Isotonic probiotics preparation and preparation method thereof
Technical field
The present invention relates to the isotonic probiotics preparation or solid or solid isotonic probiotics preparation of liquid or liquid and its
Preparation method belongs to health food/dairy products technical field.
Background technique
The existing very long history of application of the probiotics in dairy products, especially in recent years, people confirmed lactic acid bacteria etc.
For beneficial bacteria of intestinal tract, and there are many important physiological functions.Due to the unique flavor of probiotics preparation, nutrition abundant and good
Health-care effect, favored by the majority of consumers.
Traditionally, common probiotics mainly has Bifidobacterium and lactobacillus.With the continuous depth of probio research
Enter, the type of probiotics is just stepped up, and application range is also in further expansion.It can be used for the probiotics strain of food: green
Spring Bifidobacterium, animal bifidobacteria (bifidobacterium lactis), bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, long pair
Discrimination bacillus, lactobacillus acidophilus, Lactobacillus casei, Lactobacillus crispatus, lactobacillus delbruockii subspecies bulgaricus (bulgarian milk bar
Bacterium), Lactobacillus delbrueckii cream subspecies, lactobacillus fermenti, lactobacillus gasseri, Lactobacillus helveticus, Yue Shi lactobacillus, secondary cheese cream bar
Bacterium, lactobacillus plantarum, lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus salivarius, streptococcus thermophilus, Lactococcus lactis
Subspecies, lactococcus lactis subsp and Lactococcus lactis biacetyl subspecies, P. freudenreichii ssp subspecies.
A kind of probiotics active probiotic drink high as unique flavor, nutritive value has whole intestines, stomach invigorating, helps and disappear
Change, can be may be summarized to be with moisturizing, improving eyesight, permanent tooth, strong hair, beauty, effect: 1. prevent and treat diarrhea;2. alleviating lactase deficiency
Shape;3. preventing vagina infection;4. enhancing human immunity;5. Reduce allergy acts on;6. reducing serum cholesterol;7. pre- anti-cancer
With inhibition tumour growth;8. preventing and treating artery sclerosis, disease of cardiovascular system.
In biology and medicine, isotonic is typically all for opposite human body fluid.The osmotic pressure of human body fluid is with dense
Degree is to indicate being between 280-320mol/L.When the osmotic pressure of a solution and the osmotic pressure of body fluid are equal or close, it is exactly
Isotonic solution.In the state of isotonic isobaric, electrolyte particle contained by substance is extremely small, and unit is only and one blood of human body
Five one thousandths of ball, it cracking can enter and merge intracorporal cell and tissue, therefore can quickly be inhaled by human body cell
It receives and utilizes, the formula of isotonic equipressure guarantees that human body quickly absorbs nutriment maximum.
Currently, the main profitable probliotics acidified milk of probiotics preparation, lactic acid bacteria acidic milk drink, probiotics powder agent in the market
Deng.Nutriment in common probiotics preparation is oral into stomach, and by peptic digest, the institute of small intestinal absorption is nutritious all necessary
Into portal vein to liver, after being handled by liver, it is published to whole body, many nutrition are transported after being utilized by liver, and nutritional ingredient is by liver
Modification, transformation, concentration reduce.
Nutriment in isotonic probiotics preparation quickly immediately begins to inhale much with molecular forms from the above mucous membrane of stomach
It receives, into blood circulation, participates in cell metabolism.Nutrition rapidly enters circulation, preferably plays the function and effect of probiotics, mentions
The high absorption of probiotics preparation nutriment, be really achieved can simultaneously, sufficiently, balance and the function of nutriment and moisture in time
Effect.
Summary of the invention
The object of the present invention is to provide liquid or liquid or solid or solid isotonic probiotics preparation.Preparation is also provided
Their method: liquid form or liquid or solid form or solid probiotic milk base-material are mixed with nutrition fortifier
Afterwards, then with Sweet flavor modifier, isotonic regulator and other auxiliary materials it is uniformly mixed to get the isotonic probiotics preparation.At this
Isotonic adjusting host agent and the isotonic adjusting auxiliary agent of weakly acidic E) are used in combination in application.
The osmotic pressure of liquid or the isotonic probiotics preparation of liquid in 250~340mol/L, preferably 270~320mol/L,
More preferable 285~305mol/L.Additionally, it is preferred that the pH value of liquid or the isotonic probiotics preparation of liquid is 6.0~6.8, preferably
6.2~6.6, more preferable 6.3~6.5.
Solid or solid isotonic probiotics preparation with 2.5-5.5 times, preferably 3-5 times, it is 3.5-4.5 times more preferable
The osmotic pressure of the isotonic probiotics preparation of water dilution, the isotonic probiotics preparation of acquisition liquid or liquid, the liquid or liquid exists
250~340mol/L, preferably 270~320mol/L, more preferable 285~305mol/L.Additionally, it is preferred that the liquid or liquid
The pH value of isotonic probiotics preparation is 6.0~6.8, preferably 6.2~6.6, more preferable 6.3~6.5.
Therefore, according to the first embodiment of the present invention, the isotonic probiotics preparation of a kind of liquid or liquid is provided, it
Include: A1) liquid probiotic bacterium cow's milk base-material, B) nutrition fortifier, C) Sweet flavor modifier, D) isotonic adjusting host agent and E) isotonic tune
Saving auxiliary agent, it is characterised in that: A1) liquid probiotic bacterium cow's milk base-material is after being fermented by cow's milk with live probiotic bacterium leavening agent
Fermenation raw liquid obtained, D) isotonic adjusting host agent is glucose and/or fructose and E) isotonic adjustings auxiliary agent is to be selected from lemon
One of acid, malic acid, lactic acid, tartaric acid, vitamin C and sodium tripolyphosphate are a variety of.
Preferably, the dosage of each component is:
A1) liquid probiotic bacterium cow's milk base-material: 100 parts by weight
B) nutrition fortifier: 0.03~10 parts by weight, preferably 0.05~8 parts by weight, preferably 0.08~6 parts by weight, preferably
0.1~5 parts by weight,
C) Sweet flavor modifier: 0 or 0.5~8 parts by weight, preferably 1.0~7 parts by weight, preferably 1.5~6 parts by weight, preferably
2.0~5 parts by weight,
D) isotonic adjusting host agent: 0.7~10 parts by weight, preferably 1.0~8 parts by weight, preferably 1.2~6 parts by weight, preferably
1.5~5 parts by weight,
E) isotonic adjusting auxiliary agent: 0.5~8 parts by weight, preferably 0.8~7 parts by weight, preferably 1.0~6 parts by weight, preferably 1.5
~4 parts by weight.
Further preferably, D) isotonic adjust host agent and E) the isotonic content for adjusting both auxiliary agents or dosage make it is isotonic prebiotic
The osmotic pressure of bacteria preparation is 250~340mol/L, preferably 270~320mol/L, more preferable 285~305mol/L.It is preferred that E) etc.
It seeps the content for adjusting auxiliary agent or dosage makes the pH value of isotonic probiotics preparation be 6.0~6.8, preferably 6.2~6.6, more preferably
6.3~6.5.
According to the second embodiment of the present invention, a kind of solid or solid isotonic probiotics preparation are provided, it includes:
A2) solid or solid-state probiotic milk base-material, B) nutrition fortifier, C) Sweet flavor modifier, D) isotonic adjusting host agent and E) it is isotonic
Adjusting auxiliary agent, it is characterised in that: A2) solid or solid-state probiotic milk base-material be to be carried out by cow's milk with live probiotic bacterium leavening agent
The solids for obtaining fermenation raw liquid after fermentation and then fermenation raw liquid being lyophilized or filtered or is centrifugated and is obtained, D)
Isotonic adjusting host agent is glucose and/or fructose and E) isotonic adjustings auxiliary agent is to be selected from citric acid, malic acid, lactic acid, winestone
One of acid, vitamin C and sodium tripolyphosphate are a variety of.
It is preferred that the dosage of each component is:
A2) solid or solid-state probiotic milk base-material: 5~25 parts by weight, preferably 8~22 parts by weight;It is preferred that 10~20 weights
Measure part;
B) nutrition fortifier: 0.03~10 parts by weight, preferably 0.05~8 parts by weight, preferably 0.08~6 parts by weight, preferably
0.1~5 parts by weight,
C) Sweet flavor modifier: 0 or 0.5~8 parts by weight, preferably 1.0~7 parts by weight, preferably 1.5~6 parts by weight, preferably
2.0~5 parts by weight,
D) isotonic adjusting host agent: 0.7~10 parts by weight, preferably 1.0~8 parts by weight, preferably 1.2~6 parts by weight, preferably
1.5~5 parts by weight,
E) isotonic adjusting auxiliary agent: 0.5~8 parts by weight, preferably 0.8~7 parts by weight, preferably 1.0~6 parts by weight, preferably 1.5
~4 parts by weight.
In above-mentioned first and second embodiment, cow's milk is any one of raw milk, skimmed milk and reconstituted milk.
When using raw milk as above-described milk, need to sterilize in advance.
It is preferred that nutrition fortifier is selected from xylo-oligosaccharide, soybean polyoses, synanthrin, vitamin C, vitamin K, the dimension life of B race
In element, D family vitamin or nutrient for plants (such as catechol, poly- phenol, anthocyanidin or procyanidine) or calcium carbonate or potassium carbonate
It is one or more.
It is preferred that live probiotic bacterium leavening agent is selected from lactobacillus plantarum, Lactobacillus casei, lactobacillus acidophilus, De Shi cream
Bacillus subspecies bulgaricus (i.e. lactobacillus bulgaricus), Lactobacillus delbrueckii cream subspecies, bifidobacterium adolescentis, bifidobacterium lactis,
Bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum, Lactobacillus crispatus, lactobacillus fermenti, grignard cream
Bacillus, Lactobacillus helveticus, Yue Shi lactobacillus, lactobacillus paracasei, lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus salivarius,
Lactococcus lactis subsp. lactis, lactococcus lactis subsp, Lactococcus lactis biacetyl subspecies, P. freudenreichii ssp are sub-
One of kind, streptococcus thermophilus are a variety of.
In above-mentioned first and second embodiment, preparation further include: F) emulsifier.
In above-mentioned first and second embodiment, preparation further include: G) thickener;With, optional (i.e. not essential),
H) edible essence.
In this application, " optional " indicate to be with or without.
According to the third embodiment the present invention, a kind of solid or solid isotonic probiotics preparation are provided, it be by
Preparation according to the first embodiment of the present invention is obtained by being lyophilized.
4th embodiment according to the present invention, provides a kind of system prepared according to the first embodiment of the present invention
The method of agent, this method comprises: will include A1) liquid probiotic bacterium cow's milk base-material, B) nutrition fortifier, C) Sweet flavor modifier, D)
It is isotonic to adjust host agent and E) isotonic various raw materials adjusted including auxiliary agent mix and obtain liquid or liquefied mixture.
Preferably, in the above preparation method, the osmotic pressure and pH value of liquid or liquefied mixture obtained are measured,
Further gradually add D) isotonic adjusting host agent and E) isotonic adjusting auxiliary agent, so that the pH value of final isotonic probiotics preparation is
6.0~6.8, preferably 6.2~6.6, more preferable 6.3~6.5, meanwhile, so that the osmotic pressure of isotonic probiotics preparation be 250~
340mol/L, preferably 270~320mol/L, more preferable 285~305mol/L.
Sweet flavor modifier be selected from white granulated sugar, brown sugar, sucrose, fructose syrup, beet sugar, maltose, fructose, lactose,
One of glycitols sweetener, Sucralose, acesulfame potassium and Aspartame are a variety of.
Solid or solid isotonic probiotics preparation can be the form of granule or tablet (or tabletting).
It is preferred that thickener is in pectin, carragheen, xanthan gum, sodium alginate, sodium carboxymethylcellulose and microcrystalline cellulose
It is one or more.
It is preferred that emulsifier is glycerin monostearate and/or sucrose ester.
The dosage of thickener is such as 0.1~5.0wt%.The dosage of the emulsifier is 0.05~0.8wt%.It is described
Edible essence dosage be 0.03~1.0wt%.Here percentage is all based on the total weight of preparation.
The isotonic probiotics preparation of liquid of the present invention is transferred to 2~10 DEG C of freezers after filling, refrigeration.
The isotonic probiotics preparation of solid of the present invention room temperature shady place after packing saves.
Compared with prior art, the present invention has following advantageous effects:
1, pass through D) isotonic adjust host agent and weakly acidic E) it is isotonic adjust being used in combination for auxiliary agent, be capable of providing isotonic
Effect, more particularly to eliminate other auxiliary additives for isotonic influence or interference, it is ensured that the isoosmotic pressure of preparation is in ideal
In the range of.Meanwhile it is also to be ensured that the pH value of preparation or system within the scope of pH optimal for active probiotic.If
Isoosmotic pressure controls except 250~340mol/L range, then leads to infiltration rate or absorption when being lower than the lower limit value of the range
Rate decline, and also digestive system is dehydrated when being higher than the upper limit value of the range, it influences to absorb in turn.Weakly acidic E)
In addition isotonic adjusting auxiliary agent also plays a part of pH buffer.
2, it is suitble to Human Physiology requirement
The present invention is conducive to the nutriment in isotonic probiotics preparation by the way that isotonic probiotics preparation is made in probiotics
It quickly absorbs, rapidly enters circulation, be suitble to Human Physiology requirement, preferably played the function and effect of probiotics preparation, very
Just reaching can simultaneously, sufficiently, balance and nutriment and the effect of moisture in time.The isotonic probiotics preparation that the present invention makes compared with
Solves this technical problem well, product tissue is in good condition, system is good to thermostabilization, taste flavor.
3, prebiotic bacterial content is high
In the application, pass through D) isotonic adjust host agent and weakly acidic E) it is isotonic adjust being used in combination for auxiliary agent, compared with
The long time (such as solid pharmaceutical preparation, 10 months, being up to 20 days for liquid preparation) maintains the activity of probiotics.For example,
If adding probiotics fermention stoste, the prebiotic bacterial content of isotonic probiotics preparation obtained in the formulation with the ratio of 20wt%
Up to 1.0 × 109Cfu/g, and use glycerol and/or D-sorbite as isotonic agent in the comparative example, it is obtained prebiotic
Prebiotic bacterial content is mostly 3 × 10 in bacteria preparation8Cfu/g or less.
In the solid pharmaceutical preparation that the application obtains, exist in manufactured probiotic tablet in 0~3 month number of live bacteria of probiotics
1010Cfu/g or more, the 4th~5 month number of live bacteria of probiotics is 109Cfu/g or more existed in 6~9 months number of live bacteria of probiotics
108Cfu/g or more, in 10~12 months number of live bacteria of probiotics 107Cfu/g or more.
4, probiotics stable content
Existing probiotics dairy products are mostly acidified milk or fermentation milk beverage, since product system is acid (pH is lower than 6.0),
It is unfavorable for the survival of probiotics, under refrigerated conditions, in 30 day shelf-life, bacterium number content is unstable, there is 5~10 times of reduction;
But be conducive to the survival of probiotics close under neutrallty condition in this application, under refrigerated conditions, in 30 day shelf-life, bacterium
Number content is substantially unchanged.
5, it is rich in probiotics fermention metabolic physiology active material
Comprising the cow's milk after probiotics fermention in preparation of the invention, it is rich in probiotics metabolic physiology active material, it is right
For some new type functional probiotics, certain metabolites play an important role.As Lactobacillus casei fermentation cow's milk generates
Inhibiting peptide of tonin section, γ-aminobutyric acid and exocellular polysaccharide there is the blood pressure effect that adjusts, and it is existing prebiotic
Bacterium cow's milk is direct addition probiotics bacterial powder, wherein not containing probiotics fermention metabolic physiology active material.
6, fortification
Nutrition fortifier used in the present invention provides more nutrition for human body normal need.
In short, the present invention provides a kind of product formulas different from original beverage, by ingredient type and content
Adjustment, adds nutrition fortifier appropriate, meets the isotonic feature of beverage, realizes the nutrition for effectively supplementing needed by human body really
Element reaches to the balanced nutritious of human body, and realization product mouthfeel is harmonious with osmotic pressure characteristic.
Detailed description of the invention
Fig. 1 is the process flow chart of the isotonic probiotics preparation of liquid (isotonic probiotics liquid beverage) of embodiment 1.
Fig. 2 is the process flow chart of the isotonic probiotics preparation of solid (isotonic probiotics tablets) of embodiment 4.
Fig. 3 is the process flow chart of the isotonic probiotics preparation of solid (isotonic probiotic granulate) of embodiment 5.
Specific embodiment
The present invention is further illustrated below by the mode of embodiment, but does not therefore limit the present invention to the reality
It applies among a range.The preparation method of actual conditions is not specified in the following example, according to conventional methods and conditions, or according to saying
Bright book selection.
Embodiment 1
1) prepare liquid probiotic milk base-material (i.e. liquid probiotic bacterium base-material): double centner skimmed milk is sterilized through 96 DEG C
90min accesses 3.0 grams of strain of lactobacillus plantarum (LP28) after being cooled to 37 DEG C, 2.0 grams of lactobacillus paracasei strain, heat preservation is sent out
Ferment is made liquid probiotic bacterium cow's milk base-material (i.e. liquid probiotic bacterium base-material) to 230 ° of T of titratable acidity;
2) nutrition fortifier adds: by liquid probiotic bacterium base-material double centner and 0.05 kilogram of xylo-oligosaccharide, 0.05 kilogram greatly
The mixing of beans polysaccharide, 0.04 kilogram of grade sterility calcium carbonate, and stir evenly;
3) Sweet flavor modifier is added: 1.5 kilograms of white sugar, 1.5 kilograms of fructose syrups, 0.07 kilogram of acesulfame potassium, 0.08 are added
Kilogram A Basi sweet tea, and stir evenly;
4) 0.5 kilogram of citric acid, 0.5 kilogram of malic acid, 0.5 kilogram of tripolyphosphate the isotonic addition for adjusting auxiliary agent: is added
Sodium, and stir evenly;Measuring pH value is 6.4.
5) the isotonic addition for adjusting host agent: the osmotic pressure of mixture of the measurement containing probiotics adds grape according to osmotic pressure
About 1.5 kilograms of stirrings of sugar, until being uniformly mixed, detection Liquid Penetrant presses 290mol/L.
6) other auxiliary material additions: 0.5 kilogram of edible essence and 0.5 kilogram of emulsifier glycerin monostearate is added and stirs
It mixes uniformly;Measure Liquid Penetrant pressure 295mol/L again to get the isotonic probiotics preparation of liquid form;
Product is filling and refrigerates:
Isotonic probiotics preparation is filling to packing container, and it is transferred to 5 DEG C of freezers rapidly, it refrigerates.
Product process flow is shown in Fig. 1.
Embodiment 2
Prepare liquid probiotic milk base-material (i.e. liquid probiotic bacterium base-material): double centner skimmed milk is sterilized through 96 DEG C
90min, accesses 5.0 grams of lactobacillus paracasei strain after being cooled to 37 DEG C, liquid is made in heat-preservation fermentation to 230 ° of T of titratable acidity
Probiotics base-material;
Nutrition fortifier addition: liquid probiotic bacterium base-material double centner and 0.3 kilogram of concentrated strawberry juice, 0.5 kilogram of dimension are given birth to
Plain D3,0.5 kilogram of vitamin B1 and 0.03 kilogram of grade sterility calcium carbonate, mixing, and stir evenly;
Sweet flavor modifier addition: 1.5 kilograms of white sugar, 1.5 kilograms of fructose are added, and stir evenly;
The isotonic addition for adjusting auxiliary agent: 1.0 kilograms of vitamin Cs are added, and stir evenly;Measuring pH value is 6.6.
Isotonic to adjust host agent addition: the osmotic pressure of mixture of the measurement containing probiotics adds glucose about according to osmotic pressure
1.5 kilograms of stirrings, until being uniformly mixed, detection Liquid Penetrant presses 292mol/L,
Other auxiliary material additions: 0.5 kilogram of edible essence is added, and stirs evenly;Liquid Penetrant pressure 296mol/ is measured again
L is to get isotonic probiotics preparation;
Product is filling and refrigeration: isotonic probiotics preparation is filling to packing container, and it is transferred to 5 DEG C of freezers rapidly, it refrigerates.
Embodiment 3
Prepare liquid probiotic milk base-material (i.e. liquid probiotic bacterium base-material): double centner skimmed milk is sterilized through 96 DEG C
90min, accesses 5.0 grams of lactobacillus paracasei strain after being cooled to 37 DEG C, liquid is made in heat-preservation fermentation to 230 ° of T of titratable acidity
Probiotics base-material;
Nutrition fortifier addition: liquid probiotic bacterium base-material double centner and 0.3 kilogram of concentrated strawberry juice, 0.5 kilogram of dimension are given birth to
Plain D3, vitamin B1, the mixing of 0.02 kilogram of grade sterility calcium carbonate, and stir evenly;
Sweet flavor modifier addition: 1.5 kilograms of white sugar, 1.5 kilograms of fructose are added, and stir evenly;
The isotonic addition for adjusting auxiliary agent: 1.0 kilograms of vitamin Cs are added, and stir evenly;Measuring pH value is 6.5.
Isotonic to adjust host agent addition: the osmotic pressure of mixture of the measurement containing probiotics adds glucose about according to osmotic pressure
1.5 kilograms of stirrings, until being uniformly mixed, detection Liquid Penetrant presses 292mol/L,
Other auxiliary material additions: 0.5 kilogram of edible essence is added, and stirs evenly;Up to isotonic probiotics preparation;
Product is filling and refrigeration: isotonic probiotics preparation is filling to packing container, and it is transferred to 5 DEG C of freezers rapidly, it refrigerates.
Embodiment 4
It prepares solid or solid-state probiotic milk base-material (i.e. solid probiotics base-material): liquid probiotic is made in embodiment 1
Bacterium base-material is filtered, is freeze-dried obtained solid probiotics base-material;
Nutrition fortifier addition: by 10 kilograms of solid probiotics base-material and 1.0 kilograms of grade sterility calcium carbonate, 0.5 thousand
Gram vitamine D3,0.5 kilogram of vitamin B1 mixing, and be uniformly mixed;
Sweet flavor modifier addition: 0.15 kilogram of A Basi sweet tea is added, and is uniformly mixed;
The isotonic addition for adjusting auxiliary agent: 1.0 kilograms of citric acids, 0.5 kilogram of malic acid are added, and are uniformly mixed;
Isotonic adjusting host agent addition: 1.5 kilograms of glucose are added, and is uniformly mixed:
Gained mixture carries out tabletting: through granulation, whole grain, dry conforming particle, tabletting;
The measurement of osmotic pressure: taking isotonic probiotics tablets 3g, be dissolved in 100ml water, and detection Liquid Penetrant pressure is 295mol/L,
Up to the isotonic probiotics preparation of solid (isotonic probiotics tablets);
Product packing and preservation: the isotonic probiotics preparation of solid is dispensed to packing container, sets shady place storage.
Product process flow is shown in Fig. 2.
Embodiment 5
It prepares solid or solid-state probiotic milk base-material (i.e. solid probiotics base-material): liquid probiotic is made in embodiment 1
Bacterium base-material is filtered, is freeze-dried obtained solid probiotics base-material;
Nutrition fortifier addition: by 10 kilograms of solid probiotics base-material and 1.0 kilograms of grade sterility calcium carbonate, 0.5 thousand
Gram vitamine D3,0.5 kilogram of vitamin B1 mixing, and be uniformly mixed;
Sweet flavor modifier addition: 0.15 kilogram of A Basi sweet tea is added, and is uniformly mixed;
The isotonic addition for adjusting auxiliary agent: 1.0 kilograms of citric acids, 0.5 kilogram of malic acid are added, and are uniformly mixed;
Isotonic adjusting host agent addition: 1.5 kilograms of glucose are added, and is uniformly mixed:
Other auxiliary material additions: 0.5 kilogram of dextrin is added, and is uniformly mixed;
Gained mixture carries out tabletting: through granulation, whole grain, dry conforming particle, tabletting;
The measurement of osmotic pressure: taking isotonic probiotic granulate 3g, be dissolved in 100ml water, and detection Liquid Penetrant pressure is 295mol/
L is to get the isotonic probiotics preparation of solid (isotonic probiotics tablets);
Product packing and preservation: the isotonic probiotics preparation of solid is dispensed to packing container, sets shady place storage.
Product process flow is shown in Fig. 3.
Embodiment 6
Prepare liquid probiotics base-material: double centner skimmed milk is accessed after being cooled to 37 DEG C and is planted through 96 DEG C of sterilization 90min
3.0 grams of object lactobacillus strain, 2.0 grams of lactobacillus paracasei strain, liquid probiotic is made in heat-preservation fermentation to 230 ° of T of titratable acidity
Bacterium base-material;
Nutrition fortifier addition: by liquid probiotic bacterium base-material double centner and 0.05 kilogram of xylo-oligosaccharide, 0.05 kilogram of soybean
Polysaccharide mixing, and stir evenly;
Sweet flavor modifier addition: 1.5 kilograms of white sugar, 1.5 kilograms of fructose syrups, 0.07 kilogram of acesulfame potassium, 0.08 thousand are added
Ke Abasi sweet tea, and stir evenly;
The isotonic addition for adjusting auxiliary agent: being added 0.5 kilogram of citric acid, 0.5 kilogram of malic acid, 0.5 kilogram of sodium tripolyphosphate,
And it stirs evenly;Measuring pH value is 6.4.
The isotonic addition for adjusting host agent: the osmotic pressure of mixture of the measurement containing probiotics adds glucose according to osmotic pressure
About 1.5 kilograms of stirrings, until being uniformly mixed, detection Liquid Penetrant presses 290mol/L.
Other auxiliary material additions: 0.5 kilogram of edible essence of addition, 0.3 kilogram of potassium sorbate, and stir evenly;It measures again
Liquid Penetrant presses 295mol/L to get isotonic probiotics preparation;
Product is filling and refrigerates: the isotonic probiotics preparation of liquid is filling to packing container, set shady place storage.
Embodiment 7
Embodiment 1 is repeated, the isotonic probiotics preparation of the liquid form obtained of embodiment 1 is lyophilized, obtains solid shape
The isotonic probiotics preparation of formula.
Comparative example 1
Embodiment 1 is repeated, only using 1.5 kilograms of glycerol (glycerine) and 1.5 kilograms of D-sorbite as isotonic
Regulator replaces isotonic adjusting host agent and isotonic adjusting auxiliary agent used in embodiment 1.In the isotonic adjusting host agent of step 5)
After addition, detect the osmotic pressure 298mol/L of liquid, but, step 6) add other auxiliary materials (i.e. 0.5 kilogram of edible essence,
With 0.5 kilogram of emulsifier glycerin monostearate and stir evenly) after measure again liquid osmotic pressure be 355mol/L, this
Illustrate that the addition of auxiliary material has influenced the osmotic pressure of preparation.The pH value of gained preparation is 5.8 in step 6).
Comparative example 2
Comparative example 1 is repeated, only liquid preparation is lyophilized.
Table 1- probiotics stable content
Table 2- probiotics stable content