CN105734073A - LCYE gene of ornamental sunflower - Google Patents
LCYE gene of ornamental sunflower Download PDFInfo
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- CN105734073A CN105734073A CN201610242829.1A CN201610242829A CN105734073A CN 105734073 A CN105734073 A CN 105734073A CN 201610242829 A CN201610242829 A CN 201610242829A CN 105734073 A CN105734073 A CN 105734073A
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- lcye
- carotenoid
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- sunflower
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y505/00—Intramolecular lyases (5.5)
- C12Y505/01—Intramolecular lyases (5.5.1)
- C12Y505/01018—Lycopene epsilon-cyclase (5.5.1.18)
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- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
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- Biomedical Technology (AREA)
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Abstract
The invention provides an LCYE gene of an ornamental sunflower.The sequence of the LCYE gene of the ornamental sunflower is shown as SEQ ID NO:1.The LCYE gene has the advantages that the relative activity of lycopene cyclase (LYCB and LYCE) has an important adjusting function on component accumulation of beta-carotenoid and alpha-carotenoid.The invention provides a biosynthetic pathway of carotenoid and a study of key enzymes and genes of the carotenoid.The regulation and control mechanism of synthesis and accumulation of the carotenoid can be illuminated, and regulation and control of synthesis of the plant carotenoid through a genetic engineering means are made possible.
Description
Technical field
Present invention relates particularly to a kind of ornamental sunflower LCYE gene.
Background technology
Carotene is the primary pigments of many plant flowers, fruit etc., sight and the warp to gardening plant
Ji value plays an important role, and the phylloxanthin in its biosynthesis pathway is the antioxygen of a kind of excellent performance
Agent, can pre-anti-aging and senile eyeball retina macular degeneration.Additionally phylloxanthin also is used as cancer
Preventive, life extend the inhibitor of agent, ulcer inhibitor, heart disease and coronary artery disease.Fructus Lycopersici esculenti
The lycopene selectivity of red pigment ε cyclase (lycopene epsiloncyclase, LCYE) catalyzed linear
Cyclisation, forms δ-carotene, is the key enzyme being in branch sites in phylloxanthin metabolic pathway.
LYCB with LYCE gene expression relative strong and weak, determine the β that generated by beta-carotene metabolism-
Kryptoxanthin, cryptoxanthin, neoxathin and the α-kryptoxanthin generated by alpha-carotene metabolism and leaf corpus luteum
Element ratio in carotenoid total amount.
Summary of the invention
The technical problem to be solved in the present invention, is to provide a kind of ornamental sunflower LCYE gene.
The present invention is achieved in that a kind of ornamental sunflower LCYE gene, described ornamental sunflower
LCYE gene order is as shown in SEQ ID NO:1.
It is an advantage of the current invention that: the relative activity pair of lycopene cyclase (LYCB and LYCE)
β-carotenoid (beta-carotene, beta-cryptoxanthin, cryptoxanthin, antheraxanthin and violaxanthin)
Accumulate with α-carotenoid (alpha-carotene, α-kryptoxanthin, pungent xanthin and leaf lutein) component
Play important regulation effect, the invention provides a kind of Carotenoid biosynthetic pathway and key thereof
Enzyme and the research of gene, to illustrate the regulatory mechanism of carotenogenesis and accumulation, and for passing through gene
Engineering means regulation and control Carotenoid in Plants is possibly realized.
Accompanying drawing explanation
The present invention is further illustrated the most in conjunction with the embodiments.
Fig. 1 is that ornamental sunflower LCYE gene cDNA fragment expands electrophoresis pattern.
M:DNA standard molecular weight DL2000；1:HaLCYE intermediate segment amplified production.
Fig. 2 is that ornamental sunflower HaLCYE amino acid identity analyzes schematic diagram.
Fig. 3 is ornamental sunflower HaLCYE and the phyletic evolution of other 12 LCYE albumen of plant divides
Analysis schematic diagram.
Detailed description of the invention
A kind of ornamental sunflower LCYE gene, described ornamental sunflower LCYE gene order such as SEQ ID
Shown in NO:1, it may be assumed that
CGTGCATATGGAAGAGTTAGTCGTGATTTACTTCATGAGGAGTTGCTG
AGAAGGTGTGTGGAGTCAGGCGTTTCATATCTGAGCTCCAAAGTGGA
ACGGATTACTGAAGCTCCAAATGGCCAGAGTCTCATTGAGTGCGAAG
GCAATATCACAATTCCATGCAGGCTTGCTACCGTTGCATCTGGAGCAGC
TTCAGGAAAACTTTTGCAATATGAGCTTGGTGGTCCCCGTGTTTGTGTT
CAAACGGCTTATGGTGTAGAGGTCGAGGTTGAAAGCATACCCTATGAT
CCAAGTCTAATGGTTTTCATGGATTATCGAGACTACACGAAACATAAAT
CTCAATCATTAGAAGCACAATATCCAACATTCCTTTATGTCATGCCCATG
TCTCCAACTAAAGTATTCTTCGAGGAAACCTGTTTGGCTTCAAAAGAA
GCCATGCCTTTCGAGTTATTGAAGACAAAGCTCATGTCAAGATTAAAG
ACAATGGGGATCCGAATAACCAAAACTTATGAAGAGGAATGGTCGTAT
ATTCCAGTAGGCGGATCGTTACCGAACACAGAGCAAAAGAACCTTGC
ATTTGGTGCTGCTGCTAGCATGGTACATCCAGCCAC。
RNA extracts and design of primers
Trizol method is used to extract ornamental sunflower yellow petal total serum IgE.Retrieve according to GenBank
LCYE amino acid consensus sequence design degenerate primer LCYE-F and LCYE-R (being shown in Table 1), RNA
Reverse transcription becomes cDNA the first chain, carries out PCR amplification with cDNA for template, and response procedures is:
94 DEG C of denaturations 3min, 94 DEG C of degeneration 30s, 53 DEG C of annealing 30s, 72 DEG C extend 30s, circulation
35 times, 72 DEG C extend 7min.
Table 1 ornamental sunflower HaLCYE gene clone's the primer
Gel reclaims, and connects carrier and converts DH5 α competent cell, is screened and PCR by blue white macula
After identifying positive colony, order-checking, it is thus achieved that the cDNA fragment of a length of 614bp, encode 204 amino
Acid, as it is shown in figure 1, Fig. 1 is ornamental sunflower LCYE gene cDNA fragment expands electrophoresis pattern,
Wherein, M:DNA standard molecular weight DL2000；1:HaLCYE intermediate segment amplified production.Warp
The LCYE albumen height homology of Blast comparison, the aminoacid sequence of this fragment coding and various plants,
Wherein the highest with Flos Tagetis Erectae (Tagetes erecta) LCYE homology, reach 94%, with Flos Chrysanthemi
(Chrysanthemum boreale) and Caulis et Folium Lactucae sativae (Lactuca sativa) LCYE homology reach 93% respectively,
90%, it is believed that the sequence of acquisition is the conserved sequence of ornamental sunflower LCYE.
Fig. 2 is ornamental sunflower LCYE aminoacid sequence and Flos Tagetis Erectae, Flos Chrysanthemi and the LCYE of Caulis et Folium Lactucae sativae
The Clustal W amino acid identity of albumen analyzes schematic diagram, the as can be seen from Figure 2 ammonia of 4 species
Base acid sequence homology is the highest.Fig. 3 is ornamental sunflower HaLCYE and 12 LCYE of other plant
The phylogenetic analysis schematic diagram of albumen, multiple ratio on the basis of, application MEGA4.0 to 13
The aminoacid sequence of individual LCYE has carried out Phylogenetic analysis.From figure 3, it can be seen that HaLCYE
It is one that the LCYE of Flos Tagetis Erectae, Flos Chrysanthemi and Caulis et Folium Lactucae sativae gathers.
Although the foregoing describing the detailed description of the invention of the present invention, but it is familiar with the technology people of the art
Member should be appreciated that our described specific embodiment is merely exemplary rather than for this
The restriction of bright scope, those of ordinary skill in the art are in the equivalence made according to the spirit of the present invention
Modify and change, all should contain in the scope of the claimed protection of the present invention.
Claims (1)
1. an ornamental sunflower LCYE gene, it is characterised in that: described ornamental sunflower LCYE
Gene order is as shown in SEQ ID NO:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610242829.1A CN105734073A (en) | 2016-04-19 | 2016-04-19 | LCYE gene of ornamental sunflower |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610242829.1A CN105734073A (en) | 2016-04-19 | 2016-04-19 | LCYE gene of ornamental sunflower |
Publications (1)
| Publication Number | Publication Date |
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| CN105734073A true CN105734073A (en) | 2016-07-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610242829.1A Pending CN105734073A (en) | 2016-04-19 | 2016-04-19 | LCYE gene of ornamental sunflower |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104152475A (en) * | 2014-08-18 | 2014-11-19 | 中国烟草总公司郑州烟草研究院 | Tobacco epsilon-lycopene cyclase gene and its application |
| CN105255926A (en) * | 2015-11-25 | 2016-01-20 | 天津大学 | Wolfberry lycopene epsilon-cyclase gene and recombinant vector comprising gene |
-
2016
- 2016-04-19 CN CN201610242829.1A patent/CN105734073A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104152475A (en) * | 2014-08-18 | 2014-11-19 | 中国烟草总公司郑州烟草研究院 | Tobacco epsilon-lycopene cyclase gene and its application |
| CN105255926A (en) * | 2015-11-25 | 2016-01-20 | 天津大学 | Wolfberry lycopene epsilon-cyclase gene and recombinant vector comprising gene |
Non-Patent Citations (6)
| Title |
|---|
| MOEHS CP ET AL: "Analysis of carotenoid biosynthetic gene expression during marigold petal development", 《PLANT MOLECULAR BIOLOGY》 * |
| MOEHS CP ET AL: "Tagetes erecta epsilon cyclase mRNA, complete cds", 《GENBANK登录号：AF251016》 * |
| 吴建设 等: "观赏向日葵八氢番茄红素脱氢酶基因PDS的cDNA克隆与表达特性分析", 《中国细胞生物学学报》 * |
| 邹礼平 等: "植物番茄红素生物合成相关基因的表达调控研究进展", 《安徽农业科学》 * |
| 钟淮钦 等: "观赏向日葵ζ-胡萝卜素脱氢酶基因HaZDS的克隆与表达分析", 《植物遗传资源学报》 * |
| 钟淮钦 等: "观赏向日葵胡萝卜素合成相关酶基因的克隆与表达", 《中国观赏园艺研究进展2013》 * |
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Application publication date: 20160706 |