CN105758689A - Bacteria grinding dispersion device and bacteria block grinding method - Google Patents
Bacteria grinding dispersion device and bacteria block grinding method Download PDFInfo
- Publication number
- CN105758689A CN105758689A CN201610247940.XA CN201610247940A CN105758689A CN 105758689 A CN105758689 A CN 105758689A CN 201610247940 A CN201610247940 A CN 201610247940A CN 105758689 A CN105758689 A CN 105758689A
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- Prior art keywords
- magnetically
- bacteria
- grinding
- actuated
- mill bacterium
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Finish Polishing, Edge Sharpening, And Grinding By Specific Grinding Devices (AREA)
- Crushing And Grinding (AREA)
Abstract
The invention discloses a bacteria grinding dispersion device and a bacteria block grinding method.The bacteria grinding dispersion device comprises a bacteria grinding container, a plurality of magnetic driving parts are arranged around the bacteria grinding container and surround to form a magnetic driving cavity, the bacteria grinding container is positioned in the magnetic driving cavity, and grinding beads which can be driven magnetically are arranged in the bacteria grinding container.A mode of manual bacteria grinding is simulated by means of magnetically driving the grinding beads to grind bacteria blocks, so that compared with manual bacteria grinding, the bacteria block grinding method has the advantages of higher efficiency and higher uniformity in grinding; compared with a mode of ultrasonic dispersion, the bacteria block grinding method has the advantages that no damage is caused to ground bacteria blocks, and biological performances of the ground bacteria blocks are not damaged.The bacteria grinding dispersion device and the bacteria block grinding method can be used for bacteria block grinding.
Description
Technical field
The present invention relates to antibacterial field of dispersions, particularly relate to a kind of mill bacterium dispersal device and truffle Ginding process.
Background technology
Controlling key one step that bacterial concentration is test bacteria bio performance, bacterial concentration is too high or too low, will affect test result, and it therefore must be made to drop in the scope of prescribed by standard.Generally take two kinds of methods to measure bacterial concentration at present: one is viable bacteria counting method, this method can judge bacterial concentration exactly, but there is obvious hysteresis quality, its actual concentrations cannot be determined within the very first time, until result out after, starting detection again, the activity of bacterium will decline to some extent, bring inconvenience to laboratory operation;Two is turbidimetry, and this method can judge bacterial concentration rapidly, has simplicity, rapidly, it is possible to the advantage of METHOD FOR CONTINUOUS DETERMINATION, is suitable for automatically controlling.
Turbidimetry is the quantity that the light transmission capacity according to bacteria suspension measures antibacterial indirectly, therefore, how to be fast and effeciently dispersed in dispersion liquid by truffle, is the committed step of turbidimetry.Method conventional at present has manual method and ultrasonic method.Wherein, manual method is to utilize the means such as hand-ground dispersion, liquid-transfering gun piping and druming dispersion, vortex concussion dispersion, truffle is disperseed in the solution, its shortcoming is weak effect, speed is slow, heterogeneity, and be all greatly improved space to bacterium solution sealing, rate of dispersion and dispersion effect, and without unified operation or work standard, the subjectivity by operator judges completely;Ultrasonic method is to substitute the mechanical lapping such as hand-ground, vortex concussion with ultrasound wave dispersion, the advantage with hermetically sealed (safety), efficiently (high speed and high-quality), standard operation (SOP), its shortcoming is that hyperacoustic cavitation can produce substantial amounts of high pressure-temperature bubble, localized hyperthermia when breaking, the cell wall of destructible antibacterial, thus causing bacterial death, affect follow-up experimental result.
Summary of the invention
In order to solve the problems referred to above, it is an object of the invention to provide a kind of mill bacterium dispersal device, keep every biological property of truffle.
The technical solution adopted in the present invention is:
A kind of mill bacterium dispersal device, including mill bacterium container, described mill bacterium container peripheral ring is arranged with some magnetically-actuated parts, surrounds magnetically-actuated chamber between each magnetically-actuated parts, described mill bacterium container is positioned at magnetically-actuated intracavity, and being provided with in described mill bacterium container can by multiple grinding bead of magnetically-actuated.
As a further improvement on the present invention, the top and bottom of described mill bacterium container is equipped with magnetically-actuated parts.
As a further improvement on the present invention, also include a building enclosure, described building enclosure includes circumference and goes along with sb. to guard him and connect circumference and go along with sb. to guard him the top cover on top, connect circumference and go along with sb. to guard him the bottom of bottom, described magnetically-actuated parts be separately positioned on circumference go along with sb. to guard him, on top cover and bottom, thus forming described magnetically-actuated chamber in building enclosure.
As a further improvement on the present invention, described building enclosure is polygonal column body, and described magnetically-actuated parts are arranged on each of polygonal column body.
As a further improvement on the present invention, also including controlling parts, each described magnetically-actuated parts are all electrically connected on control parts.
As a further improvement on the present invention, described grinding bead includes the iron shot of internal layer and the wear resistant outer layer of parcel covering iron shot.
As a further improvement on the present invention, the material of described wear resistant outer layer is glass or politef.
Another object of the present invention is to provide a kind of truffle Ginding process, it the technical scheme is that
Utilize the alternating magnetic field that magnetically-actuated parts produce to drive each grinding bead in mill bacterium container at magnetically-actuated intracavity, and produced mill bacterium effect by the relative motion between grinding bead and phase mutual friction.
On the basis of above-mentioned truffle Ginding process, control the start and stop of each magnetically-actuated parts respectively to adjust the movement locus of grinding bead.
On the basis of above-mentioned truffle Ginding process, mill bacterium container automatic liquid matching and automatic ratio is turbid.
The invention has the beneficial effects as follows: the mode that the present invention grinds truffle by magnetically-actuated grinding bead imitates the manual mode grinding bacterium, in hgher efficiency compared to manual mill bacterium, grind more homogeneous, relative to the scattered mode of ultrasound wave, to ground truffle fanout free region, every biological property of ground truffle will not be destroyed.
Accompanying drawing explanation
Below in conjunction with drawings and embodiments, the present invention is further described.
Fig. 1 is the structural representation of dispersal device.
Detailed description of the invention
Mill bacterium dispersal device in embodiment, as it is shown in figure 1, at least include a mill bacterium container 1 that can be sealed, is used for containing dispersion liquid and truffle.At least it is arranged with several and not shown magnetically-actuated parts at mill bacterium container 1 peripheral ring, these magnetically-actuated parts produce electromagnetic induction phenomenon after powered up and produce the magnetic field of alternation respectively, thus defining a magnetically-actuated chamber 2 between each magnetically-actuated parts, described mill bacterium container 1 is positioned at magnetically-actuated chamber 2.It addition, be additionally provided with in mill bacterium container 1 can by multiple grinding bead 3 of magnetically-actuated, under the magneticaction of alternating magnetic field, these grinding bead 3 in dispersion liquid can relative motion, and collide each other and rub.
Preferably, not only magnetically-actuated parts are set in the periphery of mill bacterium container 1, top and bottom at mill bacterium container 1 is equipped with magnetically-actuated parts, more magnetically-actuated parts can so that grinding bead 3 has more rich movement locus, and the more movement locus of grinding bead 3 can produce better grinding effect.
Magnetically-actuated chamber 2 can be formed in the following manner.
Being provided with a building enclosure outside mill bacterium container 1, building enclosure includes circumference and goes along with sb. to guard him 41 and connect circumference and go along with sb. to guard him the top cover 42 on 41 tops, connect circumference and go along with sb. to guard him the bottom 43 of 41 bottoms.Top cover 42 correspondence is at the top of mill bacterium container 1, and bottom 43 correspondence is bottom mill bacterium container 1, and circumference is gone along with sb. to guard him 41 correspondences and is looped around the sidewall of mill bacterium container 1.Magnetically-actuated parts be separately positioned on circumference go along with sb. to guard him 41, on top cover 42 and bottom 43, thus forming above-mentioned magnetically-actuated chamber 2 in building enclosure.
In general, circumference goes along with sb. to guard him 41, top cover 42, bottom 43 at least one can open, to facilitate putting into of mill bacterium container 1.
In embodiment, bottom 43 upper surface is provided with and not shown draw-in groove, in order to connect and fix described mill bacterium container 1.
Preferably, building enclosure is polygonal column body, such as the regular hexagon column of Fig. 1, and described magnetically-actuated parts are arranged on each of polygonal column body.
Preferably, mill bacterium dispersal device also includes and not shown control parts, it is positioned at the outside of building enclosure, or it is directly installed in building enclosure, and each magnetically-actuated parts are all electrically connected on control parts, controlling the start and stop of different magnetically-actuated parts by controlling parts, so that producing different magnetic force direction in mill bacterium container 1, changing the movement locus of grinding bead 3.
Preferably, grinding bead 3 includes the iron shot of internal layer and the wear resistant outer layer of parcel covering iron shot.The material of above-mentioned wear resistant outer layer can be glass or politef, is generally non-magnetic material.
In general grinding bead 3 diameter is 0.8 ~ 1.2mm, and iron shot diameter is 0.6 ~ 0.8mm, and wear resistant outer layer diameter is 0.2 ~ 0.4mm, and 20 ~ 50 grinding bead 3 need to be put into during use mill bacterium container 1.
Use the method that above-mentioned mill bacterium dispersal device carries out antibacterial grinding as follows:
First, dispersion liquid, truffle and grinding bead 3 are loaded in mill bacterium container 1, then, mill bacterium container 1 is put into magnetically-actuated chamber 2, connect the power supply of magnetically-actuated parts so that it is produce the magnetic field of alternation, in order to drive each grinding bead 3 in mill bacterium container 1 to produce irregular motion, or the changing straight line in direction and/or rotary motion, produce mill bacterium effect by the relative motion between grinding bead 3 and phase mutual friction;After antibacterial truffle is milled to uniform turbidity, mill bacterium solution is taken out, detects turbidity in real time.
On the basis of above-mentioned truffle Ginding process, by controlling parts and controlling the start and stop of each magnetically-actuated parts in the program controlling to preset on parts respectively, thus adjusting the movement locus of grinding bead.
Moreover, user can be that mill bacterium dispersal device is equipped with automatic liquid matching and automatic turbid installations and facilities of ratio, in the way of substituting and being manually placed into, take out mill bacterium container, comes mill bacterium container automatic liquid matching and ratio is turbid automatically so that operation more simplifies, in hgher efficiency.
The above is the preferred embodiment of the present invention, and it is not intended that limiting the scope of the invention.
Claims (10)
1. a mill bacterium dispersal device, including mill bacterium container (1), it is characterized in that: described mill bacterium container (1) peripheral ring is arranged with some magnetically-actuated parts, magnetically-actuated chamber (2) is surrounded between each magnetically-actuated parts, described mill bacterium container (1) is positioned at magnetically-actuated chamber (2), and being provided with in described mill bacterium container (1) can by multiple grinding bead (3) of magnetically-actuated.
2. mill bacterium dispersal device according to claim 1, it is characterised in that: the top and bottom of described mill bacterium container (1) is equipped with magnetically-actuated parts.
3. mill bacterium dispersal device according to claim 2, it is characterized in that: also include a building enclosure, described building enclosure includes circumference and goes along with sb. to guard him (41) and connect circumference and go along with sb. to guard him the top cover (42) on (41) top, connect circumference and go along with sb. to guard him the bottom (43) of (41) bottom, described magnetically-actuated parts are separately positioned on circumference and go along with sb. to guard him on (41), top cover (42) and bottom (43), thus forming described magnetically-actuated chamber (2) in building enclosure.
4. mill bacterium dispersal device according to claim 3, it is characterised in that: described building enclosure is polygonal column body, and described magnetically-actuated parts are arranged on each of polygonal column body.
5. the mill bacterium dispersal device according to claim 1 or 2 or 3, it is characterised in that: also including controlling parts, each described magnetically-actuated parts are all electrically connected on control parts.
6. the mill bacterium dispersal device according to claim 1 or 2 or 3, it is characterised in that: described grinding bead (3) includes the iron shot of internal layer and the wear resistant outer layer of parcel covering iron shot.
7. mill bacterium dispersal device according to claim 6, it is characterised in that: the material of described wear resistant outer layer is glass or politef.
8. a truffle Ginding process, it is characterized in that: the alternating magnetic field utilizing magnetically-actuated parts to produce in magnetically-actuated chamber (2) drives each grinding bead (3) in mill bacterium container (1), and is produced mill bacterium effect by the relative motion between grinding bead (3) and phase mutual friction.
9. truffle Ginding process according to claim 8, it is characterised in that: control the start and stop of each magnetically-actuated parts respectively to adjust the movement locus of grinding bead (3).
10. truffle Ginding process according to claim 8 or claim 9, it is characterised in that: mill bacterium container (1) automatic liquid matching and automatic ratio is turbid.
Priority Applications (1)
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CN201610247940.XA CN105758689A (en) | 2016-04-20 | 2016-04-20 | Bacteria grinding dispersion device and bacteria block grinding method |
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CN201610247940.XA CN105758689A (en) | 2016-04-20 | 2016-04-20 | Bacteria grinding dispersion device and bacteria block grinding method |
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6814319B2 (en) * | 2000-12-06 | 2004-11-09 | Pharmacia & Upjohn Company | Laboratory scale milling process |
JP4903419B2 (en) * | 2005-04-20 | 2012-03-28 | 安井器械株式会社 | Crushing method, crushing apparatus and crushing processing apparatus using the same |
WO2012046089A1 (en) * | 2010-10-04 | 2012-04-12 | Dna-Technology Jsc | Magnetic tissue grinding |
CN204630792U (en) * | 2015-05-20 | 2015-09-09 | 荣成宏秀山磁业有限公司 | A kind of metallographic grind away frock of neodymium-iron-boron post |
CN106102898A (en) * | 2014-03-14 | 2016-11-09 | 株式会社岛津制作所 | The operational approach of magnetic granule and magnetic particle manipulation device |
CN205941115U (en) * | 2016-04-20 | 2017-02-08 | 广东南芯医疗科技有限公司 | Grind fungus dispersion devices |
-
2016
- 2016-04-20 CN CN201610247940.XA patent/CN105758689A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6814319B2 (en) * | 2000-12-06 | 2004-11-09 | Pharmacia & Upjohn Company | Laboratory scale milling process |
JP4903419B2 (en) * | 2005-04-20 | 2012-03-28 | 安井器械株式会社 | Crushing method, crushing apparatus and crushing processing apparatus using the same |
WO2012046089A1 (en) * | 2010-10-04 | 2012-04-12 | Dna-Technology Jsc | Magnetic tissue grinding |
CN106102898A (en) * | 2014-03-14 | 2016-11-09 | 株式会社岛津制作所 | The operational approach of magnetic granule and magnetic particle manipulation device |
CN204630792U (en) * | 2015-05-20 | 2015-09-09 | 荣成宏秀山磁业有限公司 | A kind of metallographic grind away frock of neodymium-iron-boron post |
CN205941115U (en) * | 2016-04-20 | 2017-02-08 | 广东南芯医疗科技有限公司 | Grind fungus dispersion devices |
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Application publication date: 20160713 |
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