CN105748954A

CN105748954A - Preparation method of traditional Chinese medicine composition with functions of treating insomnia, improving senile dementia, enhancing learning and memory abilities, resisting to depression and anxiety and soothing the nerves and benefitting intelligence

Info

Publication number: CN105748954A
Application number: CN201610134125.2A
Authority: CN
Inventors: 王林元; 张建军
Original assignee: BEIJING CAIRUI MEDICAL TECHNOLOGY Co Ltd
Current assignee: BEIJING CAIRUI MEDICAL TECHNOLOGY Co Ltd
Priority date: 2016-03-09
Filing date: 2016-03-09
Publication date: 2016-07-13

Abstract

The invention discloses a preparation method of a traditional Chinese medicine composition with functions of treating insomnia, improving senile dementia, enhancing learning and memory abilities, resisting to depression and anxiety and soothing the nerves and benefitting intelligence.The traditional Chinese medicine composition comprises Rhizoma Gastrodiae, Radix Paeoniae Alba, Radix Acanthopanacis Senticosi and Semen Ziziphi Spinosae.The traditional Chinese medicine composition prepared with the method can be prepared into dosage forms acceptable clinically or pharmaceutically.Animal experiments show that the traditional Chinese medicine composition prepared with the method can improve the sleeping function and senile dementia and enhance learning and memory abilities, and has the anti-depression and anti-anxiety effects.

Description

A kind of have treatment insomnia, the preparation method improving the Chinese medicine composition of senile dementia, raising learning and memory, resolving depression effect

Technical field

The invention belongs to Chinese medicinal composition preparation field, be specifically related to a kind of preparation method for treating insomnia, improve senile dementia, improve the Chinese medicine composition of ability of learning and memory, antidepressant, anxiety, nootropic effect of calming the nerves.

Background technology

The traditional Chinese medical science thinks that eating and drinking without temperance, daily life are variable, feelings will is unsuccessful, work and rest is immoderate, diseases caused by exogenous pathogenic factor six climate exopathogens heresy poison (such as environmental pollution) etc. all can cause internal organs negative and positive of qi and blood to lack of proper care, and ultimately results in the generation that sleep state is not good.Therefore, the crowd of, malaise partially high for deficiency of heart-QI, liver-yang, the health-care method adopting suppressing liver-YANG, QI invigorating to allay excitement, can fundamentally improve sleep.

Senile dementia includes vascular and two kinds of histological type of Alzheimer.Senile dementia is a kind of able-bodied chronic neurodegenerative disease of serious harm elderly population, and its first symptoms is intelligence, memory, sense organ capacity of orientation, the irreversible Progressive symmetric erythrokeratodermia degeneration of relation between Thinking, Language ability, generally entail that personality change.Having data to show, the old people of 5-15% has Alzheimer disease symptoms in various degree.Senile dementia characteristic pathological is changed to atrophy of cerebral cortex, and deposits with amyloid-beta, neurofibrillary tangles, and mass memory neuron number reduces, and the formation of senile plaque.Senile dementia belongs to the categories such as Chinese medicine " dementia ", " dementia ", " forgetful ".Senile dementia position is at brain, closely related with the function of kidney, the heart, liver, spleen etc., and it it is critical only that the effect of kidney and liver.The kidney being the origin of congenital constitution, store essential substances and raw marrow the brain being the reservoir of the marrow, and kidney essense is sufficient, and in QI and blood, Rong Ze is clear-headed and clear-sighted, resourceful；Kidney essense is deficient, and brain is lost and filled, and god becomes homeless foster, then be likely to develop into dementia.Liver controlling conveyance and dispersion, store blood, soul house also, the liver failing to maintain the normal flow of QI, feelings will is unsuccessful, depression of liver-QI, then facilitated burn is cloudy, qi stagnation hemagglutination, and brain network blocks, dysfunction of YANG-orifices and cause dementia.Deficiency of spleen-QI, loses in strong fortune, and essence of water and grain can not be up, and gods are lost and supported, and also may result in senile dementia.Therefore, the gas of the invigorating spleen and kidney heart, nourishes liver, can rouse oneself internal organs function, effectively alleviate senile dementia.

Memory is that experience is crossed the memorize of things, maintenance, reproduced or re-recognize by human brain, and it is by the basis of the senior mental activity such as thinking, the imagination, connects the mental activity of people, is people's basic functions of learning, work and living.As found, oneself recent memory power is much worse than in the past, does not often remember thing, forgetful, forget the thing etc. just done, here it is our hypomnesis of often saying, medically, is called " forgetful ".The traditional Chinese medical science is thought, " heart storing spirit, liver storing soul, lung storing inferior spirit, idea stored in the spleen, kidney hide intelligence ", the thinking activities of people and the five internal organs all have very close relationship.Additionally, " the brain being the house of mentality ", brains are filled, then normal hearing and eyesight, energetic；Brains are hollow, may occur in which hypomnesis.The big factor of forgetful one is empty for heart deficiency of the kidney, and painstaking effort deficiency of kidney-essence is difficult to moisten foster, and painstaking effort dry up, kidney water exhausts thus disarmony between the heart and kidney, may result in forgetful in the course of time." General Treatise on the Cause and Symptoms of Diseases " is discussed: " impairment of the heart caused by overstrain person, before we notice it forgetfulness ".Sun Simiao thinks that the old asthenia of people may result in kidney yang deficiency, cloudy insufficiency of essence, and in the kidney of old people, yang-energy day by day loses few with advancing age, and kidney yang can not be up to, in the heart, may result in forgetful generation." Shengji Zonglu " speech " forgetful disease, this is in having a guilty conscience "." Prescriptions for Universal Relief " cloud: " disease that husband is forgetful, this is in having a guilty conscience, and vim and vigour decline few, and spirit dotes, therefore will turmoil and poor memory are also.Gai Xinzhe, the viscus likened to the monarch in the body, here gods go out, careless for not cautious ponder over hindered, or anxious melancholy crosses damage, and be scared mental confusion, and all causing is disease.Therefore say: anxious worry of being worried is then sad, grieved then forgetful." indicate various emotional factors and injure heart temper blood, thus causing forgetful.Attacking the court of a feudal ruler virtuous in " Longevity and Life Preservation " book, special train relevant " forgetful ", it is believed that having a guilty conscience is the basic reason of forgetful generation, can hold concurrently it is dirty deficient simultaneously, the modes such as prescription listed by it includes tranquilizing by nourishing the heart, heart kidney is ruled together.The traditional Chinese medical science is thought forgetful and can be caused by excess syndrome or deficiency syndrome, and deficiency syndrome are damaged closely related with heart spleen kidney three deficiency of five ZANG-organs, and excess syndrome is then coagulated with stasis blocking expectorant, venation is obstructed in close relations.Therefore the traditional Chinese medical science can adopt the treatment of the method such as activating blood circulation to dissipate blood stasis and dredge the collateral, vital energy benefiting and the kidney invigorating mind calming forgetful, reaches to improve the effect of memory.

Depression is one of disease that whole world mortality rate is the highest.Pathogenic factor is complicated, there is no effective radical cure method at present.Depression is also known as depressive disorder, low for main clinical characteristics with notable and lasting mental state, is the main Types of mood disorders.Depression is equivalent to the categories such as the melancholia of Chinese medicine, insane disease, Bulbus Lilii syndrome, globus hystericus, renal mass, mental confusion.It is more that the etiology and pathogenesis of depression is recognized angle by Chinese medicine, and major part doctor thinks that emotional stimulation and system natural endowment are two big Etiologicals of depression.Depression etiology and pathogenesis is complicated, but always, depression and stagnation of QI easypro with feelings will is for its main cause, and pathological changes involves the vital organs of the human body, but closely related with the function of the organ such as liver,spleen,kidney, the heart.Strongly fragrant why rising, first " stagnation of QI ", continue then " blood stagnation ", then occurs that Mental fatigue gas is weak, and " god is strongly fragrant " runs through depression overall process.During depressed card morbidity, feelings will is unsuccessful, and stagnation of liver-QI presses down spleen, consumes sad gas, and nutrient blood gradually consumes, and the heart is become homeless foster, and god becomes homeless Tibetans, and namely what is called melancholy is overtaxed one's nerves；Chronic depression impairing the spleen, diet reduces, biochemical passive then insufficiency of vital energy and blood, deficiency of both the heart and spleen；The easy impairment of YIN blood of strongly fragrant fire-transformation of a specified duration, involves in kidney, the troubled thoughts impairment of mentality, kidney storing will thus be worried and be also impairment of the kidney.Heart governing blood and vessels, again main gods, kidney controlling essence storage, marrow fills in brain, and the motive is also led in brain.The heart, spleen, kidney are both empty, then asthenia of essence and blood, and essence is activating QI not, unit's god's virtual loss, and shape is shown in that how quick slow movement, vexed uneasiness, interest is dull or suspicious etc..As can be seen here, alleviating stagnation of liver-QI, reinforcing spleen and kidney can effectively treat depression.

Anxiety neurosis is a kind of constant tension, worry, the frightened or ictal terrified dysthymic disorder for feature, with behavior characteristicss such as vegetative system symptom and motion uneasinesses, including generalized anxiety disorder and panic disorder.Anxiety neurosis is common clinical, frequently-occurring disease, and along with society and expanding economy, the sickness rate of anxiety neurosis has the trend risen year by year.Anxiety neurosis belongs to Chinese medicine emotional disorder category, contains the categories such as " melancholia ", " in terror ", " being insomnia ", " palpitation with fear ", " palpitation with a distress feeling ", " Renal mass disease "."Nei Jing" has cloud, and ", without being leaned on, god, without being returned, considers nothing and is determined for the frightened then heart, therefore disturbance of QI." " General Treatise on the Cause and Symptoms of Diseases ": " husband sensation of gas rushing person, the pneumatosis of kidney.Arise from terrified, be worried and given birth to.If terrified ... mind wound is dynamic, and QI-mass is in kidney, and upstream under gas, such as running quickly of globefish, therefore says renal mass." " etiology based on plain questions ": " frightened, heart soldier is dynamic and not peaceful.The owner of the house where a fire started is in dynamic, therefore heart-fire heat is very also, so-called probably then susceptible to fright person, is probably then impairment of the kidney and water declines, heart-fire is from very, therefore susceptible to fright is also." organ such as morbidity and liver, the heart, kidney of anxiety neurosis is in close relations.Anxiety neurosis basic pathogenesis is many to be reached by the liver failing to maintain the normal flow of QI bar, and the stagnation of QI does not dissipate, the raw expectorant of fire-transformation, and phlegm-fire is disturbed and heart and gallbladder, and with the passing of time gas, expectorant, fire pent up, and consumption impairment of QI is cloudy, and caused by heart and gallbladder two void, simulataneous insufficiency and excessive, the course of disease is touching not to heal.As can be seen here, alleviating stagnation of liver-QI, reinforcing spleen and kidney, nourishing YIN to lower pathogenic fire can effectively treat anxiety neurosis.

In prior art, the medicine for the treatment of insomnia, senile dementia, raising ability of learning and memory, depression and anxiety neurosis is a lot, but its therapeutic effect is undesirable, and side effect is big.Such as treat the Western medicine of insomnia, it is easy to cause liver injury；The Western medicine for the treatment of senile dementia, can only alleviate the cognitive disorder of early stage patient, it is provided that the symptom improvement result of appropriateness, it is impossible to stoping the progress of the state of an illness, some drugs even produces to promote side effect faggoty；The conventional antidepressants impact on blood pressure and the toxicity to heart are bigger；Antianxiety drugs dosage is too high, the course for the treatment of is long, all can cause drug dependence.And, market is also absent from treating the medicine of above several diseases simultaneously, multi-medicament is taken simultaneously exists more incompatibility.From theory of Chinese medical science, there is common pathogenesis in above disease, so using tcm therapy directly can act on for the cause of disease, and the above several diseases for the treatment of simultaneously.

In the prior art, Chinese patent CN102657819B discloses a kind of Chinese medicine composition for treating depression, and said composition is using 15 taste Chinese medicines as crude drug.Chinese medicine composition component like this is more, and quality is wayward, and technical process is industrialization not easily, and the scope of application is single.

Summary of the invention

It is an object of the invention to from suppressing liver-YANG, the Chinese medicine angle that QI invigorating is allayed excitement provide a kind of new while improve sleep, the preparation method improving senile dementia, antidepressant, Chinese medicine composition antianxity.

This Chinese medicine composition is prepared by the crude drug composition of weight portion:

Rhizoma Gastrodiae 1-10 weight portion, Radix Paeoniae Alba 2-12 weight portion, Radix Et Caulis Acanthopanacis Senticosi 1-8 weight portion, Semen Ziziphi Spinosae 1-10 weight portion.

Preferably, the crude drug of described compositions consists of:

Rhizoma Gastrodiae 1.2-5 weight portion, Radix Paeoniae Alba 6-11 weight portion, Radix Et Caulis Acanthopanacis Senticosi 1.1-4 weight portion, Semen Ziziphi Spinosae 4.1-8 weight portion；

Or

Rhizoma Gastrodiae 5.2-9.5 weight portion, Radix Paeoniae Alba 2.1-5 weight portion, Radix Et Caulis Acanthopanacis Senticosi 4.1-8 weight portion, Semen Ziziphi Spinosae 1.1-6 weight portion；

Or

Rhizoma Gastrodiae 5.2-9.5 weight portion, Radix Paeoniae Alba 2.1-5 weight portion, Radix Et Caulis Acanthopanacis Senticosi 4.1-8 weight portion, Semen Ziziphi Spinosae 4.1-8 weight portion.

It is furthermore preferred that the crude drug of described compositions consists of:

Rhizoma Gastrodiae 1 weight portion, the Radix Paeoniae Alba 2 weight portion, Radix Et Caulis Acanthopanacis Senticosi 3 weight portion, Semen Ziziphi Spinosae 1 weight portion；

Or

Rhizoma Gastrodiae 3 weight portion, the Radix Paeoniae Alba 7 weight portion, Radix Et Caulis Acanthopanacis Senticosi 9 weight portion, Semen Ziziphi Spinosae 3 weight portion；

Or

Rhizoma Gastrodiae 6 weight portion, the Radix Paeoniae Alba 4 weight portion, Radix Et Caulis Acanthopanacis Senticosi 4 weight portion, Semen Ziziphi Spinosae 5 weight portion；

Or

Rhizoma Gastrodiae 7.5 weight portion, the Radix Paeoniae Alba 5 weight portion, Radix Et Caulis Acanthopanacis Senticosi 6 weight portion, Semen Ziziphi Spinosae 5 weight portion.

Preparing crude drug according to above-mentioned weight portion, described preparation method comprises the steps:

Step 1, take Rhizoma Gastrodiae, add 20-90% alcohol reflux, obtain Rhizoma Gastrodiae extract fine powder；

Step 2, take the Radix Paeoniae Alba, add 30-80% alcohol reflux, obtain Radix Paeoniae Alba extract fine powder；

Step 3, take Radix Et Caulis Acanthopanacis Senticosi, boiling, obtain Radix Et Caulis Acanthopanacis Senticosi extract powder；

Step 4, take Semen Ziziphi Spinosae, boiling, obtain Semen Ziziphi Spinosae extract powder；

Step 5, take Rhizoma Gastrodiae extract powder, Radix Paeoniae Alba extract powder, Radix Et Caulis Acanthopanacis Senticosi extract powder, Semen Ziziphi Spinosae extract powder mixing, conventionally technique, makes clinical or pharmaceutically acceptable tablet, capsule, powder, pill, granule, soft extract with bee honey agent, oral liquid, slow releasing preparation, quick releasing formulation etc..

One provided by the invention has suppressing liver-YANG, QI invigorating is allayed excitement, improve sleep, the preparation method improving the Chinese medicine composition of senile dementia, antidepressant effect, and the method is preferably as follows step:

Step 1, take Rhizoma Gastrodiae, be ground into coarse powder, add 50-80% alcohol reflux 1-3 time, alcohol adding amount is 10-20 times, each 1-2 hour, decompression recycling ethanol (pressure 0.05MPa, temperature 60 C), concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) becomes thick paste (when 60 DEG C, relative density is 1.20～1.25), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain the dry cream of extract, pulverize, cross 80 mesh sieves, obtain Rhizoma Gastrodiae extract fine powder；

Step 2, take the Radix Paeoniae Alba, be ground into coarse powder, add 40-60% alcohol reflux 1-3 time, alcohol adding amount is 10-20 times, each 1-2 hour, decompression recycling ethanol (pressure 0.05MPa, temperature 60 C), concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) becomes thick paste (when 60 DEG C, relative density is 1.20～1.30), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain extract dried cream powder, pulverize, cross 80 mesh sieves, obtain Radix Paeoniae Alba extract fine powder；

Step 3, take Radix Et Caulis Acanthopanacis Senticosi, be broken into thick block, soak 2-4h, decocting and extract 1-3 time, amount of water is 5-10 times, each decocting time 1-2 hour, filtering, merging filtrate obtains Radix Et Caulis Acanthopanacis Senticosi water cooking liquid, concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) become thick paste (when 60 DEG C, relative density is 1.20～1.30), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain the dry cream of extract, pulverize, cross 80 mesh sieves, obtain Radix Et Caulis Acanthopanacis Senticosi extract fine powder；

Step 4, take Semen Ziziphi Spinosae, broken, soak 2-4h, decocting and extract 1-3 time, amount of water is 5-10 times, each decocting time 1-2 hour, filter merging filtrate, obtain Semen Ziziphi Spinosae water cooking liquid, concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) become thick paste (when 60 DEG C, relative density is 1.20～1.30), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain the dry cream of extract, pulverize, cross 80 mesh sieves, obtain Semen Ziziphi Spinosae extract fine powder；

Step 5, take Rhizoma Gastrodiae extract fine powder, Radix Paeoniae Alba extract fine powder, Radix Et Caulis Acanthopanacis Senticosi extract fine powder, Semen Ziziphi Spinosae extract fine powder, carboxymethylstach sodium mixing, 60% ethanol moistens soft material processed, 20 mesh sieve granules, 70 DEG C dry, granulate, adds magnesium stearate mix homogeneously, makes tablet.

Chinese medicine composition provided by the invention is made up of Rhizoma Gastrodiae, the Radix Paeoniae Alba, Radix Et Caulis Acanthopanacis Senticosi, Semen Ziziphi Spinosae, Rhizoma Gastrodiae suppressing the hyperactive liver to relieve the wind syndrome, suppressing liver-YANG in formula；The Radix Paeoniae Alba is nourished blood yin fluid astringing, easing the affected liver resolving depression, tonifying liver blood, dispersing liver-energy；Radix Et Caulis Acanthopanacis Senticosi replenishing QI to invigorate the spleen, tonifying the kidney for tranquilization, can enriching spleen-QI, the kidney invigorating gas, lung benefiting gas, reinforcing the heart gas, it is possible to control insomnia, control forgetful, anti-aging；Semen Ziziphi Spinosae nourish heart the liver benefiting, calm the nerves and promote the production of body fluid, the moon that can nourish heart, tonifying liver gas, control insomnia.All medicines share, and easing the affected liver body, dispersing liver-energy, subduing the hyperfunction of the liver sun, the moon that nourishes heart, the kidney invigorating gas, spleen invigorating gas are allayed excitement by suppressing liver-YANG, QI invigorating, easing the affected liver resolving depression, it is possible to control insomnia, anti-aging, control forgetful, solution stagnation of liver-QI, antidepressant, anxiety.

The present invention has an advantage that

1. the present invention selects natural plant to be raw material, adopts the comprehensive function of each taste Chinese medicine to improve sleep, improves senile dementia, improves ability of learning and memory, antidepressant, anxiety, without any side effects, and will not produce dependency.

2. the Chinese medicine composition that the preparation method of the present invention obtains is from suppressing liver-YANG, and the Chinese medicine angle that QI invigorating is allayed excitement is set out, and has improvement sleep simultaneously, improves senile dementia, improves ability of learning and memory, antidepressant, effect antianxity, applied widely.

3. being found by pharmacological evaluation and clinical observation, insomnia, senile dementia, hypomnesis, depression and anxiety neurosis are had good effect and difficulty in relapse after healing, short treating period by the Chinese medicine composition that the preparation method of the present invention obtains.

Detailed description of the invention

Embodiment 1

Rhizoma Gastrodiae 1kg, Radix Paeoniae Alba 2kg, Radix Et Caulis Acanthopanacis Senticosi 3kg, Semen Ziziphi Spinosae 1kg.

Take above-mentioned raw materials medicine, be prepared via a method which:

1, take Rhizoma Gastrodiae, be ground into coarse powder, add 70% alcohol reflux 2 times, alcohol adding amount respectively 10,10 times, each 2 hours, decompression recycling ethanol (pressure 0.05MPa, temperature 60 C), concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) becomes thick paste (when 60 DEG C, relative density is 1.20～1.25), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain the dry cream of extract, pulverize, cross 80 mesh sieves, obtain Rhizoma Gastrodiae extract fine powder；

2, take the Radix Paeoniae Alba, be ground into coarse powder, add 50% alcohol reflux 2 times, alcohol adding amount respectively 10,10 times, each 1.5 hours, decompression recycling ethanol (pressure 0.05MPa, temperature 60 C), concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) becomes thick paste (when 60 DEG C, relative density is 1.20～1.30), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain extract dried cream powder, pulverize, cross 80 mesh sieves, obtain Radix Paeoniae Alba extract fine powder；

3, take Radix Et Caulis Acanthopanacis Senticosi, be broken into thick block, soak 2h, decoct and extract 2 times, amount of water respectively 10,10 times, 2 hours first times, second time 1 hour, filters, and merging filtrate obtains Radix Et Caulis Acanthopanacis Senticosi water cooking liquid, concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) becomes thick paste (when 60 DEG C, relative density is 1.20～1.30), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain the dry cream of extract, pulverize, cross 80 mesh sieves, obtain Radix Et Caulis Acanthopanacis Senticosi extract fine powder；

4, Semen Ziziphi Spinosae is taken, broken, soak 2h, decoct and extract 2 times, amount of water respectively 10,8 times, 2 hours first times, second time 1 hour, filters merging filtrate, obtains Semen Ziziphi Spinosae water cooking liquid, concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) becomes thick paste (when 60 DEG C, relative density is 1.20～1.30), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain the dry cream of extract, pulverize, cross 80 mesh sieves, obtain Semen Ziziphi Spinosae extract fine powder；

5, taking Rhizoma Gastrodiae extract fine powder, Radix Paeoniae Alba extract fine powder, Radix Et Caulis Acanthopanacis Senticosi extract fine powder, Semen Ziziphi Spinosae extract fine powder mix homogeneously, technique makes tablet routinely.

Embodiment 2

Rhizoma Gastrodiae 3kg, Radix Paeoniae Alba 7kg, Radix Et Caulis Acanthopanacis Senticosi 9kg, Semen Ziziphi Spinosae 3kg.

Take above-mentioned raw materials medicine, be prepared via a method which:

1, take Rhizoma Gastrodiae to pulverize, cross 80 mesh sieves, obtain Rhizoma Gastrodiae fine powder；

4, take Semen Ziziphi Spinosae, pulverize, cross 80 mesh sieves, obtain Semen Ziziphi Spinosae fine powder；

5, take Rhizoma Gastrodiae fine powder, Radix Paeoniae Alba extract fine powder, Radix Et Caulis Acanthopanacis Senticosi extract powder, Semen Ziziphi Spinosae fine powder mix homogeneously, make capsule through common process.

Embodiment 3

Rhizoma Gastrodiae 6kg, Radix Paeoniae Alba 4kg, Radix Et Caulis Acanthopanacis Senticosi 4kg, Semen Ziziphi Spinosae 5kg.

Take above-mentioned raw materials medicine, be prepared via a method which:

5, take Rhizoma Gastrodiae extract fine powder, Radix Paeoniae Alba extract fine powder, Radix Et Caulis Acanthopanacis Senticosi extract fine powder, Semen Ziziphi Spinosae extract fine powder mix homogeneously, make granule through common process.

Embodiment 4

Rhizoma Gastrodiae 7.5kg, Radix Paeoniae Alba 3kg, Radix Et Caulis Acanthopanacis Senticosi 6kg, Semen Ziziphi Spinosae 6kg.

Take above-mentioned raw materials medicine, be prepared via a method which:

5, take Rhizoma Gastrodiae extract fine powder, Radix Paeoniae Alba extract fine powder, Radix Et Caulis Acanthopanacis Senticosi extract fine powder, Semen Ziziphi Spinosae extract fine powder mix homogeneously, make powder through common process.

Embodiment 5

Rhizoma Gastrodiae 1.2kg, Radix Paeoniae Alba 2.4kg, Radix Et Caulis Acanthopanacis Senticosi 3.5kg, Semen Ziziphi Spinosae 1.4kg.

Take above-mentioned raw materials medicine, be prepared via a method which:

5, take Rhizoma Gastrodiae fine powder, Radix Paeoniae Alba extract fine powder, Radix Et Caulis Acanthopanacis Senticosi extract powder, Semen Ziziphi Spinosae fine powder mix homogeneously, make tablet through common process.

Embodiment 6

Rhizoma Gastrodiae 3kg, Radix Paeoniae Alba 8kg, Radix Et Caulis Acanthopanacis Senticosi 10kg, Semen Ziziphi Spinosae 4kg.

Take above-mentioned raw materials medicine, be prepared via a method which:

5, take Rhizoma Gastrodiae extract fine powder, Radix Paeoniae Alba extract fine powder, Radix Et Caulis Acanthopanacis Senticosi extract fine powder, Semen Ziziphi Spinosae extract fine powder mix homogeneously, make capsule through common process.

Embodiment 7

Rhizoma Gastrodiae 1kg, Radix Paeoniae Alba 3kg, Radix Et Caulis Acanthopanacis Senticosi 4kg, Semen Ziziphi Spinosae 5kg.

Take above-mentioned raw materials medicine, be prepared via a method which:

Embodiment 8

Rhizoma Gastrodiae 3.5kg, Radix Paeoniae Alba 3kg, Radix Et Caulis Acanthopanacis Senticosi 6kg, Semen Ziziphi Spinosae 6kg.

Take above-mentioned raw materials medicine, be prepared via a method which:

5, take Rhizoma Gastrodiae fine powder, Radix Paeoniae Alba extract fine powder, Radix Et Caulis Acanthopanacis Senticosi extract powder, Semen Ziziphi Spinosae fine powder mix homogeneously, make powder through common process.

Experimental example 1

Consulting lot of documents, and in conjunction with theory of Chinese medical science basis, filtering out one group to improving sleep is having the formula better acted on, and its formula consists of: Rhizoma Gastrodiae, Fructus Schisandrae Chinensis, the Radix Paeoniae Alba, Radix Et Caulis Acanthopanacis Senticosi, Caulis Polygoni Multiflori, Semen Ziziphi Spinosae.For simplifying formula composition, design the orthogonal test of one group of 7 factor 2 level, it is preferable that there is the formula composition improving sleep effect.Quadrature factor designs in Table 1.

Table 1 prescription investigates factor level table

1 material and method

1.1 samples: Fructus Schisandrae Chinensis, Rhizoma Gastrodiae, the Radix Paeoniae Alba, Semen Ziziphi Spinosae, Caulis Polygoni Multiflori, Radix Et Caulis Acanthopanacis Senticosi

1.2 laboratory animals: 18～22g Kunming kind health SPF level male mice, are randomly divided into 9 groups (blank group, 8 groups of orthogonal test groups), often group 12.Carry out extending the pentobarbital sodium experiment length of one's sleep, with the length of one's sleep for index, investigate in formula and have the flavour of a drug of better effect to improving to sleep.

1.3 test packets: blank group；8 orthogonal test group respectively orthogonal systems 1: the Rhizoma Gastrodiae+Semen Ziziphi Spinosae+Radix Paeoniae Alba+Radix Et Caulis Acanthopanacis Senticosi+Caulis Polygoni Multiflori+Fructus Schisandrae Chinensis, orthogonal systems 2, Rhizoma Gastrodiae+Semen Ziziphi Spinosae+Radix Paeoniae Alba, orthogonal systems 3, Rhizoma Gastrodiae+Radix Et Caulis Acanthopanacis Senticosi+Caulis Polygoni Multiflori, orthogonal systems 4, Rhizoma Gastrodiae+Fructus Schisandrae Chinensis, orthogonal systems 5, Semen Ziziphi Spinosae+Radix Et Caulis Acanthopanacis Senticosi+Fructus Schisandrae Chinensis, orthogonal systems 6, Semen Ziziphi Spinosae+Caulis Polygoni Multiflori, orthogonal systems 7, the Radix Paeoniae Alba+Radix Et Caulis Acanthopanacis Senticosi, orthogonal systems 8, the Radix Paeoniae Alba+Caulis Polygoni Multiflori+Fructus Schisandrae Chinensis.

Prepared by 1.4 orthogonal test group medicines:

Prepared by Rhizoma Gastrodiae extract: take Rhizoma Gastrodiae, it is ground into coarse powder, add 70% alcohol reflux 2 times, alcohol adding amount respectively 10,10 times, each 2 hours, decompression recycling ethanol (pressure 0.05MPa, temperature 60 C), concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) become thick paste (when 60 DEG C, relative density is 1.20～1.25), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain the dry cream of extract, calculate Rhizoma Gastrodiae extract paste-forming rate, pulverize, cross 80 mesh sieves, obtain Rhizoma Gastrodiae extract fine powder, stand-by；

Prepared by Caulis Polygoni Multiflori extract: take Caulis Polygoni Multiflori, it is broken into thick block, soak 2h, decoct and extract 2 times, amount of water respectively 10,8 times, 2 hours first times, second time 1 hour, filters, and merging filtrate obtains Radix Et Caulis Acanthopanacis Senticosi water cooking liquid, concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) become thick paste (when 60 DEG C, relative density is 1.20～1.30), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain the dry cream of extract, calculate Caulis Polygoni Multiflori extract paste-forming rate, pulverize, cross 80 mesh sieves, obtain Caulis Polygoni Multiflori extract fine powder, stand-by；

Prepared by Radix Paeoniae Alba extract: take the Radix Paeoniae Alba, it is ground into coarse powder, add 50% alcohol reflux 2 times, alcohol adding amount respectively 10,10 times, each 1.5 hours, decompression recycling ethanol (pressure 0.05MPa, temperature 60 C), concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) become thick paste (when 60 DEG C, relative density is 1.20～1.30), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain extract dried cream powder, calculate Radix Paeoniae Alba extract paste-forming rate, pulverize, cross 80 mesh sieves, obtain Radix Paeoniae Alba extract fine powder, stand-by；

Prepared by Fructus Schisandrae Chinensis extrat: take Fructus Schisandrae Chinensis, broken, add 60% alcohol reflux 2 times, alcohol adding amount respectively 10,8 times, each 2 hours, decompression recycling ethanol (pressure 0.05MPa, temperature 60 C), concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) become thick paste (when 60 DEG C, relative density is 1.20～1.25), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain the dry cream of extract, calculate Fructus Schisandrae Chinensis extrat paste-forming rate, pulverize, cross 80 mesh sieves, obtain Fructus Schisandrae Chinensis extrat fine powder, stand-by；

Prepared by Radix Et Caulis Acanthopanacis Senticosi extract: take Radix Et Caulis Acanthopanacis Senticosi, it is broken into thick block, soak 2h, decoct and extract 2 times, amount of water respectively 10,8 times, 2 hours first times, second time 1 hour, filters, and merging filtrate obtains Radix Et Caulis Acanthopanacis Senticosi water cooking liquid, concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) become thick paste (when 60 DEG C, relative density is 1.20～1.30), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain the dry cream of extract, calculate Radix Et Caulis Acanthopanacis Senticosi paste-forming rate, pulverize, cross 80 mesh sieves, obtain Radix Et Caulis Acanthopanacis Senticosi extract fine powder, stand-by；

Prepared by Semen Ziziphi Spinosae extract: take Semen Ziziphi Spinosae, broken, soak 2h, decoct and extract 2 times, amount of water respectively 10,8 times, 2 hours first times, second time 1 hour, filters merging filtrate, obtains Semen Ziziphi Spinosae water cooking liquid, concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) become thick paste (when 60 DEG C, relative density is 1.20～1.30), drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtain the dry cream of extract, calculate Semen Ziziphi Spinosae paste-forming rate, pulverize, cross 80 mesh sieves, obtain Semen Ziziphi Spinosae extract fine powder, stand-by；

1.5 tested materials preparations: each crude drug amount total amount 12g altogether organizing test medicine, each test medicine flavour of a drug are all by the proportions of crude drug amount 1:1, and medicine distilled water (is sterilized) preparation.

1.6 administrations: gastric infusion once a day, mouse stomach volume is 0.2mL/10g Mus weight, and blank group distilled water (sterilizing) replaces tested material, and every day, gavage volume was identical with each tested material group.

2 experimental techniques

Extend the pentobarbital sodium experiment length of one's sleep: successive administration 30d, after last administration 15min, 40mg/kg.BW dosage lumbar injection pentobarbital sodium is pressed to each treated animal, injection volume is 0.1ml/10g.BW, with mice righting reflex loss for sleep index, observe tested material and can extend pentobarbital sodium length of one's sleep.

3 data process

Data process is carried out with SPSS software.Adopting variance analysis, but demand first carries out homogeneity test of variance by the program of variance analysis, variance is neat, calculates F value, F value F_0.05, conclusion: no significant difference between each group mean；F value >=F_0.05, P≤0.05, add up with the comparative approach between two of mean between multiple experimental grouies and a matched group；The data of abnormal or heterogeneity of variance are carried out suitable variable conversion, after meeting normal state or variance requires together, adds up by the data after conversion；If still not up to just too or the neat purpose of variance, using rank test instead and add up after variable conversion.

4 results

By experiment statistics found that, compared with blank group, each administration group all can be obviously prolonged the mice pentobarbital sodium length of one's sleep (P < 0.001, P < 0.01 or P < 0.05), and result is in Table 2.

The impact on pentobarbital sodium Sleeping Time Prolong Ed of the table 2 each test medicine group

: compare with blank group, P < 0.05；*: compare with blank group, P < 0.01

The quadrature analysis length of one's sleep of 5 each single medicinal materials

Table 3 prescription screening L₈(2⁷) orthogonal test table

Table 4 variance analysis (length of one's sleep)

By the length of one's sleep, each single medicinal material being carried out variance analysis, the length of one's sleep is had significant difference, Caulis Polygoni Multiflori E and Fructus Schisandrae Chinensis F there was no significant difference by Rhizoma Gastrodiae A, Semen Ziziphi Spinosae B, Radix Paeoniae Alba C and Radix Et Caulis Acanthopanacis Senticosi D in formula；Therefore simplifying formula is Rhizoma Gastrodiae, Semen Ziziphi Spinosae, the Radix Paeoniae Alba and Radix Et Caulis Acanthopanacis Senticosi.

Experimental example 2 Chinese medicine composition of the present invention improves sleep function test

1 material and method

1.1 samples: the composition tablet prepared in above-described embodiment 1,0.7g/ sheet.

1.2 laboratory animals: 18～22g Kunming kind health SPF level male mice, are randomly divided into 4 groups of blank groups and 3 dosage groups, often group 12.Carry out direct sleep experiments, pentobarbital sodium sub-threshold dose hypnosis experiment, the experiment of barbital sodium Sleep latency respectively and extend the pentobarbital sodium experiment length of one's sleep.

1.3 dosage: recommended dose for adult (by 60kg weighing machine) 4.2g every day, be equivalent to 0.07g/ day/kg body weight.Experiment sets 5 times of human body recommended amounts, 10 times, 30 times, and namely 0.35g/kg.BW every day, 0.70g/kg.BW and 2.10g/kg.BW are basic, normal, high dosage group.Tested material water (is sterilized) preparation, and per os gives once a day, and continuous gavage surveys indices after 30 days.Mouse stomach volume is 0.1mL/10g Mus weight.Setting a solvent control group (0g/kg.BW) simultaneously, replace tested material with distilled water (sterilizing), every day, gavage volume was identical with each tested material group.

1.4 experimental techniques:

1.4.1 direct sleep experiments

Successive administration 30d, after last administration, is judged to entrance sleep with the righting reflex loss of animal more than 1min, and righting reflex recovers to be animal awakening.

1.4.2 pentobarbital sodium sub-threshold dose hypnosis experiment

Successive administration 30d, after last administration 15min, each treated animal presses 35mg/10g.BW dosage injection pentobarbital sodium, and injection volume is 0.1ml/10g.BW, reach more than 1min as sleep criterion using mice righting reflex loss, record each group of sleep number of animals and sleep incidence rate.

1.4.3 barbital sodium Sleep latency experiment

Can successive administration 30d, after last is administered 15min, presses 290mg/kg.BW dosage lumbar injection barbital sodium to each treated animal, and injection dosage is 0.1ml/10g.BW, with mice righting reflex loss for index, observe tested material and shorten barbital sodium Sleep latency.

1.4.4 the pentobarbital sodium experiment length of one's sleep is extended

Successive administration 30d, after last administration 15min, press 40mg/kg.BW dosage lumbar injection pentobarbital sodium to each treated animal, and injection volume is 0.1ml/10g.BW, and with mice righting reflex loss for sleep index, can observation tested material extend pentobarbital sodium length of one's sleep.

1.5 data process

2 results

2.1 impacts on direct sleep

Observing by experiment, each group mice, giving in test medicine 60min, all finds no phenomenon of directly sleeping.Namely composition tablet group and matched group are all without direct syngignoscism, it is possible to carry out the experimentation of 1.2 1.4.

2.2 impacts on pentobarbital sodium sub-threshold dose hypnosis experiment

By result it can be seen that the sleep incidence rate of the middle and high dosage group of composition tablet is all remarkably higher than blank group, and having notable difference between each dosage group, with the increase of tested material dosage, sleep incidence rate is gradually increased.Result is in Table 5.

The table 5 impact on pentobarbital sodium sub-threshold dose mice sleep incidence rate

: compare with 0g/kg.BW group, P < 0.05；*: compare with 0g/kg.BW group, P < 0.01

2.3 impacts on barbital sodium Sleep latency

By result it can be seen that compared with blank group, mice sleep is all had shortening incubation period by each dosage group of composition tablet, in wherein, the Sleep latency of dosage group shortens substantially, significant difference (P < 0.05)；It is notable that high dose group Sleep latency shortens effect, and difference is (P < 0.01) clearly.Result is in Table 6.

The table 6 impact on barbital sodium Sleep latency

2.4 impacts on the pentobarbital sodium induced hypnotic time

Experimental result finds, compared with blank group, high, medium and low dosage group to mouse sleep time all with significantly improve, significant difference (P < 0.01, P < 0.05, P < 0.05).And along with the increase of dosage, the length of one's sleep is more long.Showing that there is facilitation the length of one's sleep extending injection pentobarbital sodium mice by it, result is in Table 7.

The table 7 impact on pentobarbital sodium Sleeping Time Prolong Ed

3 conclusions

Per os gave the composition tablet of mice various dose after 30 days, and 0.70g/kg.BW group can increase pentobarbital sodium sub-threshold dose mice sleep incidence rate (P < 0.05), shortens mice barbital sodium Sleep latency (P < 0.05), extend the mice pentobarbital sodium length of one's sleep (P < 0.05)；Pentobarbital sodium sub-threshold dose mice sleep incidence rate (P < 0.01) can be increased in 2.10g/kg.BW group, shorten mice barbital sodium Sleep latency (P < 0.01), extend the mice pentobarbital sodium length of one's sleep (P < 0.01).

By this experimental result, illustrate that this Chinese medicine composition has and improve sleep function.

Experimental example 3 Chinese medicine composition of the present invention improves senile dementia experiment

1 material and method

1.1 samples: the composition granule prepared in above-described embodiment 3, daily intaking amount is 4.2g/60kg BW.Each dose concentration is prepared with distilled water.

1.2 laboratory animals: ICR kind mice, body weight 18～22g.

1.3 dosage choice: press human body daily intaking amount 4.2g/60kg BW, this EXPERIMENTAL DESIGN blank group model group and basic, normal, high three dosage groups (0.35g/kg BW, 0.70g/kg BW, 1.40g/kg BW).Namely 5 times of human body recommended amounts, 10 times, 20 times are equivalent to.Tested material distilled water is prepared.Once a day, per os gives, and continuous gavage measures indices after 30 days.Mouse stomach volume is 0.1mL/10g Mus weight.Blank group and model group (0g/kg BW), replace tested material with water (sterilizing), and every day, gavage volume was identical with each tested material group.

1.4 test methods

1.4.1D-galactose causes the impact of Aged Mice learning and memory function

Except blank group is injected except normal saline, all the other four groups every day neck dorsal sc injection 1%D-galactose 125mg/kg.BW.If in modeling process, because skin of back repeated localised puncture causes that medicine liquid leakage is serious, injection site can be changed as one sees fit.After modeling 2 weeks, each group gavage gives relative medicine, and modeling simultaneously continues, and treatment continues 30 days.

After treating 25 days, carrying out Morris water maze constant-bearing navigation experiment, totally 5 days, first 1-2 days be the training time, and latter 3 days is learning and memory achievement.Every day at the training point upper and lower noon two periods carries out, training 2 times for every section, be first placed in by mice on platform and adapt to 10s before training, training randomly chooses a place of entry when starting, mice is put in water towards pool wall, observes and record mice and find and climb up platform required time (incubation period).4 training enter water from different place of entry respectively, if mice does not find platform in 120s, are caused platform stable 10s, and incubation period is recorded as 120s, and training interval 60s, records every preclinical meansigma methods of mice every time.Last day removes platform, and in record 2min, mice passes through the number of times in platform site, is designated as traversing times.

1.4.2 D-galactose is caused the impact of SOD, MDA in dementia mice brain, AChE content

After experiment 1.4.1 water maze laboratory terminates, de-cervical vertebra puts to death animal immediately, quickly removes in the ice normal saline after part cerebellar tissue is placed in sterilizing and rinse on ice platform, remove bloodstain, filter paper is dried, and after being precisely weighed, is immersed rapidly by cerebellar tissue in 9 times of normal saline, it is fully ground in ice is abundant by homogenizer, make 10% brain tissue homogenate, with the centrifugal 10min of 3000rpm, take supernatant frozen, SOD, MDA and AChE to be measured.Index determining scheme reference reagent box description.

1.5 data process

2 results

D-galactose is caused the impact of dementia mice ability of learning and memory by 2.1

By result it can be seen that compared with blank group, the average latency time of model group is longer, significant difference (P < 0.01), and the progress of every day is less, learning and memory ability reduces substantially；Compared with model group, the average latency of each dosage group all has shortening, and in wherein, dosage group average latency shortens substantially, significant difference (P < 0.05), and high dose group average latency shortens more apparent, difference highly significant (P < 0.01)；Compared with model group, the latency of each dosage group every day is all in obvious shortening.Illustrate that the learning functionality of dementia mice is had a better role by this Chinese medicine composition.

By the number of times of mice spanning platform position it can be seen that the traversing times of model group is considerably less than blank group；After giving this traditional Chinese medicine composition for treating, the spanning platform number of times of middle dosage group and high dose group increases substantially, significant difference (P < 0.05).Illustrate that this Chinese medicine composition can improve the memory function of dementia mice.

Dementia mice learning and memory function is affected by table 8

##: P < 0.01 compared with blank group；

* P < 0.05 compared with model group；* is P < 0.01 compared with model group；

2.2 pairs of D-galactose cause the impact of SOD, MDA in dementia mice brain, AChE content

By result it can be seen that compared with blank group, in model group mouse brain, SOD content reduces substantially, significant difference (P < 0.01)；MDA content raises substantially, significant difference (P < 0.01)；AChE activity reduces substantially, significant difference (P < 0.01)；Compared with model group, the SOD content of each dosage group and AChE activity all have rise in various degree, and MDA content has reduction trend；Wherein, middle dosage group SOD content and AChE activity raise substantially, significant difference (P < 0.05), and MDA content reduces substantially, significant difference (P < 0.05)；In high dose group brain, SOD content and AChE activity raise substantially, difference highly significant (P < 0.01), and MDA content reduces substantially, and difference highly significant (P < 0.01), result is in Table 9.

Table 9 is on the impact of SOD, MDA and AChE content in dementia mice brain

## is P < 0.01 compared with blank group

* P < 0.05, * * wants than P < 0.01 with model group compared with model group

3. conclusion

This result shows, this Chinese medicine composition 0.70g/kg.BW group can be obviously improved dementia mice learning and memory function, increases SOD content in dementia mice brain, reduces MDA content in brain, strengthens AChE activity (P < 0.05)；1.4g/kg.BW group can be obviously improved dementia mice learning and memory function, increases SOD content in dementia mice brain, reduces MDA content in brain, strengthens AChE activity (P < 0.01).

Can pass through to regulate the activity of SOD in brain by this Chinese medicine composition of this description of test, reduce the content of MDA, alleviate the brain injury of mice, strengthen the activity of AChE (acetylcholine esterase) simultaneously, increase the content of AChE in brain, thus improving the memory function of dementia mice, having and improving senile dementia function.

Experimental example 4 Chinese medicine composition antidepressant effect test of the present invention

1 material and method

1.1 samples: sample is the composition granule prepared in above-described embodiment 7.Human body recommends dose to be 4.2g every day, is equivalent to 0.07g/kg BW.

1.2 laboratory animals: 250-270g male Wistar rat.

1.3 dosage choice: laboratory animal is randomly divided into blank group, model group, high, medium and low dosage 5 groups, often group 10.High, medium and low dosage respectively 1.40,0.70 and 0.35g/kg BW, is respectively equivalent to the 20 of human oral dosage form, 10,5 times, and matched group is simultaneously with distilled water gavage, and gavage amount is 1ml/100g.

1.4 test methods:

1.4.1 packet and administration normal rat 50, after 24h prohibits water non-fasting, give 1% sucrose water, measure the consumption in 1h.Be randomly divided into 5 groups according to sucrose water consumption, often group 10, namely Normal group, model group, the basic, normal, high dosage group (0.35g/kg, 0.70g/kg, 1.40g/kg) of this Chinese medicine composition tablet.Modeling is administered simultaneously, and once a day, successive administration 21 days, each group is all administered by 1.0ml/100g body weight, claims weekly a body weight.

1.4.2 5/cage of modeling Normal group is raised, and normal diet is drunk water, and does not give any stimulation.Other 4 groups, 1/cage is raised, and accepts the unpredictable stress stimulation of 21 days, including: frozen water is swum, baking the affected part after applying some drugs, presss from both sides tail, moist raising, overturns round the clock, fasting, prohibit water etc..Often group gives a kind of stimulation every day at random.Every kind stress concrete operation method:

(1) frozen water swimming: put into by animal in the bucket filling 4 DEG C of cold water (frozen water mixing), depth of water 15cm, the toe of rat just can touch drum head, is taken out by animal after 5min.(2) baking the affected part after applying some drugs: oven temperature be adjusted to 45 DEG C constant, animal is put in baking oven, after 5min by animal take out.(3) folder tail: put in fixing cage by rat, expose tail, clamps from 1cm place of root of the tail portion (firmly not excessive, to make rat send and whine) with mosquito forceps, continues 1min.(4) moist raise: morning 8 point, toward mouse cage in, add 200ml water, change to morning 8 next day.(5) overturn round the clock: morning 8 point, rat is put in dark case, turns on lamp illumination at 8 in evening, to early 8 points next day.(6) fasting: 24h fracture.(7) water is prohibited: 24h cuts off the water supply.

1.4.3 body weight and animal behavioral study

Rat body weight each group of rat of test claims weekly a body weight, record, analyzes each group of rat body weight situation of change.

After sucrose water consumption prohibits water every time, measure 1% sucrose water consumption in every treated animal 1h.

Open field test (open-field) opens case height 40cm, length and width and is 80cm, uncovered, perisporium and bottom and is black, and bottom surface is divided into 25 pieces of grids of area equation.Using animal travels number of squares as horizontal anomalous movement score, with upright number of times for Vertical movements score (two liftoff more than the 1cm of forelimb).Every animal carries out 1 time and measures, each 3min.

Animal is put into rat jumping response case endoadaptation environment 3min by Jumping test, immediately after logical 36V alternating current, and animal is shocked by electricity, and normal reaction is to jump onto platform to hide noxious stimulation.Most animals may skip on copper grid again or repeatedly, snaps back again platform, so train 5min after being shocked by electricity, and the number of times that rat is shocked by electricity is errors number.Reform after 24h test, rat is placed on platform, is energized and clocks, the errors number in record 5min.

1.4.4 the impact on CRH, ACTH, CORT content

Radioimmunology (RIA) measures hypothalamus corticotrophin releasing hormone,CRH (CRH), plasma adrenocorticotropic hormone (ACTH), after blood plasma cortisol (CORT) content Behaviors survey terminates, after all animals broken end takes blood, thalamus is taken off rapidly on ice, weigh in rearmounted tissue cryovial with, after liquid nitrogen quick freezing, putting into-70 DEG C of Refrigerator stores to measuring.Use corticotrophin releasing hormone,CRH radioimmunological kit, thyroliberin radioimmunological kit, and plasma corticosterone radioimmunological kit, test hypothalamus CRH, plasma ACTH, plasma CO RT content.

1.4.5 the impact on monoamine neurotransmitter

Stimulate through the chronic stresses of 21 days, after rat after modeling is carried out behavioral indexes mensuration, animal sacrificed by decapitation, peel off rapidly brain on ice, take Hippocampus, with, after liquid nitrogen quick freezing, putting into-70 DEG C of Refrigerator stores to measuring in rearmounted tissue cryovial of weighing.Weight according to cerebral tissue, adds lysate (0.01% cysteine, 0.2mol/LHClO₄, 0.5mmol/LNa₂EDTA), mark in adding in lysate, every 10mg tissue adds 100 μ l lysates, and ultrasonication, homogenate 15000rpm, 30min, 4 DEG C, supernatant same number of revolutions again is centrifuged 30min, 0.22 μm of frit, takes 20 μ l and detects.

2 results

2.1 impacts on depression rat body weight

From result, in modeling process, rat body weight increasess slowly, compared with model group rats, blank group rat increases substantially in modeling the 8th day, the modeling body weight of the 15th day, significant difference (P < 0.05), the 21st day body weight of modeling increases to be exempted from clearly, difference highly significant (P < 0.01).The body weight relatively model group of each group slightly raises trend, and difference not notable (P > 0.05), in Table 10.

Table 10 on the impact of depression rat body weight (N=10,)

Note: * compared with model group, P < 0.05, * * compared with model group, P < 0.01

2.2 impacts on depression rat sucrose water consumption

By result it can be seen that model group rats sucrose water consumption substantially reduces.Compared with model group, dosage group in blank group, this Chinese medicine composition, high dose group sucrose water consumption increase substantially, significant difference (P < 0.05), result is in Table 11.

Table 11 on depression demonstrate,prove rat sucrose water consumption impact (N=10,)

2.3 on the ethological impact of depression rat

By result it can be seen that after modeling, the desire of finding out of unknown things is reduced by model group rats, spacious case test level motion and the number of times moved both vertically substantially reduce.Compared with model group rats, high dose group rat horizontal movement and the number of times that moves both vertically go up substantially, significant difference (P < 0.05).

After modeling, model group's learning and memory ability reduces substantially, and the errors number of Jumping test and response time raise substantially.After drug treatment, compared with model group, the middle dosage group reaction time reduces substantially, significant difference (P < 0.05), high dose group errors number reduces substantially (P < 0.05), response time reduces clearly, and significant difference (P < 0.01), result is in Table 12.

Table 12 on the ethological impact of depression rat (N=10,)

2.3 on depression rat hypothalamus CRH, plasma ACTH, CORT content impact

By result it can be seen that after modeling, in rat hypothalamus, CRH content has and increases.Compared with model group, in high dose group, middle dosage group hypothalamus, the content of CRH reduces substantially, significant difference (P < 0.05).

After modeling, in model group rats blood plasma, ACTH and CORT content raises substantially, compared with model group, high dose group plasma ACTH and CORT reduce substantially, significant difference (P < 0.05), middle dosage group and low dose group plasma ACTH and CORT content slightly reduce trend, but difference is inconspicuous, and result is in Table 13.

Table 13 on depression rat hypothalamus CRH, plasma ACTH, CORT content impact (N=10,)

Note: * compared with model group, P < 0.05

2.4 impacts on Monoamines DA and 5-HT content

By result it can be seen that after modeling, monoamine neurotransmitter DA content dramatic drop-off in model group Hippocampus.Compared with model group, 0.7g/kgDA content raises substantially, significant difference (P < 0.05)；1.4g/kgDA content raises clearly, significant difference (P < 0.01).

After modeling, after modeling, 5-HT content dramatic drop-off in model group Hippocampus.Compared with model group, 1.4g/kg5-HT content raises substantially, and significant difference (P < 0.05), result is in Table 14.

Table 14 on the impact of depression rat DA and 5-HT content (N=10,)

Note: * compared with model group, P < 0.05

3 results

This Chinese medicine composition can improve behavioristics's result of depression model rat, improves the memory function of rat；Reduce CRH content in hypothalamus, reduce CORT and ACTH content in blood plasma, increase the content of monoamine neurotransmitter DA and 5-HT in Hippocampus, there is antidepressant effect.

This experimental result illustrates that this Chinese medicine composition has antidepressant effect.

Experimental example 5 Chinese medicine composition angst resistance effect test of the present invention

1 material and method

1.1 samples: the compositions powder prepared in above-described embodiment 8, daily intaking amount is 4.2g/60kg BW, amounts to 0.07g/kg BW.

1.2 laboratory animals: male ICR mouse 48.It is randomly divided into 4 groups.

1.3 dosage choice: press human body daily intaking amount 4.2g/60kg BW, this EXPERIMENTAL DESIGN matched group and basic, normal, high three dosage groups, respectively 0.35g/kg BW every day, 0.70g/kg BW, 1.40g/kg BW.Namely 5 times of human body recommended amounts, 10 times, 20 times are equivalent to.Tested material pure water is prepared.Once a day, per os gives, and continuous gavage measures indices after 14 days.Mouse stomach volume is 0.1mL/10g Mus weight.Setting a blank group (0g/kg BW) simultaneously, replace tested material with pure water, every day, gavage volume was identical with each tested material group.

1.4 test methods

1.4.1 the impact of mouse elevated plus-maze behavior

After successive administration is treated 10 days, after administration, 1h starts Behavior Test, and all tests all carry out at 8:00 14:00.Before test, mice is placed individually in animal box, it is allowed to freely movable 5min, during test, mice is placed in the central area of Elevated plus-maze, making its head just to open arms, in record 5min, arm number of times and the time of staying are closed in mice entrance open arms number of times and the time of staying, entrance respectively.Using enter opening and closing now with enter the percentage ratio (OE) of each arm total degree, activity time in open arms and enter the percentage ratio (OT) of total activity time of each arm as the inspection target of angst resistance effect.In test process, mice one foot exits a certain arm and then represents this movable completing, cleaning labyrinth after every time having tested, then tests next time.

1.4.2 the impact that mice light and shade case rows is

Successive administration is treated 10 days, and after last is administered, 1h starts testing experiment.Mice is placed in during test the camera-lucida central authorities of light and shade case, and head, towards camera bellows, is recorded mice in 5min and is worn case number of times and the time in camera-lucida activity after release.

1.4.3 the impact on mice autonomic activities

After successive administration is treated 10 days, 1h after last administration, carry out testing experiment.During test, mice is put into adaptation 10min in autonomic activities instrument, the autonomic activities number of times in record mice 5min.

1.4.4 nikethamide is caused the impact of mice convulsion

Successive administration is treated 10 days, and after last administration 1h, intraperitoneal injection nikethamide 350mg/kg, mice is placed in cage, records dead mouse number in convulsions incubation period of each group of mice and 2h respectively.

1.5 data process

Data process is carried out with SPSS software.Adopting variance analysis, but need to first carry out homogeneity test of variance by the program of variance analysis, variance is neat, calculates F value, F value < F_0.05, conclusion: no significant difference between each group mean；F value >=F_0.05, P≤0.05, add up with the comparative approach between two of mean between multiple experimental grouies and a matched group；The data of abnormal or heterogeneity of variance are carried out suitable variable conversion, after band meets normal state or variance requires together, adds up by the data after conversion；If purpose still neat not up to normal state or variance after variable conversion, use rank test instead and add up.

2 result of the tests

2.1 impacts on mouse elevated plus-maze behavior

By result it can be seen that compared with blank group, 0.7g/kg, 1.4g/kg can substantially increase mouse elevated plus-maze OT and OE value, significant difference (P < 0.05), and in dose-effect relationship, point out it to have angst resistance effect, and result is in Table 15.

The table 15 impact on mouse elevated plus-maze behavior

* P < 0.05 compared with blank group

2.2 impacts being on mice light and shade case rows

By result it can be seen that compared with blank group, 0.7g/kg, 1.4g/kg can substantially increase mice and enter the number of times of camera-lucida and in activity time of camera-lucida, significant difference (P < 0.05), and in dose-effect relationship, prompting has angst resistance effect, and result is in Table 16.

The impact that mice light and shade case rows is by table 16

*: with 0 blank group than P < 0.05

2.3 impacts on mice autonomic activities

By result it can be seen that compared with blank group, 1.4g/kg can substantially reduce the autonomic activities number of times of mice, significant difference (P < 0.05), and is obvious dose-effect relationship.Showing have angst resistance effect, result is in Table 17.

The table 17 impact on mice autonomic activities

* P < 0.05 compared with blank group

2.4 impacts that nikethamide caused mice convulsion

From result, compared with blank group, 1.4g/kg can substantially reduce nikethamide and cause the incubation period of mice convulsion, significant difference (P < 0.05), can reduce because injection nikethamide causes the mortality rate of mice simultaneously, and in obvious dose-effect relationship.Showing have angst resistance effect, result is in Table 18.

Nikethamide is caused the impact of mice convulsion by table 18

* P < 0.05 compared with blank group

3. conclusion

By above-mentioned result of the test it can be seen that 0.7g/kg, 1.4g/kg can dramatically increase mouse elevated plus-maze OT and OE value, increase the number of times of mice entrance camera-lucida and in the activity time of camera-lucida, significant difference (P < 0.05)；1.4g/kg can substantially reduce mice autonomic activities number of times, extends because injection nikethamide causes the incubation period of mice convulsion and reduces mortality rate, significant difference (P < 0.05).

By this Chinese medicine composition of this description of test, there is effect antianxity.

Obviously, above-described embodiment is only for clearly demonstrating example, and is not the restriction to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here without also cannot all of embodiment be given exhaustive.And the apparent change thus extended out or variation are still among the protection domain of the invention.

Claims

1. the preparation method treated insomnia, improve senile dementia, improve the Chinese medicine composition of ability of learning and memory, antidepressant, anxiety, nootropic effect of calming the nerves, the crude drug of described compositions consists of:

It is characterized in that, described preparation method comprises the steps:

(1) take Rhizoma Gastrodiae, add 20-90% alcohol reflux, obtain Rhizoma Gastrodiae extract fine powder；

(2) take the Radix Paeoniae Alba, add 30-80% alcohol reflux, obtain Radix Paeoniae Alba extract fine powder；

(3) Radix Et Caulis Acanthopanacis Senticosi is taken, boiling, obtain Radix Et Caulis Acanthopanacis Senticosi extract powder；

(4) Semen Ziziphi Spinosae is taken, boiling, obtain Semen Ziziphi Spinosae extract powder；

(5) Rhizoma Gastrodiae extract powder, Radix Paeoniae Alba extract powder, Radix Et Caulis Acanthopanacis Senticosi extract powder, the mixing of Semen Ziziphi Spinosae extract powder are taken, conventionally technique, makes clinical or pharmaceutically acceptable tablet, capsule, powder, pill, granule, soft extract with bee honey agent, oral liquid, slow releasing preparation, quick releasing formulation etc..

2. preparation method according to claim 1, it is characterised in that described step (1) and (2) particularly as follows:

Respectively with alcohol reflux Rhizoma Gastrodiae, Radix Paeoniae Alba 1-3 time, concentrate after decompression recycling ethanol and respectively obtain Rhizoma Gastrodiae, Radix Paeoniae Alba thick paste, respectively obtain Rhizoma Gastrodiae, the dry cream of Radix Paeoniae Alba extract then through drying under reduced pressure, pulverize respectively, sieve to obtain Rhizoma Gastrodiae, Radix Paeoniae Alba extract fine powder.

3. preparation method according to claim 1, it is characterised in that described step (3) and (4) particularly as follows:

Take Radix Et Caulis Acanthopanacis Senticosi respectively, Semen Ziziphi Spinosae crushes, decoct after soaking and extract 1-3 time, after filtration, merging filtrate obtains Radix Et Caulis Acanthopanacis Senticosi, Semen Ziziphi Spinosae water cooking liquid respectively, Radix Et Caulis Acanthopanacis Senticosi, Semen Ziziphi Spinosae water cooking liquid are carried out concentrating under reduced pressure and become Radix Et Caulis Acanthopanacis Senticosi, Semen Ziziphi Spinosae thick paste, then through respectively obtaining Radix Et Caulis Acanthopanacis Senticosi, the dry cream of Semen Ziziphi Spinosae extract after drying under reduced pressure, pulverize respectively, sieve after obtain Radix Et Caulis Acanthopanacis Senticosi, Semen Ziziphi Spinosae extract fine powder.

4. the preparation method according to Claims 2 or 3, it is characterised in that concentration of alcohol 40-70% when alcohol reflux Rhizoma Gastrodiae, the Radix Paeoniae Alba, alcohol adding amount is 10-20 times, each 1-2 hour；2-4h is soaked in boiling when extracting Radix Et Caulis Acanthopanacis Senticosi, Semen Ziziphi Spinosae, decoct and extract 1-3 time, and amount of water is 5-10 times, each decocting time 1-2 hour；Being condensed into relative density when 60 DEG C is the thick paste of 1.20～1.30.

5. the preparation method according to Claims 2 or 3, it is characterised in that when alcohol reflux Rhizoma Gastrodiae, the Radix Paeoniae Alba or boiling extract Radix Et Caulis Acanthopanacis Senticosi, Semen Ziziphi Spinosae time decompression recycling ethanol pressure 0.05MPa, temperature 60 C；Pressure 0.08MPa when concentrating under reduced pressure becomes thick paste, temperature 60 C；Drying under reduced pressure obtains pressure 0.08Mpa during extract dry cream, temperature 70 C.

6. the preparation method according to any one of claim 1-5, it is characterized in that, described step (5) takes Rhizoma Gastrodiae extract fine powder, Radix Paeoniae Alba extract fine powder, Radix Et Caulis Acanthopanacis Senticosi extract fine powder, Semen Ziziphi Spinosae extract fine powder, carboxymethylstach sodium mixing, 60% ethanol moistens soft material processed, 20 mesh sieve granules, 70 DEG C dry, granulate, add magnesium stearate mix homogeneously, make tablet.

7. the preparation method according to any one of claim 1-6, it is characterised in that described preparation method comprises the steps:

(1) take Rhizoma Gastrodiae, be ground into coarse powder, add 50-80% alcohol reflux 1-3 time, alcohol adding amount is 10-20 times, each 1-2 hour, decompression recycling ethanol pressure 0.05MPa, temperature 60 C, concentrating under reduced pressure pressure 0.08MPa, when temperature 60 C becomes thick paste 60 DEG C, relative density is 1.20～1.25, drying under reduced pressure pressure 0.08Mpa, temperature 70 C, obtains the dry cream of extract, pulverizes, cross 80 mesh sieves, obtain Rhizoma Gastrodiae extract fine powder；

(2) take the Radix Paeoniae Alba, be ground into coarse powder, add 40-60% alcohol reflux 1-3 time, alcohol adding amount is 10-20 times, each 1-2 hour, decompression recycling ethanol pressure 0.05MPa, temperature 60 C, concentrating under reduced pressure pressure 0.08MPa, temperature 60 C becomes thick paste, and (when 60 DEG C, relative density is 1.20～1.30, drying under reduced pressure pressure 0.08Mpa, temperature 70 C, obtains extract dried cream powder, pulverizes, cross 80 mesh sieves, obtain Radix Paeoniae Alba extract fine powder；

(3) take Radix Et Caulis Acanthopanacis Senticosi, be broken into thick block, soak 2-4h, decocting and extract 1-3 time, amount of water is 5-10 times, each decocting time 1-2 hour, filtering, merging filtrate obtains Radix Et Caulis Acanthopanacis Senticosi water cooking liquid, concentrating under reduced pressure pressure 0.08MPa, when temperature 60 C becomes thick paste 60 DEG C, relative density is 1.20～1.30, drying under reduced pressure pressure 0.08Mpa, temperature 70 C, obtain the dry cream of extract, pulverize, cross 80 mesh sieves, obtain Radix Et Caulis Acanthopanacis Senticosi extract fine powder；

(4) Semen Ziziphi Spinosae is taken, broken, soak 2-4h, decocting and extract 1-3 time, amount of water is 5-10 times, each decocting time 1-2 hour, filter merging filtrate, obtain Semen Ziziphi Spinosae water cooking liquid, concentrating under reduced pressure pressure 0.08MPa, when temperature 60 C becomes thick paste 60 DEG C, relative density is 1.20～1.30, drying under reduced pressure pressure 0.08Mpa, temperature 70 C, obtain the dry cream of extract, pulverize, cross 80 mesh sieves, obtain Semen Ziziphi Spinosae extract fine powder；

(5) taking Rhizoma Gastrodiae extract fine powder, Radix Paeoniae Alba extract fine powder, Radix Et Caulis Acanthopanacis Senticosi extract fine powder, Semen Ziziphi Spinosae extract fine powder, carboxymethylstach sodium mixing, 60% ethanol moistens soft material processed, 20 mesh sieve granules, 70 DEG C dry, granulate, adds magnesium stearate mix homogeneously, makes tablet.

8. the Chinese medicinal composition preparation that preparation method according to any one of claim 1-7 obtains.