concentration are 0.01M~0.1M；
(4) film that step (2) obtains is soaked in the cationic aqueous solution of step (3), room temperature reaction is stayed overnight, in gel
Na+Ion exchange occurs with metal cation, then impregnates for 24 hours~48h, the deionization during which repeatedly renewed in deionized water
Water obtains P (MVE-alt-MA)/M of the different degrees of cross linking to remove unreacted metal cation+Alg hydrogel, M+For metal
Cation.
Hydrogel carries out the purposes of cell culture as 3D culture timbering material.
Alginic acid is a kind of linear copolymer, and comprising α-L- guluronic acid (unit) and beta-D-mannuronic acid, (M is mono-
Member).The Na ion of monovalence cannot be crosslinked alginic acid, however polyvalent cation crosslinking alginic acid passes through on carboxyl and alginic acid chain
Different units are in combination with Ca is combined with GG and GM sections, and Sr is only combined with GG sections.
The mechanical property of hydrogel depend on polyvalent cation with GG sections in alginic acid polymer, MM sections, GM sections mutual
Effect.The degree of cross linking of alginic acid depends on the characteristic of crosslinking agent ion, such as the charge and ionic radius of polyvalent cation.Using outer
Portion's solution and hydrogel carry out ion exchange, and multivalent ion is diffused into hydrogel from external solution, and Na+Ion is then from water-setting
Glue is diffused into external solution, to can get metal cation/seaweed acid gel.
Biocompatibility preferable poly- (methyl vinyl ether-alt- maleic anhydride) and sodium alginate etc. are selected in the application
Material to form different elasticity modulus by being mixed into for different metal cations, reach different hardnesses, to construct not
Same matrix solidity, to obtain ideal matrix research model in vivo.What is obtained is dual network structure hydrogel, and structure is equal
Even, moderate strength: first network is the chemical network being crosslinked by PEG, and the second network is crosslinked by different metal ions, this
Interpenetrating type network structure enhances the intensity of hydrogel.
Detailed description of the invention
Fig. 1 is the pictorial diagram of the preparation-obtained hydrogel of embodiment 1-3, wherein a, b, c column respectively correspond embodiment 1,
2,3, two samples of each column, it is seen that obtain being transparent, uniform hydrogel；
Fig. 2 be embodiment 1 obtain based on P (MVE-alt-MA)/SrAlg hydrogel as 3D bracket, the cell of culture
It grows picture (100 ×)；
Fig. 3 be embodiment 2 obtain based on P (MVE-alt-MA)/CaAlg hydrogel as 3D bracket, the cell of culture
It grows picture (100 ×)；
Fig. 4 be embodiment 3 obtain based on P (MVE-alt-MA)/FeAlg hydrogel as 3D bracket, the cell of culture
It grows picture (100 ×).
Specific embodiment
The explanation of raw material sources used by following instance: poly- (methyl vinyl ether-alt- maleic anhydride) (number-average molecular weight
311000) to be purchased from Aldrich；Polyethylene glycol is purchased from Xilong Chemical Co., Ltd (number-average molecular weight 22000)；
Strontium chloride, calcium sulfate, ferric trichloride are purchased from Sinopharm Chemical Reagent Co., Ltd..Sodium alginate (NaAlg) is purchased from Qingdao
Bright moon seaweed Group Co., Ltd.
Embodiment 1
A kind of preparation method of hydrogel, comprising the following steps:
(1) 0.1220g sodium alginate is dissolved in 18.7mL deionized water, ambient temperature overnight dissolution；By 1.9500g P
(MVE-alt-MAH) it is dissolved in 40mL deionized water, oil bath 2h under the conditions of 90 DEG C obtains the solution of clear；0.4910g
PEG is dissolved in 20mL deionized water, room-temperature dissolution；
(2) three of the above aqueous solution is mixed, 10min is stirred with 800rpm under room temperature, is placed in 80 DEG C of baking ovens and hands over
Connection for 24 hours, obtains one piece of uniform, transparent film；
The n-COOH/n-OH=560:1 of PMVE-alt-MA and PEG；
NaAlg amount is 1/16PMVE-alt-MA；
(3) 0.3999g strontium chloride is dissolved in 15mL deionized water, room-temperature dissolution, obtains strontium chloride aqueous solution；
(4) membrane pressure for obtaining step (2) is flat, is soaked in strontium chloride aqueous solution, reacts at room temperature for 24 hours, so that in gel
Na+With the Sr in aqueous solution2+Sufficient ion exchange is carried out, deionized water is then changed and impregnates for 24 hours, during which repeatedly more renew
Deionized water.P (MVE-alt-MA)/SrAlg hydrogel is finally obtained, is transparent, storage modulu 732.58Pa, swelling ratio
(g/g)=17.43.
(5) obtained water-setting rubber pulverizing, sterilizing are followed by 96 orifice plates with pulverizer, every 50 μ L of hole is added fresh
Culture solution is incubated overnight, inoculating cell, and inoculum concentration is 8000, every hole cell, cultivates 5d, observes cell growth status, inoculation
Cell is proliferated into cell aggregation by individual cells in hydrogel fragment, and live cell fluorescent dyeing also shows that cell activity.
Embodiment 2
It is as follows different from the content of embodiment 1:
(1) 0.2583g calcium sulfate is dissolved in 15mL deionized water, room-temperature dissolution, obtains calcium sulfate aqueous solution；
(4) membrane pressure for obtaining step (2) is flat, is soaked in calcium sulfate aqueous solution, carries out ion exchange, obtains P (MVE-
Alt-MA)/CaAlg hydrogel.White in appearance transparence, storage modulu 198.34Pa, swelling ratio (g/g)=17.06.
The cell of inoculation is proliferated into cell aggregation by individual cells in hydrogel fragment, and live cell fluorescent dyeing is also shown
Cell activity is shown.
Embodiment 3
The content that embodiment 3 is different from embodiment 1 is:
(3) 0.4054g ferric trichloride is dissolved in 15mL deionized water, and room-temperature dissolution obtains ferric chloride aqueous solutions；
(4) membrane pressure for obtaining step (2) is flat, is soaked in ferric chloride aqueous solutions, carries out ion exchange, obtains P
(MVE-alt-MA)/FeAlg hydrogel, appearance be in yellow transparent shape, storage modulu 10480.29Pa, swelling ratio (g/g)=
1.89。
The cell of inoculation is proliferated into cell aggregation by individual cells in hydrogel fragment, and live cell fluorescent dyeing is also shown
Cell activity is shown.
The technological parameter and raw material dosage of embodiment 4-7 is shown in Table 1:
Claims (4)
1. hydrogel, which is characterized in that formed by poly- (methyl vinyl ether-alt- maleic anhydride), PEG and sodium alginate cross-linking
Network structure, then the sodium ion with metal cation displacement sodium alginate, obtain a series of transparent aquagel of degrees of cross linking.
2. the preparation method of hydrogel described in claim 1, which comprises the steps of:
(1) aqueous solution of poly- (methyl vinyl ether-alt- maleic anhydride), sodium alginate, PEG are prepared respectively；
(2) poly- (methyl vinyl ether-alt- maleic anhydride) solution, sodium alginate aqueous solution are uniformly mixed with PEG aqueous solution,
Heating, crosslinks reaction, obtains uniform, transparent film；
The temperature of cross-linking reaction is 80 DEG C, reaction time 24-48h；
Poly- (methyl vinyl ether-alt- maleic anhydride) and PEG are 280:1 ~ 1120:1 with the molar ratio of-COOH and-OH, are gathered
The mass ratio of (methyl vinyl ether-alt- maleic anhydride) and sodium alginate is 8:1 ~ 16:1；
(3) preparing metal cationic aqueous solution, used metal salt are SrCl2 .6H2O、CaSO4 .2H2O or FeCl3 .6H2O is dense
Degree is 0.01M ~ 0.1M；
(4) film that step (2) obtains is soaked in the cationic aqueous solution of step (3), room temperature reaction is stayed overnight, the Na in gel+
Ion exchange occurs with metal cation, then impregnates for 24 hours ~ 48h in deionized water, the deionized water during which repeatedly renewed,
To remove unreacted metal cation, the P(MVE-alt-MA of the different degrees of cross linking is obtained)/M+Alg hydrogel, M+For metal sun
Ion.
3. the preparation method of hydrogel according to claim 2, it is characterised in that the poly- (methyl vinyl ether-alt-
Maleic anhydride) number-average molecular weight be 311000, PEG number-average molecular weight be 22000.
4. the purposes that hydrogel described in claim 1 carries out cell culture as 3D culture timbering material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610133596.1A CN105771004B (en) | 2016-03-09 | 2016-03-09 | A kind of hydrogel, preparation method and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610133596.1A CN105771004B (en) | 2016-03-09 | 2016-03-09 | A kind of hydrogel, preparation method and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105771004A CN105771004A (en) | 2016-07-20 |
| CN105771004B true CN105771004B (en) | 2019-07-12 |
Family
ID=56387990
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610133596.1A Active CN105771004B (en) | 2016-03-09 | 2016-03-09 | A kind of hydrogel, preparation method and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105771004B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107082899B (en) * | 2017-04-18 | 2020-04-28 | 深圳大学 | Method for improving rigidity and self-healing property of tough hydrogel |
| CN109810935B (en) * | 2017-11-20 | 2022-12-13 | 中国科学院大连化学物理研究所 | Preparation method of gelatin methacrylamide core-shell microspheres for cell partition culture |
| CN110862562B (en) * | 2019-11-28 | 2021-08-31 | 西南交通大学 | 3D cell culture medium and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2384745A2 (en) * | 2010-05-05 | 2011-11-09 | Sanovel Ilac Sanayi ve Ticaret A.S. | Modified Release Pharmaceutical Compositions Of Dexlansoprazole |
| CN102952279A (en) * | 2012-05-10 | 2013-03-06 | 东南大学 | Hydrogel for three-dimensional culturing of tumor cells and applications thereof |
| CN104271168A (en) * | 2012-03-06 | 2015-01-07 | Uab研究基金会 | Three-dimensional, prevascularized, engineered tissue constructs, methods of making and methods of using the tissue constructs |
| CN104262667A (en) * | 2014-09-18 | 2015-01-07 | 哈尔滨工业大学 | Alginate-based material external controllable ionic crosslinked method |
-
2016
- 2016-03-09 CN CN201610133596.1A patent/CN105771004B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2384745A2 (en) * | 2010-05-05 | 2011-11-09 | Sanovel Ilac Sanayi ve Ticaret A.S. | Modified Release Pharmaceutical Compositions Of Dexlansoprazole |
| CN104271168A (en) * | 2012-03-06 | 2015-01-07 | Uab研究基金会 | Three-dimensional, prevascularized, engineered tissue constructs, methods of making and methods of using the tissue constructs |
| CN102952279A (en) * | 2012-05-10 | 2013-03-06 | 东南大学 | Hydrogel for three-dimensional culturing of tumor cells and applications thereof |
| CN104262667A (en) * | 2014-09-18 | 2015-01-07 | 哈尔滨工业大学 | Alginate-based material external controllable ionic crosslinked method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105771004A (en) | 2016-07-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Qureshi et al. | Polysaccharide based superabsorbent hydrogels and their methods of synthesis: A review | |
| Feng et al. | Synthesis and swelling behaviors of yeast-g-poly (acrylic acid) superabsorbent co-polymer | |
| CN102688525B (en) | Bio-macromolecular hydrogel and preparation method thereof | |
| CN104140630B (en) | A kind of chitosan-based double-network hydrogel and preparation method thereof | |
| CN106243410B (en) | A kind of hydroxyethyl chitosan/hyaluronic acid double-network hydrogel and preparation method thereof | |
| CN105713106B (en) | Double cross-linked hydrogels of a kind of sodium alginate and preparation method and application | |
| CN105771004B (en) | A kind of hydrogel, preparation method and its application | |
| CN106866998B (en) | A kind of chitosan quaternary ammonium salt/carboxymethyl cellulose superabsorbent hydrogel and its preparation method and application | |
| CN108371728A (en) | A kind of imitative mussel for tissue repair contacts the preparation method of anti-bacterial hydrogel | |
| CN103948962B (en) | Method for preparing growth-factor bound thermo-sensitive hydrogel biocarrier | |
| Nwe et al. | Selection of a biopolymer based on attachment, morphology and proliferation of fibroblast NIH/3T3 cells for the development of a biodegradable tissue regeneration template: Alginate, bacterial cellulose and gelatin | |
| CA2817229A1 (en) | Gels and hydrogels | |
| CN105288704A (en) | Polyamide-amine dendrimer modified chitosan wound repair hydrogel as well as preparation method and application thereof | |
| CN106693050A (en) | Preparation method for composite scaffold material based on collagen and collagen fibers | |
| Yuan et al. | Cationic peptide-based salt-responsive antibacterial hydrogel dressings for wound healing | |
| CN107812540B (en) | Non-recovery natural polysaccharide hydrogel-based photocatalyst pre-radiation synthesis method | |
| CN112341640A (en) | Bio-based self-repairing hydrogel and preparation method and application thereof | |
| CN104845382A (en) | Silk protein/cellulose derivative blending hydrogel and preparation method thereof | |
| CN112062981A (en) | Preparation method of culture medium mediated crosslinked hyaluronic acid-based double-crosslinked hydrogel | |
| CN109096501A (en) | A kind of fibroin three-dimensional porous rack and preparation method thereof | |
| CN106146912A (en) | A kind of calcium alginate hydrogel with three-dimensional micro-pattern and preparation method thereof | |
| CN106084303A (en) | A kind of Biodegradable high-molecular is composite porous and preparation method thereof | |
| CN103007342B (en) | Biodegradable and medical tricalcium phosphate/gamma-polyglutamic acid composite and preparation method thereof | |
| Miao et al. | Engineered bone tissues using biomineralized gelatin methacryloyl/sodium alginate hydrogels | |
| CN102757625B (en) | A kind of chitosan-polylactic acid is composite porous and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |