CN105716917A - Collection liquid for protecting and stabilizing hemoglobin in manure and application of collection liquid - Google Patents
Collection liquid for protecting and stabilizing hemoglobin in manure and application of collection liquid Download PDFInfo
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- CN105716917A CN105716917A CN201610045537.9A CN201610045537A CN105716917A CN 105716917 A CN105716917 A CN 105716917A CN 201610045537 A CN201610045537 A CN 201610045537A CN 105716917 A CN105716917 A CN 105716917A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention discloses collection liquid for protecting and stabilizing hemoglobin in manure and application of the collection liquid. The collection liquid comprises 25-45 g/L of chelating agent, 0.2-1 g/L of preservative, 1-10 g/L of sealing agent, 6-12 g/L of buffering agent and 25-30 g/L of polyethylene glycol, wherein the chelating agent is a mixture of two of HIDS, EDTA and sodium tripolyphosphate. After fresh manure and the collection liquid are mixed well according to a proportion of 50 mg:2 ml, the collection liquid can be stored for 7 days at normal temperature, one month at 2-8 DEG C and six months at minus 20 DEG C. The collection liquid can further stabilize hemoglobin and prolong storage time of hemoglobin in manure in protection liquid.
Description
Technical field
The invention belongs to biologic applications technical field, be used for protecting and stablizing in feces particularly to one
The collection liquid of hemoglobin and application thereof.
Background technology
" stool blood " refers to digestive tract hemorrhage amount every day and is less than 5ml, has numerous disease such as: knot
Rectal cancer, colitis, digestive tract ulcer, polyp intestinal, the disease such as gastrointestinal diverticulum is the most asymptomatic,
Only have stool blood.Colorectal cancer refers to that focus is limited to mucous layer or tela submucosa and does not invades and flesh in early days
Layer person, is often the asymptomatic stage, will not actively see a doctor, to high-risk group's examination and make regular check on and be
Find the important way of early cancer patient.Clinical research confirmation, the alimentary tumor patient of 87% is just occulted blood
Test is the positive；Trace is hemorrhage, is that Early cancer is the most permissible with Trace Blood Lactoferrin in feces
The abnormal phenomena found, so having great importance to the detection of Trace Blood Lactoferrin in feces.And
Early finding early cancer patient, and treat in early days, its long term survival rate is up to 91%.For wherein
Focus limitation person even can retain the anus with physiological function, considerably reduces the misery of patient,
Improve quality of life.Therefore, recommend in many developed countries such as the U.S.: annual more than 50 years old crowd
Periodically do a stool blood inspection, wherein positive patient is remake further close examination.
Bottleneck for detecting stool blood maximum is the collecting dung liquid holding time not the most on the market
Long, reduce quickly than relatively low specimen, antigen valence especially for hemoglobin.But for detection
For mechanism or personnel, the fresh specimens collected generally requires 1 day one day and the above time of placement
Detect, and this is the highest for the requirement of collecting dung liquid.The fecal occult blood test of present invention exploitation
Reagent collecting dung liquid, has preferably protection and Stabilization, fresh receipts to the hemoglobin in feces
The specimen of collection can be placed 7 days at normal temperatures, for domestic initiation, fills up the blank in this field domestic.
Summary of the invention
It is an object of the invention to as solving the problems referred to above, it is desirable to provide one can be protected and stablize in feces
The solution formula of hemoglobin.
The present invention is for protecting and stablize the collection liquid of hemoglobin in feces, including chelating agen, anticorrosion
Agent, sealer, buffer agent, Polyethylene Glycol, each component concentration in described solution be respectively 25～
45g/L, 0.2～1g/L, 1～10g/L, 6～12g/L, 25～30g/L.
Described is 9.4～9.5 for protecting and stablizing the pH value collecting liquid of hemoglobin in feces.
Described chelating agen is 3-hydroxyl-2,2 ' iminodisuccinic acid four sodium (HIDS), ethylenediamine tetraacetic
Two kinds of mixture in acetic acid (EDTA), sodium tripolyphosphate.
Described preservative is the mixture of Proclin 150 and Proclin 300；
Described sealer is the one in bSA, casein；
Described buffer agent is the one in trishydroxymethylaminomethane Tris, disodium hydrogen phosphate, Borax；
As preferably, the described collection liquid being used for protecting and stablizing hemoglobin in feces is by 3-hydroxyl
Base-2,2 ' iminodisuccinic acid four sodium (HIDS), ethylenediaminetetraacetic acid (EDTA), Proclin 300,
Proclin 150, trishydroxymethylaminomethane Tris, calf blood are pure, Polyethylene Glycol, purified water
Composition, HIDS, EDTA, Proclin 300, Proclin 150, Tris, bSA,
Polyethylene Glycol concentration in described solution be respectively 35g/L, 10g/L, 0.2g/L, 0.2g/L,
6.05g/L、1g/L、25g/L。
It is a further object to provide above-mentioned collection liquid answering in pretreatment before feces detects
With.
The present invention is placed in after being mixed homogeneously in 50mg:2ml ratio with above-mentioned collection liquid by fresh excreta
Room temperature (7 days), 2-8 DEG C (1 month) ,-20 DEG C (6 months) preserve.
The method preparing stool blood collection liquid of the present invention, specifically comprises the following steps that
1) each component is weighed by formula；
2) be initially charged the purified water of 700ml in container, by step 1) in each component according to following
Order is sequentially added into: after buffer agent, sealer, chelating agen, Polyethylene Glycol, preservative mix homogeneously,
It is settled to 1L, adjusts pH to 9.4～9.5, obtain stool blood and collect liquid.
The present invention collects liquid so that the hemoglobin in feces is more stable in this protection liquid, protects
Deposit the time longer.
Detailed description of the invention
Following example are used for explaining the present invention, rather than limit the present invention.
Comparative example 1:
It is initially charged the purified water of 70ml in container, then sequentially adds 0.605g trihydroxy methyl ammonia
Methylmethane Tris, 1g bSA, 3.5g 3-hydroxyl-2,2 ' iminodisuccinic acid four sodium
(HIDS), 2.5g Polyethylene Glycol, 0.02ml Proclin 150,0.02ml Proclin 300,
It is settled to 100mL after mix homogeneously, adjusts pH to 9.4, obtain stool blood and collect liquid.
Comparative example 2:
It is initially charged the purified water of 70ml in container, then sequentially adds 0.605g trihydroxy methyl ammonia
Methylmethane Tris, 1g bSA, 1g ethylenediaminetetraacetic acid (EDTA), the poly-second of 2.5g two
Alcohol, 0.02ml Proclin 150,0.02ml Proclin 300, be settled to 100mL after mix homogeneously,
Adjust pH to 9.4, obtain stool blood and collect liquid.
Embodiment 1:
It is initially charged the purified water of 70ml in container, then sequentially adds 0.605g trihydroxy methyl ammonia
Methylmethane Tris, 1g bSA, 3.5g 3-hydroxyl-2,2 ' iminodisuccinic acid four sodium
(HIDS), 1g ethylenediaminetetraacetic acid (EDTA), 2.5g Polyethylene Glycol, 0.02ml Proclin 150,
0.02ml Proclin 300, is settled to 100mL after mix homogeneously, adjust pH to 9.4, obtains big
Just occult blood collection liquid.
Embodiment 2:
It is initially charged the purified water of 70ml in container, then sequentially adds 0.6g trihydroxy methyl amino
Methane Tris, 0.5g bSA, 1g 3-hydroxyl-2,2 ' iminodisuccinic acid four sodium
(HIDS), 1.5g ethylenediaminetetraacetic acid (EDTA), 2.5g Polyethylene Glycol, 0.01g Proclin 150,
0.01g Proclin 300, is settled to 100mL after mix homogeneously, adjust pH to 9.4, defecated
Occult blood collection liquid.
Embodiment 3:
Be initially charged the purified water of 70ml in container, then sequentially add 1.2g disodium hydrogen phosphate,
0.5g casein, 2g 3-hydroxyl-2,2 ' iminodisuccinic acid four sodium (HIDS), 2g tripolyphosphate
Sodium, 3g Polyethylene Glycol, 0.5g Proclin 150,0.5g Proclin 300, fixed after mix homogeneously
Hold to 100mL, adjust pH to 9.5, obtain stool blood and collect liquid.
Embodiment 4:
It is initially charged the purified water of 70ml in container, then sequentially adds 0.8g Borax, 1g calf
Serum albumin, 1g ethylenediaminetetraacetic acid (EDTA), 2g sodium tripolyphosphate, 2.6g Polyethylene Glycol,
0.25g Proclin 150,0.25g Proclin 300, be settled to 100mL after mix homogeneously, adjusts
Whole pH to 9.5, obtains stool blood and collects liquid.
Embodiment 5:
It is initially charged the purified water of 70ml in container, then sequentially adds 1g trihydroxy methyl amino first
Alkane Tris, 0.2g bSA, 2g 3-hydroxyl-2,2 ' iminodisuccinic acid four sodium
(HIDS), 1.5g ethylenediaminetetraacetic acid (EDTA), 2.8g Polyethylene Glycol, 0.03g Proclin 150,
0.03g Proclin 300, is settled to 100mL after mix homogeneously, adjust pH to 9.4, defecated
Occult blood collection liquid.
Application Example 1:
Taking the most conventional use of several stool blood collecting dung liquid at present, it is that A is public that A collects liquid
The collection liquid that department provides, formula is made up of EDTA and sodium chloride, and wherein concentration is respectively 3～10g/L
With 5～20g/L；B collects the collection liquid that liquid provides for B company, and formula is by HEPES, calf serum
Albumin, sodium chloride, sucrose, preservative form, wherein concentration be respectively 0.5～5g/L, 1～10g/L,
5～20g/L, 5～30g/L, 0.2～1g/L.
Take 50mg fresh stool blood positive stool sample respectively and join tri-kinds of collecting dungs of above-mentioned 2ml
In liquid, and be placed into room temperature, 2-8 DEG C and-20 DEG C of preservations, and in 0 day, 3 days, 7 days, 10 days,
15 days, 30 days, 2 months, 3 months, 4 months, 5 months, 6 months, 9 months, 12 months
Assessment performance, is shown in Table 1.
Table 1 Application Example uses different collection liquid to carry out stool and preserves assessment result
Note: "+" representing positive findings, "-" represents negative findings.
The above results shows, the collection liquid that embodiment 1 prepares can store 7 days at normal temperatures, 2-8 DEG C
Lower storage 30 days, stores 6 months at-20 DEG C；Comparative example A collect liquid, B collect liquid, comparative example 1,
The collection liquid that the stablizing effect of comparative example 2 hemoglobin substantially prepares not as good as embodiment 1.
Above-described embodiment is not the restriction for the present invention, and the present invention is not limited only to above-described embodiment,
As long as meeting application claims, belong to protection scope of the present invention.
Claims (10)
1. the collection liquid being used for protecting and stablize hemoglobin in feces, it is characterised in that bag
Including chelating agen, preservative, sealer, buffer agent, Polyethylene Glycol, each component is in described solution
Concentration be respectively 25～45g/L, 0.2～1g/L, 1～10g/L, 6～12g/L, 25～30g/L.
A kind of for protecting and stablizing the receipts of hemoglobin in feces
Liquid collecting, it is characterised in that described for protect and stablize the stabilizer of hemoglobin in feces be by
3-hydroxyl-2,2/Iminodisuccinic acid four sodium (HIDS), ethylenediaminetetraacetic acid (EDTA), Proclin
300, Proclin 150, trishydroxymethylaminomethane Tris, calf blood be pure, Polyethylene Glycol,
Purified water forms, HIDS, EDTA, Proclin 300, Proclin 150, Tris, calf
Serum albumin, the Polyethylene Glycol concentration in described solution be respectively 35g/L, 10g/L,
0.2g/L、0.2g/L、6.05g/L、1g/L、25g/L。
A kind of for protecting and stablizing the receipts of hemoglobin in feces
Liquid collecting, it is characterised in that described chelating agen is 3-hydroxyl-2,2/Iminodisuccinic acid four sodium
(HIDS), two kinds of mixture in ethylenediaminetetraacetic acid (EDTA), sodium tripolyphosphate.
A kind of for protecting and stablizing the receipts of hemoglobin in feces
Liquid collecting, it is characterised in that described preservative is the mixing of Proclin 150 and Proclin 300
Thing.
A kind of for protecting and stablizing the receipts of hemoglobin in feces
Liquid collecting, it is characterised in that described sealer is the one in bSA, casein.
A kind of for protecting and stablizing the receipts of hemoglobin in feces
Liquid collecting, it is characterised in that described buffer agent is trishydroxymethylaminomethane Tris, disodium hydrogen phosphate,
One in Borax.
7. the one as described in as arbitrary in claim 1～6 is used for protecting and stablize blood red egg in feces
White collection liquid, it is characterised in that the pH value of this collection liquid is 9.4～9.5.
A kind of for protecting and stablizing the receipts of hemoglobin in feces
Liquid collecting is the application in pretreatment before feces detects.
Apply the most as claimed in claim 8, it is characterised in that fresh excreta is wanted with such as right
Ask collection liquid described in 1 in be placed in after 50mg:2ml ratio mix homogeneously room temperature, 2～8 DEG C or
Preserve at-20 DEG C.
Apply the most as claimed in claim 9, it is characterised in that the time preserved at normal temperatures is long
Reach 7 days；At 2～8 DEG C preserve for up to 1 month；The time preserved at-20 DEG C is long
Reach 6 months.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610045537.9A CN105716917B (en) | 2016-01-22 | 2016-01-22 | collecting liquid for protecting and stabilizing hemoglobin in feces and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610045537.9A CN105716917B (en) | 2016-01-22 | 2016-01-22 | collecting liquid for protecting and stabilizing hemoglobin in feces and application thereof |
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| Publication Number | Publication Date |
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| CN105716917A true CN105716917A (en) | 2016-06-29 |
| CN105716917B CN105716917B (en) | 2020-01-31 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106769289A (en) * | 2016-12-02 | 2017-05-31 | 刘鹏飞 | A kind of collocation method of fecal specimens storage liquid and application |
| CN108840926A (en) * | 2018-07-12 | 2018-11-20 | 中科凤祥生物工程股份有限公司 | The high efficiency preparation method of liquid Poultry blood hemoglobin |
| CN111004831A (en) * | 2019-12-31 | 2020-04-14 | 阿吉安(福州)基因医学检验实验室有限公司 | Excrement storage liquid and preparation method and application thereof |
| CN111879924A (en) * | 2020-01-15 | 2020-11-03 | 杭州奥泰生物技术股份有限公司 | Colloidal gold immunochromatography test paper for rapidly diagnosing hemoglobin and combining globin-hemoglobin compound and preparation method thereof |
| TWI842742B (en) * | 2018-09-19 | 2024-05-21 | 日商榮研化學股份有限公司 | Method for managing the accuracy of fecal occult blood tests using simulated feces |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106769289A (en) * | 2016-12-02 | 2017-05-31 | 刘鹏飞 | A kind of collocation method of fecal specimens storage liquid and application |
| CN108840926A (en) * | 2018-07-12 | 2018-11-20 | 中科凤祥生物工程股份有限公司 | The high efficiency preparation method of liquid Poultry blood hemoglobin |
| TWI842742B (en) * | 2018-09-19 | 2024-05-21 | 日商榮研化學股份有限公司 | Method for managing the accuracy of fecal occult blood tests using simulated feces |
| CN111004831A (en) * | 2019-12-31 | 2020-04-14 | 阿吉安(福州)基因医学检验实验室有限公司 | Excrement storage liquid and preparation method and application thereof |
| CN111004831B (en) * | 2019-12-31 | 2022-10-21 | 阿吉安(福州)基因医学检验实验室有限公司 | Excrement storage liquid and preparation method and application thereof |
| CN111879924A (en) * | 2020-01-15 | 2020-11-03 | 杭州奥泰生物技术股份有限公司 | Colloidal gold immunochromatography test paper for rapidly diagnosing hemoglobin and combining globin-hemoglobin compound and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN105716917B (en) | 2020-01-31 |
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