CN105724254B - A kind of Fraxinus velutina excised leaf body embryo inducing culture - Google Patents
A kind of Fraxinus velutina excised leaf body embryo inducing culture Download PDFInfo
- Publication number
- CN105724254B CN105724254B CN201610163915.3A CN201610163915A CN105724254B CN 105724254 B CN105724254 B CN 105724254B CN 201610163915 A CN201610163915 A CN 201610163915A CN 105724254 B CN105724254 B CN 105724254B
- Authority
- CN
- China
- Prior art keywords
- body embryo
- fraxinus velutina
- excised leaf
- inducing culture
- leaf body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention discloses a kind of culture medium that somatic embryo is induced from Fraxinus velutina excised leaf.The formula is MS salinities+B5Organic+1.5mgL–16 benzyladenines (BA)+4.0mgL–1Methyl α-naphthyl acetate (NAA)+0.5mgL–12,4 dichlorphenoxyacetic acids (2,4 D)+50.0gL–1Sucrose+5.0gL–1Agar, pH value 5.2-5.4.Body embryo inductivity is high, is adapted to the white industrial seedling rearing production of fine hair.
Description
Technical field
Go out the present invention relates to a kind of body embryo inducing culture, more particularly to one kind from Fraxinus velutina embryonic callus induction
The culture medium of somatic embryo, i.e. Fraxinus velutina excised leaf body embryo inducing culture；Belong to Chinese wax tissue culture technique.
Background technology
Fraxinus velutina (Fraxinus velutina Torr.) is that Oleaceae (oleaceae) Fraxinus (Fraxinus) falls
Leaf arbor, is the important reproducting tree species of northern China, is especially important Salt And Alkali Tolerance arbor species in varieties in saline-alkali areas, Salt And Alkali Tolerance,
Fighting drought, barren-resistant, strong stress resistance, wide adaptation range, and with attractive in appearance, fast-growing, material superior properties, in afforestation, use material
Extensive use in woods, shelterbelt construction.In recent years, Fraxinus velutina plant tissue culture technique system, Chen Zhiqun are tentatively established
Deng (2006) using Fraxinus velutina stem section as explant, regeneration plant is obtained by shoot proliferation approach；Wang Lei etc. (2008) is with suede
Hair Chinese wax mature embryo and stem section be explant, have studied its tissue cultures and plant regeneration technique, average coefficient of proliferation is 3.59,
In the presence of breeding, proliferation rate is low and the low defect of rooting rate.So far, about in all kinds of documents of plant tissue culture technique, still
Have no the research report induced on Fraxinus velutina body embryo.
Body embryo induced tissue culture technique system is not only the optimal receptor system of transgenosis, is also seedling fast breeding
One of effective ways.A kind of culture medium for going out somatic embryo from Fraxinus velutina embryonic callus induction of exploitation, i.e. fine hair is white
Wax excised leaf body embryo inducing culture is expected to be that fast breeding and the initiative excellent new germ plasm of Fraxinus velutina have put forward new way.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide a kind of training of Fraxinus velutina excised leaf body embryo induction
Base is supported, production and new germ plasm innovative research are bred on a large scale for Fraxinus velutina elite germplasm.
Fraxinus velutina excised leaf body embryo inducing culture of the present invention, is by 6-benzyladenine (6-BA), naphthalene
Acetic acid (NAA), 2,4- dichlorphenoxyacetic acids (2,4-D), sucrose and agar, are dissolved in MS salinities+B5In organic minimal medium
It is made, its pH value is 5.2-5.4；It is characterized in that：
The amounts of components of the Fraxinus velutina excised leaf body embryo inducing culture is with the addition of the organic minimal mediums of 1L
Amount is calculated as：
Wherein, above-mentioned MS salinities+B5The component of organic minimal medium is：
KNO3 1900mg·L-1, NH4NO3 1650mg·L-1, MgSO4·7H2O 370mg·L-1, KH2PO4 170mg·
L-1, CaCl2·2H20 440mg·L-1, MnSO4·4H2O 22.3mg·L-1, ZnSO4·7H2O 8.6mg·L-1,
H3BO36.2mg·L-1, KI 0.83mgL-1, NaMoO4·2H2O 0.25mg·L-1, CuSO4·5H2O 0.025mg·L-1,
CoCl2·6H2O 0.025mg·L-1, Na2-EDTA 37.3mg·L-1, FeSO4·7H20 27.8mg·L-1, vitamin
B110mg·L-1, vitamin B61mg·L-1, folic acid 0.5mgL-1, inositol 100mgL-1, glycine 2.0mgL-1。
Above-mentioned Fraxinus velutina excised leaf body embryo inducing culture is preferred embodiment：The Fraxinus velutina is in vitro
The amounts of components of blade body embryonal induction culture medium is calculated as with the addition of the organic minimal mediums of 1L：
In Fraxinus velutina excised leaf body embryo inducing culture disclosed by the invention, MS salinities+B5It is organic to be cultivated to be basic
Base adds plant growth regulator 6-benzyladenine there is provided inorganic needed for plant tissue growth and organic nutrition element
(BA), methyl α-naphthyl acetate (NAA) and 2,4- dichlorphenoxyacetic acid (2,4-D) promote to form the effect of body embryo；Add the sucrose of high concentration
The carbon nutrition of developing tissue growth is provided；Addition agar is solidification culture medium, is played a supportive role.
The consumption proportion of above-mentioned Fraxinus velutina excised leaf body embryo inducing culture each component is that inventor is largely tried
Test and grope to summarize what is drawn, experiment is confirmed：The culture medium has preferable inducing effect, in vitro using basal culture medium Fraxinus velutina
The inductivity of blade body embryo can reach 79%, is adapted to the white industrial seedling rearing production of fine hair, is expected to as fast breeding and initiative fine hair
The excellent new germ plasm of Chinese wax provides new way.
Brief description of the drawings
Fig. 1 blades.
Fig. 2 body embryos and body embryo sprout seedling.
Embodiment
Present invention is further elucidated with reference to embodiment：
Embodiment 1：
The amounts of components of Fraxinus velutina excised leaf body embryo inducing culture is with the addition gauge of the organic minimal mediums of 1L
For：
By above-mentioned 6-benzyladenine (6-BA), methyl α-naphthyl acetate (NAA), 2,4- dichlorphenoxyacetic acids (2,4-D), sucrose and fine jade
Fat, is dissolved in MS salinities+B5Fraxinus velutina excised leaf body embryo inducing culture, its pH value are made in organic minimal medium
For 5.2-5.4.
Embodiment 2：
The amounts of components of Fraxinus velutina excised leaf body embryo inducing culture is with the addition gauge of the organic minimal mediums of 1L
For：
By above-mentioned 6-benzyladenine (6-BA), methyl α-naphthyl acetate (NAA), 2,4- dichlorphenoxyacetic acids (2,4-D), sucrose and fine jade
Fat, is dissolved in MS salinities+B5Fraxinus velutina excised leaf body embryo inducing culture, its pH value are made in organic minimal medium
For 5.2-5.4.
Embodiment 3：
The amounts of components of Fraxinus velutina excised leaf body embryo inducing culture is with the addition gauge of the organic minimal mediums of 1L
For：
By above-mentioned 6-benzyladenine (6-BA), methyl α-naphthyl acetate (NAA), 2,4- dichlorphenoxyacetic acids (2,4-D), sucrose and fine jade
Fat, is dissolved in MS salinities+B5Fraxinus velutina excised leaf body embryo inducing culture, its pH value are made in organic minimal medium
For 5.2-5.4.
In above-described embodiment 1-3, MS salinities+B5The component of organic minimal medium is：
KNO3 1900mg·L-1, NH4NO3 1650mg·L-1, MgSO4·7H2O 370mg·L-1, KH2PO4 170mg·
L-1, CaCl2·2H20 440mg·L-1, MnSO4·4H2O 22.3mg·L-1, ZnSO4·7H2O 8.6mg·L-1,
H3BO36.2mg·L-1, KI 0.83mgL-1, NaMoO4·2H2O 0.25mg·L-1, CuSO4·5H2O 0.025mg·L-1,
CoCl2·6H2O 0.025mg·L-1, Na2-EDTA37.3mg·L-1, FeSO4·7H20 27.8mg·L-1, vitamin
B110mg·L-1, vitamin B61mg·L-1, folic acid 0.5mgL-1, inositol 100mgL-1, glycine 2.0mgL-1。
Embodiment 4：Fraxinus velutina excised leaf body embryo inducing culture compliance test result
Test method and result：
Test material：The in vitro cuttings Fraxinus velutina cultivated with Shandong Forest Science Academy's Forest Tree Genetic Breeding
Blade is used as explant donor.
Blade processing：Blade is cut from base portion, 2-3 wound is cut on each blade, it is paraxial be placed in up it is above-mentioned
Described in embodiment 1-3 on 3 kinds of body embryo inducing cultures, 6-10 piece blades are put into each culture dish (diameter 60mm).
Condition of culture：Light culture, 25 ± 2 DEG C of daytime temperature, 18 ± 2 DEG C of night temperature.
After culture 20 days, most Fraxinus velutina excised leafs generate body embryo, and being now transferred to body embryo germination medium is
MS salinities+B5Organic+30gL–1Glucose+5.0gL–1Agar, pH value 5.8, be transferred to illumination cultivation (25 ± 2 DEG C of daytime temperature,
18 ± 2 DEG C of night temperature, 14 hours illumination/10 hour dark, optical density is 100-120 μm of olm-2·s-1), after cultivating 4 weeks,
Statistical result is as follows：
Interpretation of result is drawn the following conclusions in table：
Body embryo inductivity in embodiment 2 is up to 78%；The body embryo inductivity of embodiment 1 and embodiment 3 is respectively
42% and 56%.
In summary analyze, show that Fraxinus velutina excised leaf body embryo inducing culture is preferred embodiment：It is described
The amounts of components of Fraxinus velutina excised leaf body embryo inducing culture is calculated as with the addition of the organic minimal mediums of 1L：
Claims (1)
1. a kind of Fraxinus velutina excised leaf body embryo inducing culture, be by 6-benzyladenine (6-BA), methyl α-naphthyl acetate (NAA),
2,4- dichlorphenoxyacetic acids (2,4-D), sucrose and agar, are dissolved in MS salinities+B5It is made in organic minimal medium, its pH value
For 5.2-5.4；It is characterized in that：
The amounts of components of the Fraxinus velutina excised leaf body embryo inducing culture is with the addition gauge of the organic minimal mediums of 1L
For：
Wherein, above-mentioned MS salinities+B5The component of organic minimal medium is：
KNO3 1900mg·L-1, NH4NO3 1650mg·L-1, MgSO4·7H2O 370mg·L-1, KH2PO4 170mg·L-1,
CaCl2·2H2O 440mg·L-1, MnSO4·4H2O 22.3mg·L-1, ZnSO4·7H2O 8.6mg·L-1, H3BO36.2mg·
L-1, KI 0.83mgL-1, NaMoO4·2H2O 0.25mg·L-1, CuSO4·5H2O 0.025mg·L-1, CoCl2·6H2O
0.025mg·L-1, Na2-EDTA 37.3mg·L-1, FeSO4·7H20 27.8mg·L-1, vitamin B110mg·L-1, dimension life
Plain B61mg·L-1, folic acid 0.5mgL-1, inositol 100mgL-1, glycine 2.0mgL-1。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610163915.3A CN105724254B (en) | 2016-03-22 | 2016-03-22 | A kind of Fraxinus velutina excised leaf body embryo inducing culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610163915.3A CN105724254B (en) | 2016-03-22 | 2016-03-22 | A kind of Fraxinus velutina excised leaf body embryo inducing culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105724254A CN105724254A (en) | 2016-07-06 |
| CN105724254B true CN105724254B (en) | 2017-10-27 |
Family
ID=56251124
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610163915.3A Active CN105724254B (en) | 2016-03-22 | 2016-03-22 | A kind of Fraxinus velutina excised leaf body embryo inducing culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105724254B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116369203B (en) * | 2023-03-20 | 2024-03-15 | 江苏省中国科学院植物研究所 | Lycoris plant floret regeneration medium and floret regeneration method |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102124954B (en) * | 2011-01-31 | 2012-11-28 | 江苏农林职业技术学院 | Induced rapid propagation culture medium for somatic embryos of leaves in vitro of photinia x frasery |
| CN102228001B (en) * | 2011-04-28 | 2013-01-02 | 东北林业大学 | Micropropagation method of fraxinus rhynchophylla |
| CN102948369A (en) * | 2012-11-28 | 2013-03-06 | 东北林业大学 | Method for improving inductivity of ashtree somatic embryo |
| CN104381128B (en) * | 2014-10-15 | 2016-03-02 | 山东省林业科学研究院 | A kind of method improving the Fraxinus velutina tissue-culturing rapid propagation rate of increase |
-
2016
- 2016-03-22 CN CN201610163915.3A patent/CN105724254B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN105724254A (en) | 2016-07-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fki et al. | Multiple bud cultures of ‘Barhee’date palm (Phoenix dactylifera) and physiological status of regenerated plants | |
| CN105284620B (en) | A kind of method that Superearly peach bybrid embryo saves seedling | |
| JP6316545B2 (en) | Cutting seedling production method | |
| CN104221859B (en) | A kind of fast numerous cultural method of Garcinia mangostana | |
| CN101933455A (en) | In vitro propagation method for cinnamomum japonicum | |
| CN103782903A (en) | Efficient regeneration method for anthocephalus chinensis plants | |
| CN103299901A (en) | In-vitro rapid proliferation method of Masui dauphine fig | |
| CN108142283A (en) | A kind of tissue culture and rapid propagation method of acer catalpifolium | |
| Rahman et al. | A biotechnological approach for the production of red gerbera (Gerbera jamesonii Bolus) | |
| CN105724254B (en) | A kind of Fraxinus velutina excised leaf body embryo inducing culture | |
| Bhatt et al. | In vitro morphogenesis studies in gerbera jamesonii bolus ex hooker F. | |
| Jevremović et al. | Clonal fidelity of Chrysanthemum cultivars after long term micropropagation by stem segment culture | |
| AbdAlla et al. | In vitro propagation of blackberry (Rubus fruticosus L.) | |
| CN101904302B (en) | Method for somatic cell embryogeny and plant regeneration of medicinal plant schisandga chinensis baill | |
| Majidian et al. | The effect of four levels of GA3 and BA on the quantitative and qualitative characteristics of Zantedeschia aethiopica cv. Childsiana pot plant | |
| CN108142284A (en) | A kind of tissue culture and rapid propagation method of five leaflets maple | |
| Kane et al. | In vitro propagation of Cryptocoryne wendtii | |
| KR101471923B1 (en) | Embryogenic tissue induction from immature seeds in loblolly pine (Pinus taeda) | |
| CN103947547B (en) | A kind of improvement manufacture method of submerged plant artificial endosperm | |
| CN106613973B (en) | Utilize the method for tissue-cultured seedling leaf regeneration adventitious bud fast breeding Chinese azalea | |
| KR101681062B1 (en) | Optimal medium composition for tissue culture of Chrysanthemum morifolium 'Baekma' and tissue culture method using the same | |
| Lyubomirova et al. | In vitro propagation of Syringa vulgaris L | |
| CN106718846A (en) | A kind of breeding method of orchid | |
| Eganathan et al. | Micropropagation of Sauropus androgynus (L.) Merr.—An important green leafy vegetable | |
| CN108260525B (en) | Tissue culture medium and tissue culture method for golden ball thuja sempervirens |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |