CN105727363A - 一种尼龙纳米纤维三维交联支架及其制备方法 - Google Patents

一种尼龙纳米纤维三维交联支架及其制备方法 Download PDF

Info

Publication number
CN105727363A
CN105727363A CN201610106719.2A CN201610106719A CN105727363A CN 105727363 A CN105727363 A CN 105727363A CN 201610106719 A CN201610106719 A CN 201610106719A CN 105727363 A CN105727363 A CN 105727363A
Authority
CN
China
Prior art keywords
nylon
nano
fiber
nano fiber
dimensional cross
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610106719.2A
Other languages
English (en)
Inventor
楼涛
汪学军
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao University
Original Assignee
Qingdao University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao University filed Critical Qingdao University
Priority to CN201610106719.2A priority Critical patent/CN105727363A/zh
Publication of CN105727363A publication Critical patent/CN105727363A/zh
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/04—Macromolecular materials
    • A61L31/06—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J3/00—Processes of treating or compounding macromolecular substances
    • C08J3/24—Crosslinking, e.g. vulcanising, of macromolecules
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D—TEXTILES; PAPER
    • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
    • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
    • D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
    • D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
    • D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
    • D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2377/00—Characterised by the use of polyamides obtained by reactions forming a carboxylic amide link in the main chain; Derivatives of such polymers
    • C08J2377/02—Polyamides derived from omega-amino carboxylic acids or from lactams thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2377/00—Characterised by the use of polyamides obtained by reactions forming a carboxylic amide link in the main chain; Derivatives of such polymers
    • C08J2377/06—Polyamides derived from polyamines and polycarboxylic acids

Abstract

本发明涉及一种尼龙纳米纤维三维交联支架及其制备方法，它采用在一定条件下静电纺丝纳米尼龙纤维膜，将纳米尼龙纤维膜在水中高速剪切成短纤维，分样，在?18℃下冷冻成型，冷冻干燥后的样品用聚丙烯酸溶液交联，水洗，冷冻，再经冷冻干燥得到尼龙纳米纤维三维交联支架。尼龙纤维经聚丙烯酸交联后，纤维之间有较好的结合力，支架整体有较好的弹性和整体结构稳定性，可用于组织和器官的修复。

Description

一种尼龙纳米纤维三维交联支架及其制备方法
技术领域
本发明涉及一种尼龙纳米纤维三维交联支架及其制备方法，属于生物医用材料技术领域。
背景技术
尼龙是分子主链上含有重复酰胺基团的热塑性树脂总称，它具有良好的力学性能、耐热性、耐磨损性、耐化学药品性和自润滑性，且摩擦系数低，有一定的阻燃性，易于加工，适于用玻璃纤维和其它填料填充增强改性，在工业中有广泛的应用。尼龙目前在生物材料领域也有广泛的应用，可被制成各种形状的支架用于骨、皮肤和心脏等器官和组织的修复（Suman Das 等， Rapid Prototyping Journal, 2003，9（1）:43-49）。在支架中，尼龙一般都制成实心结构，较少制成多孔网络状的纤维支架，使细胞、血管和神经等难以在支架内部生长。工业中可将尼龙纺丝成各种尺度的纤维，但由于尼龙纤维之间无结合力，将尼龙纤维制成三维多孔网络状的纤维支架是非常困难的。
发明内容
本发明的主要设想是采用静电纺丝的方法制备纳米尼龙薄膜，将尼龙薄膜在水中高速剪切成短纤维，得到尼龙纳米纤维的悬浮液，将悬浮分样后，经冷冻干燥成型，用聚丙烯酸溶液将尼龙纤维交联，水洗后再经冷冻干燥得到尼龙纳米纤维三维交联支架。
本发明中尼龙纳米纤维三维交联支架的制备方法具体如下所述。
(1)静电纺丝纳米尼龙纤维膜，配制一定浓度的尼龙溶液，溶剂为甲酸和水混合物，通以一定的纺丝电压静电纺丝形成纳米尼龙纤维膜。
(2)称取一定量的纳米尼龙纤维膜，加入到定量的水中，在水中高速剪切成短纤维。
(3)分样至称量瓶中，在-18℃下冷冻成型并冻干。
(4)冻干后的样品用10%的聚丙烯酸溶液交联2个小时。
(5)交联后的支架经水洗，-18℃下冷冻成型，冷冻干燥可获得尼龙纳米纤维三维交联支架。
本发明制得的尼龙纳米纤维三维交联支架为白色圆盘，其形状和高度可在分样中采用不同的容器和分样体积调节。尼龙纳米纤维的直径可通过改变静电纺丝的工艺条件调节，尼龙纤维经聚丙烯酸交联后，纤维之间有较好的结合力，支架整体有较好的弹性和整体结构稳定性，可用于组织和器官的修复。该制备工艺简单，相比于单纯的静电纺丝法，该法可容易地制备三维结构的支架。
具体实施方式
下面结合具体实施例，对本发明内容作进一步的说明，但本发明的实现方式并不局限于此。
实施例1：分别取9ml的甲酸和1ml的水，混合均匀，称取1.0g尼龙6，搅拌溶解，静置2小时。将尼龙6溶液加入到注射器中，通以15kV的电压纺丝，收集在接地的铝箔上，可获得尼龙纤维平均直径为80nm的薄膜。称取0.5g纳米尼龙纤维膜，在100ml水中高速剪切成短纤维，分样至称量瓶中，在-18℃下冷冻成型，在冻干机中冻干。冻干后的样品浸泡在10%的聚丙烯酸溶液中交联，时间为2小时，取出交联支架，水洗，-18℃下冷冻，在冻干机中再次冻干可获得尼龙6纳米纤维三维交联支架。
实施例2：分别取8ml的甲酸和2ml的水，混合均匀，称取2.0g尼龙66，搅拌溶解，静置2小时。将尼龙66溶液加入到注射器中，通以20kV的电压纺丝，收集在接地的铝箔上，可获得尼龙纤维平均直径为150nm的薄膜。称取2.0g纳米尼龙66纤维膜，在100ml水中高速剪切成短纤维，分样至称量瓶中，在-18℃下冷冻成型，在冻干机中冻干。冻干后的样品浸泡在10%的聚丙烯酸溶液中交联，时间为2小时，取出交联支架，水洗，-18℃下冷冻，在冻干机中再次冻干可获得尼龙66纳米纤维三维交联支架。

Claims (5)

1.一种尼龙纳米纤维三维交联支架及其制备方法，其特征在于在一定条件下静电纺丝纳米纤维尼龙膜，将纳米尼龙纤维膜在水中高速剪切成短纤维，分样，在-18℃下冷冻成型，冷冻干燥后的样品用聚丙烯酸溶液交联，水洗，冷冻，再经冷冻干燥得到尼龙纳米纤维三维交联支架。
2.根据权利要求1所述的尼龙纳米纤维三维交联支架，其特征在于所使用的尼龙为尼龙6和尼龙66。
3.根据权利要求1所述的尼龙纳米纤维三维交联支架，其特征在于静电纺丝纳米纤维膜时，尼龙浓度为10.0～20.0%之间，溶剂为甲酸和水的混合物，体积比在90:10到80:20之间，纺丝电压在15～20kV之间。
4.根据权利要求1所述的尼龙纳米纤维三维交联支架，其特征在于纳米尼龙纤维在水中高速剪切时的浓度在0.5～2%之间。
5.根据权利要求1所述的尼龙纳米纤维三维交联支架，其特征在于使用的交联剂为10%的聚丙烯酸溶液。
CN201610106719.2A 2016-02-27 2016-02-27 一种尼龙纳米纤维三维交联支架及其制备方法 Pending CN105727363A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610106719.2A CN105727363A (zh) 2016-02-27 2016-02-27 一种尼龙纳米纤维三维交联支架及其制备方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610106719.2A CN105727363A (zh) 2016-02-27 2016-02-27 一种尼龙纳米纤维三维交联支架及其制备方法

Publications (1)

Publication Number Publication Date
CN105727363A true CN105727363A (zh) 2016-07-06

Family

ID=56248639

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610106719.2A Pending CN105727363A (zh) 2016-02-27 2016-02-27 一种尼龙纳米纤维三维交联支架及其制备方法

Country Status (1)

Country Link
CN (1) CN105727363A (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107022794A (zh) * 2017-06-12 2017-08-08 曾林涛 一种自扭曲螺旋、成捻结构微纳米纤维的制备方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030100944A1 (en) * 2001-11-28 2003-05-29 Olga Laksin Vascular graft having a chemicaly bonded electrospun fibrous layer and method for making same
CN1887365A (zh) * 2006-08-01 2007-01-03 四川大学 具有多孔结构的生物材料膜及其制备方法
CN104815355A (zh) * 2015-04-16 2015-08-05 四川大学 表面具有纳米纤维多孔结构的羟基磷灰石/聚酰胺复合生物材料及其制备方法
CN105107022A (zh) * 2015-09-21 2015-12-02 东华大学 一种在湿态下具有压缩弹性的纳米纤维多孔支架的制备方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030100944A1 (en) * 2001-11-28 2003-05-29 Olga Laksin Vascular graft having a chemicaly bonded electrospun fibrous layer and method for making same
CN1887365A (zh) * 2006-08-01 2007-01-03 四川大学 具有多孔结构的生物材料膜及其制备方法
CN104815355A (zh) * 2015-04-16 2015-08-05 四川大学 表面具有纳米纤维多孔结构的羟基磷灰石/聚酰胺复合生物材料及其制备方法
CN105107022A (zh) * 2015-09-21 2015-12-02 东华大学 一种在湿态下具有压缩弹性的纳米纤维多孔支架的制备方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107022794A (zh) * 2017-06-12 2017-08-08 曾林涛 一种自扭曲螺旋、成捻结构微纳米纤维的制备方法
CN107022794B (zh) * 2017-06-12 2019-05-24 苏州克劳丝纳米科技有限公司 一种自扭曲螺旋、成捻结构微纳米纤维的制备方法

Similar Documents

Publication Publication Date Title
US20160136241A1 (en) Methods of preparing and using sericin hydrogel
Han et al. A review: Current status and emerging developments on natural polymer‐based electrospun fibers
Jeong et al. Biodegradable photo-crosslinked alginate nanofibre scaffolds with tuneable physical properties, cell adhesivity and growth factor release
US20080131473A1 (en) Cell-Independent Fabrication of Tissue Equivalents
CN104027846A (zh) 一种非织造材料增强组织工程复合三维支架及其制备方法
CN104018247A (zh) 一种聚氨酯-角蛋白复合纳米纤维管状材料的制备方法
CN111188194B (zh) 一种丝素蛋白/明胶纤维增强壳聚糖复合止血材料的制备方法
CN105641744A (zh) 一种纳微米多尺度壳聚糖聚乳酸复合支架及其制备方法
Yang et al. Minimally invasive bioprinting for in situ liver regeneration
Hosseini Ravandi et al. Application of electrospun natural biopolymer nanofibers
CN103993425A (zh) 一种聚己内酯-角蛋白复合纳米纤维膜的制备方法
Heng et al. Recent research progress of ionic liquid dissolving silks for biomedicine and tissue engineering applications
Wang et al. Elastic fiber-Reinforced silk fibroin scaffold with a Double-Crosslinking network for human ear-shaped cartilage regeneration
US20190282621A1 (en) Blood derivatives composite material, methods of production and uses thereof
CN104497327A (zh) 具有吸水性无交联剂存在的丝素透明质酸复合膜及制备
CN105727363A (zh) 一种尼龙纳米纤维三维交联支架及其制备方法
CN105536075B (zh) 一种纳微米纤维壳聚糖聚乳酸复合支架及其制备方法
US20200215228A1 (en) Collagen microfiber scaffolds
CN104018245B (zh) 一种丝素蛋白/角蛋白复合纳米纤维管状材料的制备方法
CN107412878B (zh) 复合纤维支架及其制备方法
JP6892387B2 (ja) 特に高濃度コラーゲンマトリックスの形成のための、注入可能なコラーゲン懸濁液、その調製方法、及びその使用
CN105749339B (zh) 一种尼龙纳米纤维三维交联大孔支架及其制备方法
WO2019054970A2 (en) PROCESS FOR THE PRODUCTION OF PLGA FIBERS AS TISSUE SCAFFOLDING AND THE PLGA FIBERS PRODUCED ACCORDING TO THIS METHOD
CN105664246A (zh) 一种纳微米多尺度组织工程复合三维支架及其制备方法
CN105727364A (zh) 一种纳微米多尺度聚乳酸三维支架及其制备方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20160706