CN105727268A - Superovulation polyvinylpyrrolidone PMSG composite slow-release injection for sika deer - Google Patents
Superovulation polyvinylpyrrolidone PMSG composite slow-release injection for sika deer Download PDFInfo
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- CN105727268A CN105727268A CN201610106818.0A CN201610106818A CN105727268A CN 105727268 A CN105727268 A CN 105727268A CN 201610106818 A CN201610106818 A CN 201610106818A CN 105727268 A CN105727268 A CN 105727268A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K38/00—Medicinal preparations containing peptides
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- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
- A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
- A61K31/7036—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin having at least one amino group directly attached to the carbocyclic ring, e.g. streptomycin, gentamycin, amikacin, validamycin, fortimicins
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- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0002—Galenical forms characterised by the drug release technique; Application systems commanded by energy
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- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
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- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
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Abstract
The invention discloses a method for preparing polyvinylpyrrolidone PMSG composite superovulation slow-release injection for sika deer. The method comprises the following steps: uniformly dispersing three substances, such as PMSG, acidic xylosidase and gentamicin in polyvinylpyrrolidone, and forming a slow-release agent; and forming a slow-release agent in relatively slow release through intravenous injection of sika deer ear edge, so that target substances and indirectly produced nutrient substances can continuously and uniformly act on ovary cells so as to promote follicle growth and maturity; and therefore, a good effect on superovulation is achieved, and the mature ovum is high in quality. According to the dosage form, stress reaction is reduced by intravenous injection, and intense struggling caused by pain is alleviated.
Description
Technical field
The present invention provides a kind of Cervus nippon Temminck polyvinylpyrrolidone PMSG compound superovulation slow releasing injection, belongs to domestic animal technical field of medicine preparation.
Background technology
Pregnant mare serum gonadotrop(h)in (PMSG) PMSG, classical theory thinks that PMSG is that pregnant mare endometrium cup tissue (placenta materna) produces, and its reason is by lucky and PMSG appearing and subsiding in serum the time consistency that formed and degenerate of this tissue.But having people to prove, PMSG is produced by allantois chorion (fetal placenta) cell.PMSG is a kind of glycoprotein hormones, molecular weight about 53000.PMSG has the double activity of FSH and LH and is widely used on husbandry sector；Promote ovarian follicular growth；Promote the hypertrophy of follicular cell and estrogen synthesis and secretion, stimulate LH receptor on follicle to produce；Jenny, promote maturation and the ovulation of follicle；Stimulate endo cell in follicle to produce estrogen (providing premise material for granular cell synthetic estrogen), the granular cell luteinization after ovulation rate, maintain lutein cell secretion progesterone.PMSG is used to the superovulation of female animal.
Summary of the invention
The present invention provides a kind of Cervus nippon Temminck polyvinylpyrrolidone PMSG compound superovulation slow releasing injection, for the ovulation of Cervus nippon Temminck is carried out super several decorporation.
The preparation method that the present invention further provides a kind of Cervus nippon Temminck polyvinylpyrrolidone PMSG compound superovulation slow releasing injection, it is adaptable to industrialized production.
A kind of Cervus nippon Temminck polyvinylpyrrolidone PMSG compound superovulation slow releasing injection of the present invention, it is characterised in that be made up by ratio of weight and the number of copies of following medicine:
PMSG:1000 ~ 1400 iu；Polyvinylpyrrolidone: 1.0 ~ 1.4 grams；Acid xylose enzyme: 0.6 ~ 0.9 gram；Gentamycin: 6 ~ 100,000 units；Double distilled water: 20 ~ 25 milliliters.
The preparation method of a kind of Cervus nippon Temminck polyvinylpyrrolidone PMSG compound superovulation slow releasing injection of the present invention, comprises the following steps:
Weighing said medicine in proportion, first PMSG and acid xylose enzyme are added polyvinylpyrrolidone, add double distilled water, after fully mixing, then add gentamycin, mix homogeneously makes superovulation slow releasing agent.
Usage and dosage: intravenous injection；Injecting dosage every day is 2.0 ~ 2.4 milliliters.
PMSG, acid xylose enzyme, three kinds of materials of gentamycin are evenly dispersed in polyvinylpyrrolidone by the present invention, form slow releasing agent；nullInjected by Cervus nippon Temminck auricular vein，Form the slow releasing agent of a relative slow release，It discharges PMSG at short notice continuously equably in blood、Acid xylose enzyme、Gentamycin，These materials are made to be transported in each capillary network of health by blood circulation，PMSG can lead to transovarial capillary action on the immature follicle of ovary after arriving ovary，Through continual effect，It makes rapidly follicular cell a large amount of propagation simultaneously of ovary at short notice，The follicle of a large amount of propagation needs to obtain substantial amounts of nutrient substance from residing surrounding and includes sugar，Acid xylose enzyme is applied to digestive system by blood circulation makes the nutritional labeling in food obtain fully digestion entrance blood，The nutrition of abundance is provided to follicle by blood circulation，So as to maturation has had certain guarantee rapidly，Gentamycin serves potent health-care effect around follicle，The final ovulation wound to folliculus ovarii has certain curative effect,These three material continues synergistic result makes the substantial amounts of growth simultaneously of the ovum in the follicle on ovary and ripe gradually.In conjunction with using prostatitis enol to make the lipolyse around follicular cell, having fired mature egg and departed from the purpose of follicular antrum, and reached good superovulation result, prostatitis enol uses according to explanation according to what market was sold.Intravenous injection is compared with intramuscular injection or lumbar injection, and the few pain of teleneuron distribution alleviates, rabbit will not be caused to struggle because having an intense pain and strong disposal stress, the intravenous injection of this polyvinylpyrrolidone slow releasing agent is respond well.
The positive effect of the present invention is in that: the nutrient substance of target substance and generation indirectly can be made to act on from gonad cell uniformly continually, thus promoting follicle to increase and ripe, thus reaching the good result of superovulation, ripe ovum quality is excellent；This dosage form adopts intravenous injection to reduce stress, ease the pain and the fierce struggle that causes.
Detailed description of the invention
Illustrated further the description present invention by following example, and do not limit the present invention in any way, under the premise without departing substantially from the technical solution of the present invention, any change or change that those of ordinary skill in the art made for the present invention easily realize fall within scope of the presently claimed invention.
Embodiment 1
By adding polyvinylpyrrolidone 1.1 grams in the acid xylose enzyme of the PMSG of 1000 ius and 0.6 gram, adding 25 milliliters of double distilled waters, after fully mixing, then add the gentamycin of 80,000 units, mix homogeneously makes superovulation slow releasing agent.Ensure one day 24 hours uniformly to discharge the medicine that this method uses.
Embodiment 2
First the acid xylose enzyme of the PMSG of 1200 ius and 0.7 gram being added polyvinylpyrrolidone 1.0 grams, add 22 milliliters of double distilled waters, use after fully mixing, then add the gentamycin of 70,000 units, mix homogeneously makes superovulation slow release formulation.Ensure one day 24 hours uniformly to discharge the medicine that this method uses.
Embodiment 3
First the acid xylose enzyme of the PMSG of 1400 ius and 0.9 gram being added polyvinylpyrrolidone 1.2 grams, add 23 milliliters of double distilled waters, after fully mixing, then add 60,000 unit gentamycins, mix homogeneously makes superovulation slow releasing agent.Ensure one day 24 hours uniformly to discharge the medicine that this method uses.
Tests below example shows the present invention effect for female Cervus nippon Temminck superovulation:
Test example
1. materials and methods: normal saline, the cotton ball soaked in alcohol of 75%, gentamycin, double distilled water, scalpel, scissors, tweezers, antiseptic gauze, rabbit operating-table, 1,2,5,10 milliliters of syringes, the beaker of 50 milliliters and 10 milliliters, surface plate, continuous zoom stero microscope
2. experimental design: not conceived adult female Cervus nippon Temminck is divided into three groups, and often group 8, respectively adopts the superovulation group of the present invention, well-established law superovulation group and matched group
3. the superovulation of this law experimental group mother Cervus nippon Temminck processes
When testing the 1st ~ 3 day morning 9 ~ 10, being respectively adopted auricular vein and inject superovulation slow releasing agent 2..0 ~ 2.2 milliliter of this law, the same time carries out for three days on end, injects a pin decorporation 3 at the 4th day, at the 5th day to for the female Cervus nippon Temminck anesthesia of examination, dissects；The ovary ovulation point of every Cervus nippon Temminck is counted, is then carried out fimbriae of uterine tube and rushes embryo art, reclaim ovum with sterilizing beaker, be placed on again afterwards in surface plate and pick up ovum under the microscope, finally draw the ovum number of microscopy.
The super superovulation of well-established law: PMSG is directly diluted with double distilled water, to the PMSG of every Cervus nippon Temminck injection with this law moderate, whole super ovulation procedures is ibid；And matched group does not do any superovulation and processes.
Conclusion: shown by above-mentioned 3 groups of embodiment results: (ovary number of eggs ovulated meansigma methods (39,33,41) and microscopy ovum on average detect number (31,21,35) and be substantially better than the super-ovulation effect of PMSG to take the Superovulation of medicine of the present invention, its corresponding data are (11,12,9) and (6,7,5), show that the collaborative compatibility of each material of the present invention can make the nutrient substance of target substance and generation indirectly act on uniformly from gonad cell continually, thus promoting follicle to increase and ripe, thus reaching the good result of superovulation.This dosage form adopts intravenous injection to reduce stress, ease the pain and the fierce struggle that causes.
Claims (2)
1. a Cervus nippon Temminck polyvinylpyrrolidone PMSG compound superovulation slow releasing injection, it is characterised in that be made up by ratio of weight and the number of copies of following medicine:
PMSG:1000 ~ 1400 iu；Polyvinylpyrrolidone: 1.0 ~ 1.4 grams；Acid xylose enzyme: 0.6 ~ 0.9 gram；Gentamycin: 6 ~ 100,000 units；Double distilled water: 20 ~ 25 milliliters.
2. the preparation method of a kind of Cervus nippon Temminck polyvinylpyrrolidone PMSG compound superovulation slow releasing injection according to claim 1, comprises the following steps:
Weighing said medicine in proportion, first PMSG and acid xylose enzyme are added polyvinylpyrrolidone, add double distilled water, after fully mixing, then add gentamycin, mix homogeneously makes superovulation slow releasing agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610106818.0A CN105727268A (en) | 2016-02-28 | 2016-02-28 | Superovulation polyvinylpyrrolidone PMSG composite slow-release injection for sika deer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610106818.0A CN105727268A (en) | 2016-02-28 | 2016-02-28 | Superovulation polyvinylpyrrolidone PMSG composite slow-release injection for sika deer |
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| Publication Number | Publication Date |
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| CN105727268A true CN105727268A (en) | 2016-07-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610106818.0A Pending CN105727268A (en) | 2016-02-28 | 2016-02-28 | Superovulation polyvinylpyrrolidone PMSG composite slow-release injection for sika deer |
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| Country | Link |
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| CN (1) | CN105727268A (en) |
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2016
- 2016-02-28 CN CN201610106818.0A patent/CN105727268A/en active Pending
Non-Patent Citations (5)
| Title |
|---|
| 中国农业科学院饲料研究所编: "《中国饲料原料采购指南 第2版》", 30 November 2007 * |
| 吕津等: "牛羊超数排卵方法研究进展", 《黑龙江动物繁殖》 * |
| 殷玉鹏等: "梅花鹿卵母细胞体外成熟的研究", 《中国畜牧兽医药学会动物解剖学及组织胚胎学分会第十五次学术研讨会论文集》 * |
| 郭洪伟: "天然动物生长促进剂 "速长灵"研制成功", 《农民致富之友》 * |
| 魏海军等: "家养梅花鹿腹腔镜输精技术研究", 《经济动物学报》 * |
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