CN105713842B - A method of cigarette perfume of jasmines is prepared using trichoderma aureoviride fermentation - Google Patents
A method of cigarette perfume of jasmines is prepared using trichoderma aureoviride fermentation Download PDFInfo
- Publication number
- CN105713842B CN105713842B CN201610239576.2A CN201610239576A CN105713842B CN 105713842 B CN105713842 B CN 105713842B CN 201610239576 A CN201610239576 A CN 201610239576A CN 105713842 B CN105713842 B CN 105713842B
- Authority
- CN
- China
- Prior art keywords
- fermentation
- trichoderma aureoviride
- jasmine
- hlmm
- trichoderma
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/885—Trichoderma
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
Abstract
The invention discloses a kind of methods for preparing cigarette perfume of jasmines using trichoderma aureoviride fermentation.The bacterial strain is trichoderma aureoviride (Trichoderma aureoviride) HLMM-04 CGMCC No.10839, has cellulase-producing, and then promote cellulose decomposition at the ability of oligosaccharides or monosaccharide.Bacterial strain is passed through into activation, is fermented, filtering, obtained cellulase crude enzyme liquid of dialysing, tobacco aromaticss are made for Jasmine of fermenting, then extracted, filtering, reduced pressure.The Liquid fermentation condition of HLMM-04 bacterial strain is that the initial pH of culture medium is 6.5, and cultivation temperature is 28 DEG C, incubation time 5d.Flavoring the result shows that, prepared fragrance have the function of abundant fragrance, increase the cigarette fragrance of a flower.
Description
Technical field
The invention belongs to microbial fermentation and its application fields, and in particular to one plant of trichoderma aureoviride new strains.Meanwhile this hair
The bright method for further relating to prepare cigarette perfume of jasmines using the bacterial strain.
Background technique
Flavoring and casing is the key link in cigarette process, is effective promotion cigarette smoking quality, forms the peculiar wind of cigarette
One of effective means of lattice.The tobacco aromaticss added in China tobacco at present are deployed again using conventional method extraction mostly
Gained there is also fragrance dullness, improves the disadvantages of cigarette smoking effect is unobvious although production operation is simple.Utilize micro- life
Object, which ferments to perfume material, can increase the content that tradition extracts a variety of aroma substances in fragrance, and promotion fragrance is rich, into
And produce that fragrance is abundant, cigarette of high-quality, meet requirement of the consumer to cigarette quality.
Jasmine, Latin literary fame: Jasminum sambac (L.) Ait, Oleaceae shrub.The month at florescence 5-8, the fruiting period 7-9 month.
The Hua Jixiang of jasmine is famous jasmine tea raw material and important flavor of raw material；Flower, leaf is medicinal controls red eye, swell pain, and has cough-relieving
The benefits of phlegm.
Trichoderma (Trichoderma spp.) is distributed widely in nature, rotten wood, seed, plant residue, organic fertilizer,
It is outstanding more in soil and air.Trichoderma growing nutrient requires simply, and the speed of growth is fast, highly beneficial for industrialized production.It is yellow
Green trichoderma (Trichoderma aureoviride) is one kind of trichoderma, as a kind of important bio-control factors, to phytopathy
Former antagonism has broad spectrum activity, property mostly processed, moreover it is possible to generate the plant and animal residues in a variety of enzymes decomposition soil, increase soil
Fertility.Have to the report of trichoderma aureoviride focus mostly on soil improvement, biological control and in terms of.In the prior art
There is not yet trichoderma aureoviride is used for the report that Jasmine fermentation prepares tobacco aromaticss.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide one plant of trichoderma aureoviride new strains, which has
Cellulase-producing, and then promote cellulose decomposition at the ability of oligosaccharides or monosaccharide.
The object of the invention is also to provide a kind of methods using strain fermentation preparation Jasmine tobacco aromaticss.
The purpose of the present invention is achieved by the following technical programs.
Unless otherwise indicated, percentage of the present invention is mass percent.
A kind of trichoderma aureoviride (Trichoderma aureoviride) HLMM-04 bacterial strain, CGMCC No.10839, tool
There is cellulase-producing, and then promotes cellulose decomposition at the ability of oligosaccharides or monosaccharide.
The method for preparing tobacco aromaticss using the bacterial strain, comprising the following steps:
(1) it by after the activation twice of trichoderma aureoviride HLMM-04 CGMCC No.10839 bacterial strain, is inoculated in equipped with 200mL PDA
In the triangular flask of fluid nutrient medium, at 20~35 DEG C, 1~7d of fermented and cultured under 180rpm；The PDA liquid medium: horse
Bell potato 200g, glucose 20g mend distilled water to 1L, pH5.5~8.5,115 DEG C of high pressure sterilization 15min；
(2) filtering fermentation liquor removes thallus, takes supernatant to be slowly added to sulfate of ammoniac to 80% saturation degree, stood at 4 DEG C
Night is collected by centrifugation sediment and is dissolved in 2mL buffer, then dialysed with buffer, obtains crude enzyme liquid；The buffer
Are as follows: 0.02mol/L Na2HPO4-NaH2PO4, pH 7.3；
(3) dried jasmine flower piece is carried out after being crushed, sprays trichoderma aureoviride HLMM-04 crude enzyme liquid, fermentation, crude enzyme liquid additive amount
It is the 20% of jasmine flower quality, fermentation temperature is 30 DEG C, fermentation time 5h；Add the water of double weight, refluxing extraction 3h,
Supernatant is concentrated under reduced pressure to get required tobacco aromaticss filtering and removing slag.
Further, to prevent fragrance rotten, propylene glycol is added by the 5% of tobacco aromaticss weight.
The fragrant condition of optimum production is initial pH 6.5 in step (1), 28 DEG C of cultivation temperature, incubation time 5d.
Compared with the existing technology, the invention has the following advantages that
(1) trichoderma aureoviride is applied to tobacco aromaticss preparation field for the first time by the present invention.Using trichoderma aureoviride HLMM-04 bacterial strain
Fermentation liquor treatment Jasmine, compared with untreated control, while original Jasmine characteristic perfume is kept, the rich increasing of fragrance
Add, the fragrance of a flower is more prominent.
(2) present invention is low in cost, easy to operate, is easy to implement large-scale industrial production, application prospect is good.
The explanation of preservation biomaterial
It is common to be deposited in China Committee for Culture Collection of Microorganisms to bacterial strain of the invention on May 22nd, 2015
Microorganism center (CGMCG)；The centre address: city, BeiJing, China, North Star West Road 1, Chaoyang District institute 3, the micro- life of the Chinese Academy of Sciences
Object research institute.The strain classification is named as trichoderma aureoviride (Trichoderma aureoviride) HLMM-04, and deposit number is
CGMCC No.10839。
Specific embodiment
Below by embodiment, the present invention is described in further detail, but embodiment is not to the technology of the present invention side
The restriction of case.
Embodiment 1
1, the suitable condition of enzyme production of trichoderma aureoviride HLMM-04 bacterial strain
Influence of the initial pH of 1.1 culture mediums to HLMM-04 strain enzyme-producing
The pH of fluid nutrient medium is transferred to 5.5,6.0,6.5,7.0,7.5,8.0,8.5 respectively, is shaken at 28 DEG C, 120rpm
After swinging culture 3 days, cellulase activity is measured, shows that optimum initial pH is 6.5.
Influence of 1.2 cultivation temperatures to HLMM-04 strain enzyme-producing
Liquid fermentation medium after inoculation is respectively placed at 20 DEG C, 25 DEG C, 28 DEG C, 32 DEG C, 35 DEG C, 120r/min vibration
After swinging culture 3d, cellulase activity is measured, shows that optimum cultivation temperature is 28 DEG C.
Influence of 1.3 incubation times to HLMM-04 strain enzyme-producing
Inoculated fluid nutrient medium (pH 6.5) is placed in climatic chamber, 28 DEG C, 120r/min shaken cultivation, often
Primary fiber element enzyme activity is measured for 24 hours, and cellulase activity reaches maximum value when culture was by the 5th day.
2, trichoderma aureoviride HLMM-04 crude enzyme liquid preparation method
It is inoculated in after the activation twice of trichoderma aureoviride HLMM-04 bacterial strain equipped with 200mL PDA according to the above optimum results
In the triangular flask of fluid nutrient medium (pH 6.5), at 28 DEG C, fermented and cultured 5d under 180rpm.Filtering removal thallus.Take supernatant
Sulfate of ammoniac is slowly added to 80% saturation degree, is stood overnight at 4 DEG C, sediment is collected by centrifugation and is dissolved in 2mL buffer
(0.02mol/L Na2HPO4-NaH2PO4Buffer, pH 7.3) in, dialysed with same buffer, obtain thick enzyme
Liquid.
3, the method that trichoderma aureoviride HLMM-04 strain fermentation prepares tobacco aromaticss
Dried jasmine flower piece is carried out after being suitably crushed, trichoderma aureoviride HLMM-04 crude enzyme liquid, fermentation, crude enzyme liquid addition are sprayed
Amount is the 20% of Jasmine quality, and fermentation temperature is 30 DEG C, fermentation time 5h；Add the water of double weight, refluxing extraction 3h,
Supernatant is concentrated under reduced pressure to get Jasmine fermentation tobacco aromaticss filtering and removing slag.
To prevent from going bad, by 5% addition propylene glycol of fermentation fragrance weight.
4, product feature and physicochemical property
Gained ferments tobacco aromaticss for rufous stream shape lotion, does not clarify.Product physicochemical property is shown in Table 1.
The physical and chemical index of 1 trichoderma aureoviride HLMM-04 of table fermentation perfume of jasmines
5, perfuming cigarette is tested
Jasmine fermentation tobacco aromaticss 0.1g is taken uniformly to be sprayed at the non-flavoring leaf group of 50g after taking sterile water to dilute 50 times
In, it is packed into clean hermetic bag, after placing 2h at room temperature, pipe tobacco is dried in 120 DEG C of baking ovens, reaches moisture content
11%-13%.Treated pipe tobacco and control are put into climatic chamber, balanced under the conditions of 22 DEG C of temperature, humidity 60%
For 24 hours, cigarette is rolled by hand, and sensory evaluating smoking is carried out by the group of smokeing panel test that the expert that smokes panel test forms.It is extracted with Jasmine of not fermenting fragrant
Material and fermentation of bacillus subtilis perfume of jasmines are control.Flavoring the result shows that, Jasmine trichoderma aureoviride HLMM-04 fermentation is fragrant
Material is obvious with control area, has the function of abundant fragrance, and it is obvious to increase cigarette fragrance of a flower effect.Evaluation result is shown in Table 2.
2 Jasmine of table fermentation fragrance smoking result
Claims (2)
1. a kind of method for preparing Jasmine tobacco aromaticss, comprising the following steps:
(1) trichoderma aureoviride (Trichoderma aureoviride) HLMM-04 bacterium for being CGMCC No.10839 by deposit number
After strain activation twice, it is inoculated in the triangular flask equipped with 200mL PDA liquid medium, in initial pH 6.5, cultivation temperature 28
DEG C, fermented and cultured 5d under 180rpm；The PDA liquid medium: potato 200g, glucose 20g, benefit distilled water to 1L,
PH5.5~8.5,115 DEG C of high pressure sterilization 15min；The trichoderma aureoviride HLMM-04 CGMCC No.10839 bacterial strain has and produces
Cellulase, and then promote cellulose decomposition at the ability of oligosaccharides or monosaccharide；
(2) filtering fermentation liquor removes thallus, takes supernatant to be slowly added to ammonium sulfate to 80% saturation degree, stands overnight at 4 DEG C,
Sediment is collected by centrifugation and is dissolved in 2mL buffer, then is dialysed with buffer, obtains crude enzyme liquid；The buffer are as follows:
0.02mol/L Na2HPO4-NaH2PO4, pH7.3；
(3) dried jasmine flower piece is carried out after being crushed, sprays trichoderma aureoviride HLMM-04 crude enzyme liquid, fermentation, crude enzyme liquid additive amount is jasmine
The 20% of jasmine flower quality, fermentation temperature are 30 DEG C, fermentation time 5h；Add the water of double weight, refluxing extraction 3h, filtering
Supernatant is concentrated under reduced pressure to get required tobacco aromaticss for slagging-off.
2. the method for preparation Jasmine tobacco aromaticss according to claim 1, it is characterised in that: by tobacco aromaticss weight
5% addition propylene glycol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610239576.2A CN105713842B (en) | 2016-04-18 | 2016-04-18 | A method of cigarette perfume of jasmines is prepared using trichoderma aureoviride fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610239576.2A CN105713842B (en) | 2016-04-18 | 2016-04-18 | A method of cigarette perfume of jasmines is prepared using trichoderma aureoviride fermentation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105713842A CN105713842A (en) | 2016-06-29 |
| CN105713842B true CN105713842B (en) | 2019-04-05 |
Family
ID=56160368
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610239576.2A Active CN105713842B (en) | 2016-04-18 | 2016-04-18 | A method of cigarette perfume of jasmines is prepared using trichoderma aureoviride fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105713842B (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1915120A (en) * | 2006-09-07 | 2007-02-21 | 王冰 | Snuff of non-tobacco |
| CN101317692A (en) * | 2008-07-03 | 2008-12-10 | 广西中烟工业公司 | Additive agent for jasmine fragrance type cigarette, preparation and application thereof |
| CN101416778A (en) * | 2008-11-30 | 2009-04-29 | 华南理工大学 | Ferment for tobacco fermentation and use thereof |
| CN102154370A (en) * | 2010-12-13 | 2011-08-17 | 云南瑞升烟草技术（集团）有限公司 | Method for preparing flavor for elsholtzia kachinensis prain tobacco through multi-strain composite fermentation |
| CN102392050A (en) * | 2011-11-03 | 2012-03-28 | 湖南中烟工业有限责任公司 | Endophyte bio-transformed raisin extract and preparation and application in flavoring thereof |
| CN102719313A (en) * | 2011-03-29 | 2012-10-10 | 湖北中烟工业有限责任公司 | A preparation method for a ferment type fruit tobacco flavor |
| CN102894459A (en) * | 2012-10-11 | 2013-01-30 | 湖北中烟工业有限责任公司 | Tobacco leaf conditioning and perfuming method |
| CN103992975A (en) * | 2014-05-28 | 2014-08-20 | 广州市澳键丰泽生物科技有限公司 | Composite aroma-producing microorganism as well as preparation method and application thereof |
| CN104232689A (en) * | 2014-09-30 | 2014-12-24 | 云南中烟工业有限责任公司 | Tobacco flavor prepared by blending and fermenting lily and tobacco |
| CN104479869A (en) * | 2014-12-03 | 2015-04-01 | 湖北中烟工业有限责任公司 | Casing essence for quietly elegant fragrance style cigarette |
| CN104894168A (en) * | 2015-06-18 | 2015-09-09 | 湖北中烟工业有限责任公司 | Extracting method and application of cigarette-used extract from termitornyces albuminosus fermentation substances |
-
2016
- 2016-04-18 CN CN201610239576.2A patent/CN105713842B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1915120A (en) * | 2006-09-07 | 2007-02-21 | 王冰 | Snuff of non-tobacco |
| CN101317692A (en) * | 2008-07-03 | 2008-12-10 | 广西中烟工业公司 | Additive agent for jasmine fragrance type cigarette, preparation and application thereof |
| CN101416778A (en) * | 2008-11-30 | 2009-04-29 | 华南理工大学 | Ferment for tobacco fermentation and use thereof |
| CN102154370A (en) * | 2010-12-13 | 2011-08-17 | 云南瑞升烟草技术（集团）有限公司 | Method for preparing flavor for elsholtzia kachinensis prain tobacco through multi-strain composite fermentation |
| CN102719313A (en) * | 2011-03-29 | 2012-10-10 | 湖北中烟工业有限责任公司 | A preparation method for a ferment type fruit tobacco flavor |
| CN102392050A (en) * | 2011-11-03 | 2012-03-28 | 湖南中烟工业有限责任公司 | Endophyte bio-transformed raisin extract and preparation and application in flavoring thereof |
| CN102894459A (en) * | 2012-10-11 | 2013-01-30 | 湖北中烟工业有限责任公司 | Tobacco leaf conditioning and perfuming method |
| CN103992975A (en) * | 2014-05-28 | 2014-08-20 | 广州市澳键丰泽生物科技有限公司 | Composite aroma-producing microorganism as well as preparation method and application thereof |
| CN104232689A (en) * | 2014-09-30 | 2014-12-24 | 云南中烟工业有限责任公司 | Tobacco flavor prepared by blending and fermenting lily and tobacco |
| CN104479869A (en) * | 2014-12-03 | 2015-04-01 | 湖北中烟工业有限责任公司 | Casing essence for quietly elegant fragrance style cigarette |
| CN104894168A (en) * | 2015-06-18 | 2015-09-09 | 湖北中烟工业有限责任公司 | Extracting method and application of cigarette-used extract from termitornyces albuminosus fermentation substances |
Non-Patent Citations (3)
| Title |
|---|
| "黄绿木霉纤维素酶提纯及其性质研究";宋金柱 等;《哈尔滨工业大学学报》;20061130;第38卷(第11期);第1924页左栏第1.1节，第1.3节，第3段 * |
| Enzymatic properties and anticancer activity of L-lysine α-oxidase from Trichoderma cf. aureoviride Rifai BKMF-4268D.;Pokrovsky VS 等;《Anticancer Drugs》;20130901;第24卷(第8期);第846-851页 * |
| 宋金柱 等."黄绿木霉纤维素酶提纯及其性质研究".《哈尔滨工业大学学报》.2006,第38卷(第11期),第1924页左栏第1.1节，第1.3节，第3段. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105713842A (en) | 2016-06-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102406231B (en) | Fermented rose extract for cigarettes and preparation method thereof | |
| CN107034239A (en) | Tobacco aromaticss and its application prepared by a kind of utilization microbial fermentation worm dung tea | |
| CN105713847B (en) | A kind of trichoderma aureoviride bacterial strain and the method for preparing sanchi flower tobacco aromaticss with it | |
| CN103451108B (en) | Aureobasidium pullulans OF-01 strain and method for preparing tobacco endogenous derived spice by using strain | |
| CN106434359A (en) | Trichoderma aureoviride strain and method for preparing fig cigarette flavor with same | |
| CN109090697B (en) | Method for fermenting tobacco by mixed bacteria | |
| CN102286403A (en) | Bacillus pumilus.Van35 preparation for treating cut tobacco leaves | |
| CN105779307B (en) | A kind of trichoderma aureoviride bacterial strain and the method for preparing Pu'er tea tobacco aromaticss with it | |
| CN107354174A (en) | It is a kind of to utilize tobacco aromaticss of the microbial fermentation walnut every preparation and its application | |
| CN105733962B (en) | A kind of trichoderma aureoviride HLMM-04 bacterial strain and the method for preparing tobacco aromaticss | |
| CN103290065A (en) | Method for preparing borneol by using microorganism separated from natural world | |
| CN108220344A (en) | A kind of tobacco aromaticss prepared using microbial fermentation Stauntonia latifolia and its application | |
| CN107354109A (en) | A kind of tax perfume (or spice) complex microorganism preparations and its application for tobacco leaf Rapid Fermentation | |
| CN105713843B (en) | A kind of trichoderma aureoviride bacterial strain and the method with its preparation Radix Notoginseng tobacco aromatics | |
| CN105713845B (en) | A method of cigarette Yunnan olive fragrance is prepared using trichoderma aureoviride fermentation | |
| CN105713842B (en) | A method of cigarette perfume of jasmines is prepared using trichoderma aureoviride fermentation | |
| CN102618475A (en) | Bacillus methylotrophicus and preparation method as well as application of tobacco cimicifugae extract | |
| CN105713846B (en) | A kind of trichoderma aureoviride bacterial strain and the method with its preparation rose tobacco aromatics | |
| CN105733960B (en) | A kind of trichoderma aureoviride bacterial strain and the method for preparing Dian clove tobacco aromaticss with it | |
| CN105713844B (en) | A kind of trichoderma aureoviride bacterial strain and the method for preparing acid Chinese quince tobacco aromaticss with it | |
| CN108741208A (en) | One plant of flavouring bacterium detached from reproduced tobacco concentrated liquid and its application | |
| CN105754879B (en) | A kind of trichoderma aureoviride bacterial strain and the method for preparing frangipanis tobacco aromaticss with it | |
| CN105861319B (en) | A method of cigarette tamarind fragrance is prepared using trichoderma aureoviride fermentation | |
| CN105733994B (en) | A kind of trichoderma aureoviride bacterial strain and the method for preparing dendrobium candidum tobacco aromaticss with it | |
| CN105733961B (en) | A kind of trichoderma aureoviride bacterial strain and the method for preparing radix scrophulariae tobacco aromaticss with it |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |