CN105758912A - Preparation and application of nano TiO2-MoS2 photoelectric Saos-2 cell sensor - Google Patents

Preparation and application of nano TiO2-MoS2 photoelectric Saos-2 cell sensor Download PDF

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CN105758912A
CN105758912A CN201610245278.4A CN201610245278A CN105758912A CN 105758912 A CN105758912 A CN 105758912A CN 201610245278 A CN201610245278 A CN 201610245278A CN 105758912 A CN105758912 A CN 105758912A
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cell
saos
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CN105758912B (en
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庞雪辉
马洪敏
胡丽华
张勇
吴丹
范大伟
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University of Jinan
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
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    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/305—Electrodes, e.g. test electrodes; Half-cells optically transparent or photoresponsive electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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Abstract

The invention relates to preparation and application of a nano TiO2-MoS2 photoelectric Saos-2 cell sensor and belongs to the technical field of biological sensing and detecting.A Ti O2-MoS2 compound is used as a beacon substance, qualitative and quantitative detection of solid tissue target Saos-2 cells can be realized, equipment is simple, cost is low, and the sensor is easy to miniaturize.

Description

A kind of nano-TiO2-MoS2The preparation of photoelectricity Saos-2 cell cell sensor and application thereof
Technical field
The present invention relates to a kind of nano-TiO2-MoS2The preparation of photoelectricity Saos-2 cell cell sensor and application, the biological tissue Saos-2 that this sensor is used for The detection of cell cell, belongs to bio-sensing detection technique field.
Background technology
Enterprise participates in scientific and technical innovation, unites with scientific and technological circle, it is achieved the conversion of scientific and technological achievement, and the common development for national economy is contributed, and has become as the common recognition of the whole society.Checking and improve laboratory scientific achievement, and be the in-depth research of China's life sciences, gather mass data storehouse and big colony Sample Storehouse, build new technology platform and support system.In consideration of it, this patent will utilize TiO2-MoS2The signal amplification of nano composite material, utilize the Strength Changes of light electrical signal in system that true biological tissue Saos-2 cell cell of extracting after cultivating is detected, and during the photoelectricity biosensor further genralrlization developed is applied to reality, the method has the advantages such as low cost, highly sensitive, specificity good, detection is quick, and preparation process is relatively simple, largely overcome current Saos-2 Cell cell detection method is confined to the drawback of pure biological field, has effectively expanded the scope of Saos-2 cell cell detection method.
Summary of the invention
An object of the present invention is the biospecific affinity to Saos-2 cell cell and nano photoelectric composite TiO2-MoS2, it is prepared for one and possesses specificity, overdelicate photoelectricity biosensor；
The two of the purpose of the present invention are the detections of the Saos-2 cell cell that this sensor is used for solid tissue extracting.
Technical scheme is as follows:
1. a nano-TiO2-MoS2The preparation of photoelectricity Saos-2 cell cell sensor
(1) by 1.0 The rectangle ITO electro-conductive glass of cm × 2.5 cm, cleans 20 min with acetone, ultra-pure water, EtOH Sonicate successively, and purity nitrogen dries up, and as working electrode, platinized platinum is to electrode, and saturated calomel electrode is reference electrode, constitutes three-electrode cell；
(2) on ITO electrode surface, drop coating 5 ~ 20 L TiO2Nano wire；
TiO2The preparation of nano wire
15 ~ 25 mL CTAB solution join in 20 ~ 30 mL TTIP solution, stirring 0.5 ~ 1.5 h, add 50 ~ 60 mL ethylene glycol and 0.6 ~ 1.2 g carbamide, stir 1 h, it is then transferred in 100 mL autoclaves, heats 15 ~ 25 h at 140 ~ 160 DEG C, collect solid, with distilled water wash 3 ~ 8 times, put into and air dry oven heats at 75 ~ 85 DEG C 12 ~ 36 H, prepares TiO2Nano wire；
TTIP solution is to be joined in 12 ~ 14 g concentrated hydrochloric acid by 0.16 ~ 0.40 g TTIP, is stirred vigorously, and prepares；
CTAB solution is to join in 25 ~ 30 mL distilled water by 0.15 ~ 0.25 g CTAB, stirs 20 ~ 40 min, prepares；
(3) by 0.5 ~ 1.5 g MoS2Quantum dot is coated onto TiO2In nanowire surface；
MoS2The preparation of quantum dot
20 ~ 30 mL sodium molybdate solutions and the mixing of 15 ~ 25 mL Cys solution, ultrasonic 10 ~ 20 min, put into and heat 24 ~ 48 h in autoclave at 200 DEG C, and distilled water wash 3 ~ 8 times is centrifugal under 8000 rpm/min, takes precipitation, prepares MoS2Quantum dot；
Sodium molybdate solution is that 0.2 ~ 0.3 g sodium molybdate is joined 20 ~ 30 In mL distilled water, ultrasonic 5 ~ 15 min, add 1mol/L salt acid for adjusting pH to 6.5, prepare；
Cys solution is to join in 50 mL distilled water by 0.3 ~ 0.8 g Cys, prepares；
(4) drop coating 10 ~ 20 is continued L, target Saos-2 of 5 ~ 50 g/mL The specific antibody of cell cell, to working electrode surface, is dried in 4 DEG C of refrigerators, prepares a kind of nano-TiO2-MoS2Photoelectricity Saos-2 cell cell sensor.
2. the detecting step of target Saos-2 cell cell
(1) using electrochemical workstation to test with three-electrode system, saturated calomel electrode is reference electrode, and platinum plate electrode is auxiliary electrode, prepared TiO2-MoS2Photoelectricity Saos-2 cell cell sensor is working electrode, 10 ~ 50 The ascorbic acid buffer solution of the PBS of mL, pH 7 ~ 9,0.1 ~ 0.3 mmol/L is tested；
(2) target Saos-2 cell cell standard solution is detected by used time m-current method, and input voltage is 0.1 V, sampling interval 20 s, and sample time 20, s, ran time 500 s, and light source selects 430 nm, and record current changes, drawing curve；
(3) testing sample is replaced target Saos-2 Cell cell standard solution, is measured according to the method for drafting of working curve.
The achievement of the present invention:
(1) the method is not required to through pretreatment, and electrode selectivity, sensitivity and repeatability are preferable, and electrode response is fast, records the range of linearity 2 ~ 10000 cell/mL, detection is limited to 2 cell/mL.
(2) present invention uses TiO2-MoS2As photoelectricity beacon material, MoS2Quantum dot is to TiO2Doping substantially reduce distance therebetween, promote TiO2Photoelectric properties, strengthen the photoelectric properties of sensor of the present invention.
(3) present invention detected Saos-2 cell cell all extracts from actual biological tissue, has certain practical value.
Detailed description of the invention:
For further illustrating, illustrate in conjunction with embodiment once:
Embodiment 1A kind of nano-TiO2-MoS2The preparation of photoelectricity Saos-2 cell cell sensor
(1) by 1.0 The rectangle ITO electro-conductive glass of cm × 2.5 cm, cleans 20 min with acetone, ultra-pure water, EtOH Sonicate successively, and purity nitrogen dries up, and as working electrode, platinized platinum is to electrode, and saturated calomel electrode is reference electrode, constitutes three-electrode cell；
(2) on ITO electrode surface, drop coating 5 L TiO2Nano wire；
(3) by 0.5 g MoS2Quantum dot is coated onto TiO2In nanowire surface；
(4) specific antibody of the target Saos-2 cell cell of continuation drop coating 10 L, 5 g/mL is to working electrode surface, dries in 4 DEG C of refrigerators, prepares a kind of nano-TiO2-MoS2Photoelectricity Saos-2 cell cell sensor.
Embodiment 2A kind of nano-TiO2-MoS2The preparation of photoelectricity Saos-2 cell cell sensor
(1) by 1.0 The rectangle ITO electro-conductive glass of cm × 2.5 cm, cleans 20 min with acetone, ultra-pure water, EtOH Sonicate successively, and purity nitrogen dries up, and as working electrode, platinized platinum is to electrode, and saturated calomel electrode is reference electrode, constitutes three-electrode cell；
(2) on ITO electrode surface, drop coating 15 L TiO2Nano wire；
(3) by 1 g MoS2Quantum dot is coated onto TiO2In nanowire surface；
(4) specific antibody of the target Saos-2 cell cell of continuation drop coating 15 L, 25 g/mL is to working electrode surface, dries in 4 DEG C of refrigerators, prepares a kind of nano-TiO2-MoS2Photoelectricity Saos-2 cell cell sensor.
Embodiment 3A kind of nano-TiO2-MoS2The preparation of photoelectricity Saos-2 cell cell sensor
(1) by 1.0 The rectangle ITO electro-conductive glass of cm × 2.5 cm, cleans 20 min with acetone, ultra-pure water, EtOH Sonicate successively, and purity nitrogen dries up, and as working electrode, platinized platinum is to electrode, and saturated calomel electrode is reference electrode, constitutes three-electrode cell；
(2) on ITO electrode surface, drop coating 5 ~ 20 L TiO2Nano wire；
(3) by 1.5 g MoS2Quantum dot is coated onto TiO2In nanowire surface；
(4) specific antibody of the target Saos-2 cell cell of continuation drop coating 20 L, 50 g/mL is to working electrode surface, dries in 4 DEG C of refrigerators, prepares a kind of nano-TiO2-MoS2Photoelectricity Saos-2 cell cell sensor.
Embodiment 4 TiO2The preparation of nano wire
15 mL CTAB solution join in 30 mL TTIP solution, stir 1.5 h, add 50 mL ethylene glycol and 1.2 g carbamide, stir 1 h, be then transferred in 100 mL autoclaves, at 150 DEG C, heat 18 h, collect solid, with distilled water wash 5 times, put into and air dry oven heats at 80 DEG C 24 h, prepare TiO2Nano wire；
TTIP solution is to be joined in 12 g concentrated hydrochloric acid by 0.24 g TTIP, is stirred vigorously, and prepares；
CTAB solution is to be joined in 30 mL distilled water by 0.15 g CTAB, stirs 25 min, prepares.
Embodiment 5 MoS2The preparation of quantum dot
20 mL sodium molybdate solutions and the mixing of 25 mL Cys solution, ultrasonic 15 Min, puts into and heats 36 h in autoclave at 200 DEG C, distilled water wash 5 times, centrifugal under 8000 rpm/min, takes precipitation, prepares MoS2Quantum dot；
Sodium molybdate solution is to join in 30 mL distilled water by 0.2 g sodium molybdate, ultrasonic 10 min, adds 1mol/L salt acid for adjusting pH to 6.5, prepares；
Cys solution is to join in 50 mL distilled water by 0.6 g Cys, prepares.
Embodiment 6 The detection of Saos-2 cell cell
(1) using electrochemical workstation to test with three-electrode system, saturated calomel electrode is reference electrode, and platinum plate electrode is auxiliary electrode, prepared TiO2-MoS2Photoelectricity Saos-2 cell cell sensor is working electrode, tests in the PBS of 50 mL, pH 7.4, the ascorbic acid buffer solution of 0.1 mmol/L；
(2) target Saos-2 cell cell standard solution is detected by used time m-current method, and input voltage is 0.1 V, sampling interval 20 s, and sample time 20, s, ran time 500 s, and light source selects 430 nm, and record current changes, drawing curve；
(3) testing sample is replaced target Saos-2 Cell cell standard solution, is measured according to the method for drafting of working curve；
(4) range of linearity 2 ~ 10000 cell/mL, detection is limited to 2cell/mL.

Claims (2)

1. a nano-TiO2-MoS2The preparation method of photoelectricity Saos-2 cell cell sensor, it is characterised in that comprise the following steps:
(1) by the rectangle ITO electro-conductive glass of 1.0 cm × 2.5 cm, cleaning 20 min with acetone, ultra-pure water, EtOH Sonicate successively, purity nitrogen dries up, and as working electrode, platinized platinum is to electrode, and saturated calomel electrode is reference electrode, constitutes three-electrode cell；
(2) on ITO electrode surface, drop coating 5 ~ 20 L TiO2Nano wire；
Described TiO2Nano wire, preparation process is as follows:
15 ~ 25 mL CTAB solution join 20 ~ 30 mL In TTIP solution, stir 0.5 ~ 1.5 h, add 50 ~ 60 mL ethylene glycol and 0.6 ~ 1.2 g carbamide, stir 1 h, be then transferred in 100 mL autoclaves, at 140 ~ 160 DEG C, heat 15 ~ 25 h, collect solid, with distilled water wash 3 ~ 8 times, put in air dry oven 75 ~ 85 Heat 12 ~ 36 h at DEG C, prepare TiO2Nano wire；
Described TTIP solution is to be joined in 12 ~ 14 g concentrated hydrochloric acid by 0.16 ~ 0.40 g TTIP, is stirred vigorously, and prepares；
Described CTAB solution is to join in 25 ~ 30 mL distilled water by 0.15 ~ 0.25 g CTAB, stirs 20 ~ 40 min, prepares；
(3) by 0.5 ~ 1.5 g MoS2Quantum dot is coated onto TiO2In nanowire surface；
Described MoS2Quantum dot, preparation process is as follows:
20 ~ 30 mL sodium molybdate solutions and 15 ~ 25 mL Cys solution mixes, and ultrasonic 10 ~ 20 Min, puts into and heats 24 ~ 48 h in autoclave at 200 DEG C, and distilled water wash 3 ~ 8 times is centrifugal under 8000 rpm/min, takes precipitation, prepares MoS2Quantum dot；
Described sodium molybdate solution is by 0.2 ~ 0.3 G sodium molybdate joins in 20 ~ 30 mL distilled water, ultrasonic 5 ~ 15 min, adds 1mol/L salt acid for adjusting pH to 6.5, prepares；
Described Cys solution is to join in 50 mL distilled water by 0.3 ~ 0.8 g Cys, prepares；
(4) specific antibody of the target Saos-2 cell cell of continuation drop coating 10 ~ 20 L, 5 ~ 50 g/mL is to working electrode surface, dries in 4 DEG C of refrigerators, prepares a kind of nano-TiO2-MoS2Photoelectricity Saos-2 cell cell sensor.
2. preparation method as claimed in claim 1 prepares nano-TiO2-MoS2Photoelectricity Saos-2 cell cell sensor, for the detection of target Saos-2 cell cell, detecting step is as follows:
(1) using electrochemical workstation to test with three-electrode system, saturated calomel electrode is reference electrode, and platinum plate electrode is auxiliary electrode, prepared TiO2-MoS2Photoelectricity Saos-2 cell cell sensor is working electrode, tests in the PBS of 10 ~ 50 mL, pH 7 ~ 9, the ascorbic acid buffer solution of 0.1 ~ 0.3 mmol/L；
(2) target Saos-2 cell cell standard solution is detected by used time m-current method, and input voltage is 0.1 V, sampling interval 20 s, and sample time 20, s, ran time 500 s, and light source selects 430 nm, and record current changes, drawing curve；
(3) testing sample is replaced target Saos-2 cell cell standard solution, is measured according to the method for drafting of working curve.
CN201610245278.4A 2016-04-20 2016-04-20 A kind of nano-TiO2‑MoS2The application of the cell cell sensors of photoelectricity Saos 2 Expired - Fee Related CN105758912B (en)

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CN113848243A (en) * 2021-09-23 2021-12-28 西南大学 Electrochemiluminescence material without exogenous luminophor and its prepn and application
CN115323429A (en) * 2022-09-05 2022-11-11 深圳先进技术研究院 Preparation method of quantum dot sensitized composite photo-anode, quantum dot sensitized composite photo-anode and application

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CN106290875A (en) * 2016-08-10 2017-01-04 河南大学 TiO with triple modifications2nano-wire array is the construction method of the electrochemical immunosensor of support
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CN113848243A (en) * 2021-09-23 2021-12-28 西南大学 Electrochemiluminescence material without exogenous luminophor and its prepn and application
CN113848243B (en) * 2021-09-23 2024-01-30 西南大学 Preparation method and application of electrochemiluminescence material without exogenous luminophor
CN115323429A (en) * 2022-09-05 2022-11-11 深圳先进技术研究院 Preparation method of quantum dot sensitized composite photo-anode, quantum dot sensitized composite photo-anode and application

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