A kind of collection liquid and application thereof for protecting and stablize hemoglobin in feces
Technical field
The invention belongs to biologic applications technical field, be used for protecting and stablizing in feces particularly to one
The collection liquid of hemoglobin and application thereof.
Background technology
" stool blood " refers to digestive tract hemorrhage amount every day and is less than 5ml, has numerous disease such as: knot
Rectal cancer, colitis, digestive tract ulcer, polyp intestinal, the disease such as gastrointestinal diverticulum is the most asymptomatic,
Only have stool blood.Colorectal cancer refers to that focus is limited to mucous layer or tela submucosa and does not invades and flesh in early days
Layer person, is often the asymptomatic stage, will not actively see a doctor, to high-risk group's examination and make regular check on and be
Find the important way of early cancer patient.Clinical research confirmation, the alimentary tumor patient of 87% is just occulted blood
Test is the positive;Trace is hemorrhage, is that Early cancer is the most permissible with Trace Blood Lactoferrin in feces
The abnormal phenomena found, so having great importance to the detection of Trace Blood Lactoferrin in feces.And
Early finding early cancer patient, and treat in early days, its long term survival rate is up to 91%.For wherein
Focus limitation person even can retain the anus with physiological function, considerably reduces the misery of patient,
Improve quality of life.Therefore, recommend in many developed countries such as the U.S.: annual more than 50 years old crowd
Periodically do a stool blood inspection, wherein positive patient is remake further close examination.
Bottleneck for detecting stool blood maximum is the collecting dung liquid holding time not the most on the market
Long, reduce quickly than relatively low specimen, antigen valence especially for hemoglobin.But for detection
For mechanism or personnel, the fresh specimens collected generally requires 1 day one day and the above time of placement
Detect, and this is the highest for the requirement of collecting dung liquid.The fecal occult blood test of present invention exploitation
Reagent collecting dung liquid, has preferably protection and Stabilization, fresh receipts to the hemoglobin in feces
The specimen of collection can be placed 7 days at normal temperatures, for domestic initiation, fills up the blank in this field domestic.
Summary of the invention
It is an object of the invention to as solving the problems referred to above, it is desirable to provide one can be protected and stablize in feces
The solution formula of hemoglobin.
The present invention is for protecting and stablize the collection liquid of hemoglobin in feces, including chelating agen, anticorrosion
Agent, sealer, buffer agent, Polyethylene Glycol, each component concentration in described solution be respectively 25~
45g/L, 0.2~1g/L, 1~10g/L, 6~12g/L, 25~30g/L.
Described is 9.4~9.5 for protecting and stablizing the pH value collecting liquid of hemoglobin in feces.
Described chelating agen is 3-hydroxyl-2,2 ' iminodisuccinic acid four sodium (HIDS), ethylenediamine tetraacetic
Two kinds of mixture in acetic acid (EDTA), sodium tripolyphosphate.
Described preservative is the mixture of Proclin 150 and Proclin 300;
Described sealer is the one in bSA, casein;
Described buffer agent is the one in trishydroxymethylaminomethane Tris, disodium hydrogen phosphate, Borax;
As preferably, the described collection liquid being used for protecting and stablizing hemoglobin in feces is by 3-hydroxyl
Base-2,2 ' iminodisuccinic acid four sodium (HIDS), ethylenediaminetetraacetic acid (EDTA), Proclin 300,
Proclin 150, trishydroxymethylaminomethane Tris, calf blood are pure, Polyethylene Glycol, purified water
Composition, HIDS, EDTA, Proclin 300, Proclin 150, Tris, bSA,
Polyethylene Glycol concentration in described solution be respectively 35g/L, 10g/L, 0.2g/L, 0.2g/L,
6.05g/L、1g/L、25g/L。
It is a further object to provide above-mentioned collection liquid answering in pretreatment before feces detects
With.
The present invention is placed in after being mixed homogeneously in 50mg:2ml ratio with above-mentioned collection liquid by fresh excreta
Room temperature (7 days), 2-8 DEG C (1 month) ,-20 DEG C (6 months) preserve.
The method preparing stool blood collection liquid of the present invention, specifically comprises the following steps that
1) each component is weighed by formula;
2) be initially charged the purified water of 700ml in container, by step 1) in each component according to following
Order is sequentially added into: after buffer agent, sealer, chelating agen, Polyethylene Glycol, preservative mix homogeneously,
It is settled to 1L, adjusts pH to 9.4~9.5, obtain stool blood and collect liquid.
The present invention collects liquid so that the hemoglobin in feces is more stable in this protection liquid, protects
Deposit the time longer.
Detailed description of the invention
Following example are used for explaining the present invention, rather than limit the present invention.
Comparative example 1:
It is initially charged the purified water of 70ml in container, then sequentially adds 0.605g trihydroxy methyl ammonia
Methylmethane Tris, 1g bSA, 3.5g 3-hydroxyl-2,2 ' iminodisuccinic acid four sodium
(HIDS), 2.5g Polyethylene Glycol, 0.02ml Proclin 150,0.02ml Proclin 300,
It is settled to 100mL after mix homogeneously, adjusts pH to 9.4, obtain stool blood and collect liquid.
Comparative example 2:
It is initially charged the purified water of 70ml in container, then sequentially adds 0.605g trihydroxy methyl ammonia
Methylmethane Tris, 1g bSA, 1g ethylenediaminetetraacetic acid (EDTA), the poly-second of 2.5g two
Alcohol, 0.02ml Proclin 150,0.02ml Proclin 300, be settled to 100mL after mix homogeneously,
Adjust pH to 9.4, obtain stool blood and collect liquid.
Embodiment 1:
It is initially charged the purified water of 70ml in container, then sequentially adds 0.605g trihydroxy methyl ammonia
Methylmethane Tris, 1g bSA, 3.5g 3-hydroxyl-2,2 ' iminodisuccinic acid four sodium
(HIDS), 1g ethylenediaminetetraacetic acid (EDTA), 2.5g Polyethylene Glycol, 0.02ml Proclin 150,
0.02ml Proclin 300, is settled to 100mL after mix homogeneously, adjust pH to 9.4, obtains big
Just occult blood collection liquid.
Embodiment 2:
It is initially charged the purified water of 70ml in container, then sequentially adds 0.6g trihydroxy methyl amino
Methane Tris, 0.5g bSA, 1g 3-hydroxyl-2,2 ' iminodisuccinic acid four sodium
(HIDS), 1.5g ethylenediaminetetraacetic acid (EDTA), 2.5g Polyethylene Glycol, 0.01g Proclin 150,
0.01g Proclin 300, is settled to 100mL after mix homogeneously, adjust pH to 9.4, defecated
Occult blood collection liquid.
Embodiment 3:
Be initially charged the purified water of 70ml in container, then sequentially add 1.2g disodium hydrogen phosphate,
0.5g casein, 2g 3-hydroxyl-2,2 ' iminodisuccinic acid four sodium (HIDS), 2g tripolyphosphate
Sodium, 3g Polyethylene Glycol, 0.5g Proclin 150,0.5g Proclin 300, fixed after mix homogeneously
Hold to 100mL, adjust pH to 9.5, obtain stool blood and collect liquid.
Embodiment 4:
It is initially charged the purified water of 70ml in container, then sequentially adds 0.8g Borax, 1g calf
Serum albumin, 1g ethylenediaminetetraacetic acid (EDTA), 2g sodium tripolyphosphate, 2.6g Polyethylene Glycol,
0.25g Proclin 150,0.25g Proclin 300, be settled to 100mL after mix homogeneously, adjusts
Whole pH to 9.5, obtains stool blood and collects liquid.
Embodiment 5:
It is initially charged the purified water of 70ml in container, then sequentially adds 1g trihydroxy methyl amino first
Alkane Tris, 0.2g bSA, 2g 3-hydroxyl-2,2 ' iminodisuccinic acid four sodium
(HIDS), 1.5g ethylenediaminetetraacetic acid (EDTA), 2.8g Polyethylene Glycol, 0.03g Proclin 150,
0.03g Proclin 300, is settled to 100mL after mix homogeneously, adjust pH to 9.4, defecated
Occult blood collection liquid.
Application Example 1:
Taking the most conventional use of several stool blood collecting dung liquid at present, it is that A is public that A collects liquid
The collection liquid that department provides, formula is made up of EDTA and sodium chloride, and wherein concentration is respectively 3~10g/L
With 5~20g/L;B collects the collection liquid that liquid provides for B company, and formula is by HEPES, calf serum
Albumin, sodium chloride, sucrose, preservative form, wherein concentration be respectively 0.5~5g/L, 1~10g/L,
5~20g/L, 5~30g/L, 0.2~1g/L.
Take 50mg fresh stool blood positive stool sample respectively and join tri-kinds of collecting dungs of above-mentioned 2ml
In liquid, and be placed into room temperature, 2-8 DEG C and-20 DEG C of preservations, and in 0 day, 3 days, 7 days, 10 days,
15 days, 30 days, 2 months, 3 months, 4 months, 5 months, 6 months, 9 months, 12 months
Assessment performance, is shown in Table 1.
Table 1 Application Example uses different collection liquid to carry out stool and preserves assessment result
Note: "+" representing positive findings, "-" represents negative findings.
The above results shows, the collection liquid that embodiment 1 prepares can store 7 days at normal temperatures, 2-8 DEG C
Lower storage 30 days, stores 6 months at-20 DEG C;Comparative example A collect liquid, B collect liquid, comparative example 1,
The collection liquid that the stablizing effect of comparative example 2 hemoglobin substantially prepares not as good as embodiment 1.
Above-described embodiment is not the restriction for the present invention, and the present invention is not limited only to above-described embodiment,
As long as meeting application claims, belong to protection scope of the present invention.